##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364622_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3301285 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.22246307119803 31.0 27.0 31.0 10.0 34.0 2 26.720088389823964 31.0 26.0 31.0 10.0 34.0 3 27.40826950717675 31.0 27.0 33.0 12.0 34.0 4 30.810918172772116 35.0 31.0 37.0 12.0 37.0 5 31.10257551226265 35.0 32.0 37.0 12.0 37.0 6 31.14755648179421 35.0 32.0 37.0 11.0 37.0 7 30.878095953545362 35.0 31.0 37.0 10.0 37.0 8 30.68883510511816 35.0 31.0 37.0 10.0 37.0 9 31.62339694997554 37.0 30.0 39.0 8.0 39.0 10-11 31.6297788588383 37.0 30.5 39.0 8.0 39.0 12-13 31.65169002373318 37.0 31.0 39.0 8.0 39.0 14-15 32.34919872110406 38.0 30.5 40.0 8.0 41.0 16-17 32.760547938151355 38.0 31.5 40.0 8.0 41.0 18-19 32.607122529560456 38.0 31.0 40.0 8.0 41.0 20-21 32.53550996051538 38.0 31.0 40.0 8.0 41.0 22-23 32.61426005328228 38.0 31.0 40.0 8.0 41.0 24-25 32.72578177891336 38.0 31.0 40.0 8.0 41.0 26-27 32.54505412286428 38.0 30.5 40.0 5.0 41.0 28-29 32.45574617156653 38.0 30.0 40.0 2.0 41.0 30-31 32.34045909395887 38.0 30.0 40.0 2.0 41.0 32-33 32.24922901233913 38.0 30.0 40.0 2.0 41.0 34-35 32.316689713248024 38.0 30.0 40.0 2.0 41.0 36-37 32.25739325747398 38.0 30.0 40.0 2.0 41.0 38-39 31.967272592339043 38.0 30.0 40.0 2.0 41.0 40-41 31.83915202716518 38.0 30.0 40.0 2.0 41.0 42-43 31.339897797372842 37.0 29.5 40.0 2.0 41.0 44-45 31.232368305069087 37.0 29.0 40.0 2.0 41.0 46-47 31.219275373074424 37.0 29.5 40.0 2.0 41.0 48-49 31.164458233687792 37.0 29.0 40.0 2.0 41.0 50-51 30.934995463887546 37.0 29.0 40.0 2.0 41.0 52-53 30.844692142605076 37.0 29.0 40.0 2.0 41.0 54-55 30.684259462603197 36.0 28.5 40.0 2.0 41.0 56-57 30.46923834203954 36.0 28.0 40.0 2.0 41.0 58-59 30.270196605261283 36.0 28.0 40.0 2.0 41.0 60-61 29.978284516483733 35.0 28.0 39.0 2.0 41.0 62-63 29.808634062190933 35.0 28.0 39.0 2.0 40.5 64-65 29.58292740554057 35.0 28.0 39.0 2.0 40.0 66-67 29.322068376404943 35.0 28.0 39.0 2.0 40.0 68-69 29.0775114841645 34.0 27.5 38.0 2.0 40.0 70-71 28.63939057064143 34.0 26.0 37.5 2.0 40.0 72-73 28.40256975692798 34.0 26.0 37.0 2.0 39.5 74-75 28.351460870539803 34.0 26.0 37.0 2.0 39.0 76-77 28.080797022977414 34.0 26.0 36.0 2.0 39.0 78-79 27.77696306135338 34.0 26.0 36.0 2.0 38.5 80-81 27.355640606612276 33.5 26.0 35.5 2.0 37.0 82-83 27.45002703492731 34.0 26.0 35.0 2.0 37.0 84-85 27.2658014985074 34.0 26.0 35.0 2.0 37.0 86-87 27.128971445967252 34.0 26.0 35.0 2.0 36.0 88-89 26.952061545731436 34.0 26.0 35.0 2.0 36.0 90-91 26.814253086298216 34.0 26.0 35.0 2.0 36.0 92-93 26.683370869222138 33.0 25.5 35.0 2.0 35.0 94-95 26.573476085827185 33.0 25.0 35.0 2.0 35.0 96-97 26.45846056914202 33.0 25.0 35.0 2.0 35.0 98-99 26.368488028146615 33.0 25.0 35.0 2.0 35.0 100-101 26.26953973982858 33.0 25.0 35.0 2.0 35.0 102-103 25.11288240791086 31.5 21.5 34.0 2.0 35.0 104-105 25.964051422400672 33.0 24.0 35.0 2.0 35.0 106-107 26.123354087877903 33.0 24.0 35.0 2.0 35.0 108-109 26.082268571177586 33.0 24.0 35.0 2.0 35.0 110-111 26.00976574273351 33.0 24.0 35.0 2.0 35.0 112-113 25.92339134609705 33.0 23.5 35.0 2.0 35.0 114-115 25.817358695174757 33.0 23.0 35.0 2.0 35.0 116-117 25.713587739319692 33.0 23.0 35.0 2.0 35.0 118-119 25.590056750628925 33.0 21.0 35.0 2.0 35.0 120-121 25.406869446291367 33.0 19.0 35.0 2.0 35.0 122-123 25.20870494368102 33.0 16.5 35.0 2.0 35.0 124-125 24.26721579627327 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.519859681447798 1101 2 1.8287120154908152 1101 3 1.3330712633856088 1101 4 1.4065782615255955 1101 5 1.1027995056590747 1101 6 1.1343727874741845 1101 7 1.213066826394531 1101 8 1.3820067152217206 1101 9 1.7261681194599028 1101 10-11 1.7382591175646738 