FastQCFastQC Report
Thu 26 May 2016
SRR1364620_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364620_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2590519
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107340.4143571230321028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91720.3540603253633731No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63700.2458966716708119No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79250.052.02471
GTATCAA129600.044.3634261
ATCAACG166500.034.1625823
TCAACGC166900.034.1533244
TATCAAC168400.033.731222
CAACGCA169600.033.2583735
GTACATG143200.032.9938431
AACGCAG174050.032.6387636
TACATGG146150.031.3702282
ACATGGG147650.029.981033
TACCTGG25200.029.2986032
ACGCAGA203850.027.5466447
CGCAGAG205200.027.2203188
CATGGGG55750.024.760934
GAGTACT124200.024.72010412-13
GCAGAGT222400.024.5542959
CATGGGA98600.024.1986584
GTACTTT129850.023.41515414-15
AGTACTT130000.023.36523212-13
GTACCTG32250.023.2778871
Produced by FastQC (version 0.11.5)