FastQCFastQC Report
Thu 26 May 2016
SRR1364621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3636140
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79020.21731836507945237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66140.18189618661547685No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52370.1440263576209937No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45910.1262602650062979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG133550.027.0650232
GTACATG140000.027.0224441
ACATGGG136900.025.121263
GTATCAA217600.024.0115171
GGTATCA174600.022.9641171
GGACCGA11750.021.7927576
AGAGTAC183000.021.6993110-11
GAGTACA107400.021.3012891
GAGTACT126200.021.02093112-13
TCAACGC250650.020.7498284
ATCAACG252000.020.6833363
CAACGCA250300.020.53885
TATCAAC252250.020.3960572
GTACTTT129800.020.32322714-15
AACGCAG255300.020.1765486
AGTACTT133850.020.17541912-13
AGTACAT106750.019.858472
ACCGAGT14050.019.4970578
CATGGGG82750.019.4163044
AGGACCG14550.019.2424345
Produced by FastQC (version 0.11.5)