FastQCFastQC Report
Thu 26 May 2016
SRR1364620_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364620_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2590519
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44820.17301552314420393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26240.1012924437149467No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83600.043.895381
GTACATG142400.035.0154571
GGTATCA52650.034.283771
ATCAACG105450.034.2336773
TACATGG144250.034.0694922
TCAACGC106400.033.760064
CAACGCA108150.033.43545
ACGCATA2900.032.829967
TATCAAC111000.032.736662
AACGCAG112000.031.458366
ACATGGG148450.031.2998283
ACGCAGA128350.026.6111977
TACCTGG23800.026.2808762
CATGGGA95950.025.8271144
CGCAGAG125700.025.4665538
CATGGGG57300.024.8468194
CGCATAG3252.3646862E-1123.8003438
GAGTACA114500.022.68431
AGTACAT105800.022.6343422
GCAGAGT133700.022.1617649
Produced by FastQC (version 0.11.5)