1101 12-13 1.7203513378897561 1101 14-15 1.959015622433327 1101 16-17 1.5623102383818548 1101 18-19 1.5332494631609421 1101 20-21 1.8122568606930827 1101 22-23 1.5579947257160356 1101 24-25 1.5846641877943313 1101 26-27 1.8102841599428814 1101 28-29 1.8451171755062248 1101 30-31 1.984013120773085 1101 32-33 1.9843292000994524 1101 34-35 1.7125426906775303 1101 36-37 1.6723042587472037 1101 38-39 1.624225615325777 1101 40-41 1.6774047195539694 1101 42-43 1.8702616033328248 1101 44-45 1.7219115022166527 1101 46-47 1.4066375890098506 1101 48-49 1.3417200792809787 1101 50-51 1.3054540735055546 1101 52-53 1.276203270149992 1101 54-55 1.3248422602731402 1101 56-57 1.2882572690083904 1101 58-59 1.0572038988343273 1101 60-61 1.1156678972609235 1101 62-63 1.050098523179777 1101 64-65 0.9957679423880172 1101 66-67 1.0232013200056684 1101 68-69 0.9466021495374903 1101 70-71 0.8537173717783979 1101 72-73 0.7853104113670994 1101 74-75 0.8166302489013368 1101 76-77 0.957766589807477 1101 78-79 0.8487544876095043 1101 80-81 0.9881635013671044 1101 82-83 0.9391027492222257 1101 84-85 0.9512232357873494 1101 86-87 0.8796898657282917 1101 88-89 0.8313044660712166 1101 90-91 0.888119793048137 1101 92-93 0.9308830208701124 1101 94-95 0.8816964038144874 1101 96-97 0.8792358420631139 1101 98-99 0.9441536464751223 1101 100-101 0.9371050222526378 1101 102-103 0.782248492090492 1101 104-105 0.9955486096789237 1101 106-107 1.0191786036116568 1101 108-109 1.029407549355863 1101 110-111 1.0975204264814025 1101 112-113 0.9450679032693436 1101 114-115 0.8895519638919929 1101 116-117 0.9533304928579831 1101 118-119 0.869581446026924 1101 120-121 0.9458813998892097 1101 122-123 0.9304230445283039 1101 124-125 0.7843210895718151 1102 1 1.1261254948886474 1102 2 1.6237661311853842 1102 3 1.2602469637587674 1102 4 1.1406651673602113 1102 5 1.0378335634198201 1102 6 0.825817493248735 1102 7 0.8796376578824372 1102 8 0.8494213544589115 1102 9 1.547234168097848 1102 10-11 1.3981264501140132 1102 12-13 1.5897981792742648 1102 14-15 1.8040395748644542 1102 16-17 1.479406763370271 1102 18-19 1.374553470675032 1102 20-21 1.588597153213101 1102 22-23 1.6446429635196438 1102 24-25 1.3059866878070565 1102 26-27 1.732246706587759 1102 28-29 1.3908290921588957 1102 30-31 1.492207410370952 1102 32-33 1.7331418238706462 1102 34-35 1.518502416993634 1102 36-37 1.421923336184058 1102 38-39 1.6573526421189015 1102 40-41 1.7377157829808212 1102 42-43 1.9106274315371259 1102 44-45 1.465734842218989 1102 46-47 1.2543968530771288 1102 48-49 1.2292817421549636 1102 50-51 1.3159535618755207 1102 52-53 1.24151475357165 1102 54-55 1.2796703551257842 1102 56-57 1.280136160323913 1102 58-59 1.2349096835628046 1102 60-61 1.1714087171926018 1102 62-63 0.943211371966374 1102 64-65 0.918451798296708 1102 66-67 1.0000803513194683 1102 68-69 0.9086332189715414 1102 70-71 1.0890538258004199 1102 72-73 0.8007730292838211 1102 74-75 0.9626114076797379 1102 76-77 0.603249863832449 1102 78-79 0.6651228943273857 1102 80-81 0.8977100469734474 1102 82-83 0.8438913263816339 1102 84-85 0.8753329104313714 1102 86-87 0.9375172962255149 1102 88-89 0.8598258679884303 1102 90-91 0.9986178177998326 1102 92-93 1.0621739703638262 1102 94-95 0.9984798417647092 1102 96-97 0.7734136958641962 1102 98-99 0.9332836081410534 1102 100-101 0.9359998631891493 1102 102-103 0.8738956862706395 1102 104-105 0.9012969386086738 1102 106-107 0.8854635204526886 1102 108-109 0.9628157817631724 1102 110-111 0.9509464386688862 1102 112-113 0.8734556068395527 1102 114-115 0.8997871100756392 1102 116-117 1.0370069492217944 1102 118-119 1.0104050049356523 1102 120-121 0.9988288725386383 1102 122-123 0.9858422502544855 1102 124-125 