##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364614_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3061995 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.019949412066318 31.0 26.0 31.0 7.0 34.0 2 25.830981435306068 31.0 25.0 31.0 7.0 34.0 3 26.23655753846757 31.0 26.0 31.0 7.0 34.0 4 29.449729343124336 35.0 28.0 37.0 7.0 37.0 5 29.83083283937433 35.0 30.0 37.0 7.0 37.0 6 29.785133548552498 35.0 30.0 37.0 2.0 37.0 7 29.67283421429493 35.0 30.0 37.0 2.0 37.0 8 29.416411195968642 35.0 29.0 37.0 2.0 37.0 9 30.239281906077572 37.0 28.0 39.0 2.0 39.0 10-11 30.187017287748674 36.0 28.0 39.0 2.0 39.0 12-13 30.181623418718843 37.0 28.0 39.0 2.0 39.0 14-15 30.774124059640855 37.0 27.5 40.0 2.0 41.0 16-17 31.162769044364865 37.0 29.0 40.0 2.0 41.0 18-19 30.987274799599607 37.0 28.0 40.0 2.0 41.0 20-21 30.90142897032817 37.0 27.5 40.0 2.0 41.0 22-23 30.964903110553742 37.0 28.0 40.0 2.0 41.0 24-25 31.058809371014647 37.0 28.5 40.0 2.0 41.0 26-27 30.864808237766553 37.0 27.5 40.0 2.0 41.0 28-29 30.759385955888234 37.0 28.0 40.0 2.0 41.0 30-31 30.621330374478077 37.0 27.5 40.0 2.0 41.0 32-33 30.511929640642784 37.0 28.0 40.0 2.0 41.0 34-35 30.5565360165513 37.0 28.0 40.0 2.0 41.0 36-37 30.489773170759587 37.0 28.0 40.0 2.0 41.0 38-39 30.188804847819803 37.0 27.0 40.0 2.0 41.0 40-41 30.041724594586206 36.0 27.0 40.0 2.0 41.0 42-43 29.520516036113712 35.5 25.5 40.0 2.0 41.0 44-45 29.35702491349594 35.0 25.0 40.0 2.0 41.0 46-47 29.295124747101156 35.0 25.5 40.0 2.0 41.0 48-49 29.214874289474672 35.0 26.0 40.0 2.0 41.0 50-51 28.974537515573996 35.0 24.0 40.0 2.0 41.0 52-53 28.862082400526454 35.0 24.5 40.0 2.0 41.0 54-55 28.6835954990129 35.0 24.0 39.0 2.0 41.0 56-57 28.456061489323137 35.0 23.5 39.0 2.0 40.5 58-59 28.237219525178844 34.0 23.0 39.0 2.0 40.0 60-61 27.93571707334597 34.0 22.5 39.0 2.0 40.0 62-63 27.75673213052275 34.0 22.5 38.5 2.0 40.0 64-65 27.529106024013757 34.0 22.0 38.0 2.0 40.0 66-67 27.26645699943991 34.0 22.0 38.0 2.0 40.0 68-69 27.037306396646628 33.0 21.5 37.5 2.0 40.0 70-71 26.619896342090698 33.0 20.0 37.0 2.0 39.5 72-73 26.405946613237447 33.0 20.0 36.0 2.0 39.0 74-75 26.382777731511645 33.0 20.0 36.0 2.0 39.0 76-77 26.130851291396624 33.0 19.5 36.0 2.0 39.0 78-79 25.84926069441655 33.0 18.5 35.0 2.0 37.5 80-81 25.44778469592537 32.0 17.0 35.0 2.0 37.0 82-83 25.556509236625143 32.5 18.0 35.0 2.0 37.0 84-85 25.384877016454958 33.0 18.0 35.0 2.0 36.0 86-87 25.249972975135492 33.0 17.5 35.0 2.0 36.0 88-89 25.08205565325874 32.5 15.5 35.0 2.0 36.0 90-91 24.94697019426877 32.0 12.5 35.0 2.0 35.0 92-93 24.817463287823788 32.0 10.0 35.0 2.0 35.0 94-95 24.710353870597437 32.0 7.0 35.0 2.0 35.0 96-97 24.595319881319206 32.0 7.0 35.0 2.0 35.0 98-99 24.50278086672251 32.0 6.0 35.0 2.0 35.0 100-101 24.390902826425254 32.0 2.0 35.0 2.0 35.0 102-103 23.288273658186903 30.0 2.0 34.0 2.0 35.0 104-105 24.10031956289935 32.0 2.0 34.5 2.0 35.0 106-107 24.256936082521364 32.0 2.0 35.0 2.0 35.0 108-109 24.210857790427482 32.0 2.0 35.0 2.0 35.0 110-111 24.135678046502363 32.0 2.0 35.0 2.0 35.0 112-113 24.03713379675669 32.0 2.0 35.0 2.0 35.0 114-115 23.927218365803995 32.0 2.0 35.0 2.0 35.0 116-117 23.807679470410633 32.0 2.0 35.0 2.0 35.0 118-119 23.676321319923773 32.0 2.0 35.0 2.0 35.0 120-121 23.484020711986794 32.0 2.0 35.0 2.0 35.0 122-123 23.263499940398333 32.0 2.0 35.0 2.0 35.0 