##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364624_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2796201 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.591477508233492 31.0 30.0 33.0 26.0 34.0 2 29.705620590222235 31.0 30.0 34.0 26.0 34.0 3 29.817385803094986 31.0 30.0 34.0 26.0 34.0 4 33.532709916061116 35.0 33.0 37.0 28.0 37.0 5 32.90384060373342 35.0 33.0 37.0 26.0 37.0 6 32.94046422270788 35.0 33.0 37.0 26.0 37.0 7 32.74722310735172 35.0 33.0 37.0 25.0 37.0 8 32.689167195062154 35.0 33.0 37.0 25.0 37.0 9 34.00381410349256 38.0 34.0 39.0 25.0 39.0 10-11 33.79470055979523 38.0 33.0 39.0 24.0 39.0 12-13 33.655387076966214 37.0 33.0 39.0 22.5 39.0 14-15 34.65508023207202 39.0 33.0 41.0 23.0 41.0 16-17 34.56370750886649 38.5 33.0 41.0 22.0 41.0 18-19 34.59884321620656 38.5 33.5 41.0 22.0 41.0 20-21 34.616535435042046 39.0 34.0 41.0 22.0 41.0 22-23 34.6057275567815 39.0 34.0 41.0 22.0 41.0 24-25 34.59127634243747 39.0 34.0 41.0 22.5 41.0 26-27 34.48961054659519 39.0 33.5 41.0 22.0 41.0 28-29 34.25820264709154 39.0 33.0 41.0 19.0 41.0 30-31 34.18807821755303 38.0 33.0 41.0 19.5 41.0 32-33 34.008640294456654 38.0 33.0 41.0 18.5 41.0 34-35 33.875936672649786 38.0 33.0 40.5 18.0 41.0 36-37 33.703158499692975 38.0 33.0 40.0 16.0 41.0 38-39 33.568045179870836 38.0 33.0 40.0 15.5 41.0 40-41 33.38189779633152 38.0 32.0 40.0 14.5 41.0 42-43 33.205676380202995 38.0 32.0 40.0 9.5 41.0 44-45 32.99674093529042 38.0 32.0 40.0 8.5 41.0 46-47 32.73923798754095 38.0 31.5 40.0 6.5 41.0 48-49 32.47990130180198 38.0 31.0 40.0 2.0 41.0 50-51 32.272587700240436 38.0 31.0 40.0 2.0 41.0 52-53 32.09809380656111 37.5 31.0 40.0 2.0 41.0 54-55 31.883830776113733 37.0 30.0 40.0 2.0 41.0 56-57 31.65360698318898 37.0 30.0 40.0 2.0 41.0 58-59 31.468973975762115 37.0 30.0 40.0 2.0 41.0 60-61 31.25338790022606 36.0 30.0 40.0 2.0 41.0 62-63 30.987401835561894 36.0 29.0 39.5 2.0 41.0 64-65 30.719230663317838 35.5 29.0 39.0 2.0 41.0 66-67 30.444843378569708 35.0 29.0 39.0 2.0 41.0 68-69 30.123752190919035 35.0 29.0 39.0 2.0 40.0 70-71 29.862642742778505 35.0 29.0 38.0 2.0 40.0 72-73 29.71097446142105 35.0 29.0 37.0 2.0 40.0 74-75 29.429638820671332 35.0 29.0 37.0 2.0 39.0 76-77 29.133777221308485 34.0 28.5 36.5 2.0 39.0 78-79 28.829272645278362 34.0 28.0 36.0 2.0 39.0 80-81 28.556394193407414 34.0 28.0 36.0 2.0 37.5 82-83 28.299767076830314 34.0 28.0 35.0 2.0 37.0 84-85 28.281225491300518 34.0 28.0 35.0 2.0 37.0 86-87 28.14481934596261 34.0 28.0 35.0 2.0 36.0 88-89 27.994701203525786 34.0 28.0 35.0 2.0 36.0 90-91 27.835693321045234 34.0 27.5 35.0 2.0 36.0 92-93 27.707593266721528 34.0 27.0 35.0 2.0 35.5 94-95 27.623513295360382 34.0 27.0 35.0 2.0 35.0 96-97 27.536248824744717 34.0 27.0 35.0 2.0 35.0 98-99 27.443607058290873 34.0 27.0 35.0 2.0 35.0 100-101 27.34883150388688 34.0 27.0 35.0 2.0 35.0 102-103 26.161170101863206 32.0 24.5 34.0 2.0 35.0 104-105 27.09264355459425 33.5 25.5 35.0 2.0 35.0 106-107 27.268173318012543 34.0 27.0 35.0 2.0 35.0 108-109 27.279477762864687 34.0 27.0 35.0 2.0 35.0 110-111 27.220481109905904 34.0 27.0 35.0 2.0 35.0 112-113 27.16855530056673 34.0 27.0 35.0 2.0 35.0 114-115 27.086756459925446 34.0 27.0 35.0 2.0 35.0 116-117 26.97532795389173 34.0 26.0 35.0 2.0 35.0 118-119 26.87046102908911 34.0 25.5 35.0 2.0 35.0 120-121 26.7359921550704 34.0 25.0 35.0 2.0 35.0 122-123 26.54971495253739 34.0 25.0 35.0 2.0 35.0 124-125 25.74582746376244 33.0 22.