##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364620_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2590519 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.49707568251767 30.0 25.0 31.0 2.0 34.0 2 25.275674102370992 30.0 24.0 31.0 2.0 34.0 3 25.819167124425647 31.0 25.0 31.0 2.0 34.0 4 29.12610137196446 35.0 28.0 37.0 2.0 37.0 5 29.246119792983567 35.0 28.0 37.0 2.0 37.0 6 29.426021967026685 35.0 30.0 37.0 2.0 37.0 7 29.207385469861446 35.0 29.0 37.0 2.0 37.0 8 29.03076063136383 35.0 28.0 37.0 2.0 37.0 9 29.843208638886647 35.0 27.0 39.0 2.0 39.0 10-11 29.79546222204894 35.0 27.5 39.0 2.0 39.0 12-13 29.75694696699773 35.0 27.0 39.0 2.0 39.0 14-15 30.31068793550636 36.5 27.0 40.0 2.0 41.0 16-17 30.69004531524378 37.0 27.0 40.0 2.0 41.0 18-19 30.525681147291333 37.0 27.0 40.0 2.0 41.0 20-21 30.43199065515443 37.0 27.0 40.0 2.0 41.0 22-23 30.49540150062594 37.0 27.0 40.0 2.0 41.0 24-25 30.58515841806217 37.0 27.0 40.0 2.0 41.0 26-27 30.391471361530257 37.0 27.0 40.0 2.0 41.0 28-29 30.281433373003633 37.0 27.0 40.0 2.0 41.0 30-31 30.137197797043758 37.0 27.0 40.0 2.0 41.0 32-33 30.02445648922089 36.0 27.0 40.0 2.0 41.0 34-35 30.0564112828356 36.0 27.0 40.0 2.0 41.0 36-37 29.981368405327274 36.0 27.0 40.0 2.0 41.0 38-39 29.685063881021527 36.0 26.0 40.0 2.0 41.0 40-41 29.54070149649549 36.0 25.5 40.0 2.0 41.0 42-43 29.038232107156908 35.0 24.0 40.0 2.0 41.0 44-45 28.88448936294233 35.0 24.0 40.0 2.0 41.0 46-47 28.83051735964878 35.0 24.0 40.0 2.0 41.0 48-49 28.752908201020723 35.0 24.0 40.0 2.0 41.0 50-51 28.51409563102992 35.0 24.0 40.0 2.0 41.0 52-53 28.412067813438156 35.0 23.5 39.0 2.0 41.0 54-55 28.233700852995096 35.0 23.0 39.0 2.0 41.0 56-57 28.002117529344506 34.0 22.5 39.0 2.0 40.5 58-59 27.795328465068195 34.0 22.0 39.0 2.0 40.0 60-61 27.500497004654278 34.0 21.0 39.0 2.0 40.0 62-63 27.322349884328197 34.0 21.0 38.0 2.0 40.0 64-65 27.101959684526538 33.5 20.5 38.0 2.0 40.0 66-67 26.837291098810702 33.0 20.0 38.0 2.0 40.0 68-69 26.59432665809438 33.0 18.5 37.0 2.0 40.0 70-71 26.17085089898974 33.0 17.5 36.5 2.0 39.0 72-73 25.94179699125928 32.0 16.5 36.0 2.0 39.0 74-75 25.90089553483298 33.0 17.5 36.0 2.0 39.0 76-77 25.655907368369043 32.0 14.0 35.5 2.0 38.5 78-79 25.37435741640961 32.0 12.5 35.0 2.0 37.5 80-81 24.98214160946127 32.0 8.0 35.0 2.0 37.0 82-83 25.08885941388579 32.0 10.0 35.0 2.0 36.5 84-85 24.933970567287865 32.0 7.0 35.0 2.0 36.0 86-87 24.81369544095218 32.0 7.0 35.0 2.0 36.0 88-89 24.65230577347628 32.0 6.0 35.0 2.0 36.0 90-91 24.5248955904203 32.0 2.0 35.0 2.0 35.0 92-93 24.407211450678417 32.0 2.0 35.0 2.0 35.0 94-95 24.301188101689277 32.0 2.0 35.0 2.0 35.0 96-97 24.190797288110993 32.0 2.0 35.0 2.0 35.0 98-99 24.096774237131633 32.0 2.0 35.0 2.0 35.0 100-101 23.995298239464756 32.0 2.0 35.0 2.0 35.0 102-103 22.920807181881315 30.0 2.0 34.0 2.0 35.0 104-105 23.713788819923728 31.5 2.0 34.5 2.0 35.0 106-107 23.866533501587906 32.0 2.0 35.0 2.0 35.0 108-109 23.81758095578531 32.0 2.0 35.0 2.0 35.0 110-111 23.744370336600504 32.0 2.0 35.0 2.0 35.0 112-113 23.651469647587994 32.0 2.0 35.0 2.0 35.0 114-115 23.54731175490317 32.0 2.0 35.0 2.0 35.0 116-117 23.43576615342331 32.0 2.0 35.0 2.0 35.0 118-119 23.308526978570704 32.0 2.0 35.0 2.0 35.0 120-121 23.118425303964187 32.0 2.0 35.0 2.0 35.0 122-123 22.90497541226295 32.0 2.0 35.0 2.0 35.0 124-125 22.00175177252126 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1980508685283056 1101 2 1.6536927865327087 1101 3 1.1680910343148483 1101 4 1.428028805379661 1101 5 1.113446967750967 1101 6 1.0724292919968939 1101 7 1.1378745573315712 1101 8 1.4140609878868275 1101 9 1.6196863184574575 1101 10-11 1.6819243243055269 1101 12-13 1.6657998764490216 1101 14-15 2.004618275631934 1101 16-17 1.5662165207195855 1101 18-19 