##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364623_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3361182 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.84445709872301 31.0 27.0 31.0 10.0 34.0 2 26.58851737275756 31.0 26.0 31.0 10.0 34.0 3 27.146429738109987 31.0 27.0 31.0 10.0 34.0 4 30.472853002306927 35.0 30.0 37.0 10.0 37.0 5 30.848338173892397 35.0 31.0 37.0 12.0 37.0 6 31.123874279940807 35.0 32.0 37.0 11.0 37.0 7 31.05193173115886 35.0 32.0 37.0 10.0 37.0 8 30.742950247859234 35.0 31.0 37.0 10.0 37.0 9 31.76448017393881 37.0 31.0 39.0 8.0 39.0 10-11 31.729052309574428 37.0 31.0 39.0 8.0 39.0 12-13 31.711122753840762 37.0 31.0 39.0 8.0 39.0 14-15 32.411693118670755 38.0 30.5 40.0 8.0 41.0 16-17 32.83179815909998 38.0 32.0 40.0 8.0 41.0 18-19 32.68424232903782 38.0 31.0 40.0 8.0 41.0 20-21 32.63067531005462 38.0 31.0 40.0 8.0 41.0 22-23 32.728817273209245 38.0 31.0 40.0 8.0 41.0 24-25 32.84353986187002 38.0 31.0 40.0 8.0 41.0 26-27 32.672711117696096 38.0 31.0 40.0 8.0 41.0 28-29 32.59200870408088 38.0 31.0 40.0 8.0 41.0 30-31 32.46879713743559 38.0 30.0 40.0 4.5 41.0 32-33 32.37148598320472 38.0 30.0 40.0 2.0 41.0 34-35 32.42336490556001 38.0 30.0 40.0 2.0 41.0 36-37 32.357946400998216 38.0 30.0 40.0 2.0 41.0 38-39 32.06218467193982 38.0 30.0 40.0 2.0 41.0 40-41 31.92933453172128 38.0 30.0 40.0 2.0 41.0 42-43 31.432926720421566 37.5 29.5 40.0 2.0 41.0 44-45 31.326273911975015 37.0 30.0 40.0 2.0 41.0 46-47 31.295673962314446 37.0 29.5 40.0 2.0 41.0 48-49 31.229624727253686 37.0 29.0 40.0 2.0 41.0 50-51 30.99396254055865 37.0 29.0 40.0 2.0 41.0 52-53 30.89710628582445 37.0 29.0 40.0 2.0 41.0 54-55 30.728767290792348 36.0 29.0 40.0 2.0 41.0 56-57 30.506949341035387 36.0 28.5 40.0 2.0 41.0 58-59 30.29821800783177 36.0 28.0 40.0 2.0 41.0 60-61 30.000384388587108 35.0 28.0 39.0 2.0 41.0 62-63 29.831025365481548 35.0 28.0 39.0 2.0 41.0 64-65 29.601765985894247 35.0 28.0 39.0 2.0 40.0 66-67 29.322339135458897 34.5 28.0 38.5 2.0 40.0 68-69 29.08140425005251 34.0 27.5 38.0 2.0 40.0 70-71 28.62577733071283 34.0 26.0 37.5 2.0 40.0 72-73 28.385801482930706 34.0 26.0 37.0 2.0 39.0 74-75 28.336132199922528 34.0 27.0 36.5 2.0 39.0 76-77 28.063968568200117 34.0 26.0 36.0 2.0 39.0 78-79 27.75838663303564 34.0 26.0 36.0 2.0 38.5 80-81 27.33399069136988 33.5 26.0 35.0 2.0 37.0 82-83 27.440332151011162 34.0 26.0 35.0 2.0 37.0 84-85 27.259919427153903 34.0 26.0 35.0 2.0 36.5 86-87 27.123959964084065 34.0 26.0 35.0 2.0 36.0 88-89 26.95728303317107 34.0 26.0 35.0 2.0 36.0 90-91 26.828887427101538 34.0 26.0 35.0 2.0 36.0 92-93 26.702455564738834 33.5 25.5 35.0 2.0 35.0 94-95 26.598044080921532 33.0 25.0 35.0 2.0 35.0 96-97 26.484682918092503 33.0 25.0 35.0 2.0 35.0 98-99 26.38774469814488 33.0 25.0 35.0 2.0 35.0 100-101 26.28279039932976 33.0 25.0 35.0 2.0 35.0 102-103 25.127491906121122 31.5 21.5 34.0 2.0 35.0 104-105 25.97964599953231 33.0 24.0 35.0 2.0 35.0 106-107 26.134869519115597 33.0 24.0 35.0 2.0 35.0 108-109 26.091924061237982 33.0 24.0 35.0 2.0 35.0 110-111 26.02536920047769 33.0 24.0 35.0 2.0 35.0 112-113 25.93578836254627 33.0 24.0 35.0 2.0 35.0 114-115 25.82953228953386 33.0 23.0 35.0 2.0 35.0 116-117 25.721395628085595 33.0 23.0 35.0 2.0 35.0 118-119 25.59739445825903 33.0 21.0 35.0 2.0 35.0 120-121 25.418835992814433 33.0 19.0 35.0 2.0 35.0 122-123 25.213473415006984 33.0 16.5 35.0 2.0 35.0 124-125 24.265419724370773 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7152842625202567 1101 2 1.9640969608606902 1101 3 1.5469626939677887 1101 4 1.730588468985708 1101 5 1.624948879963675 1101 6 1.5230820925148514 1101 7 1.6411633872893674 1101 8 1.7684382548874282 1101 9 2.2053501162351345 1101 10-11 2.1372136816094773 1101 12-13 2.133267340067981 1101 14-15 2.4929420218009284 1101 16-17 2.002838121013852 1101 18-19 2.1227026397398845 1101 20-21 