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6747102514819403 1101 2 0.7104615824740321 1101 3 0.5858838737197836 1101 4 0.6343128661584387 1101 5 0.9610026592721752 1101 6 1.0112131335912125 1101 7 0.997516661407488 1101 8 1.0172285869810977 1101 9 1.0889473732056132 1101 10-11 1.1804549993295197 1101 12-13 1.1292047306997546 1101 14-15 1.214139556863799 1101 16-17 1.306217466764771 1101 18-19 1.2711791323444785 1101 20-21 1.3387587947402082 1101 22-23 1.1823211336597481 1101 24-25 1.1882874275268875 1101 26-27 1.1632655544953252 1101 28-29 1.2403314322347754 1101 30-31 1.3702353728924521 1101 32-33 1.394236658289394 1101 34-35 1.2700849148478142 1101 36-37 1.2352016120492664 1101 38-39 1.3030632767963368 1101 40-41 1.368323211933415 1101 42-43 1.390170040974354 1101 44-45 1.297366445795113 1101 46-47 1.3477710618517236 1101 48-49 1.3572971287408393 1101 50-51 1.3967829245034977 1101 52-53 1.4795658920044872 1101 54-55 1.4095316171214591 1101 56-57 1.3745412225254618 1101 58-59 1.2777892862914406 1101 60-61 1.3379027471786564 1101 62-63 1.4191445365074529 1101 64-65 1.4260772906875125 1101 66-67 1.2729415609621846 1101 68-69 1.347781855075148 1101 70-71 1.2806406948560678 1101 72-73 1.2011905957739124 1101 74-75 1.1801351037242185 1101 76-77 1.2426571693151764 1101 78-79 1.2282203182926494 1101 80-81 1.3068971006337904 1101 82-83 1.28708493136498 1101 84-85 1.3716563769014165 1101 86-87 1.2381628572251344 1101 88-89 1.1995848815039523 1101 90-91 1.3231170818657105 1101 92-93 1.3345722551453676 1101 94-95 1.2258096136233299 1101 96-97 1.2746943546490357 1101 98-99 1.3416283372592943 1101 100-101 1.3005185631236778 1101 102-103 1.23483944341816 1101 104-105 1.3302304175758586 1101 106-107 1.245545770542293 1101 108-109 1.2184699210690866 1101 110-111 1.265655989578427 1101 112-113 1.234547538090034 1101 114-115 1.231763624691908 1101 116-117 1.2099877899904428 1101 118-119 1.2520575324426417 1101 120-121 1.3109006153487144 1101 122-123 1.3129630482601407 1101 124-125 1.0682090474093506 1102 1 0.4378727001083753 1102 2 -0.40688645992268135 1102 3 0.10358367698091087 1102 4 0.47810941138263274 1102 5 0.8563998966875843 1102 6 0.6648656783209788 1102 7 0.7082899172813271 1102 8 0.6928118507862919 1102 9 0.9737397673425932 1102 10-11 1.0854584499851399 1102 12-13 1.158797965354637 1102 14-15 1.237767277473992 1102 16-17 1.343708325102476 1102 18-19 1.3094224396176344 1102 20-21 1.2246157418885204 1102 22-23 1.269708729840339 1102 24-25 1.2356602940431642 1102 26-27 1.3152419561310325 1102 28-29 1.34386603664543 1102 30-31 1.3250474924190385 1102 32-33 1.3049800428160339 1102 34-35 1.170912557174482 1102 36-37 1.1596095928492076 1102 38-39 1.0947371537881878 1102 40-41 1.10567063630225 1102 42-43 1.1010004644264484 1102 44-45 0.9092191903539018 1102 46-47 0.9018219770479945 1102 48-49 1.0525903314642804 1102 50-51 0.8954322760892524 1102 52-53 1.0124787088725355 1102 54-55 1.017960487263501 1102 56-57 0.8078588476503477 1102 58-59 0.8950881439699252 1102 60-61 0.8328047897607824 1102 62-63 0.8862412475691244 1102 64-65 0.8981045766181026 1102 66-67 0.9567126640389354 1102 68-69 0.777289595874258 1102 70-71 0.6391756597829854 1102 72-73 0.6362778407086545 1102 74-75 0.7729016024933131 1102 76-77 0.7857143986962214 1102 78-79 0.7731187557121793 1102 80-81 0.6735194753028892 1102 82-83 0.6797360649311024 1102 84-85 0.7311176111030804 1102 86-87 0.6726632331920896 1102 88-89 0.6949929344071393 1102 90-91 0.6834955724356355 1102 92-93 0.7584103627440193 1102 94-95 0.6294284243316923 1102 96-97 0.5210755439406718 1102 98-99 0.5982999481489486 1102 100-101 0.4955967436496209 1102 102-103 0.581905098579206 1102 104-105 0.589730021007977 1102 106-107 0.8627032543448152 1102 108-109 0.8376045790421607 1102 110-111 0.762968598377082 1102 112-113 0.8023601314379931 1102 114-115 0.8323533262874498 1102 116-117 0.8881538952817927 1102 118-119 0.9317172050938751 1102 120-121 0.9063315867855266 1102 122-123 0.941966838831096 1102 124-125 0.7453080245732195 1103 1 0.15763375770333 1103 2 -1.0031090871320316 1103 3 -0.09667175418574203 1103 4 0.26151361746322266 1103 5 0.5989163131300614 1103 6 0.573935365034508 1103 7 0.4855203838843565 1103 8 0.6009147753719049 1103 9 0.7199811975257049 1103 10-11 0.731896877477979 1103 12-13 