124-125 22.33209410857954 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2429854376403249 1101 2 1.5015740199457 1101 3 1.0510587788901198 1101 4 1.1530248182321543 1101 5 1.023132524296848 1101 6 0.8003638337807928 1101 7 0.7911263737795551 1101 8 1.0920375990993065 1101 9 1.3587370061977104 1101 10-11 1.346251095917303 1101 12-13 1.36160548306718 1101 14-15 1.6513202330060075 1101 16-17 1.2557419739130857 1101 18-19 1.2211910176654541 1101 20-21 1.524580837567445 1101 22-23 1.2389210215756705 1101 24-25 1.1833444829354853 1101 26-27 1.4217980159559858 1101 28-29 1.3606471437168999 1101 30-31 1.5351788113662508 1101 32-33 1.4611213705844506 1101 34-35 1.2764721834396369 1101 36-37 1.2479484970687444 1101 38-39 1.209471940315737 1101 40-41 1.2804366565815748 1101 42-43 1.4785987222729666 1101 44-45 1.2422445861328697 1101 46-47 1.0432726988226797 1101 48-49 0.8797165634299837 1101 50-51 0.8554759838065387 1101 52-53 0.7394659925176086 1101 54-55 0.7185868798435564 1101 56-57 0.7895489353625678 1101 58-59 0.7562742366445967 1101 60-61 0.7367148624694799 1101 62-63 0.6352396486763041 1101 64-65 0.5937969515869561 1101 66-67 0.6204946004352578 1101 68-69 0.5635358024705006 1101 70-71 0.4794236702277779 1101 72-73 0.47846053706873093 1101 74-75 0.4626654411333426 1101 76-77 0.505523497396311 1101 78-79 0.5419445652057533 1101 80-81 0.6953412179352583 1101 82-83 0.6934864215167664 1101 84-85 0.7541469963271865 1101 86-87 0.7253250061826506 1101 88-89 0.7802648564668182 1101 90-91 0.8192939931249406 1101 92-93 0.7494922160309123 1101 94-95 0.712995191033361 1101 96-97 0.6860006041362467 1101 98-99 0.7585143136019852 1101 100-101 0.7316196356694675 1101 102-103 0.6215300307777412 1101 104-105 0.8387215327499504 1101 106-107 0.786464843874402 1101 108-109 0.7790696734454663 1101 110-111 0.8688630215052591 1101 112-113 0.8231274106224298 1101 114-115 0.7325809240262906 1101 116-117 0.5999726345284735 1101 118-119 0.6240311973751318 1101 120-121 0.7046810417494953 1101 122-123 0.7123538284199853 1101 124-125 0.5746165347857293 1102 1 0.6228340084120916 1102 2 0.9800970808009879 1102 3 0.6210660980729017 1102 4 0.4898550352004989 1102 5 0.49434876421479146 1102 6 0.13908783718083484 1102 7 0.31163079917523007 1102 8 0.2897168587226169 1102 9 0.8936474429218748 1102 10-11 0.9098317091676726 1102 12-13 0.9997648991148758 1102 14-15 1.32103144475494 1102 16-17 0.9334861560159844 1102 18-19 0.7221207012516295 1102 20-21 1.0591554926016293 1102 22-23 1.1136682218113165 1102 24-25 0.8337759647098082 1102 26-27 1.172766021266824 1102 28-29 0.7716457744121108 1102 30-31 0.8792868595295538 1102 32-33 1.1404883480489474 1102 34-35 1.1156885034429607 1102 36-37 0.9053837891035528 1102 38-39 1.141804597512614 1102 40-41 1.253703775339556 1102 42-43 1.3551040679717694 1102 44-45 0.9346497971893442 1102 46-47 0.7760619541576439 1102 48-49 0.7640407529073308 1102 50-51 0.858933204496914 1102 52-53 0.742447766889569 1102 54-55 0.7767094469230571 1102 56-57 0.6669266880913121 1102 58-59 0.7309831103077862 1102 60-61 0.7834370436848452 1102 62-63 0.5320010678473288 1102 64-65 0.3664429093648991 1102 66-67 0.5129037769782343 1102 68-69 0.3646935112259726 1102 70-71 0.4455583912810148 1102 72-73 0.09036404524446695 1102 74-75 0.22162785268432827 1102 76-77 -0.013540772862548067 1102 78-79 0.16096670338250263 1102 80-81 