##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364620_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2590519 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.41834744311854 31.0 30.0 33.0 26.0 34.0 2 29.50909103542572 31.0 30.0 34.0 25.0 34.0 3 29.61478105352634 31.0 30.0 34.0 26.0 34.0 4 33.31056325006688 35.0 33.0 37.0 28.0 37.0 5 32.65976277340564 35.0 33.0 37.0 26.0 37.0 6 32.67774835853356 35.0 33.0 37.0 25.0 37.0 7 32.46673079795979 35.0 33.0 37.0 24.0 37.0 8 32.40738207285876 35.0 33.0 37.0 23.0 37.0 9 33.670587631281606 38.0 33.0 39.0 22.0 39.0 10-11 33.471050781715945 37.0 33.0 39.0 20.5 39.0 12-13 33.325463739119456 37.0 33.0 39.0 18.0 39.0 14-15 34.28775932544791 38.0 33.0 41.0 18.0 41.0 16-17 34.211085500627476 38.0 33.0 41.0 17.5 41.0 18-19 34.26453946101148 38.5 33.0 41.0 18.0 41.0 20-21 34.28783228380105 39.0 33.0 41.0 18.0 41.0 22-23 34.27865188404331 39.0 33.0 41.0 18.0 41.0 24-25 34.266752724068034 39.0 33.0 41.0 19.0 41.0 26-27 34.16364693715815 39.0 33.0 41.0 18.5 41.0 28-29 33.92104304195414 38.0 33.0 41.0 17.0 41.0 30-31 33.83727102561302 38.0 33.0 40.5 17.0 41.0 32-33 33.64000051727086 38.0 33.0 40.0 16.0 41.0 34-35 33.48841043049674 38.0 33.0 40.0 15.5 41.0 36-37 33.30767714886476 38.0 32.0 40.0 14.0 41.0 38-39 33.16557666629737 38.0 32.0 40.0 9.0 41.0 40-41 32.97906191770838 38.0 32.0 40.0 9.0 41.0 42-43 32.77985357374333 38.0 31.5 40.0 7.5 41.0 44-45 32.54712491975546 38.0 31.0 40.0 2.0 41.0 46-47 32.237327925407996 38.0 31.0 40.0 2.0 41.0 48-49 31.93356755924199 38.0 30.0 40.0 2.0 41.0 50-51 31.72002116178264 37.5 30.0 40.0 2.0 41.0 52-53 31.550762607801758 37.0 30.0 40.0 2.0 41.0 54-55 31.328542465814763 37.0 30.0 40.0 2.0 41.0 56-57 31.099189583245675 37.0 29.0 40.0 2.0 41.0 58-59 30.9094262578271 36.0 29.0 40.0 2.0 41.0 60-61 30.687553729580827 36.0 29.0 40.0 2.0 41.0 62-63 30.419381598822476 35.5 28.5 39.0 2.0 41.0 64-65 30.149005469560347 35.0 28.0 39.0 2.0 41.0 66-67 29.871863514608464 35.0 28.0 39.0 2.0 40.5 68-69 29.556621279365253 35.0 28.0 38.5 2.0 40.0 70-71 29.298379205093653 35.0 28.0 37.5 2.0 40.0 72-73 29.142014013408122 35.0 28.0 37.0 2.0 39.5 74-75 28.859835036917314 34.0 28.0 37.0 2.0 39.0 76-77 28.574977253592813 34.0 27.5 36.0 2.0 39.0 78-79 28.283166616419336 34.0 27.0 36.0 2.0 38.5 80-81 28.01694737618215 34.0 26.5 35.5 2.0 37.0 82-83 27.77490533750187 34.0 26.0 35.0 2.0 37.0 84-85 27.757971472125856 34.0 27.0 35.0 2.0 36.5 86-87 27.618352731634086 34.0 27.0 35.0 2.0 36.0 88-89 27.480308772103196 34.0 27.0 35.0 2.0 36.0 90-91 27.33231217373816 34.0 26.0 35.0 2.0 36.0 92-93 27.204903341762787 34.0 26.0 35.0 2.0 35.0 94-95 27.121878859024 34.0 26.0 35.0 2.0 35.0 96-97 27.040349057466862 34.0 26.0 35.0 2.0 35.0 98-99 26.952007107456073 34.0 25.5 35.0 2.0 35.0 100-101 26.85909638184472 34.0 25.0 35.0 2.0 35.0 102-103 25.686819706784625 31.5 23.5 34.0 2.0 35.0 104-105 26.603654518650508 33.5 25.0 35.0 2.0 35.0 106-107 26.77618867107325 34.0 25.0 35.0 2.0 35.0 108-109 26.786889036521252 34.0 25.0 35.0 2.0 35.0 110-111 26.7295457010738 34.0 25.0 35.0 2.0 35.0 112-113 26.682746584757727 34.0 25.0 35.0 2.0 35.0 114-115 26.605107702356168 34.0 25.0 35.0 2.0 35.0 116-117 26.491835805875194 34.0 25.0 35.0 2.0 35.0 118-119 26.389731555723003 34.0 25.0 35.0 2.0 35.0 120-121 26.25333108925277 34.0 24.5 35.0 2.0 35.0 122-123 26.073751244441752 34.0 24.0 35.0 2.0 35.0 124-125 25.277988117439016 33.0 20.