FastQCFastQC Report
Thu 26 May 2016
SRR1364615_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364615_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3124287
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111590.35716949179124713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94340.3019568944850457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61370.1964288171989321No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82100.054.709561
GTATCAA137400.047.474721
ATCAACG170700.037.8904843
TATCAAC173550.037.257832
TCAACGC174450.037.0095444
CAACGCA176600.036.088225
AACGCAG181450.035.1440476
GTACATG161600.032.734571
TACATGG163450.031.436792
ACATGGG167000.029.5017643
ACGCAGA221150.028.5937297
CGCAGAG220900.028.464368
GCAGAGT236850.025.4162229
CATGGGA110350.025.3982374
GAGTACT137900.024.2047312-13
GTACTTT144700.023.17014514-15
AGTACTT143300.022.64848712-13
CATGGGG62200.022.2905394
AGTACAT124450.022.1991042
CAGAGTA234450.021.90399710-11
Produced by FastQC (version 0.11.5)