FastQCFastQC Report
Thu 26 May 2016
SRR1364617_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364617_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2776036
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129830.46768125485404366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110040.3963925539870521No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71670.2581738853530718No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31190.11235445073478874No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122300.037.7062761
GTATCAA184250.037.3162041
ATCAACG219450.031.2357733
TATCAAC219550.031.1909482
TCAACGC222800.030.7416274
CAACGCA224050.030.1251565
AACGCAG227800.029.6975146
GTACATG156450.029.0568241
TACATGG153450.028.8715572
GAGTACT139550.026.58172812-13
ACATGGG162500.026.2177263
CGCAGAG258150.025.9527688
ACGCAGA259750.025.8616877
ATAACGC3550.025.1419123
TAACGCA3800.025.05554
AGTACTT146950.024.87847712-13
GTACTTT151050.024.53825214-15
GCAGAGT271100.023.8128249
CATGGGA125150.022.7756794
ACTTTTT185000.020.73049216-17
Produced by FastQC (version 0.11.5)