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7846496729835124 1101 2 0.7160455107368335 1101 3 0.6436490531506536 1101 4 0.7662071296754434 1101 5 1.017969955568283 1101 6 0.9662783844613259 1101 7 1.012682968094147 1101 8 1.176503832792477 1101 9 1.2083618452979366 1101 10-11 1.2320112736612998 1101 12-13 1.1995151433038984 1101 14-15 1.326995296671555 1101 16-17 1.3801804178188135 1101 18-19 1.3939498623551145 1101 20-21 1.4535199942752257 1101 22-23 1.3961813326060977 1101 24-25 1.2902635588832823 1101 26-27 1.46123015669329 1101 28-29 1.5230156503481496 1101 30-31 1.5033860530759782 1101 32-33 1.4622080585378399 1101 34-35 1.4128235541381784 1101 36-37 1.380647244321878 1101 38-39 1.4860186163028075 1101 40-41 1.590039714397669 1101 42-43 1.601750593324006 1101 44-45 1.5620004598984139 1101 46-47 1.6497158060670358 1101 48-49 1.7058748714451752 1101 50-51 1.62846752157148 1101 52-53 1.559559721706897 1101 54-55 1.4384742770972387 1101 56-57 1.5593717573549668 1101 58-59 1.6249192437963664 1101 60-61 1.4577357129257784 1101 62-63 1.4991250351542327 1101 64-65 1.3728599529830667 1101 66-67 1.3855946906796603 1101 68-69 1.4363335611821988 1101 70-71 1.423377461570464 1101 72-73 1.4422929579397739 1101 74-75 1.387895307116473 1101 76-77 1.424777225280149 1101 78-79 1.4142953788175632 1101 80-81 1.4155679469492597 1101 82-83 1.3440810205134497 1101 84-85 1.4401206533044615 1101 86-87 1.4630872767878138 1101 88-89 1.455160068510434 1101 90-91 1.5179122665076257 1101 92-93 1.4635105987122579 1101 94-95 1.5029633376912557 1101 96-97 1.5064663847960134 1101 98-99 1.5882569307788472 1101 100-101 1.5778711579729787 1101 102-103 1.5342706660709737 1101 104-105 1.531179485909334 1101 106-107 1.5155698495879655 1101 108-109 1.4561295828656498 1101 110-111 1.4153748803009059 1101 112-113 1.4693147851632204 1101 114-115 1.3837916823080114 1101 116-117 1.427269991907199 1101 118-119 1.487724485468675 1101 120-121 1.5260044146812177 1101 122-123 1.6175561364724373 1101 124-125 1.3976900687804488 1102 1 0.5764835107800117 1102 2 -0.34566680463661825 1102 3 0.22527865892141818 1102 4 0.46146970237271745 1102 5 0.8537703352646133 1102 6 0.7338189157408763 1102 7 0.7048799144877407 1102 8 0.5775398349436358 1102 9 0.9555242313762733 1102 10-11 0.9281671277317116 1102 12-13 1.0767918688459943 1102 14-15 1.0968992354586646 1102 16-17 1.3023342512765552 1102 18-19 1.2285066447867976 1102 20-21 1.1595861586453395 1102 22-23 1.1938117012471636 1102 24-25 1.2643504639966068 1102 26-27 1.2789707413240805 1102 28-29 1.428858439174526 1102 30-31 1.2193423105789307 1102 32-33 1.2344759913373622 1102 34-35 1.104533149509173 1102 36-37 1.045503439544916 1102 38-39 0.9561586543386866 1102 40-41 0.8955405640016778 1102 42-43 0.8407078456771657 1102 44-45 0.7031639640619787 1102 46-47 0.7114769048431384 1102 48-49 0.7510120425073978 1102 50-51 0.7616355502403849 1102 52-53 0.7390572512794975 1102 54-55 0.850865564974935 1102 56-57 0.5325639665834601 1102 58-59 0.8252515988396283 1102 60-61 0.7055418053172637 1102 62-63 0.797629917013527 1102 64-65 0.7293955903282168 1102 66-67 0.6970238277157534 1102 68-69 0.5113788221057831 1102 70-71 0.4410912187798175 1102 72-73 0.4712920687984372 1102 74-75 0.6037066979236911 1102 76-77 0.5821522407535298 1102 78-79 0.6319173100689852 1102 80-81 0.576100980488917 1102 82-83 0.5156682557944876 1102 84-85 0.49146711661816767 1102 86-87 0.43676794104801786 1102 88-89 0.5840635514797832 1102 90-91 0.4471138871378031 1102 92-93 0.6535930571528681 1102 94-95 0.41675309461190935 1102 96-97 0.3578370535095097 1102 98-99 0.4212567498593529 1102 100-101 0.23292896182009315 1102 102-103 0.2695681282162248 1102 104-105 0.20006669440434877 1102 106-107 0.45748658466869685 1102 108-109 0.4997744786106182 1102 110-111 0.3954254356381881 1102 112-113 0.4500192588979459 1102 114-115 0.5769115113190821 1102 116-117 0.4846359269056286 1102 118-119 0.4592077575916029 1102 120-121 0.5194348014556311 1102 122-123 0.5067010979749504 1102 124-125 0.33855292366245493 