##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364617_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2776036 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.985443272349492 31.0 27.0 31.0 10.0 34.0 2 26.645129962291556 31.0 26.0 31.0 10.0 34.0 3 27.098867233710227 31.0 27.0 33.0 10.0 34.0 4 30.319663001488454 35.0 30.0 37.0 10.0 37.0 5 30.7131427690419 35.0 31.0 37.0 10.0 37.0 6 30.76566982560745 35.0 31.0 37.0 10.0 37.0 7 30.536619481879917 35.0 31.0 37.0 8.0 37.0 8 30.35997407814596 35.0 30.0 37.0 8.0 37.0 9 31.24780334260795 37.0 30.0 39.0 8.0 39.0 10-11 31.217303197797147 37.0 30.0 39.0 8.0 39.0 12-13 31.241064236919115 37.0 30.0 39.0 7.0 39.0 14-15 31.94383051948894 38.0 30.5 40.0 7.0 41.0 16-17 32.38833808351188 38.0 31.0 40.0 7.0 41.0 18-19 32.23702106168652 38.0 30.5 40.0 4.5 41.0 20-21 32.14089244519884 38.0 30.0 40.0 2.0 41.0 22-23 32.241103501539605 38.0 30.5 40.0 2.0 41.0 24-25 32.360002896216045 38.0 30.5 40.0 2.0 41.0 26-27 32.172660044754466 38.0 30.0 40.0 2.0 41.0 28-29 32.076898678547394 38.0 30.0 40.0 2.0 41.0 30-31 31.962525882229194 38.0 30.0 40.0 2.0 41.0 32-33 31.84834904878755 38.0 30.0 40.0 2.0 41.0 34-35 31.90317560723276 38.0 30.0 40.0 2.0 41.0 36-37 31.85570684241847 38.0 30.0 40.0 2.0 41.0 38-39 31.572834970439864 38.0 30.0 40.0 2.0 41.0 40-41 31.434787048871126 37.5 30.0 40.0 2.0 41.0 42-43 30.963483722833566 37.0 28.5 40.0 2.0 41.0 44-45 30.873578008354357 37.0 29.0 40.0 2.0 41.0 46-47 30.84078070313209 37.0 29.0 40.0 2.0 41.0 48-49 30.774288589917422 37.0 29.0 40.0 2.0 41.0 50-51 30.552155123348545 37.0 28.0 40.0 2.0 41.0 52-53 30.45731791662644 37.0 28.0 40.0 2.0 41.0 54-55 30.301664855931264 36.0 28.0 40.0 2.0 41.0 56-57 30.09389377515277 36.0 28.0 40.0 2.0 41.0 58-59 29.888852305949925 36.0 28.0 40.0 2.0 41.0 60-61 29.591662536076623 35.0 27.0 39.5 2.0 41.0 62-63 29.422328096609697 35.0 26.5 39.0 2.0 41.0 64-65 29.204071921257505 35.0 26.5 39.0 2.0 40.5 66-67 28.942981106873255 35.0 26.0 39.0 2.0 40.0 68-69 28.705356846957315 34.0 26.0 38.5 2.0 40.0 70-71 28.27127818227141 34.0 26.0 38.0 2.0 40.0 72-73 28.035695142282016 34.0 26.0 37.0 2.0 39.5 74-75 27.997104504408444 34.0 26.0 37.0 2.0 39.0 76-77 27.72412839747035 34.0 26.0 36.5 2.0 39.0 78-79 27.413113158474893 34.0 26.0 36.0 2.0 39.0 80-81 26.99003291023603 33.5 25.0 35.5 2.0 37.5 82-83 27.061229753504637 34.0 26.0 35.0 2.0 37.0 84-85 26.86573246888729 34.0 25.0 35.0 2.0 37.0 86-87 26.707476776237772 34.0 25.0 35.0 2.0 36.0 88-89 26.515019077562393 33.5 25.0 35.0 2.0 36.0 90-91 26.35826876884882 33.0 25.0 35.0 2.0 36.0 92-93 26.221818629153226 33.0 24.0 35.0 2.0 35.5 94-95 26.104053405647477 33.0 24.0 35.0 2.0 35.0 96-97 25.98212919428999 33.0 24.0 35.0 2.0 35.0 98-99 25.881707946150556 33.0 23.0 35.0 2.0 35.0 100-101 25.768792263500906 33.0 23.0 35.0 2.0 35.0 102-103 24.66966152456236 31.5 19.5 34.0 2.0 35.0 104-105 25.48052276699582 33.0 20.0 35.0 2.0 35.0 106-107 25.624625184975987 33.0 21.0 35.0 2.0 35.0 108-109 25.580207713444636 33.0 20.0 35.0 2.0 35.0 110-111 25.507940819211278 33.0 19.5 35.0 2.0 35.0 112-113 25.409919756083855 33.0 19.0 35.0 2.0 35.0 114-115 25.29868290612946 33.0 18.5 35.0 2.0 35.0 116-117 25.18705953381008 33.0 17.5 35.0 2.0 35.0 118-119 25.063414163216905 33.0 13.0 35.0 2.0 35.0 120-121 24.878941411422616 33.0 2.0 35.0 2.0 35.0 122-123 24.671637543605343 33.0 2.0 35.0 2.0 35.0 124-125 23.762154741509114 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5498305312996337 1101 2 1.860109308175879 1101 3 1.398890769980298 1101 