##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364619_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2809705 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.531344037897217 31.0 30.0 33.0 26.0 34.0 2 29.593669086256387 31.0 30.0 34.0 26.0 34.0 3 29.619918817100015 31.0 30.0 34.0 25.0 34.0 4 33.274333426462924 35.0 33.0 37.0 28.0 37.0 5 32.682290133661716 35.0 33.0 37.0 26.0 37.0 6 32.692345281800044 35.0 33.0 37.0 25.0 37.0 7 32.46828973148427 35.0 33.0 37.0 24.0 37.0 8 32.38752288941366 35.0 33.0 37.0 23.0 37.0 9 33.66955641250594 37.0 33.0 39.0 23.0 39.0 10-11 33.44870137612312 37.0 33.0 39.0 20.5 39.0 12-13 33.30812113727242 37.0 33.0 39.0 18.5 39.0 14-15 34.27818596614236 38.0 33.0 41.0 19.0 41.0 16-17 34.185514671469065 38.0 33.0 41.0 18.5 41.0 18-19 34.21079882051674 38.5 33.0 41.0 18.0 41.0 20-21 34.2264477943414 39.0 33.0 41.0 18.0 41.0 22-23 34.218477384636465 39.0 33.0 41.0 18.0 41.0 24-25 34.219719152010626 39.0 33.0 41.0 18.5 41.0 26-27 34.12251090416966 38.5 33.0 41.0 18.0 41.0 28-29 33.89258391895235 38.0 33.0 41.0 17.0 41.0 30-31 33.840800902585855 38.0 33.0 40.0 17.0 41.0 32-33 33.67908303540763 38.0 33.0 40.0 16.0 41.0 34-35 33.558958858670216 38.0 32.0 40.0 16.0 41.0 36-37 33.40362546957776 38.0 32.0 40.0 15.0 41.0 38-39 33.27744958990357 38.0 32.0 40.0 13.0 41.0 40-41 33.1148229084548 38.0 32.0 40.0 9.0 41.0 42-43 32.955412578900635 38.0 32.0 40.0 9.0 41.0 44-45 32.776637404994474 38.0 31.0 40.0 8.0 41.0 46-47 32.56223286786335 38.0 31.0 40.0 4.0 41.0 48-49 32.33582564717648 38.0 31.0 40.0 2.0 41.0 50-51 32.144555923130724 38.0 30.5 40.0 2.0 41.0 52-53 31.985883927316216 37.5 30.0 40.0 2.0 41.0 54-55 31.771770345997176 37.0 30.0 40.0 2.0 41.0 56-57 31.54877647297492 37.0 30.0 40.0 2.0 41.0 58-59 31.36027785835168 36.5 30.0 40.0 2.0 41.0 60-61 31.14488620691496 36.0 29.0 40.0 2.0 41.0 62-63 30.874477569709278 36.0 29.0 39.5 2.0 41.0 64-65 30.607351483518734 35.5 29.0 39.0 2.0 41.0 66-67 30.329907766117795 35.0 29.0 39.0 2.0 41.0 68-69 30.012056781761785 35.0 28.0 38.5 2.0 40.0 70-71 29.756101975118384 35.0 28.0 38.0 2.0 40.0 72-73 29.60174253168927 35.0 28.0 37.0 2.0 40.0 74-75 29.320797023175032 35.0 28.0 37.0 2.0 39.0 76-77 29.024665934679973 34.0 28.0 36.5 2.0 39.0 78-79 28.717419622344693 34.0 28.0 36.0 2.0 39.0 80-81 28.443425911261144 34.0 28.0 35.5 2.0 37.5 82-83 28.186005648279803 34.0 27.0 35.0 2.0 37.0 84-85 28.170796578288467 34.0 28.0 35.0 2.0 37.0 86-87 28.025197841054485 34.0 28.0 35.0 2.0 36.0 88-89 27.879100474960893 34.0 27.5 35.0 2.0 36.0 90-91 27.726000594368447 34.0 27.0 35.0 2.0 36.0 92-93 27.59204952121308 34.0 27.0 35.0 2.0 35.0 94-95 27.503958066772135 34.0 27.0 35.0 2.0 35.0 96-97 27.41880891410308 34.0 27.0 35.0 2.0 35.0 98-99 27.331504197059836 34.0 27.0 35.0 2.0 35.0 100-101 27.23169407464485 34.0 26.0 35.0 2.0 35.0 102-103 26.05052576693995 31.5 24.5 34.0 2.0 35.0 104-105 26.984590375146148 33.5 25.5 35.0 2.0 35.0 106-107 27.158670572177506 34.0 26.0 35.0 2.0 35.0 108-109 27.171557868174773 34.0 26.5 35.0 2.0 35.0 110-111 27.110887975783935 34.0 26.0 35.0 2.0 35.0 112-113 27.066026860471116 34.0 26.0 35.0 2.0 35.0 114-115 26.987405617315694 34.0 26.0 35.0 2.0 35.0 116-117 26.876740440722422 34.0 25.0 35.0 2.0 35.0 118-119 26.76832033967979 34.0 25.0 35.0 2.0 35.0 120-121 26.63487572538754 34.0 25.0 35.0 2.0 35.0 122-123 26.455018231451344 34.0 25.0 35.0 2.0 35.0 124-125 25.64469579546607 33.0 22.