2.335148684404011 1101 22-23 2.1259363094320207 1101 24-25 2.000721176667355 1101 26-27 2.2641766248349597 1101 28-29 2.1877625116669392 1101 30-31 2.3358082529885635 1101 32-33 2.3532459526323706 1101 34-35 2.184372336809311 1101 36-37 2.0199635079336176 1101 38-39 2.047853947736236 1101 40-41 2.170002104272445 1101 42-43 2.287958344587434 1101 44-45 2.1375685201605457 1101 46-47 1.8029095752549829 1101 48-49 1.7443902647453626 1101 50-51 1.8158848621183843 1101 52-53 1.7045220260079361 1101 54-55 1.6996846695509618 1101 56-57 1.753993670061412 1101 58-59 1.6741103844963554 1101 60-61 1.6366865371371837 1101 62-63 1.6603020244295408 1101 64-65 1.6875451969904418 1101 66-67 1.6632339906614142 1101 68-69 1.5710015167749312 1101 70-71 1.4356220532277568 1101 72-73 1.4323312196851887 1101 74-75 1.4667689269718274 1101 76-77 1.4831080713397817 1101 78-79 1.4975237875078022 1101 80-81 1.6420811263710355 1101 82-83 1.677172247448997 1101 84-85 1.682394438894562 1101 86-87 1.5836798093783067 1101 88-89 1.5991471441666611 1101 90-91 1.622057523331101 1101 92-93 1.5956188474640705 1101 94-95 1.573212376100745 1101 96-97 1.5747605750593863 1101 98-99 1.6041215362650725 1101 100-101 1.6605810987553014 1101 102-103 1.3719009857651017 1101 104-105 1.5581851676645968 1101 106-107 1.6220305451854422 1101 108-109 1.6151391517854954 1101 110-111 1.6622861651674583 1101 112-113 1.5780458593136828 1101 114-115 1.640598891847013 1101 116-117 1.5750664948846556 1101 118-119 1.4894637020025456 1101 120-121 1.6096568459720721 1101 122-123 1.5818929436254798 1101 124-125 1.3979652336574375 1102 1 0.8678072536253367 1102 2 1.2572915937568148 1102 3 0.9625966454226358 1102 4 0.9004422304812572 1102 5 0.816645698899304 1102 6 0.7716800120082894 1102 7 0.8339441802940399 1102 8 0.8019133038848629 1102 9 1.3431731224163492 1102 10-11 1.3679015302450885 1102 12-13 1.5040806975231291 1102 14-15 1.6192875668031945 1102 16-17 1.4207391461909964 1102 18-19 1.214426595717569 1102 20-21 1.5493775401254055 1102 22-23 1.5000068657413834 1102 24-25 1.2379976729737052 1102 26-27 1.5816332809483384 1102 28-29 1.2662149464626609 1102 30-31 1.4263374253087804 1102 32-33 1.6389910905789975 1102 34-35 1.4763676632076184 1102 36-37 1.3003818706654116 1102 38-39 1.5976485354039625 1102 40-41 1.6498788568730767 1102 42-43 1.9038791195007398 1102 44-45 1.4614200956165995 1102 46-47 1.3033166308859272 1102 48-49 1.2431020090541267 1102 50-51 1.2721795251667807 1102 52-53 1.1899818481095465 1102 54-55 1.2182290687670019 1102 56-57 1.2589654023092613 1102 58-59 1.2315135203323244 1102 60-61 1.226077838207587 1102 62-63 1.028801195242142 1102 64-65 0.9025828873266377 1102 66-67 0.983929000460904 1102 68-69 0.7587917323636582 1102 70-71 1.0044231236333054 1102 72-73 0.6293593366759893 1102 74-75 0.8290506999252436 1102 76-77 0.7492919247997527 1102 78-79 0.7400742925583117 1102 80-81 0.8828507630361955 1102 82-83 0.8533142646357881 1102 84-85 0.8190927654436351 1102 86-87 0.8602367971666389 1102 88-89 0.7205394251858088 1102 90-91 0.8317032341708384 1102 92-93 0.9127958412828541 1102 94-95 0.9013591422699001 1102 96-97 0.7370732540884859 1102 98-99 0.8568608698999292 1102 100-101 0.9217871895136298 1102 102-103 0.7681752960319486 1102 104-105 0.81705445757866 1102 106-107 0.758997603831606 1102 108-109 0.8440171104768872 1102 110-111 0.7615519574183232 1102 112-113 0.6907980076628419 1102 114-115 0.7914122493021623 1102 116-117 0.8812220805924795 1102 118-119 0.9134049804587505 1102 120-121 0.9269359806019537 1102 122-123 0.7340703143476262 1102 124-125 0.8156137335820581 1103 1 1.0661047165032933 1103 2 1.3088185644241648 1103 3 0.9454306699413308 1103 4 0.7780807638722074 1103 5 0.8937234857093301 1103 6 0.9349603404732711 1103 7 0.9223827074396453 1103 8 1.016292746349933 1103 9 1.4036012241967093 1103 10-11 1.436267246208697 