##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364617_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2776036 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.44559688707207 31.0 30.0 33.0 26.0 34.0 2 29.480660913619275 31.0 30.0 34.0 25.0 34.0 3 29.491966602738582 31.0 30.0 34.0 25.0 34.0 4 33.127772478454894 35.0 33.0 37.0 27.0 37.0 5 32.53298048008023 35.0 33.0 37.0 25.0 37.0 6 32.53272976287051 35.0 33.0 37.0 25.0 37.0 7 32.314969978775494 35.0 32.0 37.0 23.0 37.0 8 32.23090514676323 35.0 32.0 37.0 22.0 37.0 9 33.44746249688404 37.0 33.0 39.0 19.0 39.0 10-11 33.243945143362694 37.0 33.0 39.0 18.0 39.0 12-13 33.09722370315082 37.0 32.0 39.0 17.0 39.0 14-15 34.01641585339671 38.0 33.0 41.0 17.0 41.0 16-17 33.94099247992461 38.0 33.0 41.0 17.0 41.0 18-19 34.01724167121752 38.5 33.0 41.0 17.5 41.0 20-21 34.056401826201096 39.0 33.0 41.0 18.0 41.0 22-23 34.048265584452075 39.0 33.0 41.0 18.0 41.0 24-25 34.049189924049976 39.0 33.0 41.0 18.0 41.0 26-27 33.94625087715001 38.5 33.0 41.0 17.5 41.0 28-29 33.70663781017249 38.0 32.0 41.0 16.0 41.0 30-31 33.615621699430406 38.0 32.0 40.0 16.0 41.0 32-33 33.41085544279685 38.0 32.0 40.0 15.5 41.0 34-35 33.268153583022695 38.0 32.0 40.0 13.5 41.0 36-37 33.08781442315589 38.0 32.0 40.0 9.0 41.0 38-39 32.947444305477305 38.0 32.0 40.0 9.0 41.0 40-41 32.77075117181478 38.0 31.0 40.0 8.0 41.0 42-43 32.57633078245383 38.0 31.0 40.0 4.5 41.0 44-45 32.34109049738548 38.0 31.0 40.0 2.0 41.0 46-47 32.039211307057975 38.0 30.0 40.0 2.0 41.0 48-49 31.746389095818643 38.0 30.0 40.0 2.0 41.0 50-51 31.539294879461217 37.5 30.0 40.0 2.0 41.0 52-53 31.373165189500426 37.0 30.0 40.0 2.0 41.0 54-55 31.168763301340473 37.0 29.0 40.0 2.0 41.0 56-57 30.956257951986213 37.0 29.0 40.0 2.0 41.0 58-59 30.777098171637544 36.5 28.5 40.0 2.0 41.0 60-61 30.572479787726095 36.0 28.0 40.0 2.0 41.0 62-63 30.31559316954103 36.0 28.0 40.0 2.0 41.0 64-65 30.060657894926436 35.5 28.0 39.0 2.0 41.0 66-67 29.797089627079764 35.0 28.0 39.0 2.0 41.0 68-69 29.489463393126027 35.0 28.0 39.0 2.0 40.0 70-71 29.24158422297117 35.0 28.0 38.0 2.0 40.0 72-73 29.087571991141324 35.0 28.0 37.5 2.0 40.0 74-75 28.801018970935537 34.5 27.5 37.0 2.0 39.5 76-77 28.495215479914528 34.0 26.5 36.5 2.0 39.0 78-79 28.177629720940217 34.0 26.0 36.0 2.0 39.0 80-81 27.89731004929331 34.0 26.0 36.0 2.0 37.5 82-83 27.6313931087349 34.0 26.0 35.0 2.0 37.0 84-85 27.590537910891648 34.0 26.0 35.0 2.0 37.0 86-87 27.42006029460713 34.0 26.0 35.0 2.0 36.0 88-89 27.258412354883006 34.0 26.0 35.0 2.0 36.0 90-91 27.090629588377098 34.0 26.0 35.0 2.0 36.0 92-93 26.94941456090627 34.0 26.0 35.0 2.0 35.5 94-95 26.858934826493602 34.0 25.5 35.0 2.0 35.0 96-97 26.763442368902997 34.0 25.0 35.0 2.0 35.0 98-99 26.670158996497165 34.0 25.0 35.0 2.0 35.0 100-101 26.56497826397064 34.0 25.0 35.0 2.0 35.0 102-103 25.408793329769498 31.5 22.0 34.0 2.0 35.0 104-105 26.312032877095255 33.5 24.5 35.0 2.0 35.0 106-107 26.481365335319857 34.0 25.0 35.0 2.0 35.0 108-109 26.490045338028757 34.0 25.0 35.0 2.0 35.0 110-111 26.426927820820765 34.0 25.0 35.0 2.0 35.0 112-113 26.372150973546454 34.0 25.0 35.0 2.0 35.0 114-115 26.283674455230408 34.0 24.0 35.0 2.0 35.0 116-117 26.166063768625477 34.0 24.0 35.0 2.0 35.0 118-119 26.066709689643794 34.0 24.0 35.0 2.0 35.0 120-121 25.93190037881353 34.0 23.0 35.0 2.0 35.0 122-123 25.74737809596129 34.0 21.5 35.0 2.0 35.0 124-125 24.963670139724414 33.0 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.760356460033293 1101 2 0.729295818929014 1101 3 0.6013282230721657 1101 4 0.5436043278948262 1101 5 0.9568988535555789 1101 6 