FastQCFastQC Report
Thu 26 May 2016
SRR1364616_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364616_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3559822
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99200.2786656186741921No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG140450.032.8728451
TACATGG150150.029.480382
ACATGGG143550.029.2587323
ACGCATA2853.6379788E-1227.1458267
CATGGGG87050.024.774064
CATGGGT25100.024.6840824
GTATAGG11200.023.4041041
ATATAGG10600.022.4814192
TAAGGTA6750.021.1818964
ATGGGTA15600.021.0036815
CATGGGA60050.020.9327754
GTATCAA154450.020.828751
CGCATAG3801.9463187E-1020.355078
GTGTAAG13500.019.8580271
AGTACAT112700.019.453312
GTATAGT11400.019.3354471
GAGTACA127000.018.9980341
GTATAGA10400.018.9033151
CAACGCA169950.018.6837225
TCAACGC170350.018.5699064
Produced by FastQC (version 0.11.5)