4 1.4519552621378367 1101 5 1.3173505508463066 1101 6 1.3277661282888893 1101 7 1.2678158392126484 1101 8 1.56333615675862 1101 9 1.8464964497906138 1101 10-11 1.847044144518474 1101 12-13 1.908303792774003 1101 14-15 2.1949086311652124 1101 16-17 1.8406524581807702 1101 18-19 1.839794034871069 1101 20-21 1.9990808632778325 1101 22-23 1.732406110017216 1101 24-25 1.7305824046804474 1101 26-27 1.9269227267144018 1101 28-29 1.898381834147493 1101 30-31 2.0380451851910415 1101 32-33 2.057435345248166 1101 34-35 1.8314024889991032 1101 36-37 1.8203421522189416 1101 38-39 1.7874308889340433 1101 40-41 1.8540111771726409 1101 42-43 1.980763889400837 1101 44-45 1.8361778769032995 1101 46-47 1.6040200857902178 1101 48-49 1.4362932234114858 1101 50-51 1.5288141785516451 1101 52-53 1.4882408373808715 1101 54-55 1.4616203891354864 1101 56-57 1.5304841977670307 1101 58-59 1.4171926384822058 1101 60-61 1.5632607385696744 1101 62-63 1.377706321324947 1101 64-65 1.2924567410965118 1101 66-67 1.2886446388727428 1101 68-69 1.1266581372056024 1101 70-71 1.0393785029812328 1101 72-73 1.0269569196386286 1101 74-75 0.9725186818491345 1101 76-77 1.097188582350661 1101 78-79 1.0774772502779228 1101 80-81 1.2484070311299469 1101 82-83 1.2242021821395532 1101 84-85 1.2322136214978698 1101 86-87 1.1844055806957137 1101 88-89 1.146665570185963 1101 90-91 1.1896904592366901 1101 92-93 1.2199709265696157 1101 94-95 1.155649157218992 1101 96-97 1.1350130123616644 1101 98-99 1.199980318065247 1101 100-101 1.1271029056598358 1101 102-103 0.9299088433898461 1101 104-105 1.0247354764020251 1101 106-107 1.088334607295021 1101 108-109 0.982905679226306 1101 110-111 1.083768894054355 1101 112-113 0.9736606120596356 1101 114-115 0.9308099644964507 1101 116-117 0.9540308078660296 1101 118-119 0.9949409347810096 1101 120-121 1.0028742508738766 1101 122-123 0.9011479699999718 1101 124-125 0.7899904607923744 1102 1 0.7438713824112213 1102 2 1.2185961663555105 1102 3 0.8052516027612562 1102 4 0.6570250015226158 1102 5 0.6830148782537542 1102 6 0.3067400924857111 1102 7 0.5227101049295833 1102 8 0.48534372281843474 1102 9 1.1744159937695073 1102 10-11 1.131920237429501 1102 12-13 1.2484795470126748 1102 14-15 1.4334470208523626 1102 16-17 1.0977496724632374 1102 18-19 0.9502184683810455 1102 20-21 1.272525974857345 1102 22-23 1.255556563782065 1102 24-25 0.8832014456192354 1102 26-27 1.2300825094590593 1102 28-29 0.9136546191285362 1102 30-31 0.9530773222405351 1102 32-33 1.2014144707790564 1102 34-35 1.0588971435404346 1102 36-37 0.9290804427710668 1102 38-39 1.165442729022704 1102 40-41 1.2295978624505075 1102 42-43 1.4692141330882897 1102 44-45 1.1340809899534676 1102 46-47 0.9719895363039583 1102 48-49 0.8895939053154898 1102 50-51 0.9958390990858028 1102 52-53 0.9045548908225989 1102 54-55 0.8659837914775501 1102 56-57 0.987136502714776 1102 58-59 1.098163916213423 1102 60-61 1.1753770141323052 1102 62-63 0.883929908212469 1102 64-65 0.6851891384923441 1102 66-67 0.7439881101650876 1102 68-69 0.7072221780800945 1102 70-71 0.9490744752701836 1102 72-73 0.6690705414689084 1102 74-75 0.71969649010002 1102 76-77 0.5720460465282002 1102 78-79 0.6420268055147176 1102 80-81 0.7468179773667316 1102 82-83 0.7072311253578683 1102 84-85 0.6286354959681475 1102 86-87 0.6286354663981761 1102 88-89 0.5863717774646666 1102 90-91 0.6693253723006549 1102 92-93 0.6828206902471514 1102 94-95 0.7809467668724999 1102 96-97 0.5794564826018664 1102 98-99 0.7669530407361869 1102 100-101 0.8053335909891395 1102 102-103 