##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364622_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3301285 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.64618534903833 31.0 30.0 33.0 26.0 34.0 2 29.733745193159635 31.0 30.0 34.0 26.0 34.0 3 29.836066259047612 31.0 30.0 34.0 26.0 34.0 4 33.55551701837315 35.0 33.0 37.0 28.0 37.0 5 32.91758754545578 35.0 33.0 37.0 26.0 37.0 6 32.954261749591446 35.0 33.0 37.0 26.0 37.0 7 32.75566241630153 35.0 33.0 37.0 25.0 37.0 8 32.691834846128096 35.0 33.0 37.0 25.0 37.0 9 33.988558394685704 38.0 34.0 39.0 25.0 39.0 10-11 33.79083387226489 38.0 33.0 39.0 23.5 39.0 12-13 33.66121434532311 37.0 33.0 39.0 22.5 39.0 14-15 34.644515241792206 39.0 33.0 41.0 22.5 41.0 16-17 34.5577428183268 38.5 33.0 41.0 21.5 41.0 18-19 34.61600422259817 39.0 33.5 41.0 22.0 41.0 20-21 34.63906812044401 39.0 34.0 41.0 22.0 41.0 22-23 34.62226163448475 39.0 34.0 41.0 22.0 41.0 24-25 34.613759187710244 39.0 34.0 41.0 22.5 41.0 26-27 34.51606601671773 39.0 33.5 41.0 22.0 41.0 28-29 34.276085978641646 39.0 33.0 41.0 19.0 41.0 30-31 34.19990443115333 38.0 33.0 41.0 19.5 41.0 32-33 34.00464819002298 38.0 33.0 41.0 18.5 41.0 34-35 33.862934281650936 38.0 33.0 41.0 18.0 41.0 36-37 33.68991998570254 38.0 33.0 40.0 16.0 41.0 38-39 33.549764864287695 38.0 33.0 40.0 15.5 41.0 40-41 33.3697926716415 38.0 32.5 40.0 14.5 41.0 42-43 33.17756858314263 38.0 32.0 40.0 9.0 41.0 44-45 32.956015460646384 38.0 32.0 40.0 8.5 41.0 46-47 32.66676672871321 38.0 31.5 40.0 4.5 41.0 48-49 32.385368424719466 38.0 31.0 40.0 2.0 41.0 50-51 32.18273702512809 38.0 31.0 40.0 2.0 41.0 52-53 32.01705669156101 37.5 30.5 40.0 2.0 41.0 54-55 31.805971159715078 37.0 30.0 40.0 2.0 41.0 56-57 31.59257334643934 37.0 30.0 40.0 2.0 41.0 58-59 31.412690512936628 37.0 30.0 40.0 2.0 41.0 60-61 31.20034728901019 36.0 29.5 40.0 2.0 41.0 62-63 30.940744891761845 36.0 29.0 40.0 2.0 41.0 64-65 30.6796218745125 35.5 29.0 39.0 2.0 41.0 66-67 30.404743304501125 35.0 29.0 39.0 2.0 41.0 68-69 30.097571097315136 35.0 29.0 39.0 2.0 40.0 70-71 29.842254909830565 35.0 28.5 38.0 2.0 40.0 72-73 29.686852846694546 35.0 29.0 37.5 2.0 40.0 74-75 29.3977676874308 35.0 29.0 37.0 2.0 39.0 76-77 29.100262625008142 34.5 28.0 36.5 2.0 39.0 78-79 28.793636720246813 34.0 28.0 36.0 2.0 39.0 80-81 28.517169374955508 34.0 28.0 36.0 2.0 37.5 82-83 28.259025500676252 34.0 28.0 35.0 2.0 37.0 84-85 28.228291710652066 34.0 28.0 35.0 2.0 37.0 86-87 28.07416475705672 34.0 28.0 35.0 2.0 36.0 88-89 27.924842296257367 34.0 28.0 35.0 2.0 36.0 90-91 27.76578983638189 34.0 27.0 35.0 2.0 36.0 92-93 27.632806013415987 34.0 27.0 35.0 2.0 35.5 94-95 27.545277369266817 34.0 27.0 35.0 2.0 35.0 96-97 27.462470371385688 34.0 27.0 35.0 2.0 35.0 98-99 27.37399133973589 34.0 27.0 35.0 2.0 35.0 100-101 27.28038248742535 34.0 26.5 35.0 2.0 35.0 102-103 26.10001484270519 32.0 24.5 34.0 2.0 35.0 104-105 27.027434620155486 34.0 25.5 35.0 2.0 35.0 106-107 27.201962872033164 34.0 27.0 35.0 2.0 35.0 108-109 27.214574779214757 34.0 27.0 35.0 2.0 35.0 110-111 27.153312876652578 34.0 27.0 35.0 2.0 35.0 112-113 27.107851639588826 34.0 27.0 35.0 2.0 35.0 114-115 27.02894191201305 34.0 26.0 35.0 2.0 35.0 116-117 26.91803691592819 34.0 25.5 35.0 2.0 35.0 118-119 26.818974581110083 34.0 25.0 35.0 2.0 35.0 120-121 26.68612691724586 34.0 25.0 35.0 2.0 35.0 122-123 26.50850941375858 34.0 25.0 35.0 2.0 35.0 124-125 25.709621102086007 33.0 22.