1103 12-13 1.3490962303066638 1103 14-15 1.671449816901152 1103 16-17 1.3539325845804768 1103 18-19 1.375445210210735 1103 20-21 1.6312513592594726 1103 22-23 1.4786869787083106 1103 24-25 1.2617459005205802 1103 26-27 1.5613393433339837 1103 28-29 1.609294085759764 1103 30-31 1.7045527131806892 1103 32-33 1.7749748226240385 1103 34-35 1.5346616441364844 1103 36-37 1.4095459695205363 1103 38-39 1.531987038105406 1103 40-41 1.5987634369758759 1103 42-43 1.7979689180278449 1103 44-45 1.5767485161111416 1103 46-47 1.4562109633002471 1103 48-49 1.296549046325083 1103 50-51 1.2515978944461246 1103 52-53 1.171456628154118 1103 54-55 1.1801937694587252 1103 56-57 1.205178795255442 1103 58-59 1.2884297473903246 1103 60-61 1.2690151686640299 1103 62-63 1.2134135404809214 1103 64-65 0.9432782513649229 1103 66-67 0.8743538754784055 1103 68-69 1.0067082500317746 1103 70-71 1.048920741671214 1103 72-73 1.0188455838164785 1103 74-75 1.0424101948848126 1103 76-77 1.0563689653579154 1103 78-79 1.008165842804651 1103 80-81 1.0835008919556088 1103 82-83 1.1027914686138764 1103 84-85 1.1657376707678608 1103 86-87 1.0270889565291057 1103 88-89 1.0071928574239593 1103 90-91 1.0329146469053718 1103 92-93 0.9758660859963868 1103 94-95 0.9863981312018666 1103 96-97 1.0087472120304355 1103 98-99 1.047772747711388 1103 100-101 0.9236125266196922 1103 102-103 0.8750021533428836 1103 104-105 0.9842275514129923 1103 106-107 1.0669739660308686 1103 108-109 1.0945967567887571 1103 110-111 1.048356444801211 1103 112-113 1.057370733894679 1103 114-115 1.1473555452406359 1103 116-117 1.0824355079552284 1103 118-119 1.1068329400399328 1103 120-121 1.1068368869160317 1103 122-123 1.0049545170434016 1103 124-125 0.9348294211897787 1104 1 1.2655766147886176 1104 2 1.6291800339169278 1104 3 1.075858687856666 1104 4 1.2860320694547163 1104 5 0.9112748351857505 1104 6 1.1259442164991533 1104 7 0.9008467835614979 1104 8 1.244620652147276 1104 9 1.626870175539299 1104 10-11 1.673349855300028 1104 12-13 1.5159919432865863 1104 14-15 1.6415522345196933 1104 16-17 1.3318540374459147 1104 18-19 1.39800179925534 1104 20-21 1.7496358204524398 1104 22-23 1.6280579938509234 1104 24-25 1.4682632573645407 1104 26-27 1.6133567365847057 1104 28-29 1.6515167443782843 1104 30-31 1.757847018361339 1104 32-33 1.770449847650859 1104 34-35 1.649293986729937 1104 36-37 1.5159176211331058 1104 38-39 1.652062736169455 1104 40-41 1.6118919726656706 1104 42-43 1.8681152295583026 1104 44-45 1.6086622383959615 1104 46-47 1.4221748032618358 1104 48-49 1.1806204462103551 1104 50-51 1.225670418433726 1104 52-53 1.1449088378137304 1104 54-55 1.2504575128446334 1104 56-57 1.262855473704672 1104 58-59 1.269932213274064 1104 60-61 1.2979666281468916 1104 62-63 1.1581462113690684 1104 64-65 1.0895676372909868 1104 66-67 1.1205674184542858 1104 68-69 0.9316461825832469 1104 70-71 1.0791573040897013 1104 72-73 1.0007787566527497 1104 74-75 0.9919007698979385 1104 76-77 1.011336318437717 1104 78-79 0.9950083916299377 1104 80-81 1.139028748557525 1104 82-83 1.0866260043717517 1104 84-85 1.035158283890567 1104 86-87 0.9649644590874331 1104 88-89 1.0166834340835216 1104 90-91 0.9964670177466957 1104 92-93 0.9431446646930759 1104 94-95 0.9188657383469092 1104 96-97 0.9839302141212798 1104 98-99 0.9566413265649025 1104 100-101 0.9775982103058531 1104 102-103 0.8467723873187509 1104 104-105 0.9791118773308867 1104 106-107 1.0055775008161554 1104 108-109 1.0256212239813323 1104 110-111 0.9898903427431733 1104 112-113 0.9641175335679151 1104 114-115 0.980838120892912 1104 116-117 0.8999433586637373 1104 118-119 0.8058581403892582 1104 120-121 0.8724363999998452 1104 122-123 0.8920915140009136 1104 124-125 0.706531994988989 1105 1 1.0181161357549726 1105 2 1.2944301505777673 1105 3 0.8111304377563862 1105 4 0.9917304575921477 1105 5 0.9820243742429646 