##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364621_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3636140 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.568477561370024 30.0 23.0 31.0 2.0 33.0 2 24.432305961816652 30.0 22.0 31.0 2.0 34.0 3 24.992985693620156 30.0 24.0 31.0 2.0 34.0 4 28.115332192929866 35.0 26.0 37.0 2.0 37.0 5 28.327135643842098 35.0 27.0 37.0 2.0 37.0 6 28.368452259813978 35.0 27.0 37.0 2.0 37.0 7 28.103018310626105 35.0 26.0 37.0 2.0 37.0 8 27.85537493055823 35.0 26.0 37.0 2.0 37.0 9 28.74983223968274 35.0 25.0 39.0 2.0 39.0 10-11 28.631114313530283 35.0 24.5 39.0 2.0 39.0 12-13 28.615296853256474 35.0 25.0 39.0 2.0 39.0 14-15 29.10345489997635 36.0 24.0 40.0 2.0 41.0 16-17 29.465552068952242 36.0 25.5 40.0 2.0 41.0 18-19 29.273882881297197 36.0 24.5 40.0 2.0 41.0 20-21 29.156023145423443 36.0 24.0 40.0 2.0 41.0 22-23 29.192881599718383 36.0 24.5 40.0 2.0 41.0 24-25 29.248241266837912 36.0 25.0 40.0 2.0 41.0 26-27 29.011110683307024 36.0 24.0 40.0 2.0 41.0 28-29 28.879606670810254 36.0 24.0 40.0 2.0 41.0 30-31 28.718194293949075 36.0 23.5 40.0 2.0 41.0 32-33 28.591051774684143 35.0 23.0 40.0 2.0 41.0 34-35 28.614068215195235 35.0 23.5 40.0 2.0 41.0 36-37 28.54977998646917 35.0 23.0 40.0 2.0 41.0 38-39 28.25595865395722 35.0 21.5 40.0 2.0 41.0 40-41 28.104143679836312 35.0 20.5 40.0 2.0 41.0 42-43 27.601440401084666 34.5 16.5 39.5 2.0 41.0 44-45 27.43841986282156 34.0 16.0 39.0 2.0 41.0 46-47 27.382374303519665 34.0 17.0 39.0 2.0 40.0 48-49 27.298097708009045 34.0 15.5 39.0 2.0 40.0 50-51 27.058507235694996 34.0 14.0 39.0 2.0 40.0 52-53 26.948568811981936 34.0 13.0 39.0 2.0 40.0 54-55 26.767036060217706 33.5 11.0 39.0 2.0 40.0 56-57 26.527410660755635 33.0 8.5 39.0 2.0 40.0 58-59 26.302327743156205 33.0 8.0 38.0 2.0 40.0 60-61 26.010009103059836 33.0 8.0 38.0 2.0 40.0 62-63 25.83476640063364 33.0 7.0 38.0 2.0 40.0 64-65 25.608551650926533 32.0 4.0 37.5 2.0 40.0 66-67 25.345544038458364 32.0 2.0 37.0 2.0 40.0 68-69 25.104897776213235 32.0 2.0 36.5 2.0 39.5 70-71 24.68674170961514 31.5 2.0 36.0 2.0 39.0 72-73 24.47326808098698 31.0 2.0 36.0 2.0 39.0 74-75 24.449890955793784 32.0 2.0 35.5 2.0 39.0 76-77 24.221330449322632 31.0 2.0 35.0 2.0 38.0 78-79 23.94707175741308 31.0 2.0 35.0 2.0 37.0 80-81 23.56640874663792 31.0 2.0 35.0 2.0 37.0 82-83 23.677408598128785 31.0 2.0 35.0 2.0 36.0 84-85 23.534754987431725 31.0 2.0 35.0 2.0 36.0 86-87 23.426539132156627 31.0 2.0 35.0 2.0 36.0 88-89 23.267060536723008 31.0 2.0 35.0 2.0 35.0 90-91 23.133572964737333 31.0 2.0 35.0 2.0 35.0 92-93 23.01249484343287 31.0 2.0 35.0 2.0 35.0 94-95 22.90829959792527 31.0 2.0 35.0 2.0 35.0 96-97 22.790690127442836 31.0 2.0 35.0 2.0 35.0 98-99 22.704625096943463 31.0 2.0 34.0 2.0 35.0 100-101 22.59899701331632 31.0 2.0 34.0 2.0 35.0 102-103 21.5847331235871 29.0 2.0 33.5 2.0 34.5 104-105 22.333318024058478 30.5 2.0 34.0 2.0 35.0 106-107 22.475516894289 31.0 2.0 34.0 2.0 35.0 108-109 22.4366301902567 31.0 2.0 34.5 2.0 35.0 110-111 22.359822504084 31.0 2.0 34.5 2.0 35.0 112-113 22.254117140704153 31.0 2.0 34.0 2.0 35.0 114-115 22.14369908749388 31.0 2.0 34.0 2.0 35.0 116-117 22.029244886060493 31.0 2.0 34.0 2.0 35.0 118-119 21.893877023436943 31.0 2.0 34.0 2.0 35.0 120-121 21.705086850341296 31.0 2.0 34.0 2.0 35.0 122-123 21.484729548367227 31.0 2.0 34.0 2.0 35.0 124-125 20.60439147007541 28.