##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364615_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3124287 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.623412957900474 31.0 30.0 33.0 26.0 34.0 2 29.667208870375866 31.0 30.0 34.0 26.0 34.0 3 29.679733648029135 31.0 30.0 34.0 25.0 34.0 4 33.35252299164577 35.0 33.0 37.0 28.0 37.0 5 32.77741545511024 35.0 33.0 37.0 26.0 37.0 6 32.79178865449941 35.0 33.0 37.0 26.0 37.0 7 32.571962178890736 35.0 33.0 37.0 25.0 37.0 8 32.50264172273546 35.0 33.0 37.0 25.0 37.0 9 33.76258071041489 37.0 33.0 39.0 23.0 39.0 10-11 33.54963116384634 37.0 33.0 39.0 22.0 39.0 12-13 33.41009900818971 37.0 33.0 39.0 20.0 39.0 14-15 34.3564256420745 38.0 33.0 41.0 19.0 41.0 16-17 34.27686332913718 38.0 33.0 41.0 18.5 41.0 18-19 34.3243701682976 38.5 33.0 41.0 19.0 41.0 20-21 34.35399404728183 39.0 33.0 41.0 19.0 41.0 22-23 34.34747415970428 39.0 33.0 41.0 19.0 41.0 24-25 34.33899670548833 39.0 33.0 41.0 20.0 41.0 26-27 34.23767278742318 39.0 33.0 41.0 19.0 41.0 28-29 33.99131977952089 38.0 33.0 41.0 18.0 41.0 30-31 33.91439886924601 38.0 33.0 40.0 18.0 41.0 32-33 33.722335208001056 38.0 33.0 40.0 16.5 41.0 34-35 33.580873972205495 38.0 33.0 40.0 16.0 41.0 36-37 33.40789130448003 38.0 32.0 40.0 15.5 41.0 38-39 33.2635401293159 38.0 32.0 40.0 14.0 41.0 40-41 33.06804192444548 38.0 32.0 40.0 9.0 41.0 42-43 32.87868816149093 38.0 32.0 40.0 8.5 41.0 44-45 32.65174646247288 38.0 31.0 40.0 6.5 41.0 46-47 32.36204148338485 38.0 31.0 40.0 2.0 41.0 48-49 32.079682020249734 38.0 30.5 40.0 2.0 41.0 50-51 31.8693034602775 37.5 30.0 40.0 2.0 41.0 52-53 31.69428448794877 37.0 30.0 40.0 2.0 41.0 54-55 31.472191255156776 37.0 30.0 40.0 2.0 41.0 56-57 31.24907634925985 37.0 29.0 40.0 2.0 41.0 58-59 31.061535479935102 36.0 29.0 40.0 2.0 41.0 60-61 30.841686919287504 36.0 29.0 40.0 2.0 41.0 62-63 30.57480362719558 35.5 28.5 39.5 2.0 41.0 64-65 30.300869766445913 35.0 28.0 39.0 2.0 41.0 66-67 30.02109777366804 35.0 28.0 39.0 2.0 40.5 68-69 29.708768112532553 35.0 28.0 38.5 2.0 40.0 70-71 29.457165906973337 35.0 28.0 38.0 2.0 40.0 72-73 29.3038725315568 35.0 28.0 37.0 2.0 39.5 74-75 29.015782320894335 34.5 28.0 37.0 2.0 39.0 76-77 28.721590077992197 34.0 28.0 36.0 2.0 39.0 78-79 28.4200495985164 34.0 27.0 36.0 2.0 38.5 80-81 28.144930987454096 34.0 27.0 35.5 2.0 37.0 82-83 27.894310285834816 34.0 26.0 35.0 2.0 37.0 84-85 27.88414620679854 34.0 27.0 35.0 2.0 36.5 86-87 27.74493892526519 34.0 27.0 35.0 2.0 36.0 88-89 27.606244400722467 34.0 27.0 35.0 2.0 36.0 90-91 27.46154370581192 34.0 26.5 35.0 2.0 36.0 92-93 27.327328443257613 34.0 26.0 35.0 2.0 35.0 94-95 27.245319492095316 34.0 26.0 35.0 2.0 35.0 96-97 27.167814928654124 34.0 26.0 35.0 2.0 35.0 98-99 27.07991727392522 34.0 26.0 35.0 2.0 35.0 100-101 26.984143102090172 34.0 25.5 35.0 2.0 35.0 102-103 25.804936134228385 31.5 23.5 34.0 2.0 35.0 104-105 26.733729807792947 33.5 25.0 35.0 2.0 35.0 106-107 26.90829427642211 34.0 25.0 35.0 2.0 35.0 108-109 26.92051866553873 34.0 25.0 35.0 2.0 35.0 110-111 26.864900375669713 34.0 25.0 35.0 2.0 35.0 112-113 26.814012765152498 34.0 25.0 35.0 2.0 35.0 114-115 26.73731702625271 34.0 25.0 35.0 2.0 35.0 116-117 26.619318743764577 34.0 25.0 35.0 2.0 35.0 118-119 26.5134912381609 34.0 25.0 35.0 2.0 35.0 120-121 26.377949112869594 34.0 25.0 35.0 2.0 35.0 122-123 26.190956208568544 34.0 24.5 35.0 2.0 35.0 124-125 25.377807160481737 33.0 21.