0.20804653002922535 1102 82-83 0.3096396174710385 1102 84-85 0.25525650842400793 1102 86-87 0.34432532933586657 1102 88-89 0.23909149615912995 1102 90-91 0.39259530814425503 1102 92-93 0.39732029289697124 1102 94-95 0.44523463505857563 1102 96-97 0.1884006445473716 1102 98-99 0.3560239056678327 1102 100-101 0.29448032890437403 1102 102-103 0.20312236364048175 1102 104-105 0.33870492909315075 1102 106-107 0.29539790209928185 1102 108-109 0.36514273942485787 1102 110-111 0.3820410893980757 1102 112-113 0.2660468017835491 1102 114-115 0.35312705974695646 1102 116-117 0.41253203804949834 1102 118-119 0.4731183307482141 1102 120-121 0.5370767249732467 1102 122-123 0.40567008333697174 1102 124-125 0.3653934433991033 1103 1 0.7689403636893815 1103 2 1.0962809886913902 1103 3 0.7782742648106549 1103 4 0.7104971504952111 1103 5 0.7527625001306397 1103 6 0.7070338000151537 1103 7 0.7984227369911174 1103 8 0.9149195233496066 1103 9 1.39582063347574 1103 10-11 1.3770207958470024 1103 12-13 1.2441914226470203 1103 14-15 1.4744705312503719 1103 16-17 1.2041093982017905 1103 18-19 1.1233321993861196 1103 20-21 1.5364219906583862 1103 22-23 1.2877279636236523 1103 24-25 1.113735940085757 1103 26-27 1.3560703007522363 1103 28-29 1.3798188726723772 1103 30-31 1.3808236803348706 1103 32-33 1.496104577735835 1103 34-35 1.2036934890626654 1103 36-37 1.0867632419692583 1103 38-39 1.1105444324051525 1103 40-41 1.238428277725287 1103 42-43 1.4519220868205558 1103 44-45 1.2338331508768015 1103 46-47 1.0081766491079307 1103 48-49 0.9671710653368493 1103 50-51 0.9429079219360439 1103 52-53 0.8072275493150158 1103 54-55 0.902317894425579 1103 56-57 0.8859768123909788 1103 58-59 0.7678103644011323 1103 60-61 0.936541308428783 1103 62-63 0.7862388617616105 1103 64-65 0.6787038538677592 1103 66-67 0.6385380931697249 1103 68-69 0.6691436564265558 1103 70-71 0.7817042028067966 1103 72-73 0.743283281432074 1103 74-75 0.6962139377228276 1103 76-77 0.6473062479496576 1103 78-79 0.6228664155510408 1103 80-81 0.6675600701914313 1103 82-83 0.6164740711515435 1103 84-85 0.7466290677132008 1103 86-87 0.6676974029312319 1103 88-89 0.7862649507943011 1103 90-91 0.8925152500689251 1103 92-93 0.8246915088778657 1103 94-95 0.7518063560355159 1103 96-97 0.7616708004122259 1103 98-99 0.7004188375233298 1103 100-101 0.634847714308659 1103 102-103 0.5429312550840386 1103 104-105 0.5515270990941339 1103 106-107 0.6934370652837316 1103 108-109 0.6993034294435709 1103 110-111 0.6680396259413612 1103 112-113 0.7271727336509635 1103 114-115 0.7494334376121472 1103 116-117 0.7827225154589748 1103 118-119 0.8819268620898093 1103 120-121 0.7192708086005624 1103 122-123 0.6528784104360739 1103 124-125 0.6083757339643618 1104 1 1.126991585254622 1104 2 1.4106073863068325 1104 3 0.9922374758474568 1104 4 1.0813807644973963 1104 5 0.7003278842866649 1104 6 0.8581251026809227 1104 7 0.6883001433057814 1104 8 0.9258284069773239 1104 9 1.3641531696329636 1104 10-11 1.357293330072718 1104 12-13 1.3372853774234201 1104 14-15 1.3822811856376696 1104 16-17 1.062217907871048 1104 18-19 1.0383842272707042 1104 20-21 1.4298395446477805 1104 22-23 1.2175179634350712 1104 24-25 1.1988662769512395 1104 26-27 1.5227961841748083 1104 28-29 1.5683215216400193 1104 30-31 1.5722825954781854 1104 32-33 1.5652697486258234 1104 34-35 1.4622954907461647 1104 36-37 1.3608467465954526 