##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364616_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3559822 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.730092965322424 31.0 30.0 33.0 26.0 34.0 2 29.786323866755136 31.0 30.0 34.0 26.0 34.0 3 29.80611811489451 31.0 30.0 34.0 26.0 34.0 4 33.50776808503347 35.0 33.0 37.0 28.0 37.0 5 32.92747109265576 35.0 33.0 37.0 26.0 37.0 6 32.939082347375795 35.0 33.0 37.0 26.0 37.0 7 32.72229454169338 35.0 33.0 37.0 25.0 37.0 8 32.643661958378814 35.0 33.0 37.0 25.0 37.0 9 33.947311410514345 38.0 33.0 39.0 25.0 39.0 10-11 33.72803345223441 37.0 33.0 39.0 23.5 39.0 12-13 33.60421602540801 37.0 33.0 39.0 22.5 39.0 14-15 34.573001824248514 38.0 33.0 41.0 23.0 41.0 16-17 34.47130137405746 38.0 33.0 41.0 22.0 41.0 18-19 34.495628011737665 38.5 33.0 41.0 22.0 41.0 20-21 34.501504148241125 39.0 33.0 41.0 22.0 41.0 22-23 34.473958950756526 39.0 33.0 41.0 22.0 41.0 24-25 34.454394068018004 39.0 33.0 41.0 22.0 41.0 26-27 34.33349392188711 38.5 33.0 41.0 21.5 41.0 28-29 34.09546586879905 38.0 33.0 41.0 18.5 41.0 30-31 34.02367688609149 38.0 33.0 40.5 18.5 41.0 32-33 33.849287689103555 38.0 33.0 40.0 18.0 41.0 34-35 33.7063513288024 38.0 33.0 40.0 16.5 41.0 36-37 33.543052012151165 38.0 32.5 40.0 16.0 41.0 38-39 33.399692035163554 38.0 32.0 40.0 15.0 41.0 40-41 33.22108816114963 38.0 32.0 40.0 14.0 41.0 42-43 33.04727160515329 38.0 32.0 40.0 9.0 41.0 44-45 32.83711699629925 38.0 31.5 40.0 8.5 41.0 46-47 32.58048028805935 38.0 31.0 40.0 7.5 41.0 48-49 32.32952560549376 37.5 31.0 40.0 2.0 41.0 50-51 32.10220595299428 37.0 31.0 40.0 2.0 41.0 52-53 31.916708897242614 37.0 30.5 40.0 2.0 41.0 54-55 31.668904091271976 37.0 30.0 40.0 2.0 41.0 56-57 31.404812515906695 36.0 30.0 40.0 2.0 41.0 58-59 31.18893233987542 36.0 29.5 40.0 2.0 41.0 60-61 30.94005992434453 35.5 29.0 39.0 2.0 41.0 62-63 30.64693248707379 35.0 29.0 39.0 2.0 41.0 64-65 30.35856947341749 35.0 29.0 39.0 2.0 40.5 66-67 30.05916054229678 35.0 29.0 38.0 2.0 40.0 68-69 29.73617487054128 35.0 28.0 37.5 2.0 40.0 70-71 29.471971070463635 34.0 28.0 37.0 2.0 39.5 72-73 29.315014486679388 34.0 28.0 36.5 2.0 39.0 74-75 29.050046041627922 34.0 28.0 36.0 2.0 39.0 76-77 28.782702899189903 34.0 28.0 36.0 2.0 38.5 78-79 28.513484382084272 34.0 28.0 35.0 2.0 37.0 80-81 28.27199885275163 34.0 27.5 35.0 2.0 37.0 82-83 28.055036740601075 34.0 27.0 35.0 2.0 36.5 84-85 28.082794729624123 34.0 28.0 35.0 2.0 36.0 86-87 27.982998307218732 34.0 28.0 35.0 2.0 36.0 88-89 27.871197352002433 34.0 27.0 35.0 2.0 35.5 90-91 27.74309291307262 34.0 27.0 35.0 2.0 35.0 92-93 27.62211340904124 34.0 27.0 35.0 2.0 35.0 94-95 27.549949828952123 34.0 27.0 35.0 2.0 35.0 96-97 27.47260466955932 34.0 27.0 35.0 2.0 35.0 98-99 27.399088774663454 34.0 27.0 35.0 2.0 35.0 100-101 27.30498097938605 34.0 26.5 35.0 2.0 35.0 102-103 26.07435175129543 31.5 24.5 34.0 2.0 35.0 104-105 27.044159932715736 33.5 25.5 35.0 2.0 35.0 106-107 27.225024313013403 34.0 27.0 35.0 2.0 35.0 108-109 27.238923603483546 34.0 27.0 35.0 2.0 35.0 110-111 27.180013775969698 34.0 27.0 35.0 2.0 35.0 112-113 27.135793868345104 34.0 27.0 35.0 2.0 35.0 114-115 27.050529773679695 34.0 26.0 35.0 2.0 35.0 116-117 26.92855822566409 34.0 25.5 35.0 2.0 35.0 118-119 26.81504496573143 34.0 25.0 35.0 2.0 35.0 120-121 26.673731158468037 34.0 25.0 35.0 2.0 35.0 122-123 26.48217467053128 34.0 25.0 35.0 2.0 35.0 124-125 25.63458257182522 33.0 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7007170893582071 1101 2 0.7244226195422101 1101 3 0.5746653633290606 1101 4 0.689378024062222 1101 5 0.9440982663781625 1101 6 0.9343228429247716 1101 7 0.8370371775761996 1101 8 0.9839924444326584 