##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364615_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3124287 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.99538742759548 31.0 27.0 31.0 10.0 34.0 2 26.46937173185434 31.0 26.0 31.0 10.0 34.0 3 27.17900756236543 31.0 27.0 31.0 10.0 34.0 4 30.577115354639314 35.0 31.0 37.0 10.0 37.0 5 30.850007057610263 35.0 32.0 37.0 10.0 37.0 6 30.90899203562285 35.0 32.0 37.0 10.0 37.0 7 30.61515283327044 35.0 31.0 37.0 10.0 37.0 8 30.441865936131986 35.0 30.0 37.0 8.0 37.0 9 31.357588787457747 37.0 30.0 39.0 8.0 39.0 10-11 31.36350309686658 37.0 30.5 39.0 8.0 39.0 12-13 31.390790762820444 37.0 30.0 39.0 8.0 39.0 14-15 32.05835859509706 38.0 30.5 40.0 8.0 41.0 16-17 32.462628113230316 38.0 31.0 40.0 7.5 41.0 18-19 32.28861737093935 38.0 30.5 40.0 7.0 41.0 20-21 32.216229975031105 38.0 30.5 40.0 7.0 41.0 22-23 32.28518266727737 38.0 30.5 40.0 2.0 41.0 24-25 32.38888568815861 38.0 31.0 40.0 2.0 41.0 26-27 32.21941838249815 38.0 30.0 40.0 2.0 41.0 28-29 32.12100584869444 38.0 30.0 40.0 2.0 41.0 30-31 31.991597762945595 38.0 30.0 40.0 2.0 41.0 32-33 31.906544437178788 38.0 30.0 40.0 2.0 41.0 34-35 31.963295145420382 38.0 30.0 40.0 2.0 41.0 36-37 31.903990894562504 38.0 30.0 40.0 2.0 41.0 38-39 31.602766647238234 38.0 30.0 40.0 2.0 41.0 40-41 31.47306505452284 37.0 30.0 40.0 2.0 41.0 42-43 30.95297743133073 37.0 28.5 40.0 2.0 41.0 44-45 30.809170700387 37.0 29.0 40.0 2.0 41.0 46-47 30.770195247747726 37.0 28.5 40.0 2.0 41.0 48-49 30.69464601043374 37.0 29.0 40.0 2.0 41.0 50-51 30.45866672940098 36.0 28.0 40.0 2.0 41.0 52-53 30.35432836355943 36.0 28.0 40.0 2.0 41.0 54-55 30.1757527397451 36.0 28.0 40.0 2.0 41.0 56-57 29.940979173808294 36.0 28.0 40.0 2.0 41.0 58-59 29.71727357313845 35.0 27.5 39.0 2.0 41.0 60-61 29.40902148234141 35.0 27.0 39.0 2.0 40.0 62-63 29.216792343341055 35.0 26.5 39.0 2.0 40.0 64-65 28.983987706635148 34.5 26.0 39.0 2.0 40.0 66-67 28.712086949758458 34.0 26.0 38.0 2.0 40.0 68-69 28.465392423935445 34.0 26.0 38.0 2.0 40.0 70-71 28.037614982234345 34.0 26.0 37.0 2.0 40.0 72-73 27.80156384480683 34.0 26.0 37.0 2.0 39.0 74-75 27.773580980236453 34.0 26.0 36.0 2.0 39.0 76-77 27.52077161925265 34.0 26.0 36.0 2.0 39.0 78-79 27.22618536645321 33.0 25.5 35.5 2.0 38.0 80-81 26.81818571725325 33.0 25.0 35.0 2.0 37.0 82-83 26.937758599001945 33.0 25.5 35.0 2.0 37.0 84-85 26.77083859453373 33.0 25.0 35.0 2.0 36.5 86-87 26.636735997685232 33.0 25.0 35.0 2.0 36.0 88-89 26.471912311512995 33.0 25.0 35.0 2.0 36.0 90-91 26.337487081052412 33.0 25.0 35.0 2.0 35.5 92-93 26.20417042352383 33.0 24.0 35.0 2.0 35.0 94-95 26.096664775035073 33.0 24.0 35.0 2.0 35.0 96-97 25.987241729072906 33.0 24.0 35.0 2.0 35.0 98-99 25.89696433138185 33.0 23.5 35.0 2.0 35.0 100-101 25.791308544957616 33.0 23.0 35.0 2.0 35.0 102-103 24.61910269447077 31.0 19.5 34.0 2.0 35.0 104-105 25.48261347309002 32.5 21.5 35.0 2.0 35.0 106-107 25.645269624717578 33.0 23.0 35.0 2.0 35.0 108-109 25.60205032380188 33.0 22.0 35.0 2.0 35.0 110-111 25.530954422561052 33.0 20.0 35.0 2.0 35.0 112-113 25.42947718951556 33.0 19.5 35.0 2.0 35.0 114-115 25.321211047512598 33.0 19.0 35.0 2.0 35.0 116-117 25.20657433199959 33.0 18.5 35.0 2.0 35.0 118-119 25.072105571607217 33.0 17.0 35.0 2.0 35.0 120-121 24.87617478803964 33.0 6.0 35.0 2.0 35.0 122-123 24.66104298356713 33.0 2.0 35.0 2.0 35.0 124-125 23.683504908479918 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7255964391651055 1101 2 2.1527786160672697 1101 3 1.6614180496188524 1101 4 1.6738075759902848 1101 5 1.5471226680914114 1101 6 1.5034257475476451 1101 7 1.4849469745016854 1101 8 1.5720230181846873 1101 9 2.032878589687126 1101 10-11 2.0019512919031364 