##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364616_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3559822 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.134863765660192 31.0 27.0 31.0 10.0 34.0 2 26.68605846022638 31.0 26.0 31.0 10.0 34.0 3 27.41467269992713 31.0 27.0 31.0 12.0 34.0 4 30.894824797419645 35.0 32.0 37.0 12.0 37.0 5 31.1656959252457 35.0 32.0 37.0 16.0 37.0 6 31.154249285497983 35.0 32.0 37.0 15.0 37.0 7 31.098357445962186 35.0 32.0 37.0 12.0 37.0 8 30.84592516142661 35.0 31.0 37.0 10.0 37.0 9 31.863413676301793 37.0 31.0 39.0 10.0 39.0 10-11 31.80488968268638 37.0 31.0 39.0 10.0 39.0 12-13 31.803667851931923 37.0 31.0 39.0 9.0 39.0 14-15 32.48435876850022 38.0 31.0 40.0 9.0 41.0 16-17 32.888778849054816 38.0 32.0 40.0 9.0 41.0 18-19 32.68799788304022 38.0 31.5 40.0 8.0 41.0 20-21 32.580437870208115 38.0 31.0 40.0 8.0 41.0 22-23 32.621584169096096 38.0 31.0 40.0 8.0 41.0 24-25 32.679024400658236 38.0 31.0 40.0 8.0 41.0 26-27 32.45129180616334 38.0 30.5 40.0 6.5 41.0 28-29 32.343464785598826 38.0 30.0 40.0 2.0 41.0 30-31 32.20603473993924 38.0 30.0 40.0 2.0 41.0 32-33 32.1025183281636 38.0 30.0 40.0 2.0 41.0 34-35 32.1463688352957 38.0 30.0 40.0 2.0 41.0 36-37 32.08789049003012 38.0 30.0 40.0 2.0 41.0 38-39 31.777278189752185 38.0 30.0 40.0 2.0 41.0 40-41 31.628250232736356 37.0 30.0 40.0 2.0 41.0 42-43 31.109863358336455 37.0 29.0 40.0 2.0 41.0 44-45 30.953119566090663 37.0 29.0 40.0 2.0 41.0 46-47 30.90582843748929 36.5 29.0 40.0 2.0 41.0 48-49 30.827695036437216 36.0 29.0 40.0 2.0 41.0 50-51 30.562554110851607 36.0 28.0 40.0 2.0 41.0 52-53 30.41860646403107 36.0 28.0 40.0 2.0 41.0 54-55 30.20883726770608 35.5 28.0 39.5 2.0 41.0 56-57 29.932556037914253 35.0 28.0 39.0 2.0 41.0 58-59 29.67565527152762 35.0 27.5 39.0 2.0 40.0 60-61 29.338390795944292 35.0 27.0 39.0 2.0 40.0 62-63 29.12553015291214 34.0 26.5 38.5 2.0 40.0 64-65 28.871907499869376 34.0 26.0 38.0 2.0 40.0 66-67 28.573228801889535 34.0 26.0 37.5 2.0 40.0 68-69 28.31491827400359 34.0 26.0 37.0 2.0 40.0 70-71 27.873149696810685 33.5 26.0 36.5 2.0 39.0 72-73 27.644532788437175 33.0 26.0 36.0 2.0 39.0 74-75 27.624121515064516 33.0 26.0 36.0 2.0 39.0 76-77 27.400903331683438 33.0 26.0 35.0 2.0 38.0 78-79 27.127067589334523 33.0 25.5 35.0 2.0 37.0 80-81 26.735452783875147 33.0 25.0 35.0 2.0 37.0 82-83 26.91096563255129 33.0 25.5 35.0 2.0 36.0 84-85 26.783894672261702 33.0 25.0 35.0 2.0 36.0 86-87 26.69619028704244 33.0 25.0 35.0 2.0 36.0 88-89 26.55043328009097 33.0 25.0 35.0 2.0 35.0 90-91 26.433838967229264 33.0 25.0 35.0 2.0 35.0 92-93 26.324170000634865 33.0 25.0 35.0 2.0 35.0 94-95 26.227207146874196 33.0 24.5 35.0 2.0 35.0 96-97 26.11875973012134 33.0 24.0 35.0 2.0 35.0 98-99 26.03837031739227 33.0 24.0 35.0 2.0 35.0 100-101 25.93542893436807 33.0 24.0 35.0 2.0 35.0 102-103 24.71877947829976 31.0 19.5 34.0 2.0 35.0 104-105 25.610108033491564 32.5 23.0 35.0 2.0 35.0 106-107 25.78378455439626 33.0 23.0 35.0 2.0 35.0 108-109 25.743033078620222 33.0 23.0 35.0 2.0 35.0 110-111 25.669618874202136 33.0 23.0 35.0 2.0 35.0 112-113 25.570194240049084 33.0 21.0 35.0 2.0 35.0 114-115 25.45194506916357 33.0 20.0 35.0 2.0 35.0 116-117 25.32966170780449 33.0 19.0 35.0 2.0 35.0 118-119 25.184966832611295 33.0 18.0 35.0 2.0 35.0 120-121 24.978094269882035 33.0 12.0 35.0 2.0 35.0 122-123 24.747692440801814 33.0 2.0 35.0 2.0 35.0 124-125 23.71251104690066 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.604144843729511 1101 2 1.8958975021821445 1101 3 1.4746377150274341 1101 4 1.5065783734497842 1101 5 1.4275578620683866 1101 6 1.241667575850144 1101 7 1.2206532214272983 1101 8 1.473426418651158 1101 9 1.8155463326226418 1101 10-11 1.8309316448924449 1101 12-13 1.7963067640095431 1101 14-15 2.1721312852833634 1101 16-17 1.760287480421944 1101 18-19 1.798715350991344 1101 20-21 1.9683526260247248 1101 22-23 1.7527109011603912 