##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364621_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3636140 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.47354612308657 31.0 30.0 33.0 26.0 34.0 2 29.589316142942792 31.0 30.0 34.0 26.0 34.0 3 29.698841078726343 31.0 30.0 34.0 26.0 34.0 4 33.39803417910201 35.0 33.0 37.0 28.0 37.0 5 32.738399786586875 35.0 33.0 37.0 26.0 37.0 6 32.76620592166418 35.0 33.0 37.0 26.0 37.0 7 32.560902770520386 35.0 33.0 37.0 25.0 37.0 8 32.4991895251558 35.0 33.0 37.0 24.0 37.0 9 33.79364243400969 38.0 33.0 39.0 23.0 39.0 10-11 33.591448349073474 37.0 33.0 39.0 22.0 39.0 12-13 33.44871443343766 37.0 33.0 39.0 20.0 39.0 14-15 34.439617698988485 38.0 33.0 41.0 20.0 41.0 16-17 34.3486586050042 38.0 33.0 41.0 19.0 41.0 18-19 34.37736239528731 38.5 33.0 41.0 19.0 41.0 20-21 34.38055369155203 39.0 33.0 41.0 19.0 41.0 22-23 34.377417948703844 39.0 33.0 41.0 19.0 41.0 24-25 34.36477377108692 39.0 33.0 41.0 19.0 41.0 26-27 34.266421122399024 39.0 33.0 41.0 19.0 41.0 28-29 34.044877122443026 38.0 33.0 41.0 18.0 41.0 30-31 33.99153346680821 38.0 33.0 41.0 18.0 41.0 32-33 33.82939999559973 38.0 33.0 40.0 17.0 41.0 34-35 33.692891225310355 38.0 33.0 40.0 16.0 41.0 36-37 33.52923828565457 38.0 32.5 40.0 15.5 41.0 38-39 33.399248791300664 38.0 32.0 40.0 15.0 41.0 40-41 33.235897957724404 38.0 32.0 40.0 11.0 41.0 42-43 33.059685545661054 38.0 32.0 40.0 9.0 41.0 44-45 32.86950626213512 38.0 32.0 40.0 8.0 41.0 46-47 32.64538989147833 38.0 31.0 40.0 4.5 41.0 48-49 32.40214018162117 38.0 31.0 40.0 2.0 41.0 50-51 32.2042734878195 38.0 31.0 40.0 2.0 41.0 52-53 32.0366321153751 37.5 30.5 40.0 2.0 41.0 54-55 31.8168095837894 37.0 30.0 40.0 2.0 41.0 56-57 31.576863652114604 37.0 30.0 40.0 2.0 41.0 58-59 31.378276689016374 36.5 30.0 40.0 2.0 41.0 60-61 31.150004812795984 36.0 29.0 40.0 2.0 41.0 62-63 30.883947400265114 36.0 29.0 39.5 2.0 41.0 64-65 30.605332165428173 35.0 29.0 39.0 2.0 41.0 66-67 30.31710316434461 35.0 29.0 39.0 2.0 40.5 68-69 29.985602452050802 35.0 28.5 38.5 2.0 40.0 70-71 29.721444718850208 35.0 28.5 38.0 2.0 40.0 72-73 29.568544803005384 35.0 29.0 37.0 2.0 40.0 74-75 29.29355181593668 34.5 28.5 37.0 2.0 39.0 76-77 29.007043458172678 34.0 28.0 36.0 2.0 39.0 78-79 28.710002365145456 34.0 28.0 36.0 2.0 38.5 80-81 28.441986832190178 34.0 28.0 35.5 2.0 37.0 82-83 28.189576858976828 34.0 27.0 35.0 2.0 37.0 84-85 28.18857469734389 34.0 28.0 35.0 2.0 36.5 86-87 28.060002365145458 34.0 28.0 35.0 2.0 36.0 88-89 27.92110273531822 34.0 28.0 35.0 2.0 36.0 90-91 27.765933654919777 34.0 27.0 35.0 2.0 36.0 92-93 27.634982151402312 34.0 27.0 35.0 2.0 35.0 94-95 27.55258749663104 34.0 27.0 35.0 2.0 35.0 96-97 27.46767904976156 34.0 27.0 35.0 2.0 35.0 98-99 27.381994092636695 34.0 27.0 35.0 2.0 35.0 100-101 27.28844461434378 34.0 26.5 35.0 2.0 35.0 102-103 26.09525472066532 32.0 24.5 34.0 2.0 35.0 104-105 27.032887347571876 33.5 25.5 35.0 2.0 35.0 106-107 27.19991185707921 34.0 27.0 35.0 2.0 35.0 108-109 27.219744564290703 34.0 27.0 35.0 2.0 35.0 110-111 27.16237273592326 34.0 27.0 35.0 2.0 35.0 112-113 27.11353880763667 34.0 27.0 35.0 2.0 35.0 114-115 27.035178788495493 34.0 26.0 35.0 2.0 35.0 116-117 26.918827795409417 34.0 25.5 35.0 2.0 35.0 118-119 26.814256464272553 34.0 25.0 35.0 2.0 35.0 120-121 26.680701650651514 34.0 25.0 35.0 2.0 35.0 122-123 26.50470980215283 34.0 25.0 35.0 2.0 35.0 124-125 25.695788390986046 33.0 22.