1101 12-13 2.0636561975818886 1101 14-15 2.443715875297613 1101 16-17 1.9790452485936854 1101 18-19 1.9974992693758153 1101 20-21 2.1917463063924245 1101 22-23 2.0504693967002154 1101 24-25 1.9612741349785594 1101 26-27 2.265115587050488 1101 28-29 2.2726330230208234 1101 30-31 2.3633559530312525 1101 32-33 2.2967683759620563 1101 34-35 2.174484419081857 1101 36-37 1.9995718910856795 1101 38-39 2.0581847268282942 1101 40-41 2.182696311140983 1101 42-43 2.2967032238398133 1101 44-45 2.018209973503044 1101 46-47 1.866741633354895 1101 48-49 1.712946134131382 1101 50-51 1.765891077942797 1101 52-53 1.6316130023081286 1101 54-55 1.712434232137376 1101 56-57 1.6741245701934098 1101 58-59 1.6722422555921383 1101 60-61 1.677416176363657 1101 62-63 1.4975835011912828 1101 64-65 1.536854078473329 1101 66-67 1.4983572676428523 1101 68-69 1.3027576250343316 1101 70-71 1.2677688757994652 1101 72-73 1.131334302192542 1101 74-75 1.1862303790664797 1101 76-77 1.3246845065086568 1101 78-79 1.347635690968378 1101 80-81 1.5497375591145364 1101 82-83 1.4628340520534877 1101 84-85 1.4173921535345073 1101 86-87 1.4090290475573077 1101 88-89 1.4335944344384153 1101 90-91 1.40130987619904 1101 92-93 1.4938038935163576 1101 94-95 1.4380496670601524 1101 96-97 1.3212026283559588 1101 98-99 1.3090981868502034 1101 100-101 1.2854282249844857 1101 102-103 1.1899470383398238 1101 104-105 1.372410929452876 1101 106-107 1.444673433158048 1101 108-109 1.3817040579221924 1101 110-111 1.4787139069614952 1101 112-113 1.4066205322745944 1101 114-115 1.3514249673293577 1101 116-117 1.4379405058972488 1101 118-119 1.316790756050942 1101 120-121 1.329618961964492 1101 122-123 1.4021357236151317 1101 124-125 1.2310151065216246 1102 1 1.2313162983298547 1102 2 1.6920865970591201 1102 3 1.3573472447540063 1102 4 1.2819937329097328 1102 5 1.1210075807481488 1102 6 0.9578652699613563 1102 7 0.9603077401475524 1102 8 1.0701144696157172 1102 9 1.6968084362215983 1102 10-11 1.723430941047635 1102 12-13 1.7798328482228918 1102 14-15 2.0019495691202778 1102 16-17 1.7216093352371473 1102 18-19 1.490946033325585 1102 20-21 1.8823844205454705 1102 22-23 1.8024959264346379 1102 24-25 1.4777802465315162 1102 26-27 1.961999791269136 1102 28-29 1.6280123855110418 1102 30-31 1.7006698686272799 1102 32-33 1.9182484016910486 1102 34-35 1.7973479565273536 1102 36-37 1.6783486500544313 1102 38-39 1.8768049653422167 1102 40-41 1.9727212263382903 1102 42-43 2.172684146069514 1102 44-45 1.7719074361777878 1102 46-47 1.6658758288707638 1102 48-49 1.5174661767387043 1102 50-51 1.6371657503119863 1102 52-53 1.5967879611662745 1102 54-55 1.7118666687924247 1102 56-57 1.7648078377453942 1102 58-59 1.6647911640436206 1102 60-61 1.6206435844400744 1102 62-63 1.3470854819797786 1102 64-65 1.135484863189987 1102 66-67 1.2783195467358652 1102 68-69 1.2145009163882925 1102 70-71 1.3835279172027235 1102 72-73 1.019339934610624 1102 74-75 1.1985488481200974 1102 76-77 0.9579321546237018 1102 78-79 1.0124309011075745 1102 80-81 1.0603549914310513 1102 82-83 1.1143275172562994 1102 84-85 1.0789567279926544 1102 86-87 1.1761770290047338 1102 88-89 1.094861369374076 1102 90-91 1.1576406748125834 1102 92-93 1.219750248045301 1102 94-95 1.129314449953224 1102 96-97 0.9596299435537787 1102 98-99 1.1036116906648665 1102 100-101 1.033920210761348 1102 102-103 0.9496873732299811 1102 104-105 1.0221575018681008 1102 106-107 0.9881429253595826 1102 108-109 1.1007672746095523 1102 110-111 0.9786390263502582 1102 112-113 0.8532267298817011 1102 114-115 0.9001477902140458 1102 116-117 1.1297990293498792 1102 118-119 1.1091514135895046 1102 120-121 1.1015616217403377 1102 122-123 0.9146102456077116 1102 