##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364618_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2957718 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.039181896313305 31.0 27.0 31.0 10.0 34.0 2 26.84096692111959 31.0 26.0 31.0 10.0 34.0 3 27.598619273372243 31.0 27.0 31.0 15.0 34.0 4 31.042043223863804 35.0 32.0 37.0 16.0 37.0 5 31.50696144798118 35.0 32.0 37.0 17.0 37.0 6 31.547405465970726 35.0 32.0 37.0 17.0 37.0 7 31.284839866410522 35.0 32.0 37.0 15.0 37.0 8 31.121442950274503 35.0 32.0 37.0 11.0 37.0 9 32.19611234066263 37.0 32.0 39.0 10.0 39.0 10-11 32.17086787854691 37.0 31.5 39.0 10.5 39.0 12-13 32.16527116513474 37.0 32.0 39.0 10.0 39.0 14-15 32.89049530753101 38.0 31.5 40.0 10.0 41.0 16-17 33.32301879354286 38.0 32.0 40.0 10.0 41.0 18-19 33.151109909734465 38.0 32.0 40.0 10.0 41.0 20-21 33.0655750480607 38.0 32.0 40.0 10.0 41.0 22-23 33.13537514394543 38.0 32.0 40.0 10.0 41.0 24-25 33.21834721903846 38.0 32.0 40.0 10.0 41.0 26-27 33.011335259142356 38.0 31.5 40.0 9.5 41.0 28-29 32.929087729120894 38.0 31.0 40.0 9.0 41.0 30-31 32.809894824320644 38.0 31.0 40.0 8.0 41.0 32-33 32.711141325846484 38.0 31.0 40.0 8.0 41.0 34-35 32.79275694978358 38.0 31.0 40.0 8.0 41.0 36-37 32.75348613356648 38.0 31.0 40.0 4.5 41.0 38-39 32.47130355226564 38.0 30.0 40.0 2.0 41.0 40-41 32.347782817699326 38.0 30.0 40.0 2.0 41.0 42-43 31.87609231170788 38.0 30.0 40.0 2.0 41.0 44-45 31.780360230420886 37.0 30.0 40.0 2.0 41.0 46-47 31.77181191716046 37.5 30.0 40.0 2.0 41.0 48-49 31.736708672023497 37.0 30.0 40.0 2.0 41.0 50-51 31.5123837025707 37.0 30.0 40.0 2.0 41.0 52-53 31.42410872165636 37.0 30.0 40.0 2.0 41.0 54-55 31.259136604639117 37.0 29.5 40.0 2.0 41.0 56-57 31.041988959055594 36.5 29.0 40.0 2.0 41.0 58-59 30.83916350375526 36.0 29.0 40.0 2.0 41.0 60-61 30.537161250666898 36.0 28.0 40.0 2.0 41.0 62-63 30.37511520706166 35.5 28.0 39.0 2.0 41.0 64-65 30.153584283559148 35.0 28.0 39.0 2.0 40.5 66-67 29.885752630913426 35.0 28.0 39.0 2.0 40.0 68-69 29.649873314494485 35.0 28.0 38.5 2.0 40.0 70-71 29.197112436006407 34.0 28.0 38.0 2.0 40.0 72-73 28.96297517207523 34.0 27.5 37.0 2.0 39.5 74-75 28.93082690777146 34.0 28.0 37.0 2.0 39.0 76-77 28.663243588469218 34.0 28.0 36.5 2.0 39.0 78-79 28.357968034816032 34.0 27.0 36.0 2.0 39.0 80-81 27.918320475447626 34.0 26.0 35.5 2.0 37.5 82-83 28.015718537061343 34.0 27.5 35.0 2.0 37.0 84-85 27.82865405018328 34.0 27.0 35.0 2.0 37.0 86-87 27.695290423224932 34.0 27.0 35.0 2.0 36.0 88-89 27.524993762082794 34.0 27.0 35.0 2.0 36.0 90-91 27.382615414992234 34.0 27.0 35.0 2.0 36.0 92-93 27.252337105836325 34.0 27.0 35.0 2.0 35.5 94-95 27.140637985095267 34.0 26.0 35.0 2.0 35.0 96-97 27.031505370018373 34.0 26.0 35.0 2.0 35.0 98-99 26.941658062060007 34.0 26.0 35.0 2.0 35.0 100-101 26.838145320142083 34.0 25.5 35.0 2.0 35.0 102-103 25.678916482233937 31.5 24.0 34.0 2.0 35.0 104-105 26.545379917896163 33.0 25.0 35.0 2.0 35.0 106-107 26.707986021655884 34.0 25.0 35.0 2.0 35.0 108-109 26.668190476576875 34.0 25.0 35.0 2.0 35.0 110-111 26.601639000066942 34.0 25.0 35.0 2.0 35.0 112-113 26.513670336387715 34.0 25.0 35.0 2.0 35.0 114-115 26.415634282916763 34.0 25.0 35.0 2.0 35.0 116-117 26.306139733402574 34.0 25.0 35.0 2.0 35.0 118-119 26.185189392633106 34.0 25.0 35.0 2.0 35.0 120-121 26.009502427209085 33.5 24.0 35.0 2.0 35.0 122-123 25.80907054019349 33.0 23.5 35.0 2.0 35.0 124-125 24.853490427417356 32.0 18.