1103 1 0.29842187114548935 1103 2 -0.9714934809876965 1103 3 0.03297507743241823 1103 4 0.29759402315684724 1103 5 0.6331432129938932 1103 6 0.7037865342809368 1103 7 0.5517446496058867 1103 8 0.6992505318705184 1103 9 0.7286165211618894 1103 10-11 0.9154688330236809 1103 12-13 0.8434070267121712 1103 14-15 0.9299640006883507 1103 16-17 0.9863123265440805 1103 18-19 1.0015898003171273 1103 20-21 1.0199884900432608 1103 22-23 0.9707903541775877 1103 24-25 0.9023766207240413 1103 26-27 0.8571575517534242 1103 28-29 1.0168497702413077 1103 30-31 0.8885143879794555 1103 32-33 0.9098973692324179 1103 34-35 0.8286465125466904 1103 36-37 1.049684303679065 1103 38-39 1.0164278355923315 1103 40-41 1.0193175974601445 1103 42-43 0.9628116305555849 1103 44-45 1.0773138746197688 1103 46-47 0.9609153581260443 1103 48-49 0.9365826279375611 1103 50-51 1.0025931099705403 1103 52-53 0.9712025644721152 1103 54-55 0.9996003236300588 1103 56-57 0.9641477645564152 1103 58-59 0.9334288991443351 1103 60-61 0.8112478171061248 1103 62-63 0.9621460440136929 1103 64-65 1.0132383736260948 1103 66-67 0.9718051147974194 1103 68-69 0.9772714078832614 1103 70-71 1.0149608861858326 1103 72-73 1.0135673970909203 1103 74-75 0.8988019388087025 1103 76-77 0.8064879756610601 1103 78-79 0.8818690665022828 1103 80-81 0.9226801388901329 1103 82-83 0.9369446241321775 1103 84-85 0.996118447395169 1103 86-87 1.0309093556984799 1103 88-89 0.9024045271400389 1103 90-91 0.8944582651506607 1103 92-93 0.8966533854443028 1103 94-95 0.7966646204252079 1103 96-97 0.8333091632684031 1103 98-99 0.8776850922565984 1103 100-101 0.9121650986978516 1103 102-103 0.8952174740250634 1103 104-105 0.8623912300849561 1103 106-107 0.8563542099912063 1103 108-109 0.8298105557692494 1103 110-111 0.8791202649987504 1103 112-113 0.8989372486142457 1103 114-115 0.8362393183229742 1103 116-117 0.8722893115251793 1103 118-119 0.8943341767346489 1103 120-121 0.8598413031135479 1103 122-123 0.8900077319850794 1103 124-125 0.9441548889342215 1104 1 0.36811439380186073 1104 2 -0.688762349080779 1104 3 0.088371309759399 1104 4 0.26308229013667983 1104 5 0.6206806707766006 1104 6 0.6331261608649754 1104 7 0.7134679892441795 1104 8 0.7395968262073822 1104 9 0.841849407172127 1104 10-11 0.9273986939249141 1104 12-13 0.8103798766208072 1104 14-15 0.7918137019850633 1104 16-17 0.9251681428244467 1104 18-19 0.794163372823057 1104 20-21 0.9262550511629257 1104 22-23 0.8953129346364932 1104 24-25 0.8769287474822107 1104 26-27 0.7612451363924109 1104 28-29 0.9130126477742664 1104 30-31 0.9908901446302067 1104 32-33 1.0199247795573285 1104 34-35 1.0793314678375978 1104 36-37 1.0511904886168963 1104 38-39 1.0106967418422457 1104 40-41 1.0806543822696142 1104 42-43 0.9576316908675864 1104 44-45 0.9023210466886482 1104 46-47 0.9304662160185444 1104 48-49 0.8623607494631926 1104 50-51 0.8729461291945171 1104 52-53 0.6704585872434663 1104 54-55 0.756237944679544 1104 56-57 0.8331187599224776 1104 58-59 0.8059413117192911 1104 60-61 0.7578475498468755 1104 62-63 0.6884070007242755 1104 64-65 0.7081708653290057 1104 66-67 0.6719312745678998 1104 68-69 0.6923308144175273 1104 70-71 0.688026088115155 1104 72-73 0.5949746690539541 1104 74-75 0.5504915490297222 1104 76-77 0.6159769306749787 1104 78-79 0.5164762319492446 1104 80-81 0.5578368432957141 1104 82-83 0.5876758723265425 1104 84-85 0.6435455976480426 1104 86-87 0.704321300155879 1104 88-89 0.6240395246558066 1104 90-91 0.657504818691443 1104 92-93 0.648258949718052 1104 94-95 0.6834148767724137 1104 96-97 0.6703966027499675 1104 98-99 0.7339308892021563 1104 100-101 0.7116583801702134 1104 102-103 0.7058682941039471 1104 104-105 0.6275311596220305 1104 106-107 0.7003821528964913 1104 108-109 