1101 24-25 1.8107209250633147 1101 26-27 2.021878519891544 1101 28-29 2.0250238843728994 1101 30-31 2.1886338622224173 1101 32-33 2.1132787473787644 1101 34-35 1.9634656233233727 1101 36-37 1.8586194367851618 1101 38-39 1.8065120601963507 1101 40-41 1.8720035785879396 1101 42-43 2.157766504473905 1101 44-45 1.9323195920717566 1101 46-47 1.7514548764269406 1101 48-49 1.5054612518048138 1101 50-51 1.6308841628973916 1101 52-53 1.5009975995818507 1101 54-55 1.4455852451435014 1101 56-57 1.6061691268836498 1101 58-59 1.4808864147718204 1101 60-61 1.6179700984467118 1101 62-63 1.4111911889045388 1101 64-65 1.3787136123706212 1101 66-67 1.2964728318717498 1101 68-69 1.2045474759588473 1101 70-71 1.153225350110933 1101 72-73 1.082146451455749 1101 74-75 1.0840150482851314 1101 76-77 1.1860862481227912 1101 78-79 1.3146218313512854 1101 80-81 1.3525022703964389 1101 82-83 1.380388205589572 1101 84-85 1.3720723627244027 1101 86-87 1.3241552096049176 1101 88-89 1.3214597864102302 1101 90-91 1.3720596972171926 1101 92-93 1.4211935369838322 1101 94-95 1.3696946294971752 1101 96-97 1.383837660079294 1101 98-99 1.4328086727822935 1101 100-101 1.3604942145530607 1101 102-103 1.1944702151363558 1101 104-105 1.3472435563644076 1101 106-107 1.4922448765419603 1101 108-109 1.484960989846364 1101 110-111 1.4690376116964856 1101 112-113 1.420241963310879 1101 114-115 1.388945042072514 1101 116-117 1.4181259328167215 1101 118-119 1.3236768511582362 1101 120-121 1.303023356689728 1101 122-123 1.278390107410825 1101 124-125 1.1399874099795824 1102 1 1.0299587064177196 1102 2 1.3568178607799517 1102 3 0.9276305180064277 1102 4 0.8611286532234175 1102 5 0.790327542386251 1102 6 0.495930658371293 1102 7 0.6896618330046351 1102 8 0.6287922655611524 1102 9 1.0869657301287 1102 10-11 1.1345432122812653 1102 12-13 1.2754748631058916 1102 14-15 1.4976849506245102 1102 16-17 1.1043202391015683 1102 18-19 0.8819943258412835 1102 20-21 1.2901655476043743 1102 22-23 1.3081515629456177 1102 24-25 0.9621219271883987 1102 26-27 1.4418157889659469 1102 28-29 0.8852366392873705 1102 30-31 1.0098825346065112 1102 32-33 1.299158484049208 1102 34-35 1.1972282183813974 1102 36-37 1.01389442801878 1102 38-39 1.3013191446966523 1102 40-41 1.2039132350598045 1102 42-43 1.5064125035237552 1102 44-45 1.00980845257628 1102 46-47 0.9201454569683136 1102 48-49 0.9695399483007385 1102 50-51 1.0218618361638114 1102 52-53 0.8930632985077729 1102 54-55 0.8095597397271597 1102 56-57 0.8508467366198182 1102 58-59 0.8520230818550303 1102 60-61 0.8732137857042588 1102 62-63 0.6422664705363346 1102 64-65 0.4836144659329378 1102 66-67 0.5082694576611999 1102 68-69 0.45237383889797655 1102 70-71 0.6624149026485355 1102 72-73 0.22093473620944692 1102 74-75 0.3888036885767683 1102 76-77 0.24111972312273622 1102 78-79 0.4045919903975488 1102 80-81 0.48487701967980357 1102 82-83 0.5443656117504325 1102 84-85 0.42968310799955844 1102 86-87 0.4510959430945114 1102 88-89 0.35439757323901944 1102 90-91 0.4699805598252844 1102 92-93 0.513509504959373 1102 94-95 0.5264085185704275 1102 96-97 0.28295085876514037 1102 98-99 0.3856188059390284 1102 100-101 0.4566586184781549 1102 102-103 0.3977934278755342 1102 104-105 0.4429714045515176 1102 106-107 0.45905056348519935 1102 108-109 0.5435199508220059 1102 110-111 0.4282985159600301 1102 112-113 0.33839128888290304 1102 114-115 0.46349125648865197 1102 116-117 0.606191052320689 1102 118-119 0.581098940697732 1102 120-121 0.5763937460218536 1102 122-123 0.38294696083859137 1102 124-125 0.28964962181255416 1103 1 1.0347786780898574 1103 2 1.2806402638143446 1103 3 0.945075355071932 1103 4 0.6907902504599264 1103 5 0.8944706793236641 1103 6 0.8593583260287758 1103 7 0.9235386555451299 1103 8 1.1680164238715136 1103 9 1.5106507289759072 1103 10-11 1.558787101336371 1103 12-13 1.4488470378726248 1103 14-15 1.7917115650872049 1103 