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.708910790590771 1101 2 0.7598031031577115 1101 3 0.6063280438113949 1101 4 0.6601324116405181 1101 5 0.9010662829172702 1101 6 0.9274929817804249 1101 7 0.8807358890951988 1101 8 0.9359997749662767 1101 9 1.0336877097627095 1101 10-11 1.0984531961367168 1101 12-13 1.0678904909118145 1101 14-15 1.1467260291601988 1101 16-17 1.3728849148116424 1101 18-19 1.3906048420598367 1101 20-21 1.2992891683337646 1101 22-23 1.2255095272121466 1101 24-25 1.1378943883963615 1101 26-27 1.1950768768568807 1101 28-29 1.3352633426537537 1101 30-31 1.2477767369361246 1101 32-33 1.3301171449597007 1101 34-35 1.3152068138271247 1101 36-37 1.2908228582872496 1101 38-39 1.3090122172194043 1101 40-41 1.3324309574185094 1101 42-43 1.3272947065200995 1101 44-45 1.2802416656407871 1101 46-47 1.3643720219506932 1101 48-49 1.4024992541691432 1101 50-51 1.3527524844352392 1101 52-53 1.3538788410226559 1101 54-55 1.2891171239732806 1101 56-57 1.3711861288108942 1101 58-59 1.3381725107968307 1101 60-61 1.3409187350265341 1101 62-63 1.305745148345956 1101 64-65 1.3724744484942946 1101 66-67 1.3377646358706237 1101 68-69 1.3336676083349275 1101 70-71 1.3791274351650813 1101 72-73 1.3332882774948729 1101 74-75 1.292605179983802 1101 76-77 1.3542413900260755 1101 78-79 1.2839506502288458 1101 80-81 1.248882050746328 1101 82-83 1.203673495244864 1101 84-85 1.288483597068641 1101 86-87 1.2523298601084356 1101 88-89 1.2775124632148156 1101 90-91 1.2759313167589035 1101 92-93 1.1884943250042213 1101 94-95 1.1367108308512286 1101 96-97 1.2325222253307153 1101 98-99 1.2778518465645448 1101 100-101 1.2467904775161536 1101 102-103 1.2840211804271497 1101 104-105 1.2052846867896 1101 106-107 1.1696711756977933 1101 108-109 1.155710983908964 1101 110-111 1.148883851177498 1101 112-113 1.1889203650003886 1101 114-115 1.1131894375745546 1101 116-117 1.1550979621732615 1101 118-119 1.2242119246438001 1101 120-121 1.2142468930639225 1101 122-123 1.2734950661039228 1101 124-125 1.0128004833904924 1102 1 0.4840634887130193 1102 2 -0.49588440188828997 1102 3 0.10003912874120857 1102 4 0.3841452114718109 1102 5 0.7067671016027646 1102 6 0.7498725897853618 1102 7 0.819801946699215 1102 8 0.7442374412960646 1102 9 1.0036885299820995 1102 10-11 1.1027941360787423 1102 12-13 1.2436700736212956 1102 14-15 1.3031411321822333 1102 16-17 1.445411551505245 1102 18-19 1.3169423438773151 1102 20-21 1.279390673468491 1102 22-23 1.3341620518759 1102 24-25 1.316761619492759 1102 26-27 1.3787376611207236 1102 28-29 1.406819332822515 1102 30-31 1.3811227562681339 1102 32-33 1.4652923775304743 1102 34-35 1.2669269140059427 1102 36-37 1.2030610597344165 1102 38-39 1.058437879946112 1102 40-41 1.0518926877544317 1102 42-43 1.043661945653362 1102 44-45 0.9054751824687344 1102 46-47 0.977497635565264 1102 48-49 1.0344403837260643 1102 50-51 0.9304902008691407 1102 52-53 0.9972615488690479 1102 54-55 0.9990911525024053 1102 56-57 0.8117340463859186 1102 58-59 0.9829700511673778 1102 60-61 0.7217302488860824 1102 62-63 0.8459441879452854 1102 64-65 0.8044155734587157 1102 66-67 0.8870548154380522 1102 68-69 0.7603299831630679 1102 70-71 0.6517765812230074 1102 72-73 0.7742746634144346 1102 74-75 0.9068640603283029 1102 76-77 0.8802041581278282 1102 78-79 0.8296234142882817 1102 80-81 0.8176707189699748 1102 82-83 0.787950808022444 1102 84-85 0.8630076930109851 1102 86-87 0.808445335058174 1102 88-89 0.9452692807488816 1102 90-91 0.853237315814134 1102 92-93 0.9920890260379238 1102 94-95 0.8660483746441017 1102 96-97 0.8311905077591604 1102 98-99 0.8934093004529231 1102 100-101 0.7161187959075015 1102 102-103 0.6226578720093237 1102 104-105 0.610539450330819 1102 106-107 0.8725731771157754 1102 108-109 0.9522013955626569 1102 110-111 0.9480125357008191 1102 112-113 0.9510923105396607 1102 114-115 1.0226984577375333 1102 116-117 1.0701787106138134 1102 118-119 0.9809625231146306 1102 120-121 1.0335063837508507 1102 122-123 1.0028290277516376 1102 124-125 0.8358523865612497 1103 1 0.4269628328917605 1103 2 -0.9457807261098523 