1.0003699122475425 1101 7 0.9169687296089677 1101 8 0.9943345721772303 1101 9 1.1291730212760243 1101 10-11 1.138086564509372 1101 12-13 1.122788508908819 1101 14-15 1.2191463267825355 1101 16-17 1.3073262363520328 1101 18-19 1.3579081775775492 1101 20-21 1.3228074443968225 1101 22-23 1.21886304405583 1101 24-25 1.1515209913607052 1101 26-27 1.3391052586785577 1101 28-29 1.4297864174394306 1101 30-31 1.3319077253717708 1101 32-33 1.42583476301931 1101 34-35 1.3297633483137972 1101 36-37 1.337270739472153 1101 38-39 1.3568923419425403 1101 40-41 1.4109648887344406 1101 42-43 1.3694319180576713 1101 44-45 1.3154439134593119 1101 46-47 1.355897496670849 1101 48-49 1.3816007442129745 1101 50-51 1.3107918040142863 1101 52-53 1.3085378245830412 1101 54-55 1.2919451295911593 1101 56-57 1.3616739537101168 1101 58-59 1.4245769607497962 1101 60-61 1.3994038930563661 1101 62-63 1.3908603512129147 1101 64-65 1.4030096830346075 1101 66-67 1.2407221926845402 1101 68-69 1.2445711672293953 1101 70-71 1.2942651635137281 1101 72-73 1.2640569494163003 1101 74-75 1.2359875669037947 1101 76-77 1.2162502024277622 1101 78-79 1.2404597992278141 1101 80-81 1.1862041113198885 1101 82-83 1.1269377803804126 1101 84-85 1.2319329898676905 1101 86-87 1.16279256030872 1101 88-89 1.1609587732820863 1101 90-91 1.1571995754734914 1101 92-93 1.16179151314412 1101 94-95 1.062336925383498 1101 96-97 1.149363700958883 1101 98-99 1.1976188195586488 1101 100-101 1.2127419597535685 1101 102-103 1.2016488977163426 1101 104-105 1.0878960257635413 1101 106-107 1.1434585514348825 1101 108-109 1.146307692042587 1101 110-111 1.1069458888248143 1101 112-113 1.1681412695134057 1101 114-115 1.1154376898291432 1101 116-117 1.1372927063194282 1101 118-119 1.1440243415670999 1101 120-121 1.0317450927445826 1101 122-123 1.0501461184054968 1101 124-125 0.981498642589905 1102 1 0.42524080055742175 1102 2 -0.573953951061366 1102 3 0.04407996816422255 1102 4 0.3328057999563967 1102 5 0.8502531829387152 1102 6 0.7732376032315145 1102 7 0.6353495670894844 1102 8 0.5342034520800638 1102 9 0.8706325039268137 1102 10-11 0.893586074094955 1102 12-13 0.9469030741584774 1102 14-15 1.0041625005213461 1102 16-17 0.9794042958307116 1102 18-19 1.0957090167181178 1102 20-21 1.01282353630247 1102 22-23 1.0495050894638709 1102 24-25 0.9798251219477194 1102 26-27 0.9703503267695908 1102 28-29 1.0712512484714978 1102 30-31 0.9940662034621184 1102 32-33 0.9729076494734628 1102 34-35 0.7850410315937708 1102 36-37 0.8388396564337128 1102 38-39 0.7217872038724664 1102 40-41 0.6437626240603009 1102 42-43 0.5919722471906255 1102 44-45 0.4008781669480399 1102 46-47 0.45574771276883297 1102 48-49 0.5628874595352968 1102 50-51 0.47866764324253097 1102 52-53 0.5785599721422443 1102 54-55 0.6128753036970416 1102 56-57 0.40920934362756967 1102 58-59 0.5831639184151598 1102 60-61 0.5328971517578651 1102 62-63 0.6622461021899433 1102 64-65 0.6463108793184489 1102 66-67 0.5575463589508125 1102 68-69 0.42778856051603853 1102 70-71 0.38795290455621156 1102 72-73 0.35738275409925535 1102 74-75 0.39540255920563183 1102 76-77 0.454100609080097 1102 78-79 0.5043549230238469 1102 80-81 0.45477813350134966 1102 82-83 0.31904225443286904 1102 84-85 0.3971209898536614 1102 86-87 0.40114510105286527 1102 88-89 0.45778627717208664 1102 90-91 0.42156553217907344 1102 92-93 0.5151550604477748 1102 94-95 0.35076200537540814 1102 96-97 0.3075090226157968 1102 98-99 0.31534417345782373 1102 100-101 0.1493294762224906 1102 102-103 0.15585821991569304 1102 104-105 0.24567511482022297 1102 106-107 0.5124169848676985 1102 108-109 0.5045994073707547 1102 110-111 