1.5881275240676906 1101 20-21 1.7592635672662382 1101 22-23 1.4904955467071588 1101 24-25 1.6443457632517635 1101 26-27 1.8429475956678907 1101 28-29 1.8132979163373832 1101 30-31 1.8585835566159936 1101 32-33 1.8195300342423302 1101 34-35 1.676501748088203 1101 36-37 1.505662480343478 1101 38-39 1.4005680858420106 1101 40-41 1.554700508150244 1101 42-43 1.7761105824807224 1101 44-45 1.5283843785074716 1101 46-47 1.2035924060864431 1101 48-49 1.1300905892967386 1101 50-51 1.2255526985663607 1101 52-53 1.1115665460863262 1101 54-55 1.010506524355982 1101 56-57 1.092064124926118 1101 58-59 1.0264966258952768 1101 60-61 1.0334105361665493 1101 62-63 0.9261190980730483 1101 64-65 0.9481902113059384 1101 66-67 0.965197284630964 1101 68-69 0.7998414107458558 1101 70-71 0.558230069256215 1101 72-73 0.5846015182254014 1101 74-75 0.6487764778422758 1101 76-77 0.7733304927330202 1101 78-79 0.7767541455344933 1101 80-81 0.8823075106720601 1101 82-83 0.9672318791639007 1101 84-85 1.0200519510568675 1101 86-87 0.9654398932570132 1101 88-89 0.9718848813412748 1101 90-91 0.8990883065900519 1101 92-93 0.8839806714821918 1101 94-95 0.7774976218315004 1101 96-97 0.8331698574034263 1101 98-99 0.9127755104256075 1101 100-101 0.8151246690137732 1101 102-103 0.7009688246053578 1101 104-105 0.9433569647756599 1101 106-107 0.9077908387436864 1101 108-109 0.8261457997505524 1101 110-111 0.9296712658725532 1101 112-113 0.8465642868783512 1101 114-115 0.8693387967440671 1101 116-117 0.9070312513617509 1101 118-119 0.8420550256273245 1101 120-121 0.9606258747784544 1101 122-123 0.9115783959181165 1101 124-125 0.8387427000485204 1102 1 0.7235200827511967 1102 2 1.2705438710215589 1102 3 0.9876372948496837 1102 4 0.9041245240737226 1102 5 0.7009489359922121 1102 6 0.5306694573112161 1102 7 0.6049708767445914 1102 8 0.7157413449564949 1102 9 1.1786226029175495 1102 10-11 1.1349136152791353 1102 12-13 1.2454755930923866 1102 14-15 1.3987749900014563 1102 16-17 1.0619907878403865 1102 18-19 0.8990999808343538 1102 20-21 1.3141063239383541 1102 22-23 1.3012987450767568 1102 24-25 0.9627806661134493 1102 26-27 1.2855970477964718 1102 28-29 0.7392267709541471 1102 30-31 1.0226402077395882 1102 32-33 1.2266294269407183 1102 34-35 1.1184146881376087 1102 36-37 0.8823438689716561 1102 38-39 1.2663256493049388 1102 40-41 1.164010209892524 1102 42-43 1.3370160741946506 1102 44-45 0.989292174840422 1102 46-47 0.8133302218623761 1102 48-49 0.725947249997617 1102 50-51 0.6608993029172332 1102 52-53 0.6389671897512379 1102 54-55 0.6101684974659101 1102 56-57 0.6270701493658422 1102 58-59 0.5437637079177762 1102 60-61 0.6240272246166967 1102 62-63 0.452024706397939 1102 64-65 0.33517076294340953 1102 66-67 0.2526775314929246 1102 68-69 0.1962270850132093 1102 70-71 0.5324218897355024 1102 72-73 0.14050778067569425 1102 74-75 0.3113770789476362 1102 76-77 0.13740789232787876 1102 78-79 0.1368733274582219 1102 80-81 0.3112985415640317 1102 82-83 0.3819051883773952 1102 84-85 0.19759107196477288 1102 86-87 0.32987454330061183 1102 88-89 0.24817951487717949 1102 90-91 0.4227257626936165 1102 92-93 0.5085813824364607 1102 94-95 0.4816003059950553 1102 96-97 0.2539845278077131 1102 98-99 0.3576915622626977 1102 100-101 0.4613830027300594 1102 102-103 0.38662938154759274 1102 104-105 0.4779767585274399 1102 106-107 0.41213214757684824 1102 108-109 0.459580205118133 1102 110-111 0.48201647792993185 1102 112-113 0.3082645202520773 1102 114-115 0.36027688294703 1102 116-117 0.4969258143514743 1102 118-119 0.49496328923213184 1102 120-121 0.5135307159550138 1102 122-123 0.4392022564254603 1102 124-125 0.4362286021971542 1103 1 0.8793868049373401 1103 2 1.2305969378950188 1103 3 0.9171863587145062 1103 4 0.7097446318663323 1103 5 0.7801204611377663 1103 6 0.8650613143431123 