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7255737307905221 1101 2 0.758071000184529 1101 3 0.6197955943718974 1101 4 0.6707625559033019 1101 5 0.9965902775748035 1101 6 0.9156968058379036 1101 7 0.9302604136682149 1101 8 1.0758343052430348 1101 9 1.0836705093419923 1101 10-11 1.1284057477745364 1101 12-13 1.0936282290925021 1101 14-15 1.1955717732528086 1101 16-17 1.287709330170209 1101 18-19 1.2592669953358495 1101 20-21 1.279453665984228 1101 22-23 1.138657144913573 1101 24-25 1.0167283972154308 1101 26-27 1.1234798148785998 1101 28-29 1.2679151611161856 1101 30-31 1.2759009092267704 1101 32-33 1.3708587766900848 1101 34-35 1.3015902232601277 1101 36-37 1.2706518103381157 1101 38-39 1.3471647752842415 1101 40-41 1.3785844383854595 1101 42-43 1.4010179307868285 1101 44-45 1.262691265709016 1101 46-47 1.2600838061911404 1101 48-49 1.3985802552243172 1101 50-51 1.3808158287477923 1101 52-53 1.3110489642700784 1101 54-55 1.2129889402186613 1101 56-57 1.227674833860128 1101 58-59 1.2663763241380117 1101 60-61 1.249535758633698 1101 62-63 1.2718832869031331 1101 64-65 1.296242143593954 1101 66-67 1.190511120968047 1101 68-69 1.2687939257175742 1101 70-71 1.2283507619706455 1101 72-73 1.237925966407225 1101 74-75 1.1373947579475328 1101 76-77 1.1208516931958314 1101 78-79 1.112436157796587 1101 80-81 1.0974403557256593 1101 82-83 1.0824686867682303 1101 84-85 1.1969857760729532 1101 86-87 1.2030731151793077 1101 88-89 1.138157207895464 1101 90-91 1.1209720716526697 1101 92-93 1.1068227000517012 1101 94-95 1.1285206122796474 1101 96-97 1.2144267590572824 1101 98-99 1.196430748607753 1101 100-101 1.2884856182150486 1101 102-103 1.2499673082080491 1101 104-105 1.1888289009396864 1101 106-107 1.2179449451789708 1101 108-109 1.1881598451586868 1101 110-111 1.190809324583455 1101 112-113 1.1724459487207888 1101 114-115 1.1072492274166095 1101 116-117 1.1064149162431747 1101 118-119 1.1871004585384561 1101 120-121 1.1217279698513885 1101 122-123 1.274989300309553 1101 124-125 1.0890180399330518 1102 1 0.3046170292322259 1102 2 -0.5422603087006266 1102 3 -0.04579202980136898 1102 4 0.26220929398630943 1102 5 0.5318237607492762 1102 6 0.5366243189086077 1102 7 0.424403682679916 1102 8 0.4906074866582273 1102 9 0.644159164646311 1102 10-11 0.775621128106394 1102 12-13 0.8635754494525827 1102 14-15 0.9099293004841797 1102 16-17 1.0416366078239179 1102 18-19 0.9563666567700935 1102 20-21 0.9094018650441953 1102 22-23 0.9238043161891554 1102 24-25 0.8812837805305378 1102 26-27 0.9909160375156887 1102 28-29 0.9491702925339638 1102 30-31 1.008136011338749 1102 32-33 0.9077080059610481 1102 34-35 0.7932876913715958 1102 36-37 0.6970736479270698 1102 38-39 0.6904498245567794 1102 40-41 0.7438400636384017 1102 42-43 0.5544584346321031 1102 44-45 0.39412295412501663 1102 46-47 0.5008823597394283 1102 48-49 0.5504832744616834 1102 50-51 0.3434904464596755 1102 52-53 0.5006214058295626 1102 54-55 0.48509200295125154 1102 56-57 0.4221441962411845 1102 58-59 0.634170042494862 1102 60-61 0.4185911545419536 1102 62-63 0.5936737982065381 1102 64-65 0.469084337496362 1102 66-67 0.3844193122490154 1102 68-69 0.2440263209365945 1102 70-71 0.1389997181946967 1102 72-73 0.16667284874208477 1102 74-75 0.37209808710269954 1102 76-77 0.4274449274700167 1102 78-79 0.44850122535275716 1102 80-81 0.44321482187132943 1102 82-83 0.35184360921115143 1102 84-85 0.4125594615358317 1102 86-87 0.37118296164763365 1102 88-89 0.45968628212473206 1102 90-91 0.44647635222832704 1102 92-93 0.584210017596341 1102 94-95 0.38277148767550884 1102 96-97 0.34826301877225774 1102 98-99 0.4153302598213422 1102 100-101 0.21426097910631725 1102 102-103 0.17430247945005561 1102 104-105 0.21262211856102198 1102 106-107 0.4996192844616729 1102 108-109 0.5952641194780313 1102 110-111 0.5333167042220595 1102 112-113 0.5612878672991677 1102 114-115 0.711125971494873 1102 116-117 0.7120082665582714 1102 118-119 0.6398327045373762 1102 