0.47282695719173873 1102 112-113 0.5172537304819116 1102 114-115 0.5258975454053143 1102 116-117 0.532617841139551 1102 118-119 0.43596006396097664 1102 120-121 0.5001862529516892 1102 122-123 0.47234286518682467 1102 124-125 0.2777744741024506 1103 1 0.29076958461444136 1103 2 -1.0037929094671618 1103 3 -0.1259501773356675 1103 4 0.24687610103799784 1103 5 0.4470415831691028 1103 6 0.5481454217446071 1103 7 0.574841583221577 1103 8 0.584454909592786 1103 9 0.6447938736433017 1103 10-11 0.8042845952807482 1103 12-13 0.8274362493502068 1103 14-15 0.8782578635595399 1103 16-17 0.9988422776316881 1103 18-19 1.0420428235158283 1103 20-21 1.0331717340261406 1103 22-23 1.025781642451065 1103 24-25 0.8554291124556883 1103 26-27 0.9786818396514789 1103 28-29 1.134349957710164 1103 30-31 0.9607833135397925 1103 32-33 1.1052559201132013 1103 34-35 1.152484391542444 1103 36-37 1.213865581328406 1103 38-39 1.1870999446902673 1103 40-41 1.2638574972672032 1103 42-43 1.2749202377503508 1103 44-45 1.2331982708181428 1103 46-47 1.1152423103647777 1103 48-49 1.1829468743662517 1103 50-51 1.1303389940802262 1103 52-53 1.179897658486155 1103 54-55 1.1536160074134933 1103 56-57 1.115554187800356 1103 58-59 1.0157434623428259 1103 60-61 1.0118088265898564 1103 62-63 1.039301122357994 1103 64-65 0.9960231107822608 1103 66-67 1.006875634073996 1103 68-69 1.0022948408530183 1103 70-71 1.086120506243983 1103 72-73 1.0049327596708686 1103 74-75 0.9559903097186151 1103 76-77 0.9512584997007103 1103 78-79 0.9148317852574692 1103 80-81 0.9680784644755924 1103 82-83 0.966496792884481 1103 84-85 0.9766590077892516 1103 86-87 0.9508532456889327 1103 88-89 0.9701201762909477 1103 90-91 1.0093550023350844 1103 92-93 0.9769820900208259 1103 94-95 0.9690962202678683 1103 96-97 0.9501202291678901 1103 98-99 0.9712935214911909 1103 100-101 1.1211631182240254 1103 102-103 1.1418960286586213 1103 104-105 1.1115106461710482 1103 106-107 1.1253059869202318 1103 108-109 1.0699870341616453 1103 110-111 1.1163983330502276 1103 112-113 1.091205084295055 1103 114-115 1.042648305658183 1103 116-117 1.121581201111752 1103 118-119 1.1575541561133562 1103 120-121 1.1151615364643384 1103 122-123 1.2013972996742375 1103 124-125 1.091233662129163 1104 1 0.3420326065214816 1104 2 -0.645449536026323 1104 3 0.17463103633563293 1104 4 0.29127764774664655 1104 5 0.5443300968479221 1104 6 0.46242296394905225 1104 7 0.6413097411564976 1104 8 0.7236675090754972 1104 9 0.6609950816658525 1104 10-11 0.7861451357546514 1104 12-13 0.7259981565364129 1104 14-15 0.8622585389513304 1104 16-17 1.0739807520122397 1104 18-19 0.8341532993557479 1104 20-21 0.8681698159202966 1104 22-23 0.7988187879180444 1104 24-25 0.7603606618851444 1104 26-27 0.6326433670180194 1104 28-29 0.8691587500728915 1104 30-31 0.8525919288597308 1104 32-33 0.8654727716390553 1104 34-35 0.669506592116619 1104 36-37 0.7586474485044263 1104 38-39 0.7925086389538691 1104 40-41 0.8274014329882107 1104 42-43 0.7618168517600168 1104 44-45 0.9306803421766929 1104 46-47 0.9313390398079378 1104 48-49 0.8798368966476886 1104 50-51 0.9600685306281775 1104 52-53 0.8484047506974548 1104 54-55 1.0459088014759637 1104 56-57 0.9984300524631777 1104 58-59 0.8063693054799543 1104 60-61 0.9164417763646213 1104 62-63 0.8701245568595333 1104 64-65 0.7914760020059965 1104 66-67 0.7962455888026021 1104 68-69 0.8497327824036276 1104 70-71 0.9059373252863878 1104 72-73 0.8364589590162694 1104 74-75 0.8113322074614082 1104 76-77 0.7730215374568026 1104 78-79 0.7348777185003286 1104 80-81 0.7490127655517966 1104 82-83 0.8297306960042299 1104 84-85 0.7939399492039065 1104 