FastQCFastQC Report
Thu 26 May 2016
SRR1364622_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364622_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301285
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128470.3891514970685657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110130.3335973719324445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77170.23375746110984055No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110450.045.4259761
GTATCAA166150.044.50711
ATCAACG211950.034.138083
TCAACGC215250.033.5604364
TATCAAC219400.033.3546942
CAACGCA217500.032.9178775
AACGCAG222900.032.1898276
GTACATG180600.031.938571
TACATGG189750.030.3135662
ACATGGG191900.029.146863
ACGCAGA259100.027.4626357
CGCAGAG261750.027.1391018
CATGGGA129800.024.9846864
GAGTACT154350.024.42220512-13
GCAGAGT281900.024.3331939
AGTACTT164700.024.27952612-13
GTACTTT164200.022.95716914-15
ATGGGAT51200.022.4529765
CATGGGG65500.022.1666774
TACCTGG26350.022.1455842
Produced by FastQC (version 0.11.5)