1103 7 0.7736478145138541 1103 8 1.0325164782798772 1103 9 1.4460017834929566 1103 10-11 1.4551551459871384 1103 12-13 1.352388061518834 1103 14-15 1.572302637292374 1103 16-17 1.2555331633305151 1103 18-19 1.183779335926534 1103 20-21 1.5362613866360917 1103 22-23 1.2579115574341628 1103 24-25 1.1933017617285238 1103 26-27 1.402844663598355 1103 28-29 1.3391323206546772 1103 30-31 1.2901130406795183 1103 32-33 1.4575345812434861 1103 34-35 1.2357933332566695 1103 36-37 1.1515615546537425 1103 38-39 1.2020640635520294 1103 40-41 1.3418943719967906 1103 42-43 1.518102265237495 1103 44-45 1.255408308392056 1103 46-47 1.1707916380331156 1103 48-49 1.1365038873149764 1103 50-51 0.9719911445993787 1103 52-53 0.8735959704667451 1103 54-55 0.8874218080035021 1103 56-57 0.8504352384101352 1103 58-59 0.8814576507306171 1103 60-61 0.9216047781187555 1103 62-63 0.8142166168914784 1103 64-65 0.6928674649312967 1103 66-67 0.6949625819927547 1103 68-69 0.7193738128604927 1103 70-71 0.7486586899491812 1103 72-73 0.706764728746716 1103 74-75 0.6804953797325766 1103 76-77 0.6487195650871982 1103 78-79 0.6350424867266646 1103 80-81 0.7532555988051541 1103 82-83 0.774086223857573 1103 84-85 0.8354133353156428 1103 86-87 0.7869393438074255 1103 88-89 0.7497899785760751 1103 90-91 0.7731588418846691 1103 92-93 0.8326065946609056 1103 94-95 0.7806893162680488 1103 96-97 0.7346846863237921 1103 98-99 0.6963904831396768 1103 100-101 0.6277855109335668 1103 102-103 0.5247901373011779 1103 104-105 0.6189623089755116 1103 106-107 0.7609532931888481 1103 108-109 0.8463530265300037 1103 110-111 0.704929893799136 1103 112-113 0.7446926232688398 1103 114-115 0.8727811534738521 1103 116-117 0.9643234524220432 1103 118-119 1.0053553419680696 1103 120-121 1.1016755445071418 1103 122-123 0.8973819610341458 1103 124-125 0.7633435025193336 1104 1 0.87798651022133 1104 2 1.4392382260369487 1104 3 0.8783851127429294 1104 4 1.0741733505865732 1104 5 0.5083328666264535 1104 6 0.7190541428798909 1104 7 0.5359245693591141 1104 8 0.7461093708555069 1104 9 1.137146756594415 1104 10-11 1.1894687058104658 1104 12-13 1.0719779342547362 1104 14-15 1.1244249539241267 1104 16-17 0.7442053322509565 1104 18-19 0.8585183907235638 1104 20-21 1.121467583868899 1104 22-23 1.1218828990380416 1104 24-25 1.0252720486698266 1104 26-27 1.137626704374469 1104 28-29 1.1445997689506733 1104 30-31 1.327188548584136 1104 32-33 1.2656379793606405 1104 34-35 1.1694837457803438 1104 36-37 1.1506861685683027 1104 38-39 1.2154414609460176 1104 40-41 1.1516936287665445 1104 42-43 1.3487335304510104 1104 44-45 1.1176739997784857 1104 46-47 0.8541567586217909 1104 48-49 0.731707978856619 1104 50-51 0.818412569916088 1104 52-53 0.6691785816974054 1104 54-55 0.7057591074253153 1104 56-57 0.613592931259042 1104 58-59 0.6171644391922371 1104 60-61 0.621155052555121 1104 62-63 0.49524619690602734 1104 64-65 0.43884378791807777 1104 66-67 0.38743951074428296 1104 68-69 0.23764285397153628 1104 70-71 0.4063658728998263 1104 72-73 0.3578511987624218 1104 74-75 0.35113489812486876 1104 76-77 0.32868721456451766 1104 78-79 0.3769777198658453 1104 80-81 0.5356891478961252 1104 82-83 0.5715733449214895 1104 84-85 0.5316278154047467 1104 86-87 0.4596960609723233 1104 88-89 0.6164308843422575 1104 90-91 0.5306493385148556 1104 92-93 0.5448150407234138 1104 94-95 0.4943817395955534 1104 96-97 0.5450081451163449 1104 98-99 0.5529702027294512 1104 100-101 0.5762195974502191 1104 102-103 0.5695445537511681 1104 104-105 0.6563785027701634 1104 106-107 0.6397323072610419 1104 108-109 0.6401178387954225 1104 110-111 0.5851273725014714 1104 112-113 0.5171879359357092 1104 114-115 0.45771762842571917 1104 116-117 0.3628449866976666 1104 118-119 0.3404782099557728 1104 120-121 0.4024386001456399 