0.7260647893285253 1102 104-105 0.8195155439494464 1102 106-107 0.7294495723581065 1102 108-109 0.8485389392605143 1102 110-111 0.8765076042936677 1102 112-113 0.7830879377871653 1102 114-115 0.8041640951419176 1102 116-117 0.9147644830281472 1102 118-119 0.8449463123884158 1102 120-121 0.8858102842337487 1102 122-123 0.7209388512144699 1102 124-125 0.7062096169990113 1103 1 1.0416001940469783 1103 2 1.3872997687324577 1103 3 0.9859921183072267 1103 4 0.9184638412633994 1103 5 0.9000158464497048 1103 6 0.9437117707694576 1103 7 0.8560190741051983 1103 8 1.0055373855304843 1103 9 1.5772889454676147 1103 10-11 1.535365139200099 1103 12-13 1.4835819573295872 1103 14-15 1.7989720120980266 1103 16-17 1.4844750679226166 1103 18-19 1.4536089771205312 1103 20-21 1.7984450774252494 1103 22-23 1.6005049653766932 1103 24-25 1.371981133556126 1103 26-27 1.6713405938070807 1103 28-29 1.6865089390083696 1103 30-31 1.6636341275290683 1103 32-33 1.7574016881227266 1103 34-35 1.601613225736834 1103 36-37 1.5842199520753653 1103 38-39 1.5868566185331083 1103 40-41 1.5274328140010134 1103 42-43 1.7049650259425455 1103 44-45 1.5262885267106654 1103 46-47 1.3996453936542608 1103 48-49 1.191820609000775 1103 50-51 1.1774138861223875 1103 52-53 1.1637597352717606 1103 54-55 1.1332178705638434 1103 56-57 1.1765003424041112 1103 58-59 1.2436870022329103 1103 60-61 1.2669247193197286 1103 62-63 1.094764323771109 1103 64-65 0.984040004855725 1103 66-67 0.9827528179671923 1103 68-69 1.0248717170183035 1103 70-71 1.0656867832076564 1103 72-73 0.9359339540994398 1103 74-75 0.9053925047961329 1103 76-77 0.946078872281447 1103 78-79 0.931133998302144 1103 80-81 0.9977562865549494 1103 82-83 0.9555090272922264 1103 84-85 0.9947333781387258 1103 86-87 0.9479419052687881 1103 88-89 1.0047682650273337 1103 90-91 1.0223580778012007 1103 92-93 0.8589834294919605 1103 94-95 0.9309606003433828 1103 96-97 0.9060082790272723 1103 98-99 0.9142362558406312 1103 100-101 0.907990029021807 1103 102-103 0.855439642871616 1103 104-105 0.8552731403772675 1103 106-107 0.9489673796074349 1103 108-109 1.033313447804776 1103 110-111 0.991740975388506 1103 112-113 1.0096812787276832 1103 114-115 1.0309123239137143 1103 116-117 1.0918571666950356 1103 118-119 1.1391279339534606 1103 120-121 1.142409878902761 1103 122-123 0.9896102293956872 1103 124-125 0.9117202190828237 1104 1 1.2437909719670834 1104 2 1.6788546454817102 1104 3 1.0801758101906245 1104 4 1.236011342766215 1104 5 0.7901866025430699 1104 6 0.904359386584666 1104 7 0.6621984764426543 1104 8 1.0083665987633594 1104 9 1.3311347604884602 1104 10-11 1.4738449033114982 1104 12-13 1.4538035419473623 1104 14-15 1.5171723297279343 1104 16-17 1.3030101335778426 1104 18-19 1.323397134124427 1104 20-21 1.6433324020093423 1104 22-23 1.5042937892360797 1104 24-25 1.3848945015843839 1104 26-27 1.636120716164072 1104 28-29 1.5459855911843832 1104 30-31 1.6154933414624857 1104 32-33 1.6238965988111573 1104 34-35 1.6061167197283837 1104 36-37 1.4337913986868358 1104 38-39 1.520657069397533 1104 40-41 1.5526608711234111 1104 42-43 1.725696105113112 1104 44-45 1.5323805685888274 1104 46-47 1.3630988901583336 1104 48-49 1.3494408499940853 1104 50-51 1.3680799522890403 1104 52-53 1.1545700420873146 1104 54-55 1.177513396468857 1104 56-57 1.1392020188132506 1104 58-59 1.1169512814795297 1104 60-61 1.1329347114532418 1104 62-63 1.0226462756670287 1104 64-65 0.9454440219117011 1104 66-67 0.9796715278960413 1104 68-69 0.8541948973942901 1104 70-71 0.912217117250588 1104 72-73 