120-121 0.5520702422452999 1102 122-123 0.5047883770194979 1102 124-125 0.3851487022322537 1103 1 0.35533454735714187 1103 2 -0.8964644190412585 1103 3 0.08150338726145279 1103 4 0.35986785788551146 1103 5 0.5967812351951878 1103 6 0.6483844827560219 1103 7 0.5184675957700335 1103 8 0.6854859532814501 1103 9 0.7080360531585015 1103 10-11 0.8735693338956096 1103 12-13 0.7855457964452484 1103 14-15 0.8738467894153814 1103 16-17 1.0315647171320705 1103 18-19 1.029425881781492 1103 20-21 1.0074249436542004 1103 22-23 1.0153306885640845 1103 24-25 0.8725056759117393 1103 26-27 0.907831437678233 1103 28-29 1.0287229406493665 1103 30-31 0.93358392048399 1103 32-33 0.8287772932752304 1103 34-35 0.9679558423621515 1103 36-37 0.996840045888348 1103 38-39 0.9296149400166485 1103 40-41 0.9496012962176081 1103 42-43 0.9079995849319928 1103 44-45 1.0127613356587801 1103 46-47 0.8590624289606055 1103 48-49 0.9317586598925587 1103 50-51 1.014893770568868 1103 52-53 0.8764850000434024 1103 54-55 0.9256978376736704 1103 56-57 0.9576470167703661 1103 58-59 0.9689746834127142 1103 60-61 0.874086511613541 1103 62-63 0.9008994118353577 1103 64-65 0.937898921478336 1103 66-67 1.041263431859118 1103 68-69 0.8215592623628609 1103 70-71 0.9436076885212366 1103 72-73 0.8691295663245917 1103 74-75 0.7432815634585239 1103 76-77 0.6215667921815431 1103 78-79 0.6689540917353654 1103 80-81 0.7444070865588657 1103 82-83 0.6970620803738186 1103 84-85 0.7201334066541989 1103 86-87 0.6592247465505991 1103 88-89 0.684321683156579 1103 90-91 0.7537737879809399 1103 92-93 0.8219933997466384 1103 94-95 0.762048085482423 1103 96-97 0.7441977006182618 1103 98-99 0.724397332271387 1103 100-101 0.7660479844387034 1103 102-103 0.8011096978291725 1103 104-105 0.7822207491060418 1103 106-107 0.7592931318298994 1103 108-109 0.6934161118272328 1103 110-111 0.799680962558103 1103 112-113 0.7519845256295916 1103 114-115 0.6646760298300052 1103 116-117 0.7472655495598701 1103 118-119 0.8083629693692522 1103 120-121 0.7842447872062408 1103 122-123 0.8684512751165734 1103 124-125 0.8889235475444792 1104 1 0.48024082706185567 1104 2 -0.5598338958184748 1104 3 0.22177797004356847 1104 4 0.48160042499790734 1104 5 0.8841213859051749 1104 6 0.7820110280856127 1104 7 0.8863970469193418 1104 8 0.8228511569151706 1104 9 0.9187167671075045 1104 10-11 1.0750791490287597 1104 12-13 1.0051707236023901 1104 14-15 1.0480755563780093 1104 16-17 1.2096833041654236 1104 18-19 1.1611585127811281 1104 20-21 1.1623348945933714 1104 22-23 1.1161589523690836 1104 24-25 1.133378313966361 1104 26-27 1.021074645894494 1104 28-29 1.3648691187551236 1104 30-31 1.2505022077949377 1104 32-33 1.3296494681473803 1104 34-35 1.3482524365791662 1104 36-37 1.3856497285467455 1104 38-39 1.4078253421366185 1104 40-41 1.3479893869977246 1104 42-43 1.3243616083503085 1104 44-45 1.3453544707013378 1104 46-47 1.4704727579753225 1104 48-49 1.4460015208360488 1104 50-51 1.3268825300658236 1104 52-53 1.1579062834375975 1104 54-55 1.373337227075968 1104 56-57 1.5058153696798087 1104 58-59 1.420575423774526 1104 60-61 1.339278691268106 1104 62-63 1.3919151550196176 1104 64-65 1.3534009770501925 1104 66-67 1.301463770826544 1104 68-69 1.3631846495081135 1104 70-71 1.4170094922404814 1104 72-73 1.302860019682118 1104 74-75 1.3079266059686319 1104 76-77 1.309395326594629 1104 78-79 1.3836816628658184 1104 80-81 1.196033724068613 1104 82-83 1.2821721443933924 1104 84-85 1.2785108259840143 1104 86-87 1.2168461859209287 1104 88-89 1.141784876122049 1104 90-91 1.3016852229293576 1104 92-93 1.2869973402201609 1104 94-95 1.2298011736317527 1104 96-97 1.18367754898647 1104 98-99 1.3112530580053026 1104 100-101 1.3895214787648342 