1103 3 0.05547193357627478 1103 4 0.3980758419201962 1103 5 0.6105631141678458 1103 6 0.6330833821142505 1103 7 0.7013286500279392 1103 8 0.6697320128076996 1103 9 0.76898603662422 1103 10-11 0.9511543847383734 1103 12-13 0.9993180609487382 1103 14-15 1.0191893370059546 1103 16-17 1.0924247824676456 1103 18-19 1.1123810474947433 1103 20-21 1.0537975768967343 1103 22-23 1.0628004098259822 1103 24-25 0.8533583142848826 1103 26-27 0.9898526208669978 1103 28-29 1.0927675003259907 1103 30-31 1.014680329061612 1103 32-33 1.0446989391907024 1103 34-35 1.1020520059410828 1103 36-37 1.147302863167063 1103 38-39 1.2207260673140752 1103 40-41 1.2835895814172886 1103 42-43 1.2338177314909728 1103 44-45 1.2354377566811365 1103 46-47 1.2513343648466915 1103 48-49 1.239990871527894 1103 50-51 1.264785416444095 1103 52-53 1.3014057499921776 1103 54-55 1.2350000201403724 1103 56-57 1.1542862436731305 1103 58-59 1.1837891836131078 1103 60-61 1.157925823711551 1103 62-63 1.196719489737049 1103 64-65 1.1788825432503387 1103 66-67 1.157549200970724 1103 68-69 1.0889468256293924 1103 70-71 1.043112925525687 1103 72-73 0.9810959613281547 1103 74-75 0.8983900340295463 1103 76-77 0.8428619573547174 1103 78-79 0.8455493661790499 1103 80-81 0.9349675777508288 1103 82-83 0.9534141193982748 1103 84-85 1.0264212720006256 1103 86-87 0.9384333102626421 1103 88-89 0.8996757905562411 1103 90-91 1.0210466353463978 1103 92-93 0.9400363276676309 1103 94-95 0.9151485476114445 1103 96-97 0.9069505221728775 1103 98-99 0.9235716533987599 1103 100-101 0.9441062966466944 1103 102-103 0.9702888619202277 1103 104-105 0.9255158079576162 1103 106-107 0.9161153678132976 1103 108-109 1.0510157486027047 1103 110-111 0.9453335629886546 1103 112-113 0.9630380362108788 1103 114-115 0.9415530734932531 1103 116-117 0.9873348337946588 1103 118-119 1.031804284152365 1103 120-121 1.1219372113092732 1103 122-123 1.1297751292985474 1103 124-125 1.0143647143849464 1104 1 0.17472949588978182 1104 2 -0.8222849202560667 1104 3 -0.00957029008921495 1104 4 0.14055643338612356 1104 5 0.46223502780072323 1104 6 0.47475521914908825 1104 7 0.5874983082514831 1104 8 0.535838223659546 1104 9 0.5025615004153181 1104 10-11 0.7282347171416461 1104 12-13 0.5897591891008886 1104 14-15 0.7237992493576257 1104 16-17 0.9050304827330464 1104 18-19 0.7472220319651015 1104 20-21 0.8204297341275364 1104 22-23 0.708593503987963 1104 24-25 0.7409428190644007 1104 26-27 0.5652883932030832 1104 28-29 0.8044488234242095 1104 30-31 0.7503515557309512 1104 32-33 0.8308946407501949 1104 34-35 0.7700057213696638 1104 36-37 0.841455932672531 1104 38-39 0.7892815208732884 1104 40-41 0.8687486695135078 1104 42-43 0.9251886793031119 1104 44-45 0.7439072833576148 1104 46-47 0.7832267679733462 1104 48-49 0.7713305592739417 1104 50-51 0.8720732771453079 1104 52-53 0.6561083258232401 1104 54-55 0.8040612223830017 1104 56-57 0.8128061033293825 1104 58-59 0.7594160479188616 1104 60-61 0.73754767931133 1104 62-63 0.8144762877129246 1104 64-65 0.820383123178182 1104 66-67 0.8045926574616225 1104 68-69 0.8176522662274053 1104 70-71 0.8972583513523738 1104 72-73 0.7922642387711214 1104 74-75 0.9241162943798571 1104 76-77 0.8396831384079064 1104 78-79 0.8147400191209613 1104 80-81 0.796702862157364 1104 82-83 0.7693992045490958 1104 84-85 0.7204685152570747 1104 86-87 0.7564361492281471 1104 88-89 0.7602729892447222 1104 90-91 0.7811697727540228 1104 92-93 0.7881468352667618 1104 94-95 0.7938553387539713 1104 96-97 0.6991668302195819 1104 98-99 0.764762535328515 1104 100-101 0.7614653088705303 1104 102-103 0.762349958135033 1104 104-105 0.7124692254601079 1104 106-107 0.7200594415635067 1104 108-109 0.6934919457596287 1104 110-111 0.6040848293761023 1104 112-113 0.6277792698667746 