0.7651660986532605 1103 14-15 0.870068182909506 1103 16-17 0.9151337351519473 1103 18-19 0.8619074695577567 1103 20-21 0.8253577361608464 1103 22-23 0.7631094646026995 1103 24-25 0.6990974776469727 1103 26-27 0.8062045237494218 1103 28-29 1.0013756550897028 1103 30-31 0.9344977223938713 1103 32-33 0.8838721137466408 1103 34-35 0.9293119901925024 1103 36-37 1.0396778621077942 1103 38-39 0.9975834901010643 1103 40-41 1.0499982130328505 1103 42-43 1.0830407317179578 1103 44-45 1.060006311763253 1103 46-47 1.0059020777162218 1103 48-49 1.0726889747574049 1103 50-51 1.2243360627188906 1103 52-53 1.139148702219174 1103 54-55 1.1157567787385219 1103 56-57 1.1369246291308706 1103 58-59 0.9812088012061828 1103 60-61 0.9490527286655812 1103 62-63 1.0447818475286255 1103 64-65 0.9375724524606177 1103 66-67 0.9536686072250262 1103 68-69 0.9632012508252252 1103 70-71 0.9611534433462978 1103 72-73 0.8925737995156773 1103 74-75 0.8449919146226783 1103 76-77 0.7962147024365684 1103 78-79 0.7908327480174222 1103 80-81 0.8782908022142877 1103 82-83 0.8494653021089213 1103 84-85 0.7973126124048271 1103 86-87 0.8233635716456149 1103 88-89 0.8531178440082279 1103 90-91 0.8659395251099973 1103 92-93 0.8860048838467414 1103 94-95 0.866071804238544 1103 96-97 0.8192758100750694 1103 98-99 0.858869533615696 1103 100-101 0.8822002033967955 1103 102-103 0.7967413680692133 1103 104-105 0.8347964099691829 1103 106-107 0.8442815470347966 1103 108-109 0.8490029417370621 1103 110-111 0.884629045598583 1103 112-113 0.9115409003349804 1103 114-115 0.8165741599823768 1103 116-117 0.8853687409527673 1103 118-119 0.9440288137606032 1103 120-121 0.858366791737943 1103 122-123 0.8178051621843885 1103 124-125 0.7253369522251596 1104 1 0.3788940941732477 1104 2 -0.5248712770379349 1104 3 0.15620962591120602 1104 4 0.3877539938533019 1104 5 0.6677629056592878 1104 6 0.6774640220062764 1104 7 0.7262574811184592 1104 8 0.7443749977842202 1104 9 0.8346817963280984 1104 10-11 0.9571613485358696 1104 12-13 0.9436633897852857 1104 14-15 0.9659229020426636 1104 16-17 1.0980250952888113 1104 18-19 0.9912081539033508 1104 20-21 1.0672917253253829 1104 22-23 1.1128678050646315 1104 24-25 1.0695957667829887 1104 26-27 0.8939910934671218 1104 28-29 1.2238229034501202 1104 30-31 1.175896353702683 1104 32-33 1.2469865990617208 1104 34-35 1.147725876705195 1104 36-37 1.1588245686946124 1104 38-39 1.1848596520628547 1104 40-41 1.1570077653567736 1104 42-43 1.2037799675320429 1104 44-45 1.1650162918031768 1104 46-47 1.2313768424724216 1104 48-49 1.1963808766678738 1104 50-51 1.2488363478628912 1104 52-53 1.017042200936018 1104 54-55 1.2300895417838618 1104 56-57 1.287729448064436 1104 58-59 1.1610284476276256 1104 60-61 1.2895232162618164 1104 62-63 1.2006080522620124 1104 64-65 1.2624869092613054 1104 66-67 1.2190763631417632 1104 68-69 1.1657989126444441 1104 70-71 1.181525556263317 1104 72-73 1.152771974594092 1104 74-75 1.1909364541151213 1104 76-77 1.1711077734374236 1104 78-79 1.170430694980638 1104 80-81 1.0549325187811505 1104 82-83 1.1044617378089505 1104 84-85 1.118426099715915 1104 86-87 1.1726321772914687 1104 88-89 1.1512023892035543 1104 90-91 1.1097861176392279 1104 92-93 1.1578996657115823 1104 94-95 0.9868887417920078 1104 96-97 1.0067501471152767 1104 98-99 1.1186243097776583 1104 100-101 1.1068665849194623 1104 102-103 1.1236839115536732 1104 104-105 1.039196894713978 1104 106-107 1.1129948347450913 1104 108-109 1.0647664147909524 1104 110-111 1.0465124217035147 1104 112-113 1.070351849864494 1104 114-115 1.0696480122777885 1104 116-117 1.088067628891178 1104 118-119 1.0637686198626852 1104 120-121 1.0067215108711025 1104 122-123 0.9989906483549085 1104 124-125 0.9680443944835027 1105 1 0.7540690235564895 1105 2 0.607008351973878 1105 3 0.8767975799157881 1105 4 0.7361275842983517 1105 5 0.9310102756021266 1105 6 0.7870580034568704 