0.8184200230056788 1104 74-75 0.7193628143046062 1104 76-77 0.7699467046875377 1104 78-79 0.8076311437930173 1104 80-81 0.9890386221175653 1104 82-83 0.9508148039805455 1104 84-85 0.948406925003539 1104 86-87 0.8991664740051242 1104 88-89 0.8856346285644179 1104 90-91 0.8990006248773099 1104 92-93 0.9394791128859517 1104 94-95 0.892820341819931 1104 96-97 0.8936431119659751 1104 98-99 0.9440621330318955 1104 100-101 0.8832887183076075 1104 102-103 0.7973715706866429 1104 104-105 0.9624996674538586 1104 106-107 0.9716146866363431 1104 108-109 0.9504634253571638 1104 110-111 0.91422332133126 1104 112-113 0.9077664337493871 1104 114-115 0.972691395111152 1104 116-117 0.9308993475526428 1104 118-119 0.8876273526032108 1104 120-121 0.967169299033273 1104 122-123 0.9963874228924183 1104 124-125 0.7145759858889562 1105 1 1.0827417814527251 1105 2 1.3353693165291638 1105 3 0.8773576190554451 1105 4 0.9533149141530579 1105 5 0.80171416012967 1105 6 0.6833535432699804 1105 7 0.6024959267651298 1105 8 0.7291711959656766 1105 9 1.012957312493917 1105 10-11 1.0309462482275187 1105 12-13 1.0418941174978933 1105 14-15 1.3396469135735671 1105 16-17 0.8373988489288138 1105 18-19 0.9399883766191479 1105 20-21 0.9686888891613634 1105 22-23 1.0229867822548329 1105 24-25 0.7141896984177691 1105 26-27 1.0791227206131921 1105 28-29 1.1316075774306498 1105 30-31 1.0809491430423925 1105 32-33 1.100134340296755 1105 34-35 1.0137922420029426 1105 36-37 0.7841596284410173 1105 38-39 1.0066356624195798 1105 40-41 1.1426978921735191 1105 42-43 1.3240757428567527 1105 44-45 1.0980130628087679 1105 46-47 0.7287906732523055 1105 48-49 0.8560608567175017 1105 50-51 1.1112853783337933 1105 52-53 0.8731120254092062 1105 54-55 0.9770950527048861 1105 56-57 0.9482102221099922 1105 58-59 0.9843723233991426 1105 60-61 0.8706612023519931 1105 62-63 0.7920522819370959 1105 64-65 0.5548871883292712 1105 66-67 0.5143603319688985 1105 68-69 0.45555978780947015 1105 70-71 0.6652921155619431 1105 72-73 0.5533073413180531 1105 74-75 0.583450943521413 1105 76-77 0.6037633180131365 1105 78-79 0.6826523283264123 1105 80-81 0.7514096941322919 1105 82-83 0.7240365856534972 1105 84-85 0.654257940119642 1105 86-87 0.721351373614084 1105 88-89 0.8132019130349519 1105 90-91 0.6051890902758288 1105 92-93 0.6138682165968596 1105 94-95 0.49478952696179235 1105 96-97 0.545715401661564 1105 98-99 0.601494429600006 1105 100-101 0.6609374137020048 1105 102-103 0.5257620112904391 1105 104-105 0.5937868558381361 1105 106-107 0.6540274567120186 1105 108-109 0.6999857370802118 1105 110-111 0.6840070883090696 1105 112-113 0.6545269110381753 1105 114-115 0.7516335571898871 1105 116-117 0.6915932388067425 1105 118-119 0.6920284554118759 1105 120-121 0.5899848439795932 1105 122-123 0.5842500178110903 1105 124-125 0.5422378176414213 1106 1 0.6325809274834633 1106 2 -1.5039869658285916 1106 3 0.09451353894509751 1106 4 0.5649150087936192 1106 5 0.6809292513633487 1106 6 0.6949111502457441 1106 7 0.7579505950726997 1106 8 0.7125033703457255 1106 9 1.2139989709604393 1106 10-11 1.3801645123691593 1106 12-13 1.4429652090044662 1106 14-15 1.508834959648297 1106 16-17 1.1321820612499351 1106 18-19 1.1622693300747073 1106 20-21 1.4377740835987325 1106 22-23 1.2226182351099268 1106 24-25 1.2155504298441215 1106 26-27 1.2748021427634555 1106 28-29 1.3811835207967604 1106 30-31 1.5497836318302838 1106 32-33 1.4993937472800383 1106 34-35 1.3543091176748092 1106 36-37 1.103582242287402 1106 38-39 1.4282408914418703 1106 40-41 1.4019947748953179 1106 