86-87 0.8071376074838135 1104 88-89 0.7464287151541136 1104 90-91 0.7002532557689705 1104 92-93 0.7554179878113061 1104 94-95 0.7011743082603559 1104 96-97 0.7460030651965042 1104 98-99 0.854584876163333 1104 100-101 0.8626496562282142 1104 102-103 0.8686831492991978 1104 104-105 0.8387213947570231 1104 106-107 0.8824567145285407 1104 108-109 0.7710066321897173 1104 110-111 0.7493427868561433 1104 112-113 0.7485906055864859 1104 114-115 0.7588893155862912 1104 116-117 0.7270006511090585 1104 118-119 0.6075759891146006 1104 120-121 0.6295070674256849 1104 122-123 0.7255951965242495 1104 124-125 0.6842298697940414 1105 1 0.6151456696698077 1105 2 0.330417343982365 1105 3 0.612941174343618 1105 4 0.609171003219835 1105 5 0.691304745179103 1105 6 0.672636129039077 1105 7 0.7358909628522596 1105 8 0.6742597245843172 1105 9 0.8713799485813567 1105 10-11 0.9927904804597034 1105 12-13 0.9879468175793207 1105 14-15 0.9729350263392575 1105 16-17 1.1465729287963526 1105 18-19 1.02661482696341 1105 20-21 1.0174550945529646 1105 22-23 0.9050617405484829 1105 24-25 0.9350967023208696 1105 26-27 1.0350516047441474 1105 28-29 1.1405721265019295 1105 30-31 1.0110468973018598 1105 32-33 0.9295912741632293 1105 34-35 0.9451072730929582 1105 36-37 0.9678390544995921 1105 38-39 0.9312624920908306 1105 40-41 0.8615855082503572 1105 42-43 0.8684140576382902 1105 44-45 0.9024772880207266 1105 46-47 0.9600950339320207 1105 48-49 0.9163327706294524 1105 50-51 1.019253554534572 1105 52-53 0.8776268880146176 1105 54-55 0.772457008349182 1105 56-57 0.7463781433180365 1105 58-59 0.681065169023853 1105 60-61 0.786149579472589 1105 62-63 0.7560353148635599 1105 64-65 0.8598904441436481 1105 66-67 0.7957736313383599 1105 68-69 0.806729403672243 1105 70-71 0.7601922144763975 1105 72-73 0.6708571385540658 1105 74-75 0.6686460987622453 1105 76-77 0.7835938575869044 1105 78-79 0.7443466902667879 1105 80-81 0.5407742827327198 1105 82-83 0.5344681530078326 1105 84-85 0.513632295809245 1105 86-87 0.5123814346473985 1105 88-89 0.5695287643500855 1105 90-91 0.5982130753162487 1105 92-93 0.4755098221376315 1105 94-95 0.5264635000922446 1105 96-97 0.624601278362487 1105 98-99 0.6857677637562816 1105 100-101 0.7366451087448738 1105 102-103 0.31872342634115114 1105 104-105 0.2113667957686971 1105 106-107 0.5061860180879094 1105 108-109 0.5895965874214966 1105 110-111 0.5827135095694906 1105 112-113 0.3973096465399877 1105 114-115 0.47259179449105915 1105 116-117 0.17685108802510285 1105 118-119 0.15160796526907916 1105 120-121 0.1932680061839953 1105 122-123 0.31124422330428203 1105 124-125 0.15810379013396414 1106 1 0.5806370702364276 1106 2 0.566526960845529 1106 3 0.7330533435280557 1106 4 0.48046836614190624 1106 5 0.618014944755231 1106 6 0.8290844357864628 1106 7 0.7049736972472829 1106 8 0.8845816468198677 1106 9 0.8647950165484986 1106 10-11 0.9994245586719899 1106 12-13 0.890549750124741 1106 14-15 1.0315142655061038 1106 16-17 1.1748087482375453 1106 18-19 1.2126737888211636 1106 20-21 1.2190446317851027 1106 22-23 1.0838273875111852 1106 24-25 1.1501950427872316 1106 26-27 1.1917245406920784 1106 28-29 1.2278070323380703 1106 30-31 1.1258511151046093 1106 32-33 1.110523125085976 1106 34-35 1.0764097922637959 1106 36-37 1.2322516772939736 1106 38-39 1.3012065037136011 1106 40-41 1.31110522051506 1106 42-43 1.3295374070045511 1106 44-45 1.1823151417092177 1106 46-47 1.0341985616983607 1106 48-49 1.0194133559314764 1106 50-51 1.1738672710309785 1106 52-53 1.2511275195348261 1106 54-55 1.1429863460489322 1106 56-57 0.9467617677891838 1106 58-59 1.047590705794157 1106 60-61 1.123470860129089 