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.4214250272141058 1101 2 1.677527654288106 1101 3 1.351390550739584 1101 4 1.4825434915922955 1101 5 1.3681355084488054 1101 6 1.3145186573295256 1101 7 1.3644188734502265 1101 8 1.4812179426944958 1101 9 1.9387558897548374 1101 10-11 1.8979017315602675 1101 12-13 1.9560736027477432 1101 14-15 2.4098961334775026 1101 16-17 1.8693212761333342 1101 18-19 1.959063201720305 1101 20-21 2.1009302147463345 1101 22-23 1.8664433311440192 1101 24-25 1.8661254431012182 1101 26-27 2.037148959132324 1101 28-29 2.014530053654731 1101 30-31 2.16237454192774 1101 32-33 2.1408663427050243 1101 34-35 1.9350420006628752 1101 36-37 1.8224750870197326 1101 38-39 1.9095333872025222 1101 40-41 2.000062874029126 1101 42-43 2.195784882200801 1101 44-45 1.9679154418982954 1101 46-47 1.8089511560934355 1101 48-49 1.710474915062381 1101 50-51 1.7556015940066736 1101 52-53 1.6363179682271642 1101 54-55 1.7224347129592452 1101 56-57 1.7724778123027214 1101 58-59 1.7283189004938748 1101 60-61 1.821962652325169 1101 62-63 1.5833516655591353 1101 64-65 1.5550472945940683 1101 66-67 1.5528581462097755 1101 68-69 1.3665019916529637 1101 70-71 1.3292485535437208 1101 72-73 1.367082047631488 1101 74-75 1.4389210315857568 1101 76-77 1.4243930269250136 1101 78-79 1.3891303105295947 1101 80-81 1.5048298507830005 1101 82-83 1.554402232897182 1101 84-85 1.5139808526178662 1101 86-87 1.4974783516803392 1101 88-89 1.397333506317512 1101 90-91 1.5105793210901801 1101 92-93 1.4512294945656485 1101 94-95 1.3572222738073414 1101 96-97 1.3717889826685408 1101 98-99 1.4665355745179802 1101 100-101 1.4676860380882708 1101 102-103 1.3711819014869633 1101 104-105 1.5460550243682825 1101 106-107 1.5232549638664494 1101 108-109 1.4550914939743471 1101 110-111 1.5546561848454736 1101 112-113 1.4867463342259555 1101 114-115 1.5037851937989757 1101 116-117 1.4524656161903842 1101 118-119 1.3596803464371092 1101 120-121 1.4178303699395443 1101 122-123 1.44424804324985 1101 124-125 1.390147241989208 1102 1 0.8061423004465311 1102 2 1.1779554609373655 1102 3 0.9903118141360387 1102 4 0.8929898142911696 1102 5 0.9392150952622309 1102 6 0.6072287746838576 1102 7 0.6444771839541552 1102 8 0.8265300827543918 1102 9 1.3550267015641282 1102 10-11 1.2808879289375454 1102 12-13 1.3752052440200444 1102 14-15 1.685499195291726 1102 16-17 1.3019801316758333 1102 18-19 1.194571864873872 1102 20-21 1.478497206268159 1102 22-23 1.4916117052328417 1102 24-25 1.2406507012270396 1102 26-27 1.5433715085732445 1102 28-29 1.2303324836229663 1102 30-31 1.2604624353248681 1102 32-33 1.3651316417136954 1102 34-35 1.3305863825564188 1102 36-37 1.1406438352670705 1102 38-39 1.432160879814468 1102 40-41 1.4084655777627404 1102 42-43 1.631488393391205 1102 44-45 1.1993025995826763 1102 46-47 1.0771406578428753 1102 48-49 1.0542393919258082 1102 50-51 1.0816408487817135 1102 52-53 0.9733879519695172 1102 54-55 0.9611285203987272 1102 56-57 0.9725419033517149 1102 58-59 0.9373402449524981 1102 60-61 0.9105430819050184 1102 62-63 0.7657133335981641 1102 64-65 0.6421101105487708 1102 66-67 0.5230017192019503 1102 68-69 0.5027901406864892 1102 70-71 0.7548138080349176 1102 72-73 0.4795500944611817 1102 74-75 0.6569220977114618 1102 76-77 0.4978111320288221 1102 78-79 0.5784838246036585 1102 80-81 0.8335633666633449 1102 82-83 0.7777634809038467 1102 84-85 0.7392827791317238 1102 86-87 0.8234467004944399 1102 88-89 0.7464209201345149 1102 90-91 0.8027766659431421 1102 92-93 0.8757381895883043 1102 94-95 0.8129062687660742 1102 96-97 0.5996080707958242 1102 98-99 0.8210646348794661 1102 100-101 0.9207992508682388 1102 102-103 0.7188887442650653 1102 104-105 0.8226943552983137 1102 106-107 0.7125928742181564 1102 108-109 0.8370985164213245 1102 110-111 0.7040684979357614 1102 112-113 0.5780423352951694 1102 114-115 0.6178257705346084 1102 116-117 0.7451897382990502 1102 118-119 0.787080223746635 1102 120-121 0.7197963066276891 1102 122-123 0.5829515713121225 1102 124-125 0.4581013977015651 1103 1 0.9434294922570103 1103 2 1.195838451419931 1103 3 0.9392580929447796 1103 4 0.7556195981287388 1103 5 0.8854670632521398 1103 6 