1105 7 0.9002168014182743 1105 8 0.9479584367646723 1105 9 1.0443291573743423 1105 10-11 1.1600132090371886 1105 12-13 1.130118762219169 1105 14-15 1.1985605769164422 1105 16-17 1.3851512713080325 1105 18-19 1.3061879697437462 1105 20-21 1.311533679463416 1105 22-23 1.2419221521654507 1105 24-25 1.3311145830904536 1105 26-27 1.3632179233863866 1105 28-29 1.4470159485336396 1105 30-31 1.3801123408915856 1105 32-33 1.3079837410292043 1105 34-35 1.3396828505269553 1105 36-37 1.292821532730116 1105 38-39 1.2987736553101143 1105 40-41 1.209191046422589 1105 42-43 1.212384146709553 1105 44-45 1.2201229397869824 1105 46-47 1.2865313096773363 1105 48-49 1.2338206022077856 1105 50-51 1.2005007347759928 1105 52-53 1.2372789060804763 1105 54-55 1.230321409402464 1105 56-57 1.2139651300999752 1105 58-59 1.189097162736278 1105 60-61 1.186642052586631 1105 62-63 1.2168864379740754 1105 64-65 1.2554945158536874 1105 66-67 1.1203733761085566 1105 68-69 1.0557457554637786 1105 70-71 1.1222345040180386 1105 72-73 1.1093982729415508 1105 74-75 1.1194568940519538 1105 76-77 1.1205953293191868 1105 78-79 1.0835026907102687 1105 80-81 0.8947157197775688 1105 82-83 1.0471943838799902 1105 84-85 0.9393994988840824 1105 86-87 0.9703024915638352 1105 88-89 0.9533391855381552 1105 90-91 0.9006163927665547 1105 92-93 0.707929358111663 1105 94-95 0.7839992375550011 1105 96-97 0.8823021254965369 1105 98-99 0.9147153664712349 1105 100-101 0.9269463206904653 1105 102-103 0.5909541568774017 1105 104-105 0.5651427700281424 1105 106-107 0.8613133218783844 1105 108-109 0.8294476385181824 1105 110-111 0.8252585572432061 1105 112-113 0.7237374507185663 1105 114-115 0.8581845216970656 1105 116-117 0.662140158416765 1105 118-119 0.5998491674483688 1105 120-121 0.5672748793460016 1105 122-123 0.6193255497138637 1105 124-125 0.6019014038916559 1106 1 0.3999493287670859 1106 2 0.43641286161753 1106 3 0.49714274916050627 1106 4 0.3869464833490994 1106 5 0.6042155492167112 1106 6 0.6588820892331668 1106 7 0.6018343368607546 1106 8 0.6115152288766623 1106 9 0.6868325316222155 1106 10-11 0.813515515202532 1106 12-13 0.6676145014799602 1106 14-15 0.7804670634572304 1106 16-17 0.9643105469694859 1106 18-19 0.8954518513823473 1106 20-21 0.9542522908015698 1106 22-23 0.7552128680898349 1106 24-25 0.8604157510266859 1106 26-27 0.7727259936772128 1106 28-29 0.9905556590523688 1106 30-31 0.8211865322741119 1106 32-33 0.8148859775631223 1106 34-35 0.8553816890301249 1106 36-37 0.7632363334004992 1106 38-39 0.8015274129171956 1106 40-41 0.945838297569658 1106 42-43 0.8431836352491331 1106 44-45 0.8993070393521876 1106 46-47 0.7268206449934311 1106 48-49 0.9105525765978371 1106 50-51 1.0071394816163917 1106 52-53 0.9832482145914838 1106 54-55 1.0130038012376374 1106 56-57 0.836500018792723 1106 58-59 0.8260518632438654 1106 60-61 0.992570747774419 1106 62-63 0.9804822206793808 1106 64-65 0.8561469670344124 1106 66-67 0.7301219688840597 1106 68-69 0.8315661570422037 1106 70-71 0.8783697001708823 1106 72-73 0.9074113303363909 1106 74-75 0.9886423802355715 1106 76-77 1.116973057050867 1106 78-79 0.9822124701907242 1106 80-81 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-2.6940527057273975 2216 72-73 -3.1548232205236637 2216 74-75 -2.7367857394554456 2216 76-77 -3.0288522575063617 2216 78-79 -2.6454080105376683 2216 80-81 -2.345828612386118 2216 82-83 -2.2549827782646297 2216 84-85 -2.2101386673461434 2216 86-87 -2.3288789108983217 2216 88-89 -2.095014303249606 2216 90-91 -2.020360005792096 2216 92-93 -2.1256762754952234 2216 94-95 -2.02874947378303 2216 96-97 -2.0900806935242286 2216 98-99 -1.9615383599833898 2216 100-101 -2.2705083506143175 2216 102-103 -2.00949255397839 2216 104-105 -2.042406809944783 2216 106-107 -2.1596659010293635 2216 108-109 -2.2178960400826924 2216 110-111 -2.039054021480254 2216 112-113 -2.178855860295382 