1106 62-63 1.1322703654595223 1106 64-65 1.0998373444028182 1106 66-67 0.998374409980034 1106 68-69 0.9851848119605506 1106 70-71 1.0583216857400402 1106 72-73 1.2081470072031095 1106 74-75 1.190693216992102 1106 76-77 1.210457181132547 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-2.1385572585717654 2216 112-113 -2.051896425094963 2216 114-115 -2.245156193002007 2216 116-117 -2.261015007150995 2216 118-119 -2.3959034732789384 2216 120-121 -2.4407152398628362 2216 122-123 -2.398097366350875 2216 124-125 -2.5213915442147474 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 121375.0 3 37898.0 4 17196.0 5 13240.0 6 14765.0 7 17441.0 8 19624.0 9 20631.0 10 22462.0 11 24700.0 12 27936.0 13 31754.0 14 36360.0 15 29555.0 16 19210.0 17 15854.0 18 14134.0 19 13175.0 20 12794.0 21 13211.0 22 15418.0 23 20485.0 24 28635.0 25 38087.0 26 43588.0 27 48234.0 28 55871.0 29 64450.0 30 74169.0 31 88817.0 32 106706.0 33 131595.0 34 167763.0 35 227724.0 36 348290.0 37 555453.0 38 236261.0 39 1175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42234632361051 19.894796560307434 13.82155783846363 23.861299277618425 2 15.192048692422164 21.30315099149815 39.648498333083396 23.856301982996296 3 19.194419668909195 26.264212711938896 29.5012024339742 25.040165185177717 4 12.429822842304578 18.052425362918218 37.131137619279556 32.386614175497655 5 12.452553618824764 39.46357033162561 34.05423246582816 14.029643583721468 6 30.33124257933626 38.35204349229404 17.367703254141162 13.949010674228541 7 26.967032824525923 34.26424172038239 20.740524071391057 18.028201383700633 8 25.572579029954944 36.95733772904962 19.048131940652066 18.421951300343366 9 26.179820289220235 16.623067014022975 19.931873591506676 37.26523910525012 10-11 24.136548312636876 28.38802647646976 26.842694677168446 20.632730533724917 12-13 25.493545811221484 25.53641702232059 27.91668790713015 21.053349259327774 14-15 22.416486615252566 26.485075171277757 26.711259975628888 24.387178237840786 16-17 20.959483193293966 29.785242939734598 27.341843459381376 21.913430407590056 18-19 20.86789032036932 27.893623647146526 30.517388047429595 20.72109798505456 20-21 22.02960229215295 26.750376747602704 30.6917889377609 20.528232022483447 22-23 21.337872312216803 26.90126784214643 30.547246223512165 21.213613622124605 24-25 20.97996927993729 26.837890430815737 30.98106436660043 21.201075922646538 26-27 21.173698756068006 26.99269750104105 30.78985647160195 21.04374727128899 28-29 20.90429464812012 26.917025471558198 30.962665129390516 21.21601475093117 30-31 21.209090948388276 26.853614290304595 30.987890646951264 20.949404114355865 32-33 20.918953287726996 26.908855770858775 30.79618362134492 21.37600732006931 34-35 21.181371567227515 26.797401042349595 30.891440168643346 21.129787221779544 36-37 21.300975402845605 26.762510885589414 30.76781341717001 21.168700294394966 38-39 21.27920891515816 26.803506870948357 30.84063030882885 21.07665390506463 40-41 21.39437312772601 26.838304690573178 30.672638971540717 21.0946832101601 42-43 21.24808938524946 26.92234951848726 30.451965983402374 21.37759511286091 44-45 21.37881136988137 27.019012001285287 30.11581982366223 21.486356805171113 46-47 21.42387562697314 27.22432273933047 29.504138274863873 21.847663358832524 48-49 21.57929403899055 27.28002185854159 29.29222885259047 21.84845524987739 50-51 21.746756288802104 27.584238689450082 28.9822085568018 21.686796464946017 52-53 21.71613079155508 27.70958800779025 28.84723556308988 21.727045637564792 54-55 21.64103779633982 27.622948693748928 28.86120713131962 21.874806378591632 56-57 21.693427317567828 