16-17 1.3630258540532978 1103 18-19 1.3514652950765793 1103 20-21 1.6595021429560717 1103 22-23 1.485039802611226 1103 24-25 1.4184071692996199 1103 26-27 1.6826664718521585 1103 28-29 1.5309979656402959 1103 30-31 1.6236851475679188 1103 32-33 1.6935388590730085 1103 34-35 1.496915366918671 1103 36-37 1.334985450488844 1103 38-39 1.417844996563364 1103 40-41 1.6642555242789179 1103 42-43 1.8298628181368102 1103 44-45 1.5847879843359607 1103 46-47 1.4079232568547297 1103 48-49 1.2464462054942835 1103 50-51 1.3343368293136812 1103 52-53 1.0934001003217197 1103 54-55 1.2442945397882745 1103 56-57 1.1893406282762449 1103 58-59 1.2983392458411842 1103 60-61 1.255361902197162 1103 62-63 1.0327434129381174 1103 64-65 0.889401706857953 1103 66-67 0.8833911946312121 1103 68-69 0.9492395192312557 1103 70-71 1.0614378853106352 1103 72-73 0.9351211572000295 1103 74-75 0.9366453000849084 1103 76-77 0.9404047540254439 1103 78-79 0.9078376280838611 1103 80-81 1.01079763412314 1103 82-83 0.9899189059368645 1103 84-85 0.9641819824973403 1103 86-87 0.877414348775126 1103 88-89 0.9122094859465584 1103 90-91 0.9887781171085201 1103 92-93 1.0276262654772204 1103 94-95 1.0250336004662053 1103 96-97 0.9999240820169177 1103 98-99 0.9788837736763369 1103 100-101 0.8488794093180196 1103 102-103 0.8605747281276876 1103 104-105 0.8827490678917265 1103 106-107 0.9127882147699111 1103 108-109 0.9969833413775717 1103 110-111 0.8853175661643107 1103 112-113 0.9550828034304573 1103 114-115 0.95643349689432 1103 116-117 0.9479312388377039 1103 118-119 0.9602507441208452 1103 120-121 0.9489874065902484 1103 122-123 0.8982849154457391 1103 124-125 0.8865492378950002 1104 1 1.2590499858476 1104 2 1.5858795295662382 1104 3 1.0785839481113548 1104 4 1.2163897977164737 1104 5 0.8839091363811953 1104 6 0.8606782629717102 1104 7 0.6259000055096458 1104 8 1.0759779677882584 1104 9 1.3720701991612962 1104 10-11 1.4576519456814552 1104 12-13 1.4074323042388812 1104 14-15 1.4945460230662135 1104 16-17 1.2582584184565277 1104 18-19 1.3941423913819264 1104 20-21 1.6563751822626713 1104 22-23 1.5239405597464284 1104 24-25 1.4580876853966558 1104 26-27 1.6389030185876123 1104 28-29 1.719956441776759 1104 30-31 1.793114755625858 1104 32-33 1.6756460745800368 1104 34-35 1.5758794155103644 1104 36-37 1.4638622622339739 1104 38-39 1.603741128624268 1104 40-41 1.5398992459334728 1104 42-43 1.8849169884308452 1104 44-45 1.7094664985709969 1104 46-47 1.5076389649162465 1104 48-49 1.2998168313763934 1104 50-51 1.2308486121301137 1104 52-53 1.1443076522799416 1104 54-55 1.2614972978747403 1104 56-57 1.2124762897093113 1104 58-59 1.1037223413714798 1104 60-61 1.0917663398850337 1104 62-63 1.0889879162896037 1104 64-65 1.0054675195803924 1104 66-67 0.9732575898457227 1104 68-69 0.9533966746118239 1104 70-71 1.0177882921394001 1104 72-73 0.8984533534032622 1104 74-75 0.8623807558038017 1104 76-77 0.8870864780172809 1104 78-79 0.8894311052966302 1104 80-81 1.0363129909215978 1104 82-83 1.0828582705216512 1104 84-85 1.0776284482736926 1104 86-87 0.994042013667876 1104 88-89 1.0268049838009219 1104 90-91 0.9985536529922605 1104 92-93 1.061227383300583 1104 94-95 1.0591344954407802 1104 96-97 1.0266612065071747 1104 98-99 1.0000258156518242 1104 100-101 0.9805072192936954 1104 102-103 0.9165420030671783 1104 104-105 1.0709861359373143 1104 106-107 1.0074491241977128 1104 108-109 1.0111320861843254 1104 110-111 1.0275133083792696 1104 112-113 0.9783245942606946 1104 114-115 1.0494946640684724 1104 116-117 0.9863761496498249 1104 118-119 1.040147329998323 1104 120-121 1.1197437644877297 1104 122-123 1.1241625665538457 1104 124-125 0.8272176275302741 1105 1 1.14019112705936 1105 2 1.547190862994512 1105 3 1.026997410910088 1105 4 1.2185217134568518 1105 5 0.8917650662276948 1105 6 0.9303915998965664 1105 7 0.8705919340538983 1105 8 0.8939154344981475 1105 9 1.119561419148841 