1105 6 0.9055250050525494 1105 7 0.9850989514618931 1105 8 0.8903239363465651 1105 9 1.2239754692917693 1105 10-11 1.1964748956455757 1105 12-13 1.190846807730388 1105 14-15 1.4786504089903545 1105 16-17 1.0465722633854426 1105 18-19 1.2016252784492494 1105 20-21 1.1778632710285493 1105 22-23 1.242366847035548 1105 24-25 1.0183872928050306 1105 26-27 1.413020873003859 1105 28-29 1.5509740105419567 1105 30-31 1.4699621138722847 1105 32-33 1.5008071092219595 1105 34-35 1.2498614698458326 1105 36-37 0.9500251432440621 1105 38-39 1.27427036307445 1105 40-41 1.2937929750656885 1105 42-43 1.570246630288036 1105 44-45 1.352983212103677 1105 46-47 0.8677481266456759 1105 48-49 0.98813769162075 1105 50-51 1.0918887397573336 1105 52-53 0.8200404806970027 1105 54-55 0.9650385639451358 1105 56-57 1.0491613420418773 1105 58-59 0.9625625602826524 1105 60-61 0.9042686377643072 1105 62-63 0.8772662161093727 1105 64-65 0.634039643828487 1105 66-67 0.658930768870043 1105 68-69 0.49600809918672795 1105 70-71 0.7492642811946304 1105 72-73 0.6760468116235288 1105 74-75 0.7493067459196006 1105 76-77 0.8362559003406922 1105 78-79 0.7996122072502523 1105 80-81 0.8572697423890361 1105 82-83 0.7978422172895812 1105 84-85 0.783258410362798 1105 86-87 0.8404163692902245 1105 88-89 0.8298575659197418 1105 90-91 0.615021044801729 1105 92-93 0.8100345686570414 1105 94-95 0.7345367573540358 1105 96-97 0.664126868323784 1105 98-99 0.8092945938551956 1105 100-101 0.7935824391737043 1105 102-103 0.6293492839124468 1105 104-105 0.712115585587906 1105 106-107 0.6935404307711579 1105 108-109 0.7088723367153626 1105 110-111 0.7484141511888076 1105 112-113 0.6491367444685494 1105 114-115 0.6943776749385933 1105 116-117 0.7256015851235382 1105 118-119 0.7473037134918457 1105 120-121 0.5235726629092134 1105 122-123 0.5802570347739291 1105 124-125 0.6280253131250966 1106 1 0.3953254522609164 1106 2 -1.5832107657870473 1106 3 0.020941730056428298 1106 4 0.5489205225799871 1106 5 0.5954428592378562 1106 6 0.7324426396479353 1106 7 0.7205574457287973 1106 8 0.7122853290468143 1106 9 1.2638691902992925 1106 10-11 1.4691701942908004 1106 12-13 1.3915837391799002 1106 14-15 1.597732114834983 1106 16-17 1.1202050727944766 1106 18-19 1.1986892590264375 1106 20-21 1.4417341620442912 1106 22-23 1.0710763366934444 1106 24-25 1.196880947623825 1106 26-27 1.3305715106405671 1106 28-29 1.3925058793449026 1106 30-31 1.4214893420167343 1106 32-33 1.593097681470546 1106 34-35 1.3697507526709316 1106 36-37 1.1310269413246914 1106 38-39 1.5380451943211213 1106 40-41 1.5104698935381187 1106 42-43 1.841835001551889 1106 44-45 1.5884799324567602 1106 46-47 1.4226854125912247 1106 48-49 1.2515718626930266 1106 50-51 1.2133309791520084 1106 52-53 1.136330565141165 1106 54-55 1.1663717525197157 1106 56-57 1.1132306479725145 1106 58-59 1.0631143308544502 1106 60-61 1.1924618506732507 1106 62-63 1.1516449171159806 1106 64-65 1.151657564031506 1106 66-67 1.0855804368558317 1106 68-69 1.0363193980032435 1106 70-71 1.1611431451130194 1106 72-73 0.9566608447514042 1106 74-75 0.9758937045055625 1106 76-77 0.8959522467414125 1106 78-79 0.932558578553202 1106 80-81 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78-79 -2.4640551013289524 2216 80-81 -2.3924985426744882 2216 82-83 -2.442927945952416 2216 84-85 -2.3246572755664054 2216 86-87 -2.4485689468924683 2216 88-89 -2.5007543653374604 2216 90-91 -2.3744665163083702 2216 92-93 -2.4424537936207855 2216 94-95 -2.5809279606922644 2216 96-97 -2.5602499812172965 2216 98-99 -2.6163614981040766 2216 100-101 -2.706368337113801 2216 102-103 -2.3401829535767114 2216 104-105 -2.3520589038782873 2216 106-107 -2.3532475062735827 2216 108-109 -2.432058804032792 2216 110-111 -2.2645214429974523 2216 112-113 -2.5927349054994266 2216 114-115 -2.458900175899089 2216 116-117 -2.3042379550855436 2216 