1104 122-123 0.4606364793365998 1104 124-125 0.21117858753333252 1105 1 1.0317564568354278 1105 2 1.442213963074824 1105 3 0.9031366890044374 1105 4 1.1169874910027353 1105 5 0.8646377972432475 1105 6 0.8571586201243235 1105 7 0.8866841142631401 1105 8 0.8391965196111961 1105 9 1.0509527221498125 1105 10-11 0.9910207674543337 1105 12-13 1.161672869749978 1105 14-15 1.4392707826983404 1105 16-17 1.0185662095711052 1105 18-19 1.2022768068135115 1105 20-21 1.0483398416663334 1105 22-23 1.148628944341418 1105 24-25 0.7884278881089131 1105 26-27 1.2032992336412995 1105 28-29 1.3122969497625263 1105 30-31 1.2700742690476048 1105 32-33 1.3511360272125046 1105 34-35 1.0405362629510932 1105 36-37 0.6746550965565845 1105 38-39 0.969952677525967 1105 40-41 1.0732755805145509 1105 42-43 1.2068251729178137 1105 44-45 0.9785586299014319 1105 46-47 0.4328596073576243 1105 48-49 0.5709544088955383 1105 50-51 0.733310414935378 1105 52-53 0.5540429124037658 1105 54-55 0.6332320335853296 1105 56-57 0.6945868801384592 1105 58-59 0.6261818707901057 1105 60-61 0.5997626022975808 1105 62-63 0.4515235035110088 1105 64-65 0.22905980922934788 1105 66-67 0.26564878225523003 1105 68-69 0.1597986387781738 1105 70-71 0.4450288130180482 1105 72-73 0.4163900578214843 1105 74-75 0.4186480022173811 1105 76-77 0.42129430954956604 1105 78-79 0.457421785474029 1105 80-81 0.6238870774033387 1105 82-83 0.5595768098497658 1105 84-85 0.5192606838236529 1105 86-87 0.6084876258764425 1105 88-89 0.7186988811166373 1105 90-91 0.48432419774012203 1105 92-93 0.5953237957653741 1105 94-95 0.4679560828135152 1105 96-97 0.6085413524103167 1105 98-99 0.6550508332956113 1105 100-101 0.6857632318468063 1105 102-103 0.5154160143765623 1105 104-105 0.5726740920080324 1105 106-107 0.6569086330981868 1105 108-109 0.7455956841840603 1105 110-111 0.7739735621576251 1105 112-113 0.626565268394927 1105 114-115 0.7463229543967529 1105 116-117 0.6497523785446617 1105 118-119 0.6410285164361014 1105 120-121 0.5899848645943813 1105 122-123 0.5465083939499351 1105 124-125 0.5227326190231665 1106 1 0.29195078289801657 1106 2 -1.9994604958152067 1106 3 -0.2625304751657218 1106 4 0.3245003931264172 1106 5 0.5657393478956934 1106 6 0.6391299627244109 1106 7 0.6417775310332026 1106 8 0.7392837325726624 1106 9 1.2196760862324574 1106 10-11 1.3863689599582152 1106 12-13 1.4079543308517977 1106 14-15 1.5086365556569277 1106 16-17 1.1312837174981922 1106 18-19 1.1760181379650199 1106 20-21 1.3520323815115631 1106 22-23 1.0490885356692736 1106 24-25 1.1589923148110657 1106 26-27 1.2069110040943904 1106 28-29 1.29498772436947 1106 30-31 1.381322867945908 1106 32-33 1.4847074729096974 1106 34-35 1.2515994968436708 1106 36-37 1.0587086860742438 1106 38-39 1.4109243378090035 1106 40-41 1.4650936710601883 1106 42-43 1.6360920146098046 1106 44-45 1.5260432402602966 1106 46-47 1.4097279427588916 1106 48-49 1.1354706466902194 1106 50-51 1.1677193747934425 1106 52-53 1.1635015476857582 1106 54-55 1.1210551860791398 1106 56-57 1.1904090496133222 1106 58-59 1.075151049549703 1106 60-61 1.205258817075233 1106 62-63 1.0847817617692677 1106 64-65 1.1778424241796266 1106 66-67 1.1044943643642853 1106 68-69 0.9636702499102654 1106 70-71 1.0663376218268539 1106 72-73 0.8469546044039937 1106 74-75 0.8799922057023011 1106 76-77 0.6860954130482781 1106 78-79 0.7525436288743101 1106 80-81 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122-123 -1.7680618987434826 2216 124-125 -1.6628525672297059 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 292118.0 3 37556.0 4 22169.0 5 17816.0 6 17166.0 7 18741.0 8 20275.0 9 21262.0 10 22491.0 11 23466.0 12 24961.0 13 26078.0 14 26134.0 15 21739.0 16 17833.0 17 15220.0 18 13684.0 19 12780.0 20 12610.0 21 13373.0 22 16601.0 23 23832.0 24 34798.0 25 45269.0 26 49910.0 27 52830.0 28 60220.0 29 68357.0 30 78472.0 31 90433.0 32 108494.0 33 134930.0 34 173845.0 35 233916.0 36 324994.0 37 336967.0 38 79075.0 39 104.