124-125 0.8642726284983162 1103 1 1.3486447758379008 1103 2 1.758844054642438 1103 3 1.3851185351617374 1103 4 1.2552365463440793 1103 5 1.272429711359031 1103 6 1.284242575495469 1103 7 1.1965758675072315 1103 8 1.5066731297523326 1103 9 2.0122136285973546 1103 10-11 2.0079952453575096 1103 12-13 1.939585153751235 1103 14-15 2.1514188054584196 1103 16-17 1.772246542468352 1103 18-19 1.7524780076712787 1103 20-21 2.038990201803891 1103 22-23 1.911431775125287 1103 24-25 1.6743714573642237 1103 26-27 2.013142114580944 1103 28-29 2.006576146771579 1103 30-31 2.068153270984844 1103 32-33 2.158123115019581 1103 34-35 1.882137998269151 1103 36-37 1.728992217904235 1103 38-39 1.8106213461751324 1103 40-41 1.88798709351207 1103 42-43 2.096268550072409 1103 44-45 1.8226447770603436 1103 46-47 1.7468477710298806 1103 48-49 1.595528158938837 1103 50-51 1.5560141898148743 1103 52-53 1.4464319485864436 1103 54-55 1.4691982611599173 1103 56-57 1.563012258163294 1103 58-59 1.476723804355295 1103 60-61 1.5486886257317778 1103 62-63 1.3560010642646816 1103 64-65 1.2381633611603284 1103 66-67 1.2538624842990629 1103 68-69 1.2590566808763413 1103 70-71 1.3819750044381003 1103 72-73 1.2796200424258757 1103 74-75 1.3353403575976728 1103 76-77 1.259181421596974 1103 78-79 1.1888007836154273 1103 80-81 1.2429143089237726 1103 82-83 1.2625371667479612 1103 84-85 1.2746705882829588 1103 86-87 1.2778307760105783 1103 88-89 1.3459346431010424 1103 90-91 1.363781225217192 1103 92-93 1.3183642415997845 1103 94-95 1.3382340375841792 1103 96-97 1.3808306135573751 1103 98-99 1.2906335812868406 1103 100-101 1.330037426453103 1103 102-103 1.1226738214711638 1103 104-105 1.289519421019751 1103 106-107 1.3302034422206823 1103 108-109 1.4063180712240246 1103 110-111 1.3916710027931067 1103 112-113 1.367039873251933 1103 114-115 1.3311040128878737 1103 116-117 1.3695104868012784 1103 118-119 1.4252004111859762 1103 120-121 1.4776485212489732 1103 122-123 1.321487056050632 1103 124-125 1.0572523907153482 1104 1 1.3947088255121365 1104 2 1.8110282882648683 1104 3 1.2638775339204464 1104 4 1.4236546878255076 1104 5 0.9420957962836845 1104 6 1.0378814866582644 1104 7 0.8017052758760101 1104 8 1.1444115361339975 1104 9 1.574576331637612 1104 10-11 1.6032682992531058 1104 12-13 1.591669366450315 1104 14-15 1.704300686029157 1104 16-17 1.4533567787491393 1104 18-19 1.4579099380105944 1104 20-21 1.7971064703824453 1104 22-23 1.6634536612030288 1104 24-25 1.6921915898304647 1104 26-27 1.774149757856577 1104 28-29 1.8269251032844522 1104 30-31 1.8851234473337115 1104 32-33 1.881079872196917 1104 34-35 1.7717968991033004 1104 36-37 1.6617306666163998 1104 38-39 1.835170012341088 1104 40-41 1.8229910302485344 1104 42-43 2.050834216973307 1104 44-45 1.7882383454045474 1104 46-47 1.660668114745473 1104 48-49 1.4507052395131304 1104 50-51 1.4495302054586148 1104 52-53 1.3416315086717674 1104 54-55 1.4193153956995488 1104 56-57 1.4798229493155333 1104 58-59 1.4633913095690048 1104 60-61 1.436891962100816 1104 62-63 1.460337279700319 1104 64-65 1.234703548325637 1104 66-67 1.3474124852867746 1104 68-69 1.1568942373825202 1104 70-71 1.2705341185776575 1104 72-73 1.1802161364898893 1104 74-75 1.0862610459641644 1104 76-77 1.1672462283824352 1104 78-79 1.227162093377526 1104 80-81 1.3486204199562728 1104 82-83 1.2920415249752537 1104 84-85 1.2772483141353206 1104 86-87 1.2555439695403798 1104 88-89 1.223418889100106 1104 90-91 1.1731750484313181 1104 92-93 1.20237363332663 1104 94-95 1.1516349465207902 1104 96-97 1.175833276347376 1104 98-99 1.1883923224342219 1104 100-101 1.2589818529574828 1104 102-103 1.1484694232113064 1104 104-105 1.2057542158035055 1104 106-107 1.2571786941980214 