0.8849169695649799 1103 7 0.7741996062499013 1103 8 1.049385867976536 1103 9 1.4811791335919224 1103 10-11 1.6074313487970358 1103 12-13 1.5545864646622576 1103 14-15 1.7458414364556347 1103 16-17 1.40107054872081 1103 18-19 1.374159082581123 1103 20-21 1.596323063486068 1103 22-23 1.421690643194431 1103 24-25 1.3125573247619826 1103 26-27 1.5336255585409404 1103 28-29 1.5330569277322823 1103 30-31 1.5655787200283413 1103 32-33 1.7593830697041142 1103 34-35 1.5392033208207678 1103 36-37 1.3505711650753938 1103 38-39 1.3729064566921139 1103 40-41 1.4982823737256155 1103 42-43 1.7359926805753965 1103 44-45 1.488583382368116 1103 46-47 1.3832317109908558 1103 48-49 1.3993868346449823 1103 50-51 1.3860376080569203 1103 52-53 1.2338795004885448 1103 54-55 1.3204166593692115 1103 56-57 1.2461641227609626 1103 58-59 1.254262336606601 1103 60-61 1.2502564670569214 1103 62-63 1.073938197890314 1103 64-65 0.9318250652021192 1103 66-67 0.9823508924443161 1103 68-69 0.9675393087654207 1103 70-71 1.088571674450101 1103 72-73 1.0097418431826348 1103 74-75 1.0284249954319442 1103 76-77 1.0477254292885263 1103 78-79 0.9889087659833464 1103 80-81 1.0532139285391757 1103 82-83 1.0526383160066821 1103 84-85 1.0853042237531731 1103 86-87 1.0426929451494615 1103 88-89 0.9942545247755099 1103 90-91 1.068087067268074 1103 92-93 1.074959898841346 1103 94-95 1.0922793461978806 1103 96-97 1.0554887027044053 1103 98-99 0.9653503530645615 1103 100-101 0.9412084847229316 1103 102-103 0.9262828361839439 1103 104-105 0.997793680287586 1103 106-107 1.0022987018793934 1103 108-109 1.0848147496496914 1103 110-111 0.9888999147144837 1103 112-113 1.0607593379204445 1103 114-115 1.1058319646061925 1103 116-117 1.0348324731393994 1103 118-119 1.1006390614557517 1103 120-121 1.0736518949797613 1103 122-123 1.071570069979355 1103 124-125 1.021960089799613 1104 1 1.0733250605457663 1104 2 1.4118024139848515 1104 3 0.9396386952986084 1104 4 1.1448702860002982 1104 5 0.9010505230104933 1104 6 0.8542866097746433 1104 7 0.6926179728519273 1104 8 1.0053010324750637 1104 9 1.3598182495591686 1104 10-11 1.4646162883585845 1104 12-13 1.413807062715648 1104 14-15 1.37309078973027 1104 16-17 1.1415829483273967 1104 18-19 1.2429825899363394 1104 20-21 1.5338309443107434 1104 22-23 1.4516522942720016 1104 24-25 1.3379939722226624 1104 26-27 1.5506104777948337 1104 28-29 1.545135660323119 1104 30-31 1.6338717898136252 1104 32-33 1.5971869874860403 1104 34-35 1.5671613344414865 1104 36-37 1.5235651384843294 1104 38-39 1.6301776373624293 1104 40-41 1.651036232509199 1104 42-43 1.9501442193769272 1104 44-45 1.6222787524914573 1104 46-47 1.4350076506838008 1104 48-49 1.3811910888966388 1104 50-51 1.314613030369582 1104 52-53 1.1750778470749417 1104 54-55 1.3022054837957207 1104 56-57 1.3741590020499537 1104 58-59 1.2928897424979482 1104 60-61 1.285574839100864 1104 62-63 1.2163807027117173 1104 64-65 1.0601464595008494 1104 66-67 1.0661872712601266 1104 68-69 1.074216308437581 1104 70-71 1.1194279235624727 1104 72-73 1.0250319703509412 1104 74-75 0.9390365341538356 1104 76-77 0.8656179043121206 1104 78-79 1.0311890585461043 1104 80-81 1.0694736460107812 1104 82-83 1.100489687967002 1104 84-85 1.1411472496712953 1104 86-87 1.0211082788783301 1104 88-89 1.00455326941821 1104 90-91 1.0270970730949074 1104 92-93 0.9656256510005257 1104 94-95 0.9799332960340301 1104 96-97 0.9716539830095741 1104 98-99 0.9869333602147101 1104 100-101 1.0370626234334104 1104 102-103 0.9936361798436479 1104 104-105 1.1554433562280728 1104 106-107 1.173560884804779 1104 108-109 1.0902202487730115 1104 110-111 1.103993718096703 1104 112-113 1.0671822100314188 1104 114-115 1.0804776925023845 1104 116-117 1.0512849804694007 1104 118-119 1.131521109899431 1104 120-121 1.1497733612179744 1104 122-123 1.1461667210768312 