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36300868550667 18.905909332161443 14.089112710425859 22.641969271906028 2 13.624593703354979 20.67243521796153 41.37234709914161 24.330623979541883 3 19.3481251570581 26.630587347922262 27.537797519827556 26.483489975192082 4 11.677444057001251 17.162008508608682 36.59959946280165 34.56094797158842 5 13.200316167816798 39.79179551486287 32.56865438527938 14.43923393204096 6 31.246237136617673 37.342037123307385 17.564465878470926 13.847259861604014 7 28.527615076197733 33.86932966347179 19.969535609560573 17.63351965076991 8 25.29770757911179 37.69259364274162 19.0138292592637 17.99586951888289 9 26.013742075561687 18.357697028352558 19.389068307700878 36.23949258838488 10-11 23.936339760452583 29.140464214697705 26.578985061678733 20.34421096317098 12-13 24.874532900057673 26.166974054697217 27.08304774507243 21.87544530017268 14-15 22.039167454695587 27.131516709113868 25.12448923156061 25.704826604629936 16-17 21.973218316251224 30.575739502548544 25.293350591051055 22.15769159014918 18-19 22.015830791686554 28.87351997258657 27.677044423834523 21.433604811892355 20-21 23.041572407760018 27.634452245129687 27.726166749460457 21.59780859764984 22-23 22.667769106540174 27.555387933118645 27.448296536984923 22.32854642335625 24-25 22.497360207006185 27.526523634366427 27.66369617433077 22.312419984296618 26-27 22.288176701763916 27.66618855151231 27.706860265573525 22.338774481150246 28-29 22.157968801299024 27.636554739647966 27.890098807358715 22.315377651694295 30-31 22.145716554685464 27.524525932495784 27.837619652604385 22.492137860214363 32-33 22.111826995449164 27.45204137912896 27.74421398452421 22.691917640897667 34-35 22.713269090878864 27.355211388656542 27.603867537778314 22.327651982686277 36-37 22.600322404981096 27.119636837214482 27.569066829151456 22.710973928652965 38-39 22.302564915727512 27.37183156331459 27.767301191958726 22.558302328999172 40-41 22.5074378138417 27.234216380713942 27.90763300930989 22.350712796134463 42-43 22.205934722695318 27.186541975041028 27.842314897965505 22.765208404298146 44-45 22.41852076563779 27.56336264083863 27.45967076136483 22.558445832158753 46-47 22.580777366523883 27.63285943120145 26.95786690297978 22.828496299294887 48-49 22.599178719949922 27.603608744983475 26.986015117569174 22.81119741749743 50-51 22.562131843164124 27.703026145757047 26.978215864703113 22.756626146375716 52-53 22.84754765404826 27.671351337971984 26.79194575326212 22.68915525471764 54-55 22.652348280103745 27.65256289735611 26.88026948192942 22.81481934061073 56-57 22.65948266944777 28.003436161012335 26.69617913667131 22.640902032868585 58-59 22.698915826977675 27.464460124824484 26.881336327141593 22.955287721056244 60-61 22.69232234033689 27.556978937517023 27.102937619873114 22.64776110227297 62-63 22.66553546112863 27.624070167218157 27.050271377213747 22.66012299443946 64-65 22.583434828738707 27.584199306113554 26.83491483180821 22.997451033339527 66-67 22.5239603955038 27.911606728875725 26.70174422054329 22.862688655077186 68-69 22.67380966968765 27.639791948891375 26.923101454390203 22.763296927030776 70-71 22.579653197289826 27.619647708131396 26.981088496250848 22.819610598327934 72-73 22.80025599831808 27.640803905829408 26.641351535081704 22.917588560770806 74-75 22.537666481995316 27.86526424653799 26.696443392111902 22.900625879354795 76-77 22.73163554947716 27.678617332208194 26.818218892506636 22.77152822580801 78-79 22.7674807433967 27.698466236916623 26.77809241236675 22.755960607319924 80-81 22.538456452878158 27.901153567933772 26.82173998268701 