1104 108-109 1.2374643287442382 1104 110-111 1.1534353541427045 1104 112-113 1.1641066518323768 1104 114-115 1.2362387428051775 1104 116-117 1.207523759227069 1104 118-119 1.2314922150740095 1104 120-121 1.3060563985921156 1104 122-123 1.296941413491787 1104 124-125 0.9396098850884158 1105 1 1.1375871234678847 1105 2 1.5360481427566448 1105 3 1.0081852257631922 1105 4 1.086056529786994 1105 5 0.8792623039633121 1105 6 0.8313168436926475 1105 7 0.7104154128164168 1105 8 0.8773936084831391 1105 9 1.1263165003991737 1105 10-11 1.0616686543133689 1105 12-13 1.108161255304875 1105 14-15 1.4820675192814292 1105 16-17 0.9161165520269279 1105 18-19 1.0155555741346944 1105 20-21 0.9870001185209176 1105 22-23 1.1260892238304336 1105 24-25 0.7954749474813667 1105 26-27 1.149192418839867 1105 28-29 1.2585343608419493 1105 30-31 1.1961672075186414 1105 32-33 1.2916491321548698 1105 34-35 1.0527706941963118 1105 36-37 0.7154348488282807 1105 38-39 1.0712991214761374 1105 40-41 1.0565763898841318 1105 42-43 1.2902075036531713 1105 44-45 1.0826356765809244 1105 46-47 0.5676967019756027 1105 48-49 0.6470745280952279 1105 50-51 0.8210742448692692 1105 52-53 0.5951813450111736 1105 54-55 0.7270986586921815 1105 56-57 0.6975922607365028 1105 58-59 0.7819088638755431 1105 60-61 0.6398544098579748 1105 62-63 0.6973710283427721 1105 64-65 0.4986660968933201 1105 66-67 0.27389478311041415 1105 68-69 0.25279429292544364 1105 70-71 0.5153416535920421 1105 72-73 0.3560899769800123 1105 74-75 0.4388560534040664 1105 76-77 0.514520555828387 1105 78-79 0.49149669105180394 1105 80-81 0.5987295306153548 1105 82-83 0.6113745651593625 1105 84-85 0.6013919805686356 1105 86-87 0.6493652128427918 1105 88-89 0.6709421775177091 1105 90-91 0.5323784121783426 1105 92-93 0.5721934458535998 1105 94-95 0.4357842946091601 1105 96-97 0.42791680531140486 1105 98-99 0.5445229512824845 1105 100-101 0.508317593654688 1105 102-103 0.44699454586231013 1105 104-105 0.5804013412033768 1105 106-107 0.5689551115734375 1105 108-109 0.5807367375522716 1105 110-111 0.5897461812574107 1105 112-113 0.5968073397832327 1105 114-115 0.6964259697452029 1105 116-117 0.6549777970769952 1105 118-119 0.6129043308580258 1105 120-121 0.6449797508635804 1105 122-123 0.5041875312613584 1105 124-125 0.35484034722154334 1106 1 0.32435745875245203 1106 2 -2.2168019994898067 1106 3 -0.2763181032506168 1106 4 0.2881939502853754 1106 5 0.560207237457508 1106 6 0.5113174394893036 1106 7 0.5416068571764576 1106 8 0.5558347065363733 1106 9 1.0482403277968864 1106 10-11 1.1920356650526038 1106 12-13 1.0858017516034941 1106 14-15 1.2850085205177137 1106 16-17 0.9631971484193826 1106 18-19 0.9664090528424083 1106 20-21 1.1784519260190152 1106 22-23 0.8773538022298268 1106 24-25 1.0207315124157574 1106 26-27 0.9324088270797333 1106 28-29 1.1083965828655735 1106 30-31 1.0842847879884374 1106 32-33 1.191097275503541 1106 34-35 1.0244629056445476 1106 36-37 0.7519743129091978 1106 38-39 1.0846524139973965 1106 40-41 1.0848342804660795 1106 42-43 1.2967024319341434 1106 44-45 1.2148504016952408 1106 46-47 1.0022380130261475 1106 48-49 0.9076305553231343 1106 50-51 1.0424953688396599 1106 52-53 0.9047274969095298 1106 54-55 0.876654790184503 1106 56-57 0.8220139656086296 1106 58-59 0.8019276314701642 1106 60-61 0.9185479767435965 1106 62-63 0.8197260635692523 1106 64-65 0.7638000766660227 1106 66-67 0.8176858372236424 1106 68-69 0.7139170717210419 1106 70-71 0.8525081936519214 1106 72-73 0.6767834842859699 1106 74-75 0.7074225666530936 1106 76-77 0.5946924431632432 1106 78-79 0.6023833854230141 1106 80-81 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2216 100-101 -3.1171427682975335 2216 102-103 -2.8244326811569245 2216 104-105 -2.8736749389276817 2216 106-107 -2.8590248596231085 2216 108-109 -3.004628539498434 2216 110-111 -2.833221045972792 2216 112-113 -3.1378298155127986 2216 114-115 -2.9810843165374585 2216 116-117 -2.8497860036467912 2216 118-119 -2.910963030130567 2216 120-121 -3.0311896835971908 2216 122-123 -2.766853167732215 2216 124-125 -2.660975263521898 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 248125.0 3 34063.0 4 20205.0 5 16564.0 6 16319.0 7 18378.0 8 20357.0 9 21932.0 10 23706.0 11 25885.0 12 26728.0 13 26799.0 14 26887.0 15 23259.0 16 19317.0 17 17006.0 18 15177.0 19 14356.0 20 14268.0 21 15032.0 22 18803.0 23 27002.0 24 39864.0 25 51807.0 26 57611.0 27 62051.0 28 71601.0 29 81726.0 30 95507.0 31 109814.0 32 133217.0 33 168416.0 34 220331.0 35 302174.0 36 437183.0 37 485924.0 38 116800.0 39 93.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.16593704398583 19.064768044130144 13.88101537820112 22.888279533682912 2 13.456259608795303 20.776012083661648 41.39019857963076 24.377529727912282 3 18.948992604828064 26.20999283882608 28.46722310475247 26.373791451593387 4 11.703867932430814 17.12007096735059 37.27172388206043 33.90433721815816 5 13.079925081272181 40.15869825821021 32.904847180049586 13.85652948046803 6 31.413951313054 37.46172725135183 17.563586893935287 13.560734541658876 7 28.744623457601893 33.5398738176418 20.129133242027 17.58636948272931 8 25.275895962376875 37.468777007948255 19.334572258237273 17.920754771437597 9 26.00192603771455 17.91542173844305 19.751410936469295 36.3312412873731 10-11 23.828414709270312 28.800504864351357 27.073110460550904 20.297969965827427 12-13 24.76522815178381 25.88337786782633 27.42936327162621 21.92203070876365 14-15 21.945986519234555 26.908289497225546 25.442523985078942 25.703199998460953 16-17 22.050272712556318 30.337144203475784 25.573674062415353 22.03890902155255 18-19 22.066635405018697 28.82399291402646 27.600716613161154 21.50865506779369 20-21 23.108590137927685 27.70004515885674 27.599403588886723 21.591961114328857 22-23 22.87528220323764 27.625869057697734 27.17511352266605 22.323735216398575 24-25 22.48546267640669 27.636426543167218 27.42532291223928 22.45278786818681 26-27 22.334149165416292 27.869632407173032 27.451370087803806 22.34484833960687 28-29 22.250717484303827 27.841548817144734 27.626127200541674 22.28160649800977 30-31 22.35462679678122 27.523603162367 27.64904329263113 22.472726748220648 32-33 22.270992161412913 27.50386750766086 27.544959526045332 22.68018080488089 34-35 22.63802961273436 27.439409412046768 27.54558357164424 22.37697740357463 36-37 22.687237448323035 27.36500845229693 27.27010040828729 22.677653691092747 38-39 22.384974499696927 27.525097514832623 27.515398534315715 22.57452945115473 40-41 22.537028829668987 27.380745457542478 27.58379229861916 22.498433414169376 42-43 22.306279981009187 27.32223909973659 27.625922043640045 22.74555887561418 44-45 22.528338548456567 27.507655178969216 27.301069937792004 22.66293633478222 46-47 22.545226088371077 27.78504956739218 26.891578391485837 22.778145952750908 48-49 22.601209876986434 27.578409286152063 26.956113109906987 22.86426772695451 50-51 22.627285160786105 27.660573331989102 26.958874679175505 22.75326682804929 52-53 22.839132204515835 27.674379346227646 26.809065531297648 22.67742291795887 54-55 22.597963626104825 27.640313278427325 26.930147117755116 22.831575977712735 56-57 22.579981245942633 27.864073156852843 26.847426927299978 22.708518669904546 58-59 22.737356334456766 27.366464100500938 26.89502942340196 23.001150141640338 60-61 22.753402987801653 27.442642446272618 27.13599289671874 22.66796166920699 62-63 22.565875737939994 