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6383977131332763 1101 2 0.5899738876422802 1101 3 0.5056833841650956 1101 4 0.5485830326930383 1101 5 0.8737197860675892 1101 6 0.8263085978177855 1101 7 0.7505887990476054 1101 8 0.7781576723733465 1101 9 0.9639239793997163 1101 10-11 1.054797666445154 1101 12-13 1.0206268822778597 1101 14-15 1.1482026033053998 1101 16-17 1.2349926103156506 1101 18-19 1.2251902470956537 1101 20-21 1.220797042005131 1101 22-23 1.1245796048233174 1101 24-25 1.0412255386273017 1101 26-27 1.1514661392528467 1101 28-29 1.2981917095929916 1101 30-31 1.2459983895964086 1101 32-33 1.3108516625789761 1101 34-35 1.2285921454548543 1101 36-37 1.1848426385984894 1101 38-39 1.2804394742633747 1101 40-41 1.2962431207329956 1101 42-43 1.1912429186818372 1101 44-45 0.9874426569419938 1101 46-47 1.0773156092837723 1101 48-49 1.1826240979269542 1101 50-51 1.2229282238578278 1101 52-53 1.177793756515193 1101 54-55 1.1511945013410205 1101 56-57 1.1677980263916474 1101 58-59 1.1969710664864515 1101 60-61 1.1120428821676356 1101 62-63 1.1380329214055926 1101 64-65 1.1830308993380676 1101 66-67 1.1562523020785207 1101 68-69 1.20168341537655 1101 70-71 1.1378278321146063 1101 72-73 1.1487760218176675 1101 74-75 1.077401833008814 1101 76-77 1.0990096319409375 1101 78-79 0.9963223853707817 1101 80-81 1.0314921170787805 1101 82-83 0.9593878836321394 1101 84-85 0.9870134985567915 1101 86-87 0.9989004560789496 1101 88-89 1.0094931971097623 1101 90-91 0.966510909254712 1101 92-93 0.9620998350435315 1101 94-95 0.9343809123286064 1101 96-97 0.9632719917884884 1101 98-99 0.9002746338227929 1101 100-101 0.9754937821907745 1101 102-103 0.9667874717102833 1101 104-105 0.9090254900491068 1101 106-107 0.9122362244228484 1101 108-109 0.8873894841460093 1101 110-111 0.8656398213863881 1101 112-113 0.8661614286619468 1101 114-115 0.8444285974260985 1101 116-117 0.9464459050887086 1101 118-119 0.9907610578768065 1101 120-121 1.0066986488977783 1101 122-123 1.0413703340150242 1101 124-125 0.8838479718533954 1102 1 0.47673216645276284 1102 2 -0.5441766537692097 1102 3 0.05029306899081121 1102 4 0.49739419284596664 1102 5 0.7797572750586355 1102 6 0.6921012934717297 1102 7 0.6299088910901034 1102 8 0.5755629953620769 1102 9 0.809079148390353 1102 10-11 0.8774752224317268 1102 12-13 0.991899619269482 1102 14-15 1.1014865808853145 1102 16-17 1.125216722090272 1102 18-19 1.138356575221053 1102 20-21 1.0409136478361845 1102 22-23 1.0908794760249165 1102 24-25 1.1276376248051534 1102 26-27 1.1020961950994277 1102 28-29 1.1297949188624088 1102 30-31 1.0770958869364478 1102 32-33 0.9570129100399143 1102 34-35 0.8688323570304206 1102 36-37 0.8610615780734179 1102 38-39 0.8050135613473941 1102 40-41 0.8491431848776543 1102 42-43 0.7693510711628591 1102 44-45 0.5316172016930807 1102 46-47 0.5465063174713833 1102 48-49 0.664652901934474 1102 50-51 0.6300788263594725 1102 52-53 0.7062946041410711 1102 54-55 0.6368972417434939 1102 56-57 0.5968131767497873 1102 58-59 0.6531892805158073 1102 60-61 0.46941830916235716 1102 62-63 0.6493633631383098 1102 64-65 0.6089373244949918 1102 66-67 0.6523004691193641 1102 68-69 0.48657741123260223 1102 70-71 0.33918390625768424 1102 72-73 0.3940202169803477 1102 74-75 0.4441530713571389 1102 76-77 0.5302264459876014 1102 78-79 0.5382287672555144 1102 80-81 0.5405214327163286 1102 82-83 0.4615593840572245 1102 84-85 0.5594509575118494 1102 86-87 0.4712744067282877 1102 88-89 0.6182574705696524 1102 90-91 0.5477601774455785 1102 92-93 0.5879142431563054 1102 94-95 0.3997940217665281 1102 96-97 0.39724180816166665 1102 98-99 0.44103022993542496 1102 100-101 0.2526418010879965 1102 102-103 0.28212930930211044 1102 104-105 0.2262725131768235 1102 106-107 0.49021793301263017 1102 108-109 0.5318128440026548 1102 110-111 0.42287938642449063 1102 112-113 0.4963955184950173 1102 114-115 0.5660166487011047 1102 116-117 0.6220328160141726 1102 118-119 0.5623896450142745 1102 120-121 0.46598772496011165 1102 122-123 0.4819660775585568 1102 124-125 0.35927623928572316 1103 1 0.2956559118667492 1103 2 -1.00510999282805 