1101 9 1.1123214139326763 1101 10-11 1.0993957916863124 1101 12-13 1.205588438397676 1101 14-15 1.3147055686017382 1101 16-17 1.3687633559342132 1101 18-19 1.3341374117060525 1101 20-21 1.4080090693106442 1101 22-23 1.2838404029033654 1101 24-25 1.2271213812963424 1101 26-27 1.256962904842787 1101 28-29 1.3345708696965133 1101 30-31 1.375430821328223 1101 32-33 1.451304197997338 1101 34-35 1.4559398224433409 1101 36-37 1.4843138178171884 1101 38-39 1.4984977285317314 1101 40-41 1.5482084931592368 1101 42-43 1.558743541268882 1101 44-45 1.4347609018197218 1101 46-47 1.5430892794046116 1101 48-49 1.6112829278648277 1101 50-51 1.5416508166683087 1101 52-53 1.4953246262970694 1101 54-55 1.5452678667057285 1101 56-57 1.5245546561601273 1101 58-59 1.539394186210039 1101 60-61 1.4634249960768422 1101 62-63 1.4583931553556013 1101 64-65 1.4636090465733957 1101 66-67 1.4724522612263904 1101 68-69 1.5233875788963722 1101 70-71 1.5146003415635505 1101 72-73 1.4506900110223633 1101 74-75 1.417404903490418 1101 76-77 1.4770693001880026 1101 78-79 1.4126250637167033 1101 80-81 1.3474316042577001 1101 82-83 1.3704956552834702 1101 84-85 1.3694647215136655 1101 86-87 1.299171776497893 1101 88-89 1.2940362450001572 1101 90-91 1.314715242083821 1101 92-93 1.3805985234003053 1101 94-95 1.3784285515917496 1101 96-97 1.4110688132094964 1101 98-99 1.5182010903353031 1101 100-101 1.5057607297012687 1101 102-103 1.4944493237371894 1101 104-105 1.48917024512064 1101 106-107 1.4592187173603648 1101 108-109 1.4338124065612874 1101 110-111 1.4468031497572547 1101 112-113 1.465153489389131 1101 114-115 1.4373414207436639 1101 116-117 1.4817834538224233 1101 118-119 1.5361971576952804 1101 120-121 1.5042488525976836 1101 122-123 1.543186814129637 1101 124-125 1.3312964852643603 1102 1 0.4899036724487438 1102 2 -0.4255980418053724 1102 3 0.17954621562185835 1102 4 0.3780078448700124 1102 5 0.7377767078126922 1102 6 0.7239794739333618 1102 7 0.6707689750193992 1102 8 0.6147918315185308 1102 9 0.9438012782061591 1102 10-11 1.0003233550813917 1102 12-13 1.149537502795802 1102 14-15 1.2307569852467992 1102 16-17 1.3246633416295026 1102 18-19 1.2588604999165298 1102 20-21 1.199185977646053 1102 22-23 1.1885834345135038 1102 24-25 1.198208840916294 1102 26-27 1.1894952952350124 1102 28-29 1.3242064143757517 1102 30-31 1.1780456165891948 1102 32-33 1.111526591859949 1102 34-35 0.9632409187697135 1102 36-37 0.9259623564495669 1102 38-39 0.8003903260546537 1102 40-41 0.8381052298237179 1102 42-43 0.7698890949920951 1102 44-45 0.6600528145613822 1102 46-47 0.6811734145820836 1102 48-49 0.7078414074355948 1102 50-51 0.6247836520435754 1102 52-53 0.6365501181863351 1102 54-55 0.7247911963058513 1102 56-57 0.5170641264718938 1102 58-59 0.7369048962026596 1102 60-61 0.5948255424768014 1102 62-63 0.7492589589536252 1102 64-65 0.6763923172656199 1102 66-67 0.643888242063511 1102 68-69 0.4817540140896348 1102 70-71 0.37630673558602723 1102 72-73 0.48934930024669043 1102 74-75 0.5948746488576155 1102 76-77 0.5787215388744009 1102 78-79 0.6718107371654156 1102 80-81 0.5723092230722031 1102 82-83 0.5190003433748842 1102 84-85 0.6547295366945214 1102 86-87 0.5860611070865076 1102 88-89 0.6755581101311989 1102 90-91 0.5732077612292485 1102 92-93 0.6687727095014466 1102 94-95 0.44792695917602643 1102 96-97 0.4482361632528722 1102 98-99 0.547270078258407 1102 100-101 0.3259234018708774 1102 102-103 0.3880500025506173 1102 104-105 0.3131839212147298 1102 106-107 0.6251019587740636 1102 108-109 0.7013919762591101 1102 110-111 0.5865503380507597 1102 112-113 0.5067150966448537 1102 114-115 0.6981518808650939 1102 116-117 0.7353953919400134 