22.73864999650106 82-83 22.6676610700546 27.954662628739378 26.683821284988007 22.693855016218016 84-85 22.59955945501694 27.766066593577538 26.719907561322962 22.91446639008256 86-87 22.64058032097844 27.708857357737642 26.94855876774594 22.702003553537974 88-89 22.655057403177086 27.686090536634257 26.791635669621243 22.867216390567414 90-91 22.531271873977282 27.82212364387382 26.88616629992969 22.760438182219207 92-93 22.57470188065329 27.79275698730509 26.857089062333273 22.77545206970835 94-95 22.50670771826984 27.69258551821172 26.864921625505822 22.93578513801262 96-97 22.730232327950187 27.558863012808814 26.823017355773242 22.887887303467753 98-99 22.482111219259473 27.65219358062686 26.716090575273032 23.149604624840634 100-101 22.305842496923994 27.703144889424223 26.985738853998072 23.00527375965371 102-103 22.4576973310866 27.504918352924573 27.032331101102002 23.00505321488683 104-105 22.323406502207746 27.601913237153507 27.082108472692035 22.992571787946716 106-107 22.276019321340897 27.805439795150953 26.93972527915379 22.97881560435436 108-109 22.40603526914046 27.857428809413765 26.589739292440584 23.14679662900519 110-111 22.19080277796674 27.86307753198805 26.944329348612563 23.001790341432642 112-113 22.315486756616473 27.818274644117764 26.922351526197875 22.943887073067888 114-115 22.11177034839007 27.94366922228466 26.837641091678382 23.10691933764689 116-117 21.96299039637908 27.97120053860821 27.019631024775936 23.04617804023677 118-119 22.16379225897125 27.99455601138956 26.680104033973144 23.161547695666044 120-121 21.97065375922033 28.01507826520737 26.92918582507056 23.085082150501737 122-123 22.11063190985631 27.935197870050594 26.83456811882233 23.119602101270768 124-125 22.110161222904786 27.846058977409776 26.85780077572665 23.18597902395879 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5847.0 1 4323.0 2 2218.5 3 1398.0 4 1081.5 5 955.5 6 946.5 7 1097.5 8 1350.5 9 1471.5 10 1471.5 11 1523.0 12 1628.0 13 1739.5 14 1883.0 15 2101.5 16 2341.5 17 2573.5 18 2825.5 19 3206.0 20 3754.5 21 4548.0 22 5681.0 23 6997.0 24 8532.0 25 10869.5 26 14204.0 27 17750.0 28 20755.5 29 24090.0 30 28737.5 31 33960.0 32 39865.0 33 45810.5 34 51284.5 35 56503.5 36 62139.0 37 68210.5 38 72853.5 39 75759.5 40 77388.5 41 80005.5 42 82901.0 43 85620.5 44 88711.5 45 91458.0 46 93101.5 47 94460.5 48 97599.0 49 97998.5 50 96095.0 51 93869.5 52 90880.0 53 88976.5 54 86263.5 55 80574.0 56 73369.5 57 66683.0 58 60906.5 59 54397.0 60 46420.5 61 38636.5 62 31181.5 63 23980.0 64 18436.0 65 14439.5 66 10854.0 67 8138.0 68 6185.0 69 4658.5 70 3542.0 71 2681.0 72 2101.5 73 1692.0 74 1306.0 75 1028.0 76 764.0 77 497.5 78 350.0 79 271.5 80 194.0 81 123.0 82 96.5 83 83.0 84 60.0 85 44.5 86 34.5 87 26.0 88 17.5 89 11.5 90 9.5 91 8.5 92 8.5 93 6.5 94 4.0 95 1.5 96 0.0 97 0.5 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2132005208222754 2 0.2512237895186254 3 0.3040317403578202 4 0.28905404669875034 5 0.32217482288298216 6 0.2335825369356488 7 0.226286701622339 8 0.20644511775439592 9 0.20536425326353522 10-11 0.14859956634172533 12-13 0.17303482429582645 14-15 0.1912358102758559 16-17 0.1635386576975502 18-19 0.1901163434817502 20-21 0.18442250375310892 22-23 0.20629070854141585 24-25 0.19644712121393437 26-27 0.24757587186197053 28-29 0.17672134425572636 30-31 0.15722718111698852 32-33 0.19536625672307364 34-35 0.15500754868039957 36-37 0.1552005601966247 38-39 0.18756859146757848 40-41 0.2091472789815477 42-43 0.27326570467153494 44-45 0.17340154617665415 46-47 0.15904148936950474 48-49 0.161762951748279 50-51 0.16695496153473494 52-53 0.18729837534486332 54-55 0.17448241066751488 56-57 0.1734594496315217 58-59 0.1485030605836128 60-61 0.161762951748279 62-63 0.15047177804910908 64-65 0.1460904166307987 