27.62740933261923 27.051945142360438 22.754769787080335 64-65 22.4896587122655 27.504067527771603 26.94281039126949 23.06346336869341 66-67 22.489900637527263 27.781402559979284 26.748056557711696 22.980640244781757 68-69 22.477714935443778 27.582188869156855 26.976661311083333 22.963434884316037 70-71 22.370953661638175 27.62987957768867 27.05875811446914 22.94040864620402 72-73 22.613342825962555 27.553349580169034 26.726311735465803 23.10699585840261 74-75 22.435799874590796 27.75298679935136 26.699168178743683 23.112045147314163 76-77 22.622196285118843 27.504375283820597 26.86994711202425 23.003481319036307 78-79 22.618805721981246 27.698740264177236 26.77619674262173 22.906257271219783 80-81 22.39521347708843 27.846946144405344 26.933454664225092 22.824385714281135 82-83 22.601814686572713 27.91136354712488 26.683377019045153 22.803444747257256 84-85 22.62941526564747 27.62928866994063 26.711485820159098 23.029810244252797 86-87 22.476777029671737 27.498506940203555 27.06733884010331 22.95737719002139 88-89 22.61930578121371 27.599401649934443 26.885170544674548 22.896122024177306 90-91 22.508201266368477 27.786130845255286 26.920266222641747 22.785401665734494 92-93 22.551450380113486 27.53767840781994 26.91758307535425 22.993288136712327 94-95 22.44095813435783 27.513663269336313 26.96945672635504 23.07592186995081 96-97 22.530155433106707 27.51621165059125 26.912894633086193 23.040738283215852 98-99 22.362334098390978 27.46908984958532 26.817719741452077 23.350856310571626 100-101 22.169666503439313 27.642312247948336 27.02399249837841 23.164028750233946 102-103 22.38309056145512 27.375521439114248 27.006939484209674 23.234448515220958 104-105 22.31280162757716 27.31628427993058 27.235078473042414 23.13583561944985 106-107 22.169590385578367 27.577974962443662 27.080108162243366 23.1723264897346 108-109 22.18458836762929 27.623286664682677 26.72020236062827 23.47192260705977 110-111 22.06848054818277 27.67768306645505 27.01786548001401 23.23597090534817 112-113 22.16487867257319 27.635815377694428 27.09617295087995 23.103132998852434 114-115 22.00456518005675 27.655657644818227 26.859010014687524 23.480767160437498 116-117 21.74869985483361 27.596897883512185 27.116091296056606 23.5383109655976 118-119 21.894952808072276 27.770808747947 26.822757272135018 23.511481171845702 120-121 21.8921098180623 27.71504211191637 27.0185320623034 23.37431600771793 122-123 21.90884755610271 27.66941682148838 26.93461984353982 23.487115778869093 124-125 21.870574643660433 27.5224861572811 26.99675041963533 23.61018877942314 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5609.0 1 4103.5 2 2145.0 3 1423.0 4 1073.0 5 954.5 6 929.5 7 1014.5 8 1238.0 9 1372.0 10 1372.0 11 1410.5 12 1543.5 13 1716.0 14 1859.0 15 2060.0 16 2194.5 17 2354.0 18 2689.0 19 3080.0 20 3518.5 21 4287.5 22 5480.5 23 7180.0 24 9326.0 25 12108.5 26 16317.5 27 21039.5 28 25095.5 29 30026.0 30 36633.5 31 43069.0 32 48936.5 33 55173.0 34 61738.5 35 67739.0 36 74698.5 37 83044.5 38 88083.0 39 90204.5 40 93327.0 41 97655.0 42 101803.0 43 106667.5 44 112809.5 45 116870.0 46 116704.5 47 116249.0 48 118837.0 49 120033.5 50 118779.5 51 115253.5 52 110878.0 53 107449.0 54 103102.5 55 95352.0 56 85770.5 57 78036.5 58 72111.5 59 65896.0 60 56685.5 61 46021.0 62 35764.0 63 26714.0 64 20364.0 65 15979.5 66 12067.5 67 9091.5 68 6783.0 69 4877.5 70 3644.5 71 2740.5 72 2171.0 73 1797.5 74 1369.0 75 1050.0 76 724.5 77 462.5 78 346.0 79 265.5 80 183.0 81 143.5 82 102.0 83 67.0 84 55.5 85 46.5 86 31.0 87 23.5 88 19.5 89 14.0 90 11.5 91 10.0 92 9.0 93 6.0 94 5.5 95 7.0 96 4.5 97 1.5 98 1.0 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1996295474775525 2 0.2352856827813834 