1104 102-103 1.3556117287588094 1104 104-105 1.291437281662752 1104 106-107 1.3037169510910793 1104 108-109 1.2572569166968997 1104 110-111 1.1698126409990337 1104 112-113 1.1791412674900137 1104 114-115 1.2648081509182276 1104 116-117 1.3026695884197395 1104 118-119 1.1635526209145688 1104 120-121 1.199203687177537 1104 122-123 1.226179759834519 1104 124-125 1.088422968224144 1105 1 0.5512991281541986 1105 2 0.29590659364268035 1105 3 0.465066002241187 1105 4 0.4434420958468337 1105 5 0.6714266675856564 1105 6 0.5403246450688499 1105 7 0.777470961767726 1105 8 0.7108647224721452 1105 9 0.7469315943338586 1105 10-11 0.9375017330345727 1105 12-13 0.8572470920410069 1105 14-15 0.8764686273754592 1105 16-17 0.9653587805664401 1105 18-19 0.8813746535456843 1105 20-21 0.9925724732928103 1105 22-23 0.8469334088307008 1105 24-25 0.9712600167509038 1105 26-27 0.9513538922563711 1105 28-29 1.0910490681785916 1105 30-31 1.0193234665787827 1105 32-33 0.9313868304316557 1105 34-35 0.9227838911279989 1105 36-37 0.9633997500112343 1105 38-39 0.9569182793903792 1105 40-41 0.8126820730505884 1105 42-43 0.8514401674944949 1105 44-45 0.894775460581613 1105 46-47 0.8370504908473251 1105 48-49 0.861143213016689 1105 50-51 0.9440001944107834 1105 52-53 0.8019554641137177 1105 54-55 0.815609811922652 1105 56-57 0.8328130276164103 1105 58-59 0.7695933238305521 1105 60-61 0.8755957213263628 1105 62-63 0.8410013873866475 1105 64-65 0.8301673274586356 1105 66-67 0.8249485770663831 1105 68-69 0.6684309246448024 1105 70-71 0.6882586723855155 1105 72-73 0.7310439336188104 1105 74-75 0.6730579199582571 1105 76-77 0.7747147978512459 1105 78-79 0.7995070100815873 1105 80-81 0.6004668491300222 1105 82-83 0.6009210213801843 1105 84-85 0.4113115080679357 1105 86-87 0.5505295895781224 1105 88-89 0.6704846680271466 1105 90-91 0.6209682780209569 1105 92-93 0.4820360780905091 1105 94-95 0.5852317557044344 1105 96-97 0.6570918985947536 1105 98-99 0.7208904364875295 1105 100-101 0.7828039124926711 1105 102-103 0.39649770762358827 1105 104-105 0.28109150866233534 1105 106-107 0.6519644530228845 1105 108-109 0.7042471527885752 1105 110-111 0.6112773201868009 1105 112-113 0.5021452027938302 1105 114-115 0.6016417449233948 1105 116-117 0.2602103952897892 1105 118-119 0.10740417339458119 1105 120-121 0.14425691910996719 1105 122-123 0.22659289592468923 1105 124-125 0.05280397662140501 1106 1 0.32672250030038796 1106 2 0.33391038772530734 1106 3 0.5147605000423958 1106 4 0.3889112462592479 1106 5 0.502769968446195 1106 6 0.5869608210213286 1106 7 0.5268444750722239 1106 8 0.5989927319722881 1106 9 0.5687113575098763 1106 10-11 0.6532370599599062 1106 12-13 0.5632746340861061 1106 14-15 0.5785934348204691 1106 16-17 0.7246830242437454 1106 18-19 0.7253432219924818 1106 20-21 0.8048529378785929 1106 22-23 0.7153361214677787 1106 24-25 0.7245699394649137 1106 26-27 0.7208773566472004 1106 28-29 0.8158554150335604 1106 30-31 0.8233036451261384 1106 32-33 0.6686429030952397 1106 34-35 0.6677068658843908 1106 36-37 0.6842262212568926 1106 38-39 0.762598778857317 1106 40-41 0.8602141612220038 1106 42-43 0.8075337608525288 1106 44-45 0.7361017871743485 1106 46-47 0.6267801932950832 1106 48-49 0.7633276289315916 1106 50-51 0.8397189387239266 1106 52-53 0.8846913062501045 1106 54-55 0.8418572566943432 1106 56-57 0.7320770666850223 1106 58-59 0.7285436499974587 1106 60-61 0.8717617698912221 1106 62-63 0.8558909004721684 1106 64-65 0.7167120529069706 1106 66-67 0.6577730556288621 1106 68-69 0.7334302852291401 1106 70-71 0.6515690343596958 1106 72-73 0.7904108915604091 1106 74-75 0.7782749296265266 1106 76-77 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2216 104-105 -1.8196305454317532 2216 106-107 -2.0236370230995746 2216 108-109 -2.0551766875985393 2216 110-111 -1.927844242231302 2216 112-113 -2.007239265814853 2216 114-115 -2.0388188988036617 2216 116-117 -2.1322420195835576 2216 118-119 -2.2952966089941 2216 120-121 -2.2936552304432 2216 122-123 -2.2245830585293085 2216 124-125 -2.3122682546304816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 115194.0 3 35794.0 4 16421.0 5 12663.0 6 13433.0 7 15784.0 8 17540.0 9 19066.0 10 20652.0 11 22168.0 12 23768.0 13 25724.0 14 27242.0 15 22621.0 16 16454.0 17 14554.0 18 13397.0 19 12626.0 20 12286.0 21 13035.0 22 15360.0 23 20533.0 24 28064.0 25 37449.0 26 44423.0 27 50768.0 28 58393.0 29 68240.0 30 79084.0 31 93640.0 32 113217.0 33 138383.0 34 177652.0 35 239487.0 36 376934.0 37 573693.0 38 223071.0 39 892.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.76947419478219 19.43516341535856 13.609090889665875 25.186271500193374 2 15.775037371603565 20.353909174804365 39.143713895041806 24.72733955855027 3 19.61544005509475 24.321734844049463 29.011337489167012 27.051487611688774 4 13.179201244351743 17.222774911505446 36.2201392065122 33.37788463763061 5 13.435777520334206 38.18394525233782 34.234199914981915 14.146077312346064 6 32.44428910866845 37.35697679881439 16.66785303970126 13.530881052815905 7 28.42714280731551 32.38008870691625 20.479858718113167 18.712909767655077 8 25.74491832591618 37.077713524271594 18.839457821891557 18.337910327920664 9 25.692096171497987 16.717544183715706 19.466923207515137 38.12343643727117 10-11 24.884889041386884 27.2215270980607 27.051231076068227 20.842352784484188 12-13 25.551890130261018 24.037582632243627 27.932189948445167 22.478337289050188 14-15 22.41664763898675 25.66106431260897 25.627375086024983 26.294912962379296 16-17 22.21281129577062 28.934836050760605 26.27957787217192 22.572774781296857 18-19 22.188673779047345 27.5276572974033 28.450299791632673 21.833369131916683 20-21 22.70384096045635 26.720976915296763 28.77688571764686 21.798296406600024 22-23 22.35604133058631 26.398367165619923 28.750496556496387 22.495094947297382 24-25 22.34747064193572 26.58962417762719 28.795371756109628 22.267533424327464 26-27 22.15892771661082 26.882946786228445 28.642740074135897 22.315385423024836 28-29 22.094437901217752 26.936657114445932 28.665066024273145 22.30383896006317 30-31 22.275826109858507 26.719815781372063 28.7312547046754 22.273103404094023 32-33 22.19920003075162 26.682992787605958 28.67732794041019 22.44047924123223 34-35 22.460365056117993 26.757702321062176 28.643683233649085 22.13824938917075 36-37 22.494289429982253 26.5707893577326 28.564470538156716 22.37045067412843 38-39 22.207438147421172 26.888000697582132 28.541394915124542 22.363166239872157 40-41 22.500315869459605 26.672479851087573 28.50825976392539 22.31894451552743 42-43 22.328006133244305 26.59323050990068 28.421867692480724 22.656895664374293 44-45 22.411836829845125 26.910867866911296 28.253660081752358 22.423635221491224 46-47 22.653724850326736 27.037230084953805 27.832136024317155 22.476909040402308 48-49 22.516796707124687 27.124508733575887 27.799580204665798 22.559114354633632 50-51 22.647442736171307 27.27631226548813 27.589068910565835 22.487176087774728 52-53 22.690476537911657 27.22084860926582 27.48742482746397 22.601250025358553 54-55 22.652609437645587 27.173991575628047 27.538976511769032 22.634422474957336 56-57 22.534399679960764 27.41200834052431 27.521238813797634 22.532353165717293 58-59 22.607492214036107 27.0800815815619 27.625032321026044 22.68739388337595 60-61 22.636504544071354 27.282508306032128 27.616226614537826 22.464760535358693 62-63 22.523805879976724 27.18196394283386 