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1319833095365937 1101 2 1.1809887661544174 1101 3 0.9964481899824982 1101 4 1.0600976120572376 1101 5 1.0342928369663724 1101 6 0.9682150584287825 1101 7 0.9485272419238768 1101 8 1.199492675501773 1101 9 1.6037441464606168 1101 10-11 1.5273709403474385 1101 12-13 1.4880297839409984 1101 14-15 1.7730099827277073 1101 16-17 1.3706738306484176 1101 18-19 1.3906063473672567 1101 20-21 1.5626396051335902 1101 22-23 1.3170190439461642 1101 24-25 1.2621099291973579 1101 26-27 1.5697360115731165 1101 28-29 1.491893407497706 1101 30-31 1.6083481820476635 1101 32-33 1.6379986948429774 1101 34-35 1.4801411564446738 1101 36-37 1.3171129880564578 1101 38-39 1.3255480387518368 1101 40-41 1.468304468211258 1101 42-43 1.6416351237912714 1101 44-45 1.467639675932702 1101 46-47 1.06219157741425 1101 48-49 0.9177663529813245 1101 50-51 0.9465759037395216 1101 52-53 0.9260314753093581 1101 54-55 0.8309542558393908 1101 56-57 0.8768031124958213 1101 58-59 0.781683762950415 1101 60-61 0.9458692927493644 1101 62-63 0.7693670834697741 1101 64-65 0.6940700914462674 1101 66-67 0.6258053595534605 1101 68-69 0.5366217615141196 1101 70-71 0.45418565784768106 1101 72-73 0.4106716452602015 1101 74-75 0.4052442726010632 1101 76-77 0.5128450035926555 1101 78-79 0.5403111800492333 1101 80-81 0.727286465468552 1101 82-83 0.7711198497351646 1101 84-85 0.753858760928722 1101 86-87 0.6550480036782176 1101 88-89 0.6237500135049565 1101 90-91 0.7368897378848906 1101 92-93 0.6732936933124769 1101 94-95 0.6502116462854985 1101 96-97 0.5629973119388616 1101 98-99 0.5957990223737006 1101 100-101 0.6200112080554163 1101 102-103 0.44557337192910396 1101 104-105 0.6282800772300305 1101 106-107 0.6351918336332503 1101 108-109 0.5779903397919206 1101 110-111 0.7298999915586393 1101 112-113 0.6548763807662361 1101 114-115 0.661761361857959 1101 116-117 0.5510227316778966 1101 118-119 0.47937276426017306 1101 120-121 0.5827426306168171 1101 122-123 0.5205652515805852 1101 124-125 0.45780849278592584 1102 1 0.22102946896720965 1102 2 0.6204303317873787 1102 3 0.4849367937341391 1102 4 0.25422413751832096 1102 5 0.28551444984149654 1102 6 0.10105991110905066 1102 7 0.1057477034571832 1102 8 0.06309832057224796 1102 9 0.7359476322477008 1102 10-11 0.7959358645318062 1102 12-13 0.9125740570367 1102 14-15 1.0145339671078943 1102 16-17 0.8133119154154933 1102 18-19 0.6079141821599165 1102 20-21 0.9705381540851157 1102 22-23 0.9101918158841862 1102 24-25 0.5664262512805394 1102 26-27 0.9897443659637766 1102 28-29 0.5116474392674206 1102 30-31 0.6860986761726195 1102 32-33 0.8669555378935954 1102 34-35 0.8200246912079905 1102 36-37 0.5891424243808068 1102 38-39 0.845619875270895 1102 40-41 0.851665361643736 1102 42-43 1.1203884703624993 1102 44-45 0.8308270702819414 1102 46-47 0.5693485499651167 1102 48-49 0.5469612917106517 1102 50-51 0.6293658624048533 1102 52-53 0.3845498934542526 1102 54-55 0.37290789263813195 1102 56-57 0.40738411310768186 1102 58-59 0.4859635735568091 1102 60-61 0.39002107535178965 1102 62-63 0.19435520713010845 1102 64-65 0.14651817671163414 1102 66-67 0.17313119374338015 1102 68-69 0.14251729578422356 1102 70-71 0.3286064656745573 1102 72-73 0.1075967956361481 1102 74-75 0.1859104518083292 1102 76-77 -0.013377580842789172 1102 78-79 0.04778184492480264 1102 80-81 0.17526809773512753 1102 82-83 0.1926546333424639 1102 84-85 0.13676946380858013 1102 86-87 0.2826362009646637 1102 88-89 0.21628712453898657 1102 90-91 0.3131128946327628 1102 92-93 0.3571609872883954 1102 94-95 0.268645346426549 1102 96-97 0.030603280382994313 1102 98-99 0.2728647086931666 1102 100-101 0.20891795629065513 1102 102-103 0.2102963147966328 1102 104-105 0.2251743193077722 1102 106-107 0.1673586708627326 1102 108-109 0.28983701174030685 1102 110-111 0.22613275355967133 1102 112-113 0.09333620782644303 1102 114-115 0.1184706729973577 1102 116-117 0.24256752835379558 1102 118-119 0.2626643667644508 1102 120-121 0.2482415229009689 1102 122-123 0.08505720298296637 1102 124-125 0.07889386720128755 1103 1 0.4885242372679066 1103 2 0.6571279243305241 1103 3 0.532841021262378 1103 4 0.2669703136480486 1103 5 0.5045799552704757 1103 6 0.5196224593177767 1103 7 0.45091775137981926 1103 8 0.6687702434268523 1103 9 1.125400495768428 1103 10-11 1.236475507280435 1103 12-13 1.1827110174119078 1103 14-15 1.245196048600718 1103 16-17 0.9236454533747711 1103 18-19 0.9987790588156642 1103 20-21 