##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364618_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2957718 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.735247579383834 31.0 30.0 33.0 26.0 34.0 2 29.79503252169409 31.0 30.0 34.0 26.0 34.0 3 29.824758141242675 31.0 30.0 34.0 26.0 34.0 4 33.49078918274156 35.0 33.0 37.0 28.0 37.0 5 32.910835989097 35.0 33.0 37.0 26.0 37.0 6 32.92699202560893 35.0 33.0 37.0 26.0 37.0 7 32.720796235476136 35.0 33.0 37.0 25.0 37.0 8 32.64731018981526 35.0 33.0 37.0 25.0 37.0 9 33.95383941268234 38.0 33.0 39.0 25.0 39.0 10-11 33.73123959079263 37.0 33.0 39.0 23.5 39.0 12-13 33.602549499309944 37.0 33.0 39.0 22.5 39.0 14-15 34.58884974835329 38.0 33.0 41.0 22.0 41.0 16-17 34.488494677315416 38.0 33.0 41.0 20.5 41.0 18-19 34.52268962084959 38.5 33.0 41.0 22.0 41.0 20-21 34.54440365849618 39.0 33.5 41.0 22.0 41.0 22-23 34.537314240235204 39.0 34.0 41.0 22.0 41.0 24-25 34.54213298901383 39.0 34.0 41.0 22.0 41.0 26-27 34.45095847541923 39.0 33.5 41.0 21.5 41.0 28-29 34.21681918289708 39.0 33.0 41.0 19.0 41.0 30-31 34.17356184734312 38.0 33.0 41.0 19.0 41.0 32-33 34.02842596893957 38.0 33.0 41.0 18.5 41.0 34-35 33.92077777529839 38.0 33.0 41.0 18.0 41.0 36-37 33.781576877849744 38.0 33.0 40.0 16.0 41.0 38-39 33.66824237469562 38.0 33.0 40.0 16.0 41.0 40-41 33.518817885951265 38.0 33.0 40.0 15.0 41.0 42-43 33.371517839090814 38.0 32.0 40.0 14.0 41.0 44-45 33.19777494000442 38.0 32.0 40.0 9.0 41.0 46-47 32.98247584793412 38.0 32.0 40.0 8.5 41.0 48-49 32.76680248083151 38.0 31.5 40.0 7.0 41.0 50-51 32.59088442508718 38.0 31.0 40.0 2.0 41.0 52-53 32.43641415442581 38.0 31.0 40.0 2.0 41.0 54-55 32.22868576382197 38.0 31.0 40.0 2.0 41.0 56-57 32.01366002438366 37.0 31.0 40.0 2.0 41.0 58-59 31.83667290120289 37.0 30.0 40.0 2.0 41.0 60-61 31.627238296551596 37.0 30.0 40.0 2.0 41.0 62-63 31.3692686388628 36.0 30.0 40.0 2.0 41.0 64-65 31.104758972964966 36.0 29.0 39.0 2.0 41.0 66-67 30.837607574488167 35.5 29.0 39.0 2.0 41.0 68-69 30.52135058176608 35.0 29.0 39.0 2.0 40.5 70-71 30.26615924844762 35.0 29.0 38.5 2.0 40.0 72-73 30.101532329992246 35.0 29.0 37.5 2.0 40.0 74-75 29.807793373134288 35.0 29.0 37.0 2.0 39.5 76-77 29.505969636050494 35.0 29.0 37.0 2.0 39.0 78-79 29.194818606777254 34.0 29.0 36.0 2.0 39.0 80-81 28.90713583918413 34.0 29.0 36.0 2.0 37.5 82-83 28.64282531329897 34.0 28.0 35.0 2.0 37.0 84-85 28.61050935214243 34.0 29.0 35.0 2.0 37.0 86-87 28.45335998901856 34.0 29.0 35.0 2.0 36.0 88-89 28.310461646445 34.0 29.0 35.0 2.0 36.0 90-91 28.15089944342226 34.0 29.0 35.0 2.0 36.0 92-93 28.014658767333465 34.0 28.0 35.0 2.0 35.5 94-95 27.928090507614318 34.0 28.0 35.0 2.0 35.0 96-97 27.84581390112242 34.0 28.0 35.0 2.0 35.0 98-99 27.753593986985912 34.0 27.5 35.0 2.0 35.0 100-101 27.654382026954565 34.0 27.0 35.0 2.0 35.0 102-103 26.464623064132553 32.5 25.0 34.0 2.0 35.0 104-105 27.402693055930282 34.0 27.0 35.0 2.0 35.0 106-107 27.578579499465466 34.0 27.0 35.0 2.0 35.0 108-109 27.588128077118917 34.0 27.0 35.0 2.0 35.0 110-111 27.535969791575802 34.0 27.0 35.0 2.0 35.0 112-113 27.490671355416573 34.0 27.0 35.0 2.0 35.0 114-115 27.41445871445486 34.0 27.0 35.0 2.0 35.0 116-117 27.29790094931295 34.0 27.0 35.0 2.0 35.0 118-119 27.195008110982858 34.0 27.0 35.0 2.0 35.0 120-121 27.061783442505337 34.0 27.0 35.0 2.0 35.0 122-123 26.881110876696155 34.0 27.0 35.0 2.0 35.0 124-125 26.066597626954294 33.0 23.