1104 124-125 0.8230446737783446 1105 1 1.0352157911964106 1105 2 1.3406752225821243 1105 3 0.9656832756400355 1105 4 1.1664496797260497 1105 5 0.9969964758603673 1105 6 0.8645983043231844 1105 7 0.7339452163655338 1105 8 0.8594518934539828 1105 9 1.2699139011111313 1105 10-11 1.1192145939888576 1105 12-13 1.2781106034451497 1105 14-15 1.5928525932262971 1105 16-17 1.1465199107743587 1105 18-19 1.3977555563988986 1105 20-21 1.2351832725683778 1105 22-23 1.310199850553019 1105 24-25 0.9906092228274304 1105 26-27 1.312859406839749 1105 28-29 1.3881880547411285 1105 30-31 1.3304651691782254 1105 32-33 1.4422016819860417 1105 34-35 1.2167683954648538 1105 36-37 0.8201170398926081 1105 38-39 1.1339082586313296 1105 40-41 1.2787262328412794 1105 42-43 1.4764356238926872 1105 44-45 1.2680131007095916 1105 46-47 0.89887760467807 1105 48-49 1.001133907430745 1105 50-51 1.1313729026979438 1105 52-53 0.947724204550429 1105 54-55 1.0226229838531928 1105 56-57 1.045469028539646 1105 58-59 1.0176595542091 1105 60-61 1.0867552525648847 1105 62-63 0.9456985611591691 1105 64-65 0.7262945718866334 1105 66-67 0.6848748946722125 1105 68-69 0.5332106068949436 1105 70-71 0.8233620071391172 1105 72-73 0.7211655478901307 1105 74-75 0.8584613731593365 1105 76-77 0.8658878782311383 1105 78-79 0.8347768858036702 1105 80-81 0.9370915915651352 1105 82-83 0.8684411175675102 1105 84-85 0.8697364499481957 1105 86-87 0.9249870265825137 1105 88-89 0.9193990889255552 1105 90-91 0.732312854259888 1105 92-93 0.8459893670166991 1105 94-95 0.7238000505739315 1105 96-97 0.7546083630051328 1105 98-99 0.8691126548276173 1105 100-101 0.8685173150724239 1105 102-103 0.6679400271076972 1105 104-105 0.7824023035884302 1105 106-107 0.7874699219542123 1105 108-109 0.8808170779285369 1105 110-111 0.8739444873707001 1105 112-113 0.8397401195168754 1105 114-115 0.852578354617016 1105 116-117 0.8626346776388054 1105 118-119 0.8294622833647765 1105 120-121 0.7305388290791015 1105 122-123 0.678878906846986 1105 124-125 0.6410159961939392 1106 1 0.08921658285774114 1106 2 -2.0361603308115654 1106 3 -0.3068407636261199 1106 4 0.1563745763079929 1106 5 0.374195923232854 1106 6 0.3927902610072067 1106 7 0.3544252923257112 1106 8 0.48961492716898647 1106 9 0.9557975477771379 1106 10-11 1.158905523632022 1106 12-13 1.0599723618115604 1106 14-15 1.1359592897046369 1106 16-17 0.7129341701364922 1106 18-19 0.7763657454917237 1106 20-21 1.0348061279354113 1106 22-23 0.8073966385821691 1106 24-25 0.8948444574070038 1106 26-27 0.8689094436437159 1106 28-29 0.9562026031302722 1106 30-31 0.9915784605823745 1106 32-33 1.1529796688877028 1106 34-35 0.9180872117315602 1106 36-37 0.7078630406670641 1106 38-39 0.9783299942489947 1106 40-41 1.0675514940234976 1106 42-43 1.3209611537422852 1106 44-45 1.108366440227595 1106 46-47 1.0248212339692913 1106 48-49 0.7239043505184917 1106 50-51 0.8395647089110376 1106 52-53 0.7653005447473795 1106 54-55 0.7651822732373468 1106 56-57 0.774089028951142 1106 58-59 0.7204367101427351 1106 60-61 0.7947635763709471 1106 62-63 0.7751627956014744 1106 64-65 0.6787014618582994 1106 66-67 0.6862511662667146 1106 68-69 0.6454914759290382 1106 70-71 0.6603858702928704 1106 72-73 0.4955691686505723 1106 74-75 0.5458855811064751 1106 76-77 0.44637516144168643 1106 78-79 0.6414929100055389 1106 80-81 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1.607996607013785 2101 50-51 1.7249517976315296 2101 52-53 1.7105746541282123 2101 54-55 1.6370433091000542 2101 56-57 1.734625249775437 2101 58-59 1.7885156492705434 2101 60-61 1.7892965723880394 2101 62-63 1.6750366629144366 2101 64-65 1.5316817311717301 2101 66-67 1.3167406500646344 2101 68-69 1.2526828359398046 2101 70-71 1.266331977812758 2101 72-73 1.1506773837796267 2101 74-75 1.1879472359761323 2101 76-77 