3 0.28409681953034405 4 0.271677986049297 5 0.30230897481569396 6 0.21957009711335737 7 0.21051203042486175 8 0.19178775829493255 9 0.19101958302806368 10-11 0.1357429711162899 12-13 0.15946038248086683 14-15 0.17517596814889286 16-17 0.1504983377007298 18-19 0.17442379653341708 20-21 0.1695586865099141 22-23 0.19098757572527747 24-25 0.18168945426588531 26-27 0.23122075532753553 28-29 0.1617969155842597 30-31 0.14313665805990294 32-33 0.18168945426588531 34-35 0.14124822719551694 36-37 0.14163231482895136 38-39 0.1729194533024655 40-41 0.19335611613145656 42-43 0.25745073996082946 44-45 0.1598284664629082 46-47 0.1454571875119027 48-49 0.14822581920290934 50-51 0.1531709474833778 52-53 0.17343157014704474 54-55 0.16058063807838396 56-57 0.15882023642514276 58-59 0.135598938253752 60-61 0.14729760742210943 62-63 0.13711928513609667 64-65 0.13278229560856605 66-67 0.12140369946807064 68-69 0.12378824352564281 70-71 0.12594873646371155 72-73 0.1046318728080999 74-75 0.10575212840561704 76-77 0.1254046123163461 78-79 0.131518007148511 80-81 0.14224045358188925 82-83 0.1428966032890064 84-85 0.13180607287358684 86-87 0.15536344772423277 88-89 0.1507063851688401 90-91 0.13897570869769646 92-93 0.13966386570759984 94-95 0.1592363313613634 96-97 0.1477777169639025 98-99 0.14334470552801326 100-101 0.1246684443522634 102-103 0.16115676952853564 104-105 0.12228390029469123 106-107 0.1272130249237666 108-109 0.13803149326550346 110-111 0.11445811476346443 112-113 0.10663232923223763 114-115 0.1046958874136723 116-117 0.10836072358269262 118-119 0.09402145193447337 120-121 0.08718789278961887 122-123 0.11180150863220953 124-125 0.10954499378578217 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3124287.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.72287014415622 #Duplication Level Percentage of deduplicated Percentage of total 1 75.61806716424789 39.11183468488477 2 11.672465543766243 12.074668391647185 3 4.798776274885317 7.446194463502531 4 2.399675582516191 4.964724341703495 5 1.4383407433450899 3.7197555745543607 6 0.9288180456116251 2.8824681096431433 7 0.6053189252979364 2.1916182518289755 8 0.4346722728392042 1.798599801866192 9 0.3379234573027946 1.5730533990663091 >10 1.6025535980326633 14.658402497098951 >50 0.10763274460384241 3.8387370186761194 >100 0.05406862813913434 4.874449886356445 >500 0.001427477964120168 0.5295186976170224 >1k 2.5954144797641677E-4 0.3359748815545566 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4002 0.1280932257503872 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.20073027862037E-5 0.0 0.0 0.0 6 0.0 3.20073027862037E-5 0.0 0.0 0.0 7 0.0 3.20073027862037E-5 0.0 0.0 0.0 8 0.0 3.20073027862037E-5 0.0 0.0 0.0 9 0.0 3.20073027862037E-5 0.0 0.0 0.0 10-11 0.0 3.20073027862037E-5 0.0 0.0 0.0 12-13 0.0 3.20073027862037E-5 0.0 3.20073027862037E-5 0.0 14-15 0.0 3.20073027862037E-5 0.0 8.001825696550926E-5 0.0 16-17 0.0 3.20073027862037E-5 0.0 9.60219083586111E-5 0.0 18-19 0.0 3.20073027862037E-5 0.0 9.60219083586111E-5 0.0 20-21 0.0 3.20073027862037E-5 0.0 1.4403286253791665E-4 0.0 22-23 0.0 3.20073027862037E-5 0.0 3.0406937646893515E-4 0.0 24-25 0.0 3.20073027862037E-5 0.0 4.160949362206481E-4 0.0 26-27 0.0 3.20073027862037E-5 0.0 5.441241473654629E-4 0.0 28-29 0.0 6.40146055724074E-5 0.0 0.0011362592489102313 0.0 30-31 0.0 6.40146055724074E-5 0.0 0.0027046170854342125 0.0 32-33 0.0 6.40146055724074E-5 0.0 0.006625511676744166 0.0 34-35 0.0 6.40146055724074E-5 0.0 0.011730676471143656 0.0 36-37 0.0 6.40146055724074E-5 0.0 0.020628706645708286 0.0 38-39 0.0 6.40146055724074E-5 0.0 0.03503199289949995 0.0 40-41 0.0 6.40146055724074E-5 0.0 0.05241195831240856 0.0 42-43 