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7773056128950415 1101 2 0.7899630086676339 1101 3 0.6569551815989989 1101 4 0.7417497596892204 1101 5 0.9750992437306607 1101 6 0.9767955074575241 1101 7 1.044616103101042 1101 8 1.1611321680537614 1101 9 1.114135438273756 1101 10-11 1.2451889788458246 1101 12-13 1.1937667859179228 1101 14-15 1.3053828687788638 1101 16-17 1.3828111156871614 1101 18-19 1.40473686354499 1101 20-21 1.4419641851004101 1101 22-23 1.2595257844255485 1101 24-25 1.18011027552528 1101 26-27 1.293944687752564 1101 28-29 1.4680012183278635 1101 30-31 1.3269083237170562 1101 32-33 1.4577949222346547 1101 34-35 1.367007010126592 1101 36-37 1.4044649221020435 1101 38-39 1.45952551719283 1101 40-41 1.492015394434823 1101 42-43 1.551971693960141 1101 44-45 1.5030606347044326 1101 46-47 1.5844866770595374 1101 48-49 1.6857993654953134 1101 50-51 1.6183744008001568 1101 52-53 1.55607129952908 1101 54-55 1.6175872561889122 1101 56-57 1.6015318784471475 1101 58-59 1.5805502869081494 1101 60-61 1.5279999142086957 1101 62-63 1.4754449520799433 1101 64-65 1.4926016627869565 1101 66-67 1.5263591701456143 1101 68-69 1.45928891328699 1101 70-71 1.5297359850865107 1101 72-73 1.508919876391591 1101 74-75 1.4835678909285974 1101 76-77 1.483401331153484 1101 78-79 1.3544737887167813 1101 80-81 1.40488307792943 1101 82-83 1.3724967568442352 1101 84-85 1.4631769960165215 1101 86-87 1.502569188265717 1101 88-89 1.3994415458111753 1101 90-91 1.4364824067522868 1101 92-93 1.3426739517215367 1101 94-95 1.3147775458818138 1101 96-97 1.3181058205257727 1101 98-99 1.3817600539180148 1101 100-101 1.3975703056431037 1101 102-103 1.436441491211518 1101 104-105 1.3651388652220646 1101 106-107 1.2643935683993952 1101 108-109 1.2884260629339472 1101 110-111 1.302260259703381 1101 112-113 1.2706077083524399 1101 114-115 1.2731231114659423 1101 116-117 1.3894232463306402 1101 118-119 1.3719214605667496 1101 120-121 1.2719702813576887 1101 122-123 1.2900635659173965 1101 124-125 1.0828482251470462 1102 1 0.3806090367046018 1102 2 -0.479244318928604 1102 3 -0.03800566518799542 1102 4 0.1994813710332508 1102 5 0.6035979925106219 1102 6 0.4836307097639221 1102 7 0.5066022926488856 1102 8 0.4937256202907676 1102 9 0.6847570585929574 1102 10-11 0.8033782093220623 1102 12-13 0.781268546161165 1102 14-15 0.8799919018013043 1102 16-17 0.9501673124987349 1102 18-19 1.0236502677379704 1102 20-21 0.9081014885941343 1102 22-23 0.90906433328378 1102 24-25 0.914170025541523 1102 26-27 0.9547000248045521 1102 28-29 1.0487712129542288 1102 30-31 0.8897785606256718 1102 32-33 0.8454579356453706 1102 34-35 0.8130208876251288 1102 36-37 0.8274490838276236 1102 38-39 0.7749869093381889 1102 40-41 0.6200176360777121 1102 42-43 0.46425172971968465 1102 44-45 0.3927040802753936 1102 46-47 0.47320613966513747 1102 48-49 0.6094226733322756 1102 50-51 0.4764696560665769 1102 52-53 0.46399097708008696 1102 54-55 0.5277704108072427 1102 56-57 0.29043985938170636 1102 58-59 0.4154761372476159 1102 60-61 0.35932571563075655 1102 62-63 0.4864233561973883 1102 64-65 0.4732947305107551 1102 66-67 0.5260924181712419 1102 68-69 0.28152805516386437 1102 70-71 0.1671365471954438 1102 72-73 0.2043600551937068 1102 74-75 0.2701055782187538 1102 76-77 0.32937646400291243 1102 78-79 0.3836929994022107 1102 80-81 0.36093469768954733 1102 82-83 0.33612679578516946 1102 84-85 0.35747503747919396 1102 86-87 0.306164666340063 1102 88-89 0.44851552829685204 1102 90-91 0.43075570280650766 1102 92-93 0.4293320357895425 1102 94-95 0.2852154392624051 1102 96-97 0.26525524099911024 1102 98-99 0.33438210783323186 1102 100-101 0.19216224294061846 