2216 114-115 -2.248715405291403 2216 116-117 -2.309656713553384 2216 118-119 -2.404534844122189 2216 120-121 -2.489312957067696 2216 122-123 -2.5148923669450767 2216 124-125 -2.4512928927779747 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 101959.0 3 32652.0 4 15423.0 5 11818.0 6 12726.0 7 15147.0 8 17248.0 9 18714.0 10 20106.0 11 22401.0 12 25324.0 13 28122.0 14 30692.0 15 25444.0 16 17414.0 17 14599.0 18 13630.0 19 12439.0 20 11992.0 21 12804.0 22 15183.0 23 19971.0 24 26657.0 25 35436.0 26 43410.0 27 49015.0 28 56875.0 29 65911.0 30 76995.0 31 91091.0 32 110880.0 33 137182.0 34 177336.0 35 242054.0 36 379635.0 37 576587.0 38 230135.0 39 1194.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.67570532876098 19.989536503610513 13.563754505762619 24.771003661865887 2 15.402209666746236 20.54411728390864 38.85204838490905 25.201624664436075 3 19.43930354076835 24.584856381926766 29.181879271196888 26.793960806108004 4 12.966258064769951 17.103267324926673 36.21894042004222 33.71153419026115 5 13.030106379020696 38.71386418859166 33.90380526085327 14.352224171534383 6 32.086957019311306 37.21751700351837 16.81175715778564 13.88376881938469 7 28.18320740634512 32.68067307131793 20.40117345094069 18.73494607139626 8 26.104561152792666 36.688099317609854 18.64107766215662 18.566261867440858 9 25.913505537377972 16.35399593516724 19.600247337527392 38.132251189927395 10-11 24.962851142807096 27.15868184672001 26.79115641596772 21.08731059450517 12-13 25.86661163377062 24.160097084895813 27.734308426596264 22.238982854737298 14-15 22.503677144479365 25.661291404199773 25.87937177830731 25.95565967301356 16-17 21.91971961262459 28.836138340182593 26.66632077446457 22.57782127272825 18-19 21.810456386186157 27.243624417534466 29.290852517286435 21.655066678992938 20-21 22.672928249910136 26.1736623645107 29.546279621658257 21.607129763920906 22-23 22.128404448672146 26.309268520023725 29.395687279572495 22.16663975173163 24-25 22.017211924321607 26.270625752583594 29.671883387496106 22.040278935598693 26-27 21.802527786807886 26.710240072155038 29.388802879335213 22.09842926170186 28-29 21.68105154816605 26.70072308159388 29.472091576619153 22.14613379362092 30-31 21.896208462839404 26.488510661429558 29.57248066215555 22.04280021357549 32-33 21.70128172023321 26.46712225589628 29.525272112517282 22.306323911353225 34-35 22.008253340872134 26.602218510042736 29.510163968899235 21.879364180185902 36-37 22.144636727328194 26.238641498448693 29.36257923033719 22.254142543885923 38-39 21.918882083226492 26.617381940711702 29.245233085890465 22.218502890171344 40-41 22.303260745561566 26.343867983739365 29.268657725249366 22.084213545449703 42-43 22.01575342147305 26.44685026345709 29.099482098400948 22.437914216668915 44-45 22.12517984222164 26.726082996179457 28.864752569647173 22.28398459195172 46-47 22.35573677772276 26.860941073768164 28.294029091987177 22.4892930565219 48-49 22.379642353589116 26.87939603693945 28.1641944104449 22.576767199026534 50-51 22.446054691949417 27.215725978416692 27.858171936548626 22.480047393085265 52-53 22.5843212377957 27.14061762111497 27.848597192650804 22.42646394843853 54-55 22.483308603351475 27.11039013289817 27.92404766324023 22.48225360051012 56-57 22.458842618636936 27.242050428498825 27.74436485504927 22.554742097814966 58-59 22.42611000176847 27.0129413108967 27.880690243779014 22.680258443555818 60-61 22.36977956877921 27.02268184583297 28.10332662065424 22.50421196473358 62-63 22.341151440829897 26.99904262962498 28.18706523601129 22.472740693533837 64-65 22.235259848210802 27.041691335734587 28.150185583512936 22.572863232541675 66-67 22.264118352006886 27.1628005282882 27.967123965694885 22.60595715401003 68-69 22.563745392155194 26.8742472996666 28.123325283668 22.438682024510204 70-71 22.384406557325455 26.83256675753996 27.976976619348896 