1.2109023887249393 1103 22-23 0.964205044766473 1103 24-25 0.9498898311289743 1103 26-27 1.1557238923050477 1103 28-29 1.1436871335851322 1103 30-31 1.1283783658264497 1103 32-33 1.2224536635195093 1103 34-35 0.972427409564709 1103 36-37 0.8757923181359395 1103 38-39 0.847475159379588 1103 40-41 1.0057324992220344 1103 42-43 1.1357590735576082 1103 44-45 0.8561692655015065 1103 46-47 0.7077146621519681 1103 48-49 0.6581294538575193 1103 50-51 0.6893058923365416 1103 52-53 0.4286660422345818 1103 54-55 0.5958377146805134 1103 56-57 0.6559417477833591 1103 58-59 0.6576728470728845 1103 60-61 0.698005716481525 1103 62-63 0.46050041005114295 1103 64-65 0.35142197125482255 1103 66-67 0.4160449599831928 1103 68-69 0.37271361674322634 1103 70-71 0.4925067188616765 1103 72-73 0.37538133058725975 1103 74-75 0.42891211910411897 1103 76-77 0.4141158157690761 1103 78-79 0.3879868604523615 1103 80-81 0.49602232854440587 1103 82-83 0.5439953458549667 1103 84-85 0.5375988020778522 1103 86-87 0.472736634807454 1103 88-89 0.49882373004636804 1103 90-91 0.5179360056997417 1103 92-93 0.5293664781301928 1103 94-95 0.4986405863106569 1103 96-97 0.5123656941146102 1103 98-99 0.45750197510072965 1103 100-101 0.4249383404095539 1103 102-103 0.3386551131693203 1103 104-105 0.36352053956585095 1103 106-107 0.4518969692586765 1103 108-109 0.5451392480673007 1103 110-111 0.5493243471085343 1103 112-113 0.5407788380045595 1103 114-115 0.5750582731116403 1103 116-117 0.5490293647557394 1103 118-119 0.5969135888871726 1103 120-121 0.572850969896983 1103 122-123 0.5001547375902149 1103 124-125 0.4509418334930295 1104 1 0.5369379803611771 1104 2 0.8542263977822735 1104 3 0.5071917757096251 1104 4 0.5934448691304901 1104 5 0.33144005070798244 1104 6 0.29068927500335207 1104 7 0.020747840376621696 1104 8 0.4441094795753493 1104 9 0.8251518165608047 1104 10-11 0.9428498379793417 1104 12-13 0.855817371687067 1104 14-15 0.9363504559788218 1104 16-17 0.6753896273114002 1104 18-19 0.7099321682827551 1104 20-21 0.9302529460769655 1104 22-23 0.7958081466991622 1104 24-25 0.8257842821285593 1104 26-27 0.9500880241236871 1104 28-29 0.9514433426102755 1104 30-31 0.9658687120620257 1104 32-33 0.8966513689512432 1104 34-35 0.8925978423104972 1104 36-37 0.7094427678353057 1104 38-39 0.7640582726006464 1104 40-41 0.7923333523695426 1104 42-43 1.054111514949934 1104 44-45 0.7943987934316645 1104 46-47 0.6341731616677784 1104 48-49 0.555512151502807 1104 50-51 0.5161898020325992 1104 52-53 0.455715698491705 1104 54-55 0.4118408319737412 1104 56-57 0.3134964583285118 1104 58-59 0.33459672537211915 1104 60-61 0.23345503828060998 1104 62-63 0.21588836908708942 1104 64-65 0.17443336702208967 1104 66-67 0.20992110304216283 1104 68-69 0.15502471454730227 1104 70-71 0.14919868834767414 1104 72-73 0.17884932778572704 1104 74-75 0.15476929502208492 1104 76-77 0.1197858278980668 1104 78-79 0.10759620280634508 1104 80-81 0.27271651798065477 1104 82-83 0.17972014977575768 1104 84-85 0.2506005357694434 1104 86-87 0.195432450940757 1104 88-89 0.18818747140615244 1104 90-91 0.19705223753229362 1104 92-93 0.18338180297873308 1104 94-95 0.18065637373929277 1104 96-97 0.13413887056150742 1104 98-99 0.16637933024439633 1104 100-101 0.24479597524388197 1104 102-103 0.25119611219714244 1104 104-105 0.25898203377555973 1104 106-107 0.3141380986000186 1104 108-109 0.268271099203897 1104 110-111 0.27792174020032334 1104 112-113 0.24505495273066558 1104 114-115 0.2969833824927015 1104 116-117 0.27504540416772727 1104 118-119 0.2858054720854959 1104 120-121 0.30131768987773455 1104 122-123 0.284811828211069 1104 124-125 0.06028011666409938 1105 1 0.7068694039415533 1105 2 1.0874039292672322 1105 3 0.71207907510491 1105 4 0.7702215413446467 1105 5 0.7361001390743844 1105 6 0.6412574009353129 1105 7 0.5533021644680716 1105 8 0.6554491787518799 1105 9 0.9223912517397608 1105 10-11 0.9217197040920446 1105 12-13 