1105 10-11 1.0888143379228197 1105 12-13 1.2397936868332877 1105 14-15 1.5038768850321773 1105 16-17 1.0187789057977952 1105 18-19 1.1895375301561586 1105 20-21 1.2162744686361506 1105 22-23 1.2507622225584 1105 24-25 1.0072344695262458 1105 26-27 1.3290482573575417 1105 28-29 1.398085649835096 1105 30-31 1.2730062325485605 1105 32-33 1.4005421416559685 1105 34-35 1.145294408037401 1105 36-37 0.8049703023301831 1105 38-39 1.0688290241968907 1105 40-41 1.1773472915753551 1105 42-43 1.393356361923523 1105 44-45 1.1125065750733185 1105 46-47 0.6508448291906106 1105 48-49 0.8006705901941835 1105 50-51 1.0517798976088741 1105 52-53 0.6842393225251016 1105 54-55 0.8516509263920788 1105 56-57 0.879886073814756 1105 58-59 0.9251977327483587 1105 60-61 0.8656873564651235 1105 62-63 0.7610323614779588 1105 64-65 0.5434069879332313 1105 66-67 0.5079242094338916 1105 68-69 0.24751398499851973 1105 70-71 0.6565134918074378 1105 72-73 0.5313816560605318 1105 74-75 0.6102855322398604 1105 76-77 0.6419736960883995 1105 78-79 0.6279438257929186 1105 80-81 0.8021183101874527 1105 82-83 0.7988050604334553 1105 84-85 0.6640825647589352 1105 86-87 0.7205306284071469 1105 88-89 0.8275941318805167 1105 90-91 0.553410272347346 1105 92-93 0.7220996313787076 1105 94-95 0.5747352584167196 1105 96-97 0.624364622088855 1105 98-99 0.729704384578099 1105 100-101 0.74416537187685 1105 102-103 0.6474126354445637 1105 104-105 0.692423147505135 1105 106-107 0.7226957194853583 1105 108-109 0.7282077224892589 1105 110-111 0.7629154764200301 1105 112-113 0.7414107846327092 1105 114-115 0.8117845892544722 1105 116-117 0.7913258135861483 1105 118-119 0.8437024145691225 1105 120-121 0.789970183228256 1105 122-123 0.7970742750792219 1105 124-125 0.7412582675220598 1106 1 0.44386995930756257 1106 2 -2.1118578804270065 1106 3 -0.17476131536837158 1106 4 0.4583321309834396 1106 5 0.6345917546864435 1106 6 0.5481666082410186 1106 7 0.7999001367764649 1106 8 0.790716968002851 1106 9 1.275225053746265 1106 10-11 1.4837631031619196 1106 12-13 1.4246759390842243 1106 14-15 1.5434365509995374 1106 16-17 1.121100489731667 1106 18-19 1.0979251217577612 1106 20-21 1.4715891742628031 1106 22-23 1.1515962009378526 1106 24-25 1.2148704900472893 1106 26-27 1.138494009980576 1106 28-29 1.2708156461832658 1106 30-31 1.3029985502024815 1106 32-33 1.3742802001527323 1106 34-35 1.2835619670848786 1106 36-37 1.1233788766419508 1106 38-39 1.4254889881144557 1106 40-41 1.444298580573939 1106 42-43 1.6854952619622168 1106 44-45 1.514835702264925 1106 46-47 1.2215750703477823 1106 48-49 1.1169212987071013 1106 50-51 1.2135571581690705 1106 52-53 1.2142633665322435 1106 54-55 1.2093226648645903 1106 56-57 1.2878683464483949 1106 58-59 1.2521633867628452 1106 60-61 1.3626691058085214 1106 62-63 1.1236634619607493 1106 64-65 1.295628907124133 1106 66-67 1.123410453276314 1106 68-69 0.9985764117339073 1106 70-71 1.0474784592467223 1106 72-73 0.9171659215287882 1106 74-75 0.9582820866394535 1106 76-77 0.8960184338674679 1106 78-79 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-1.9002863673803212 2216 82-83 -2.0518483210059237 2216 84-85 -1.9302014544911685 2216 86-87 -2.053800135563506 2216 88-89 -2.0309601306730016 2216 90-91 -1.9472519544492464 2216 92-93 -1.904381864121472 2216 94-95 -2.1674392637710582 2216 96-97 -2.164818101371253 2216 98-99 -2.128784234959621 2216 100-101 -2.1614997864522216 2216 102-103 -1.924307811286397 2216 104-105 -2.0049126515824938 2216 106-107 -1.960350911057077 2216 108-109 -2.118755800799189 2216 110-111 -2.003089935823123 2216 112-113 -2.1535698057145147 2216 114-115 -2.1313499471560604 2216 116-117 -1.9768307939176246 2216 118-119 -2.0981378891020483 2216 120-121 -2.254022825962892 2216 122-123 -2.020868972256647 2216 124-125 -1.8352549026915632 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 257609.0 3 37689.0 4 24346.0 5 20970.0 6 21234.0 7 22471.0 8 24551.0 9 25706.0 10 27322.0 11 29392.0 12 29084.0 13 27933.0 14 27243.0 15 23687.0 16 20418.0 17 18354.0 18 16742.0 19 16219.0 20 16591.0 21 17998.0 22 22600.0 23 31693.0 24 44975.0 25 56981.0 26 66488.0 27 74285.0 28 86205.0 29 98041.0 30 114209.0 31 134179.0 32 162795.0 33 204877.0 34 268315.0 35 366723.0 36 514451.0 37 501807.0 38 105592.0 39 47.