1104 114-115 0.6384888694134823 1104 116-117 0.6607229790777716 1104 118-119 0.6510311599075109 1104 120-121 0.5910857353253789 1104 122-123 0.6628677028760102 1104 124-125 0.6137010930953686 1105 1 0.5270787587964989 1105 2 0.278341637638853 1105 3 0.512165947709633 1105 4 0.4395718362407237 1105 5 0.6729776300462333 1105 6 0.5344740765233524 1105 7 0.6360136412726689 1105 8 0.6700448308285729 1105 9 0.7084667087358341 1105 10-11 0.829216464824782 1105 12-13 0.9413412342937946 1105 14-15 0.8725238451603303 1105 16-17 1.0454782541405407 1105 18-19 0.9792878510894312 1105 20-21 1.054987148957217 1105 22-23 0.8554042757324325 1105 24-25 0.9719691673079751 1105 26-27 0.9721477828180483 1105 28-29 1.1463572032825695 1105 30-31 1.0343828670038349 1105 32-33 0.96537291824162 1105 34-35 0.9600926148578353 1105 36-37 0.9366480911250861 1105 38-39 0.9639403031642217 1105 40-41 0.884679753973515 1105 42-43 0.9317182868350145 1105 44-45 0.9877756615149309 1105 46-47 0.918617089256692 1105 48-49 0.9096969711943643 1105 50-51 0.9637244390675157 1105 52-53 0.8048717131689926 1105 54-55 0.7268604391262663 1105 56-57 0.6922601420427839 1105 58-59 0.6556701707345702 1105 60-61 0.6843447434153802 1105 62-63 0.699290747784076 1105 64-65 0.8547202443535085 1105 66-67 0.7987077191794647 1105 68-69 0.6978212177526579 1105 70-71 0.7112047421738232 1105 72-73 0.6034754912112419 1105 74-75 0.6796192212309222 1105 76-77 0.7188136336336548 1105 78-79 0.71861017171614 1105 80-81 0.5543321354602959 1105 82-83 0.6853750601055353 1105 84-85 0.5184252997079923 1105 86-87 0.6456904384783577 1105 88-89 0.6848458667245367 1105 90-91 0.6780597142170031 1105 92-93 0.5093589447042461 1105 94-95 0.6089251084850389 1105 96-97 0.6772330512907949 1105 98-99 0.735459636842549 1105 100-101 0.8038486206925874 1105 102-103 0.39874754274086044 1105 104-105 0.28602910831266826 1105 106-107 0.6372461242994163 1105 108-109 0.652768445704691 1105 110-111 0.6822508895606312 1105 112-113 0.5527108989626335 1105 114-115 0.6287164658942217 1105 116-117 0.3339380338230633 1105 118-119 0.2829533402296853 1105 120-121 0.22564977731349956 1105 122-123 0.3218542052754927 1105 124-125 0.13193216851048817 1106 1 0.39392714344282 1106 2 0.40745673065760357 1106 3 0.4811249420048824 1106 4 0.30162703680077385 1106 5 0.509079928331829 1106 6 0.6315770152903539 1106 7 0.6191213166230725 1106 8 0.6636415062832413 1106 9 0.6254558503418011 1106 10-11 0.8272235250151425 1106 12-13 0.6665992887198229 1106 14-15 0.7310936352909607 1106 16-17 0.9584301286657677 1106 18-19 0.8362549482191852 1106 20-21 0.8955460062192273 1106 22-23 0.8329926398237646 1106 24-25 0.8214106079442871 1106 26-27 0.8773356821879972 1106 28-29 0.9896515119350298 1106 30-31 0.8500620007155035 1106 32-33 0.8160253155784147 1106 34-35 0.7457071046324799 1106 36-37 0.7381763095441372 1106 38-39 0.8760655098311929 1106 40-41 0.8600845233257388 1106 42-43 0.8344604409378249 1106 44-45 0.7585890728976352 1106 46-47 0.647098403584117 1106 48-49 0.7026055015129629 1106 50-51 0.8174854054496841 1106 52-53 0.883598048870617 1106 54-55 0.7844359935895362 1106 56-57 0.5667518530218274 1106 58-59 0.5735217279980773 1106 60-61 0.710455531803305 1106 62-63 0.8078270243528891 1106 64-65 0.7469696746229602 1106 66-67 0.5937290933844466 1106 68-69 0.6520267973883271 1106 70-71 0.7026803815381086 1106 72-73 0.7692618683207328 1106 74-75 0.8070355490382397 1106 76-77 0.8603540658841276 1106 78-79 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-2.464387601283022 2216 70-71 -3.0535972770049007 2216 72-73 -3.433719764802561 2216 74-75 -3.030392967390952 2216 76-77 -3.2260363788900364 2216 78-79 -2.9067016019796412 2216 80-81 -2.588539071986819 2216 82-83 -2.510044330455095 2216 84-85 -2.417160884479813 2216 86-87 -2.5122408009822514 2216 88-89 -2.4244534953842596 2216 90-91 -2.3458700480479955 2216 92-93 -2.5816672470784354 2216 94-95 -2.346187152796986 2216 96-97 -2.394195147839806 2216 98-99 -2.4297669881708686 2216 100-101 -2.5868161111277423 2216 102-103 -2.384832875792384 2216 104-105 -2.2616226119004423 2216 106-107 -2.4357613628195907 2216 108-109 -2.4911004582521556 2216 110-111 -2.3159079728787866 2216 112-113 -2.3819641893598877 2216 114-115 -2.4321662898222556 2216 116-117 -2.479570342984676 2216 118-119 -2.586118605732505 2216 120-121 -2.5445938524096903 2216 122-123 -2.538191128207309 2216 124-125 -2.697752244842018 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 119432.0 3 38630.0 4 18366.0 5 14168.0 6 15345.0 7 18797.0 8 21139.0 9 22681.0 10 24377.0 11 27064.0 12 30650.0 13 33883.0 14 37362.0 15 30746.0 16 19929.0 17 16802.0 18 15194.0 19 14194.0 20 13989.0 21 14762.0 22 17461.0 23 22984.0 24 31706.0 25 42275.0 26 50441.0 27 55995.0 28 64566.0 29 74868.0 30 86466.0 31 102403.0 32 124394.0 33 153219.0 34 196756.0 35 265775.0 36 418919.0 37 634329.0 38 233553.0 39 667.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.46294965457254 19.818844339229873 13.635417427273206 24.082788578924376 2 15.719221346972873 20.957659172094978 38.59510093840169 24.728018542530457 3 19.253032771957248 25.604625951457084 29.371661438273755 25.77067983831191 4 12.887583313647275 17.480453182707762 36.235762817225655 33.396200686419306 5 13.051568757275808 39.1447810757888 33.4815922831921 14.322057883743296 6 31.572030401904055 37.45854242164109 16.903649419161802 14.065777757293043 7 27.973655420790543 33.54673675841456 20.400661143057967 18.07894667773693 8 26.443953452419706 36.03737428731739 18.8807878405537 18.6378844197092 9 26.638726282067083 16.06451553018288 19.63437230637163 37.662385881378405 10-11 24.749193014010604 27.61336475766721 26.60259458926263 21.034847639059564 12-13 25.81339628681178 24.74146927016645 27.70877080665813 21.736363636363638 14-15 22.721909685266837 25.66055552443523 26.179285068380242 25.43824972191769 16-17 21.9309451244409 29.100983876819893 26.621276756546607 22.3467942421926 18-19 21.79330979621094 27.123554208971683 29.583685480627647 21.49945051418973 20-21 22.680305449756695 26.132436358898826 29.850269695726627 21.336988495617852 22-23 22.41896678002909 26.1286518407736 29.56145579377599 21.89092558542132 24-25 22.031666745084557 26.257590931947032 29.78820447673341 21.922537846234995 26-27 21.83467780008687 26.628699604101673 29.665792547227575 21.870830048583883 28-29 21.781783153766046 26.478612043711003 29.830959953783463 21.908644848739485 30-31 21.872273877571725 26.247592850399602 29.88842412499622 21.991709147032452 32-33 21.856545122006647 26.21233644476908 29.749447373929875 22.18167105929439 34-35 22.16272064634267 26.207899594371455 29.74256206296029 21.886817696325593 36-37 22.247524104403794 26.128698399803984 29.48528673946119 22.13849075633103 38-39 21.974181629280537 26.383283609988457 29.53637101841156 22.10616374231945 40-41 22.144268436286424 26.213596894267393 29.519631199054373 22.122503470391806 42-43 22.061742881816745 26.275533883749407 29.246391535448318 22.416331698985527 44-45 22.218765433521313 26.4824742413229 28.9702098430778 22.32855048207799 46-47 22.378409243853348 26.821990912150522 28.323616165828845 22.475983678167285 48-49 22.48855414822506 26.8293358205229 28.114913663197488 22.56719636805455 50-51 22.550117747600037 26.993703324059158 27.903244520515607 22.552934407825195 52-53 22.746568054221694 27.00202238304482 27.71767423869369 22.533735324039792 54-55 22.461364784989343 27.012339135297108 27.832270210771288 22.69402586894226 56-57 22.50169921950331 27.195626056160744 