FastQCFastQC Report
Thu 26 May 2016
SRR1364615_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364615_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3124287
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40020.1280932257503872No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91000.043.2047581
ATCAACG106150.037.093993
CAACGCA107350.036.4597175
TCAACGC107200.036.451654
GTACATG161550.035.7679331
AACGCAG107650.035.496676
GGTATCA59000.035.237291
TATCAAC115150.034.4534232
TACATGG162750.033.893162
ACATGGG169000.030.8068143
ACGCAGA130700.028.641317
CGCAGAG127750.027.2489728
CATGGGA110200.026.9728684
CGCATAG3850.026.2750368
GCAGAGT142100.023.5748279
CATGGGG63650.023.2092694
ACGCATA4650.023.0376077
ATGGGAT46050.020.5692025
ATGGGAG50050.020.4726645
AGTACAT121350.020.2736682
Produced by FastQC (version 0.11.5)