1102 118-119 0.6286581077483469 1102 120-121 0.6902058816966345 1102 122-123 0.7032289253016124 1102 124-125 0.542427860872472 1103 1 0.2840435034344715 1103 2 -0.9175091210151329 1103 3 0.04397266038754566 1103 4 0.3460463202330075 1103 5 0.5183972957317451 1103 6 0.6134294354977925 1103 7 0.5609762934216178 1103 8 0.6963190095994349 1103 9 0.7885701801354941 1103 10-11 0.9185352423051754 1103 12-13 0.9790414877362892 1103 14-15 1.024131664577709 1103 16-17 1.1171466188261618 1103 18-19 1.049770812240432 1103 20-21 1.1074600390323326 1103 22-23 1.083927354802647 1103 24-25 0.9032514486276213 1103 26-27 1.0268086463327464 1103 28-29 1.0570416704610537 1103 30-31 0.9088164509759835 1103 32-33 0.9039478573621906 1103 34-35 0.9218829338558763 1103 36-37 1.0693015757520925 1103 38-39 1.061188822001803 1103 40-41 1.16224654492801 1103 42-43 1.2014992869618197 1103 44-45 1.1993899520818516 1103 46-47 1.256892614238751 1103 48-49 1.2141484549542199 1103 50-51 1.246273104670351 1103 52-53 1.1392911920711626 1103 54-55 1.1588506037646766 1103 56-57 1.1431201918344485 1103 58-59 1.1627588622219989 1103 60-61 1.141416569601848 1103 62-63 1.2346961048895757 1103 64-65 1.155348447049505 1103 66-67 1.1838514110965797 1103 68-69 1.117174447718778 1103 70-71 1.177311007401336 1103 72-73 1.1219968600582177 1103 74-75 1.0079954982772712 1103 76-77 0.9019489253708208 1103 78-79 1.0259177203904954 1103 80-81 1.0825771511970572 1103 82-83 1.096709777056784 1103 84-85 1.0834831198915964 1103 86-87 1.0652979050486806 1103 88-89 1.019705398219422 1103 90-91 1.0771988353422621 1103 92-93 1.0753196803935587 1103 94-95 0.9937205418327615 1103 96-97 1.0459487769280464 1103 98-99 1.0825565396104437 1103 100-101 1.1302105695027116 1103 102-103 1.084782951935967 1103 104-105 1.0808601475156117 1103 106-107 1.0853319442121752 1103 108-109 1.05766986166946 1103 110-111 1.018716189121566 1103 112-113 1.0439249245400362 1103 114-115 0.9751535196021557 1103 116-117 0.9538017844932085 1103 118-119 0.9606418120170055 1103 120-121 0.9313459220653897 1103 122-123 0.9591756313889981 1103 124-125 0.9661565983183849 1104 1 0.34409477454094883 1104 2 -0.7763853333665551 1104 3 0.12396189106890532 1104 4 0.32009098445357154 1104 5 0.7460061109280787 1104 6 0.667708559377644 1104 7 0.8107157266013232 1104 8 0.9181262726837787 1104 9 0.8562211491588414 1104 10-11 1.0125251873169745 1104 12-13 0.8945919454252689 1104 14-15 0.9817832640587412 1104 16-17 1.1279563206450902 1104 18-19 1.0703711936355589 1104 20-21 1.1254148102944512 1104 22-23 1.0928369291674613 1104 24-25 1.0459540103402816 1104 26-27 0.9240011363603173 1104 28-29 1.2237730008121943 1104 30-31 1.1011402332871967 1104 32-33 1.2076878093104355 1104 34-35 1.190154946060069 1104 36-37 1.1298757454982677 1104 38-39 1.1592257687990184 1104 40-41 1.1528235499687725 1104 42-43 1.2225281571936506 1104 44-45 1.2416444899475394 1104 46-47 1.2335284030694496 1104 48-49 1.2843040989307397 1104 50-51 1.2441769564138099 1104 52-53 1.0414571672229371 1104 54-55 1.181981164350276 1104 56-57 1.1784718534363847 1104 58-59 1.0864560793055276 1104 60-61 1.1571587379653927 1104 62-63 1.231134001312789 1104 64-65 1.201591953529487 1104 66-67 1.1783996465104742 1104 68-69 1.1273107141229453 1104 70-71 1.1782446742865602 1104 72-73 1.1737875116768315 1104 74-75 1.1045030733357954 1104 76-77 1.1707645573043983 1104 78-79 1.0791383566472632 1104 80-81 1.0653752403101677 1104 82-83 1.167851508392431 1104 84-85 1.1438706939306655 1104 86-87 1.1785182214876073 1104 88-89 1.1506703098250881 1104 90-91 1.15233956048721 1104 92-93 1.1445377568367974 1104 94-95 1.0783811074387302 1104 96-97 1.1005444887003009 