1102 102-103 0.22108230620441205 1102 104-105 0.20020054513756236 1102 106-107 0.47225566104713224 1102 108-109 0.5316580999894249 1102 110-111 0.42036601963829057 1102 112-113 0.4246137187077963 1102 114-115 0.4858618911489927 1102 116-117 0.5616895816911338 1102 118-119 0.44055826534786036 1102 120-121 0.4963306638264271 1102 122-123 0.4508912976246755 1102 124-125 0.3676949210560778 1103 1 0.3035449233665801 1103 2 -0.9109740198441791 1103 3 0.0571316553841541 1103 4 0.32329376738786664 1103 5 0.6255496311668054 1103 6 0.543465507226017 1103 7 0.5368924195407985 1103 8 0.680015831799281 1103 9 0.705295691018101 1103 10-11 0.7964618543094844 1103 12-13 0.8629024108162255 1103 14-15 0.9291325656390015 1103 16-17 1.0492171272236348 1103 18-19 0.9916981468581625 1103 20-21 1.00496875801759 1103 22-23 0.8720846329652971 1103 24-25 0.7500692094121462 1103 26-27 0.9246803525824205 1103 28-29 0.9769221093727012 1103 30-31 0.9178581641468568 1103 32-33 0.93944627879889 1103 34-35 0.9144271154296248 1103 36-37 1.0574544599387465 1103 38-39 1.1000252000555832 1103 40-41 1.1028554789412723 1103 42-43 1.0330730887236967 1103 44-45 1.046743396969056 1103 46-47 1.015290415978825 1103 48-49 0.9771398027687184 1103 50-51 0.9648807928849052 1103 52-53 1.0465278737276265 1103 54-55 1.0551653193218016 1103 56-57 1.0517809005594003 1103 58-59 0.9876588736187983 1103 60-61 0.9357652697513181 1103 62-63 0.9339257505400802 1103 64-65 0.9503438425994339 1103 66-67 0.958528167274384 1103 68-69 0.9006096557050469 1103 70-71 0.9126386176294403 1103 72-73 0.9146491664589611 1103 74-75 0.8726091775518796 1103 76-77 0.7091451752181719 1103 78-79 0.7500368719377661 1103 80-81 0.8556053019851326 1103 82-83 0.8121143334887435 1103 84-85 0.8895031313276505 1103 86-87 0.8712936594559757 1103 88-89 0.8278918357171463 1103 90-91 0.8866334061163705 1103 92-93 0.8288022551250975 1103 94-95 0.819666450970999 1103 96-97 0.8411285235465655 1103 98-99 0.848821947648549 1103 100-101 0.8232827725768423 1103 102-103 0.8829660593736968 1103 104-105 0.8653358958716595 1103 106-107 0.7680763175286422 1103 108-109 0.8234160792267389 1103 110-111 0.7841524862367457 1103 112-113 0.8475771569441051 1103 114-115 0.7385457101918433 1103 116-117 0.7544476461137073 1103 118-119 0.8441988376881753 1103 120-121 0.7820995449572763 1103 122-123 0.6829200226856678 1103 124-125 0.5747503475447857 1104 1 0.36557016589688374 1104 2 -0.7270647724347299 1104 3 0.13389536735544283 1104 4 0.36375956228600614 1104 5 0.6559238226771456 1104 6 0.5266765301603726 1104 7 0.6593997561737979 1104 8 0.5864470492888074 1104 9 0.7491812660893444 1104 10-11 0.7641474867199491 1104 12-13 0.7155993451588429 1104 14-15 0.8261894177422988 1104 16-17 0.8718444846686424 1104 18-19 0.7729826980526511 1104 20-21 0.7916938157881859 1104 22-23 0.8009036142384645 1104 24-25 0.7140390744658802 1104 26-27 0.6170068184821744 1104 28-29 1.0117058296794212 1104 30-31 0.8750880161134518 1104 32-33 0.7840950661141761 1104 34-35 0.8300030759611303 1104 36-37 0.9121147150982765 1104 38-39 0.8651245283106732 1104 40-41 0.9279012540892282 1104 42-43 0.8259757175677152 1104 44-45 0.7902379835891082 1104 46-47 0.7749058516440854 1104 48-49 0.7854125284124862 1104 50-51 0.7911153282182113 1104 52-53 0.676029483738624 1104 54-55 0.8223918895470845 1104 56-57 0.7965307781162707 1104 58-59 0.8235633467417003 1104 60-61 0.6903960423309634 1104 62-63 0.7456550058867712 1104 64-65 0.6812763946858773 1104 66-67 0.6954540450489297 1104 68-69 0.5843777119187195 1104 70-71 