FastQCFastQC Report
Thu 26 May 2016
SRR1364619_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364619_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2809705
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50100.046.96571
ATCAACG55150.043.311653
TCAACGC56900.041.9788174
CAACGCA57900.041.3581545
AACGCAG60200.038.7587626
TATCAAC63100.037.382792
GGTATCA38000.033.0765531
ACGCAGA73500.031.4944557
CGCAGAG73050.029.8121978
GTACATG85650.028.167551
TACATGG85100.027.0186482
ACATGGG86150.026.4127713
GCAGAGT88450.022.804859
CAGAGTA83050.021.27476310-11
AGAGTAC76450.019.92096510-11
GTATAGG9450.019.5412031
GAGTACA65200.019.0036541
GTACCTG29500.018.7794271
GTGTAGC18450.018.7263451
TACCTGG28050.018.6876932
Produced by FastQC (version 0.11.5)