1104 98-99 1.081100927185048 1104 100-101 1.191709934138327 1104 102-103 1.2042225604789287 1104 104-105 1.1485339556275918 1104 106-107 1.1322983237323108 1104 108-109 1.0219796732360358 1104 110-111 0.9966811105406244 1104 112-113 0.9493521944683394 1104 114-115 1.069303955984072 1104 116-117 1.1161123391950127 1104 118-119 0.9874069293933303 1104 120-121 0.959770579865598 1104 122-123 1.0413649334751653 1104 124-125 1.0284409816132225 1105 1 0.5030714273194619 1105 2 0.3479210167730855 1105 3 0.5505227593088584 1105 4 0.42716217896478526 1105 5 0.7371133590819383 1105 6 0.5803301009152264 1105 7 0.7862596128997055 1105 8 0.7898373862218193 1105 9 0.8034397127303379 1105 10-11 0.9489291728135711 1105 12-13 0.884057711486328 1105 14-15 0.9559943355788789 1105 16-17 0.9713106066751678 1105 18-19 0.9237171812163822 1105 20-21 1.1273650288670822 1105 22-23 0.9539432428985037 1105 24-25 1.0161438648601262 1105 26-27 0.9703029453849084 1105 28-29 1.104307579885841 1105 30-31 1.0177081550015217 1105 32-33 1.0118726090939205 1105 34-35 0.914950429540184 1105 36-37 1.0324889339460412 1105 38-39 1.0350502793527028 1105 40-41 0.8914945551884728 1105 42-43 0.9025749723991936 1105 44-45 1.0156045330319756 1105 46-47 0.9753895008066849 1105 48-49 0.929351453971222 1105 50-51 0.9619659114531345 1105 52-53 0.8358112994986158 1105 54-55 0.8343136628357328 1105 56-57 0.8425846500974785 1105 58-59 0.6518376644531969 1105 60-61 0.7207929662812056 1105 62-63 0.8545539812092144 1105 64-65 0.8107819915448005 1105 66-67 0.757477269518656 1105 68-69 0.7005126262937011 1105 70-71 0.8098108360905485 1105 72-73 0.7388059791500972 1105 74-75 0.7639073404042129 1105 76-77 0.8422893292267268 1105 78-79 0.8500259227580216 1105 80-81 0.6780112823468798 1105 82-83 0.7066980965698839 1105 84-85 0.5735424085785468 1105 86-87 0.6561050424048176 1105 88-89 0.7074542713393157 1105 90-91 0.7940352875189944 1105 92-93 0.5936915301170522 1105 94-95 0.6920121264859453 1105 96-97 0.792461361312327 1105 98-99 0.7777000120040114 1105 100-101 0.7536918214137351 1105 102-103 0.4900068562689164 1105 104-105 0.42663969590936546 1105 106-107 0.7267904649321046 1105 108-109 0.8142706919249747 1105 110-111 0.7201033867240554 1105 112-113 0.517218905105679 1105 114-115 0.6523938736725547 1105 116-117 0.42031368676965997 1105 118-119 0.34530559991923226 1105 120-121 0.33464328982940117 1105 122-123 0.4396977253597001 1105 124-125 0.29845040814804946 1106 1 0.4434455113119604 1106 2 0.43723224085972134 1106 3 0.5611387862712824 1106 4 0.39960674981077915 1106 5 0.6031280455238743 1106 6 0.6361142347402691 1106 7 0.5949444575879568 1106 8 0.6502625152939316 1106 9 0.7522202820022983 1106 10-11 0.8543984306203001 1106 12-13 0.7966788168987975 1106 14-15 0.95536437710944 1106 16-17 1.0109882946764799 1106 18-19 1.019854884350913 1106 20-21 0.9818248590229715 1106 22-23 0.970148235005496 1106 24-25 0.9744674842891357 1106 26-27 0.9661215473969165 1106 28-29 1.0653243352244246 1106 30-31 1.0309220711309877 1106 32-33 0.9213164767485651 1106 34-35 0.8995228599923806 1106 36-37 0.9857421068267058 1106 38-39 1.0282805016432661 1106 40-41 1.0254356718214126 1106 42-43 1.0555650314413398 1106 44-45 1.0242470623793594 1106 46-47 1.0357446999201017 1106 48-49 1.0981172771726335 1106 50-51 1.1213801763051379 1106 52-53 1.1326017905403276 1106 54-55 1.1933356410596474 1106 56-57 1.0699189288028137 1106 58-59 0.9335158020719305 1106 60-61 1.1437757294465634 1106 62-63 1.225149479275224 1106 64-65 1.1027987578141634 1106 66-67 0.8964373598644819 1106 68-69 1.0241339165819845 1106 70-71 1.0665673902559618 1106 72-73 1.062858307903344 1106 74-75 1.053273729427783 1106 76-77 1.1394562386437102 1106 78-79 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scores pass #Quality Count 2 128382.0 3 41959.0 4 20540.0 5 16760.0 6 18100.0 7 20703.0 8 23803.0 9 24942.0 10 26909.0 11 28191.0 12 30755.0 13 32373.0 14 34109.0 15 28065.0 16 20451.0 17 18009.0 18 16724.0 19 15745.0 20 15752.0 21 16987.0 22 20356.0 23 26812.0 24 36321.0 25 47824.0 26 58330.0 27 66740.0 28 76996.0 29 89828.0 30 104874.0 31 123360.0 32 148866.0 33 185369.0 34 239044.0 35 331064.0 36 523022.0 37 693889.0 38 207609.0 39 259.