0.7393684005765948 1104 72-73 0.6674961351121986 1104 74-75 0.7959521470598858 1104 76-77 0.7942551782576821 1104 78-79 0.7358929485015544 1104 80-81 0.6546256905910859 1104 82-83 0.7322936911036493 1104 84-85 0.7049748658789099 1104 86-87 0.7541214752165644 1104 88-89 0.7522999608971617 1104 90-91 0.7285843797549276 1104 92-93 0.7911816038471073 1104 94-95 0.7537120781253641 1104 96-97 0.785948731241966 1104 98-99 0.7672320186456538 1104 100-101 0.8135511154866215 1104 102-103 0.7565692454169053 1104 104-105 0.7089705454852187 1104 106-107 0.7043536283191685 1104 108-109 0.6855454481900267 1104 110-111 0.6438928868945517 1104 112-113 0.6869197435269925 1104 114-115 0.726197619309179 1104 116-117 0.7081807346935172 1104 118-119 0.6826133949484188 1104 120-121 0.7344407139871976 1104 122-123 0.7968721849185023 1104 124-125 0.7671329746898614 1105 1 0.6623684125192568 1105 2 0.4032028322276595 1105 3 0.576231428846274 1105 4 0.5784713687196543 1105 5 0.6694439121852369 1105 6 0.6385974033751296 1105 7 0.7511209604458671 1105 8 0.7399512500893763 1105 9 0.8260585347643286 1105 10-11 1.0310081642123947 1105 12-13 0.9277978875870545 1105 14-15 0.9883468334516863 1105 16-17 1.136731845993694 1105 18-19 1.1255290422449775 1105 20-21 1.1516300374433186 1105 22-23 1.0018234414040634 1105 24-25 1.0604282251863637 1105 26-27 1.0589328900120094 1105 28-29 1.1709305718710894 1105 30-31 1.0157624687576927 1105 32-33 0.9973599336784815 1105 34-35 1.0007466521464607 1105 36-37 1.0916543959958105 1105 38-39 1.0070870646797232 1105 40-41 0.9111097065240088 1105 42-43 0.8946811263698535 1105 44-45 0.8984446798363592 1105 46-47 0.8381301991725252 1105 48-49 1.01714642193771 1105 50-51 0.963542130322061 1105 52-53 0.8907574151668456 1105 54-55 0.9123251352626269 1105 56-57 0.8781609288546406 1105 58-59 0.7772226667969804 1105 60-61 0.7791553565109872 1105 62-63 0.8428577503927777 1105 64-65 0.7994068276914739 1105 66-67 0.7272622732025944 1105 68-69 0.6831389930287841 1105 70-71 0.8351408101680597 1105 72-73 0.7277862125888035 1105 74-75 0.7321118566694906 1105 76-77 0.8273853366594075 1105 78-79 0.7513973702624419 1105 80-81 0.6332730047098138 1105 82-83 0.6865485183841997 1105 84-85 0.5927548021708091 1105 86-87 0.6871850262149728 1105 88-89 0.7265536131286083 1105 90-91 0.6439192176620168 1105 92-93 0.547914347867767 1105 94-95 0.6500497565859469 1105 96-97 0.7053427571390252 1105 98-99 0.8204681294374581 1105 100-101 0.8170476848541135 1105 102-103 0.4537641988364278 1105 104-105 0.39767034087340747 1105 106-107 0.776796363833494 1105 108-109 0.8306486864721094 1105 110-111 0.7751806768570795 1105 112-113 0.6359218139124359 1105 114-115 0.7904148514043712 1105 116-117 0.5157728291674637 1105 118-119 0.3837632826801425 1105 120-121 0.32843266873247856 1105 122-123 0.4783618725853387 1105 124-125 0.3920379809099579 1106 1 0.3220298897814118 1106 2 0.2667536451852328 1106 3 0.41867040290477675 1106 4 0.332698603773359 1106 5 0.40395469588388977 1106 6 0.42931199595359715 1106 7 0.6201008566086443 1106 8 0.5112491048345689 1106 9 0.5391098610120153 1106 10-11 0.6722657418822564 1106 12-13 0.6296478048126843 1106 14-15 0.6135812533386584 1106 16-17 0.813551077897948 1106 18-19 0.7101643329594154 1106 20-21 0.7228176031400082 1106 22-23 0.6636615267014321 1106 24-25 0.7653301688101237 1106 26-27 0.6823373991650925 1106 28-29 0.8050027788773164 1106 30-31 0.7820137896007111 1106 32-33 0.7264093904016562 1106 34-35 0.779456735510422 1106 36-37 0.7681054347518952 1106 38-39 0.7952386613758762 1106 