118-119 -2.373188868776637 2216 120-121 -2.479951520507104 2216 122-123 -2.327048752711711 2216 124-125 -2.1589890502794695 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 246002.0 3 32584.0 4 20386.0 5 16540.0 6 16834.0 7 18947.0 8 20763.0 9 21540.0 10 23320.0 11 25037.0 12 27301.0 13 28758.0 14 30050.0 15 25510.0 16 20984.0 17 18252.0 18 16227.0 19 15051.0 20 14499.0 21 15466.0 22 18909.0 23 27289.0 24 40950.0 25 55401.0 26 62233.0 27 63286.0 28 72570.0 29 84182.0 30 97476.0 31 113101.0 32 138238.0 33 175164.0 34 233322.0 35 320873.0 36 478875.0 37 568434.0 38 156580.0 39 248.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.145887167648254 19.3505718411604 13.822118107766808 22.68142288342454 2 13.420399998091384 21.008011413520634 41.71732450280567 23.85426408558232 3 19.24445893887445 26.789665100094616 27.92059863719868 26.045277323832256 4 11.646527157906796 17.817796913559622 36.94763528833349 33.5880406402001 5 13.46827733612827 39.93399231591532 32.679891680310085 13.917838667646325 6 31.099533451389284 37.25700903786984 17.861652697838885 13.78180481290199 7 28.433206172303937 33.893259272841654 20.130649385885274 17.54288516896913 8 25.516445164105857 36.47411484428843 19.612306925522198 18.39713306608352 9 26.12298006671257 17.924607042677565 19.694576846146738 36.257836044463126 10-11 23.85232367002597 28.800235953223464 26.915157387801187 20.432282988949375 12-13 24.846521697346436 25.9884512261353 27.35587313483428 21.80915394168398 14-15 22.01494299995246 26.9925949636931 25.491395264657292 25.501066771697147 16-17 21.929333045099074 30.42380119702634 25.686603647454543 21.96026211042004 18-19 21.984863985241006 28.72449606846599 27.93459787183788 21.356042074455125 20-21 23.16171200316148 27.776926742665758 27.478258122525812 21.58310313164695 22-23 22.686462076079607 27.73073823506748 27.280427497854866 22.30237219099805 24-25 22.436572477079533 27.688406248518987 27.64207201791014 22.232949256491338 26-27 22.345204065960775 27.851846218766823 27.53079516462608 22.272154550646324 28-29 21.99262437977739 27.868371254451446 27.975593402172453 22.163410963598707 30-31 22.146957126704077 27.61347740135298 27.8611023795655 22.378463092377434 32-33 22.031446605554684 27.699349789127442 27.593492496323012 22.675711108994857 34-35 22.63531868217977 27.53917914588948 27.49642431031013 22.329077861620622 36-37 22.705416490941676 27.35014264822015 27.416748067496865 22.527692793341306 38-39 22.38004577291947 27.475807381784833 27.712793557814763 22.43135328748094 40-41 22.375347468047842 27.443887170697174 27.899392629578568 22.281372731676417 42-43 22.33637775714155 27.41411810399526 27.67967117001153 22.569832968851657 44-45 22.40664549582779 27.75833417393871 27.295720600950645 22.539299729282853 46-47 22.494128408521238 27.760363088937574 26.933967780549594 22.811540721991598 48-49 22.54446281553187 27.699628648447806 27.07033034287521 22.68557819314512 50-51 22.606149746881876 27.817322880632954 26.99947525926394 22.57705211322123 52-53 22.808753763699645 27.784504398713356 26.852246894225978 22.55449494336102 54-55 22.42832393963058 27.716630675725266 27.04924493523046 22.805800449413695 56-57 22.553793945231018 28.043491087584123 26.90358809071426 22.4991268764706 58-59 22.610645768193187 27.524850702135677 27.03376537481249 22.830738154858643 60-61 22.59590602327071 27.672466963633923 27.262145613277116 22.469481399818246 62-63 22.545523610904215 27.71748845523611 27.099702070609265 22.63728586325041 64-65 22.36557480439756 27.559976655511797 27.122193023406155 22.952255516684485 66-67 22.357127090782196 28.021061304589107 26.8873152883612 22.734496316267496 68-69 22.368759703685345 27.80569811127409 27.04081237496172 22.784729810078844 70-71 22.29229196577133 27.923867706854566 27.01019558708073 22.77364474029337 72-73 22.514423292416648 