1.3422370376770516 2101 78-79 1.3253419109700495 2101 80-81 1.4350165427689028 2101 82-83 1.447093573073726 2101 84-85 1.3584770340558343 2101 86-87 1.4896273805607798 2101 88-89 1.3909269444709373 2101 90-91 1.4453623157107387 2101 92-93 1.3323023994241723 2101 94-95 1.3424633762920521 2101 96-97 1.3220309150979261 2101 98-99 1.2485686339722015 2101 100-101 1.2916266944362356 2101 102-103 1.261900022511174 2101 104-105 1.2792875738134697 2101 106-107 1.2578670757487842 2101 108-109 1.3149821351325564 2101 110-111 1.1824350837904554 2101 112-113 1.2684843695501513 2101 114-115 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-2.0597284477591593 2216 86-87 -2.21589896260328 2216 88-89 -2.230454477823347 2216 90-91 -2.134709517511183 2216 92-93 -2.1282525984624385 2216 94-95 -2.306758247588217 2216 96-97 -2.3337320923289937 2216 98-99 -2.405319845016603 2216 100-101 -2.425938758023964 2216 102-103 -2.1912887782479764 2216 104-105 -2.3117596304363026 2216 106-107 -2.283755790618862 2216 108-109 -2.2898686702589295 2216 110-111 -2.143245556396913 2216 112-113 -2.3343931070961546 2216 114-115 -2.3112767672995282 2216 116-117 -2.11581470964094 2216 118-119 -2.131505246806089 2216 120-121 -2.307367504542974 2216 122-123 -2.179791854860433 2216 124-125 -2.017325916012812 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 202347.0 3 27047.0 4 16833.0 5 13855.0 6 13655.0 7 15283.0 8 16657.0 9 17996.0 10 19294.0 11 20234.0 12 21072.0 13 21648.0 14 21598.0 15 19014.0 16 16226.0 17 14423.0 18 12996.0 19 12356.0 20 12245.0 21 12927.0 22 16371.0 23 23216.0 24 33013.0 25 43354.0 26 50421.0 27 54683.0 28 64148.0 29 73553.0 30 86151.0 31 100503.0 32 122848.0 33 155909.0 34 204986.0 35 283777.0 36 429658.0 37 534308.0 38 152883.0 39 230.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29976051032408 19.504467436145966 13.920756664056865 23.27501538947309 2 13.369462619237659 20.409657297115928 42.62232186584376 23.598558217802655 3 19.129451107879145 25.445394781236974 28.34653492541403 27.078619185469847 4 11.8627931301631 16.92396627805853 38.027473244694285 33.18576734708408 5 13.333193148630942 38.70342490446808 34.28987912800141 13.673502818899566 6 31.310615785214598 37.721266677600944 17.75440161249002 13.213715924694439 7 28.742498535369844 32.78728352020851 20.619891795258766 17.850326149162882 8 24.90637922191258 37.20608631608759 19.86972703453687 18.017807427462955 9 25.442341693183963 17.65203277812163 20.280522669365993 36.625102859328415 10-11 23.894975504656223 28.020577476946578 27.96037813179221 20.124068886604984 12-13 24.66451786057483 25.062207851693437 28.227641367819217 22.045632919912524 14-15 21.862507528085093 26.5812873990864 25.78171838830896 25.774486684519548 16-17 21.999368461866016 29.93834460817969 26.12810403955833 21.934182890395963 18-19 21.94338153379559 28.7682614673259 27.882010200103448 21.40634679877507 20-21 22.690179518261225 27.92179069152278 27.850224537538022 21.537805252677973 22-23 22.440381303544267 27.740803432727894 27.5630149045259 22.255800359201938 24-25 22.257453089980082 27.825343495314968 27.720808254557877 22.196395160147077 26-27 22.27283642093879 27.98565073062211 27.591094966384894 22.15041788205421 28-29 22.028092398179915 27.941757440299547 27.90379245174069 22.126357709779846 30-31 22.226345636306753 27.838327254307348 27.74467007344944 22.190657035936457 32-33 22.08192687012325 27.813251042885767 27.71799826905521 22.38682381793577 34-35 22.426866759701962 27.758591529409138 27.62655545320507 22.187986257683832 36-37 22.35571449346836 27.677137979880168 27.552889434708906 22.414258091942568 38-39 22.215611728382203 27.840148436352884 27.82162098905746 22.122618846207452 40-41 22.376582106946362 27.743447976800283 27.77432785863349 22.10564205761986 42-43 22.18164775756117 