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.05991791404304 19.18957403785668 13.636994783758427 25.11351326434185 2 16.338499502836655 20.604265006475632 37.304005615682755 25.75322987500496 3 18.985865023588257 24.599572675262976 29.3005661519031 27.11399614924567 4 13.467639076959381 16.956601133854374 35.37355950487984 34.2022002843064 5 14.034602620627254 37.88433384194746 33.420415939573274 14.66064759785202 6 33.17614320772356 36.239005024990114 16.369394079680703 14.215457687605623 7 28.797840564365774 32.4229967871962 20.551044870539766 18.22811777789826 8 26.75848778870842 34.54340387997146 19.0953756088791 19.602732722441022 9 26.525733497088034 15.881050487162748 19.662390766212447 37.93082524953677 10-11 25.5433942407856 26.101784386157334 26.634567654157053 21.72025371890001 12-13 26.13636890398034 23.30112689070566 27.35712151577005 23.20538268954395 14-15 23.40623410532384 24.855470381278383 25.092788738622378 26.645506774775395 16-17 23.144295532143808 27.76740975416167 25.738866897436385 23.349427816258135 18-19 22.808525604398877 26.41279694636074 27.928590731323904 22.85008671791648 20-21 23.595089413329717 25.557835088401937 27.930292650344402 22.916782847923944 22-23 23.414165921919395 25.009980690419354 28.038449468487848 23.537403919173407 24-25 23.527033093227693 25.490825103052906 27.86254481263389 23.11959699108551 26-27 23.07529702327813 25.633753597792246 28.196676687766974 23.094272691162647 28-29 22.91154129923058 25.651491409900572 28.240775743948284 23.196191546920563 30-31 23.1339127537715 25.44873387612272 28.128418798526837 23.288934571578938 32-33 23.109359961839417 25.529048283363682 28.026116851617022 23.335474903179882 34-35 23.41013118071634 25.52595326395533 27.951636345862234 23.11227920946609 36-37 23.64347688927649 25.172126534334645 27.719988308359845 23.464408268029025 38-39 23.261767020935316 25.623977828104888 27.796684778059127 23.31757037290067 40-41 23.16472890564878 25.48487332862055 28.15323745867595 23.19716030705472 42-43 23.386332166662314 25.55804546505451 27.41211972578596 23.64350264249722 44-45 23.32169979285481 25.45175011559567 27.444518293330393 23.782031798219126 46-47 23.64176304568465 25.64398675549895 27.124498253702367 23.58975194511403 48-49 23.610283585439557 25.725523476119083 26.922568036655896 23.74162490178546 50-51 23.44534059366835 25.851529819520707 26.858047322334773 23.84508226447617 52-53 23.78089273103288 25.827233302376694 26.63834218490945 23.753531781680973 54-55 23.763660093117014 25.597684378600956 26.674887115142276 23.963768413139757 56-57 23.65000351148255 25.961036589648153 26.477968958494273 23.910990940375026 58-59 23.763768425964173 25.535683611013532 26.733243993622715 23.96730396939958 60-61 23.777298415482573 25.955918582445975 26.851497069235485 23.415285932835967 62-63 23.523929005439037 25.93399332887993 27.004201333662188 23.537876332018847 64-65 23.479808713462212 25.766783185695903 26.858659238514175 23.894748862327713 66-67 23.565282697461097 25.982805598539137 26.52774865256586 23.9241630514339 68-69 23.414828456847626 25.689588527907713 27.005026119415565 