27.767335199714104 22.53533952462184 58-59 22.545435573850224 26.756525049799368 27.850454815843555 22.847584560506856 60-61 22.59317085786293 26.816406431291362 28.114398581180282 22.476024129665426 62-63 22.479640954880264 26.883333701417317 28.105020441463925 22.532004902238494 64-65 22.404572987615147 26.76387796852342 28.035715343107704 22.795833700753732 66-67 22.31611564494555 27.061198283000248 27.867990360680693 22.754695711373508 68-69 22.338405825745827 26.846323596603945 28.066944929046617 22.74832564860362 70-71 22.21185185611949 26.942451189663437 28.000100502930746 22.845596451286326 72-73 22.47179161024562 26.935537329754606 27.747024161518286 22.845646898481483 74-75 22.350515873098434 27.05571832770277 27.680060252812787 22.913705546386005 76-77 22.509521013445205 26.88919520010012 27.778377917298215 22.82290586915646 78-79 22.46134434738299 27.063309873851406 27.692852561189046 22.78249321757656 80-81 22.319869471733412 27.21272698611097 27.727158153495544 22.740245388660078 82-83 22.54064529964456 27.16584754591851 27.52237711988503 22.771130034551906 84-85 22.402793343563943 26.991395950016717 27.57223293845359 23.033577767965756 86-87 22.34071206679701 26.854292272614593 27.832056208943428 22.972939451644965 88-89 22.417402754612493 26.926463541921724 27.75548469138719 22.900649012078595 90-91 22.34263369530392 27.137327652677236 27.7044170397918 22.815621612227048 92-93 22.3300629022619 26.858267129270853 27.76993540122399 23.041734567243257 94-95 22.262376407179225 26.852047762069276 27.73410254382986 23.15147328692164 96-97 22.445467040793528 26.773981666247344 27.661028515969853 23.119522776989278 98-99 22.242760438721913 26.675094270413734 27.62487208958802 23.457273201276337 100-101 22.027113533721476 26.895530795993945 27.773207821347068 23.30414784893751 102-103 22.307847173944495 26.59199675829726 27.67611715196186 23.424038915796388 104-105 22.215468642424835 26.467791345751596 27.971070992926666 23.3456690188969 106-107 22.111676686717438 26.694183462555042 27.777549271362396 23.416590579365124 108-109 22.113577477520302 26.684098906675896 27.47930481660858 23.723018799195223 110-111 22.019394007676688 26.734376480348654 27.721552980157888 23.524676531816773 112-113 22.082257323164104 26.674030694617112 27.75208405618598 23.49162792603281 114-115 21.89660806838852 26.65676461070709 27.536939064426953 23.90968825647744 116-117 21.69220590664142 26.590208987098187 27.77471567230393 23.942869433956464 118-119 21.768249355039018 26.735233114185302 27.516628150386335 23.979889380389345 120-121 21.729910532079476 26.658056738455905 27.673866077576427 23.938166651888192 122-123 21.70737546743024 26.64916493905923 27.56675734405202 24.076702249458513 124-125 21.642339002266485 26.47341529296423 27.640394197465547 24.24385150730374 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 175.0 1 149.5 2 253.5 3 475.5 4 430.0 5 432.5 6 1138.0 7 2422.0 8 3681.0 9 4313.0 10 3725.5 11 2818.5 12 2747.5 13 3021.0 14 3384.0 15 3834.5 16 4106.5 17 4387.5 18 4828.5 19 5212.0 20 5557.5 21 6289.5 22 7331.5 23 8792.0 24 10717.0 25 13233.0 26 17233.5 27 21674.5 28 25605.5 29 30326.0 30 36201.5 31 42072.0 32 48025.5 33 53996.0 34 59839.5 35 65151.5 36 72324.5 37 80629.5 38 85437.0 39 88359.5 40 90952.5 41 94782.5 42 99397.5 43 104516.0 44 111885.5 45 116392.0 46 115217.5 47 114966.0 48 118963.0 49 121327.5 50 120359.5 51 116692.0 52 111843.0 53 108742.5 54 105104.0 55 96910.5 56 86520.5 57 77941.0 58 72059.0 59 66495.0 60 57387.0 61 46199.5 62 35608.5 63 26600.5 64 19905.0 65 15221.5 66 11066.0 67 8192.5 68 6080.5 69 4168.5 70 3089.5 71 2282.0 72 1786.0 73 1606.0 74 1263.0 75 883.0 76 569.5 77 346.0 78 228.0 79 140.0 80 98.0 81 83.0 82 64.5 83 39.0 84 22.5 85 13.5 86 9.0 87 7.0 88 4.0 89 3.5 90 2.