66-67 0.1347027371735162 68-69 0.13736629609742296 70-71 0.13902619513695905 72-73 0.11588411434156631 74-75 0.11733170071325474 76-77 0.13811904101070094 78-79 0.14452702334937517 80-81 0.15525846365149223 82-83 0.1560884131712603 84-85 0.14371637498122963 86-87 0.16846045136129092 88-89 0.16409839109460306 90-91 0.1532318427311284 92-93 0.15328974618599595 94-95 0.173922677270462 96-97 0.16116461604798113 98-99 0.1563972315972205 100-101 0.1374241995522905 102-103 0.17531236018728294 104-105 0.1347220383251387 106-107 0.14045448035702499 108-109 0.15145613678185724 110-111 0.12750340761831896 112-113 0.11874068478169818 114-115 0.11646314889024167 116-117 0.12003386194040654 118-119 0.10463154294564139 120-121 0.09864818594266245 122-123 0.12458893372331954 124-125 0.12115332873451229 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2590519.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.907112588329305 #Duplication Level Percentage of deduplicated Percentage of total 1 81.3843004292457 51.19651350024975 2 10.102426557374338 12.71028969720151 3 3.5839953429333438 6.763763956618672 4 1.661276289412846 4.180243783136635 5 0.9125633793322654 2.8703363623820537 6 0.5594172022490121 2.1114792555436077 7 0.37993749922362363 1.6730539728132137 8 0.25562696235832255 1.286460328135009 9 0.17753190149068088 1.0051217383585003 >10 0.8983240581511944 10.076377603977726 >50 0.05794010031728049 2.499418753933739 >100 0.02583516554813306 2.9410306701301767 >500 5.077614543157161E-4 0.19664174918762167 >1k 3.173509089458412E-4 0.48926862833195706 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4482 0.17301552314420393 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2624 0.1012924437149467 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.860230324502542E-5 2 0.0 0.0 0.0 0.0 3.860230324502542E-5 3 0.0 0.0 0.0 0.0 3.860230324502542E-5 4 0.0 0.0 0.0 3.860230324502542E-5 1.1580690973507625E-4 5 0.0 0.0 0.0 3.860230324502542E-5 1.1580690973507625E-4 6 0.0 0.0 0.0 3.860230324502542E-5 1.1580690973507625E-4 7 0.0 0.0 0.0 3.860230324502542E-5 1.1580690973507625E-4 8 0.0 0.0 0.0 3.860230324502542E-5 1.5440921298010167E-4 9 0.0 0.0 0.0 3.860230324502542E-5 1.9301151622512708E-4 10-11 0.0 0.0 0.0 3.860230324502542E-5 1.9301151622512708E-4 12-13 0.0 3.860230324502542E-5 0.0 3.860230324502542E-5 1.9301151622512708E-4 14-15 0.0 3.860230324502542E-5 0.0 3.860230324502542E-5 2.5091497109266524E-4 16-17 0.0 3.860230324502542E-5 0.0 7.720460649005084E-5 3.0881842596020334E-4 18-19 0.0 3.860230324502542E-5 0.0 1.1580690973507625E-4 4.63227638940305E-4 20-21 0.0 3.860230324502542E-5 0.0 1.3510806135758895E-4 4.8252879056281776E-4 22-23 0.0 3.860230324502542E-5 0.0 2.5091497109266524E-4 5.597333970528686E-4 24-25 0.0 3.860230324502542E-5 0.0 5.018299421853304E-4 6.176368519204067E-4 26-27 0.0 3.860230324502542E-5 0.0 7.527449132779956E-4 6.176368519204067E-4 28-29 0.0 3.860230324502542E-5 0.0 0.0012159725522183007 6.176368519204067E-4 30-31 0.0 3.860230324502542E-5 0.0 0.0026056554690392156 6.176368519204067E-4 32-33 3.860230324502542E-5 3.860230324502542E-5 0.0 0.0052692143929459695 6.562391551654321E-4 34-35 3.860230324502542E-5 3.860230324502542E-5 0.0 0.008473205562283079 7.334437616554829E-4 36-37 7.720460649005084E-5 3.860230324502542E-5 0.0 0.015016295962314886 7.527449132779956E-4 38-39 7.720460649005084E-5 3.860230324502542E-5 0.0 0.026693492693935075 7.720460649005083E-4 40-41 7.720460649005084E-5 3.860230324502542E-5 0.0 0.041671186353004944 7.720460649005083E-4 42-43 7.720460649005084E-5 3.860230324502542E-5 0.0 0.05705420419614757 7.913472165230211E-4 44-45 7.720460649005084E-5 3.860230324502542E-5 0.0 0.07340227962041583 8.106483681455338E-4 46-47 7.720460649005084E-5 3.860230324502542E-5 0.0 