23.890556895829096 70-71 23.63969322061609 25.480880785612314 26.986447636988594 23.892978356783008 72-73 23.578607602137527 25.65434524741746 26.61456054509867 24.152486605346343 74-75 23.364388707582712 26.21811390572647 26.47509047907063 23.942406907620185 76-77 23.648440238936384 25.86321442697518 26.558005335167156 23.93033999892128 78-79 23.36222380909307 25.965807864898988 26.70977530545547 23.962193020552476 80-81 23.68671808391846 26.250005267201626 26.454190094009018 23.6090865548709 82-83 23.903904916206844 25.681529127628387 26.603994738479535 23.810571217685226 84-85 23.388366999351934 25.858814249368155 26.52307517709458 24.22974357418533 86-87 23.509396595314342 25.826352604078878 26.7909573571549 23.873293443451878 88-89 23.490138480258068 25.947494839277756 26.35163027134928 24.210736409114894 90-91 23.493225223058907 25.990288278458866 26.51825006980686 23.998236428675366 92-93 23.58099813866163 25.888532366091027 26.51775772428734 24.012711770960003 94-95 23.581994831971656 25.50871198776934 26.783497931496374 24.125795248762635 96-97 23.54839955752989 25.509890611556894 26.705220093761522 24.236489737151686 98-99 23.31397073530304 25.886163317774336 26.475904155982498 24.323961790940125 100-101 23.176468357443987 25.639371847174107 26.723589300249103 24.460570495132806 102-103 23.55396440099904 25.1497899190942 26.817107836100657 24.479137843806104 104-105 23.395082823085037 25.495559916303968 26.665658179219452 24.443699081391536 106-107 23.31270574446699 25.511344705663557 26.899352573888212 24.27659697598124 108-109 23.31912665296285 25.40574161147075 26.542177715261268 24.73295402030513 110-111 22.976338843459267 25.683627314556983 26.588964288377976 24.751069553605777 112-113 23.035471763626642 25.51088360069506 26.927003906659863 24.526640729018435 114-115 22.915907609078857 25.8654801537283 26.450444928306105 24.768167308886742 116-117 22.910702634977735 25.707407139491096 26.890186904401553 24.491703321129616 118-119 22.92463514432768 25.4097818387605 26.706945008040638 24.95863800887118 120-121 22.64831983763298 25.861805695348032 26.653679252929646 24.83619521408934 122-123 22.749037369475673 25.67974061296536 26.514568187838734 25.056653829720233 124-125 22.751067843342714 25.7902040736064 26.48722029246477 24.971507790586124 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 230.0 1 195.5 2 440.5 3 877.5 4 707.5 5 601.5 6 1551.0 7 3458.0 8 5288.0 9 5776.0 10 4321.5 11 2540.0 12 2032.0 13 1995.0 14 2138.0 15 2334.5 16 2523.0 17 2812.0 18 3045.0 19 3335.5 20 3655.0 21 3969.5 22 4693.5 23 5594.0 24 6351.5 25 7798.5 26 10337.0 27 12968.5 28 14817.5 29 18080.0 30 24149.5 31 31449.0 32 41067.5 33 50024.0 34 57182.0 35 65639.0 36 77685.0 37 88311.5 38 93220.5 39 95458.5 40 94447.5 41 93762.5 42 92944.0 43 99688.5 44 111267.5 45 116553.0 46 117141.0 47 119275.5 48 131418.0 49 135267.5 50 130228.0 51 129402.5 52 132333.0 53 139269.0 54 142265.0 55 136507.0 56 126882.5 57 117841.0 58 113512.5 59 105266.5 60 87764.0 61 71033.0 62 57767.0 63 45940.5 64 36886.5 65 29781.0 66 21753.5 67 17005.0 68 12858.5 69 8638.5 70 6334.5 71 4802.5 72 3869.0 73 3174.5 74 2551.0 75 1946.5 76 1309.0 77 814.0 78 552.5 79 364.5 80 241.5 81 162.5 82 131.5 83 106.5 84 68.5 85 54.0 86 40.5 87 20.5 88 10.5 89 5.5 90 6.0 91 4.0 92 3.5 93 4.0 94 1.5 95 1.0 96 2.5 97 2.