1103 3 0.0044636639330910555 1103 4 0.251867727003642 1103 5 0.5655193883010767 1103 6 0.6083394122405679 1103 7 0.6650092915125114 1103 8 0.7164308414407614 1103 9 0.8737489371682301 1103 10-11 0.9485646734601545 1103 12-13 0.9843591887411591 1103 14-15 1.0437433327659136 1103 16-17 1.213666021537989 1103 18-19 1.190216542957387 1103 20-21 1.1710572961429833 1103 22-23 1.1267203043265326 1103 24-25 0.9924605495700476 1103 26-27 1.0267335706630831 1103 28-29 1.1607005511604243 1103 30-31 1.0517565384010368 1103 32-33 1.0404336748781162 1103 34-35 1.12842342754454 1103 36-37 1.1208614160073438 1103 38-39 1.0862359018540673 1103 40-41 1.1283903317905697 1103 42-43 1.029455236961624 1103 44-45 1.1585500788657512 1103 46-47 1.0853757901926429 1103 48-49 1.0534531665042337 1103 50-51 1.0351100314486885 1103 52-53 1.0365762414741297 1103 54-55 1.0281371497337268 1103 56-57 1.0759703391792605 1103 58-59 1.0037247757840326 1103 60-61 1.036204805593421 1103 62-63 1.119564655351379 1103 64-65 1.0678311007177719 1103 66-67 1.055088855822767 1103 68-69 0.926594271573137 1103 70-71 0.9801007182518582 1103 72-73 1.023552902263031 1103 74-75 1.008513020400116 1103 76-77 0.8898605859980755 1103 78-79 0.886880527518219 1103 80-81 0.8975495477400841 1103 82-83 0.8877528801787271 1103 84-85 0.859091724584701 1103 86-87 0.8149645439204427 1103 88-89 0.8187481338249434 1103 90-91 0.8185207535599126 1103 92-93 0.8087371269026349 1103 94-95 0.7328706685925965 1103 96-97 0.7405870261977903 1103 98-99 0.7870552422890604 1103 100-101 0.8172884955575626 1103 102-103 0.8591888206214193 1103 104-105 0.800694445121465 1103 106-107 0.8013777394306274 1103 108-109 0.7750048405964023 1103 110-111 0.7835719763988251 1103 112-113 0.7914767085547645 1103 114-115 0.758107542429471 1103 116-117 0.7360221131588744 1103 118-119 0.847368517247201 1103 120-121 0.8277350262267511 1103 122-123 0.8954042731517511 1103 124-125 0.8849376690349082 1104 1 0.36912369878920614 1104 2 -0.5853564384924042 1104 3 0.2520963683440378 1104 4 0.4115445911138593 1104 5 0.7474929554686796 1104 6 0.8092876538767584 1104 7 0.7635430390646363 1104 8 0.853437434990937 1104 9 0.8603106838559569 1104 10-11 0.9781590093057133 1104 12-13 0.9088553961096508 1104 14-15 0.9717934779211816 1104 16-17 1.1605872306235625 1104 18-19 1.0731906595875031 1104 20-21 1.1098763202774578 1104 22-23 1.015242652206659 1104 24-25 1.0130343819203205 1104 26-27 0.8880794481028005 1104 28-29 1.2264906464285161 1104 30-31 1.1398615753641153 1104 32-33 1.1882000385586409 1104 34-35 1.1744427768335086 1104 36-37 1.1560400625203542 1104 38-39 1.2246328198384049 1104 40-41 1.3083003573037217 1104 42-43 1.3977594582744004 1104 44-45 1.2705675820946851 1104 46-47 1.3331518699786216 1104 48-49 1.3623908737043777 1104 50-51 1.2526293003285005 1104 52-53 1.0397932774924001 1104 54-55 1.2744302670456484 1104 56-57 1.3713635859834135 1104 58-59 1.275944294434776 1104 60-61 1.1958106625623017 1104 62-63 1.238465705193331 1104 64-65 1.291976816334067 1104 66-67 1.2697935113449503 1104 68-69 1.2521426452143487 1104 70-71 1.25145652957179 1104 72-73 1.2604057970761424 1104 74-75 1.2651870844292503 1104 76-77 1.2218721590522996 1104 78-79 1.192590498420163 1104 80-81 1.1819456917863747 1104 82-83 1.1789636158051735 1104 84-85 1.193707842095975 1104 86-87 1.21148894223386 1104 88-89 1.2158716849944788 1104 90-91 1.2676050106212564 1104 92-93 1.2648636967656195 1104 94-95 1.2000456229855345 1104 96-97 1.2034104426819994 1104 98-99 1.1857670626014176 1104 100-101 1.209076606562391 1104 102-103 1.1521491671848487 1104 104-105 1.2128026180413407 1104 106-107 1.2374180202260767 1104 108-109 1.166455023778493 1104 110-111 1.1097925830858522 1104 112-113 1.1524969708226038 1104 114-115 