0.658388923517407 1104 110-111 0.5624333994442026 1104 112-113 0.6374329170076578 1104 114-115 0.6405064419024171 1104 116-117 0.711020421628195 1104 118-119 0.6260562020824878 1104 120-121 0.6296459543289288 1104 122-123 0.6098326022771268 1104 124-125 0.5209926293802312 1105 1 0.49760618123701406 1105 2 0.38735892417840745 1105 3 0.528592640421131 1105 4 0.500469889648528 1105 5 0.681536214892688 1105 6 0.563575401166176 1105 7 0.5713172483204758 1105 8 0.6024359128759826 1105 9 0.6794140619797062 1105 10-11 0.7689275675577534 1105 12-13 0.8337643913230863 1105 14-15 0.8341431718040937 1105 16-17 0.861619449924973 1105 18-19 0.8384934674526079 1105 20-21 0.865654875261832 1105 22-23 0.7706489049472225 1105 24-25 0.8355143711462176 1105 26-27 0.8252962939077619 1105 28-29 1.0360571332058726 1105 30-31 0.9020256607990973 1105 32-33 0.9555544839111505 1105 34-35 0.9987506287447658 1105 36-37 1.0114037359849206 1105 38-39 1.0648686601490382 1105 40-41 0.9507013931053052 1105 42-43 1.008602609477549 1105 44-45 1.0991190466794265 1105 46-47 1.0405676207649464 1105 48-49 1.0531699560307857 1105 50-51 1.044116798649739 1105 52-53 0.9850470279410999 1105 54-55 0.9581712022969207 1105 56-57 0.9652078008075549 1105 58-59 0.9167080203990743 1105 60-61 0.9836252735051865 1105 62-63 0.9122617199673613 1105 64-65 0.9850487787388822 1105 66-67 0.9960381687209932 1105 68-69 0.8760120256265687 1105 70-71 0.9570557812347005 1105 72-73 0.8296187194266089 1105 74-75 0.8170033404045682 1105 76-77 0.8975137991123532 1105 78-79 0.9890573803694416 1105 80-81 0.7047344513179077 1105 82-83 0.885107704883481 1105 84-85 0.7310835133355624 1105 86-87 0.7789184435714382 1105 88-89 0.8337299164243142 1105 90-91 0.7937314979466557 1105 92-93 0.6674069490162928 1105 94-95 0.7695235961109148 1105 96-97 0.7654371500285819 1105 98-99 0.8622534653297755 1105 100-101 0.8434555029639483 1105 102-103 0.45867147725191515 1105 104-105 0.4219080958036372 1105 106-107 0.77317267439782 1105 108-109 0.8449667124268672 1105 110-111 0.7576818770456697 1105 112-113 0.6551972149345531 1105 114-115 0.723994150371368 1105 116-117 0.44734413118672123 1105 118-119 0.29293505039973056 1105 120-121 0.35067238643002696 1105 122-123 0.3310041758608797 1105 124-125 0.22304986802553373 1106 1 0.6695768045549393 1106 2 0.5973076453546717 1106 3 0.7320101297667634 1106 4 0.5968117404813995 1106 5 0.7799669809881209 1106 6 0.8631601655713013 1106 7 0.8317086277238346 1106 8 0.8624893795162762 1106 9 0.7755880803094115 1106 10-11 0.9043350558844025 1106 12-13 0.8053740998339975 1106 14-15 0.9697222388951374 1106 16-17 1.147819572202934 1106 18-19 1.0892798076850596 1106 20-21 1.1486088621828827 1106 22-23 1.0904383800510615 1106 24-25 1.1005170234991652 1106 26-27 1.0448242050524428 1106 28-29 1.2883891808223495 1106 30-31 1.2465213540971902 1106 32-33 1.1185370772829657 1106 34-35 1.0664571826040188 1106 36-37 1.1141265935730473 1106 38-39 1.1129286962330198 1106 40-41 1.2026782951570567 1106 42-43 1.1181268043310837 1106 44-45 1.0383277164270126 1106 46-47 1.0713989760723521 1106 48-49 1.0996827862262535 1106 50-51 1.1385214577999108 1106 52-53 1.1225258241025244 1106 54-55 1.162331200618592 1106 56-57 1.0056820325243052 1106 58-59 1.0968402484354947 1106 60-61 1.2232687343312811 1106 62-63 1.3008389199218087 1106 64-65 1.2223897874122294 1106 66-67 1.0696765526138705 1106 68-69 1.1475507799497535 1106 70-71 1.178628776385537 1106 72-73 1.2429070077148978 1106 74-75 1.2687211510295668 1106 76-77 1.298303436054681 1106 78-79 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scores pass #Quality Count 2 108428.0 3 33383.0 4 15444.0 5 11896.0 6 12992.0 7 15605.0 8 17194.0 9 18418.0 10 19644.0 11 21665.0 12 25059.0 13 28599.0 14 32556.0 15 26001.0 16 16616.0 17 14158.0 18 13047.0 19 11784.0 20 11648.0 21 12056.0 22 14259.0 23 18764.0 24 25502.0 25 34332.0 26 41082.0 27 45252.0 28 51955.0 29 60508.0 30 70622.0 31 83084.0 32 101217.0 33 125360.0 34 162255.0 35 222337.0 36 351269.0 37 523044.0 38 192785.0 39 699.