1104 38-39 1.376742108079629 1104 40-41 1.5371494529514536 1104 42-43 1.8486558505718413 1104 44-45 1.5191620531681522 1104 46-47 1.2310725899100312 1104 48-49 1.094842391055007 1104 50-51 1.1678246613200294 1104 52-53 1.1158214220879934 1104 54-55 1.204120412473479 1104 56-57 1.0681384111319545 1104 58-59 0.9980797556385923 1104 60-61 0.9169030606618769 1104 62-63 0.9292968293225456 1104 64-65 0.8485054370195115 1104 66-67 0.7639908263408763 1104 68-69 0.7045693484697075 1104 70-71 0.7869167059569264 1104 72-73 0.7230265792953539 1104 74-75 0.6936483307003627 1104 76-77 0.6694845302437216 1104 78-79 0.7265391687573164 1104 80-81 0.757012015901168 1104 82-83 0.8563285765109114 1104 84-85 0.85629864250118 1104 86-87 0.7517269081620199 1104 88-89 0.7837472743426552 1104 90-91 0.7067771449936835 1104 92-93 0.7547130183465462 1104 94-95 0.7931060073923994 1104 96-97 0.8005492079115122 1104 98-99 0.8382333720724837 1104 100-101 0.777931754385758 1104 102-103 0.7516921776825214 1104 104-105 0.8437629323603204 1104 106-107 0.7579488992059424 1104 108-109 0.7965325567157358 1104 110-111 0.7936782240085947 1104 112-113 0.8454505960989387 1104 114-115 0.8586482077139834 1104 116-117 0.805699473926385 1104 118-119 0.8391627210713537 1104 120-121 0.7976892226258343 1104 122-123 0.7187444437048924 1104 124-125 0.545333032754538 1105 1 0.7655890672615335 1105 2 1.0663831462048883 1105 3 0.518853957904863 1105 4 0.7060425122109422 1105 5 0.49190933811058457 1105 6 0.37612951815423656 1105 7 0.5150934373706626 1105 8 0.36725116812591097 1105 9 0.6650762901978098 1105 10-11 0.5695420914232905 1105 12-13 0.8356567724931807 1105 14-15 1.0797071822751967 1105 16-17 0.6964155110695565 1105 18-19 0.7475256902434069 1105 20-21 0.6314202707280359 1105 22-23 0.7808227513943322 1105 24-25 0.4309708373792098 1105 26-27 0.8085762028585108 1105 28-29 0.8800410536859893 1105 30-31 0.7769096342743467 1105 32-33 0.9084504562897031 1105 34-35 0.678243491975902 1105 36-37 0.3209572967993708 1105 38-39 0.505464461246202 1105 40-41 0.6811961013205625 1105 42-43 0.8750885402233273 1105 44-45 0.5183912389566565 1105 46-47 0.08610326653342071 1105 48-49 0.29195232044901687 1105 50-51 0.4115225311103501 1105 52-53 0.18934497499198955 1105 54-55 0.2937312775379226 1105 56-57 0.38611941627962665 1105 58-59 0.34978369689037336 1105 60-61 0.29775805550826107 1105 62-63 0.1549370024067187 1105 64-65 -0.1841488112212808 1105 66-67 -0.13951927444419354 1105 68-69 -0.19944785227169604 1105 70-71 0.0820284591656808 1105 72-73 0.032644008904721744 1105 74-75 0.012338700275329018 1105 76-77 0.054459538189490786 1105 78-79 0.13261642246064298 1105 80-81 0.24268818259308134 1105 82-83 0.22413022626125212 1105 84-85 0.11498422604763547 1105 86-87 0.21660677847512844 1105 88-89 0.2209288864879504 1105 90-91 0.14470265226072598 1105 92-93 0.18542801536131392 1105 94-95 0.07217333333542442 1105 96-97 0.16483596713528925 1105 98-99 0.2928026740623437 1105 100-101 0.32520446073079157 1105 102-103 0.1666463261184674 1105 104-105 0.25747286190285124 1105 106-107 0.2782680779249347 1105 108-109 0.313108125469693 1105 110-111 0.31928681821575466 1105 112-113 0.20119230798967536 1105 114-115 0.3262345604409447 1105 116-117 0.28176775336212856 1105 118-119 0.28578513646743886 1105 120-121 0.18118627971955448 1105 122-123 0.22633794762727888 1105 124-125 