FastQCFastQC Report
Thu 26 May 2016
SRR1364618_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364618_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2957718
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32130.1086310459617854No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74750.039.530431
ATCAACG80000.035.2216843
GTACATG122750.034.895421
CAACGCA82500.034.0099455
TCAACGC83400.033.5715034
TACATGG124400.033.233652
AACGCAG86050.031.8869886
TATCAAC92800.031.069652
ACATGGG125250.030.439763
GGTATCA51950.027.4077531
ACGCAGA98500.027.0664717
CGCAGAG95800.026.1485488
CATGGGA81800.024.469494
GTATAGG9550.023.0812551
CATGGGG52250.021.7763964
GCAGAGT110600.021.574589
GTGTAGC19500.021.385751
GGACCGA3951.4551915E-1121.0957226
GTACCTG28550.020.8667561
AGTACAT93700.020.0903022
Produced by FastQC (version 0.11.5)