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7836983990434447 1101 2 0.7815730103143217 1101 3 0.6802020082525289 1101 4 0.8081072451956501 1101 5 0.9980177996846749 1101 6 1.061033215187635 1101 7 0.9829898813126547 1101 8 0.97395640539429 1101 9 1.1333878625736489 1101 10-11 1.1784462615692348 1101 12-13 1.2791128871939605 1101 14-15 1.278648280647161 1101 16-17 1.3643641813106058 1101 18-19 1.345009010091374 1101 20-21 1.3842252483233182 1101 22-23 1.2750916341311296 1101 24-25 1.1988354869154705 1101 26-27 1.2480005571103518 1101 28-29 1.338560422981793 1101 30-31 1.2724429724961013 1101 32-33 1.3588815521153208 1101 34-35 1.298836111633868 1101 36-37 1.2503423618804916 1101 38-39 1.2683648176807552 1101 40-41 1.2780050826685994 1101 42-43 1.255322387773198 1101 44-45 1.1426660819578203 1101 46-47 1.2081304877977246 1101 48-49 1.2180636733213035 1101 50-51 1.13565313218524 1101 52-53 1.1503075329168624 1101 54-55 1.1791855531720365 1101 56-57 1.1757047616098042 1101 58-59 1.227864899910589 1101 60-61 1.2177834355840709 1101 62-63 1.1634769051541518 1101 64-65 1.1266862661601031 1101 66-67 1.0061306451482643 1101 68-69 1.0435181613813356 1101 70-71 1.0344080202337267 1101 72-73 1.1117631331328042 1101 74-75 1.0305086925578912 1101 76-77 0.9904056878281153 1101 78-79 0.9533367889068209 1101 80-81 1.0280393784788728 1101 82-83 1.0257333125575983 1101 84-85 1.1156983617249914 1101 86-87 1.0508639022782553 1101 88-89 0.9956655987170144 1101 90-91 1.064983219780153 1101 92-93 1.0561727020291478 1101 94-95 0.9255524360532945 1101 96-97 0.9767146377147533 1101 98-99 1.0911187818553465 1101 100-101 1.045134833856423 1101 102-103 1.013582678373833 1101 104-105 1.0277889432503677 1101 106-107 1.0335280182576483 1101 108-109 0.9710880723260757 1101 110-111 1.0159969187160485 1101 112-113 1.0490143886779641 1101 114-115 1.0187654559978476 1101 116-117 1.012869978430711 1101 118-119 1.0974209999653475 1101 120-121 1.087015903069755 1101 122-123 1.0496498430751124 1101 124-125 0.9314727529477942 1102 1 0.39874121658071715 1102 2 -0.5183690456224674 1102 3 0.07337187407713586 1102 4 0.3044949256599807 1102 5 0.594888258239898 1102 6 0.6184756996733398 1102 7 0.6311747290429395 1102 8 0.5864749824158579 1102 9 0.8575562669110823 1102 10-11 0.9853806559903688 1102 12-13 1.0258945893443467 1102 14-15 1.0755783453705519 1102 16-17 1.1620578033443678 1102 18-19 1.141052915844746 1102 20-21 1.0809284648494 1102 22-23 1.1002185726852787 1102 24-25 1.0907775369577877 1102 26-27 1.1852807008916884 1102 28-29 1.232845972476781 1102 30-31 1.166467023189135 1102 32-33 1.0562157516623643 1102 34-35 0.8966878975193779 1102 36-37 0.8719700622955671 1102 38-39 0.9092702454164439 1102 40-41 0.8099161784906244 1102 42-43 0.8234870599481781 1102 44-45 0.6836708967160448 1102 46-47 0.7348232937208294 1102 48-49 0.7542509710971075 1102 50-51 0.8050784304371206 1102 52-53 0.7240864872136399 1102 54-55 0.7019096979642008 1102 56-57 0.555815348820861 1102 58-59 0.7623940166652723 1102 60-61 0.6393273522010894 1102 62-63 0.7636563747925642 1102 64-65 0.7194094130038913 1102 66-67 0.7591392436113296 1102 68-69 0.5365791644716182 1102 70-71 0.45466817495821843 1102 72-73 0.511922493014719 1102 74-75 0.6843669727843995 1102 76-77 0.7336825589419149 1102 78-79 0.6376234131154455 1102 80-81 0.5689303133447154 1102 82-83 0.5132560448415653 1102 84-85 0.5719655529451302 1102 86-87 0.503750183524371 1102 88-89 0.6631723994679177 1102 90-91 0.5570265691224705 1102 92-93 0.6871650444888466 1102 94-95 0.4816862442071752 1102 96-97 0.5065664197620059 1102 98-99 0.5096310018909023 1102 100-101 0.3560836744028464 1102 102-103 0.3252027694325079 1102 104-105 0.3328753339829369 1102 106-107 0.5488417623303583 1102 108-109 0.6132252055123573 1102 110-111 0.5065543976297633 1102 112-113 0.5477049241314162 1102 114-115 0.6995454157139065 1102 116-117 0.6681363528547735 1102 118-119 0.6201021527848987 1102 120-121 0.6497681617675859 1102 122-123 0.650512352176424 1102 124-125 0.5437402321747378 1103 1 0.2311971665059147 1103 2 -0.983783170474883 1103 3 0.024429151883591516 1103 4 0.31300371998455034 