42-43 1.7475960328487297 1106 44-45 1.5385474430486639 1106 46-47 1.245897917103946 1106 48-49 1.0799418763864672 1106 50-51 1.0482925498492612 1106 52-53 0.9965090071449723 1106 54-55 0.9457226792647724 1106 56-57 0.9929614538805325 1106 58-59 0.9679591941640453 1106 60-61 1.0846840123020236 1106 62-63 0.896201236298829 1106 64-65 0.8647509522771664 1106 66-67 0.7386230955433035 1106 68-69 0.6898898100351332 1106 70-71 0.788141929211001 1106 72-73 0.5985471374997147 1106 74-75 0.6376182880664167 1106 76-77 0.55375776378418 1106 78-79 0.6670164631393547 1106 80-81 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228974.0 3 30151.0 4 18270.0 5 15044.0 6 14514.0 7 15873.0 8 17337.0 9 18387.0 10 20486.0 11 22063.0 12 23608.0 13 24718.0 14 25761.0 15 22070.0 16 17644.0 17 15687.0 18 14042.0 19 12905.0 20 12552.0 21 13281.0 22 16242.0 23 23444.0 24 35551.0 25 45629.0 26 50712.0 27 51847.0 28 60124.0 29 67890.0 30 78599.0 31 91494.0 32 110447.0 33 138615.0 34 182253.0 35 249478.0 36 368452.0 37 472783.0 38 148694.0 39 415.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.11572976847838 19.39433031553673 14.016258707056679 22.473681208928213 2 12.855139383767906 21.114102451149606 43.08627336001468 22.94448480506782 3 18.97478229612784 27.571235813799746 28.124865656706145 25.329116233366268 4 11.604906253171881 17.874224254795163 38.18315334804424 32.337716143988715 5 12.629596560978 40.65883995518026 33.296682132919194 13.414881350922537 6 30.16759433657431 38.40640065158928 18.047485001857506 13.378520009978907 7 27.27643573678123 34.94456469786388 20.427020774881267 17.35197879047362 8 24.815510740909442 37.94447600574889 19.66035063606656 17.579662617275112 9 25.57958735091414 18.682215448805056 20.09214494150258 35.64605225877822 10-11 23.283733864420824 29.767869868173836 27.17066590529056 19.77773036211478 12-13 24.46669634977186 26.858002423238265 27.566211485629793 21.109089741360087 14-15 21.63163833978758 27.85738077684107 26.01145128386676 24.49952959950459 16-17 21.063679141047302 31.18332387780633 26.27659987065921 21.476397110487156 18-19 21.161204086276094 29.75608251005339 28.480352699966673 20.602360703703845 20-21 22.487155823430125 28.522687394168045 28.350974623786286 20.639182158615544 22-23 21.767093233918278 28.64638121653147 28.08309299786888 21.503432551681374 24-25 21.451746378095667 28.378558340782917 28.54727919132367 21.622416089797746 26-27 21.711726561672048 28.376964541793328 28.476930473577095 21.434378422957536 28-29 21.356513073017407 28.534381991578666 28.602993164681788 21.506111770722136 30-31 21.63591108090358 28.429774069418283 28.634468587021594 21.299846262656544 32-33 21.394544381709224 28.309108395993366 28.50072449253944 21.79562272975797 34-35 21.746827036107295 28.15902527241863 28.513501837769432 21.58064585370464 36-37 21.731448731382173 28.193757985060103 28.44451795805407 21.630275325503654 38-39 21.650857495163965 28.124405137483045 28.69805333468537 21.526684032667617 40-41 21.847640617739017 28.19748519041539 28.61994071654478 21.334933475300815 42-43 21.482070058374454 28.18941675206884 28.634525352030018 21.69398783752669 44-45 21.74565970092384 28.314272436124106 28.296249496656646 21.64381836629541 46-47 21.695611477582435 28.431604749959007 27.85314746746358 22.019636304994975 48-49 21.71883593823424 28.37946680390628 27.968289135431938 21.933408122427547 50-51 21.867935215952837 28.527873087344453 27.88212521692698 21.72206647977573 52-53 21.80414647929611 28.6726459535163 27.774796634720524 