40-41 0.8962724285492314 1106 42-43 0.8959073108908129 1106 44-45 0.7410729708889008 1106 46-47 0.5618732525593018 1106 48-49 0.6115600966128056 1106 50-51 0.6957717440447624 1106 52-53 0.7573354575277875 1106 54-55 0.7753544436214668 1106 56-57 0.5259771964816196 1106 58-59 0.48570911965367003 1106 60-61 0.7039724805939223 1106 62-63 0.7229706527220934 1106 64-65 0.6144663113059572 1106 66-67 0.42508125851878376 1106 68-69 0.48967437534624025 1106 70-71 0.6504150987092707 1106 72-73 0.702857171914669 1106 74-75 0.5597022674336287 1106 76-77 0.679416832374212 1106 78-79 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124-125 -2.3508508570761535 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 108932.0 3 34948.0 4 16369.0 5 12715.0 6 13031.0 7 15276.0 8 17242.0 9 18768.0 10 20116.0 11 21760.0 12 23453.0 13 25480.0 14 27717.0 15 23437.0 16 16883.0 17 14186.0 18 13448.0 19 12616.0 20 12321.0 21 13200.0 22 15584.0 23 20888.0 24 28235.0 25 37754.0 26 45349.0 27 51826.0 28 59721.0 29 69903.0 30 81861.0 31 95849.0 32 116727.0 33 143990.0 34 185009.0 35 251152.0 36 395827.0 37 638329.0 38 256839.0 39 977.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.90763676491533 19.662767595296756 13.588179714296636 24.84141592549128 2 15.449324624958003 20.630329737852097 39.44368897119205 24.47665666599785 3 19.214340244742736 24.894158266609594 29.203933573112785 26.68756791553488 4 12.78376728876055 17.35319029513252 36.887862491272145 32.975179924834784 5 13.179096835114676 38.54823424845726 34.33642523331297 13.936243683115087 6 31.74100586130323 37.65062359181311 17.13031459537533 13.478055951508333 7 28.343245821016684 32.65346211283906 20.71117689758689 18.29211516855737 8 25.700692222855594 36.92546077753187 19.136509971538867 18.237337028073668 9 25.98902663077399 16.588599407784276 19.78976274180057 37.63261121964116 10-11 24.657784273376755 27.41569042381246 27.322671416573417 20.603853886237363 12-13 25.39724725816296 24.484473771858195 28.09030859720398 22.02797037277487 14-15 22.446474722185545 25.922936042869928 25.88411953388406 25.74646970106047 16-17 21.954584257921606 29.2957432488776 26.52367833986317 22.225994153337624 18-19 21.820513778459684 27.87630449118737 28.86225157972482 21.44093015062813 20-21 22.477019936934482 27.08755543931233 28.939587316631616 21.495837307121576 22-23 22.273457884798205 26.866441792565897 28.78058240149106 22.07951792114483 24-25 22.011648845495074 27.100453795797975 28.92351468260328 21.96438267610367 26-27 22.003500671801707 27.306660066984072 28.733638568653262 21.956200692560955 28-29 21.775949333688754 27.124368176457974 29.09119908961932 22.008483400233956 30-31 22.026156994371505 27.0586863011765 28.913968288046444 22.001188416405558 32-33 21.879781653563953 27.111568915741802 28.841336715136816 22.167312715557426 34-35 22.149187312651172 27.071681614001065 28.79535844864182 21.98377262470594 36-37 22.152280594630056 26.92872124311269 28.755120736368355 22.163877425888895 38-39 21.998023476206995 27.22015756742191 28.870534648671715 21.911284307699383 40-41 22.148481049998857 27.080375323201082 28.778272434740643 21.992871192059418 42-43 22.095022830973036 26.892958998919735 28.73157504129186 22.280443128815374 44-45 22.2768194939477 27.161751052669658 28.42317962699622 22.138249826386424 46-47 22.267627048330922 27.40818666314142 27.996073317458453 22.3281129710692 48-49 22.314033240626348 27.346582114652367 27.964222948460733 22.375161696260548 50-51 22.30996135672565 27.432413996780262 27.929625591824987 