1103 5 0.5096384598962871 1103 6 0.6640623867463518 1103 7 0.5167844231103018 1103 8 0.6166897936287299 1103 9 0.6905828589156613 1103 10-11 0.8070833709230953 1103 12-13 0.8028711654039995 1103 14-15 0.8395210358346361 1103 16-17 0.8756314279229684 1103 18-19 0.8897590862199323 1103 20-21 0.9800511593120973 1103 22-23 0.9554957253075216 1103 24-25 0.8016909255124602 1103 26-27 0.7916845630430274 1103 28-29 0.9270585439386636 1103 30-31 0.9385606450313304 1103 32-33 0.9353139509843018 1103 34-35 1.0522896076185617 1103 36-37 1.0571363040780781 1103 38-39 1.0357863119930713 1103 40-41 1.132127401555195 1103 42-43 1.0762490045719773 1103 44-45 1.0681524168178456 1103 46-47 1.0664368614318747 1103 48-49 0.9909450664264838 1103 50-51 1.0220770755544493 1103 52-53 0.956176864980371 1103 54-55 1.0163765625436447 1103 56-57 1.0326777406765864 1103 58-59 0.9591984489147514 1103 60-61 1.004620779594017 1103 62-63 0.9244802827919258 1103 64-65 0.9740448948910085 1103 66-67 0.9788280759652217 1103 68-69 0.8593705658864152 1103 70-71 0.9844662145240832 1103 72-73 0.9223185240571468 1103 74-75 0.8371186454323585 1103 76-77 0.743166127511401 1103 78-79 0.8067342010122545 1103 80-81 0.9032535243769146 1103 82-83 0.8723339505607264 1103 84-85 0.8609020596518668 1103 86-87 0.8674338465569811 1103 88-89 0.8339935096962598 1103 90-91 0.8565599996618403 1103 92-93 0.8323904202161714 1103 94-95 0.7732372407317882 1103 96-97 0.7277952814060242 1103 98-99 0.7841922166256161 1103 100-101 0.8191930726191785 1103 102-103 0.7788151576445799 1103 104-105 0.8094670840312475 1103 106-107 0.7262556260946731 1103 108-109 0.7881577734428618 1103 110-111 0.8501565396679673 1103 112-113 0.7900439164889406 1103 114-115 0.7648355376645029 1103 116-117 0.7683138843964699 1103 118-119 0.806509295273937 1103 120-121 0.7537621367969933 1103 122-123 0.806278654475026 1103 124-125 0.7160665390256433 1104 1 0.133212178444591 1104 2 -0.8588051952716995 1104 3 -0.023111401729394743 1104 4 0.264281273539666 1104 5 0.46594248425495266 1104 6 0.37560620949390966 1104 7 0.38887390176054737 1104 8 0.5369113150830316 1104 9 0.5222735172605013 1104 10-11 0.6813277855465714 1104 12-13 0.6185442584602896 1104 14-15 0.6742612422886509 1104 16-17 0.8017596657377197 1104 18-19 0.729525441204764 1104 20-21 0.8116013915553921 1104 22-23 0.6969924066786461 1104 24-25 0.624862117668485 1104 26-27 0.48624479951386235 1104 28-29 0.7716909118311008 1104 30-31 0.6246810336393196 1104 32-33 0.7252083975059946 1104 34-35 0.7047746967378288 1104 36-37 0.7078647523945918 1104 38-39 0.658027581099013 1104 40-41 0.7868594609546022 1104 42-43 0.7590999024136309 1104 44-45 0.6790283988761416 1104 46-47 0.7572431294532791 1104 48-49 0.7455247993797585 1104 50-51 0.6928513456253711 1104 52-53 0.4957420166191895 1104 54-55 0.6674960633750722 1104 56-57 0.6755943319483997 1104 58-59 0.6353387973994522 1104 60-61 0.5924266647554219 1104 62-63 0.5219704465193651 1104 64-65 0.5398737983312465 1104 66-67 0.5426560558735858 1104 68-69 0.519873410401221 1104 70-71 0.6182715959901728 1104 72-73 0.5658396480847188 1104 74-75 0.6150399476530062 1104 76-77 0.5913077401193618 1104 78-79 0.5898252497073919 1104 80-81 0.5869599545456055 1104 82-83 0.5716059839571344 1104 84-85 0.58632637240866 1104 86-87 0.5849459567180766 1104 88-89 0.5768466539484507 1104 90-91 0.5428316698738485 1104 92-93 0.5312098470062914 1104 94-95 0.4838340905056775 1104 96-97 0.4601190801479902 1104 98-99 0.49164436302644887 1104 100-101 0.4959281669456921 1104 102-103 0.49388328711734175 1104 104-105 0.4736104909180696 1104 106-107 0.5365972426513217 1104 108-109 0.47299073338083275 1104 110-111 0.39238281596804825 1104 112-113 0.3780568194748781 1104 114-115 0.43814945253931725 1104 116-117 0.44452152003478673 1104 