27.76396454432049 22.530265632868932 64-65 22.47664292327867 27.112075707875 27.640442759880123 22.77083860896621 66-67 22.446057504257563 27.293167788077394 27.52897546183674 22.7317992458283 68-69 22.52117759568579 27.063331875284508 27.692137676349464 22.723352852680243 70-71 22.47721735911777 26.918911415967155 27.661427089320767 22.942444135594307 72-73 22.58201545189515 27.073098419254116 27.49788850674984 22.8469976221009 74-75 22.46195845127007 27.337451961449926 27.38806428334358 22.812525303936425 76-77 22.589877640913684 27.207448105318715 27.46623193166769 22.73644232209991 78-79 22.613665281571713 27.23019566319948 27.46431526778008 22.691823787448726 80-81 22.57959578810997 27.190323401544315 27.43234513723166 22.797735673114055 82-83 22.65366576241893 27.256751783641114 27.380341402753743 22.70924105118621 84-85 22.555790614093592 27.2536197788947 27.439831697454036 22.750757909557667 86-87 22.618915847509534 27.182397247817114 27.49869469910812 22.699992205565227 88-89 22.58817206788613 27.21202047901826 27.41709538901771 22.782712064077902 90-91 22.531066428682013 27.379440190340265 27.435495897256118 22.6539974837216 92-93 22.544081142475576 27.197454473749218 27.538336303042772 22.720128080732437 94-95 22.504271613581675 27.155841241372464 27.49730337986475 22.84258376518111 96-97 22.597726637705353 27.129679889090387 27.45386626422186 22.8187272089824 98-99 22.422320600596475 27.169046510208396 27.33917303914494 23.06945985005019 100-101 22.4218192081367 27.095858812868823 27.511847839414283 22.97047413958019 102-103 22.30094549189656 26.842442159781033 27.65756433557088 23.199048012751533 104-105 22.339459271692412 26.915120945641018 27.55796720779359 23.187452574872975 106-107 22.328490401605805 27.02896202374857 27.38926774438048 23.253279830265146 108-109 22.36945934315634 26.978028685967427 27.302460520934662 23.350051449941574 110-111 22.320972398374685 26.975840414083997 27.394323500096007 23.30886368744531 112-113 22.358392888349783 26.90920409298591 27.502494138818268 23.229908879846043 114-115 22.189187741496795 27.062785029169582 27.348830769794752 23.39919645953887 116-117 22.055939018326605 27.073786557736586 27.467930842163973 23.40234358177284 118-119 22.060703884596595 27.049221025244496 27.35896014500169 23.53111494515722 120-121 21.974422079822077 27.049604563132856 27.427759532808555 23.548213824236516 122-123 22.002139393726925 27.079987379320986 27.35943256060444 23.55844066634765 124-125 22.00883668469856 26.991466199894575 27.34350280515267 23.656194310254193 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 73.0 1 69.5 2 117.0 3 215.5 4 210.5 5 220.5 6 528.5 7 1044.5 8 1581.5 9 1874.5 10 1666.0 11 1394.0 12 1421.0 13 1565.5 14 1745.5 15 1972.0 16 2201.5 17 2474.5 18 2811.5 19 3129.5 20 3625.5 21 4310.5 22 5186.5 23 6641.5 24 8602.0 25 10913.0 26 14043.0 27 17818.0 28 21944.0 29 26097.5 30 30881.0 31 37079.0 32 43555.5 33 49188.5 34 54532.5 35 60617.0 36 67918.0 37 73964.0 38 77754.5 39 81834.5 40 86070.5 41 90851.5 42 94871.5 43 97615.0 44 100818.5 45 104430.5 46 107198.5 47 108536.5 48 108742.0 49 107931.5 50 106046.0 51 103073.0 52 100844.5 53 98539.5 54 95628.0 55 89143.5 56 80968.5 57 74011.5 58 67285.5 59 59138.0 60 48479.5 61 39110.5 62 31012.5 63 23241.5 64 16615.5 65 12002.0 66 8837.5 67 6672.5 68 5091.5 69 3900.0 70 2934.0 71 2008.0 72 1481.0 73 1168.5 74 872.0 75 641.0 76 425.0 77 232.5 78 153.0 79 101.5 80 54.0 81 41.5 82 31.0 83 15.0 84 12.5 85 7.0 86 7.5 87 11.5 88 6.0 89 3.0 90 3.0 91 1.5 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06075370901927426 2 0.07445621515425997 3 0.0 4 0.007474094255446747 5 0.03171151419810976 6 7.474094255446747E-4 7 