0.9884231379292139 1105 14-15 1.2626825421104897 1105 16-17 0.8125485648686954 1105 18-19 0.9661956189789436 1105 20-21 0.8892752894784053 1105 22-23 1.023601268598373 1105 24-25 0.6750678296894321 1105 26-27 1.0792949691091067 1105 28-29 1.164873481612279 1105 30-31 1.027845782436458 1105 32-33 1.069049360051654 1105 34-35 0.8575954423325314 1105 36-37 0.5310732577849073 1105 38-39 0.8026651640783662 1105 40-41 0.9153884849781591 1105 42-43 1.1244186727858647 1105 44-45 0.8708776323144534 1105 46-47 0.33461882970754786 1105 48-49 0.4500122675673168 1105 50-51 0.6342027186353363 1105 52-53 0.2645907973432564 1105 54-55 0.360897758664251 1105 56-57 0.4735788247854771 1105 58-59 0.44554987072029206 1105 60-61 0.3990373870328199 1105 62-63 0.3313423583619439 1105 64-65 0.1783344131764153 1105 66-67 0.03623607457685196 1105 68-69 -0.07268204748897844 1105 70-71 0.2869768005486115 1105 72-73 0.1894700171695618 1105 74-75 0.23951163181374824 1105 76-77 0.3188820001299284 1105 78-79 0.3116327886999848 1105 80-81 0.4785424644965559 1105 82-83 0.4570213745513989 1105 84-85 0.34500298059946033 1105 86-87 0.3616408595192979 1105 88-89 0.39653113251352323 1105 90-91 0.19713820395621084 1105 92-93 0.2964787974981249 1105 94-95 0.1272665032200706 1105 96-97 0.126690283204276 1105 98-99 0.23614590287914083 1105 100-101 0.314589301731381 1105 102-103 0.20868010906536583 1105 104-105 0.17842283890915311 1105 106-107 0.17383164895431236 1105 108-109 0.2104579906029258 1105 110-111 0.24386329876962165 1105 112-113 0.21799982405643803 1105 114-115 0.27634583424724113 1105 116-117 0.25188689545895215 1105 118-119 0.21099599492659848 1105 120-121 0.16036245930280302 1105 122-123 0.061258405094953616 1105 124-125 0.1088093432810382 1106 1 0.07983764153427231 1106 2 -1.5763204929943129 1106 3 -0.09977417705334446 1106 4 0.10062636651020185 1106 5 0.15207928945128302 1106 6 0.16782158694637772 1106 7 0.27692292136356755 1106 8 0.15667807569679937 1106 9 0.6748913109752834 1106 10-11 0.8827625022372381 1106 12-13 0.9337705718784015 1106 14-15 0.9227407658260844 1106 16-17 0.5762182983344601 1106 18-19 0.615013614154293 1106 20-21 0.9620653402160322 1106 22-23 0.6241798875846065 1106 24-25 0.7335713290679422 1106 26-27 0.7119767437157059 1106 28-29 0.905550434767548 1106 30-31 0.9418959680517673 1106 32-33 1.0724958588480504 1106 34-35 0.910297558737696 1106 36-37 0.5663263983882629 1106 38-39 0.9340316379254006 1106 40-41 0.9840092514794172 1106 42-43 1.2389742174548921 1106 44-45 0.9878529961006102 1106 46-47 0.8621724570668015 1106 48-49 0.6144530233997756 1106 50-51 0.6710622145378373 1106 52-53 0.6162019235033895 1106 54-55 0.6947855364868545 1106 56-57 0.62339149644842 1106 58-59 0.536995300715752 1106 60-61 0.6393717187248527 1106 62-63 0.4944078094516513 1106 64-65 0.4790805726333218 1106 66-67 0.43075246616655605 1106 68-69 0.29058043375577114 1106 70-71 0.3591514582473394 1106 72-73 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-1.3842017395554755 2216 24-25 -1.5396670347920463 2216 26-27 -1.3472218312893816 2216 28-29 -1.3298538570703258 2216 30-31 -1.1657258859086213 2216 32-33 -1.2693622632469186 2216 34-35 -1.6159638273942676 2216 36-37 -1.4622834650816117 2216 38-39 -1.4830842755474016 2216 40-41 -1.3785946658951573 2216 42-43 -1.1982096177208028 2216 44-45 -1.1547349893706738 2216 46-47 -1.5405241063285757 2216 48-49 -1.6045735969249932 2216 50-51 -4.731120403434204 2216 52-53 -4.473164619607658 2216 54-55 -3.433343110213592 2216 56-57 -1.9654050840819721 2216 58-59 -1.7432621359067397 2216 60-61 -1.6314735010114454 2216 62-63 -1.9213033118672982 2216 64-65 -1.8960250817829234 2216 66-67 -2.0445162887405637 2216 68-69 -2.0250339949924694 2216 70-71 -4.075265075378578 2216 72-73 -2.347430219974317 2216 74-75 -1.9813881613621405 2216 76-77 -1.852463385310088 2216 78-79 -1.8570644901010844 2216 80-81 -1.797850079006892 2216 82-83 -1.8718871680424556 