118-119 0.33506488111182264 1104 120-121 0.3658060861129222 1104 122-123 0.37497522547282713 1104 124-125 0.4502914076384421 1105 1 0.43905651823425984 1105 2 0.1761845162981608 1105 3 0.4282215696400513 1105 4 0.514971955723567 1105 5 0.611024299897295 1105 6 0.5552437734769242 1105 7 0.6702294171853254 1105 8 0.6274009105883849 1105 9 0.6740658286753458 1105 10-11 0.7254057415522226 1105 12-13 0.772858710655477 1105 14-15 0.8384016370653598 1105 16-17 0.8949091056772858 1105 18-19 0.8593261840176254 1105 20-21 0.9111024125296296 1105 22-23 0.7822114931919515 1105 24-25 0.8423580554106991 1105 26-27 0.89646359094791 1105 28-29 1.0281859013852923 1105 30-31 0.9374754735592177 1105 32-33 0.88083191965071 1105 34-35 0.827889632076122 1105 36-37 0.9007433095750486 1105 38-39 0.8834109874152816 1105 40-41 0.7945350626212075 1105 42-43 0.8110677859871558 1105 44-45 0.8991830007355333 1105 46-47 0.9633291391554835 1105 48-49 0.9894748877068267 1105 50-51 0.9609857365227725 1105 52-53 0.8622509142424235 1105 54-55 0.9230088324381747 1105 56-57 0.863984635250528 1105 58-59 0.7065432912598375 1105 60-61 0.781664730766618 1105 62-63 0.8045983437058801 1105 64-65 0.7963873778787125 1105 66-67 0.7559620648676031 1105 68-69 0.7767877075814447 1105 70-71 0.7152490991475204 1105 72-73 0.735036324311956 1105 74-75 0.8205244874670647 1105 76-77 0.8137684847420346 1105 78-79 0.7472967548870173 1105 80-81 0.5860051764255303 1105 82-83 0.5858602793275409 1105 84-85 0.4882653382148767 1105 86-87 0.6110516186988839 1105 88-89 0.6645389964449784 1105 90-91 0.6399574011786093 1105 92-93 0.4474004524695836 1105 94-95 0.652598180540263 1105 96-97 0.6534824525498166 1105 98-99 0.6963554017447855 1105 100-101 0.7571131462311271 1105 102-103 0.38709734941447493 1105 104-105 0.20475571319143882 1105 106-107 0.5808125088718263 1105 108-109 0.6696953865840172 1105 110-111 0.5876921377185411 1105 112-113 0.4203937094968708 1105 114-115 0.5502185876423411 1105 116-117 0.31481244851151047 1105 118-119 0.23824780271123203 1105 120-121 0.20440245309709582 1105 122-123 0.31005473949695883 1105 124-125 0.14748028590448214 1106 1 0.41662543476212477 1106 2 0.377553849459872 1106 3 0.5715232167718014 1106 4 0.4215974969281149 1106 5 0.5609775838826394 1106 6 0.636442162526869 1106 7 0.571597501598184 1106 8 0.6073376470505067 1106 9 0.6118721803883389 1106 10-11 0.7841062230569733 1106 12-13 0.7342120790434308 1106 14-15 0.7285938355229291 1106 16-17 0.8892805143236302 1106 18-19 0.8581465806330613 1106 20-21 0.9179024743907149 1106 22-23 0.849186953035705 1106 24-25 0.7892259851708161 1106 26-27 0.8413946952325304 1106 28-29 1.0160914325742354 1106 30-31 0.9253934762270575 1106 32-33 0.8441187194788284 1106 34-35 0.7974481769443074 1106 36-37 0.776254095883381 1106 38-39 0.8095847472956095 1106 40-41 0.7986259638528566 1106 42-43 0.7424007677496576 1106 44-45 0.6305447357407843 1106 46-47 0.4937259435111159 1106 48-49 0.5774290080336968 1106 50-51 0.6794393627933744 1106 52-53 0.6550937902583129 1106 54-55 0.6546096548193425 1106 56-57 0.5226286829130338 1106 58-59 0.5302951507749505 1106 60-61 0.6396421866873858 1106 62-63 0.6411662567519762 1106 64-65 0.5383171943510554 1106 66-67 0.4387155464754535 1106 68-69 0.44838337807312456 1106 70-71 0.5433812494354058 1106 72-73 0.6131282744926416 1106 74-75 0.6157477029249101 1106 76-77 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 144786.0 3 45307.0 4 20825.0 5 15947.0 6 16978.0 7 20036.0 8 22736.0 9 24360.0 10 26108.0 11 28472.0 12 31662.0 13 33877.0 14 35642.0 15 29845.0 16 21547.0 17 18763.0 18 17232.0 19 16257.0 20 15727.0 21 16682.0 22 19649.0 23 26018.0 24 35728.0 25 47150.0 26 56802.0 27 64684.0 28 75331.0 29 87388.0 30 102054.0 31 120330.0 32 146293.0 33 181412.0 34 233159.0 35 315756.0 36 501690.0 37 739799.0 38 279031.0 39 1077.