21.748410932467067 54-55 21.775932313408777 28.526153630218083 27.783680160722817 21.91423389565032 56-57 21.83865847060439 28.750383141797016 27.527811268545282 21.88314711905332 58-59 21.912317470853054 28.468432558005308 27.704136846358075 21.915113124783563 60-61 21.90378608788356 28.40268526594253 27.859192201436432 21.834336444737477 62-63 21.931943886055006 28.367890950916617 27.865403585247982 21.834761577780387 64-65 21.824371717139655 28.412592115778523 27.799860655130104 21.963175511951718 66-67 21.79704649050735 28.53910420888087 27.693371411176503 21.970477889435276 68-69 21.85385293895399 28.52992876940798 27.640759184620094 21.975459107017937 70-71 21.68673981755441 28.39722944709774 27.755701379613573 22.160329355734273 72-73 21.805701027546633 28.439884943049403 27.515671758451806 22.23874227095216 74-75 21.76550815377808 28.545417631172494 27.498378115613757 22.190696099435666 76-77 21.905359624230258 28.42502594733705 27.617957811568676 22.05165661686401 78-79 21.83322716343119 28.431644498439194 27.621699204194663 22.113429133934957 80-81 21.590046368821795 28.62343563622616 27.721997024906265 22.06452097004578 82-83 21.68224669933288 28.700052579791862 27.597139587171977 22.020561133703286 84-85 21.898619320068384 28.408319681880723 27.56207089905885 22.130990098992044 86-87 21.790653929782614 28.402778792521598 27.73384162527865 22.072725652417137 88-89 22.034663591359337 28.204147155115233 27.710290463931614 22.050898789593816 90-91 21.854448335863648 28.48446106830189 27.71820601586513 21.942884579969327 92-93 21.80744442779008 28.428274922516632 27.74520557285024 22.01907507684305 94-95 21.650093270455457 28.566196766383666 27.629451093447255 22.154258869713622 96-97 21.750934860136265 28.46571097173142 27.48524878193091 22.29810538620141 98-99 21.725038204069616 28.410858613917394 27.443617431156913 22.420485750856074 100-101 21.77711907521623 28.509765808515812 27.51788262634522 22.195232489922734 102-103 21.613931221413818 28.380836848949503 27.72164979700113 22.28358213263555 104-105 21.464095701034896 28.329578067274507 27.861146647978703 22.345179583711896 106-107 21.36030750468776 28.600673680975607 27.743522218541074 22.295496595795562 108-109 21.38936040461912 28.545823843647128 27.536224041909197 22.52859170982456 110-111 21.33844690706089 28.640148358074708 27.600422829168576 22.42098190569582 112-113 21.48776558742166 28.602030251460803 27.475074442192938 22.4351297189246 114-115 21.304246540071272 28.576335522752334 27.5210834053964 22.598334531779994 116-117 21.164277422335285 28.551508828441424 27.725193686206527 22.559020063016764 118-119 21.130922171959895 28.68805953546601 27.58038732019189 22.600630972382202 120-121 21.109598405505736 28.699598780477253 27.58367994161982 22.60712287239719 122-123 21.118035046517473 28.554032722537166 27.424157782539062 22.903774448406303 124-125 21.1584757943282 28.545344420909064 27.46198150055168 22.83419828421106 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8897.0 1 6738.5 2 3737.5 3 2321.0 4 1455.5 5 1071.5 6 1030.5 7 1318.0 8 1798.0 9 1943.5 10 1741.5 11 1604.0 12 1663.0 13 1860.5 14 2009.5 15 2168.0 16 2397.5 17 2696.0 18 3078.0 19 3536.5 20 4156.5 21 5095.0 22 6530.0 23 8313.0 24 10368.5 25 13311.0 26 17454.0 27 22465.5 28 27793.5 29 33195.5 30 38810.5 31 44339.5 32 49790.0 33 56193.5 34 62949.5 35 69053.5 36 75019.5 37 81046.5 38 86211.5 39 89594.5 40 92482.5 41 95869.5 42 98886.0 43 101609.5 44 104783.5 45 107379.5 46 108879.0 47 110160.0 48 113892.5 49 112521.0 50 105403.0 51 98465.0 52 90622.0 53 83621.5 54 76770.0 55 68692.0 56 60350.5 57 53425.0 58 47645.0 59 41845.5 60 34982.0 61 27946.0 62 21809.5 63 16407.5 64 12107.0 65 9268.0 66 6925.5 67 5126.5 68 3980.0 69 3075.0 70 2347.5 71 1800.0 72 1444.0 73 1269.0 74 999.0 75 746.5 76 561.5 77 391.0 78 301.0 79 235.5 80 182.0 81 129.5 82 89.5 83 70.0 84 57.0 85 39.5 86 25.5 87 20.0 88 15.5 89 14.0 90 18.0 91 12.0 92 6.5 93 7.0 94 4.5 95 2.5 96 2.0 97 2.5 98 1.0 99 0.