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.17481704786738 18.723504527843577 13.913990397581996 24.18768802670704 2 13.897005375515844 21.178862143520387 39.710972028560896 25.21316045240287 3 18.635555043334207 25.2576565741841 28.522297455515407 27.584490926966282 4 12.421858183379559 16.87942127440893 36.30178321682262 34.39693732538889 5 14.120967530551349 38.32261897615108 33.05568731833295 14.50072617496462 6 32.77572336763566 36.23528934705427 16.795938688341412 14.19304859696866 7 28.98197988416826 32.481372118109064 20.5724798685412 17.96416812918147 8 25.495410661678285 35.73290768821495 19.656950303398826 19.114731346707934 9 25.67228615295745 17.360136385568488 19.832265569006065 37.135311892467996 10-11 24.490445004212326 27.04392058460811 27.230208222117096 21.23542618906247 12-13 25.080790509473736 24.270514499798157 27.270235477583178 23.37845951314493 14-15 22.624634151249897 25.90578570526886 24.597152976531497 26.87242716694975 16-17 23.144693251702055 28.786576535725246 24.8949137509808 23.173816461591905 18-19 22.96621749991204 27.813446856419098 26.317742673187205 22.902592970481653 20-21 23.743825921343024 26.916485097847335 26.19899098256278 23.140697998246864 22-23 23.67793425247556 26.37078169316414 26.12029080365123 23.830993250709064 24-25 23.795738435485976 26.766203380517663 25.969686358579875 23.468371825416483 26-27 23.28885465526443 26.795563772877113 26.449244036144325 23.466337535714135 28-29 23.216949689632045 26.907228448092695 26.48191304969405 23.393908812581206 30-31 23.349676298985813 26.734683340796483 26.29302795326618 23.62261240695152 32-33 23.293576938363515 26.786208888273382 26.212367380165034 23.707846793198065 34-35 23.736648951787174 26.755779868061968 26.080657121349198 23.426914058801657 36-37 23.91863151441248 26.421222128217025 25.881265902063966 23.77888045530653 38-39 23.4317371734539 26.82406477557766 26.07885179164096 23.665346259327478 40-41 23.395104546654828 26.642506011777513 26.531996844766926 23.430392596800736 42-43 23.50197227060074 26.644196186168823 25.94977101096739 23.904060532263053 44-45 23.451390938923524 26.545161719708354 25.98631250698262 24.0171348343855 46-47 23.733696526103916 26.70519188680023 25.73779862356468 23.823312963531173 48-49 23.673201501729437 26.70901916977182 25.72337029504889 23.894409033449847 50-51 23.467964271317935 26.74220237764432 25.842710224686616 23.947123126351133 52-53 23.818982046672318 26.665620591267146 25.61469477935666 23.90070258270387 54-55 23.82332331487235 26.445514768787426 25.67213742176595 24.05902449457427 56-57 23.72621049998157 26.81272127763218 25.411473328772587 24.04959489361367 58-59 23.877031544638594 26.325512761268932 25.66348962520476 24.13396606888771 60-61 23.840092202010982 26.805086924059257 25.763241904643458 23.591578969286303 62-63 23.576111652152335 26.796930136940244 25.885598829944236 23.741359380963186 64-65 23.578624226718492 26.65066475588998 25.63054294387614 24.14016807351539 66-67 23.654479685637973 26.84272714320454 25.298497795856807 24.204295375300685 68-69 23.556756907291927 26.488216424471737 25.87340038755127 24.08162628068507 70-71 23.7781351952172 26.288626225360055 25.92921306197426 24.00402551744848 72-73 23.68509864238581 26.408567259035642 25.479670379273077 24.42666371930547 74-75 23.429778836306355 26.995703622060486 25.41819480128848 24.15632274034468 76-77 23.67147332707148 26.605105856519202 25.580803626810518 24.1426171895988 78-79 23.47458797720248 26.684023533469475 