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.52143630420581 19.87728275161384 13.547192781713878 25.054088162466474 2 15.410876429778305 20.629458875751617 38.07097210475312 25.88869258971696 3 19.41165631686349 24.171979076713217 29.201790910140975 27.214573696282322 4 13.169056250685879 16.77415646249335 36.031721889440405 34.02506539738037 5 13.294784489422682 38.47108231486734 33.91130006200754 14.322833133702446 6 32.5905193656549 37.19024742803334 16.382179065203424 13.837054141108343 7 28.48429528370356 32.48540965315663 20.394033558141512 18.6362615049983 8 26.08351163596561 36.42464261552085 18.691552030449873 18.80029371806366 9 25.573234972022362 16.659662860226206 19.58113020534537 38.185971962406065 10-11 25.034854656118384 26.97361695499983 26.796980588574165 21.19454780030762 12-13 25.88289429224815 23.942624746495493 27.61153132467871 22.562949636577645 14-15 22.51919382695116 25.476450681191942 25.68129851745229 26.323056974404608 16-17 22.306290773205188 28.599389109608197 26.24031648200218 22.854003635184437 18-19 22.26120592332243 27.486919490062938 28.304243379302473 21.94763120731216 20-21 22.87494736309385 26.315001250083547 28.645465029703583 22.164586357119028 22-23 22.553660602508245 26.109006872633415 28.577904125299135 22.759428399559198 24-25 22.564518967916527 26.314649600950457 28.654576006424392 22.46625542470862 26-27 22.240397784463745 26.494125638726786 28.795728437298894 22.46974813951058 28-29 22.178098002454195 26.82217977650832 28.57429225181055 22.425429969226933 30-31 22.338193798918635 26.38842288800761 28.514289878827547 22.759093434246207 32-33 22.28243777556809 26.386453948234212 28.59383145515354 22.73727682104416 34-35 22.702316192445835 26.39755344953715 28.534806140577647 22.365324217439372 36-37 22.680151671932098 26.10108941149778 28.57251685339182 22.646242063178303 38-39 22.37158085222241 26.57118537790074 28.474618689049375 22.58261508082747 40-41 22.577582271309684 26.47110672306347 28.366578294565116 22.58473271106173 42-43 22.392839624638047 26.393158105566528 28.055130753885337 23.15887151591009 44-45 22.50327270127113 26.51482066147068 28.105147216553817 22.876759420704374 46-47 22.909990914812514 26.834222623808195 27.57839119714499 22.677395264234296 48-49 22.793180566854172 26.839479139680616 27.572523375075424 22.794816918389785 50-51 22.69846333665647 27.052865344288218 27.565072707896455 22.683598611158857 52-53 23.01270125934526 26.9904270619178 27.18579931908478 22.81107235965216 54-55 22.75184673857442 26.99666404483876 27.293984830067046 22.95750438651977 56-57 22.78261256986048 27.081107668918218 27.249709268467626 22.88657049275368 58-59 22.956740652653817 26.769686197364845 27.325179262948968 22.94839388703237 60-61 22.75751208699335 27.031013657339926 27.56179630047248 22.649677955194246 62-63 22.648577887540082 26.958271683708546 27.74255116689676 22.650599261854605 64-65 22.478034983965063 27.1455338557029 27.504858884036654 22.871572276295385 66-67 22.548292970017656 27.080255435709294 27.31411606813819 23.057335526134857 68-69 22.65886933781284 26.84212306699702 27.56172312387088 22.937284471319266 70-71 22.78711765773595 26.69387042303102 27.349868266898415 23.16914365233462 72-73 22.780043673886976 26.824924918317734 27.392080395155165 23.002951012640125 74-75 22.45753825933025 27.431137743489874 27.138839196883062 22.972484800296808 76-77 22.76561469754484 26.922122291152583 27.224546511491997 23.087716499810583 78-79 22.834943317882303 27.018148370862665 27.30990235035422 22.837005960900814 80-81 22.920736128626558 27.055357608675173 27.119176397966328 22.904729864731944 82-83 22.84384262071822 26.962242580235653 27.384050583408253 22.809864215637877 84-85 22.692075514247957 