1.2050159991266334 1104 116-117 1.1116116336690673 1104 118-119 1.0771877474301164 1104 120-121 1.0451713312923623 1104 122-123 1.111685890280242 1104 124-125 1.0676470217915188 1105 1 0.6292286438068011 1105 2 0.3984383932138513 1105 3 0.7360081977966182 1105 4 0.6185851756058369 1105 5 0.8562459555784869 1105 6 0.7965652927968776 1105 7 1.0320123425162677 1105 8 1.0433512488298788 1105 9 1.1470849056575432 1105 10-11 1.2373908831520808 1105 12-13 1.2393384072525393 1105 14-15 1.292480052070303 1105 16-17 1.417756610679298 1105 18-19 1.4454235648441696 1105 20-21 1.5462256245657215 1105 22-23 1.378315091459335 1105 24-25 1.4538544380792473 1105 26-27 1.391970113382044 1105 28-29 1.5648262325676185 1105 30-31 1.4510085846664111 1105 32-33 1.4796432221000018 1105 34-35 1.5349022119500901 1105 36-37 1.4216965179200258 1105 38-39 1.2950716725022957 1105 40-41 1.2453848192186712 1105 42-43 1.3668379016684042 1105 44-45 1.3435947647889463 1105 46-47 1.323556420733425 1105 48-49 1.344505088342295 1105 50-51 1.3872669079795017 1105 52-53 1.298900473572779 1105 54-55 1.2902399960291895 1105 56-57 1.2212718179187014 1105 58-59 1.1339103376363475 1105 60-61 1.1555719348703022 1105 62-63 1.1817095428762805 1105 64-65 1.136939442417109 1105 66-67 1.093832985992389 1105 68-69 1.1364581822536124 1105 70-71 1.1105670591075132 1105 72-73 1.0226972600316486 1105 74-75 1.0817030810931776 1105 76-77 1.1083660437957938 1105 78-79 1.1269412001451933 1105 80-81 0.8992992354454863 1105 82-83 0.9697139635303174 1105 84-85 0.8144125868448029 1105 86-87 0.8150966559802448 1105 88-89 0.9429966647322985 1105 90-91 0.9478294175276538 1105 92-93 0.7876535831928351 1105 94-95 0.8525987556492147 1105 96-97 0.9193041289868589 1105 98-99 1.05962682478879 1105 100-101 1.0577980303530659 1105 102-103 0.6909773857340262 1105 104-105 0.5933810901945691 1105 106-107 0.9119928739462111 1105 108-109 0.9580616119934895 1105 110-111 0.8760678519845833 1105 112-113 0.6655479387011702 1105 114-115 0.8418795052418879 1105 116-117 0.5910829411156193 1105 118-119 0.5395834298864663 1105 120-121 0.4798865888442698 1105 122-123 0.4809806161002719 1105 124-125 0.3122371832614803 1106 1 0.4821174948294136 1106 2 0.48970515088604216 1106 3 0.6151909374337805 1106 4 0.5117190349713283 1106 5 0.5715080274568294 1106 6 0.6786264272003137 1106 7 0.7007198891671393 1106 8 0.675967916307961 1106 9 0.6755644521769 1106 10-11 0.8012855051113803 1106 12-13 0.6802819772950741 1106 14-15 0.8688591319071008 1106 16-17 0.9814967598267685 1106 18-19 0.9275486223022043 1106 20-21 0.9698013765467906 1106 22-23 0.808284769106308 1106 24-25 0.8331757646059188 1106 26-27 0.921842286241116 1106 28-29 1.1014468795877974 1106 30-31 0.8997311194083721 1106 32-33 0.8779146164066205 1106 34-35 0.8509218328819443 1106 36-37 0.9256072210167261 1106 38-39 1.0633013216083498 1106 40-41 1.0263542074415284 1106 42-43 0.9943627951457898 1106 44-45 0.9189552092533404 1106 46-47 0.8962308797742935 1106 48-49 0.8430634408915338 1106 50-51 0.8715664794863827 1106 52-53 0.924977752499224 1106 54-55 0.9669502143602493 1106 56-57 0.850936232946875 1106 58-59 0.82345649975559 1106 60-61 0.910456144674491 1106 62-63 0.9906405649370065 1106 64-65 0.852010488055388 1106 66-67 0.7686601890457752 1106 68-69 0.7925561199921809 1106 70-71 0.8834617950344814 1106 72-73 0.8945739177790131 1106 74-75 0.8445269128535173 1106 76-77 0.9060616287423251 1106 78-79 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72-73 -2.805884033860078 2216 74-75 -2.5307538453332263 2216 76-77 -2.7541345844289715 2216 78-79 -2.422483744088904 2216 80-81 -2.0794213125009158 2216 82-83 -2.0331385238891144 2216 84-85 -2.0005945778395002 2216 86-87 -2.092025718113284 2216 88-89 -1.9626412726801519 2216 90-91 -1.906277744322331 2216 92-93 -2.022878995813006 2216 94-95 -1.9051523143782703 2216 96-97 -1.8412701539164864 2216 98-99 -1.8374636398696218 2216 100-101 -2.0375321375553455 2216 102-103 -1.852793298201128 2216 104-105 -1.8449720353843482 2216 106-107 -1.9434020025740182 2216 108-109 -2.0014268209275947 2216 110-111 -1.933115943059864 2216 112-113 -1.915601004770874 2216 114-115 -1.9583226662535402 2216 116-117 -2.039901465907409 2216 118-119 -2.2267779901586344 2216 120-121 -2.1985572954899055 2216 122-123 -2.151732796965984 2216 124-125 -2.207770698037347 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 120410.0 3 38994.0 4 18063.0 5 14055.0 6 15369.0 7 18357.0 8 20425.0 9 21967.0 10 23941.0 11 26792.0 12 30692.0 13 34843.0 14 39369.0 15 32193.0 16 20543.0 17 17517.0 18 15962.0 19 14883.0 20 14721.0 21 15156.0 22 17644.0 23 23041.0 24 31544.0 25 42233.0 26 51017.0 27 56737.0 28 66056.0 29 76586.0 30 89165.0 31 105273.0 32 127848.0 33 158964.0 34 205375.0 35 281628.0 36 441343.0 37 689012.0 38 282620.0 39 947.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.74144365917573 20.506182096802842 13.737093732553465 24.01528051146796 2 15.251630192591605 21.18971470492832 38.744926016085174 24.813729086394904 3 18.488073583468257 26.070969334668163 29.938190734819926 25.502766347043654 4 12.660002896062265 17.30606228230306 36.93448779988331 33.09944702175137 5 12.576003509940337 39.327390715080654 34.09462840354981 14.001977371429195 6 30.9849628114169 38.0722095234518 17.10147754720742 13.841350117923884 7 27.77086127700616 33.62128190122389 20.96765985598331 17.640196965786643 8 26.095384070142387 36.27369342543888 19.231178162442806 18.399744341975925 9 26.475285194138 16.08288956337883 20.112411624652403 37.32941361783077 10-11 24.439162491140124 27.732694770638904 27.065974455976743 20.762168282244225 12-13 25.468178976717187 24.97950214097165 28.121290372224156 21.431028510087007 14-15 22.489838422011264 26.05933740528546 26.45860581711053 24.992218355592748 16-17 21.44568669519442 29.387278650525523 27.130936205950217 22.03609844832984 18-19 21.313305549164937 27.505236225761486 30.178683695614474 21.0027745294591 20-21 22.269050825578816 26.558376867827864 30.31374676170463 20.858825544888685 22-23 21.89275812661861 26.420782670484456 30.187734095800028 21.49872510709691 24-25 21.530298050607566 26.61890748602438 30.45857900787118 21.392215455496874 26-27 21.32880075485758 26.89560277285966 30.2896296442143 21.485966828068463 28-29 21.252902123594513 26.79059567237512 30.428484597851778 21.52801760617859 30-31 21.408363926223263 26.662970125719248 30.52854123902378 21.40012470903371 32-33 21.23337498029103 26.71855042535786 30.3443045727996 21.703770021551506 34-35 21.580581500839823 26.654196774892203 30.324191943440205 21.44102978082777 36-37 21.632318126254344 26.470334768584493 30.07652636605705 21.820820739104114 38-39 21.488799058548413 26.797201695703336 30.149502390735726 21.564496855012518 40-41 21.60979739707417 26.58289120751465 30.22179242325337 21.58551897215781 42-43 21.479426766960387 26.609561669111002 29.908527899422342 22.002483664506272 44-45 21.644980666619208 26.752067755434627 29.72156296714764 21.88138861079852 46-47 21.86459022143815 27.064841154181572 29.010009138871162 22.060559485509117 48-49 21.852347338450233 27.274726496362096 28.729176836328556 22.143749328859112 50-51 22.093529578634204 27.396145097207096 28.48008944418924 22.03023587996946 52-53 22.203983513674917 27.36635757962476 28.351156967377666 22.078501939322653 54-55 22.053442826051068 27.32131579066939 28.451708955755105 22.173532427524435 56-57 22.02587459041794 27.543619334726955 28.289113969910012 22.141392104945094 58-59 22.05911730997959 27.15651351966591 28.4176442283736 