27.722503939852455 26.900075096573477 22.86299767115742 74-75 22.319522866470425 27.97314597523941 26.771987838461868 22.935343319828288 76-77 22.626136759021385 27.744233390687633 26.974597487214442 22.65503236307654 78-79 22.547929314204307 27.804498685321416 26.970498529216368 22.677073471257902 80-81 22.34413615880228 28.20584708204536 27.013697813861683 22.43631894529068 82-83 22.534414372809646 28.07521591615861 26.759957840950143 22.6304118700816 84-85 22.620447977535136 27.814285929207138 26.711985863532682 22.853280229725044 86-87 22.633034115429904 27.69715149264038 26.99086136108016 22.678953030849556 88-89 22.607177432258847 27.607092510921333 27.00367426320311 22.782055793616713 90-91 22.453814671154383 27.965739121678045 27.11687412462613 22.463572082541443 92-93 22.44361462844415 27.7561435374781 27.077849151487733 22.722392682590016 94-95 22.316272727326908 27.74936065191751 27.051128774925036 22.883237845830546 96-97 22.562711153335776 27.704315830043697 26.95411950967806 22.77885350694246 98-99 22.338936607016898 27.685800148763168 26.79594731346617 23.17931593075377 100-101 22.167293029263487 27.79920928176427 27.16272340232854 22.8707742866437 102-103 22.46641331532408 27.536007950692593 27.140648735391387 22.85692999859194 104-105 22.3296974073101 27.538148030981922 27.264887606812398 22.86726695489557 106-107 22.183748876743646 27.807634464193498 27.157192139879132 22.851424519183723 108-109 22.310417794766412 27.92753149306903 26.76505310230844 22.996997609856123 110-111 21.998145237688522 27.973759298198686 27.138952436084494 22.889143028028297 112-113 22.21555398827238 27.861943134624596 27.10032988398663 22.822172993116396 114-115 21.966874029730725 28.006686248887796 26.974755315307263 23.05168440607421 116-117 21.862384594260853 27.997079376274385 27.10449614028525 23.036039889179516 118-119 21.945697213925712 28.181320185842512 26.84653936586446 23.026443234367317 120-121 21.871233616253967 28.120495693508918 27.029553684968445 22.978717005268674 122-123 21.957336400029963 28.00415862649163 26.916145535247427 23.122359438230976 124-125 21.988054167861755 28.063070348108365 26.888263818585855 23.06061166544403 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6104.0 1 4565.5 2 2522.0 3 1832.5 4 1452.5 5 1187.0 6 1164.0 7 1329.0 8 1633.5 9 1726.0 10 1623.5 11 1595.0 12 1686.0 13 1861.5 14 2091.5 15 2364.0 16 2567.0 17 2833.0 18 3263.5 19 3690.5 20 4407.5 21 5579.0 22 7028.5 23 8749.5 24 10735.0 25 13536.5 26 17445.0 27 22108.5 28 26759.0 29 32787.0 30 40148.5 31 46616.5 32 54071.5 33 62107.5 34 70520.5 35 77019.0 36 82923.0 37 91321.5 38 96765.0 39 98457.5 40 99359.5 41 103548.0 42 108453.5 43 113715.0 44 118977.0 45 121334.0 46 121304.5 47 122591.0 48 132141.5 49 135583.0 50 130233.5 51 125048.5 52 119732.5 53 116927.0 54 111572.0 55 101191.5 56 89822.0 57 79923.5 58 71940.0 59 64155.5 60 54902.5 61 44965.5 62 35795.5 63 27653.5 64 21537.5 65 17456.5 66 13892.0 67 11058.5 68 8367.0 69 5780.5 70 4136.0 71 3271.0 72 2723.0 73 2252.0 74 1775.5 75 1452.0 76 1035.0 77 653.5 78 470.0 79 352.5 80 248.0 81 177.0 82 139.0 83 102.5 84 75.5 85 56.0 86 42.0 87 36.0 88 30.0 89 17.5 90 10.5 91 10.0 92 8.5 93 7.0 94 3.5 95 1.5 96 1.0 97 1.5 98 1.0 99 0.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20094121651252445 2 0.23699996013307223 3 0.28867820903479785 4 0.27460577856242235 5 0.30822490421524334 6 0.22030940306118504 7 0.21322856066705106 8 0.19391987699565214 9 0.19314633959125094 10-11 0.13639547040297134 12-13 0.16108916446654778 14-15 0.17748220715212684 16-17 0.1521934843159341 18-19 0.17670866974772564 20-21 0.17229058111104964 22-23 0.19333972394235124 24-25 0.18478618533599192 26-27 0.23732722595801117 28-29 0.16458495850566854 30-31 0.14569279497510101 32-33 0.18426553515995264 34-35 0.14389283293793673 36-37 0.1442498502015065 38-39 