27.87425060721416 28.587669837598916 21.844652237619087 58-59 21.716833232139862 27.575565946472945 28.81252779368275 21.895073027704438 60-61 21.766918728719656 27.52257175339225 28.90702786275106 21.803481655137034 62-63 21.828661443871766 27.451805380045503 28.905695027009738 21.813838149072996 64-65 21.705113181780685 27.4538629210658 29.007273359963776 21.83375053718974 66-67 21.684066777231994 27.601407186362138 28.846978209216307 21.86754782718956 68-69 21.628994614229946 27.588157717940692 28.872586967164132 21.910260700665233 70-71 21.509159103124023 27.383380474892977 28.89809426102543 22.209366160957565 72-73 21.641210099532778 27.482915881655785 28.730263655587056 22.145610363224385 74-75 21.607340183234403 27.62104178655795 28.630461075973024 22.141156954234628 76-77 21.67213521633717 27.410591149108683 28.8600915229345 22.057182111619653 78-79 21.589399202274482 27.504635687635254 28.77215291330116 22.133812196789105 80-81 21.41900987716494 27.682935044284957 28.814473636337766 22.083581442212335 82-83 21.51929832208316 27.70597163612877 28.687930954673966 22.086799087114105 84-85 21.61037623992065 27.46386977539364 28.66475542721101 22.2609985574747 86-87 21.59368017788008 27.40271845289717 28.82584916756048 22.17775220166227 88-89 21.70530209262416 27.268450409144553 28.8285526556572 22.197694842574087 90-91 21.586409542239366 27.479596806381473 28.841736922720024 22.092256728659137 92-93 21.457282042183436 27.523886805945203 28.81469746681425 22.204133685057112 94-95 21.38624768632096 27.58950128283551 28.692033739186318 22.332217291657212 96-97 21.40840280674755 27.534996084127496 28.5481515424647 22.508449566660254 98-99 21.375821096067565 27.409398003973322 28.553141823801386 22.66163907615773 100-101 21.44685166123174 27.551204013649 28.51998043967504 22.481963885444216 102-103 21.361942482713655 27.387172712159607 28.68540922574823 22.565475579378504 104-105 21.224732986062268 27.286835081305988 28.805715221275886 22.68271671135586 106-107 21.136017438451805 27.454087983704227 28.68954580880781 22.720348769036157 108-109 21.15327253365765 27.398832519420786 28.50919632319989 22.938698623721674 110-111 21.080261028049655 27.415906241427713 28.556584625454363 22.947248105068276 112-113 21.192976351102473 27.330518816072757 28.462467729684583 23.01403710314019 114-115 21.020414044516812 27.292410384613998 28.506896539302527 23.180279031566663 116-117 20.900444671150844 27.272113239845762 28.6160150778967 23.211427011106693 118-119 20.765479089059134 27.326388776316318 28.578628069944134 23.329504064680417 120-121 20.770013234287234 27.384292754610517 28.50620797411289 23.339486036989353 122-123 20.70462515512344 27.294449057371807 28.301594191674035 23.699331595830714 124-125 20.622054098411148 27.274674157550272 28.403512036128287 23.699759707910296 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 953.0 1 703.0 2 675.0 3 970.5 4 759.5 5 640.0 6 1448.5 7 3174.0 8 5047.0 9 5760.0 10 4576.0 11 3090.5 12 2733.5 13 2861.0 14 3084.0 15 3373.0 16 3811.5 17 4193.5 18 4589.0 19 5091.0 20 5670.0 21 6636.5 22 7905.0 23 9423.0 24 11402.5 25 14119.5 26 17915.0 27 22875.5 28 27879.5 29 32871.5 30 38429.0 31 43553.5 32 48709.5 33 55014.0 34 61488.0 35 67165.5 36 73060.5 37 79210.5 38 84350.5 39 87534.0 40 90589.5 41 94592.5 42 97630.0 43 100340.5 44 104234.0 45 107509.5 46 109097.5 47 109515.5 48 114493.0 49 114008.0 50 106453.5 51 99539.5 52 91503.5 53 84650.0 54 77668.0 55 69847.5 56 60991.0 57 53481.5 58 47679.0 59 42102.5 60 35256.0 61 27898.5 62 21722.0 63 16375.0 64 11718.0 65 8567.5 66 6324.5 67 4714.0 68 3569.0 69 2692.5 70 2076.5 71 1549.5 72 1235.0 73 1140.0 74 890.5 75 639.5 76 469.5 77 309.0 78 211.0 79 143.0 80 99.0 81 70.0 82 45.5 83 34.0 84 25.5 85 21.0 86 15.0 87 10.0 88 8.0 89 5.5 90 2.