27.531177353482327 27.87968571363193 22.40748917532457 44-45 22.455626356659767 27.75623227420669 27.57931511996517 22.20882624916837 46-47 22.330848440859917 28.02470095198623 27.178344239128023 22.46610636802583 48-49 22.363384985156635 27.8347579601723 27.338799548011274 22.46305750665979 50-51 22.311031524009834 27.88962753033421 27.39672518192399 22.402615763731966 52-53 22.49637664101158 27.948994215157917 27.164252053688376 22.390377090142128 54-55 22.379474504674786 27.79480329739291 27.334267601431478 22.49145459650083 56-57 22.32623730136072 28.147740245877017 27.261591376524542 22.264431076237727 58-59 22.49781661458537 27.711092613631266 27.250545994480213 22.54054477730315 60-61 22.53601179345072 27.70770134884122 27.47632243310523 22.279964424602827 62-63 22.37942940878917 27.788710200582496 27.44029283243124 22.391567558197092 64-65 22.31353599811485 27.6786769289003 27.347015333423847 22.660771739561007 66-67 22.277832374237345 27.919307297874713 27.208947740976043 22.5939125869119 68-69 22.240585910747733 27.80403311286751 27.286851911221007 22.66852906516375 70-71 22.1675066129584 27.774306800983595 27.418856584072465 22.639330001985538 72-73 22.384034501700953 27.7303156755359 27.17291567736361 22.71273414539954 74-75 22.257616861259145 27.852589216660206 27.186732623334514 22.703061298746135 76-77 22.358204897779967 27.70406132880546 27.381922261754454 22.555811511660128 78-79 22.382701363836652 27.75235853328524 27.252068980168705 22.61287112270941 80-81 22.109040993940663 27.93242850426571 27.393722877432957 22.564807624360668 82-83 22.41332022714829 27.98089806261646 27.1525910054234 22.453190704811853 84-85 22.38256092967748 27.685700622521658 27.305907396200546 22.62583105160032 86-87 22.23444917907433 27.59949636752708 27.59365474558128 22.572399707817308 88-89 22.411361399105413 27.600998548298783 27.37811314058814 22.60952691200767 90-91 22.278988380337246 27.84281865023928 27.491533607228448 22.386659362195026 92-93 22.344718606781527 27.670814230482925 27.476192435178127 22.50827472755742 94-95 22.189716131916366 27.668722356558906 27.407714086386388 22.733847425138347 96-97 22.346357837677864 27.70145507702383 27.30860696802122 22.643580117277086 98-99 22.203094755622253 27.616639970419392 27.261357812728292 22.918907461230056 100-101 22.105364007049072 27.62047819051957 27.474872324035175 22.799285478396182 102-103 22.203049783691995 27.50667686286675 27.41504991645031 22.87522343699094 104-105 22.1599425179864 27.369241537017587 27.647900662414195 22.822915282581814 106-107 22.007858081244862 27.622843246835533 27.55753697514824 22.811761696771367 108-109 22.0798520239293 27.649371472452305 27.28715107972861 22.983625423889787 110-111 21.928305881815312 27.66422461979302 27.51257342431277 22.8948960740789 112-113 22.085107650461698 27.54823869161593 27.4960626829672 22.870590974955178 114-115 21.90736810752448 27.592596353346938 27.411888346212038 23.088147192916544 116-117 21.809415173403558 27.512016057514604 27.57739356480886 23.101175204272977 118-119 21.820374229098817 27.54836925823933 27.4366366016757 23.194619910986148 120-121 21.73243950563222 27.58956706186602 27.49658926300162 23.181404169500137 122-123 21.807495511548115 27.578727310638783 27.403183483580257 23.21059369423285 124-125 21.773728543776933 27.482328227938485 27.433163578489207 23.31077964979538 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5046.0 1 3741.0 2 1877.5 3 1068.5 4 729.0 5 611.0 6 580.5 7 671.5 8 810.0 9 841.0 10 837.0 11 900.5 12 995.5 13 1045.5 14 1132.5 15 1264.0 16 1391.5 17 1558.0 18 1744.5 19 2012.5 20 2486.0 21 3137.0 22 4018.0 23 5373.5 24 7051.5 25 9441.5 26 13593.0 27 18232.5 28 22404.0 29 27635.5 30 34081.0 31 40235.5 32 46629.5 33 54514.5 34 62992.5 35 70240.5 36 78611.5 37 87159.5 38 