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.058654618124164636 2 0.07418910271356265 3 0.0 4 0.007921744401826834 5 0.03179934277612757 6 3.9327809087083565E-4 7 8.427387661517907E-5 8 5.618258441011939E-5 9 7.303735973315519E-4 10-11 0.06135138217585037 12-13 0.009480811119207646 14-15 0.00723350774280287 16-17 0.052643081592281864 18-19 1.1236516882023878E-4 20-21 0.010379732469769556 22-23 0.0126129902000718 24-25 0.0 26-27 0.0 28-29 0.05469374592325122 30-31 1.4045646102529847E-5 32-33 0.003595685402247641 34-35 0.0 36-37 3.792324447683058E-4 38-39 0.0 40-41 1.4045646102529847E-5 42-43 0.0033990463568122227 44-45 0.0 46-47 1.4045646102529846E-4 48-49 3.6518679866577597E-4 50-51 6.460997207163729E-4 52-53 1.1236516882023878E-4 54-55 0.0 56-57 0.002022573038764298 58-59 4.2136938307589536E-5 60-61 0.0 62-63 0.0 64-65 8.848757044593803E-4 66-67 1.4045646102529847E-5 68-69 5.056432596910745E-4 70-71 0.0 72-73 0.0027950835744034396 74-75 0.00446651546060449 76-77 0.0012921994414327458 78-79 0.0015731123634833426 80-81 0.0016012036556884025 82-83 4.0732373697336554E-4 84-85 2.8091292205059695E-5 86-87 6.180084285113132E-4 88-89 1.4045646102529847E-5 90-91 0.0 92-93 0.008048155216749602 94-95 0.006854275298034565 96-97 0.007289690327212991 98-99 0.0010674691037922683 100-101 5.47780197998664E-4 102-103 8.427387661517907E-5 104-105 9.972408732796191E-4 106-107 0.005281162934551222 108-109 0.006432905914958669 110-111 0.001193879918715037 112-113 0.003694004924965349 114-115 9.831952271770893E-4 116-117 0.0032164529574793344 118-119 0.0025984445289680214 120-121 0.013441683320121063 122-123 0.001264108149227686 124-125 0.0021630294997895963 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3559822.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.76759019754401 #Duplication Level Percentage of deduplicated Percentage of total 1 70.105318203913 22.971823375742435 2 10.683772757618346 7.001629749706454 3 5.151701854698381 5.0642656558405195 4 3.212556507848597 4.210709405425436 5 2.097312133493949 3.436193225333322 6 1.5087454301204357 2.966277117996223 7 1.1369221792390583 2.60779400390629 8 0.8639032564334168 2.2646422301707183 9 0.6792294346919254 2.0031040589485047 >10 4.071675639350931 24.286375683808725 >50 0.28294683154805184 6.418658673048829 >100 0.19047350144384445 11.659344823911274 >500 0.011278062068118771 2.4745112906365963 >1k 0.0039039445615261987 1.8827970152275713 >5k 1.7350864717894214E-4 0.43519355456756387 >10k+ 8.675432358947107E-5 0.3166801357294834 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11140 0.31293699516436496 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8958 0.2516417955729247 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6351 0.1784077967943341 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4006 0.11253371657346913 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1236516882023877E-4 2 0.0 0.0 0.0 0.0 1.6854775323035814E-4 3 0.0 0.0 0.0 0.0 1.6854775323035814E-4 4 0.0 0.0 0.0 0.0 1.9663904543541785E-4 5 0.0 0.0 0.0 0.0 1.9663904543541785E-4 6 0.0 0.0 0.0 0.0 3.090042142556566E-4 7 0.0 0.0 0.0 0.0 3.090042142556566E-4 8 0.0 0.0 0.0 0.0 3.6518679866577597E-4 9 0.0 0.0 0.0 0.0 4.213693830758954E-4 10-11 0.0 0.0 0.0 0.0 4.213693830758954E-4 12-13 0.0 0.0 0.0 0.0 4.6350632138348496E-4 14-15 0.0 0.0 0.0 0.0 8.14647473946731E-4 16-17 0.0 0.0 0.0 1.4045646102529846E-5 0.0011236516882023877 18-19 0.0 0.0 0.0 2.809129220505969E-5 0.001179834272612507 20-21 0.0 0.0 0.0 5.618258441011938E-5 0.0012079255648175667 22-23 0.0 0.0 0.0 3.370955064607163E-4 0.0014045646102529845 24-25 0.0 0.0 0.0 7.303735973315519E-4 0.0016292949478934622 26-27 0.0 0.0 0.0 0.001530975425175753 0.0016995231784061114 28-29 0.0 0.0 0.0 0.0034271376490172824 0.001741660116713701 30-31 0.0 0.0 0.0 0.00726159903500793 0.0017837970550212904 32-33 0.0 0.0 0.0 0.013301226859095763 0.0019382991621491187 34-35 0.0 0.0 0.0 0.02074541929343658 0.002191120791994656 36-37 0.0 0.0 0.0 