22.3667249419809 60-61 22.007400148729964 27.36019459089415 28.573494866392934 22.05891039398295 62-63 22.077160863118454 27.241407512529214 28.741474910527266 21.93995671382507 64-65 21.91392345554182 27.087915533138602 28.700809476107835 22.29735153521174 66-67 21.88291831817005 27.412356097701352 28.52495316217776 22.179772421950847 68-69 21.929100489994134 27.180619302182436 28.69725050768433 22.193029700139096 70-71 21.862714064371904 27.0871191066509 28.707987950146684 22.342178878830516 72-73 22.04081793385204 27.291769816648113 28.296988946966735 22.37042330253311 74-75 21.899733989743392 27.50214051696836 28.275149995221415 22.322975498066832 76-77 21.937501959472282 27.286607599526423 28.54571337134798 22.230177069653315 78-79 21.925170703735738 27.288288821800037 28.47441151644955 22.312128958014675 80-81 21.866999673606752 27.48827899882847 28.333781398311086 22.310939929253696 82-83 22.085157562345884 27.40956122934722 28.227304526228536 22.277976682078364 84-85 21.888163446806423 27.333911492565615 28.261611701151253 22.51631335947671 86-87 21.9259605478745 27.372823827942423 28.336893077718344 22.364322546464734 88-89 22.00403782163612 27.265807102385892 28.286909491304147 22.443245584673846 90-91 21.903289173761127 27.353939450850202 28.400486477235383 22.342284898153295 92-93 21.916969762421296 27.32539056601116 28.298619131437352 22.45902054013019 94-95 21.758061630957947 27.22031199140767 28.330219962560786 22.691406415073594 96-97 22.005234011319256 27.10694098726455 28.224189444673154 22.66363555674304 98-99 21.72653382375103 27.144634598680657 28.140009859849286 22.988821717719027 100-101 21.577866922002976 27.246417814735977 28.369047399074354 22.80666786418669 102-103 21.79519101827442 26.946797318980114 28.336757761608293 22.921253901137163 104-105 21.709734655348807 26.894053732310795 28.44565239615936 22.950559216181034 106-107 21.66868691336042 26.990460487503725 28.308555965629704 23.032296633506146 108-109 21.725032504001657 27.009174428650535 27.99218571084101 23.273607356506798 110-111 21.623270455968807 27.009595556858084 28.19780584275585 23.16932814441726 112-113 21.55917281289417 26.96661105072925 28.427513894896784 23.046702241479796 114-115 21.45523863067368 27.118372215808872 28.047680394805276 23.37870875871217 116-117 21.365471360730687 27.065244686886874 28.168246335671764 23.401037616710678 118-119 21.191227783733453 27.030834767894863 28.24045563471434 23.537481813657347 120-121 21.178590671010298 27.18124053390481 28.21511724514837 23.425051549936526 122-123 21.17140763569293 27.065189371691222 28.09637171757805 23.667031275037793 124-125 21.154915105653334 26.934958307850337 28.10493450756553 23.805192078930794 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 259.0 1 213.0 2 382.0 3 758.0 4 638.5 5 527.5 6 1397.5 7 3185.5 8 4886.0 9 5475.0 10 4478.5 11 3219.5 12 2919.5 13 3075.5 14 3438.5 15 3815.0 16 4099.5 17 4393.0 18 4823.5 19 5214.5 20 5806.5 21 6781.0 22 7913.5 23 9496.0 24 11484.5 25 14137.0 26 18176.5 27 23177.0 28 27724.0 29 33175.0 30 40688.0 31 48685.0 32 58472.5 33 67967.5 34 76519.5 35 84379.5 36 92889.0 37 101299.5 38 104586.0 39 107199.5 40 108962.5 41 110674.0 42 112491.5 43 115540.0 44 121745.5 45 124726.5 46 123537.5 47 121454.5 48 122053.0 49 119163.0 50 114220.5 51 109477.5 52 103038.0 53 99489.5 54 97034.0 55 90706.0 56 81457.0 57 73546.5 58 67811.5 59 61177.0 60 50855.5 61 40938.5 62 33242.5 63 25517.0 64 19210.5 65 14911.5 66 10979.5 67 8046.5 68 5867.5 69 4061.5 70 2865.0 71 2099.5 72 1593.5 73 1426.0 74 1141.5 75 799.5 76 564.0 77 346.5 78 260.5 79 176.0 80 132.0 81 98.0 82 70.5 83 52.0 84 29.0 85 21.5 86 11.0 87 10.5 88 8.5 89 6.0 90 5.0 91 1.5 92 1.0 93 2.