FastQCFastQC Report
Thu 26 May 2016
SRR1364616_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364616_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3559822
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111400.31293699516436496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89580.2516417955729247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63510.1784077967943341No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40060.11253371657346913No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG136950.030.6212021
GTATCAA201250.030.1020931
TACATGG151050.027.5107232
ACATGGG144700.027.3437443
TCAACGC238050.024.996514
GGTATCA151650.024.7069911
ATCAACG243300.024.5036333
CAACGCA239350.024.4424485
TATCAAC245950.024.4237122
GAGTACT126850.024.08941812-13
AACGCAG242700.024.0481916
GTACTTT127550.024.02726414-15
AGTACTT139550.022.4093312-13
ATATAGG11500.022.2606542
CATGGGG90750.022.1624434
AGAGTAC200600.022.10351810-11
CATGGGA58900.021.619524
ACGCAGA270400.021.3867477
TACTTTT145000.021.05354514-15
CGCAGAG274550.020.9767048
Produced by FastQC (version 0.11.5)