0.2018131445597291 1106 1 0.23442555976259882 1106 2 -1.753831714248765 1106 3 -0.2468114015033933 1106 4 0.056635433529336865 1106 5 0.2937482295775844 1106 6 0.27767750613342557 1106 7 0.4218749546516598 1106 8 0.3656604729237678 1106 9 0.7261466266664947 1106 10-11 0.9406135275984582 1106 12-13 1.00652916991465 1106 14-15 1.0736234553690345 1106 16-17 0.6550556766443911 1106 18-19 0.7116733193881011 1106 20-21 1.0350125038953983 1106 22-23 0.6029020608905284 1106 24-25 0.6630020484744996 1106 26-27 0.6892551854559059 1106 28-29 0.8477809971904868 1106 30-31 0.8784445683136077 1106 32-33 1.02261789638953 1106 34-35 0.9499053415252305 1106 36-37 0.6378040693457905 1106 38-39 0.9137533387120165 1106 40-41 1.0383163083504598 1106 42-43 1.235042318269901 1106 44-45 1.039394279523286 1106 46-47 0.8899248773625423 1106 48-49 0.7068888514172578 1106 50-51 0.6729171907769036 1106 52-53 0.550874630620573 1106 54-55 0.5856038422158178 1106 56-57 0.589941735287244 1106 58-59 0.5162764839345115 1106 60-61 0.6683640109195395 1106 62-63 0.6092780057792737 1106 64-65 0.5174513158358671 1106 66-67 0.47251303004260947 1106 68-69 0.4641096025654612 1106 70-71 0.581689841587842 1106 72-73 0.33141585598579937 1106 74-75 0.4301946181576568 1106 76-77 0.3959260459475189 1106 78-79 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-1.6345414733373183 2216 66-67 -1.7468151788810538 2216 68-69 -1.772994098005487 2216 70-71 -3.969945260970835 2216 72-73 -2.045198311914394 2216 74-75 -1.6190945761751827 2216 76-77 -1.4391498474760382 2216 78-79 -1.5307938164472077 2216 80-81 -1.3535198378847326 2216 82-83 -1.4729960536022304 2216 84-85 -1.5010055350445164 2216 86-87 -1.6089727437091739 2216 88-89 -1.5966810111229677 2216 90-91 -1.4585970064423464 2216 92-93 -1.5388619163793003 2216 94-95 -1.706193731510993 2216 96-97 -1.6867602785984985 2216 98-99 -1.7555700221833916 2216 100-101 -1.8517764266412193 2216 102-103 -1.615209475929312 2216 104-105 -1.6489658752985115 2216 106-107 -1.5959781678088518 2216 108-109 -1.7644384615610242 2216 110-111 -1.6116012363917527 2216 112-113 -1.8212326066860918 2216 114-115 -1.6986819924600773 2216 116-117 -1.477125591347768 2216 118-119 -1.5776950683107174 2216 120-121 -1.7284650854681587 2216 122-123 -1.6656257042498552 2216 124-125 -1.6184151830853217 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 318373.0 3 41081.0 4 24268.0 5 19279.0 6 18848.0 7 21507.0 8 23234.0 9 24692.0 10 26589.0 11 28358.0 12 29043.0 13 29712.0 14 29487.0 15 25383.0 16 20510.0 17 18038.0 18 16355.0 19 15118.0 20 14857.0 21 15982.0 22 19917.0 23 28477.0 24 41596.0 25 53043.0 26 57744.0 27 62307.0 28 71231.0 29 80674.0 30 92917.0 31 107774.0 32 128052.0 33 159919.0 34 207313.0 35 278297.0 36 389640.0 37 421511.0 38 100757.0 39 112.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.68835472071596 19.210940425164235 14.046548649858742 23.054156204261066 2 13.183308799262406 20.7946878061302 42.403845650135416 23.618157744471976 3 18.72009875028992 27.284124606721992 28.028224290002974 25.967552352985113 4 11.957292902480264 17.58772243007001 37.24014750168851 33.21483716576121 5 13.091640797485102 39.811310615637375 33.34873360608199 13.74831498079553 6 30.378279496605064 38.481696559955665 17.6415745053768 13.498449438062474 7 27.942583042933748 34.292792176461695 20.151614442750308 17.61301033785425 8 