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05770818534053593 2 0.07391834976203479 3 0.0 4 0.007348607871079482 5 0.028746024907457972 6 1.4409035041332318E-4 7 7.204517520666159E-5 8 0.0 9 7.204517520666159E-4 10-11 0.05936522437028915 12-13 0.010104335822734288 14-15 0.007510709515294469 16-17 0.05255695531325963 18-19 1.9812423181831936E-4 20-21 0.010986889219015892 22-23 0.013508470351249047 24-25 0.0 26-27 0.0 28-29 0.05156633415416803 30-31 0.0 32-33 0.0038183942859530643 34-35 0.0 36-37 1.2607905661165777E-4 38-39 0.0 40-41 0.0 42-43 0.003494190997523087 44-45 0.0 46-47 0.0 48-49 1.6210164421498856E-4 50-51 3.782371698349733E-4 52-53 1.8011293801665397E-5 54-55 0.0 56-57 0.0013868696227282355 58-59 1.8011293801665397E-5 60-61 0.0 62-63 0.0 64-65 5.403388140499619E-4 66-67 0.0 68-69 6.123839892566234E-4 70-71 0.0 72-73 0.0023774907818198323 74-75 0.003007886064878121 76-77 3.782371698349733E-4 78-79 6.664178706616196E-4 80-81 0.0013328357413232392 82-83 1.6210164421498856E-4 84-85 5.403388140499619E-5 86-87 3.061919946283117E-4 88-89 0.0 90-91 0.0 92-93 0.006610144825211201 94-95 0.005547478490912942 96-97 0.005547478490912942 98-99 7.564743396699466E-4 100-101 3.061919946283117E-4 102-103 3.6022587603330795E-5 104-105 3.782371698349733E-4 106-107 0.0036562926417380757 108-109 0.005187252614879634 110-111 4.5028234504163495E-4 112-113 0.0025756150136381517 114-115 2.1613552561998477E-4 116-117 0.0018731745553732012 118-119 0.0017651067925632089 120-121 0.010500584286370926 122-123 7.564743396699466E-4 124-125 0.0014228922103315664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2776036.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.69997373621706 #Duplication Level Percentage of deduplicated Percentage of total 1 75.78132525142284 43.7258047670282 2 12.454125225530149 14.37205396841296 3 4.867400092051386 8.425465724250765 4 2.4178694507430705 5.58044015221907 5 1.3494466716442584 3.8931518756149615 6 0.8084987410392607 2.7990213674237943 7 0.5366901262480148 2.167690433229823 8 0.3815854261148062 1.7613975251958014 9 0.2678297727425815 1.3908393767721545 >10 1.07062644705679 10.149872201311837 >50 0.04483200767454056 1.7588932514280575 >100 0.01782787998796662 1.751951182693257 >500 0.001190814424000999 0.5191579999769241 >1k 5.640699902963107E-4 0.5776337930776415 >5k 6.267444336625674E-5 0.25918120547094825 >10k+ 1.253488867325135E-4 0.8674451758939076 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12983 0.46768125485404366 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11004 0.3963925539870521 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7167 0.2581738853530718 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3119 0.11235445073478874 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.6022587603330795E-5 0.0 2 0.0 0.0 0.0 3.6022587603330795E-5 0.0 3 0.0 0.0 0.0 3.6022587603330795E-5 0.0 4 0.0 0.0 0.0 3.6022587603330795E-5 0.0 5 0.0 0.0 0.0 3.6022587603330795E-5 0.0 6 0.0 0.0 0.0 3.6022587603330795E-5 0.0 7 0.0 0.0 0.0 3.6022587603330795E-5 0.0 8 0.0 0.0 0.0 3.6022587603330795E-5 0.0 9 0.0 0.0 0.0 3.6022587603330795E-5 0.0 10-11 3.6022587603330795E-5 0.0 0.0 3.6022587603330795E-5 0.0 12-13 3.6022587603330795E-5 0.0 0.0 3.6022587603330795E-5 0.0 14-15 3.6022587603330795E-5 0.0 0.0 3.6022587603330795E-5 0.0 16-17 3.6022587603330795E-5 0.0 0.0 9.005646900832697E-5 0.0 18-19 5.403388140499619E-5 0.0 0.0 1.4409035041332318E-4 