0.09368778997567669 8.106483681455338E-4 48-49 7.720460649005084E-5 3.860230324502542E-5 0.0 0.11673336501295686 8.492506713905592E-4 50-51 7.720460649005084E-5 3.860230324502542E-5 0.0 0.14612901893404373 8.492506713905592E-4 52-53 7.720460649005084E-5 3.860230324502542E-5 0.0 0.1740577853318196 8.685518230130719E-4 54-55 7.720460649005084E-5 3.860230324502542E-5 0.0 0.20443779798565462 8.878529746355847E-4 56-57 7.720460649005084E-5 3.860230324502542E-5 0.0 0.2390447628448199 8.878529746355847E-4 58-59 7.720460649005084E-5 5.790345486753813E-5 0.0 0.28150729641434785 8.878529746355847E-4 60-61 7.720460649005084E-5 7.720460649005084E-5 0.0 0.3298952835319872 8.878529746355847E-4 62-63 7.720460649005084E-5 7.720460649005084E-5 0.0 0.38098543187677836 9.2645527788061E-4 64-65 7.720460649005084E-5 7.720460649005084E-5 0.0 0.431303534156669 9.2645527788061E-4 66-67 7.720460649005084E-5 7.720460649005084E-5 0.0 0.4889946763563595 9.2645527788061E-4 68-69 7.720460649005084E-5 7.720460649005084E-5 0.0 0.5590385555944581 9.2645527788061E-4 70-71 7.720460649005084E-5 1.1580690973507625E-4 0.0 0.6397366705281837 9.2645527788061E-4 72-73 1.1580690973507625E-4 1.1580690973507625E-4 0.0 0.7252214710642926 0.001003659884370661 74-75 1.1580690973507625E-4 1.1580690973507625E-4 0.0 0.808660349528415 0.001003659884370661 76-77 1.1580690973507625E-4 1.1580690973507625E-4 0.0 0.8971947320208807 0.0010229610359931735 78-79 1.1580690973507625E-4 1.1580690973507625E-4 0.0 1.0084272688214215 0.0010422621876156862 80-81 1.1580690973507625E-4 1.1580690973507625E-4 0.0 1.1389030537896074 0.0010808644908607116 82-83 1.1580690973507625E-4 1.1580690973507625E-4 0.0 1.2777362374103414 0.0011580690973507625 84-85 1.1580690973507625E-4 1.1580690973507625E-4 0.0 1.4154306530853469 0.0011580690973507625 86-87 1.1580690973507625E-4 1.1580690973507625E-4 0.0 1.5607490236512451 0.0011580690973507625 88-89 1.1580690973507625E-4 1.1580690973507625E-4 0.0 1.7379335955459119 0.001196671400595788 90-91 1.1580690973507625E-4 1.1580690973507625E-4 0.0 1.9443787133003076 0.001196671400595788 92-93 1.1580690973507625E-4 1.1580690973507625E-4 0.0 2.1672491110854617 0.001196671400595788 94-95 1.1580690973507625E-4 1.1580690973507625E-4 0.0 2.3868190119431665 0.0013896829168209151 96-97 1.1580690973507625E-4 1.3510806135758895E-4 0.0 2.608261124508255 0.0013896829168209151 98-99 1.1580690973507625E-4 1.5440921298010167E-4 0.0 2.854003386966087 0.0013896829168209151 100-101 1.1580690973507625E-4 1.5440921298010167E-4 0.0 3.1492531033356634 0.0013896829168209151 102-103 1.1580690973507625E-4 1.5440921298010167E-4 0.0 3.4715437331283807 0.0014861886749334785 104-105 1.1580690973507625E-4 1.5440921298010167E-4 0.0 3.791576128181264 0.0015054898265559912 106-107 1.1580690973507625E-4 1.5440921298010167E-4 0.0 4.106918343389877 0.001524790978178504 108-109 1.1580690973507625E-4 1.5440921298010167E-4 0.0 4.447525766072358 0.0015440921298010167 110-111 1.1580690973507625E-4 1.5440921298010167E-4 0.0 4.8449171768282735 0.0016792001911586057 112-113 1.1580690973507625E-4 1.5440921298010167E-4 0.0 5.283169125569046 0.0017757059492711693 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8360 0.0 43.89538 1 GTACATG 14240 0.0 35.015457 1 GGTATCA 5265 0.0 34.28377 1 ATCAACG 10545 0.0 34.233677 3 TACATGG 14425 0.0 34.069492 2 TCAACGC 10640 0.0 33.76006 4 CAACGCA 10815 0.0 33.4354 5 ACGCATA 290 0.0 32.82996 7 TATCAAC 11100 0.0 32.73666 2 AACGCAG 11200 0.0 31.45836 6 ACATGGG 14845 0.0 31.299828 3 ACGCAGA 12835 0.0 26.611197 7 TACCTGG 2380 0.0 26.280876 2 CATGGGA 9595 0.0 25.827114 4 CGCAGAG 12570 0.0 25.466553 8 CATGGGG 5730 0.0 24.846819 4 CGCATAG 325 2.3646862E-11 23.800343 8 GAGTACA 11450 0.0 22.6843 1 AGTACAT 10580 0.0 22.634342 2 GCAGAGT 13370 0.0 22.161764 9 >>END_MODULE