FastQCFastQC Report
Thu 26 May 2016
SRR1364621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3636140
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36650.10079369881247696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG121150.030.83092
GTACATG127250.030.4301191
ACATGGG129250.028.1152113
GTATCAA167500.022.121961
CATGGGG74700.021.9298764
GAGTACA93950.021.0518051
AGAGTAC110650.020.88847710-11
GTGTAGC22450.019.9018291
CATGGGA62000.019.6963734
TCAACGC187300.019.4642524
CAACGCA186550.019.4166535
ATCAACG187750.019.3232153
TATCAAC190650.019.3105092
AACGCAG188300.019.0282526
AGGACCG12100.018.7095895
AGTACAT96450.018.5911542
TGTACCG7700.017.795215
GGACCGA9500.017.5423266
GAGTACT61250.017.53179212-13
ACGCAGA203950.017.1849617
Produced by FastQC (version 0.11.5)