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20107808400179247 2 0.23630817467785 3 0.2860553681580498 4 0.2726549655696108 5 0.3063721075663284 6 0.22308788502742757 7 0.21469462211585155 8 0.1950262892844329 9 0.1945579956455896 10-11 0.1391372446178652 12-13 0.16347050254391515 14-15 0.17986077990343063 16-17 0.1544828669368841 18-19 0.17850993286830574 20-21 0.17429529011871603 22-23 0.19558463939228454 24-25 0.18684916189847683 26-27 0.23747890877495823 28-29 0.16647838860879327 30-31 0.14771062046745792 32-33 0.1848318969926903 34-35 0.14630573955092802 36-37 0.14634176213853134 38-39 0.17696096160136252 40-41 0.19626906855674783 42-43 0.2620102909328265 44-45 0.16455118017201506 46-47 0.15059242747572438 48-49 0.15278980531952757 50-51 0.15727461747614224 52-53 0.17701499548276753 54-55 0.16413692041457675 56-57 0.1636326041881301 58-59 0.13962354955051015 60-61 0.15286185049473422 62-63 0.14144269022447836 64-65 0.13607532467158207 66-67 0.12499837898355785 68-69 0.1282944457492626 70-71 0.12942915725876752 72-73 0.10714918682610745 74-75 0.1090403726752823 76-77 0.1294471685525692 78-79 0.13508470351249047 80-81 0.1449008586343981 82-83 0.14619767178811804 84-85 0.13481453410546548 86-87 0.15851739674845713 88-89 0.15419468623605748 90-91 0.14345995513026488 92-93 0.1437121132434882 94-95 0.16303823149267516 96-97 0.1524115681496926 98-99 0.14697215742158964 100-101 0.12905092008893257 102-103 0.165415722274495 104-105 0.1255387177976078 106-107 0.1316805689839757 108-109 0.1421991645641483 110-111 0.11833420027694166 112-113 0.1109135472306555 114-115 0.10813980798519904 116-117 0.11249854108520206 118-119 0.09717093005998481 120-121 0.08976828830750033 122-123 0.11552443844388186 124-125 0.11320098154346701 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2776036.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.64262246328967 #Duplication Level Percentage of deduplicated Percentage of total 1 77.90708121265223 48.803038756210206 2 11.907576604975368 14.918436514363451 3 4.413758550918309 8.294682316478761 4 2.1497767347088788 5.386706094909276 5 1.1176646146500644 3.5006721248051056 6 0.6946420284294417 2.6108519000423547 7 0.4264607160697916 1.8700232342528889 8 0.30102529632069247 1.5085611191453634 9 0.21447038236395002 1.2091488472784069 >10 0.8255946317894305 8.377764938935728 >50 0.030282748122468443 1.2736535035047332 >100 0.010605814466967149 1.117781907724655 >500 5.303322662534972E-4 0.24380819221162714 >1k 4.7140645888813767E-4 0.6053678050331608 >5k 5.892580736101721E-5 0.279502745104335 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7572 0.27276303333242075 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3683 0.1326711901430673 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.8011293801665397E-5 0.0 14-15 0.0 0.0 0.0 3.6022587603330795E-5 0.0 16-17 0.0 0.0 0.0 9.005646900832697E-5 0.0 18-19 0.0 0.0 0.0 1.4409035041332318E-4 0.0 20-21 0.0 0.0 0.0 2.1613552561998477E-4 0.0 22-23 0.0 0.0 0.0 4.1425975743830407E-4 0.0 24-25 0.0 0.0 0.0 6.484065768599543E-4 0.0 26-27 