0.9529227868599257 1103 1 1.2269454176071015 1103 2 1.577424891309441 1103 3 1.2146887085262819 1103 4 1.0606123046807632 1103 5 1.0622437559357643 1103 6 0.967246567337881 1103 7 0.9737135298421222 1103 8 1.1561554283055777 1103 9 1.7804784355927517 1103 10-11 1.676398102843983 1103 12-13 1.706341822282834 1103 14-15 1.8955335843376062 1103 16-17 1.5436013776750315 1103 18-19 1.4798333935601278 1103 20-21 1.8092245151188564 1103 22-23 1.579441663829229 1103 24-25 1.4114967846771052 1103 26-27 1.7128998076449093 1103 28-29 1.6665523595356575 1103 30-31 1.6769420512138709 1103 32-33 1.8107479076079187 1103 34-35 1.6073390641786176 1103 36-37 1.491691839777566 1103 38-39 1.6216166248039343 1103 40-41 1.7528806039187472 1103 42-43 1.912610422931568 1103 44-45 1.7506714567814008 1103 46-47 1.5972384827362909 1103 48-49 1.4524724654640089 1103 50-51 1.4649553258875763 1103 52-53 1.3631959722162108 1103 54-55 1.3927512191733733 1103 56-57 1.402114293581704 1103 58-59 1.3805566628123245 1103 60-61 1.5234902920150653 1103 62-63 1.2195757681057593 1103 64-65 1.0795765359754874 1103 66-67 1.036058207274646 1103 68-69 1.1071976759124063 1103 70-71 1.2551850719722886 1103 72-73 1.223911966650821 1103 74-75 1.1990671513154005 1103 76-77 1.1272902269578715 1103 78-79 1.1057716756165945 1103 80-81 1.2644471929399437 1103 82-83 1.2152660914595366 1103 84-85 1.256567599236103 1103 86-87 1.0875811206899684 1103 88-89 1.1528132951564345 1103 90-91 1.2219281785987306 1103 92-93 1.14525389653895 1103 94-95 1.1132775465194662 1103 96-97 1.1987159995054277 1103 98-99 1.2160257079541807 1103 100-101 1.1923035518479637 1103 102-103 1.0357493367202295 1103 104-105 1.02237080471766 1103 106-107 1.2121391556583276 1103 108-109 1.3383037264701407 1103 110-111 1.1821200803080316 1103 112-113 1.2133915836914078 1103 114-115 1.333556757061725 1103 116-117 1.3737684520993945 1103 118-119 1.3259508071872723 1103 120-121 1.3657507943354616 1103 122-123 1.2532043311948016 1103 124-125 1.0940097831834201 1104 1 1.3841608404168326 1104 2 1.7257297659465536 1104 3 1.2699220877039963 1104 4 1.3949556383435535 1104 5 0.9354761785796519 1104 6 0.995992084109055 1104 7 0.8264774686842635 1104 8 1.2793700939291597 1104 9 1.5866590512922976 1104 10-11 1.6349442133026955 1104 12-13 1.5889627040361525 1104 14-15 1.7501951162658997 1104 16-17 1.3835016745187971 1104 18-19 1.3722429165161003 1104 20-21 1.6566745818752082 1104 22-23 1.4996073359910795 1104 24-25 1.4540379985537157 1104 26-27 1.6748614223276164 1104 28-29 1.6232808881990053 1104 30-31 1.7147510819988412 1104 32-33 1.7024538923938408 1104 34-35 1.6127710581412344 1104 36-37 1.4887390650756274 1104 38-39 1.5615745900839357 1104 40-41 1.5584334210030093 1104 42-43 1.7666461455334037 1104 44-45 1.5665120171661577 1104 46-47 1.4011093665836007 1104 48-49 1.313857751762452 1104 50-51 1.2248995713566053 1104 52-53 1.2102060711027107 1104 54-55 1.3358898897756184 1104 56-57 1.3418966779474886 1104 58-59 1.3127013506991894 1104 60-61 1.294109542970343 1104 62-63 1.2163463775568495 1104 64-65 1.1863335219390656 1104 66-67 1.1274524809706357 1104 68-69 1.033396588881228 1104 70-71 1.1266273021870283 1104 72-73 1.0577805722389222 1104 74-75 1.0016842387620208 1104 76-77 1.1409407478583837 1104 78-79 1.159006573354585 1104 80-81 1.243673594111236 1104 82-83 1.2026511105650854 1104 84-85 1.2485398765069498 1104 86-87 1.0989765209516378 1104 88-89 1.1172248551441726 1104 90-91 1.1140633681549232 1104 92-93 1.098275207476103 1104 94-95 1.103003770120555 1104 96-97 1.0433177815731831 1104 98-99 1.0860348754347129 1104 100-101 1.1237582496687075 1104 102-103 0.9738474635221053 1104 104-105 1.066944129485183 1104 106-107 1.0150962649159325 