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.263659732589645 20.039823786504332 13.550941421179996 24.145575059726024 2 15.563710623936133 20.809383883253144 38.34857160959621 25.27833388321451 3 18.94821076394344 25.625984599997143 29.273516233619596 26.152288402439822 4 12.811794009416552 17.09248080940976 35.90802260096389 34.187702580209795 5 13.026718136460854 39.102125735450564 33.25203813975076 14.619117988337822 6 31.814333840700186 37.490576139963764 16.56209865357987 14.132991365756173 7 28.24849248238865 33.47177416708711 20.040169587769547 18.239563762754692 8 26.677356931178657 36.22081135093006 18.419204800273615 18.682626917617668 9 26.60222620685928 16.215525544247164 19.381471952379943 37.80077629651362 10-11 25.01041937451307 27.63082929723395 26.221075372152246 21.137675956100733 12-13 25.96193547403262 24.73213591147914 27.5278596603931 21.77806895409514 14-15 22.82859755883349 25.698070392102284 25.97571959283779 25.49761245622643 16-17 21.9774950475432 29.114092477016314 26.420550774220885 22.487861701219604 18-19 21.82768922188119 27.031380222557893 29.685888955756706 21.455041599804208 20-21 22.71387364522611 25.966431276502146 29.89718900402415 21.4225060742476 22-23 22.28747698045317 25.951107833788257 29.80233110312371 21.959084082634863 24-25 22.07109849416275 26.159063106659318 29.942088824671814 21.82774957450611 26-27 21.865097302895677 26.348040682195347 29.894376377860958 21.89248563704802 28-29 21.693130173727955 26.227414033029206 30.063982066791468 22.01547372645137 30-31 21.758477741332914 26.090486114944532 30.108271740141646 22.042764403580904 32-33 21.701411273666956 26.161334081730185 29.98074447986547 22.156510164737384 34-35 22.17455266685942 26.116504067331682 29.830412361383956 21.87853090442494 36-37 22.17873666047099 25.84092352292884 29.793554563474828 22.186785253125343 38-39 21.950335425128213 26.192690768276698 29.759640782337588 22.097333024257498 40-41 22.052704496666497 26.049393963140204 29.87239236616292 22.02550917403038 42-43 21.96194991715655 26.090746394032184 29.50415915825493 22.443144530556342 44-45 22.136896120043897 26.42318006546179 29.147788531950546 22.292135282543768 46-47 22.353111934927313 26.55649824542847 28.538630393876748 22.551759425767468 48-49 22.466965539084995 26.74098137647946 28.220417218278453 22.571635866157088 50-51 22.524995174676704 26.89706620343563 27.988245512449332 22.589693109438333 52-53 22.659806246796 26.883604476644003 27.87182611005083 22.58476316650917 54-55 22.441086601002848 26.969253452542414 27.922653322105205 22.66700662434953 56-57 22.409474886593895 27.23852321722437 27.805524791070653 22.54647710511108 58-59 22.540506852199666 26.709035276142597 27.968898890318677 22.781558981339064 60-61 22.528226969190342 26.81312123169141 28.205197491313516 22.45345430780473 62-63 22.484394053855617 26.804184798490187 28.261402444838275 22.45001870281592 64-65 22.369803885766697 26.680892976293624 28.152073022041453 22.79723011589823 66-67 22.33118151227611 27.039697450588086 27.961308139411443 22.667812897724353 68-69 22.407351469454284 26.798545841974676 28.21738253485569 22.576720153715353 70-71 22.315605482916745 26.763169851292346 28.109946308056415 22.811278357734494 72-73 22.547697624296788 26.82092144440717 27.861133640592133 22.77024729070391 74-75 22.359288140827672 27.095197652872145 27.782794163063617 22.762720043236566 76-77 22.479889998154796 