0.0 0.0 0.0 0.0010446550404965929 0.0 28-29 0.0 0.0 0.0 0.0017831180863648742 0.0 30-31 0.0 0.0 0.0 0.0037463491107464024 0.0 32-33 0.0 0.0 0.0 0.007456675633889474 0.0 34-35 0.0 0.0 0.0 0.01271597342397577 0.0 36-37 0.0 0.0 0.0 0.021631563855800142 0.0 38-39 0.0 0.0 0.0 0.03618468924754578 0.0 40-41 0.0 0.0 0.0 0.05518660420830278 0.0 42-43 0.0 0.0 0.0 0.07211722038186824 0.0 44-45 0.0 0.0 0.0 0.09191163226989851 0.0 46-47 5.403388140499619E-5 0.0 0.0 0.11694733065421342 0.0 48-49 7.204517520666159E-5 0.0 0.0 0.1458914797934897 0.0 50-51 7.204517520666159E-5 0.0 0.0 0.1774292552402058 0.0 52-53 7.204517520666159E-5 0.0 0.0 0.20909310974353357 0.0 54-55 7.204517520666159E-5 0.0 0.0 0.24284627432785455 0.0 56-57 7.204517520666159E-5 0.0 0.0 0.28256117716052676 0.0 58-59 7.204517520666159E-5 0.0 0.0 0.3276074229584919 0.0 60-61 7.204517520666159E-5 0.0 0.0 0.38138914625026477 0.0 62-63 7.204517520666159E-5 0.0 0.0 0.43603541164451753 0.0 64-65 7.204517520666159E-5 0.0 0.0 0.4905916205697621 3.6022587603330795E-5 66-67 7.204517520666159E-5 0.0 0.0 0.5555223347247658 3.6022587603330795E-5 68-69 7.204517520666159E-5 0.0 0.0 0.6320162995004388 3.6022587603330795E-5 70-71 9.005646900832697E-5 0.0 0.0 0.7252967900992638 3.6022587603330795E-5 72-73 1.2607905661165777E-4 0.0 0.0 0.8203964213720571 3.6022587603330795E-5 74-75 1.4409035041332318E-4 0.0 0.0 0.9155320752324538 3.6022587603330795E-5 76-77 1.4409035041332318E-4 0.0 0.0 1.0195292856432698 3.6022587603330795E-5 78-79 1.621016442149886E-4 0.0 0.0 1.1391422877801296 3.6022587603330795E-5 80-81 1.8011293801665397E-4 0.0 0.0 1.2839350786517176 3.6022587603330795E-5 82-83 1.8011293801665397E-4 0.0 0.0 1.4375353921923204 3.6022587603330795E-5 84-85 1.9812423181831936E-4 0.0 0.0 1.5932430271077176 3.6022587603330795E-5 86-87 2.1613552561998474E-4 0.0 0.0 1.761108285339239 3.6022587603330795E-5 88-89 2.1613552561998474E-4 0.0 0.0 1.9511094236530073 3.6022587603330795E-5 90-91 2.5215811322331554E-4 0.0 0.0 2.170847208033325 3.6022587603330795E-5 92-93 2.5215811322331554E-4 0.0 0.0 2.4127388837896913 3.6022587603330795E-5 94-95 2.5215811322331554E-4 0.0 0.0 2.651010289491923 3.6022587603330795E-5 96-97 2.5215811322331554E-4 0.0 0.0 2.892757874897876 3.6022587603330795E-5 98-99 3.242032884299771E-4 0.0 0.0 3.167195958553852 3.6022587603330795E-5 100-101 3.4221458223164254E-4 0.0 0.0 3.497937346633833 3.6022587603330795E-5 102-103 3.7823716983497336E-4 0.0 0.0 3.8471222995667205 3.6022587603330795E-5 104-105 4.682936388433003E-4 0.0 0.0 4.1936595923107625 3.6022587603330795E-5 106-107 4.682936388433003E-4 0.0 0.0 4.537603258747366 3.6022587603330795E-5 108-109 4.682936388433003E-4 0.0 0.0 4.909428407988946 3.6022587603330795E-5 110-111 4.863049326449657E-4 0.0 0.0 5.3500206769652845 3.6022587603330795E-5 112-113 5.043162264466311E-4 0.0 0.0 5.821826518099909 3.6022587603330795E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGACCG 90 5.1595507E-6 39.665066 9 GTATCAA 14100 0.0 33.462208 1 GTACATG 16145 0.0 32.692204 1 TACATGG 16140 0.0 31.55812 2 CGCATAG 390 0.0 28.986532 8 TATCAAC 16565 0.0 28.374886 2 ATCAACG 16050 0.0 28.208961 3 ACATGGG 17065 0.0 28.032238 3 TCAACGC 16130 0.0 27.773588 4 CAACGCA 16210 0.0 27.490013 5 AACGCAG 16195 0.0 27.013342 6 CATGGGA 13090 0.0 24.393475 4 ACGCAGA 18100 0.0 23.77089 7 CGCAGAT 1155 0.0 23.696442 8 GAGTACT 5295 0.0 23.369942 12-13 ATGGGAG 5245 0.0 23.170801 5 CGCAGAG 17485 0.0 22.696949 8 GGTATCA 9875 0.0 22.20032 1 GTATAGG 945 0.0 22.064026 1 ACGCATA 540 0.0 22.040524 7 >>END_MODULE