1104 108-109 1.041364823183919 1104 110-111 1.0148229626409702 1104 112-113 1.0630089701595544 1104 114-115 1.0901851721471516 1104 116-117 1.032129077079503 1104 118-119 1.0273740491166201 1104 120-121 1.0453727708258356 1104 122-123 0.9941924549310812 1104 124-125 0.7423906277140055 1105 1 1.2509526968507956 1105 2 1.5729287483929681 1105 3 1.119882609134848 1105 4 1.1688548791000741 1105 5 1.0114776531434266 1105 6 0.9352640443899531 1105 7 0.8288883412390753 1105 8 0.821731448331672 1105 9 1.1047685376525855 1105 10-11 1.116655472015438 1105 12-13 1.2103924753218926 1105 14-15 1.4786023324777346 1105 16-17 1.0240803492938255 1105 18-19 1.1777183130011935 1105 20-21 1.1380529852677483 1105 22-23 1.3085874359163014 1105 24-25 1.0633736997297092 1105 26-27 1.4454883210899254 1105 28-29 1.482956307691481 1105 30-31 1.3887496074350736 1105 32-33 1.460910247659129 1105 34-35 1.2585201835994226 1105 36-37 0.9801583588537 1105 38-39 1.2090958392277003 1105 40-41 1.2983536403051588 1105 42-43 1.4909033732489299 1105 44-45 1.2449755753094571 1105 46-47 0.8863071908176927 1105 48-49 0.9783315821771588 1105 50-51 1.1106035579077478 1105 52-53 0.8903724693499697 1105 54-55 0.9395832275709921 1105 56-57 1.0452668666602776 1105 58-59 1.0355591807897042 1105 60-61 0.8877600295240988 1105 62-63 0.9039416424815769 1105 64-65 0.6062247074988001 1105 66-67 0.5653400768564971 1105 68-69 0.44559059728938166 1105 70-71 0.687891636045002 1105 72-73 0.7222026033720859 1105 74-75 0.7129220357009949 1105 76-77 0.7597130689368043 1105 78-79 0.852217239888784 1105 80-81 0.9369321382148144 1105 82-83 0.8248621195954833 1105 84-85 0.7716794489357461 1105 86-87 0.7972914592761775 1105 88-89 0.8074243003526256 1105 90-91 0.6839946859024977 1105 92-93 0.7732680503513976 1105 94-95 0.6581164082301854 1105 96-97 0.6571732560219168 1105 98-99 0.7476345810448493 1105 100-101 0.7210521702305037 1105 102-103 0.6247807656560376 1105 104-105 0.7086429432979777 1105 106-107 0.6937742099910444 1105 108-109 0.6918517037494816 1105 110-111 0.6823835073406457 1105 112-113 0.6698854309973363 1105 114-115 0.787108556271015 1105 116-117 0.7411279031803275 1105 118-119 0.7166914358967915 1105 120-121 0.5824403040165933 1105 122-123 0.626296963331388 1105 124-125 0.5629012150296226 1106 1 0.4549808925451231 1106 2 -2.248184306262825 1106 3 -0.13482199490577074 1106 4 0.41052958883819457 1106 5 0.5192325913732709 1106 6 0.7314367147233938 1106 7 0.7327413254846675 1106 8 0.7843572869661095 1106 9 1.1883960239970115 1106 10-11 1.3672569438579956 1106 12-13 1.3419144960554767 1106 14-15 1.5408515759600832 1106 16-17 1.120630559240908 1106 18-19 1.1489356891652989 1106 20-21 1.4068520379925644 1106 22-23 1.0921289999699226 1106 24-25 1.1966187700355846 1106 26-27 1.1882271110031084 1106 28-29 1.325544382880416 1106 30-31 1.4448799848592984 1106 32-33 1.5773015991733317 1106 34-35 1.3552885681165066 1106 36-37 1.1651022665313562 1106 38-39 1.4016114635460255 1106 40-41 1.3433934705012476 1106 42-43 1.6022977920897254 1106 44-45 1.5088161060984326 1106 46-47 1.2738601517955424 1106 48-49 1.0382178935070918 1106 50-51 1.0618321166546743 1106 52-53 1.123215831310631 1106 54-55 1.0403787724312572 1106 56-57 1.0505909426370295 1106 58-59 0.9516235786832112 1106 60-61 1.098211512178569 1106 62-63 1.03780758758489 1106 64-65 0.9853505818568031 1106 66-67 0.77664236525127 1106 68-69 0.8290611330922957 1106 70-71 0.8439716922416487 1106 72-73 0.7220686399972784 1106 74-75 0.7796668173730978 1106 76-77 0.7116024741489042 1106 78-79 0.8352159835573367 1106 80-81 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2216 86-87 -2.2743287024434906 2216 88-89 -2.3547815808830883 2216 90-91 -2.1241811188534285 2216 92-93 -2.0921844489896984 2216 94-95 -2.357059840227741 2216 96-97 -2.324473851426724 2216 98-99 -2.31317984077506 2216 100-101 -2.370178758034406 2216 102-103 -2.13350493460419 2216 104-105 -2.146709893297544 2216 106-107 -2.143770134641958 2216 108-109 -2.2195612263434192 2216 110-111 -2.0959432751667713 2216 112-113 -2.3381214397362164 2216 114-115 -2.3046286408004626 2216 116-117 -2.106760801396831 2216 118-119 -2.195263666778512 2216 120-121 -2.415209350079934 2216 122-123 -2.2421697182568323 2216 124-125 -1.9998442126628753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 247288.0 3 34206.0 4 20723.0 5 16967.0 6 16821.0 7 17870.0 8 19859.0 9 21088.0 10 22855.0 11 24153.0 12 25746.0 13 26831.0 14 27807.0 15 24324.0 16 19661.0 17 17522.0 18 15586.0 19 14718.0 20 14201.0 21 15029.0 22 19004.0 23 26653.0 24 40133.0 25 53083.0 26 60757.0 27 63149.0 28 72021.0 29 82468.0 30 96220.0 31 111454.0 32 135102.0 33 171920.0 34 227157.0 35 313513.0 36 462806.0 37 561253.0 38 161112.0 39 225.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.99277992364712 19.34575570112259 14.022330395890128 22.639133979340166 2 13.206515402515508 21.01914444503821 41.97376762872675 23.800572523719534 3 18.639193371948164 26.95946658193155 28.547399362117147 25.853940684003142 4 11.498596717168649 17.265026060966868 37.80295114631805 33.43342607554643 5 12.732010463126109 40.517115913243195 33.330203753854626 13.420669869776072 6 30.845876719469263 37.92569870918561 17.826863474952845 13.401561096392284 7 28.5773002350747 33.77292464010258 20.581451683406833 17.068323441415888 8 25.05518345064021 37.609907861164416 19.744005846577718 17.590902841617652 9 26.01408092495373 17.980827653436133 20.11351296558313 35.891578456027 10-11 23.516097559791902 29.066286781818125 27.465562319208527 19.95205333918145 12-13 24.420717679766955 26.220916800124026 27.755384448982273 21.602981071126745 14-15 21.710670154921672 27.429725230883466 25.632499094582073 25.227105519612785 16-17 21.651492095389262 30.741066357957276 25.937049495434888 21.670392051218577 18-19 21.64913213393913 29.335317252602565 27.954114356650912 21.061436256807394 20-21 22.74855437220292 28.288547217267002 27.819668305909616 21.143230104620457 22-23 22.435588198472644 28.105996744981848 27.555511716927235 21.902903339618277 24-25 22.008113821096266 28.1609860764378 27.915601936914186 21.91529816555175 26-27 21.887180754637292 28.28829422410486 27.784998251854148 22.039526769403697 28-29 21.78764135761565 28.326439911938966 27.987836994856636 21.898081735588747 30-31 21.87189444856327 28.140709767524257 28.02347994064036 21.963915843272108 32-33 21.698841101279253 28.19000616191747 27.8588163747077 22.252336362095573 34-35 22.213539833961455 28.015181776044535 27.846901519876333 21.924376870117673 36-37 22.215895802347948 27.825571402613797 27.653180674159078 22.305352120879178 38-39 21.93461162252018 28.124294023606865 27.858145190545542 22.082949163327413 40-41 22.078850914461253 27.894008355733096 28.0535445550324 21.97359617477325 42-43 21.795011190214204 27.850862340472254 27.991055117689346 22.363071351624196 44-45 22.021735021333622 28.04566545033315 27.772255884532964 22.160343643800264 46-47 22.14091100549939 28.259086688994685 27.282462243927686 22.317540061578242 48-49 21.989946440678995 28.301027349989155 27.308822347175433 22.400203862156417 50-51 22.197024707839876 28.264592912918452 27.348911869359092 22.189470509882575 52-53 22.30144594805159 28.20623576950463 27.27354518764037 22.218773094803414 54-55 22.230751201663253 28.201726208129518 27.306871078997652 22.26065151120958 56-57 22.16785691693501 28.480827835912788 27.13811570364278 22.21319954350943 