0.0 20-21 7.204517520666159E-5 0.0 0.0 1.621016442149886E-4 0.0 22-23 7.204517520666159E-5 0.0 0.0 3.4221458223164254E-4 0.0 24-25 7.204517520666159E-5 0.0 0.0 6.484065768599543E-4 0.0 26-27 7.204517520666159E-5 0.0 0.0 0.0010086324528932621 0.0 28-29 7.204517520666159E-5 0.0 0.0 0.0017651067925632089 0.0 30-31 7.204517520666159E-5 0.0 0.0 0.0038904394611597257 0.0 32-33 7.204517520666159E-5 0.0 0.0 0.0077808789223194515 0.0 34-35 7.204517520666159E-5 0.0 0.0 0.013292334825629061 0.0 36-37 7.204517520666159E-5 0.0 0.0 0.022604173721090075 0.0 38-39 7.204517520666159E-5 0.0 0.0 0.037931784746307325 0.0 40-41 7.204517520666159E-5 0.0 0.0 0.057654151459130935 0.0 42-43 7.204517520666159E-5 0.0 0.0 0.07496300480253137 0.0 44-45 7.204517520666159E-5 0.0 0.0 0.09477542798436331 0.0 46-47 7.204517520666159E-5 0.0 0.0 0.12000925060049653 0.0 48-49 7.204517520666159E-5 0.0 0.0 0.14909749009018614 0.0 50-51 7.204517520666159E-5 0.0 0.0 0.18079736718111725 0.0 52-53 7.204517520666159E-5 0.0 0.0 0.21249724427204836 0.0 54-55 7.204517520666159E-5 0.0 0.0 0.24601626203694765 0.0 56-57 1.4409035041332318E-4 0.0 0.0 0.2860733794518515 0.0 58-59 1.4409035041332318E-4 0.0 0.0 0.33131774948163495 0.0 60-61 1.4409035041332318E-4 0.0 0.0 0.38468521301596953 0.0 62-63 1.4409035041332318E-4 0.0 0.0 0.43846693630774236 0.0 64-65 1.4409035041332318E-4 0.0 0.0 0.49273496453216026 0.0 66-67 1.4409035041332318E-4 0.0 0.0 0.5573774979863373 0.0 68-69 1.8011293801665397E-4 0.0 0.0 0.6327727738401088 0.0 70-71 1.8011293801665397E-4 0.0 0.0 0.7246123609348005 0.0 72-73 1.8011293801665397E-4 0.0 0.0 0.8180549531778406 0.0 74-75 1.8011293801665397E-4 0.0 0.0 0.9123620875233607 0.0 76-77 1.8011293801665397E-4 0.0 0.0 1.0154407219502917 0.0 78-79 1.8011293801665397E-4 0.0 0.0 1.1349276450305399 0.0 80-81 2.5215811322331554E-4 0.0 0.0 1.2786937921554333 0.0 82-83 2.5215811322331554E-4 0.0 0.0 1.4318078007633908 0.0 84-85 2.5215811322331554E-4 0.0 0.0 1.5856782837110182 0.0 86-87 2.5215811322331554E-4 0.0 0.0 1.7520305932631999 0.0 88-89 2.5215811322331554E-4 0.0 0.0 1.941329291118703 0.0 90-91 2.5215811322331554E-4 0.0 0.0 2.159283957412656 0.0 92-93 2.5215811322331554E-4 0.0 0.0 2.3987801310934005 0.0 94-95 2.5215811322331554E-4 0.0 0.0 2.6357006897605073 0.0 96-97 2.5215811322331554E-4 0.0 0.0 2.8759713490747236 0.0 98-99 2.8818070082664636E-4 0.0 0.0 3.150121252029873 3.6022587603330795E-5 100-101 3.061919946283117E-4 0.0 0.0 3.4795117930747295 3.6022587603330795E-5 102-103 3.4221458223164254E-4 0.0 0.0 3.8274179441476983 3.6022587603330795E-5 104-105 4.682936388433003E-4 0.0 0.0 4.172532344681409 3.6022587603330795E-5 106-107 5.223275202482964E-4 0.0 0.0 4.515107152789085 3.6022587603330795E-5 108-109 5.403388140499619E-4 0.0 0.0 4.883672257852563 3.6022587603330795E-5 110-111 5.403388140499619E-4 0.0 0.0 5.3231298153193976 3.6022587603330795E-5 112-113 5.403388140499619E-4 0.0 0.0 5.795079746804436 3.6022587603330795E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12230 0.0 37.706276 1 GTATCAA 18425 0.0 37.316204 1 ATCAACG 21945 0.0 31.235773 3 TATCAAC 21955 0.0 31.190948 2 TCAACGC 22280 0.0 30.741627 4 CAACGCA 22405 0.0 30.125156 5 AACGCAG 22780 0.0 29.697514 6 GTACATG 15645 0.0 29.056824 1 TACATGG 15345 0.0 28.871557 2 GAGTACT 13955 0.0 26.581728 12-13 ACATGGG 16250 0.0 26.217726 3 CGCAGAG 25815 0.0 25.952768 8 ACGCAGA 25975 0.0 25.861687 7 ATAACGC 355 0.0 25.141912 3 TAACGCA 380 0.0 25.0555 4 AGTACTT 14695 0.0 24.878477 12-13 GTACTTT 15105 0.0 24.538252 14-15 GCAGAGT 27110 0.0 23.812824 9 CATGGGA 12515 0.0 22.775679 4 ACTTTTT 18500 0.0 20.730492 16-17 >>END_MODULE