27.176163701292722 27.247021906502372 22.884738877956952 86-87 22.720786682868884 27.21505854372308 27.134547087651057 22.92960768575698 88-89 22.722904333015826 27.196330063794306 26.972246251828345 23.10851935136152 90-91 22.700652340118918 27.0706848471181 27.140580395694336 23.08808241706865 92-93 22.706954935608152 27.13539340305358 27.21072625378727 22.946925407550996 94-95 22.6764791142614 27.014177406250845 27.222831267743075 23.086512211744683 96-97 22.641207933008005 26.934110069674688 27.403677826957146 23.02100417036016 98-99 22.557466759733362 27.094413193029236 26.985615365025513 23.362504682211885 100-101 22.34572065552779 26.927316962693926 27.311448579785587 23.4155138019927 102-103 22.4039123959919 26.6066166410213 27.439905284044734 23.549565678942063 104-105 22.656509333425007 26.734105675684965 27.183045137337135 23.42633985355289 106-107 22.49363371508582 26.85494111444296 27.238348371581466 23.413076798889755 108-109 22.361082939406963 26.795716373012464 27.180390201976795 23.66281048560378 110-111 22.313498070808393 27.009713819045828 27.122499232352386 23.55428887779339 112-113 22.49019769893277 26.906675273173867 27.076119321503235 23.527007706390123 114-115 22.27763834366576 27.088458980794393 26.986647327402846 23.647255348137 116-117 22.27706195969004 26.981123331791824 27.08718624430168 23.65462846421645 118-119 22.184892207519862 26.929548993925394 27.129505677540454 23.756053121014293 120-121 22.00411373333953 27.291386052529592 27.094473663002283 23.610026551128595 122-123 21.96083915583881 27.117027512808 26.966110591095173 23.95602274025802 124-125 22.009556482198068 27.059877350961177 27.060661165116368 23.869905001724394 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 80.0 1 81.0 2 158.5 3 292.0 4 278.5 5 303.0 6 746.5 7 1556.0 8 2376.5 9 2782.5 10 2454.5 11 1959.0 12 1916.0 13 2115.5 14 2419.5 15 2696.0 16 2978.5 17 3338.5 18 3744.0 19 4215.5 20 4904.0 21 5887.0 22 7055.5 23 8617.0 24 10693.0 25 13329.5 26 17117.0 27 21702.5 28 26389.5 29 31895.0 30 38236.5 31 46003.0 32 55778.5 33 62792.5 34 68206.0 35 75080.5 36 82216.0 37 89584.5 38 96750.0 39 102452.5 40 107484.0 41 112828.0 42 115901.5 43 119360.5 44 124139.0 45 127813.0 46 131228.0 47 138959.0 48 148833.0 49 148187.5 50 138950.0 51 132312.5 52 129719.5 53 128723.0 54 127506.5 55 119660.5 56 108803.0 57 99560.5 58 91449.5 59 82849.5 60 69430.5 61 57147.0 62 46270.5 63 34156.5 64 24362.0 65 17823.5 66 12857.5 67 9588.0 68 7498.5 69 6033.5 70 4726.5 71 3208.5 72 2194.5 73 1740.0 74 1302.0 75 843.0 76 597.0 77 367.5 78 205.5 79 121.5 80 83.0 81 55.5 82 34.5 83 26.5 84 20.5 85 12.0 86 5.5 87 3.0 88 3.0 89 2.0 90 3.0 91 3.5 92 2.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06209881907737326 2 0.07692223071718911 3 0.0 4 0.008002992184019318 5 0.030196857106712063 6 3.3002029624821925E-4 7 8.250507406205481E-5 8 0.0 9 6.05037209788402E-4 10-11 0.05831733651619575 12-13 0.009804352967707515 14-15 0.007439207511261943 16-17 0.05325702530705639 18-19 1.3750845677009137E-4 20-21 0.010533147788588998 22-23 0.013434576226437926 24-25 0.0 26-27 0.0 28-29 0.05377955744278273 30-31 0.0 32-33 0.003533967338991348 34-35 0.0 36-37 6.875422838504568E-5 38-39 0.0 40-41 0.0 42-43 0.0035614690303453662 44-45 0.0 46-47 4.1252537031027406E-5 48-49 5.500338270803654E-5 50-51 4.1252537031027405E-4 52-53 1.2375761109308223E-4 54-55 0.0 56-57 0.0014713404874399775 58-59 2.750169135401827E-5 60-61 0.0 62-63 0.0 64-65 4.675287530183106E-4 66-67 0.0 68-69 6.325389011424202E-4 70-71 0.0 72-73 0.002750169135401827 74-75 0.0036164724130534026 76-77 6.187880554654111E-4 78-79 7.837982035895207E-4 80-81 0.0017463574009801603 82-83 1.925118394781279E-4 84-85 1.3750845677009137E-4 86-87 3.162694505712101E-4 88-89 1.3750845677009135E-5 90-91 0.0 92-93 0.007590466813709043 94-95 0.00588536194975991 96-97 0.006682910999026441 98-99 0.0010450642714526944 100-101 4.537779073413015E-4 102-103 1.3750845677009137E-4 104-105 4.1252537031027405E-4 106-107 0.005197819665909454 108-109 0.0055690924991887 110-111 8.663032776515756E-4 112-113 0.0036164724130534026 114-115 3.850236789562558E-4 116-117 0.0024889030675386537 118-119 0.002818923363786873 120-121 0.014108367664611373 122-123 9.21306660359612E-4 124-125 0.002035125160197352 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3636140.