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 1.5 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.056940991656656385 2 0.07627340253952342 3 0.0 4 0.00796981839376472 5 0.029542740471666017 6 2.880657250758333E-4 7 3.20073027862037E-5 8 0.0 9 5.121168445792592E-4 10-11 0.059725626999056106 12-13 0.009186095899640461 14-15 0.006785548190675185 16-17 0.05249197656937407 18-19 8.001825696550925E-5 20-21 0.009714216395612823 22-23 0.012258796967116016 24-25 0.0 26-27 0.0 28-29 0.0510516479439949 30-31 1.600365139310185E-5 32-33 0.003616825214841018 34-35 0.0 36-37 4.3209858761374993E-4 38-39 0.0 40-41 0.0 42-43 0.0033607667925513885 44-45 0.0 46-47 1.7604016532412036E-4 48-49 3.520803306482407E-4 50-51 6.241424043309721E-4 52-53 8.001825696550925E-5 54-55 0.0 56-57 0.0018724272129929166 58-59 1.600365139310185E-5 60-61 0.0 62-63 0.0 64-65 8.802008266206018E-4 66-67 0.0 68-69 5.601277987585648E-4 70-71 0.0 72-73 0.0026726097826480087 74-75 0.0037608580773789346 76-77 4.3209858761374993E-4 78-79 0.0011202555975171296 80-81 0.0019044345157791204 82-83 6.40146055724074E-5 84-85 8.001825696550925E-5 86-87 4.961131931861574E-4 88-89 0.0 90-91 0.0 92-93 0.007489708851971666 94-95 0.006977592007392406 96-97 0.0068175554934613875 98-99 9.60219083586111E-4 100-101 5.441241473654629E-4 102-103 9.60219083586111E-5 104-105 5.761314501516666E-4 106-107 0.004304982224744397 108-109 0.006097391180771804 110-111 7.36167964082685E-4 112-113 0.0029126645535445367 114-115 6.40146055724074E-4 116-117 0.0024005477089652774 118-119 0.002144489286675648 120-121 0.011970731242040185 122-123 7.681752668688888E-4 124-125 0.002112481983889444 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3124287.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.87908244313304 #Duplication Level Percentage of deduplicated Percentage of total 1 73.31218589981037 33.63495820983696 2 12.162213903510947 11.159824287403953 3 5.204871028656267 7.163841210887867 4 2.691415919578256 4.939187714523661 5 1.6668286948392927 3.8236285554555876 6 1.0641972632022147 2.9294636384526576 7 0.7327209174993893 2.3531594367233795 8 0.5094305524764925 1.8697765052895852 9 0.3907195614804163 1.6133269475974326 >10 2.0260698834865027 16.675793187615852 >50 0.15140364024590133 4.790587500435954 >100 0.08398876529552003 6.832248770211496 >500 0.0031772972560187075 0.967793070823005 >1k 5.648528454509085E-4 0.3805283572740026 >5k 1.4121321136272713E-4 0.5044017239391755 >10k+ 7.060660568136356E-5 0.3614808835294624 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11159 0.35716949179124713 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9434 0.3019568944850457 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6137 0.1964288171989321 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.20073027862037E-5 0.0 3 0.0 0.0 0.0 3.20073027862037E-5 0.0 4 0.0 0.0 0.0 3.20073027862037E-5 0.0 5 0.0 0.0 0.0 3.20073027862037E-5 0.0 6 0.0 0.0 0.0 9.60219083586111E-5 0.0 7 0.0 0.0 0.0 9.60219083586111E-5 0.0 8 0.0 0.0 0.0 9.60219083586111E-5 0.0 9 0.0 0.0 0.0 1.280292111448148E-4 0.0 10-11 0.0 0.0 0.0 1.280292111448148E-4 0.0 12-13 0.0 0.0 0.0 1.280292111448148E-4 0.0 14-15 0.0 0.0 0.0 1.4403286253791665E-4 0.0 16-17 0.0 0.0 0.0 1.920438167172222E-4 0.0 18-19 0.0 0.0 0.0 2.240511195034259E-4 0.0 20-21 0.0 0.0 0.0 2.7206207368273146E-4 0.0 22-23 0.0 0.0 0.0 4.961131931861574E-4 1.600365139310185E-5 24-25 0.0 0.0 0.0 6.721533585102777E-4 3.20073027862037E-5 26-27 0.0 0.0 0.0 8.001825696550925E-4 3.20073027862037E-5 28-29 0.0 0.0 0.0 0.0014563322767722684 3.20073027862037E-5 30-31 0.0 0.0 0.0 0.003184726627227268 3.20073027862037E-5 32-33 0.0 0.0 0.0 0.007073613915751018 3.20073027862037E-5 34-35 