2216 84-85 -1.8482468109521548 2216 86-87 -1.9102519059681455 2216 88-89 -1.8952587081994245 2216 90-91 -1.8303780658463857 2216 92-93 -1.8344565672594761 2216 94-95 -2.018121643917418 2216 96-97 -2.040236808588812 2216 98-99 -2.0005621286101025 2216 100-101 -2.068320096312995 2216 102-103 -1.735187718415169 2216 104-105 -1.7921978499992264 2216 106-107 -1.8369556903780015 2216 108-109 -2.012337600036929 2216 110-111 -1.8015448810698977 2216 112-113 -2.1503032977965333 2216 114-115 -2.035174322982396 2216 116-117 -1.7625591191161902 2216 118-119 -1.7481941424869092 2216 120-121 -1.915586386921845 2216 122-123 -1.8124532755946063 2216 124-125 -1.6568933172783638 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 508343.0 3 60278.0 4 34448.0 5 27651.0 6 27129.0 7 29515.0 8 32054.0 9 33487.0 10 34793.0 11 36020.0 12 37853.0 13 37988.0 14 36304.0 15 31410.0 16 26091.0 17 22696.0 18 20597.0 19 19464.0 20 19516.0 21 20817.0 22 25893.0 23 35543.0 24 49589.0 25 62046.0 26 69758.0 27 77815.0 28 89162.0 29 100900.0 30 115053.0 31 131621.0 32 156100.0 33 190996.0 34 240051.0 35 312894.0 36 413245.0 37 385510.0 38 83413.0 39 97.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0843726591399 18.440892783026694 14.250673974789402 23.224060583044 2 13.59327776132711 19.99905707352014 42.16753432445383 24.24013084069891 3 19.589518889347417 25.017185675918423 27.768714456270594 27.624580978463563 4 11.835564449065883 16.57122998443023 37.68599258332724 33.90721298317665 5 13.63069246886514 38.68663285503728 33.589615257060906 14.093059419036672 6 32.08520151558798 37.016485947456125 16.962897314641634 13.935415222314262 7 28.522597347040257 32.819682090261495 20.10023296916053 18.557487593537726 8 25.253626473442147 36.439344188203755 19.648311237499872 18.658718100854227 9 25.337367858284555 17.800577620512144 19.76054653482809 37.10150798637521 10-11 24.240154470674767 27.81393450310083 27.04422523181687 20.90168579440753 12-13 25.27348040080659 24.91338094161697 27.304181283396016 22.50895737418042 14-15 22.066683557369355 26.363865689430675 25.33508963576288 26.23436111743709 16-17 22.111241085709825 29.597047983490697 25.600790480625495 22.69092045017398 18-19 22.14008256521143 28.463698791968874 27.516658044597335 21.879560598222362 20-21 22.895280347335227 27.183532986431597 27.757846755249126 22.163339910984053 22-23 22.55698586504175 26.99775371810499 27.569575901647518 22.875684515205737 24-25 22.607570411934912 26.98350653840118 27.660596968820606 22.748326080843302 26-27 22.29710877733163 27.018077172056255 27.897660289883202 22.78715376072891 28-29 22.2726635941755 27.5465312702451 27.613368077820528 22.567437057758877 30-31 22.343624090455794 27.08518772491133 27.536935073076858 23.03425311155602 32-33 22.36398729845907 26.941160374796585 27.62765642461295 23.067195902131395 34-35 22.929331908738916 26.89237343434402 27.5205226157118 22.657772041205266 36-37 22.796638367518725 26.720342498467154 27.489823710135635 22.993195423878486 38-39 22.370503687810515 27.067249346744287 27.68141372981148 22.880833235633713 40-41 22.773874944314407 26.964352814552818 27.611745640195206 22.65002660093757 42-43 22.25952481116254 26.805109094215833 27.62068727308377 23.31467882153786 44-45 22.6023874330549 27.001121261543208 27.37551792916492 23.02097337623697 46-47 23.01220193272444 27.3618166755845 26.78560779315042 22.840373598540644 48-49 22.86447920287847 27.256848128316907 26.88877552088527 22.989897147919354 50-51 22.680660726642838 27.388800858607453 27.097015369425993 22.83352304532372 52-53 23.056349059287644 27.36598473947093 26.62625480550988 22.951411395731544 54-55 22.81084430238563 27.35240869630259 26.707248383552184 23.129498617759594 56-57 22.828600091767093 27.45621261975213 26.718177639436874 22.997009649043903 58-59 23.117184687668438 27.099835214154123 26.62753909429204 23.1554410038854 60-61 22.807184511724504 