1.0677277507781065E-4 8 3.559092502593689E-5 9 5.694548004149902E-4 10-11 0.05884959453038664 12-13 0.009573958831977022 14-15 0.007171571392726282 16-17 0.0535109557764961 18-19 1.2456823759077909E-4 20-21 0.010339163720034666 22-23 0.01270596023425947 24-25 0.0 26-27 0.0 28-29 0.05187377322530302 30-31 0.0 32-33 0.003452319727515878 34-35 0.0 36-37 1.0677277507781065E-4 38-39 0.0 40-41 0.0 42-43 0.0035235015775677515 44-45 0.0 46-47 5.3386387538905325E-5 48-49 8.897731256484223E-5 50-51 4.448865628242111E-4 52-53 7.118185005187378E-5 54-55 0.0 56-57 0.0017617507887838758 58-59 5.3386387538905325E-5 60-61 0.0 62-63 0.0 64-65 3.7370471277233734E-4 66-67 0.0 68-69 4.982729503631163E-4 70-71 0.0 72-73 0.0029184558521268248 74-75 0.0040039790654179 76-77 8.541822006224852E-4 78-79 0.0010677277507781064 80-81 0.0014236370010374756 82-83 1.0677277507781065E-4 84-85 1.0677277507781065E-4 86-87 2.847274002074951E-4 88-89 0.0 90-91 0.0 92-93 0.008079139980887672 94-95 0.006032661791896303 96-97 0.007474094255446747 98-99 0.001121114138317012 100-101 5.516593379020217E-4 102-103 1.2456823759077909E-4 104-105 4.6268202533717947E-4 106-107 0.005214070516299754 108-109 0.006335184654616766 110-111 0.0011922959883688858 112-113 0.004199729153060553 114-115 5.160684128760848E-4 116-117 0.0026337284519193298 118-119 0.002936251314639793 120-121 0.014485506485556313 122-123 0.001085523213291075 124-125 0.0019219099514005918 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2809705.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.8456443896406 #Duplication Level Percentage of deduplicated Percentage of total 1 78.83824522517267 47.96963833274137 2 11.902353133432943 14.484126923135705 3 4.04431996754265 7.382377636290687 4 1.7668449001205815 4.30019265937548 5 0.9639745112197187 2.9326825155176306 6 0.579751360243864 2.116520705988514 7 0.37123123070736463 1.5811462414964255 8 0.27084462286540506 1.3183772486171805 9 0.1903859404184886 1.0425739704741592 >10 0.9836934594011578 10.794571425018928 >50 0.0624636732434156 2.580341528946151 >100 0.024776846420218643 2.6053919328144035 >500 8.216741559147003E-4 0.3170415826014677 >1k 2.3476404452343498E-4 0.37428619361984433 >5k 5.8691011130858745E-5 0.20073110336200217 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5621 0.20005658957079125 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4805 0.17101439474962674 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2944 0.1047796832763582 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.559092502593689E-5 0.0 0.0 0.0 0.0 3 3.559092502593689E-5 0.0 0.0 0.0 0.0 4 3.559092502593689E-5 0.0 0.0 0.0 0.0 5 7.118185005187378E-5 0.0 0.0 0.0 0.0 6 7.118185005187378E-5 0.0 0.0 0.0 0.0 7 1.4236370010374755E-4 0.0 0.0 0.0 0.0 8 1.4236370010374755E-4 0.0 0.0 0.0 0.0 9 1.4236370010374755E-4 0.0 0.0 3.559092502593689E-5 0.0 10-11 1.4236370010374755E-4 0.0 0.0 3.559092502593689E-5 0.0 12-13 1.7795462512968442E-4 0.0 0.0 3.559092502593689E-5 0.0 14-15 1.9575008764265286E-4 0.0 0.0 5.338638753890533E-5 0.0 16-17 2.1354555015562133E-4 0.0 0.0 7.118185005187378E-5 0.0 18-19 2.313410126685898E-4 0.0 0.0 8.897731256484221E-5 0.0 20-21 2.491364751815582E-4 0.0 0.0 1.245682375907791E-4 0.0 22-23 2.847274002074951E-4 0.0 0.0 2.1354555015562133E-4 0.0 24-25 3.5590925025936885E-4 0.0 0.0 3.0252286272046354E-4 0.0 26-27 3.5590925025936885E-4 0.0 0.0 4.8047748785014796E-4 0.0 28-29 3.915001752853058E-4 0.0 0.0 8.363867381095168E-4 0.0 30-31 3.915001752853058E-4 0.0 0.0 0.00217104642658215 0.0 32-33 3.915001752853058E-4 0.0 0.0 0.004555638403319922 0.0 34-35 4.8047748785014796E-4 0.0 0.0 0.00754527610549862 0.0 36-37 4.982729503631165E-4 0.0 0.0 