22.806050065785684 72-73 22.37768665208942 26.95923806335739 27.881992948845873 22.78108233570732 74-75 22.239381134693687 27.245899176884826 27.797777964546853 22.71694172387463 76-77 22.40511098288036 27.022079630580997 27.857131234120047 22.7156781524186 78-79 22.504954096155064 27.038761220254415 27.828644957693932 22.627639725896593 80-81 22.37775505215459 27.19098362986219 27.789751546630853 22.641509771352375 82-83 22.44233097602151 27.2341846156212 27.820463784069304 22.503020624287984 84-85 22.39355223910488 27.154914597352008 27.716463315959132 22.73506984758398 86-87 22.35922998134606 27.14354021469198 27.745609028278455 22.751620775683502 88-89 22.555656764302707 27.119116258094465 27.641539360010242 22.683687617592586 90-91 22.3552777500616 27.287773661478553 27.754907461945688 22.602041126514152 92-93 22.261097125881907 27.161838558615138 27.876876493984003 22.70018782151895 94-95 22.243895739651702 27.19416646191273 27.800166592812186 22.761771205623383 96-97 22.408641099729508 27.04858991292306 27.733810723979296 22.808958263368137 98-99 22.328190527655405 27.035197948337576 27.626917733765616 23.009693790241407 100-101 22.158035595980454 27.087839098000078 27.83465981256973 22.91946549344974 102-103 22.15807359816322 26.837356803301628 27.903751433866887 23.10081816466827 104-105 22.11560056934818 26.891598538005425 27.80722271098427 23.185578181662127 106-107 22.102068807659364 26.958715046735488 27.667463622854342 23.271752522750806 108-109 22.0737213549588 26.890936177769003 27.622824368114294 23.412518099157904 110-111 22.05741745719726 26.887559252913938 27.719750995155696 23.3352722947331 112-113 22.1312583509054 26.935854317292918 27.68874119131477 23.244146140486908 114-115 21.936150215936838 27.017317862747426 27.578063420628368 23.468468500687365 116-117 21.89267342589104 27.02280062493529 27.67548856374499 23.40903738542868 118-119 21.741629230983264 27.033409402785065 27.63196905436051 23.59299231187116 120-121 21.67041064674551 27.102678050082957 27.710853500482774 23.51605780268876 122-123 21.778022954633443 27.067834813186266 27.55916803982594 23.594974192354357 124-125 21.679790110727605 26.97211978938115 27.61888617204926 23.72920392784198 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 70.0 1 64.5 2 170.0 3 391.5 4 360.0 5 308.0 6 874.0 7 1898.0 8 2823.5 9 3250.5 10 2707.0 11 1946.0 12 1911.0 13 2063.5 14 2244.0 15 2522.0 16 2764.5 17 3123.5 18 3567.5 19 3972.5 20 4520.5 21 5451.0 22 6565.5 23 7992.5 24 9804.5 25 12150.0 26 15852.5 27 20166.5 28 24232.5 29 28778.5 30 33801.0 31 39734.0 32 46694.0 33 52345.0 34 57583.0 35 63098.0 36 68946.5 37 74553.0 38 77788.0 39 80398.5 40 83185.0 41 86562.5 42 89601.0 43 91858.0 44 94890.5 45 97721.5 46 100246.5 47 102389.0 48 104756.0 49 105640.0 50 104405.5 51 101817.5 52 98162.5 53 95092.0 54 92191.0 55 86467.0 56 79463.5 57 72642.0 58 65819.5 59 58767.0 60 49199.5 61 40382.5 62 32548.0 63 24126.5 64 17726.5 65 13789.0 66 10302.5 67 7709.0 68 5841.5 69 4324.5 70 3219.5 71 2234.0 72 1589.0 73 1220.5 74 926.5 75 678.5 76 492.0 77 283.0 78 168.0 79 105.0 80 64.0 81 44.5 82 29.0 83 19.0 84 15.5 85 11.5 86 5.5 87 2.5 88 2.5 89 3.5 90 4.0 91 2.0 92 0.0 93 1.0 94 1.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.061655081304956255 2 0.072383923759415 3 0.0 4 0.008726125196293115 5 0.03265144386973612 6 5.364421227229373E-4 7 1.430512327261166E-4 8 0.0 9 7.867817799936414E-4 10-11 0.06081465531269033 12-13 0.010639435434004923 14-15 0.007635359546756475 16-17 0.05350116103956761 18-19 1.9669544499841034E-4 20-21 0.011301047385363213 22-23 0.012713678308533613 24-25 0.0 26-27 0.0 28-29 0.05101564587095134 30-31 0.0 32-33 0.004112722940875852 34-35 0.0 36-37 8.940702045382289E-5 38-39 0.0 40-41 0.0 42-43 0.0039339088999682074 44-45 0.0 46-47 5.3644212272293727E-5 48-49 1.2516982863535205E-4 50-51 