25.71842884017513 24.122959649152914 80-81 23.754676999523245 26.941655551971404 25.591193700686382 23.712473747818972 82-83 23.918494808293957 26.434434849009413 25.760228418465758 23.886841924230872 84-85 23.4498553438383 26.668750330554243 25.590187012116004 24.29120731349145 86-87 23.557350703628067 26.645792371962035 25.873741752500877 23.923115171909018 88-89 23.61996378018693 26.71654394550595 25.478916422049743 24.184575852257378 90-91 23.514537367221383 26.75110778678556 25.658000521344526 24.076354324648523 92-93 23.64252830325639 26.66556564450733 25.689050897328368 24.00285515490791 94-95 23.651697321693526 26.27674637550547 25.935730043839932 24.13582625896107 96-97 23.64059146112158 26.25817595069366 25.88633559134083 24.21489699684393 98-99 23.380769435838307 26.659598871685052 25.68772078946032 24.27191090301632 100-101 23.219603392994483 26.366793122868053 25.994443091052403 24.41916039308506 102-103 23.55897588586458 25.918368725328158 26.091105036791596 24.431550352015666 104-105 23.376446928028038 26.345725169012503 25.938593050301005 24.339234852658453 106-107 23.296485818934716 26.364614570967216 26.153994384962047 24.184905225136017 108-109 23.29745035000961 26.332789489780218 25.796964323243955 24.572795836966225 110-111 22.933264628027995 26.608178087751718 25.925883214294455 24.532674069925836 112-113 23.028277869214854 26.434705864485373 26.278851897809975 24.258164368489794 114-115 22.95063271576944 26.793585277801096 25.78384434189446 24.471937664534998 116-117 22.87236870045549 26.66291662389274 26.255690103965247 24.20902457168652 118-119 22.947219529273312 26.306168489474768 26.08104811524431 24.665563866007613 120-121 22.704510047423497 26.795364754322502 26.017673410126278 24.48245178812772 122-123 22.820276051817164 26.552162137760394 25.952684787167048 24.674877023255394 124-125 22.892075236504535 26.621204469774344 25.89273773467813 24.59398255904299 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11785.0 1 8679.0 2 4408.0 3 2596.5 4 1609.5 5 1177.0 6 1049.0 7 1082.0 8 1198.5 9 1186.5 10 1092.0 11 1035.0 12 1098.0 13 1175.0 14 1224.0 15 1339.5 16 1447.5 17 1587.5 18 1812.5 19 1991.0 20 2274.0 21 2742.0 22 3411.5 23 4313.5 24 5367.0 25 6871.5 26 9240.0 27 11881.5 28 13881.0 29 17640.0 30 24053.5 31 31596.5 32 41573.5 33 50632.0 34 58497.5 35 67513.5 36 79297.0 37 90027.0 38 94689.0 39 95787.5 40 94692.5 41 94937.5 42 94912.0 43 101579.0 44 112490.5 45 117352.0 46 118281.0 47 120436.5 48 130220.5 49 133937.0 50 130125.5 51 129700.0 52 132964.0 53 138336.0 54 140210.5 55 134826.0 56 125544.5 57 117542.0 58 112090.0 59 102860.5 60 87450.0 61 71766.0 62 57840.5 63 45860.5 64 36855.0 65 29661.5 66 22460.0 67 17501.0 68 13279.0 69 9321.5 70 7072.0 71 5545.5 72 4385.5 73 3508.0 74 2757.5 75 2163.5 76 1524.0 77 1025.0 78 735.5 79 503.0 80 372.0 81 287.5 82 224.5 83 169.5 84 136.0 85 106.0 86 71.0 87 59.5 88 47.0 89 32.5 90 23.5 91 16.5 92 17.0 93 13.0 94 7.0 95 5.0 96 3.0 97 3.0 98 2.5 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2191120791994656 2 0.2554341200206078 3 0.30925703588550213 4 0.2951271159063571 5 0.3308873308833981 6 0.2431020427425866 7 0.2329049036721499 8 0.21256680811568668 9 0.21180834322615005 10-11 0.1509204673716832 12-13 0.17814092951838603 14-15 0.19533280034788256 16-17 0.167297690727233 18-19 0.19515020694854968 20-21 0.1899392722445111 22-23 0.21340954688183847 24-25 0.20310004264258155 26-27 0.25442283350122563 28-29 0.18144165635248055 30-31 0.15993777216950733 32-33 0.20186402578555893 34-35 0.15881412048130497 36-37 0.15857534449756197 38-39 0.19321190778640054 40-41 0.2142803769401953 42-43 0.2803370505603932 44-45 0.17863252713197458 46-47 0.1625502623445779 48-49 0.16628640420785085 50-51 0.17120238034373628 52-53 0.19269221888060695 54-55 0.17927862685269094 56-57 0.1772841451061317 58-59 0.1521003016442957 60-61 0.16535939156508386 