0.012972892171953996 0.0 38-39 4.982729503631165E-4 0.0 0.0 0.02322307857942382 0.0 40-41 4.982729503631165E-4 0.0 0.0 0.03587565242614438 0.0 42-43 5.160684128760848E-4 0.0 0.0 0.04808333971004074 0.0 44-45 5.516593379020218E-4 0.0 0.0 0.062266323332876586 0.0 46-47 6.584321129798323E-4 0.0 0.0 0.07762380748156836 3.559092502593689E-5 48-49 6.762275754928008E-4 0.0 0.0 0.09680731607054834 3.559092502593689E-5 50-51 6.762275754928008E-4 0.0 0.0 0.11782375729836406 3.559092502593689E-5 52-53 6.762275754928008E-4 0.0 0.0 0.1383241301133037 7.118185005187378E-5 54-55 6.762275754928008E-4 0.0 0.0 0.16035491270435864 1.0677277507781066E-4 56-57 7.474094255446746E-4 0.0 0.0 0.18843615254982282 1.0677277507781066E-4 58-59 7.474094255446746E-4 0.0 0.0 0.2192578936222842 1.0677277507781066E-4 60-61 7.474094255446746E-4 0.0 0.0 0.25424377292278016 1.0677277507781066E-4 62-63 7.474094255446746E-4 0.0 0.0 0.2914362895748842 1.7795462512968442E-4 64-65 7.474094255446746E-4 0.0 0.0 0.3288779427021698 1.7795462512968442E-4 66-67 7.830003505706116E-4 0.0 0.0 0.3727081668716111 1.9575008764265286E-4 68-69 7.830003505706116E-4 0.0 0.0 0.42504462212225125 2.1354555015562133E-4 70-71 8.0079581308358E-4 0.0 0.0 0.4867948770422518 2.491364751815582E-4 72-73 8.185912755965484E-4 0.0 0.0 0.5497018370255953 2.491364751815582E-4 74-75 8.185912755965484E-4 0.0 0.0 0.6133917973595093 2.491364751815582E-4 76-77 8.185912755965484E-4 0.0 0.0 0.6876166715011007 2.491364751815582E-4 78-79 8.185912755965484E-4 0.0 0.0 0.7723230730628304 2.491364751815582E-4 80-81 9.25364050674359E-4 0.0 0.0 0.8725293224733558 2.847274002074951E-4 82-83 9.25364050674359E-4 0.0 0.0 0.9768107327993508 3.20318325233432E-4 84-85 9.25364050674359E-4 0.0 0.0 1.0852740768158935 3.381137877464004E-4 86-87 9.431595131873275E-4 0.0 0.0 1.2010513559252662 3.5590925025936885E-4 88-89 9.609549757002959E-4 0.0 0.0 1.3312073687451174 3.5590925025936885E-4 90-91 9.96545900726233E-4 0.0 3.559092502593689E-5 1.4842305508941331 3.5590925025936885E-4 92-93 9.96545900726233E-4 0.0 3.559092502593689E-5 1.6513833302784455 3.5590925025936885E-4 94-95 9.96545900726233E-4 0.0 3.559092502593689E-5 1.821917247540222 3.5590925025936885E-4 96-97 9.96545900726233E-4 0.0 3.559092502593689E-5 2.0012421232834052 3.5590925025936885E-4 98-99 0.001085523213291075 0.0 3.559092502593689E-5 2.19412714146147 3.5590925025936885E-4 100-101 0.0011389096008299804 0.0 3.559092502593689E-5 2.421375197752077 3.5590925025936885E-4 102-103 0.0011922959883688858 0.0 3.559092502593689E-5 2.6682516491944885 3.7370471277233734E-4 104-105 0.0013346596884726333 0.0 5.338638753890533E-5 2.918687193139493 3.915001752853058E-4 106-107 0.001405841538524507 0.0 7.118185005187378E-5 3.174461375838389 3.915001752853058E-4 108-109 0.0014236370010374754 0.0 7.118185005187378E-5 3.4522485456658263 4.092956377982742E-4 110-111 0.0014592279260634122 0.0 7.118185005187378E-5 3.766818936507569 4.2709110031124265E-4 112-113 0.0014948188510893491 0.0 7.118185005187378E-5 4.1114992499212555 4.2709110031124265E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5135 0.0 52.90178 1 GTATCAA 7580 0.0 49.59136 1 ATCAACG 9135 0.0 40.44998 3 TCAACGC 9160 0.0 40.408142 4 CAACGCA 9440 0.0 38.749443 5 TATCAAC 9590 0.0 38.43067 2 AACGCAG 9770 0.0 37.732723 6 ACGCAGA 12080 0.0 30.319529 7 CGCAGAG 12145 0.0 29.912077 8 GTACATG 9190 0.0 27.873968 1 TACATGG 9215 0.0 27.007658 2 ACATGGG 9340 0.0 25.291752 3 GCAGAGT 13955 0.0 24.837917 9 GTGGTAT 2535 0.0 24.675 1 TGGTATC 2510 0.0 24.19538 2 GAGTACT 8925 0.0 22.990963 12-13 GTACTTT 9175 0.0 22.104832 14-15 CAGAGTA 13410 0.0 21.952665 10-11 AGAGTAC 12585 0.0 21.687094 10-11 CATGGGG 5175 0.0 20.698368 4 >>END_MODULE