27.334753517048192 27.015398117198348 22.84266385402895 62-63 22.745729155878923 27.38264956492807 27.068753814729206 22.802867464463798 64-65 22.70090950215963 27.522255570864257 26.7447341622066 23.03210076476951 66-67 22.752260506887232 27.470351350149347 26.526934210296226 23.250453932667195 68-69 22.86551953426588 27.248637870350624 26.80526843892348 23.080574156460017 70-71 22.995682342936206 27.03634620129899 26.791698245397583 23.176273210367224 72-73 22.95212388475349 27.193891170084928 26.653870187088973 23.20011475807261 74-75 22.67457572098557 27.756149545334463 26.527045829719004 23.042228903960964 76-77 23.00186608165264 27.22391713011183 26.585742541319 23.18847424691653 78-79 23.025975149080182 27.27489801516525 26.700031837555755 22.99909499819881 80-81 23.05197881536602 27.316738813559134 26.684370107498683 22.94691226357616 82-83 22.9825974155112 27.30193213461686 26.846963055574264 22.868507394297673 84-85 22.823452005477346 27.500429638916028 26.721130743528704 22.95498761207792 86-87 22.87336718274578 27.537004831130808 26.640985300546916 22.948642685576498 88-89 22.91004768490417 27.499029319308704 26.421095126156814 23.169827869630318 90-91 22.864390328279477 27.360901398271263 26.615396353135797 23.159311920313467 92-93 22.960125983904963 27.451201550147392 26.683592138813662 22.905080327133984 94-95 22.896288027346795 27.29250910926211 26.768998590013503 23.042204273377592 96-97 22.880842711508738 27.19933702110979 26.92271935385727 22.9971009135242 98-99 22.74033461751987 27.428315576320085 26.554730847622338 23.27661895853771 100-101 22.575124859842063 27.246082979005536 26.90225454787801 23.276537613274392 102-103 22.513560170017314 26.904774105530194 27.165949261491427 23.415716462961065 104-105 22.71208210115644 27.101392581919885 26.884457848362693 23.302067468560985 106-107 22.59894583180541 27.21677371434347 26.933891340836013 23.25038911301511 108-109 22.47494841774479 27.20277782597906 26.84126822800252 23.48100552827363 110-111 22.41218908770882 27.38104433403125 26.86970063834319 23.337065939916737 112-113 22.63049896369027 27.299957115844354 26.842425164395 23.227118756070382 114-115 22.46249560156543 27.476793611408013 26.751306589749817 23.30940419727674 116-117 22.367007900976013 27.389068102258225 26.956028147734717 23.28789584903104 118-119 22.404129078863907 27.296040286489536 26.96278807526957 23.337042559376982 120-121 22.25515464981745 27.646639915148302 26.915556539215697 23.182648895818556 122-123 22.226020617069207 27.44328956719685 26.851533916667652 23.479155899066292 124-125 22.351655801092953 27.366101777985445 26.896239255004467 23.38600316591713 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5051.0 1 3509.5 2 1536.5 3 1016.5 4 882.5 5 825.5 6 873.0 7 1038.5 8 1249.5 9 1399.0 10 1500.0 11 1580.0 12 1667.0 13 1797.5 14 1992.5 15 2209.0 16 2445.5 17 2734.0 18 3071.5 19 3529.5 20 4295.5 21 5197.5 22 6294.5 23 8061.0 24 10345.5 25 13206.0 26 17192.0 27 21967.5 28 26646.0 29 32007.5 30 38758.0 31 46659.5 32 55848.5 33 63192.0 34 69327.5 35 76371.0 36 83603.0 37 90510.0 38 96968.5 39 103097.5 40 108231.0 41 113158.5 42 117081.0 43 120720.5 44 125233.0 45 129136.0 46 131968.5 47 137823.5 48 144703.0 49 143776.5 50 136485.0 51 130991.0 52 128757.0 53 127207.5 54 124059.0 55 115997.5 56 106729.0 57 98688.5 58 90297.5 59 80319.5 60 67823.0 61 56683.5 62 46483.5 63 35485.5 64 26298.0 65 20027.0 66 15144.0 67 11503.0 68 9163.0 69 7416.5 70 5822.5 71 4198.0 72 3131.0 73 2431.0 74 1755.5 75 1237.5 76 916.0 77 647.5 78 441.0 79 321.5 80 224.0 81 171.0 82 127.0 83 87.5 84 73.5 85 57.0 86 40.0 87 27.5 88 27.5 89 22.0 90 12.5 91 10.0 92 10.5 93 7.0 94 2.0 95 3.5 96 3.5 97 2.0 98 2.0 99 1.