58-59 22.225002703386004 28.008231639762478 27.241425488994373 22.52534016785714 60-61 22.135741755266462 28.238138416813367 27.459237823829007 22.16688200409116 62-63 22.21187531764281 28.221377719329567 27.49842230769709 22.06832465533054 64-65 22.09291480234118 28.08567068172474 27.397080361268785 22.424334154665296 66-67 22.06815136964646 28.370610129371293 27.200164983846598 22.361073517135647 68-69 22.14616366110664 28.144373639551773 27.43252009983116 22.276942599510427 70-71 22.028089422473542 28.060063455513962 27.579597666200705 22.332249455811798 72-73 22.271009567531795 28.193312506775204 27.10956836566605 22.42610956002696 74-75 22.060495978786 28.42622669958738 27.172975501258783 22.340301820367834 76-77 22.20832631966844 28.1363062173675 27.403002910102252 22.252364552861803 78-79 22.17920082209614 28.176204708732772 27.337305099458952 22.307289369712137 80-81 22.03918675568165 28.355045359633575 27.36995421189447 22.235813672790304 82-83 22.245141719594276 28.386690681581193 27.174443075173006 22.193724523651532 84-85 22.151775106560383 28.18960028899376 27.278062771216007 22.380561833229855 86-87 22.112981450390063 28.278477149290655 27.331716489681547 22.276824910637735 88-89 22.260323789671865 28.13529324412035 27.335746842716237 22.26863612349155 90-91 22.126307340764463 28.207347225521 27.4328087741793 22.233536659535236 92-93 22.189257841885837 28.266901369144975 27.289213675918873 22.254627113050315 94-95 22.028722299276655 28.097422626535927 27.41138112272397 22.462473951463444 96-97 22.277726096294206 27.966929648144408 27.311571695683718 22.443772559877672 98-99 21.910726297740286 28.120768773997646 27.255530217598867 22.7129747106632 100-101 21.835226558439913 28.163128081921624 27.474442712141073 22.527202647497393 102-103 21.997844003030412 27.968842111906135 27.50639198791962 22.526921897143833 104-105 21.865058063185604 28.01890654996406 27.564697461839643 22.55133792501069 106-107 21.868327863562104 28.112268488855452 27.417892392329467 22.601511255252973 108-109 21.932616688807453 28.15987713798605 27.124298144223637 22.783208028982866 110-111 21.836850429041704 28.195159008511084 27.39377066928686 22.574219893160354 112-113 21.81950667699483 28.196087153821885 27.644064026738 22.340342142445284 114-115 21.73294321738917 28.428072094833475 27.25827007695683 22.580714610820532 116-117 21.63669929750996 28.381343591802523 27.386604231194 22.595352879493515 118-119 21.5313681330476 28.359299106054596 27.406730604804817 22.702602156092983 120-121 21.569973910168578 28.41373794628111 27.457914211128596 22.558373932421713 122-123 21.57806394679743 28.33268892234772 27.43532009030851 22.65392704054634 124-125 21.635232293630953 28.2913451095547 27.398022949697676 22.67539964711667 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6963.0 1 5491.0 2 3411.5 3 2258.0 4 1463.5 5 1126.0 6 1069.5 7 1172.0 8 1361.0 9 1438.0 10 1405.5 11 1443.0 12 1489.5 13 1613.0 14 1743.5 15 1887.5 16 2124.5 17 2369.0 18 2657.0 19 3062.5 20 3667.0 21 4740.0 22 6180.0 23 7758.5 24 9809.5 25 12805.5 26 17110.5 27 22404.0 28 27294.5 29 33136.5 30 41214.0 31 49547.5 32 59488.5 33 69492.5 34 78482.0 35 86849.0 36 95361.0 37 103441.0 38 106510.5 39 108486.0 40 110304.0 41 112471.5 42 115080.0 43 118650.0 44 123648.5 45 125792.5 46 124250.0 47 122147.0 48 121837.0 49 118297.5 50 113387.0 51 108225.5 52 102436.0 53 99554.5 54 95962.0 55 89193.0 56 81270.5 57 73955.0 58 68214.5 59 60875.0 60 50307.0 61 40898.0 62 33200.0 63 25403.0 64 19388.0 65 15354.0 66 11507.0 67 8471.5 68 6352.0 69 4582.5 70 3431.0 71 2553.5 72 1902.5 73 1606.5 74 1321.0 75 1001.0 76 709.0 77 479.5 78 348.5 79 259.0 80 197.0 81 146.0 82 111.0 83 92.0 84 70.5 85 47.5 86 31.5 87 23.5 88 16.0 89 14.0 90 12.0 91 12.5 92 12.0 93 6.0 94 5.5 95 6.0 96 2.5 97 1.5 98 2.0 99 2.