25.64378725296821 36.94512651895894 19.474550084450698 17.93653614362215 9 26.095075461676494 18.116431261754837 19.706498471523474 36.0819948050452 10-11 23.79568334499879 29.0865293413487 26.89740830624113 20.22037900741138 12-13 24.65911377269017 26.228978314601044 27.371792460083128 21.740115452625655 14-15 21.971204358938365 27.227560625975695 25.540605407379267 25.26062960770667 16-17 21.797517281806964 30.55273322752057 25.77094261903609 21.878806871636378 18-19 21.828473341539663 28.968126202073307 27.861597066663002 21.341803389724028 20-21 22.942898452946952 27.80327497679404 27.86902383154682 21.384802738712185 22-23 22.613310363741206 27.614383790728862 27.575979322130856 22.196326523399073 24-25 22.237085872320556 27.68965483953884 27.906397617874646 22.16686167026596 26-27 22.24981297258186 27.81186924287045 27.85156652855831 22.086751255989377 28-29 22.059188943152737 27.801299987649546 27.99051550472875 22.14899556446897 30-31 22.1289671237344 27.641354171317566 28.085746934577998 22.14393177037003 32-33 22.038947639505764 27.58372276500688 27.852297273285647 22.525032322201714 34-35 22.448789678288787 27.48353501273205 27.797968432363124 22.269706876616038 36-37 22.504540826222673 27.38982547461569 27.670076143269323 22.435557555892313 38-39 22.299592227371072 27.46241088920609 27.854805132127332 22.383191751295506 40-41 22.376057562282124 27.433424138928636 27.92504414827537 22.265474150513874 42-43 22.116937339183977 27.38045374475163 27.94721713982369 22.555391776240704 44-45 22.366170356400858 27.522621531074886 27.627981750648132 22.48322636187612 46-47 22.4487219117433 27.772794850029687 27.14965629247278 22.628826945754234 48-49 22.413800999337592 27.620611542267397 27.254585749216968 22.711001709178042 50-51 22.538410505468708 27.63994326178028 27.29988687686589 22.521759355885116 52-53 22.658521997021168 27.8060696962796 27.051725785215258 22.48368252148397 54-55 22.52184565636262 27.760173878649073 27.026568119728545 22.691412345259764 56-57 22.511288383940496 27.968169407219985 27.03923731680299 22.481304892036526 58-59 22.6314295283399 27.475064236410923 26.99240806736058 22.9010981678886 60-61 22.477986137620864 27.595336158932255 27.360502000130193 22.566175703316695 62-63 22.491817798693887 27.675538533822134 27.237731685941498 22.594911981542477 64-65 22.37576821601199 27.60833681376491 27.20286789742577 22.81302707279733 66-67 22.394716885619182 27.80011831382202 26.985132942812694 22.820031857746105 68-69 22.395099312214292 27.601066302489997 27.14929559317123 22.854538792124483 70-71 22.300671344181254 27.5757030211551 27.272676731678658 22.850948902984985 72-73 22.53801291193324 27.670826786147494 26.78227313061088 23.008887171308384 74-75 22.312040698188614 27.889503221253324 26.865770948609203 22.93268513194886 76-77 22.527899374047422 27.59724699250561 27.025238082234743 22.849615551212228 78-79 22.558541162903683 27.727490737465676 26.94771592289215 22.76625217673849 80-81 22.322792407655147 27.893232241625753 27.0554314441875 22.728543906531602 82-83 22.50958543680873 27.97478904188808 26.86766373090518 22.647961790398014 84-85 22.477896394808294 27.737530767132622 26.878716502826027 22.90585633523305 86-87 22.355293894036897 27.620015176048213 27.18812427609939 22.836566653815495 88-89 22.55417244175651 27.64802249038339 27.080201808926606 22.717603258933494 