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21082796591990408 2 0.2512004488276029 3 0.3032886522521135 4 0.2894553015010423 5 0.32303486664429865 6 0.2339018849659254 7 0.22529385557211767 8 0.20563014625399464 9 0.2051626175009763 10-11 0.149017914601748 12-13 0.17352192159817828 14-15 0.1904354617808995 16-17 0.1634563025626076 18-19 0.18941789920080085 20-21 0.18365629486213403 22-23 0.20563014625399464 24-25 0.19681585417503175 26-27 0.2481752627786609 28-29 0.17610708058545602 30-31 0.1562508594278548 32-33 0.1961283118911813 34-35 0.1549170273971849 36-37 0.1551645426193711 38-39 0.1875202824973736 40-41 0.20649644953164617 42-43 0.27335306121326464 44-45 0.1732606555303151 46-47 0.15875351334107046 48-49 0.1616549417789194 50-51 0.16710027666701502 52-53 0.18818032308987004 54-55 0.17448448079556894 56-57 0.1732194029932841 58-59 0.14760157749701608 60-61 0.16111865879751605 62-63 0.15059926185460407 64-65 0.14596522686145197 66-67 0.13254440148069105 68-69 0.13666965518379381 70-71 0.1376184635355074 72-73 0.11417327165620685 74-75 0.11508082747088946 76-77 0.1375634601527994 78-79 0.14322880857172718 80-81 0.15356944452083804 82-83 0.15603084589702268 84-85 0.1425687679792307 86-87 0.1688878866050262 88-89 0.16378632285885583 90-91 0.15164432612605674 92-93 0.15174058204579582 94-95 0.17375568597468746 96-97 0.16111865879751605 98-99 0.15666338479816508 100-101 0.13664215349243977 102-103 0.1757908111348848 104-105 0.13375447590026787 106-107 0.14095991903502067 108-109 0.15211185487907505 110-111 0.1273190801234276 112-113 0.11722595939650288 114-115 0.11457204618084012 116-117 0.12027864713679891 118-119 0.10329635272569263 120-121 0.09485333348000902 122-123 0.12345509248818803 124-125 0.11916482863696116 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3636140.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.29615003743659 #Duplication Level Percentage of deduplicated Percentage of total 1 83.02645206288052 53.38281218911003 2 9.162770787599632 11.782617706362938 3 3.236343023726106 6.242531898783148 4 1.5143549256878683 3.8946876604783327 5 0.836813807740882 2.6901953067953177 6 0.5142294108466786 1.9837782812076403 7 0.3349733981567788 1.5076249906506762 8 0.23520413703681364 1.2098176387475812 9 0.17793799074821856 1.0296654975456716 >10 0.8825178855214503 10.070753355265415 >50 0.0543169610093255 2.400041278236175 >100 0.022101547908684065 2.455611697155869 >500 0.0012786171872051252 0.5485685679607017 >1k 7.054439497057693E-4 0.8012939317005028 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3665 0.10079369881247696 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 2.750169135401827E-5 0.0 7 0.0 0.0 0.0 2.750169135401827E-5 0.0 8 0.0 0.0 0.0 2.750169135401827E-5 0.0 9 0.0 0.0 0.0 5.500338270803654E-5 0.0 10-11 2.750169135401827E-5 0.0 0.0 8.250507406205481E-5 2.750169135401827E-5 12-13 5.500338270803654E-5 0.0 0.0 8.250507406205481E-5 1.925118394781279E-4 14-15 8.250507406205481E-5 0.0 0.0 8.250507406205481E-5 2.2001353083214617E-4 16-17 9.625591973906394E-5 0.0 0.0 1.1000676541607308E-4 2.4751522218616445E-4 18-19 1.2375761109308223E-4 0.0 0.0 1.1000676541607308E-4 2.4751522218616445E-4 20-21 1.3750845677009137E-4 0.0 0.0 1.3750845677009137E-4 2.4751522218616445E-4 22-23 1.6501014812410962E-4 0.0 0.0 2.337643765091553E-4 2.4751522218616445E-4 24-25 1.925118394781279E-4 0.0 0.0 2.4751522218616445E-4 2.4751522218616445E-4 26-27 1.925118394781279E-4 0.0 0.0 3.437711419252284E-4 2.7501691354018273E-4 28-29 2.2001353083214617E-4 0.0 0.0 7.150439752044751E-4 2.7501691354018273E-4 30-31 2.4751522218616445E-4 0.0 0.0 0.002048876005874361 2.7501691354018273E-4 32-33 2.612660678631736E-4 0.0 0.0 0.004950304443723289 3.02518604894201E-4 34-35 2.7501691354018273E-4 0.0 0.0 0.008360514171621555 3.02518604894201E-4 36-37 3.3002029624821925E-4 0.0 0.0 0.01432838119544352 3.02518604894201E-4 38-39 3.5752198760223753E-4 0.0 0.0 0.024682767990231398 3.162694505712101E-4 40-41 3.712728332792467E-4 0.0 0.0 0.037031027408185604 3.3002029624821925E-4 42-43 3.9877452463326496E-4 0.0 0.0 0.049544296974263914 3.3002029624821925E-4 