4.4703510226911443E-4 52-53 1.6093263681688118E-4 54-55 0.0 56-57 0.0015735635599872827 58-59 1.7881404090764577E-5 60-61 0.0 62-63 0.0 64-65 7.331375677213476E-4 66-67 0.0 68-69 6.616119513582893E-4 70-71 0.0 72-73 0.002253056915436337 74-75 0.004148485749057382 76-77 9.477144168105226E-4 78-79 0.0010728842454458746 80-81 0.0015199193477149892 82-83 2.6822106136146866E-4 84-85 1.9669544499841034E-4 86-87 3.2186527363376236E-4 88-89 0.0 90-91 0.0 92-93 0.00699162899948895 94-95 0.005793574925407723 96-97 0.006008151774496898 98-99 0.0010550028413551101 100-101 5.185607186321727E-4 102-103 1.2516982863535205E-4 104-105 7.510189718121122E-4 106-107 0.005221369994503257 108-109 0.0052034885904124915 110-111 0.0010907656495366394 112-113 0.003468992393608328 114-115 4.64916506359879E-4 116-117 0.0025570407849793346 118-119 0.002574922189070099 120-121 0.013571985704890314 122-123 0.0011980540740812267 124-125 0.0021457684908917493 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2796201.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.90413857124414 #Duplication Level Percentage of deduplicated Percentage of total 1 79.60217422507242 50.07306198026274 2 12.039522919021874 15.146716360596436 3 3.8075354325344613 7.185292093892093 4 1.6229238754416744 4.083545134054547 5 0.8624264016402552 2.7125094938139043 6 0.5059902096659157 1.909732695871058 7 0.3341972891104951 1.4715674809038508 8 0.2177866453017536 1.0959745052022325 9 0.1574945437736322 0.8916352745176295 >10 0.7722553190091329 8.726181141157134 >50 0.05266150545501098 2.281960150687065 >100 0.02292058951148891 2.6867518668729975 >500 0.0015404919046952412 0.6525182194497753 >1k 4.564420457682001E-4 0.5281948536687456 >5k 1.1411051144205002E-4 0.5543587490499045 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8240 0.2946855394158002 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7206 0.25770679575609906 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4725 0.16897926865772525 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.576280818152915E-5 0.0 0.0 0.0 0.0 3 3.576280818152915E-5 0.0 0.0 0.0 0.0 4 3.576280818152915E-5 0.0 0.0 0.0 0.0 5 3.576280818152915E-5 0.0 0.0 0.0 0.0 6 3.576280818152915E-5 0.0 0.0 0.0 0.0 7 3.576280818152915E-5 0.0 0.0 0.0 0.0 8 3.576280818152915E-5 0.0 0.0 0.0 0.0 9 3.576280818152915E-5 0.0 0.0 0.0 0.0 10-11 3.576280818152915E-5 0.0 0.0 0.0 0.0 12-13 7.15256163630583E-5 0.0 0.0 0.0 0.0 14-15 1.430512327261166E-4 0.0 0.0 3.576280818152915E-5 3.576280818152915E-5 16-17 1.430512327261166E-4 0.0 0.0 5.3644212272293727E-5 3.576280818152915E-5 18-19 1.9669544499841034E-4 0.0 0.0 1.2516982863535205E-4 7.15256163630583E-5 20-21 2.503396572707041E-4 3.576280818152915E-5 0.0 2.1457684908917493E-4 7.15256163630583E-5 22-23 2.503396572707041E-4 3.576280818152915E-5 0.0 3.5762808181529155E-4 8.940702045382289E-5 24-25 2.861024654522332E-4 3.576280818152915E-5 0.0 6.079677390859956E-4 1.0728842454458747E-4 26-27 2.861024654522332E-4 3.576280818152915E-5 0.0 7.331375677213476E-4 1.0728842454458747E-4 28-29 2.861024654522332E-4 3.576280818152915E-5 0.0 0.0011622912658996974 1.0728842454458747E-4 30-31 2.861024654522332E-4 3.576280818152915E-5 0.0 0.0021278870868009846 1.0728842454458747E-4 32-33 2.861024654522332E-4 3.576280818152915E-5 0.0 0.0050604373576863745 1.0728842454458747E-4 34-35 2.861024654522332E-4 3.576280818152915E-5 0.0 0.008636718175839291 1.0728842454458747E-4 36-37 2.861024654522332E-4 5.3644212272293727E-5 0.0 0.014823683991243834 1.430512327261166E-4 38-39 3.2186527363376236E-4 8.940702045382289E-5 0.0 0.0253915938088857 1.430512327261166E-4 40-41 3.397466777245269E-4 1.0728842454458747E-4 0.0 0.03989341252649577 1.430512327261166E-4 42-43 4.112722940875853E-4 1.0728842454458747E-4 0.0 0.05283954908820933 1.609326368168812E-4 44-45 4.64916506359879E-4 1.2516982863535205E-4 0.0 