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19940720052949076 2 0.23618075991621446 3 0.28649450138355215 4 0.27277257189246007 5 0.30618380418533997 6 0.22167125831305085 7 0.21243243161375042 8 0.19283400251720165 9 0.19177380928941307 10-11 0.13620453853575198 12-13 0.16019519671885343 14-15 0.17706741465823156 16-17 0.15088064193185383 18-19 0.17532566864115035 20-21 0.1713878080807928 22-23 0.19303089554521952 24-25 0.18306507920400691 26-27 0.23613532306359492 28-29 0.1632394658443606 30-31 0.144458900094963 32-33 0.18309537043908658 34-35 0.14239909610954524 36-37 0.14248996981478423 38-39 0.17449265967645933 40-41 0.1945151660641235 42-43 0.26280675555124744 44-45 0.1609676232133851 46-47 0.1462612285822036 48-49 0.1496387012935872 50-51 0.15462160946419348 52-53 0.17464411585185768 54-55 0.16152801106235906 56-57 0.15961966325233962 58-59 0.13611366483051296 60-61 0.14866938177103764 62-63 0.13750706164417795 64-65 0.13341774490842204 66-67 0.12093775605559653 68-69 0.12473930605809556 70-71 0.12637503275239792 72-73 0.10336883970938589 74-75 0.10488340146336958 76-77 0.1257086255806451 78-79 0.1316759988913408 80-81 0.14195987320088996 82-83 0.1430503576637582 84-85 0.131100465424827 86-87 0.15603015189539832 88-89 0.15080491384415462 90-91 0.1397031761874543 92-93 0.14021812718380874 94-95 0.159771119427738 96-97 0.14898743973937423 98-99 0.1435047261899533 100-101 0.1252088202018305 102-103 0.1624216024972094 104-105 0.12161930884488918 106-107 0.12796532259408078 108-109 0.13868841981228522 110-111 0.11571251800435284 112-113 0.10621621580687521 114-115 0.10486825584582973 116-117 0.10865466023078892 118-119 0.0938119550417489 120-121 0.08704186400144186 122-123 0.11207756979479203 124-125 0.10888184449388646 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3301285.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.93355317926226 #Duplication Level Percentage of deduplicated Percentage of total 1 75.34793313888161 39.13085892615605 2 12.16501821585407 12.635452408795029 3 4.674456016723509 7.2828333038589825 4 2.4331860689865032 5.054559924350026 5 1.423750542653652 3.6970212260453468 6 0.8860941792252599 2.7610811507177866 7 0.6020226640878229 2.1885623228368263 8 0.42197509898882996 1.7531732994928682 9 0.3283824587723673 1.534866109721247 >10 1.5610131337322295 14.363236410998931 >50 0.102385679963039 3.66335795866679 >100 0.05092629801602419 4.655444591605611 >500 0.0023095139660636657 0.7500748035211597 >1k 4.862134657783486E-4 0.3671149650055196 >5k 6.0776683222293576E-5 0.16236259822787316 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5144 0.1558181132498406 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.029123507967352E-5 0.0 0.0 0.0 0.0 8 3.029123507967352E-5 0.0 0.0 0.0 0.0 9 3.029123507967352E-5 0.0 0.0 0.0 0.0 10-11 3.029123507967352E-5 0.0 0.0 6.058247015934704E-5 0.0 12-13 3.029123507967352E-5 0.0 0.0 6.058247015934704E-5 0.0 14-15 3.029123507967352E-5 0.0 0.0 9.087370523902057E-5 0.0 16-17 3.029123507967352E-5 0.0 0.0 9.087370523902057E-5 0.0 18-19 3.029123507967352E-5 0.0 0.0 1.3631055785853086E-4 0.0 20-21 3.029123507967352E-5 0.0 0.0 1.6660179293820435E-4 0.0 22-23 3.029123507967352E-5 0.0 0.0 2.574754981772249E-4 0.0 24-25 6.058247015934704E-5 1.514561753983676E-5 0.0 5.603878489739602E-4 0.0 26-27 6.058247015934704E-5 3.029123507967352E-5 0.0 7.724264945316748E-4 0.0 28-29 6.058247015934704E-5 3.029123507967352E-5 0.0 0.0012722318733462878 0.0 30-31 9.087370523902057E-5 3.029123507967352E-5 0.0 0.0031957253009055565 0.0 32-33 9.087370523902057E-5 3.029123507967352E-5 