22.3279990546691 52-53 22.423142988856437 27.530748805708726 27.68962136837658 22.356486837058252 54-55 22.315599391152233 27.344729957352254 27.846518836481373 22.49315181501414 56-57 22.323392598613363 27.677080748239657 27.741303773047765 22.25822288009921 58-59 22.408542667015585 27.329008377404474 27.79213569380178 22.47031326177817 60-61 22.394511579535305 27.38384795305029 27.976669851554476 22.24497061585993 62-63 22.362764130995586 27.35779746412606 27.95181961228217 22.327618792596184 64-65 22.246772145307894 27.26654770003097 27.92922657261135 22.55745358204979 66-67 22.208777172130674 27.50814986418583 27.818304517198733 22.464768446484758 68-69 22.14111375092597 27.333765200583294 27.913607841771594 22.611513206719142 70-71 22.066302466969468 27.27401665743658 27.968166674442934 22.691514201151023 72-73 22.330555171899054 27.27361556965045 27.75567033506145 22.64015892338905 74-75 22.202142232484242 27.47620507128124 27.67579152868248 22.645861167552038 76-77 22.29686530603965 27.313220892550138 27.846218605303775 22.54369519610644 78-79 22.286563116033207 27.343307693335007 27.769976064037955 22.600153126593835 80-81 22.03277883726399 27.530010526865656 27.840998515554727 22.596212120315634 82-83 22.357880600754196 27.528695711267698 27.625070768300734 22.488352919677364 84-85 22.224121209723783 27.324542429945538 27.765592138394602 22.685744221936076 86-87 22.128119606248763 27.186264350231003 28.043821138348825 22.64179490517141 88-89 22.300368054020026 27.17686405532914 27.866027119556357 22.656740771094473 90-91 22.146110616360314 27.447900712644003 27.963078292115746 22.442910378879937 92-93 22.209035424641087 27.28835316678408 27.974015922550894 22.52859548602394 94-95 22.05104181318503 27.222728649794963 27.8796944483516 22.846535088668404 96-97 22.20665737171213 27.22339691197198 27.84526960693568 22.724676109380212 98-99 22.056027716327566 27.217490471413562 27.70415586005107 23.022325952207794 100-101 21.940406068836246 27.22045386272207 27.930042274395262 22.909097794046424 102-103 22.086796733289045 27.113362849361756 27.78692335130329 23.01291706604592 104-105 22.06244542850405 26.906086734551565 28.031301322563714 23.00016651438067 106-107 21.913175136750038 27.12049624254375 27.965289935778703 23.001038684927504 108-109 21.941123008321735 27.118438332190497 27.717209750212675 23.22322890927509 110-111 21.86619774263252 27.18282458659503 27.84339010167722 23.107587569095237 112-113 21.963163881960913 27.020721267332597 27.865270428336903 23.15084442236959 114-115 21.812314541188023 27.071099370000017 27.74285479064212 23.373731298169844 116-117 21.7088692656266 26.96818343570883 27.9028082190044 23.42013907966017 118-119 21.64836093703246 26.998666164012803 27.755436353708284 23.597536545246456 120-121 21.587901758851785 27.07312326840652 27.82031108535988 23.51866388738182 122-123 21.63362237578765 27.05892456191422 27.68474931678262 23.622703745515512 124-125 21.535120284116115 26.9510836930767 27.72743962803077 23.786356394776416 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 186.0 1 147.5 2 241.0 3 402.0 4 323.5 5 277.5 6 594.0 7 1417.5 8 2310.0 9 2628.5 10 2211.5 11 1617.0 12 1449.0 13 1623.5 14 1910.5 15 2131.0 16 2317.0 17 2529.5 18 2796.0 19 3057.0 20 3425.5 21 4033.5 22 4821.0 23 6080.0 24 7696.5 25 10032.0 26 13881.5 27 18226.0 28 22073.5 29 26956.5 30 33146.0 31 39056.5 32 45420.0 33 52844.0 34 61260.5 35 68642.5 36 76820.5 37 85539.0 38 90817.0 39 94169.5 40 97133.0 41 101082.5 42 104753.5 43 108297.5 44 114078.5 45 118408.0 46 118131.0 47 116837.0 48 118550.0 49 118102.0 50 114528.0 51 109310.0 52 102516.0 53 98092.5 54 94290.0 55 86357.0 56 75775.5 57 67428.0 58 60879.0 59 53329.5 60 44267.0 61 35237.0 62 27035.5 63 20381.0 64 15355.0 65 11322.5 66 8225.0 67 6198.5 68 4453.0 69 3122.5 70 2345.5 71 1727.5 72 1323.5 73 1101.5 74 837.5 75 608.5 76 449.5 77 284.0 78 167.0 79 106.0 80 76.0 81 62.0 82 45.5 83 26.0 84 16.0 85 15.0 86 13.5 87 7.5 88 3.5 89 1.0 90 0.5 91 1.5 92 1.5 93 2.0 94 1.