FastQCFastQC Report
Thu 26 May 2016
SRR1364623_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364623_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3361182
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138810.41297971963434293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116160.34559271113554696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80100.2383090234328281No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA213000.038.7452161
GGTATCA144900.038.4495351
ATCAACG265200.030.689823
TATCAAC266050.030.5953542
TCAACGC266750.030.4254444
GTACATG208500.029.9218561
CAACGCA268650.029.684835
AACGCAG272700.029.3461726
TACATGG212650.028.7017752
ACATGGG215300.027.2191143
GAGTACT163550.025.05409212-13
ACGCAGA319350.024.9109237
CGCAGAG320350.024.851758
AGTACTT171700.024.48923512-13
CATGGGA151600.023.4316124
GTACTTT176900.023.23068814-15
GCAGAGT338000.022.814449
ATGGGAG56000.022.2279325
CATGGGG74350.020.8074764
TACTTTT203050.020.79620414-15
Produced by FastQC (version 0.11.5)