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.61104523598158 #Duplication Level Percentage of deduplicated Percentage of total 1 75.32201227686583 38.874477828864826 2 12.583297824482061 12.988743064743446 3 4.6560808953247514 7.20915605132986 4 2.3349701564239043 4.820410014714445 5 1.3413251621001288 3.4613596808655034 6 0.8364725278712168 2.5902732884770336 7 0.5650976335183674 2.041569566838382 8 0.3946534247806371 1.629478060711081 9 0.2970062979471392 1.37959249308491 >10 1.5051936985813141 13.975060111522303 >50 0.10699585440827863 3.805885677047787 >100 0.05281379611706341 4.695673896455986 >500 0.0026308174750660908 0.9111895367242625 >1k 0.0012885636482171227 1.0697741378211811 >5k 1.610704560271403E-4 0.5473565907988859 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7902 0.21731836507945237 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6614 0.18189618661547685 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5237 0.1440263576209937 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4591 0.1262602650062979 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.750169135401827E-5 0.0 0.0 0.0 0.0 3 2.750169135401827E-5 0.0 0.0 0.0 0.0 4 2.750169135401827E-5 0.0 0.0 0.0 0.0 5 1.1000676541607308E-4 0.0 0.0 0.0 0.0 6 1.1000676541607308E-4 0.0 0.0 0.0 0.0 7 1.925118394781279E-4 0.0 0.0 0.0 0.0 8 1.925118394781279E-4 0.0 0.0 0.0 0.0 9 1.925118394781279E-4 0.0 0.0 0.0 0.0 10-11 2.0626268515513702E-4 0.0 0.0 0.0 0.0 12-13 2.7501691354018273E-4 0.0 0.0 0.0 6.875422838504568E-5 14-15 2.887677592171919E-4 0.0 0.0 2.750169135401827E-5 9.625591973906394E-5 16-17 3.02518604894201E-4 0.0 0.0 2.750169135401827E-5 1.1000676541607308E-4 18-19 3.5752198760223753E-4 0.0 0.0 2.750169135401827E-5 1.7876099380111877E-4 20-21 3.850236789562558E-4 0.0 0.0 5.500338270803654E-5 2.2001353083214617E-4 22-23 3.850236789562558E-4 0.0 0.0 1.512593024471005E-4 2.4751522218616445E-4 24-25 4.950304443723289E-4 0.0 0.0 1.6501014812410962E-4 2.4751522218616445E-4 26-27 4.950304443723289E-4 0.0 0.0 2.887677592171918E-4 2.612660678631736E-4 28-29 5.637846727573747E-4 0.0 0.0 6.600405924964386E-4 3.02518604894201E-4 30-31 6.05037209788402E-4 0.0 0.0 0.002103879388582398 3.162694505712101E-4 32-33 6.187880554654111E-4 0.0 0.0 0.00525282304861749 3.3002029624821925E-4 34-35 6.462897468194294E-4 0.0 0.0 0.008814292078962857 3.3002029624821925E-4 36-37 6.875422838504568E-4 0.0 0.0 0.015208435318772105 3.3002029624821925E-4 38-39 7.150439752044751E-4 0.0 0.0 0.02626411524308745 3.3002029624821925E-4 40-41 7.287948208814842E-4 0.0 0.0 0.03949242878437024 3.3002029624821925E-4 42-43 7.700473579125116E-4 0.0 0.0 0.052376971183727805 3.3002029624821925E-4 44-45 7.975490492665299E-4 0.0 0.0 0.0672966387432827 3.3002029624821925E-4 46-47 8.525524319745665E-4 0.0 0.0 0.08573652279615196 3.3002029624821925E-4 48-49 9.350575060366212E-4 0.0 0.0 0.1054964880340141 3.712728332792467E-4 50-51 9.350575060366212E-4 0.0 2.750169135401827E-5 0.12887292568492964 4.125253703102741E-4 52-53 9.350575060366212E-4 0.0 2.750169135401827E-5 0.1530606632307887 4.675287530183106E-4 54-55 9.350575060366212E-4 0.0 2.750169135401827E-5 0.17947603777632326 4.950304443723289E-4 56-57 