62-63 0.1534205923779335 64-65 0.14738096455384567 66-67 0.13518934373684977 68-69 0.1387007552624822 70-71 0.1404424153791959 72-73 0.11514620674853969 74-75 0.11664909088151038 76-77 0.13941708321371124 78-79 0.14677700177143688 80-81 0.15712864294900136 82-83 0.15853320755925435 84-85 0.14568144137543956 86-87 0.17329518161301324 88-89 0.16759264929538611 90-91 0.1559207173841838 92-93 0.1558785804458762 94-95 0.17771956013531012 96-97 0.1651065699352383 98-99 0.1593478550332011 100-101 0.13955753967473655 102-103 0.18059891758632876 104-105 0.13577926087315603 106-107 0.14275994698611336 108-109 0.15424928549798278 110-111 0.12756255790317605 112-113 0.11938799187150369 114-115 0.11678954734253566 116-117 0.12098919552719209 118-119 0.10462601781774483 120-121 0.09726609926001918 122-123 0.12570853261764212 124-125 0.1219302538160616 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3559822.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.212762296062564 #Duplication Level Percentage of deduplicated Percentage of total 1 71.93458391358001 27.488191559557997 2 10.771937522832072 8.23250976056038 3 5.023016917814164 5.758300544686006 4 3.0199581551612047 4.61603772508923 5 1.9838280134809265 3.790377415770832 6 1.3399289957416647 3.072143292472684 7 1.0021989829859865 2.6807754057139412 8 0.7511651849687274 2.296327732663028 9 0.587502747422739 2.020509255199393 >10 3.2432513252715927 22.031309286381966 >50 0.200772440093002 5.303284242279391 >100 0.1316207934188505 9.102925292584766 >500 0.007715620834534464 1.985083180641652 >1k 0.0024406555696195997 1.3237798947878279 >5k 7.873082482643869E-5 0.2984454116109918 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9920 0.2786656186741921 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.809129220505969E-5 2 0.0 2.809129220505969E-5 0.0 0.0 5.618258441011938E-5 3 0.0 2.809129220505969E-5 0.0 0.0 5.618258441011938E-5 4 0.0 2.809129220505969E-5 0.0 0.0 1.1236516882023877E-4 5 0.0 2.809129220505969E-5 0.0 0.0 1.1236516882023877E-4 6 0.0 2.809129220505969E-5 0.0 0.0 3.370955064607163E-4 7 0.0 2.809129220505969E-5 0.0 0.0 3.370955064607163E-4 8 0.0 2.809129220505969E-5 0.0 0.0 3.6518679866577597E-4 9 0.0 2.809129220505969E-5 0.0 0.0 4.4946067528095506E-4 10-11 0.0 2.809129220505969E-5 0.0 0.0 4.4946067528095506E-4 12-13 0.0 2.809129220505969E-5 0.0 0.0 4.635063213834849E-4 14-15 0.0 2.809129220505969E-5 0.0 0.0 7.303735973315519E-4 16-17 0.0 2.809129220505969E-5 0.0 0.0 8.989213505619101E-4 18-19 0.0 2.809129220505969E-5 0.0 0.0 0.0011096060420998578 20-21 0.0 2.809129220505969E-5 0.0 2.809129220505969E-5 0.0011517429804074474 22-23 0.0 2.809129220505969E-5 0.0 2.5282162984553724E-4 0.001530975425175753 24-25 0.0 2.809129220505969E-5 0.0 6.460997207163729E-4 0.001924253516046589 26-27 0.0 2.809129220505969E-5 0.0 0.0013905189641504547 0.0019663904543541786 28-29 0.0 2.809129220505969E-5 0.0 0.0033147724801970433 0.0019663904543541786 30-31 0.0 2.809129220505969E-5 0.0 0.007036868697367453 0.0019804361004567084 32-33 0.0 2.809129220505969E-5 0.0 0.013301226859095763 0.0022332577303022455 34-35 0.0 2.809129220505969E-5 0.0 0.0208016018778467 0.0023315772530199544 36-37 0.0 2.809129220505969E-5 0.0 0.0335550485389438 0.002359668545225014 38-39 0.0 2.809129220505969E-5 0.0 0.053766733280484244 0.002457988067942723 40-41 0.0 2.809129220505969E-5 0.0 0.07720891662560656 0.0025001250062503125 42-43 0.0 2.809129220505969E-5 0.0 0.09869875516247723 0.002542261944557902 44-45 0.0 2.809129220505969E-5 0.0 0.12416351154636385 0.0026546271133781408 46-47 0.0 2.809129220505969E-5 0.0 0.15565385010823574 0.002907448743223678 48-49 0.0 2.809129220505969E-5 0.0 0.18967240496856302 0.0031743160191717453 50-51 0.0 2.809129220505969E-5 0.0 0.22532025477678377 0.003188361665274275 52-53 0.0 2.809129220505969E-5 0.0 0.2615158847830032 0.003483320233427402 54-55 