1.600365139310185E-5 6.40146055724074E-5 0.0 0.06937582878909652 0.0 44-45 3.20073027862037E-5 6.40146055724074E-5 0.0 0.0899565244806255 0.0 46-47 3.20073027862037E-5 6.40146055724074E-5 0.0 0.11429807824953342 0.0 48-49 3.20073027862037E-5 6.40146055724074E-5 0.0 0.14312065440850985 0.0 50-51 3.20073027862037E-5 6.40146055724074E-5 0.0 0.17536801196561008 0.0 52-53 3.20073027862037E-5 6.40146055724074E-5 0.0 0.20567892770414498 0.0 54-55 3.20073027862037E-5 6.40146055724074E-5 0.0 0.23965467961170023 0.0 56-57 3.20073027862037E-5 6.40146055724074E-5 0.0 0.2800959066820686 0.0 58-59 3.20073027862037E-5 6.40146055724074E-5 0.0 0.3280108389530155 0.0 60-61 3.20073027862037E-5 6.40146055724074E-5 0.0 0.3822312098728446 0.0 62-63 3.20073027862037E-5 6.40146055724074E-5 0.0 0.4378118911610873 0.0 64-65 4.801095417930555E-5 6.40146055724074E-5 0.0 0.49152014523633714 3.20073027862037E-5 66-67 6.40146055724074E-5 6.40146055724074E-5 0.0 0.5562229078186479 3.20073027862037E-5 68-69 9.60219083586111E-5 6.40146055724074E-5 0.0 0.6327203614776747 3.20073027862037E-5 70-71 9.60219083586111E-5 6.40146055724074E-5 0.0 0.7200522871298316 3.20073027862037E-5 72-73 1.1202555975171296E-4 6.40146055724074E-5 0.0 0.81188123882345 3.20073027862037E-5 74-75 1.280292111448148E-4 6.40146055724074E-5 0.0 0.9079511581362403 3.20073027862037E-5 76-77 1.4403286253791665E-4 6.40146055724074E-5 0.0 1.0116868264663266 3.20073027862037E-5 78-79 1.600365139310185E-4 6.40146055724074E-5 0.0 1.136499303681128 3.20073027862037E-5 80-81 1.920438167172222E-4 6.40146055724074E-5 0.0 1.2769953592611691 3.20073027862037E-5 82-83 1.920438167172222E-4 6.40146055724074E-5 0.0 1.4320387339575398 3.20073027862037E-5 84-85 1.920438167172222E-4 6.40146055724074E-5 0.0 1.5861538968731106 3.20073027862037E-5 86-87 1.920438167172222E-4 6.40146055724074E-5 0.0 1.7496792068078253 3.20073027862037E-5 88-89 1.920438167172222E-4 6.40146055724074E-5 0.0 1.9388743735770753 3.20073027862037E-5 90-91 2.240511195034259E-4 6.40146055724074E-5 0.0 2.158972591186405 6.40146055724074E-5 92-93 2.240511195034259E-4 6.40146055724074E-5 0.0 2.3955545697306295 6.40146055724074E-5 94-95 2.240511195034259E-4 6.40146055724074E-5 0.0 2.63959424982404 6.40146055724074E-5 96-97 2.240511195034259E-4 6.40146055724074E-5 0.0 2.8853143133137253 6.40146055724074E-5 98-99 2.560584222896296E-4 6.40146055724074E-5 0.0 3.1542236676720163 6.40146055724074E-5 100-101 2.560584222896296E-4 6.40146055724074E-5 0.0 3.4740246334603704 6.40146055724074E-5 102-103 2.880657250758333E-4 6.40146055724074E-5 0.0 3.8263770261822936 8.001825696550926E-5 104-105 2.880657250758333E-4 6.40146055724074E-5 0.0 4.181081955659003 9.60219083586111E-5 106-107 2.880657250758333E-4 6.40146055724074E-5 0.0 4.5315139102137545 9.60219083586111E-5 108-109 2.880657250758333E-4 6.40146055724074E-5 0.0 4.904927108169 9.60219083586111E-5 110-111 2.880657250758333E-4 6.40146055724074E-5 0.0 5.329760038050281 9.60219083586111E-5 112-113 2.880657250758333E-4 6.40146055724074E-5 0.0 5.7925056180818215 9.60219083586111E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9100 0.0 43.204758 1 ATCAACG 10615 0.0 37.09399 3 CAACGCA 10735 0.0 36.459717 5 TCAACGC 10720 0.0 36.45165 4 GTACATG 16155 0.0 35.767933 1 AACGCAG 10765 0.0 35.49667 6 GGTATCA 5900 0.0 35.23729 1 TATCAAC 11515 0.0 34.453423 2 TACATGG 16275 0.0 33.89316 2 ACATGGG 16900 0.0 30.806814 3 ACGCAGA 13070 0.0 28.64131 7 CGCAGAG 12775 0.0 27.248972 8 CATGGGA 11020 0.0 26.972868 4 CGCATAG 385 0.0 26.275036 8 GCAGAGT 14210 0.0 23.574827 9 CATGGGG 6365 0.0 23.209269 4 ACGCATA 465 0.0 23.037607 7 ATGGGAT 4605 0.0 20.569202 5 ATGGGAG 5005 0.0 20.472664 5 AGTACAT 12135 0.0 20.273668 2 >>END_MODULE