FastQCFastQC Report
Thu 26 May 2016
SRR1364619_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364619_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2809705
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56210.20005658957079125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48050.17101439474962674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29440.1047796832763582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51350.052.901781
GTATCAA75800.049.591361
ATCAACG91350.040.449983
TCAACGC91600.040.4081424
CAACGCA94400.038.7494435
TATCAAC95900.038.430672
AACGCAG97700.037.7327236
ACGCAGA120800.030.3195297
CGCAGAG121450.029.9120778
GTACATG91900.027.8739681
TACATGG92150.027.0076582
ACATGGG93400.025.2917523
GCAGAGT139550.024.8379179
GTGGTAT25350.024.6751
TGGTATC25100.024.195382
GAGTACT89250.022.99096312-13
GTACTTT91750.022.10483214-15
CAGAGTA134100.021.95266510-11
AGAGTAC125850.021.68709410-11
CATGGGG51750.020.6983684
Produced by FastQC (version 0.11.5)