FastQCFastQC Report
Thu 26 May 2016
SRR1364622_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364622_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301285
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51440.1558181132498406No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105900.040.559161
GTACATG176350.035.7404371
ATCAACG124150.033.6842463
TCAACGC124350.033.5821654
TACATGG182200.033.251332
CAACGCA125850.033.2307475
ACATGGG184200.032.2758263
AACGCAG126950.031.927756
TATCAAC137600.031.2575682
CGCATAG4600.031.0438378
GGTATCA75000.029.707081
CATGGGA125950.029.0406724
ACGCATA5450.027.2976237
TACCTGG31300.027.0259192
ACGCAGA148950.026.728037
CGCAGAG146650.025.5611558
GTACCTG34950.024.3746571
AACGCAT6400.024.1794956
ATGGGAG54950.023.6344265
CGCAGAT9800.023.0716978
Produced by FastQC (version 0.11.5)