26.936342840113614 27.923815538455482 22.659951623276108 78-79 22.54787458369403 26.89760730826281 27.86719127733433 22.68732683070883 80-81 22.345355223320563 27.144158065585806 27.74860459631055 22.761882114783074 82-83 22.497629326443054 27.169453410401857 27.622086551732988 22.7108307114221 84-85 22.338344697882118 27.05567470832021 27.684604433409305 22.92137616038837 86-87 22.431276754015347 26.933401634202525 27.84340005535613 22.791921556426 88-89 22.474134516726 26.8569066109558 27.728932977883186 22.940025894435013 90-91 22.238497382184804 27.10646013405036 27.83959507727988 22.815447406484957 92-93 22.32030642111129 27.008543246966603 27.81854860736716 22.852601724554937 94-95 22.272514066767684 26.86848175422003 27.750475326455177 23.10852885255711 96-97 22.465390798920055 26.78415042491928 27.704482330434267 23.0459764457264 98-99 22.276184775830114 26.859564030520218 27.56298592596467 23.301265267684997 100-101 22.104448034909044 26.89745449098988 27.820730603143367 23.177366870957712 102-103 22.34285460613044 26.656379136966972 27.7822942542313 23.218472002671284 104-105 22.184586830568165 26.721878680516763 27.863068080705688 23.23046640820938 106-107 22.17363005673683 26.80352812964579 27.73530225862528 23.287539554992104 108-109 22.260192465639065 26.81744525075449 27.43018512102211 23.49217716258434 110-111 22.072243698277 26.786578713922736 27.683265122089246 23.457912465711015 112-113 22.19069134336471 26.720174366489267 27.704595341425243 23.384538948720778 114-115 21.92069336368009 26.89382212405163 27.556724526751957 23.62875998551632 116-117 21.85221851349935 26.877659177139634 27.687849880300234 23.582272429060783 118-119 21.90339662148364 26.91535024920602 27.437156113644583 23.74409701566576 120-121 21.674026039710114 26.952292962375118 27.649075081820502 23.72460591609427 122-123 21.851379252373935 26.89969173660706 27.44783349955095 23.801095511468052 124-125 21.721377923818583 26.838292063766577 27.491345126402845 23.948984886011996 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 189.0 1 169.5 2 288.5 3 540.5 4 494.0 5 446.5 6 1110.5 7 2604.0 8 4007.0 9 4467.5 10 3725.5 11 2785.5 12 2647.0 13 2778.0 14 2996.5 15 3387.5 16 3755.5 17 4044.0 18 4392.0 19 4784.5 20 5200.5 21 5830.0 22 6797.0 23 7938.0 24 9271.0 25 11269.0 26 14364.0 27 17740.5 28 20799.0 29 24086.0 30 28329.5 31 33244.0 32 38923.0 33 44765.0 34 50247.5 35 55184.5 36 60654.0 37 67042.0 38 71956.5 39 74772.5 40 75793.0 41 78490.0 42 81639.0 43 84558.0 44 87670.5 45 90809.5 46 92810.0 47 93447.5 48 97621.0 49 98786.0 50 96591.5 51 94557.0 52 91494.5 53 89648.0 54 87524.5 55 81711.0 56 74001.0 57 67182.0 58 61429.5 59 55309.0 60 47033.5 61 38775.0 62 31198.0 63 23661.0 64 17838.0 65 13822.5 66 10131.0 67 7475.5 68 5585.5 69 3957.0 70 2943.5 71 2207.0 72 1772.0 73 1516.5 74 1171.5 75 879.5 76 582.5 77 341.5 78 211.5 79 131.5 80 74.5 81 53.5 82 42.0 83 34.0 84 27.5 85 13.0 86 10.0 87 6.5 88 5.0 89 2.5 90 1.0 91 2.5 92 1.5 93 1.0 94 1.5 95 1.0 96 2.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.062304117437471024 2 0.0803313930528979 3 0.0 4 0.007218630706819753 5 0.030109796531119826 6 7.720460649005083E-4 7 7.720460649005084E-5 8 0.0 9 5.404322454303558E-4 10-11 0.06145486676608046 12-13 0.010017297692084096 14-15 0.007720460649005083 16-17 0.05404322454303558 18-19 7.720460649005084E-5 20-21 0.010827946060229628 22-23 0.012700157767613362 24-25 0.0 26-27 0.0 28-29 0.05234472320025447 30-31 0.0 32-33 0.0035900142017873636 34-35 0.0 36-37 1.5440921298010167E-4 38-39 1.930115162251271E-5 40-41 0.0 42-43 0.003512809595297313 44-45 0.0 46-47 1.930115162251271E-5 48-49 1.737103646026144E-4 50-51 7.334437616554829E-4 52-53 2.702161227151779E-4 54-55 1.930115162251271E-5 