9.900608887446578E-4 0.0 2.750169135401827E-5 0.20927412035840204 4.950304443723289E-4 58-59 9.900608887446578E-4 0.0 2.750169135401827E-5 0.24377499216201798 4.950304443723289E-4 60-61 9.900608887446578E-4 0.0 2.750169135401827E-5 0.28167232284785515 5.225321357263472E-4 62-63 9.900608887446578E-4 0.0 2.750169135401827E-5 0.3215910278482127 5.500338270803655E-4 64-65 9.900608887446578E-4 2.750169135401827E-5 2.750169135401827E-5 0.3635723597001216 5.500338270803655E-4 66-67 0.0010450642714526944 2.750169135401827E-5 2.750169135401827E-5 0.4137079430384969 5.500338270803655E-4 68-69 0.0010450642714526944 2.750169135401827E-5 2.750169135401827E-5 0.47406040471488997 5.500338270803655E-4 70-71 0.0010725659628067125 2.750169135401827E-5 2.750169135401827E-5 0.5406695011743222 5.637846727573747E-4 72-73 0.0010863168084837217 2.750169135401827E-5 2.750169135401827E-5 0.6115688614849814 6.05037209788402E-4 74-75 0.001100067654160731 2.750169135401827E-5 2.750169135401827E-5 0.6834170301473541 6.462897468194294E-4 76-77 0.001100067654160731 2.750169135401827E-5 2.750169135401827E-5 0.7634607028332242 6.600405924964385E-4 78-79 0.001100067654160731 2.750169135401827E-5 2.750169135401827E-5 0.8607754376894179 6.600405924964385E-4 80-81 0.0012925794936388588 2.750169135401827E-5 2.750169135401827E-5 0.9694758727661752 6.600405924964385E-4 82-83 0.0013475828763468954 2.750169135401827E-5 2.750169135401827E-5 1.0901807961189611 6.600405924964385E-4 84-85 0.0013750845677009136 2.750169135401827E-5 2.750169135401827E-5 1.2110782313112256 6.600405924964385E-4 86-87 0.0014163371047319412 2.750169135401827E-5 2.750169135401827E-5 1.3358671558300834 6.600405924964385E-4 88-89 0.0014300879504089501 2.750169135401827E-5 2.750169135401827E-5 1.4852013398824027 6.600405924964385E-4 90-91 0.0014850913331169867 2.750169135401827E-5 2.750169135401827E-5 1.6530991655986842 6.600405924964385E-4 92-93 0.0014850913331169867 2.750169135401827E-5 2.750169135401827E-5 1.8381992992569043 7.975490492665299E-4 94-95 0.0014850913331169867 2.750169135401827E-5 2.750169135401827E-5 2.0237119582854346 7.975490492665299E-4 96-97 0.0015125930244710049 2.750169135401827E-5 2.750169135401827E-5 2.2155362554797122 9.21306660359612E-4 98-99 0.0016638523269181054 2.750169135401827E-5 2.750169135401827E-5 2.430681986942197 0.0010313134257756852 100-101 0.001828862475042215 2.750169135401827E-5 2.750169135401827E-5 2.6854164031087913 0.0010450642714526944 102-103 0.0019526200861352974 2.750169135401827E-5 2.750169135401827E-5 2.964393560203953 0.0010725659628067125 104-105 0.0023788963021225806 2.750169135401827E-5 2.750169135401827E-5 3.241349342984593 0.001100067654160731 106-107 0.002502653913215663 2.750169135401827E-5 2.750169135401827E-5 3.5152111854879076 0.001100067654160731 108-109 0.002530155604569681 2.750169135401827E-5 2.750169135401827E-5 3.8178948005302327 0.001100067654160731 110-111 0.002598909832954727 2.750169135401827E-5 2.750169135401827E-5 4.158654507252196 0.0011550710368687675 112-113 0.002640162369985754 2.750169135401827E-5 2.750169135401827E-5 4.533640068864235 0.0011688218825457765 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 13355 0.0 27.065023 2 GTACATG 14000 0.0 27.022444 1 ACATGGG 13690 0.0 25.12126 3 GTATCAA 21760 0.0 24.011517 1 GGTATCA 17460 0.0 22.964117 1 GGACCGA 1175 0.0 21.792757 6 AGAGTAC 18300 0.0 21.69931 10-11 GAGTACA 10740 0.0 21.301289 1 GAGTACT 12620 0.0 21.020931 12-13 TCAACGC 25065 0.0 20.749828 4 ATCAACG 25200 0.0 20.683336 3 CAACGCA 25030 0.0 20.5388 5 TATCAAC 25225 0.0 20.396057 2 GTACTTT 12980 0.0 20.323227 14-15 AACGCAG 25530 0.0 20.176548 6 AGTACTT 13385 0.0 20.175419 12-13 AGTACAT 10675 0.0 19.85847 2 ACCGAGT 1405 0.0 19.497057 8 CATGGGG 8275 0.0 19.416304 4 AGGACCG 1455 0.0 19.242434 5 >>END_MODULE