FastQCFastQC Report
Thu 26 May 2016
SRR1364618_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364618_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2957718
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65380.221048795050779No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55600.18798276238640735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35940.12151259856416333No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA106300.040.5808221
GGTATCA72750.039.803281
ATCAACG124450.033.8517153
TCAACGC126450.033.3185544
CAACGCA126150.032.8650745
TATCAAC133550.031.9693262
AACGCAG131100.031.613476
GTACATG127500.028.7395861
TACATGG129500.027.6812062
ACGCAGA152300.027.0178687
CGCAGAG153500.026.767868
ACATGGG129500.026.144773
GAGTACT86850.023.96755812-13
GCAGAGT173050.022.7462679
GTACTTT91250.022.64868714-15
AGTACTT90200.022.28505312-13
CATGGGA89850.021.2597754
AGTACAT98250.020.303482
CGTAGAC2701.1059492E-619.8344923
CAGAGTA167100.019.66541110-11
Produced by FastQC (version 0.11.5)