FastQCFastQC Report
Thu 26 May 2016
SRR1364614_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364614_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3061995
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46590.15215570240970347No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30840.1007186491160175No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA113950.042.713351
TCAACGC133400.035.056594
ATCAACG134750.034.9706423
CAACGCA136150.034.3053175
GTACATG165050.034.2897221
TATCAAC142650.033.7439162
AACGCAG137950.032.963736
TACATGG171000.032.5391852
ACATGGG173750.029.9326613
ACGCAGA157650.028.0084657
GGTATCA75600.027.3440951
CGCAGAG154650.026.4319578
CATGGGA114650.025.4091844
CATGGGG62550.025.2391344
CGCAGAT10850.024.6777348
ACGCATA3401.8189894E-1224.5035217
GCAGAGT170000.022.7152929
CGCATAG3705.456968E-1222.51388
GAGTACT52850.022.17727712-13
GTTATCA22600.021.8789041
Produced by FastQC (version 0.11.5)