0.0 0.0 0.0 0.012146771407364304 3.20073027862037E-5 36-37 0.0 0.0 0.0 0.021076808884715137 3.20073027862037E-5 38-39 0.0 0.0 0.0 0.03570414625801023 3.20073027862037E-5 40-41 0.0 0.0 0.0 0.054028327103111846 3.20073027862037E-5 42-43 0.0 0.0 0.0 0.07136028156184115 3.20073027862037E-5 44-45 0.0 0.0 0.0 0.09181294804222531 3.20073027862037E-5 46-47 1.600365139310185E-5 0.0 0.0 0.11665061500431939 3.20073027862037E-5 48-49 3.20073027862037E-5 0.0 0.0 0.1452651436951855 3.20073027862037E-5 50-51 3.20073027862037E-5 0.0 0.0 0.1771764245730306 3.20073027862037E-5 52-53 3.20073027862037E-5 0.0 0.0 0.20783942064221372 3.20073027862037E-5 54-55 3.20073027862037E-5 0.0 0.0 0.24189519080673444 3.20073027862037E-5 56-57 3.20073027862037E-5 0.0 0.0 0.28233641787710284 3.20073027862037E-5 58-59 3.20073027862037E-5 0.0 0.0 0.3303953830105877 3.20073027862037E-5 60-61 3.20073027862037E-5 0.0 0.0 0.38459975027902366 3.20073027862037E-5 62-63 3.20073027862037E-5 0.0 0.0 0.44072455571463187 3.20073027862037E-5 64-65 3.20073027862037E-5 0.0 0.0 0.4944168061384886 3.20073027862037E-5 66-67 3.20073027862037E-5 0.0 0.0 0.5588154993443304 3.20073027862037E-5 68-69 3.20073027862037E-5 0.0 0.0 0.6359851063618676 3.20073027862037E-5 70-71 3.20073027862037E-5 0.0 0.0 0.724197232840645 3.20073027862037E-5 72-73 3.20073027862037E-5 0.0 0.0 0.8154660567355048 3.20073027862037E-5 74-75 3.20073027862037E-5 0.0 0.0 0.9109758482495366 3.20073027862037E-5 76-77 3.20073027862037E-5 0.0 0.0 1.0132711879542435 3.20073027862037E-5 78-79 3.20073027862037E-5 0.0 0.0 1.1374115118105348 3.20073027862037E-5 80-81 3.20073027862037E-5 0.0 0.0 1.2769633519583827 3.20073027862037E-5 82-83 3.20073027862037E-5 0.0 0.0 1.43157462806714 3.20073027862037E-5 84-85 3.20073027862037E-5 1.600365139310185E-5 0.0 1.5850016339728072 3.20073027862037E-5 86-87 6.40146055724074E-5 3.20073027862037E-5 0.0 1.7469265787682118 3.20073027862037E-5 88-89 6.40146055724074E-5 3.20073027862037E-5 0.0 1.934889464380193 3.20073027862037E-5 90-91 6.40146055724074E-5 3.20073027862037E-5 0.0 2.1538514227406127 3.20073027862037E-5 92-93 6.40146055724074E-5 3.20073027862037E-5 0.0 2.3902093501653336 3.20073027862037E-5 94-95 6.40146055724074E-5 3.20073027862037E-5 0.0 2.6324566213027163 3.20073027862037E-5 96-97 6.40146055724074E-5 3.20073027862037E-5 0.0 2.877056429194885 3.20073027862037E-5 98-99 6.40146055724074E-5 3.20073027862037E-5 0.0 3.146029798158748 6.40146055724074E-5 100-101 9.60219083586111E-5 3.20073027862037E-5 0.0 3.4655907091762055 6.40146055724074E-5 102-103 1.280292111448148E-4 3.20073027862037E-5 0.0 3.816678813438074 6.40146055724074E-5 104-105 1.280292111448148E-4 3.20073027862037E-5 0.0 4.171511772125928 9.60219083586111E-5 106-107 1.280292111448148E-4 3.20073027862037E-5 0.0 4.521751682863963 9.60219083586111E-5 108-109 1.280292111448148E-4 3.20073027862037E-5 0.0 4.895725008617966 9.60219083586111E-5 110-111 1.280292111448148E-4 3.20073027862037E-5 0.0 5.32092602248129 9.60219083586111E-5 112-113 1.280292111448148E-4 3.20073027862037E-5 0.0 5.783767624421188 9.60219083586111E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8210 0.0 54.70956 1 GTATCAA 13740 0.0 47.47472 1 ATCAACG 17070 0.0 37.890484 3 TATCAAC 17355 0.0 37.25783 2 TCAACGC 17445 0.0 37.009544 4 CAACGCA 17660 0.0 36.08822 5 AACGCAG 18145 0.0 35.144047 6 GTACATG 16160 0.0 32.73457 1 TACATGG 16345 0.0 31.43679 2 ACATGGG 16700 0.0 29.501764 3 ACGCAGA 22115 0.0 28.593729 7 CGCAGAG 22090 0.0 28.46436 8 GCAGAGT 23685 0.0 25.416222 9 CATGGGA 11035 0.0 25.398237 4 GAGTACT 13790 0.0 24.20473 12-13 GTACTTT 14470 0.0 23.170145 14-15 AGTACTT 14330 0.0 22.648487 12-13 CATGGGG 6220 0.0 22.290539 4 AGTACAT 12445 0.0 22.199104 2 CAGAGTA 23445 0.0 21.903997 10-11 >>END_MODULE