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05812888011789348 2 0.07860575503175278 3 0.0 4 0.007603100004998053 5 0.028594925915211802 6 9.087370523902057E-5 7 3.0291235079673523E-5 8 0.0 9 7.57280876991838E-4 10-11 0.060597615776886875 12-13 0.0100112531938321 14-15 0.007497080682219197 16-17 0.05476655302404972 18-19 1.3631055785853086E-4 20-21 0.010859407776062957 22-23 0.01270717311592304 24-25 0.0 26-27 0.0 28-29 0.0533580105928449 30-31 1.5145617539836761E-5 32-33 0.00374096753233968 34-35 0.0 36-37 1.3631055785853086E-4 38-39 0.0 40-41 0.0 42-43 0.003468346416622618 44-45 0.0 46-47 6.0582470159347046E-5 48-49 1.0601932277885732E-4 50-51 2.574754981772249E-4 52-53 1.0601932277885732E-4 54-55 0.0 56-57 0.0016357266943023703 58-59 4.543685261951028E-5 60-61 0.0 62-63 0.0 64-65 4.2407729111542926E-4 66-67 0.0 68-69 6.361159366731439E-4 70-71 0.0 72-73 0.001953784662638942 74-75 0.002923104185188495 76-77 4.0893167357559257E-4 78-79 7.421352594520012E-4 80-81 0.0015902898416828596 82-83 1.3631055785853086E-4 84-85 9.087370523902057E-5 86-87 5.452422314341234E-4 88-89 0.0 90-91 0.0 92-93 0.006376304984271277 94-95 0.006073392633474541 96-97 0.005573587254659928 98-99 5.300966138942866E-4 100-101 5.452422314341234E-4 102-103 1.2116494031869409E-4 104-105 3.78640438495919E-4 106-107 0.003650093827100659 108-109 0.004634558967190048 110-111 5.300966138942866E-4 112-113 0.002211260160816167 114-115 3.6349482095608226E-4 116-117 0.0019992215152584525 118-119 0.0013328143435056348 120-121 0.010889699011142631 122-123 7.875721120715116E-4 124-125 0.0019386390450991055 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3301285.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.06785439306066 #Duplication Level Percentage of deduplicated Percentage of total 1 72.59086917915198 33.44105591610887 2 12.790210363770136 11.78435097389556 3 5.32555346323802 7.3601046452072705 4 2.820718209269253 5.19777742993883 5 1.6143136206668494 3.7183982410807483 6 1.0578951551045304 2.9240975981087893 7 0.7138217204321814 2.3018964555631647 8 0.5380454452347269 1.9829279382338312 9 0.37832594211152104 1.568579797287994 >10 1.9446545211036743 16.084105092546 >50 0.1428038779923553 4.549171784828792 >100 0.07788190129441624 6.3781193698755585 >500 0.003912019422097899 1.2008039703422912 >1k 7.956649666358613E-4 0.544076116903118 >5k 6.630541388632178E-5 0.23571948091968115 >10k+ 1.3261082777264355E-4 0.7288151891594651 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12847 0.3891514970685657 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11013 0.3335973719324445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7717 0.23375746110984055 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.029123507967352E-5 0.0 0.0 0.0 0.0 3 3.029123507967352E-5 0.0 0.0 0.0 0.0 4 3.029123507967352E-5 0.0 0.0 0.0 0.0 5 3.029123507967352E-5 0.0 0.0 0.0 0.0 6 3.029123507967352E-5 0.0 0.0 0.0 0.0 7 3.029123507967352E-5 0.0 0.0 3.029123507967352E-5 0.0 8 3.029123507967352E-5 0.0 0.0 3.029123507967352E-5 0.0 9 3.029123507967352E-5 0.0 0.0 3.029123507967352E-5 0.0 10-11 3.029123507967352E-5 0.0 0.0 4.5436852619510276E-5 0.0 12-13 3.029123507967352E-5 0.0 0.0 6.058247015934704E-5 0.0 14-15 3.029123507967352E-5 0.0 0.0 9.087370523902057E-5 0.0 16-17 3.029123507967352E-5 0.0 0.0 9.087370523902057E-5 0.0 18-19 6.058247015934704E-5 0.0 0.0 1.514561753983676E-4 0.0 20-21 9.087370523902057E-5 0.0 0.0 1.6660179293820435E-4 0.0 22-23 1.2116494031869408E-4 0.0 0.0 2.8776673325689846E-4 0.0 24-25 1.514561753983676E-4 0.0 0.0 6.058247015934704E-4 0.0 26-27 1.514561753983676E-4 0.0 0.0 7.57280876991838E-4 0.0 28-29 1.8174741047804113E-4 3.029123507967352E-5 0.0 0.0013025231084259614 0.0 30-31 1.8174741047804113E-4 3.029123507967352E-5 0.0 0.0033471814763039237 0.0 32-33 