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05798389163537565 2 0.07603835118831477 3 0.0 4 0.00787769489856707 5 0.03245745537607034 6 4.7333789090102575E-4 7 3.3809849350073266E-5 8 0.0 9 4.0571819220087913E-4 10-11 0.06324132320931204 12-13 0.010244384353072199 14-15 0.0072184028362406415 16-17 0.056546973037997536 18-19 1.1833447272525644E-4 20-21 0.010734627168648262 22-23 0.012154640841351339 24-25 0.0 26-27 0.0 28-29 0.05382528016531664 30-31 1.6904924675036633E-5 32-33 0.004209326244084121 34-35 0.0 36-37 4.564329662259891E-4 38-39 0.0 40-41 1.6904924675036633E-5 42-43 0.003820512976558279 44-45 0.0 46-47 1.6904924675036632E-4 48-49 4.0571819220087913E-4 50-51 7.945314597267218E-4 52-53 5.071477402510989E-5 54-55 0.0 56-57 0.001893351563604103 58-59 0.0 60-61 0.0 62-63 0.0 64-65 8.790560831019049E-4 66-67 0.0 68-69 6.42387137651392E-4 70-71 0.0 72-73 0.0022483549817798724 74-75 0.0034655095583825094 76-77 4.3952804155095247E-4 78-79 0.0011664398025775277 80-81 0.00160596784412848 82-83 8.452462337518316E-5 84-85 5.071477402510989E-5 86-87 4.3952804155095247E-4 88-89 0.0 90-91 0.0 92-93 0.007252212685590716 94-95 0.006153392581713334 96-97 0.006745064945339616 98-99 0.0013185841246528573 100-101 5.916723636262822E-4 102-103 1.0142954805021978E-4 104-105 5.240526649261356E-4 106-107 0.004462900114209672 108-109 0.005477195594711869 110-111 8.452462337518316E-4 112-113 0.0035331292570826567 114-115 6.592920623264286E-4 116-117 0.0020454958856794326 118-119 0.002806217496056081 120-121 0.012256070389401557 122-123 7.100068363515386E-4 124-125 0.0018257318649039563 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2957718.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.88500875209286 #Duplication Level Percentage of deduplicated Percentage of total 1 75.84448967932842 40.86880990168628 2 12.673019094880008 13.657714896860984 3 4.622251310206113 7.47210156914487 4 2.23568850546083 4.8188037873484095 5 1.2435349906434467 3.3503946927177917 6 0.7689506548735388 2.48609476606729 7 0.5073775012668911 1.9138028767467001 8 0.3595172896166824 1.5498073838018906 9 0.2530949736016214 1.2274222380870674 >10 1.3393525493810912 13.009793412318032 >50 0.10167944140277457 3.762372159581321 >100 0.04890105428467727 4.656251779087209 >500 0.0017647865150987026 0.5570221068557173 >1k 2.521123592152917E-4 0.25872778542443586 >5k 1.2605617960764585E-4 0.4108806442720957 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6538 0.221048795050779 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5560 0.18798276238640735 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3594 0.12151259856416333 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.3809849350073266E-5 0.0 10-11 0.0 0.0 0.0 5.07147740251099E-5 0.0 12-13 0.0 0.0 0.0 6.761969870014653E-5 0.0 14-15 0.0 0.0 0.0 6.761969870014653E-5 0.0 16-17 0.0 0.0 0.0 6.761969870014653E-5 1.6904924675036633E-5 18-19 0.0 0.0 0.0 6.761969870014653E-5 3.3809849350073266E-5 20-21 0.0 0.0 0.0 1.1833447272525642E-4 3.3809849350073266E-5 22-23 0.0 0.0 0.0 