0.06846789626353755 1.7881404090764577E-4 46-47 4.64916506359879E-4 1.430512327261166E-4 0.0 0.08790498251019865 1.7881404090764577E-4 48-49 4.64916506359879E-4 1.430512327261166E-4 0.0 0.10961300707638685 1.7881404090764577E-4 50-51 4.64916506359879E-4 1.430512327261166E-4 0.0 0.13369925838664676 1.7881404090764577E-4 52-53 4.8279791045064357E-4 1.430512327261166E-4 0.0 0.15853652866871873 1.9669544499841034E-4 54-55 5.364421227229373E-4 1.9669544499841034E-4 0.0 0.18587719552349777 2.1457684908917493E-4 56-57 5.364421227229373E-4 2.1457684908917493E-4 0.0 0.21829618114005395 2.1457684908917493E-4 58-59 5.364421227229373E-4 3.2186527363376236E-4 0.0 0.2535404286029509 2.1457684908917493E-4 60-61 5.543235268137018E-4 3.5762808181529155E-4 0.0 0.2947391836280725 2.1457684908917493E-4 62-63 5.722049309044665E-4 3.5762808181529155E-4 0.0 0.33661743200864314 2.503396572707041E-4 64-65 5.722049309044665E-4 3.5762808181529155E-4 0.0 0.3801407695655641 2.6822106136146866E-4 66-67 5.722049309044665E-4 3.5762808181529155E-4 0.0 0.4310848898201524 2.861024654522332E-4 68-69 5.722049309044665E-4 3.5762808181529155E-4 0.0 0.4918637823246612 2.861024654522332E-4 70-71 5.722049309044665E-4 3.933908899968207E-4 0.0 0.5629244821813596 2.861024654522332E-4 72-73 5.722049309044665E-4 4.2915369817834987E-4 0.0 0.636649511247582 3.5762808181529155E-4 74-75 5.722049309044665E-4 4.2915369817834987E-4 0.0 0.7106069985669843 3.5762808181529155E-4 76-77 5.722049309044665E-4 4.2915369817834987E-4 0.0 0.7942919697117625 3.5762808181529155E-4 78-79 5.722049309044665E-4 4.2915369817834987E-4 0.0 0.8916740963900665 3.5762808181529155E-4 80-81 6.79493355449054E-4 4.2915369817834987E-4 0.0 1.0074919506859485 3.5762808181529155E-4 82-83 6.79493355449054E-4 4.2915369817834987E-4 0.0 1.1323935582599391 3.933908899968207E-4 84-85 6.79493355449054E-4 4.2915369817834987E-4 0.0 1.2538976990566844 3.933908899968207E-4 86-87 6.79493355449054E-4 4.2915369817834987E-4 0.0 1.3897963701464953 3.933908899968207E-4 88-89 6.79493355449054E-4 4.2915369817834987E-4 0.0 1.5461513675161407 4.64916506359879E-4 90-91 8.225445881751705E-4 4.2915369817834987E-4 0.0 1.7238746427742497 4.64916506359879E-4 92-93 8.225445881751705E-4 4.2915369817834987E-4 0.0 1.9173335536322318 5.006793145414082E-4 94-95 8.404259922659351E-4 4.2915369817834987E-4 0.0 2.111918992947932 5.722049309044665E-4 96-97 8.583073963566997E-4 4.64916506359879E-4 0.0 2.3140146219817535 5.722049309044665E-4 98-99 9.655958209012871E-4 4.64916506359879E-4 0.0 2.542181338179909 5.722049309044665E-4 100-101 0.0010013586290828162 4.64916506359879E-4 0.0 2.807273153825494 5.722049309044665E-4 102-103 0.0010371214372643454 5.364421227229373E-4 0.0 3.0924457862650074 6.258491431767601E-4 104-105 0.0011265284577181684 5.364421227229373E-4 0.0 3.3797999500035942 6.616119513582893E-4 106-107 0.0011980540740812267 6.794933554490539E-4 0.0 3.6652944477167413 6.79493355449054E-4 108-109 0.0012159354781719912 7.152561636305831E-4 0.0 3.976252064855137 6.79493355449054E-4 110-111 0.0012159354781719912 7.152561636305831E-4 0.0 4.334631165642241 6.973747595398186E-4 112-113 0.0012159354781719912 7.152561636305831E-4 0.0 4.729989010089046 7.152561636305831E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7600 0.0 47.10653 1 GTATCAA 11630 0.0 43.02489 1 TATCAAC 14430 0.0 34.5327 2 ATCAACG 14520 0.0 34.2984 3 TCAACGC 14535 0.0 34.019825 4 CAACGCA 14720 0.0 32.98029 5 AACGCAG 15245 0.0 32.031292 6 GTACATG 13345 0.0 29.951883 1 TACATGG 13120 0.0 29.767437 2 ACATGGG 13440 0.0 27.97904 3 TAGGACG 725 0.0 27.90532 4 GTATCGC 150 5.262851E-6 27.790401 6 ACGCAGA 18045 0.0 26.929548 7 CGCAGAG 18105 0.0 26.807411 8 TACCTGG 2750 0.0 24.03044 2 GTACCTG 2910 0.0 23.950418 1 GAGTACT 11220 0.0 23.59254 12-13 GCAGAGT 20055 0.0 23.37067 9 GTACTTT 11625 0.0 22.821833 14-15 AGTACTT 11770 0.0 22.363594 12-13 >>END_MODULE