90-91 22.462937614171775 27.748373179997678 27.08101310123653 22.707676104594018 92-93 22.395846961791474 27.642209144717217 27.123978846888523 22.837965046602786 94-95 22.34281043490437 27.673260905160774 27.05453475965045 22.92939390028441 96-97 22.49142956212259 27.696449483483914 26.913366611929263 22.89875434246423 98-99 22.309492708339636 27.59634511749297 26.89116324589076 23.202998928276635 100-101 22.12014526018261 27.78239162593917 27.022750005028083 23.074713108850133 102-103 22.212565568867124 27.443336410266976 27.19978263533069 23.144315385535204 104-105 22.040196459632348 27.487507075862734 27.311996248443798 23.16030021606112 106-107 22.06506785866769 27.62593511397643 27.167292051860287 23.141704975495596 108-109 22.098029611834267 27.68831610596284 26.886325520848235 23.32732876135465 110-111 21.963567552048573 27.80044033114105 27.07730777367908 23.158684343131295 112-113 22.021656341472784 27.728879672187695 27.183474022444322 23.0659899638952 114-115 21.937993316351204 27.797956455253196 26.917020679030205 23.347029549365395 116-117 21.66704895034928 27.835440035221325 27.178277218829805 23.31923379559959 118-119 21.688122395812474 27.842304518085044 27.04222815065111 23.427344935451373 120-121 21.774757571631785 27.952180045140736 27.070940320836247 23.202122062391236 122-123 21.748900881599962 27.909261074932974 26.931919974403634 23.409918069063433 124-125 21.780926168582596 27.738578205095717 26.973640219693447 23.50685540662824 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6219.0 1 4497.5 2 2195.5 3 1396.0 4 1107.0 5 1019.0 6 1024.5 7 1161.0 8 1458.0 9 1649.5 10 1616.5 11 1625.0 12 1763.0 13 1947.5 14 2079.5 15 2220.5 16 2391.5 17 2633.0 18 2979.0 19 3324.5 20 3909.5 21 4812.0 22 5989.0 23 7503.0 24 9268.5 25 11858.5 26 15574.5 27 20346.0 28 24911.5 29 30128.5 30 37214.5 31 42947.5 32 48762.0 33 55909.0 34 63349.5 35 69474.0 36 75939.5 37 83353.5 38 89080.0 39 92729.5 40 95127.5 41 98840.5 42 102656.5 43 108006.0 44 113978.5 45 116420.5 46 116294.0 47 116510.0 48 117832.5 49 115383.0 50 111290.0 51 107391.0 52 102499.0 53 98994.0 54 95870.5 55 89971.5 56 81151.5 57 73207.5 58 67410.5 59 61197.5 60 52216.5 61 42500.0 62 33903.5 63 25987.5 64 19910.5 65 15756.5 66 11844.5 67 8838.5 68 6619.5 69 4888.0 70 3699.5 71 2736.0 72 2071.0 73 1727.5 74 1333.5 75 976.0 76 711.0 77 491.0 78 374.0 79 268.0 80 192.5 81 147.0 82 114.0 83 89.0 84 73.5 85 54.0 86 39.5 87 35.5 88 25.0 89 21.0 90 16.0 91 9.5 92 7.0 93 6.5 94 6.5 95 3.0 96 2.5 97 3.5 98 2.0 99 1.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21590499004733843 2 0.2527437177395783 3 0.30852434442250887 4 0.29435057862602654 5 0.3298829684568394 6 0.23827602592427485 7 0.2289030517685365 8 0.20790367064609838 9 0.20698923414309953 10-11 0.14810605503927995 12-13 0.1740695200351405 14-15 0.1906600108752627 16-17 0.1640433769486887 18-19 0.18997418349801357 20-21 0.18510807496419818 22-23 0.20774037841342 24-25 0.19818778280173546 26-27 0.24918394706718985 28-29 0.17725371857236866 30-31 0.1564176296826089 32-33 0.19745296775468282 34-35 0.1551276210444498 36-37 0.15453976900680766 38-39 0.18803100592914096 40-41 0.20845886423720483 42-43 0.2752453874026574 44-45 0.1743471168306937 46-47 0.15969980355944408 48-49 0.162312479282298 50-51 0.16815834121218357 52-53 0.18821062738508718 54-55 0.17571877158519203 56-57 