0.03365336806166151 0.002191120791994656 38-39 0.0 0.0 0.0 0.054426878647303154 0.0023034859608148947 40-41 0.0 0.0 0.0 0.07844493348262918 0.0023456228991224843 42-43 2.809129220505969E-5 0.0 0.0 0.10086178466226682 0.002387759837430074 44-45 2.809129220505969E-5 0.0 0.0 0.12648104315328126 0.002472033714045253 46-47 2.809129220505969E-5 0.0 0.0 0.15880007483520242 0.0025984445289680214 48-49 2.809129220505969E-5 0.0 0.0 0.19283267534163226 0.0027810379283009093 50-51 2.809129220505969E-5 0.0 0.0 0.22939349214651744 0.0027810379283009093 52-53 2.809129220505969E-5 0.0 0.0 0.26588408072089 0.002991722619838857 54-55 2.809129220505969E-5 0.0 0.0 0.30469220090217997 0.0032585898957869243 56-57 2.809129220505969E-5 0.0 0.0 0.3537115058000091 0.0033569094185046332 58-59 2.809129220505969E-5 0.0 0.0 0.4084895255998755 0.0034130920029147526 60-61 2.809129220505969E-5 0.0 0.0 0.4655850770066593 0.0034973658795299316 62-63 2.809129220505969E-5 0.0 0.0 0.5235233671795949 0.0036799592788628196 64-65 2.809129220505969E-5 0.0 0.0 0.5851697079235985 0.0037080505710678792 66-67 2.809129220505969E-5 0.0 0.0 0.6597099517897242 0.0037782788015805284 68-69 2.809129220505969E-5 0.0 0.0 0.7449389323398754 0.003820415739888118 70-71 2.809129220505969E-5 0.0 0.0 0.8420786207849719 0.004101328661938715 72-73 2.809129220505969E-5 0.0 0.0 0.9411987453305249 0.004972158720295565 74-75 2.809129220505969E-5 0.0 0.0 1.0463725433462683 0.004972158720295565 76-77 2.809129220505969E-5 0.0 0.0 1.1639907838088535 0.005014295658603155 78-79 2.809129220505969E-5 0.0 0.0 1.3023403979187722 0.005070478243013274 80-81 2.809129220505969E-5 0.0 0.0 1.4576290612283422 0.005239025996243632 82-83 2.809129220505969E-5 0.0 0.0 1.6201793235729203 0.005646349733216998 84-85 2.809129220505969E-5 0.0 0.0 1.7918030732997323 0.005688486671524587 86-87 2.809129220505969E-5 0.0 0.0 1.980295643995683 0.005716577963729647 88-89 2.809129220505969E-5 0.0 0.0 2.1941406059067 0.005814897486447356 90-91 2.809129220505969E-5 0.0 0.0 2.4347284779969334 0.0058429887786524155 92-93 2.809129220505969E-5 0.0 0.0 2.6983652553414186 0.0058429887786524155 94-95 2.809129220505969E-5 0.0 0.0 2.967451743373686 0.006151992992908072 96-97 2.809129220505969E-5 0.0 0.0 3.25423574549514 0.006151992992908072 98-99 2.809129220505969E-5 0.0 0.0 3.568844172545706 0.006180084285113132 100-101 2.809129220505969E-5 1.4045646102529846E-5 0.0 3.925266488043503 0.006236266869523252 102-103 2.809129220505969E-5 2.809129220505969E-5 0.0 4.308530033243235 0.006643590606496617 104-105 2.809129220505969E-5 2.809129220505969E-5 0.0 4.698521442926079 0.006755955775316856 106-107 4.2136938307589536E-5 2.809129220505969E-5 0.0 5.101757896883608 0.006924503528547214 108-109 5.618258441011938E-5 2.809129220505969E-5 0.0 5.532453588971583 0.00727564468111046 110-111 5.618258441011938E-5 2.809129220505969E-5 0.0 6.008432444094115 0.007781287940801535 112-113 5.618258441011938E-5 2.809129220505969E-5 0.0 6.517264065450464 0.007921744401826834 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13695 0.0 30.621202 1 GTATCAA 20125 0.0 30.102093 1 TACATGG 15105 0.0 27.510723 2 ACATGGG 14470 0.0 27.343744 3 TCAACGC 23805 0.0 24.99651 4 GGTATCA 15165 0.0 24.706991 1 ATCAACG 24330 0.0 24.503633 3 CAACGCA 23935 0.0 24.442448 5 TATCAAC 24595 0.0 24.423712 2 GAGTACT 12685 0.0 24.089418 12-13 AACGCAG 24270 0.0 24.048191 6 GTACTTT 12755 0.0 24.027264 14-15 AGTACTT 13955 0.0 22.40933 12-13 ATATAGG 1150 0.0 22.260654 2 CATGGGG 9075 0.0 22.162443 4 AGAGTAC 20060 0.0 22.103518 10-11 CATGGGA 5890 0.0 21.61952 4 ACGCAGA 27040 0.0 21.386747 7 TACTTTT 14500 0.0 21.053545 14-15 CGCAGAG 27455 0.0 20.976704 8 >>END_MODULE