0.0 0.006391450601811113 0.0 34-35 1.0601932277885733E-4 3.029123507967352E-5 0.0 0.011465232477656427 0.0 36-37 1.2116494031869408E-4 3.029123507967352E-5 0.0 0.019840758977186155 0.0 38-39 1.2116494031869408E-4 3.029123507967352E-5 0.0 0.035471036278297695 0.0 40-41 1.3631055785853086E-4 3.029123507967352E-5 0.0 0.054448495055713156 0.0 42-43 1.514561753983676E-4 3.029123507967352E-5 0.0 0.07229003251764085 0.0 44-45 2.1203864555771466E-4 3.029123507967352E-5 0.0 0.0926154512561018 0.0 46-47 2.4232988063738818E-4 3.029123507967352E-5 0.0 0.11913542756835596 0.0 48-49 2.726211157170617E-4 3.029123507967352E-5 0.0 0.15018394352502132 0.0 50-51 2.726211157170617E-4 3.029123507967352E-5 0.0 0.18494313577894667 0.0 52-53 2.726211157170617E-4 3.029123507967352E-5 0.0 0.21709728181602012 0.0 54-55 2.726211157170617E-4 3.029123507967352E-5 0.0 0.251159775663113 0.0 56-57 3.029123507967352E-4 3.029123507967352E-5 0.0 0.2928102238976641 0.0 58-59 3.029123507967352E-4 3.029123507967352E-5 0.0 0.34321483907024086 0.0 60-61 3.029123507967352E-4 3.029123507967352E-5 0.0 0.3999200311393897 0.0 62-63 3.029123507967352E-4 3.029123507967352E-5 0.0 0.45535299133519225 0.0 64-65 3.029123507967352E-4 3.029123507967352E-5 0.0 0.5141482786248385 0.0 66-67 3.332035858764087E-4 3.029123507967352E-5 0.0 0.5798651131301902 0.0 68-69 3.6349482095608226E-4 3.029123507967352E-5 0.0 0.6578498978428097 0.0 70-71 3.6349482095608226E-4 3.029123507967352E-5 0.0 0.7527826285825066 0.0 72-73 3.6349482095608226E-4 3.029123507967352E-5 0.0 0.84709438900307 0.0 74-75 3.6349482095608226E-4 3.029123507967352E-5 0.0 0.9419816828901473 0.0 76-77 3.6349482095608226E-4 3.029123507967352E-5 0.0 1.0479404231988454 0.0 78-79 3.6349482095608226E-4 3.029123507967352E-5 0.0 1.1756634159122887 0.0 80-81 3.6349482095608226E-4 3.029123507967352E-5 0.0 1.323530079953715 0.0 82-83 3.6349482095608226E-4 3.029123507967352E-5 0.0 1.481256541013575 0.0 84-85 3.78640438495919E-4 3.029123507967352E-5 0.0 1.6394373705996301 0.0 86-87 3.9378605603575576E-4 3.029123507967352E-5 0.0 1.8074174147339597 0.0 88-89 3.9378605603575576E-4 3.029123507967352E-5 0.0 1.9994941363741694 0.0 90-91 4.240772911154293E-4 3.029123507967352E-5 0.0 2.225542478156233 0.0 92-93 4.240772911154293E-4 3.029123507967352E-5 0.0 2.470704589273571 0.0 94-95 4.240772911154293E-4 3.029123507967352E-5 0.0 2.7154577687173327 0.0 96-97 4.240772911154293E-4 3.029123507967352E-5 0.0 2.9643608473670104 0.0 98-99 5.300966138942866E-4 3.029123507967352E-5 0.0 3.2370122543191515 0.0 100-101 5.452422314341234E-4 3.029123507967352E-5 0.0 3.5633851666850935 0.0 102-103 6.058247015934704E-4 3.029123507967352E-5 0.0 3.915975143012494 0.0 104-105 8.17863347151185E-4 6.058247015934704E-5 0.0 4.271791135875879 0.0 106-107 8.481545822308586E-4 6.058247015934704E-5 0.0 4.630166738103496 0.0 108-109 8.481545822308586E-4 6.058247015934704E-5 0.0 5.005051063449535 0.0 110-111 8.481545822308586E-4 6.058247015934704E-5 0.0 5.444894942423935 0.0 112-113 8.481545822308586E-4 6.058247015934704E-5 0.0 5.924632377998265 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10590 0.0 40.55916 1 GTACATG 17635 0.0 35.740437 1 ATCAACG 12415 0.0 33.684246 3 TCAACGC 12435 0.0 33.582165 4 TACATGG 18220 0.0 33.25133 2 CAACGCA 12585 0.0 33.230747 5 ACATGGG 18420 0.0 32.275826 3 AACGCAG 12695 0.0 31.92775 6 TATCAAC 13760 0.0 31.257568 2 CGCATAG 460 0.0 31.043837 8 GGTATCA 7500 0.0 29.70708 1 CATGGGA 12595 0.0 29.040672 4 ACGCATA 545 0.0 27.297623 7 TACCTGG 3130 0.0 27.025919 2 ACGCAGA 14895 0.0 26.72803 7 CGCAGAG 14665 0.0 25.561155 8 GTACCTG 3495 0.0 24.374657 1 AACGCAT 640 0.0 24.179495 6 ATGGGAG 5495 0.0 23.634426 5 CGCAGAT 980 0.0 23.071697 8 >>END_MODULE