56-57 0.0019301151622512708 58-59 1.930115162251271E-5 60-61 0.0 62-63 0.0 64-65 5.211310938078431E-4 66-67 0.0 68-69 6.369380035429194E-4 70-71 0.0 72-73 0.003319798079072186 74-75 0.004593674086158025 76-77 6.56239155165432E-4 78-79 0.0010229610359931737 80-81 0.0019301151622512708 82-83 4.053241840727669E-4 84-85 2.702161227151779E-4 86-87 7.720460649005083E-4 88-89 3.860230324502542E-5 90-91 0.0 92-93 0.008782023988243282 94-95 0.007488846829534931 96-97 0.007855568710362673 98-99 0.001524790978178504 100-101 5.98335700297894E-4 102-103 1.5440921298010167E-4 104-105 7.720460649005083E-4 106-107 0.005327117847813508 108-109 0.007199329555197241 110-111 0.0014282852200659406 112-113 0.004362060266687872 114-115 6.56239155165432E-4 116-117 0.003068883107979521 118-119 0.0035514118985423386 120-121 0.015363716691520115 122-123 0.001524790978178504 124-125 0.002740763530396805 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2590519.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.01669664612353 #Duplication Level Percentage of deduplicated Percentage of total 1 75.7236481505106 40.14617682933413 2 12.476846623310088 13.229623850564831 3 4.71510442571534 7.4993778297883384 4 2.2742316544549968 4.822889989090088 5 1.3065071770409444 3.4633347335581472 6 0.8226958032038933 2.616996829829985 7 0.5405190044755979 2.005957246422322 8 0.36308516915177025 1.539966101570068 9 0.2618183084361725 1.2492667651285583 >10 1.3641786321395326 12.968233083333539 >50 0.09803907025284093 3.567725671361577 >100 0.050317039165860916 4.766406163497335 >500 0.0025686091459631737 0.8685896666733377 >1k 2.2016649822359895E-4 0.233099833945758 >5k 1.467776654823993E-4 0.6047133398739728 >10k+ 7.338883274119966E-5 0.417642066028003 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10734 0.4143571230321028 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9172 0.3540603253633731 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6370 0.2458966716708119 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 1.930115162251271E-5 16-17 1.930115162251271E-5 0.0 0.0 3.860230324502542E-5 3.860230324502542E-5 18-19 3.860230324502542E-5 1.930115162251271E-5 0.0 7.720460649005084E-5 7.720460649005084E-5 20-21 3.860230324502542E-5 3.860230324502542E-5 0.0 1.1580690973507626E-4 7.720460649005084E-5 22-23 3.860230324502542E-5 3.860230324502542E-5 0.0 2.3161381947015252E-4 1.3510806135758895E-4 24-25 3.860230324502542E-5 5.790345486753813E-5 0.0 4.63227638940305E-4 2.702161227151779E-4 26-27 3.860230324502542E-5 7.720460649005084E-5 0.0 6.755403067879447E-4 2.702161227151779E-4 28-29 3.860230324502542E-5 7.720460649005084E-5 0.0 0.0011966714005957882 2.702161227151779E-4 30-31 3.860230324502542E-5 7.720460649005084E-5 0.0 0.0025670531657941906 2.702161227151779E-4 32-33 3.860230324502542E-5 7.720460649005084E-5 0.0 0.005249913241323456 2.702161227151779E-4 34-35 3.860230324502542E-5 7.720460649005084E-5 0.0 0.008801325139865795 3.0881842596020334E-4 36-37 3.860230324502542E-5 7.720460649005084E-5 0.0 0.015826944330460422 3.0881842596020334E-4 38-39 3.860230324502542E-5 7.720460649005084E-5 0.0 0.028044573307510964 3.0881842596020334E-4 40-41 3.860230324502542E-5 7.720460649005084E-5 0.0 0.04369780727336878 3.0881842596020334E-4 42-43 3.860230324502542E-5 7.720460649005084E-5 0.0 0.05969846196843181 3.0881842596020334E-4 44-45 3.860230324502542E-5 7.720460649005084E-5 0.0 0.07672207769948802 3.0881842596020334E-4 46-47 3.860230324502542E-5 7.720460649005084E-5 0.0 0.09762522490666928 3.0881842596020334E-4 48-49 3.860230324502542E-5 7.720460649005084E-5 0.0 0.12115332873451226 3.0881842596020334E-4 50-51 3.860230324502542E-5 7.720460649005084E-5 0.0 0.15116661950751953 