FastQCFastQC Report
Thu 26 May 2016
SRR1364617_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364617_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2776036
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75720.27276303333242075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36830.1326711901430673No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG905.1595507E-639.6650669
GTATCAA141000.033.4622081
GTACATG161450.032.6922041
TACATGG161400.031.558122
CGCATAG3900.028.9865328
TATCAAC165650.028.3748862
ATCAACG160500.028.2089613
ACATGGG170650.028.0322383
TCAACGC161300.027.7735884
CAACGCA162100.027.4900135
AACGCAG161950.027.0133426
CATGGGA130900.024.3934754
ACGCAGA181000.023.770897
CGCAGAT11550.023.6964428
GAGTACT52950.023.36994212-13
ATGGGAG52450.023.1708015
CGCAGAG174850.022.6969498
GGTATCA98750.022.200321
GTATAGG9450.022.0640261
ACGCATA5400.022.0405247
Produced by FastQC (version 0.11.5)