92522.0 39 95972.0 40 98384.5 41 102090.0 42 105915.0 43 109660.0 44 115139.0 45 118832.0 46 118484.0 47 116804.0 48 117368.5 49 116646.0 50 113214.5 51 107951.5 52 101673.0 53 97068.5 54 92223.5 55 84464.0 56 74911.0 57 66550.0 58 59620.5 59 51997.0 60 42997.5 61 34408.0 62 26806.5 63 20388.0 64 15412.0 65 11489.0 66 8537.5 67 6525.0 68 4933.5 69 3621.5 70 2631.5 71 1977.0 72 1570.5 73 1238.0 74 937.0 75 713.0 76 515.0 77 343.5 78 251.0 79 189.0 80 129.0 81 99.5 82 84.0 83 61.0 84 45.5 85 36.0 86 26.5 87 23.0 88 16.0 89 12.0 90 9.0 91 6.5 92 5.5 93 3.0 94 1.5 95 2.5 96 1.5 97 1.5 98 4.0 99 3.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2037043423341914 2 0.24353234486857775 3 0.29431473859238777 4 0.2807231791536583 5 0.311963479953126 6 0.22835172251039482 7 0.21844543665082336 8 0.20001906875503345 9 0.19849762553428013 10-11 0.14191684264693252 12-13 0.16619231448028515 14-15 0.18392558046439858 16-17 0.1558803104285128 18-19 0.18240413724364526 20-21 0.17598026586713134 22-23 0.19935977669270702 24-25 0.18945349083313553 26-27 0.24290686265560135 28-29 0.16896472212699115 30-31 0.14856047804422193 32-33 0.18842229042795833 34-35 0.14736022839229435 36-37 0.1475292776390447 38-39 0.18059531030341636 40-41 0.2014390824277365 42-43 0.2657623208162509 44-45 0.1664627932750857 46-47 0.15118074136885262 48-49 0.15373338499478315 50-51 0.15887248209599428 52-53 0.18078126447484177 54-55 0.16773066262571346 56-57 0.167054465638712 58-59 0.14073349791967998 60-61 0.1539531490155586 62-63 0.14267756425730918 64-65 0.13878943158205076 66-67 0.12580644943162264 68-69 0.12918743436662997 70-71 0.1304553037172577 72-73 0.10809008837218423 74-75 0.10998343993578834 76-77 0.1308272120601085 78-79 0.13620297810677015 80-81 0.14681927080269316 82-83 0.14893238638707273 84-85 0.13542535157171845 86-87 0.16130679124919955 88-89 0.1565734123401893 90-91 0.14457091582091328 92-93 0.14529782758193985 94-95 0.1669530360906618 96-97 0.1535305258986827 98-99 0.14982834739484968 100-101 0.13028625447050732 102-103 0.16850828916076516 104-105 0.1268038399874498 106-107 0.13332914091201392 108-109 0.14428353210143768 110-111 0.12071806710443658 112-113 0.11140345360849141 114-115 0.10913819370203649 116-117 0.11285727713054457 118-119 0.09794713356716224 120-121 0.09167540651272366 122-123 0.11732017724475424 124-125 0.11358418889157113 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2957718.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.05559389459811 #Duplication Level Percentage of deduplicated Percentage of total 1 77.61418337885083 45.059375107034306 2 11.95660416622632 13.882955116657902 3 4.399322339338224 7.662158134321598 4 2.016582301756635 4.682955326632684 5 1.1010910824743263 3.1962248362546464 6 0.6796282418928417 2.3673732726378303 7 0.47108114718160415 1.9144227040523627 8 0.2920004922084369 1.3561809594140617 9 0.23006426108123293 1.202086557989357 >10 1.1298258082236414 11.702200844978728 >50 0.07403039755037338 2.9406295817108434 >100 0.03468477554485146 3.5113996937828342 >500 7.212861364940781E-4 0.27138291930901465 >1k 1.8032153407835595E-4 0.25065494522369736 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3213 0.1086310459617854 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 3.3809849350073266E-5 12-13 0.0 0.0 0.0 0.0 3.3809849350073266E-5 14-15 0.0 0.0 0.0 0.0 3.3809849350073266E-5 16-17 0.0 0.0 0.0 3.3809849350073266E-5 5.07147740251099E-5 18-19 0.0 0.0 0.0 3.3809849350073266E-5 6.761969870014653E-5 20-21 0.0 0.0 0.0 3.3809849350073266E-5 6.761969870014653E-5 22-23 0.0 0.0 0.0 1.6904924675036634E-4 6.761969870014653E-5 24-25 0.0 0.0 0.0 3.888132675258426E-4 6.761969870014653E-5 26-27 0.0 0.0 0.0 6.761969870014654E-4 6.761969870014653E-5 28-29 0.0 0.0 0.0 0.001149534877902491 6.761969870014653E-5 