3.0881842596020334E-4 52-53 3.860230324502542E-5 7.720460649005084E-5 0.0 0.17924979511827552 4.246253356952796E-4 54-55 3.860230324502542E-5 7.720460649005084E-5 0.0 0.21018954116916339 5.404322454303558E-4 56-57 3.860230324502542E-5 7.720460649005084E-5 0.0 0.24543344403187162 5.597333970528685E-4 58-59 3.860230324502542E-5 7.720460649005084E-5 0.0 0.28851361445331997 5.790345486753813E-4 60-61 3.860230324502542E-5 7.720460649005084E-5 0.0 0.33771224993910487 5.790345486753813E-4 62-63 3.860230324502542E-5 7.720460649005084E-5 0.0 0.38899540980012115 6.176368519204067E-4 64-65 3.860230324502542E-5 7.720460649005084E-5 0.0 0.4402592685095149 6.176368519204067E-4 66-67 3.860230324502542E-5 7.720460649005084E-5 0.0 0.49818202452867555 6.562391551654321E-4 68-69 3.860230324502542E-5 7.720460649005084E-5 0.0 0.5692295636511449 6.755403067879448E-4 70-71 3.860230324502542E-5 7.720460649005084E-5 0.0 0.6509313384692411 8.299495197680465E-4 72-73 3.860230324502542E-5 1.1580690973507625E-4 1.930115162251271E-5 0.7368407643410452 8.878529746355847E-4 74-75 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 0.8208007738989755 8.878529746355847E-4 76-77 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 0.9111880669472024 8.878529746355847E-4 78-79 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 1.0246788384875773 8.878529746355847E-4 80-81 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 1.156582908675829 9.071541262580974E-4 82-83 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 1.2964583544841788 9.2645527788061E-4 84-85 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 1.4352722369532902 9.2645527788061E-4 86-87 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 1.5810924374613735 9.2645527788061E-4 88-89 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 1.7605159429442518 9.2645527788061E-4 90-91 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 1.9679454194313957 9.2645527788061E-4 92-93 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 2.192359909346351 9.2645527788061E-4 94-95 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 2.415674233618823 9.650575811256354E-4 96-97 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 2.640069422382156 9.650575811256354E-4 98-99 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 2.8904439612293906 9.650575811256354E-4 100-101 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 3.1898241240461855 9.650575811256354E-4 102-103 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 3.515666165737445 0.0010036598843706607 104-105 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 3.8401571268151287 0.0010422621876156862 106-107 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 4.158394514767117 0.001061563339238199 108-109 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 4.503209588503307 0.0012159725522183007 110-111 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 4.9069317769914065 0.0013703817651984024 112-113 3.860230324502542E-5 1.1580690973507625E-4 3.860230324502542E-5 5.351340793099761 0.0013896829168209151 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7925 0.0 52.0247 1 GTATCAA 12960 0.0 44.363426 1 ATCAACG 16650 0.0 34.162582 3 TCAACGC 16690 0.0 34.153324 4 TATCAAC 16840 0.0 33.73122 2 CAACGCA 16960 0.0 33.258373 5 GTACATG 14320 0.0 32.993843 1 AACGCAG 17405 0.0 32.638763 6 TACATGG 14615 0.0 31.370228 2 ACATGGG 14765 0.0 29.98103 3 TACCTGG 2520 0.0 29.298603 2 ACGCAGA 20385 0.0 27.546644 7 CGCAGAG 20520 0.0 27.220318 8 CATGGGG 5575 0.0 24.76093 4 GAGTACT 12420 0.0 24.720104 12-13 GCAGAGT 22240 0.0 24.554295 9 CATGGGA 9860 0.0 24.198658 4 GTACTTT 12985 0.0 23.415154 14-15 AGTACTT 13000 0.0 23.365232 12-13 GTACCTG 3225 0.0 23.277887 1 >>END_MODULE