44-45 4.4002706166429233E-4 1.3750845677009135E-5 0.0 0.06374892055861435 3.3002029624821925E-4 46-47 4.950304443723289E-4 2.750169135401827E-5 0.0 0.0815700165560182 3.3002029624821925E-4 48-49 5.775355184343838E-4 2.750169135401827E-5 0.0 0.10058743612732182 3.3002029624821925E-4 50-51 5.775355184343838E-4 2.750169135401827E-5 0.0 0.1229738128894927 3.3002029624821925E-4 52-53 6.187880554654111E-4 2.750169135401827E-5 0.0 0.14633649969473123 3.712728332792467E-4 54-55 6.600405924964385E-4 2.750169135401827E-5 0.0 0.1716930591231361 3.850236789562558E-4 56-57 6.875422838504568E-4 2.750169135401827E-5 0.0 0.19986854191532777 4.1252537031027405E-4 58-59 7.150439752044751E-4 2.750169135401827E-5 0.0 0.2335856155153542 4.4002706166429233E-4 60-61 7.150439752044751E-4 2.750169135401827E-5 0.0 0.26999785486807437 4.4002706166429233E-4 62-63 7.425456665584934E-4 2.750169135401827E-5 0.0 0.30839021599828387 4.4002706166429233E-4 64-65 7.425456665584934E-4 2.750169135401827E-5 0.0 0.34906521751087694 4.4002706166429233E-4 66-67 7.700473579125116E-4 2.750169135401827E-5 0.0 0.3982107399605076 4.4002706166429233E-4 68-69 7.700473579125116E-4 2.750169135401827E-5 0.0 0.45551051389660463 4.4002706166429233E-4 70-71 7.837982035895207E-4 2.750169135401827E-5 0.0 0.5196307072884983 4.537779073413015E-4 72-73 7.975490492665299E-4 2.750169135401827E-5 0.0 0.5882061746797428 4.8127959869531976E-4 74-75 8.112998949435391E-4 2.750169135401827E-5 0.0 0.6583492384781664 5.362829814033563E-4 76-77 8.250507406205482E-4 2.750169135401827E-5 0.0 0.7357389979483738 6.05037209788402E-4 78-79 8.388015862975573E-4 2.750169135401827E-5 0.0 0.829217246860682 6.05037209788402E-4 80-81 0.0010450642714526944 2.750169135401827E-5 0.0 0.934699984049019 6.05037209788402E-4 82-83 0.001100067654160731 2.750169135401827E-5 0.0 1.0529572568712977 6.05037209788402E-4 84-85 0.0011138184998377401 2.750169135401827E-5 0.0 1.1704032297986326 6.05037209788402E-4 86-87 0.0011275693455147491 2.750169135401827E-5 0.0 1.292992019009169 6.462897468194294E-4 88-89 0.0011275693455147491 2.750169135401827E-5 0.0 1.4371283833955788 6.875422838504568E-4 90-91 0.0012375761109308223 2.750169135401827E-5 0.0 1.599663379297827 6.875422838504568E-4 92-93 0.001320081184992877 2.750169135401827E-5 0.0 1.7796894509012304 6.875422838504568E-4 94-95 0.001320081184992877 2.750169135401827E-5 0.0 1.9603205597144227 7.01293129527466E-4 96-97 0.001320081184992877 2.750169135401827E-5 0.0 2.1456407069034746 7.425456665584934E-4 98-99 0.0014163371047319412 2.750169135401827E-5 0.0 2.354557305274274 7.700473579125116E-4 100-101 0.001512593024471005 4.1252537031027406E-5 0.0 2.6003124192137816 7.700473579125116E-4 102-103 0.0015675964071790414 5.500338270803654E-5 0.0 2.870571540149719 7.700473579125116E-4 104-105 0.0018701150120732425 5.500338270803654E-5 0.0 3.1386992800057207 7.700473579125116E-4 106-107 0.001993872623166325 5.500338270803654E-5 0.0 3.403829335504134 7.700473579125116E-4 108-109 0.002048876005874361 5.500338270803654E-5 0.0 3.6957185366900065 7.700473579125116E-4 110-111 0.002131381079936416 6.875422838504568E-5 0.0 4.02388246877183 7.700473579125116E-4 112-113 0.002145131925613425 1.1000676541607308E-4 0.0 4.3843608881946246 7.700473579125116E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 12115 0.0 30.8309 2 GTACATG 12725 0.0 30.430119 1 ACATGGG 12925 0.0 28.115211 3 GTATCAA 16750 0.0 22.12196 1 CATGGGG 7470 0.0 21.929876 4 GAGTACA 9395 0.0 21.051805 1 AGAGTAC 11065 0.0 20.888477 10-11 GTGTAGC 2245 0.0 19.901829 1 CATGGGA 6200 0.0 19.696373 4 TCAACGC 18730 0.0 19.464252 4 CAACGCA 18655 0.0 19.416653 5 ATCAACG 18775 0.0 19.323215 3 TATCAAC 19065 0.0 19.310509 2 AACGCAG 18830 0.0 19.028252 6 AGGACCG 1210 0.0 18.709589 5 AGTACAT 9645 0.0 18.591154 2 TGTACCG 770 0.0 17.79521 5 GGACCGA 950 0.0 17.542326 6 GAGTACT 6125 0.0 17.531792 12-13 ACGCAGA 20395 0.0 17.184961 7 >>END_MODULE