2.809129220505969E-5 2.809129220505969E-5 0.0 0.3005627809480362 0.0037782788015805284 56-57 2.809129220505969E-5 2.809129220505969E-5 0.0 0.34939949244653246 0.0037923244476830583 58-59 2.809129220505969E-5 2.809129220505969E-5 0.0 0.404261786123014 0.0038485070320931777 60-61 2.809129220505969E-5 2.809129220505969E-5 1.4045646102529846E-5 0.4617084786823611 0.0039889634931184765 62-63 2.809129220505969E-5 2.809129220505969E-5 2.809129220505969E-5 0.5198995904851422 0.004157511246348835 64-65 2.809129220505969E-5 2.809129220505969E-5 2.809129220505969E-5 0.5818689810895039 0.004157511246348835 66-67 2.809129220505969E-5 2.809129220505969E-5 2.809129220505969E-5 0.6568727312770133 0.004241785122964014 68-69 2.809129220505969E-5 2.809129220505969E-5 2.809129220505969E-5 0.7427197202556757 0.004326058999579193 70-71 2.809129220505969E-5 2.809129220505969E-5 2.809129220505969E-5 0.8402667324377455 0.004466515460604491 72-73 2.809129220505969E-5 5.618258441011938E-5 2.809129220505969E-5 0.9394149482755036 0.005365436811166401 74-75 2.809129220505969E-5 5.618258441011938E-5 2.809129220505969E-5 1.0449820243821182 0.0053935281033714606 76-77 2.809129220505969E-5 5.618258441011938E-5 2.809129220505969E-5 1.1619260738317814 0.00543566504167905 78-79 2.809129220505969E-5 5.618258441011938E-5 2.809129220505969E-5 1.2999385924352396 0.00547780197998664 80-81 2.809129220505969E-5 5.618258441011938E-5 2.809129220505969E-5 1.455367712205835 0.005702532317627117 82-83 2.809129220505969E-5 5.618258441011938E-5 2.809129220505969E-5 1.6174544682290295 0.0061941299312156615 84-85 2.809129220505969E-5 5.618258441011938E-5 2.809129220505969E-5 1.7889939440792264 0.006208175577318191 86-87 2.809129220505969E-5 5.618258441011938E-5 2.809129220505969E-5 1.97766910817451 0.006236266869523252 88-89 2.809129220505969E-5 5.618258441011938E-5 2.809129220505969E-5 2.1914297962089115 0.006250312515625782 90-91 2.809129220505969E-5 7.022823051264923E-5 2.809129220505969E-5 2.4325233115588363 0.006292449453933371 92-93 5.618258441011938E-5 8.427387661517907E-5 2.809129220505969E-5 2.696721914747423 0.006292449453933371 94-95 5.618258441011938E-5 8.427387661517907E-5 2.809129220505969E-5 2.9655696267959466 0.006657636252599147 96-97 5.618258441011938E-5 8.427387661517907E-5 2.809129220505969E-5 3.253673919651039 0.006657636252599147 98-99 5.618258441011938E-5 8.427387661517907E-5 2.809129220505969E-5 3.567299151474428 0.006755955775316856 100-101 5.618258441011938E-5 8.427387661517907E-5 2.809129220505969E-5 3.923904060371558 0.0067980927136244455 102-103 5.618258441011938E-5 8.427387661517907E-5 2.809129220505969E-5 4.307097377340777 0.007205416450597811 104-105 7.022823051264923E-5 8.427387661517907E-5 2.809129220505969E-5 4.69791748014367 0.007402055496033229 106-107 8.427387661517907E-5 8.427387661517907E-5 2.809129220505969E-5 5.101687668653096 0.007528466310955997 108-109 8.427387661517907E-5 8.427387661517907E-5 2.809129220505969E-5 5.531161389530151 0.0077531966485964755 110-111 8.427387661517907E-5 8.427387661517907E-5 2.809129220505969E-5 6.0040502025101254 0.008329068138800199 112-113 8.427387661517907E-5 8.427387661517907E-5 2.809129220505969E-5 6.510283379337507 0.008609981060850796 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14045 0.0 32.872845 1 TACATGG 15015 0.0 29.48038 2 ACATGGG 14355 0.0 29.258732 3 ACGCATA 285 3.6379788E-12 27.145826 7 CATGGGG 8705 0.0 24.77406 4 CATGGGT 2510 0.0 24.684082 4 GTATAGG 1120 0.0 23.404104 1 ATATAGG 1060 0.0 22.481419 2 TAAGGTA 675 0.0 21.181896 4 ATGGGTA 1560 0.0 21.003681 5 CATGGGA 6005 0.0 20.932775 4 GTATCAA 15445 0.0 20.82875 1 CGCATAG 380 1.9463187E-10 20.35507 8 GTGTAAG 1350 0.0 19.858027 1 AGTACAT 11270 0.0 19.45331 2 GTATAGT 1140 0.0 19.335447 1 GAGTACA 12700 0.0 18.998034 1 GTATAGA 1040 0.0 18.903315 1 CAACGCA 16995 0.0 18.683722 5 TCAACGC 17035 0.0 18.569906 4 >>END_MODULE