FastQCFastQC Report
Thu 26 May 2016
SRR1364623_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364623_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3361182
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44950.1337327166455134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG199250.035.487981
TACATGG202250.033.7819182
GTATCAA153750.032.23571
ACATGGG206700.031.6425363
TCAACGC176350.027.0561814
ATCAACG176950.027.0317653
CAACGCA178250.026.8696425
CGCATAG4050.026.4440638
AACGCAG178450.026.182996
TATCAAC188750.026.1308062
AACGCAT4800.026.040196
CATGGGA143950.026.0284584
CATGGGG71600.024.708874
GGACCGA5400.024.2490676
ATGGGAT55600.023.4641425
GGTATCA113100.023.227631
ATGGGAG56000.022.8710335
ACGCAGA206150.022.170697
GTTATCA21050.022.073511
ACGCATA4900.021.861397
Produced by FastQC (version 0.11.5)