0.1762177710103172 40-41 0.1950801831022539 42-43 0.26576960128907035 44-45 0.16260648783671935 46-47 0.14814728866214324 48-49 0.15063153378781632 50-51 0.15586778698683973 52-53 0.17670866974772564 54-55 0.1629337536616583 56-57 0.16107428874723237 58-59 0.13795742093108912 60-61 0.14970923919026105 62-63 0.1392962356694758 64-65 0.1339112252772983 66-67 0.12272468435211184 68-69 0.12525355663573112 70-71 0.1275890445682501 72-73 0.10429366812032197 74-75 0.10554322854281618 76-77 0.12703864295358003 78-79 0.13292942780248138 80-81 0.14301516549832768 82-83 0.14413084444698324 84-85 0.13309306071495086 86-87 0.15668595154918716 88-89 0.152773637369235 90-91 0.14158709644404854 92-93 0.14127470633842498 94-95 0.16125279737901727 96-97 0.15055715519123927 98-99 0.14401183869245998 100-101 0.12596759116287068 102-103 0.16361803675016703 104-105 0.12281393866800429 106-107 0.12830307909538965 108-109 0.13951937145920693 110-111 0.11539095472961594 112-113 0.10823573373890494 114-115 0.10545397422692374 116-117 0.10948529416139917 118-119 0.09453519624941464 120-121 0.08739485097801904 122-123 0.11375462560492113 124-125 0.10987206286359977 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3361182.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.08618080846447 #Duplication Level Percentage of deduplicated Percentage of total 1 74.96446155037683 36.79719113870979 2 11.653195719932915 11.440217442101028 3 4.860122200658943 7.156945112783314 4 2.5879338015255504 5.081271460080798 5 1.5403200700681272 3.7804214731135377 6 0.9413643744931499 2.772478913581069 7 0.6668966680664251 2.291478730048974 8 0.44116583185694525 1.732411663123732 9 0.3555840738707078 1.5708837728365153 >10 1.8054904950224115 15.829650096217888 >50 0.11525159726782297 3.8855636206322686 >100 0.06407783135925989 5.6980996096722265 >500 0.002926863585897524 0.9492118974623307 >1k 0.0012089219150208815 1.0141750696364555 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4495 0.1337327166455134 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.9751438630814992E-5 0.0 0.0 0.0 0.0 3 2.9751438630814992E-5 0.0 0.0 0.0 0.0 4 2.9751438630814992E-5 0.0 0.0 0.0 0.0 5 2.9751438630814992E-5 0.0 0.0 0.0 0.0 6 2.9751438630814992E-5 0.0 0.0 0.0 0.0 7 2.9751438630814992E-5 0.0 0.0 0.0 0.0 8 2.9751438630814992E-5 0.0 0.0 0.0 0.0 9 2.9751438630814992E-5 0.0 0.0 5.9502877261629985E-5 0.0 10-11 2.9751438630814992E-5 0.0 0.0 5.9502877261629985E-5 2.9751438630814992E-5 12-13 7.437859657703748E-5 0.0 0.0 7.437859657703748E-5 2.9751438630814992E-5 14-15 1.1900575452325997E-4 0.0 0.0 8.925431589244498E-5 2.9751438630814992E-5 16-17 1.1900575452325997E-4 0.0 0.0 1.0413003520785247E-4 2.9751438630814992E-5 18-19 1.7850863178488994E-4 0.0 0.0 1.9338435110029744E-4 2.9751438630814992E-5 20-21 2.6776294767733494E-4 0.0 0.0 3.2726582493896494E-4 2.9751438630814992E-5 22-23 2.6776294767733494E-4 0.0 0.0 4.016444215160024E-4 2.9751438630814992E-5 24-25 4.462715794622249E-4 0.0 0.0 5.652773339854849E-4 2.9751438630814992E-5 26-27 4.611472987776324E-4 0.0 0.0 0.0011156789486555622 2.9751438630814992E-5 28-29 5.504016146700774E-4 0.0 0.0 0.0017999620371643072 5.9502877261629985E-5 30-31 5.950287726162999E-4 0.0 0.0 0.003986692776529209 5.9502877261629985E-5 32-33 5.950287726162999E-4 0.0 0.0 0.008389905693889827 5.9502877261629985E-5 34-35 6.099044919317074E-4 0.0 0.0 0.014221187665529565 5.9502877261629985E-5 36-37 6.545316498779299E-4 0.0 0.0 0.02478294837946889 5.9502877261629985E-5 38-39 6.842830885087449E-4 0.0 0.0 0.04260406011932707 5.9502877261629985E-5 40-41 6.991588078241524E-4 0.0 0.0 0.06481350905723046 5.9502877261629985E-5 42-43 7.289102464549674E-4 0.0 0.0 0.08497010872960761 5.9502877261629985E-5 44-45 7.735374044011899E-4 2.9751438630814992E-5 0.0 0.10789359219465057 5.9502877261629985E-5 46-47 8.627917202936348E-4 