1.8174741047804113E-4 3.029123507967352E-5 0.0 0.006664071717528175 0.0 34-35 2.1203864555771466E-4 3.029123507967352E-5 0.0 0.011859018533692183 0.0 36-37 3.029123507967352E-4 3.029123507967352E-5 0.0 0.0204768749138593 0.0 38-39 3.332035858764087E-4 3.029123507967352E-5 0.0 0.036349482095608225 0.0 40-41 3.332035858764087E-4 3.029123507967352E-5 0.0 0.056220532307874056 0.0 42-43 3.483492034162455E-4 3.029123507967352E-5 0.0 0.07460731200123588 0.0 44-45 3.6349482095608226E-4 3.029123507967352E-5 0.0 0.09559913791144964 0.0 46-47 3.9378605603575576E-4 3.029123507967352E-5 0.0 0.1224068809569607 0.0 48-49 3.9378605603575576E-4 3.029123507967352E-5 0.0 0.15315248456282932 0.0 50-51 3.9378605603575576E-4 3.029123507967352E-5 0.0 0.18769963817119698 0.0 52-53 3.9378605603575576E-4 3.029123507967352E-5 0.0 0.21967203679779237 0.0 54-55 3.9378605603575576E-4 3.029123507967352E-5 0.0 0.2537951131150446 0.0 56-57 3.9378605603575576E-4 3.029123507967352E-5 0.0 0.29490031911816156 0.0 58-59 3.9378605603575576E-4 3.029123507967352E-5 0.0 0.34439619723834813 0.0 60-61 3.9378605603575576E-4 3.029123507967352E-5 0.0 0.400541001458523 0.0 62-63 3.9378605603575576E-4 3.029123507967352E-5 0.0 0.455186389542254 0.0 64-65 3.9378605603575576E-4 3.029123507967352E-5 0.0 0.5133455608952271 0.0 66-67 3.9378605603575576E-4 3.029123507967352E-5 0.0 0.5783505513762066 0.0 68-69 3.9378605603575576E-4 3.029123507967352E-5 0.0 0.6559869868854098 0.0 70-71 3.9378605603575576E-4 3.029123507967352E-5 0.0 0.749571757664061 0.0 72-73 3.9378605603575576E-4 3.029123507967352E-5 0.0 0.8431262372076328 0.0 74-75 3.9378605603575576E-4 3.029123507967352E-5 0.0 0.9383770259156662 0.0 76-77 3.9378605603575576E-4 3.029123507967352E-5 0.0 1.0438208152280097 0.0 78-79 3.9378605603575576E-4 3.029123507967352E-5 0.0 1.1705896340364434 0.0 80-81 4.543685261951028E-4 3.029123507967352E-5 0.0 1.316487367797691 0.0 82-83 5.149509963544498E-4 3.029123507967352E-5 0.0 1.4720480055493543 0.0 84-85 5.452422314341234E-4 3.029123507967352E-5 0.0 1.6291989331427006 0.0 86-87 5.755334665137969E-4 3.029123507967352E-5 0.0 1.7960279103440024 0.0 88-89 5.755334665137969E-4 3.029123507967352E-5 0.0 1.986741526405627 0.0 90-91 7.269896419121645E-4 3.029123507967352E-5 0.0 2.2100182201779006 0.0 92-93 7.269896419121645E-4 3.029123507967352E-5 0.0 2.452863051811643 0.0 94-95 7.269896419121645E-4 3.029123507967352E-5 0.0 2.69529895177181 0.0 96-97 7.269896419121645E-4 3.029123507967352E-5 0.0 2.9414758192643164 0.0 98-99 7.724264945316748E-4 3.029123507967352E-5 0.0 3.211825092350403 0.0 100-101 8.17863347151185E-4 3.029123507967352E-5 0.0 3.5367894622851406 0.0 102-103 8.481545822308586E-4 3.029123507967352E-5 0.0 3.8882283716795127 0.0 104-105 8.481545822308586E-4 3.029123507967352E-5 0.0 4.242045143027639 0.0 106-107 9.238826699300424E-4 3.029123507967352E-5 0.0 4.598981911588972 0.0 108-109 9.390282874698791E-4 3.029123507967352E-5 0.0 4.9723365295634885 0.0 110-111 9.693195225495526E-4 3.029123507967352E-5 0.0 5.4131042912078176 0.0 112-113 0.0010299019927088996 3.029123507967352E-5 0.0 5.890236680565295 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11045 0.0 45.425976 1 GTATCAA 16615 0.0 44.5071 1 ATCAACG 21195 0.0 34.13808 3 TCAACGC 21525 0.0 33.560436 4 TATCAAC 21940 0.0 33.354694 2 CAACGCA 21750 0.0 32.917877 5 AACGCAG 22290 0.0 32.189827 6 GTACATG 18060 0.0 31.93857 1 TACATGG 18975 0.0 30.313566 2 ACATGGG 19190 0.0 29.14686 3 ACGCAGA 25910 0.0 27.462635 7 CGCAGAG 26175 0.0 27.139101 8 CATGGGA 12980 0.0 24.984686 4 GAGTACT 15435 0.0 24.422205 12-13 GCAGAGT 28190 0.0 24.333193 9 AGTACTT 16470 0.0 24.279526 12-13 GTACTTT 16420 0.0 22.957169 14-15 ATGGGAT 5120 0.0 22.452976 5 CATGGGG 6550 0.0 22.166677 4 TACCTGG 2635 0.0 22.145584 2 >>END_MODULE