30-31 0.0 0.0 0.0 0.003076696290856667 6.761969870014653E-5 32-33 0.0 0.0 0.0 0.006998638815465166 6.761969870014653E-5 34-35 0.0 0.0 0.0 0.011258679833574398 6.761969870014653E-5 36-37 0.0 3.3809849350073266E-5 0.0 0.018544702368515187 6.761969870014653E-5 38-39 0.0 3.3809849350073266E-5 0.0 0.03303222281502158 6.761969870014653E-5 40-41 0.0 3.3809849350073266E-5 0.0 0.05037667553160917 6.761969870014653E-5 42-43 0.0 3.3809849350073266E-5 0.0 0.06643635397289396 6.761969870014653E-5 44-45 0.0 3.3809849350073266E-5 0.0 0.0864348798634623 6.761969870014653E-5 46-47 0.0 3.3809849350073266E-5 0.0 0.1096453414422876 6.761969870014653E-5 48-49 0.0 3.3809849350073266E-5 0.0 0.13579725991456926 6.761969870014653E-5 50-51 0.0 3.3809849350073266E-5 0.0 0.1664458883504107 6.761969870014653E-5 52-53 0.0 3.3809849350073266E-5 0.0 0.19582664743562436 6.761969870014653E-5 54-55 0.0 3.3809849350073266E-5 0.0 0.22634003647406548 6.761969870014653E-5 56-57 0.0 3.3809849350073266E-5 0.0 0.26518755337729966 6.761969870014653E-5 58-59 0.0 3.3809849350073266E-5 0.0 0.30758510446229154 6.761969870014653E-5 60-61 0.0 3.3809849350073266E-5 0.0 0.3574039174796245 6.761969870014653E-5 62-63 0.0 3.3809849350073266E-5 0.0 0.4058027168242544 6.761969870014653E-5 64-65 0.0 3.3809849350073266E-5 0.0 0.45641606130131407 6.761969870014653E-5 66-67 0.0 3.3809849350073266E-5 0.0 0.5150930548483662 6.761969870014653E-5 68-69 0.0 3.3809849350073266E-5 0.0 0.5830508520420135 6.761969870014653E-5 70-71 0.0 3.3809849350073266E-5 0.0 0.6614220828354833 6.761969870014653E-5 72-73 0.0 3.3809849350073266E-5 0.0 0.7430390591665601 6.761969870014653E-5 74-75 0.0 3.3809849350073266E-5 0.0 0.8291527454611968 6.761969870014653E-5 76-77 0.0 3.3809849350073266E-5 0.0 0.9216057785089722 6.761969870014653E-5 78-79 0.0 3.3809849350073266E-5 0.0 1.03370233402914 6.761969870014653E-5 80-81 0.0 3.3809849350073266E-5 0.0 1.1612330857776163 6.761969870014653E-5 82-83 0.0 3.3809849350073266E-5 0.0 1.2952722335259819 6.761969870014653E-5 84-85 0.0 3.3809849350073266E-5 0.0 1.4329121302301302 6.761969870014653E-5 86-87 0.0 3.3809849350073266E-5 0.0 1.5775337608250686 6.761969870014653E-5 88-89 0.0 3.3809849350073266E-5 0.0 1.7449601348066313 6.761969870014653E-5 90-91 0.0 3.3809849350073266E-5 0.0 1.9415136940032822 6.761969870014653E-5 92-93 0.0 3.3809849350073266E-5 0.0 2.1562907619996228 6.761969870014653E-5 94-95 0.0 3.3809849350073266E-5 0.0 2.371000210297263 6.761969870014653E-5 96-97 0.0 3.3809849350073266E-5 0.0 2.592150434896092 6.761969870014653E-5 98-99 0.0 3.3809849350073266E-5 0.0 2.839875201084079 6.761969870014653E-5 100-101 0.0 3.3809849350073266E-5 0.0 3.127715353525928 6.761969870014653E-5 102-103 0.0 6.761969870014653E-5 0.0 3.4410312274530566 6.761969870014653E-5 104-105 0.0 8.452462337518317E-5 0.0 3.752656608912682 6.761969870014653E-5 106-107 0.0 1.014295480502198E-4 0.0 4.068339172294316 6.761969870014653E-5 108-109 0.0 1.1833447272525642E-4 0.0 4.40939602761318 8.452462337518317E-5 110-111 0.0 1.3523939740029306E-4 0.0 4.803416011938934 1.014295480502198E-4 112-113 0.0 1.3523939740029306E-4 0.0 5.22855796259143 1.014295480502198E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7475 0.0 39.53043 1 ATCAACG 8000 0.0 35.221684 3 GTACATG 12275 0.0 34.89542 1 CAACGCA 8250 0.0 34.009945 5 TCAACGC 8340 0.0 33.571503 4 TACATGG 12440 0.0 33.23365 2 AACGCAG 8605 0.0 31.886988 6 TATCAAC 9280 0.0 31.06965 2 ACATGGG 12525 0.0 30.43976 3 GGTATCA 5195 0.0 27.407753 1 ACGCAGA 9850 0.0 27.066471 7 CGCAGAG 9580 0.0 26.148548 8 CATGGGA 8180 0.0 24.46949 4 GTATAGG 955 0.0 23.081255 1 CATGGGG 5225 0.0 21.776396 4 GCAGAGT 11060 0.0 21.57458 9 GTGTAGC 1950 0.0 21.38575 1 GGACCGA 395 1.4551915E-11 21.095722 6 GTACCTG 2855 0.0 20.866756 1 AGTACAT 9370 0.0 20.090302 2 >>END_MODULE