2.0285909610043962E-4 3.3809849350073266E-5 24-25 0.0 0.0 0.0 4.0571819220087924E-4 3.3809849350073266E-5 26-27 0.0 0.0 0.0 6.931019116765019E-4 3.3809849350073266E-5 28-29 0.0 0.0 0.0 0.001200249651927601 3.3809849350073266E-5 30-31 0.0 0.0 0.0 0.003042886441506594 3.3809849350073266E-5 32-33 0.0 0.0 0.0 0.00693101911676502 3.3809849350073266E-5 34-35 0.0 0.0 0.0 0.011174155210199214 3.3809849350073266E-5 36-37 0.0 0.0 0.0 0.018663036841240443 3.3809849350073266E-5 38-39 0.0 0.0 0.0 0.03353937055527268 3.3809849350073266E-5 40-41 0.0 0.0 0.0 0.05091763312121034 3.3809849350073266E-5 42-43 0.0 0.0 0.0 0.06751826915209631 3.3809849350073266E-5 44-45 0.0 0.0 0.0 0.08728012609721414 3.3809849350073266E-5 46-47 0.0 0.0 0.0 0.11028772857993899 3.3809849350073266E-5 48-49 0.0 0.0 0.0 0.13620297810677015 3.3809849350073266E-5 50-51 0.0 0.0 0.0 0.16656422282313593 3.3809849350073266E-5 52-53 0.0 0.0 0.0 0.19574212281224918 3.3809849350073266E-5 54-55 0.0 0.0 0.0 0.2260357478299148 3.3809849350073266E-5 56-57 0.0 0.0 0.0 0.26398730372537205 3.3809849350073266E-5 58-59 0.0 0.0 0.0 0.30526912978181153 3.3809849350073266E-5 60-61 0.0 0.0 0.0 0.3541074571679923 3.3809849350073266E-5 62-63 0.0 0.0 0.0 0.4015595807308202 3.3809849350073266E-5 64-65 0.0 0.0 0.0 0.45127696420010294 3.3809849350073266E-5 66-67 0.0 0.0 0.0 0.5096834789523544 3.3809849350073266E-5 68-69 0.0 0.0 0.0 0.576880554535625 3.3809849350073266E-5 70-71 0.0 0.0 0.0 0.6540853455265174 6.761969870014653E-5 72-73 0.0 0.0 0.0 0.7339273047667154 6.761969870014653E-5 74-75 0.0 0.0 0.0 0.8184181182925485 6.761969870014653E-5 76-77 0.0 0.0 0.0 0.9098737607844967 6.761969870014653E-5 78-79 0.0 0.0 0.0 1.0211757848449379 6.761969870014653E-5 80-81 0.0 0.0 0.0 1.1472696179960362 6.761969870014653E-5 82-83 0.0 0.0 0.0 1.2789589812145716 6.761969870014653E-5 84-85 0.0 0.0 0.0 1.4137250407239637 6.761969870014653E-5 86-87 0.0 0.0 0.0 1.5566899887007484 6.761969870014653E-5 88-89 0.0 0.0 0.0 1.7216651486044308 6.761969870014653E-5 90-91 0.0 0.0 0.0 1.9151927262842503 6.761969870014653E-5 92-93 0.0 0.0 0.0 2.126893097989734 6.761969870014653E-5 94-95 0.0 0.0 0.0 2.3389822829627436 6.761969870014653E-5 96-97 3.3809849350073266E-5 0.0 0.0 2.5565317586057903 6.761969870014653E-5 98-99 3.3809849350073266E-5 0.0 0.0 2.8024815077028977 6.761969870014653E-5 100-101 3.3809849350073266E-5 0.0 0.0 3.0869744850590894 6.761969870014653E-5 102-103 3.3809849350073266E-5 0.0 0.0 3.395962698269409 6.761969870014653E-5 104-105 3.3809849350073266E-5 0.0 0.0 3.702330648155098 6.761969870014653E-5 106-107 3.3809849350073266E-5 0.0 0.0 4.0152746137393756 6.761969870014653E-5 108-109 3.3809849350073266E-5 0.0 0.0 4.353119533369983 6.761969870014653E-5 110-111 3.3809849350073266E-5 0.0 0.0 4.745347595680183 6.761969870014653E-5 112-113 3.3809849350073266E-5 0.0 0.0 5.167294515569098 6.761969870014653E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10630 0.0 40.580822 1 GGTATCA 7275 0.0 39.80328 1 ATCAACG 12445 0.0 33.851715 3 TCAACGC 12645 0.0 33.318554 4 CAACGCA 12615 0.0 32.865074 5 TATCAAC 13355 0.0 31.969326 2 AACGCAG 13110 0.0 31.61347 6 GTACATG 12750 0.0 28.739586 1 TACATGG 12950 0.0 27.681206 2 ACGCAGA 15230 0.0 27.017868 7 CGCAGAG 15350 0.0 26.76786 8 ACATGGG 12950 0.0 26.14477 3 GAGTACT 8685 0.0 23.967558 12-13 GCAGAGT 17305 0.0 22.746267 9 GTACTTT 9125 0.0 22.648687 14-15 AGTACTT 9020 0.0 22.285053 12-13 CATGGGA 8985 0.0 21.259775 4 AGTACAT 9825 0.0 20.30348 2 CGTAGAC 270 1.1059492E-6 19.834492 3 CAGAGTA 16710 0.0 19.665411 10-11 >>END_MODULE