2.9751438630814992E-5 0.0 0.13627646464844806 5.9502877261629985E-5 48-49 9.222945975552648E-4 2.9751438630814992E-5 0.0 0.16953857303769923 5.9502877261629985E-5 50-51 9.222945975552648E-4 2.9751438630814992E-5 0.0 0.2081261889418663 5.9502877261629985E-5 52-53 9.222945975552648E-4 2.9751438630814992E-5 0.0 0.2434560223159591 5.9502877261629985E-5 54-55 9.222945975552648E-4 2.9751438630814992E-5 0.0 0.28135935513161736 5.9502877261629985E-5 56-57 9.817974748168948E-4 2.9751438630814992E-5 0.0 0.32867901827392865 5.9502877261629985E-5 58-59 9.817974748168948E-4 2.9751438630814992E-5 0.0 0.3832282810035279 5.9502877261629985E-5 60-61 9.817974748168948E-4 2.9751438630814992E-5 0.0 0.445289781987408 5.9502877261629985E-5 62-63 9.817974748168948E-4 2.9751438630814992E-5 0.0 0.5070983957429261 5.9502877261629985E-5 64-65 9.817974748168948E-4 2.9751438630814992E-5 0.0 0.5696210440255839 5.9502877261629985E-5 66-67 0.0010115489134477098 2.9751438630814992E-5 0.0 0.6373948212265803 5.9502877261629985E-5 68-69 0.0010115489134477098 2.9751438630814992E-5 0.0 0.7248938022398073 5.9502877261629985E-5 70-71 0.0010115489134477098 2.9751438630814992E-5 0.0 0.8274767626388575 5.9502877261629985E-5 72-73 0.0010264246327631172 2.9751438630814992E-5 0.0 0.9356083663425545 5.9502877261629985E-5 74-75 0.0010413003520785247 2.9751438630814992E-5 0.0 1.0436209642917282 5.9502877261629985E-5 76-77 0.0010413003520785247 2.9751438630814992E-5 0.0 1.158446641687359 5.9502877261629985E-5 78-79 0.0010413003520785247 2.9751438630814992E-5 0.0 1.2965974469695483 5.9502877261629985E-5 80-81 0.0011008032293401547 2.9751438630814992E-5 0.0 1.4589510475779055 5.9502877261629985E-5 82-83 0.0011008032293401547 2.9751438630814992E-5 0.0 1.6323424319182953 5.9502877261629985E-5 84-85 0.0011751818259171922 2.9751438630814992E-5 0.0 1.803204943975066 5.9502877261629985E-5 86-87 0.0012495604224942297 2.9751438630814992E-5 0.0 1.9828738818665577 5.9502877261629985E-5 88-89 0.0012495604224942297 2.9751438630814992E-5 0.0 2.19321655298642 5.9502877261629985E-5 90-91 0.0013983176156483047 2.9751438630814992E-5 0.0 2.430038004487707 5.9502877261629985E-5 92-93 0.0013983176156483047 2.9751438630814992E-5 0.0 2.6889945263303208 5.9502877261629985E-5 94-95 0.0013983176156483047 2.9751438630814992E-5 0.0 2.9505245476145 5.9502877261629985E-5 96-97 0.0013983176156483047 2.9751438630814992E-5 0.0 3.2149404584458683 5.9502877261629985E-5 98-99 0.0013983176156483047 2.9751438630814992E-5 0.0 3.5080962589946036 5.9502877261629985E-5 100-101 0.0014726962122253422 2.9751438630814992E-5 0.0 3.8581368102054574 5.9502877261629985E-5 102-103 0.0015470748088023797 2.9751438630814992E-5 0.0 4.2362627194838005 5.9502877261629985E-5 104-105 0.001859464914425937 2.9751438630814992E-5 0.0 4.6093903870721675 5.9502877261629985E-5 106-107 0.001948719230318382 2.9751438630814992E-5 0.0 4.982443676063957 5.9502877261629985E-5 108-109 0.0019933463882646045 2.9751438630814992E-5 0.0 5.377260142414187 5.9502877261629985E-5 110-111 0.0019933463882646045 2.9751438630814992E-5 0.0 5.836875242102332 5.9502877261629985E-5 112-113 0.0020230978268954195 2.9751438630814992E-5 0.0 6.337993598680464 5.9502877261629985E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 19925 0.0 35.48798 1 TACATGG 20225 0.0 33.781918 2 GTATCAA 15375 0.0 32.2357 1 ACATGGG 20670 0.0 31.642536 3 TCAACGC 17635 0.0 27.056181 4 ATCAACG 17695 0.0 27.031765 3 CAACGCA 17825 0.0 26.869642 5 CGCATAG 405 0.0 26.444063 8 AACGCAG 17845 0.0 26.18299 6 TATCAAC 18875 0.0 26.130806 2 AACGCAT 480 0.0 26.04019 6 CATGGGA 14395 0.0 26.028458 4 CATGGGG 7160 0.0 24.70887 4 GGACCGA 540 0.0 24.249067 6 ATGGGAT 5560 0.0 23.464142 5 GGTATCA 11310 0.0 23.22763 1 ATGGGAG 5600 0.0 22.871033 5 ACGCAGA 20615 0.0 22.17069 7 GTTATCA 2105 0.0 22.07351 1 ACGCATA 490 0.0 21.86139 7 >>END_MODULE