0.17351432644403406 58-59 0.14823668882542265 60-61 0.16146335967237047 62-63 0.15011454950122388 64-65 0.14448096747382017 66-67 0.13148290575262206 68-69 0.13515698098788537 70-71 0.1363326850631696 72-73 0.1119858131708249 74-75 0.11366772316741208 76-77 0.13636534350970528 78-79 0.14309298349605404 80-81 0.15365799095034446 82-83 0.15522559638405678 84-85 0.14203158398364465 86-87 0.16997088499491345 88-89 0.16472920432593782 90-91 0.1518617763908824 92-93 0.15241696998198886 94-95 0.17346533877423054 96-97 0.16247577151497636 98-99 0.15576446075189543 100-101 0.13623470972356258 102-103 0.17571877158519203 104-105 0.13272392672097766 106-107 0.13976182194941533 108-109 0.15031050018043793 110-111 0.1254737515900581 112-113 0.11552925461994548 114-115 0.1138473446233583 116-117 0.11823990568240642 118-119 0.10122485503732044 120-121 0.09456253194404303 122-123 0.12242018683897263 124-125 0.11895839150619122 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3061995.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.919380801377606 #Duplication Level Percentage of deduplicated Percentage of total 1 78.6816070489663 43.99826746635503 2 10.297624011892669 11.516735169408717 3 4.13694859621561 6.940068117225155 4 2.1391643160912985 4.784829759529113 5 1.2255029630584922 3.4264683432242204 6 0.802168932513592 2.6914074002557276 7 0.5282551657020397 2.0677791239831023 8 0.39927341097285063 1.786169752964462 9 0.26593331024935146 1.3383743439243942 >10 1.3988969681523296 13.69019851946355 >50 0.08595186236449635 3.279282797862689 >100 0.03727814970731209 3.5991403063274703 >500 0.0010312821710453325 0.38366620557889 >1k 3.6398194258631915E-4 0.4976126938975478 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4659 0.15215570240970347 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3084 0.1007186491160175 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.2658446535673636E-5 0.0 0.0 0.0 0.0 6 3.2658446535673636E-5 0.0 0.0 0.0 0.0 7 3.2658446535673636E-5 0.0 0.0 0.0 0.0 8 3.2658446535673636E-5 0.0 0.0 0.0 0.0 9 3.2658446535673636E-5 0.0 0.0 0.0 0.0 10-11 6.531689307134727E-5 0.0 0.0 0.0 0.0 12-13 6.531689307134727E-5 0.0 0.0 3.2658446535673636E-5 0.0 14-15 6.531689307134727E-5 0.0 0.0 3.2658446535673636E-5 0.0 16-17 6.531689307134727E-5 0.0 0.0 3.2658446535673636E-5 0.0 18-19 6.531689307134727E-5 0.0 0.0 6.531689307134727E-5 0.0 20-21 9.797533960702091E-5 0.0 0.0 6.531689307134727E-5 0.0 22-23 9.797533960702091E-5 0.0 0.0 1.4696300941053136E-4 0.0 24-25 9.797533960702091E-5 0.0 0.0 3.5924291189241E-4 0.0 26-27 9.797533960702091E-5 0.0 0.0 5.062059213029414E-4 0.0 28-29 9.797533960702091E-5 0.0 0.0 0.0010450702891415563 0.0 30-31 9.797533960702091E-5 0.0 0.0 0.0031841985372281795 3.2658446535673636E-5 32-33 9.797533960702091E-5 0.0 0.0 0.007201187461116037 3.2658446535673636E-5 34-35 9.797533960702091E-5 0.0 0.0 0.012099954441467083 3.2658446535673636E-5 36-37 9.797533960702091E-5 0.0 0.0 0.021440270150669745 3.2658446535673636E-5 38-39 9.797533960702091E-5 0.0 0.0 0.03656113089668664 3.2658446535673636E-5 40-41 9.797533960702091E-5 0.0 0.0 0.05584594357600192 3.2658446535673636E-5 42-43 9.797533960702091E-5 0.0 0.0 0.0738897352869616 4.898766980351045E-5 44-45 9.797533960702091E-5 0.0 0.0 0.0946441780603822 6.531689307134727E-5 46-47 9.797533960702091E-5 0.0 0.0 0.11921965907847662 8.164611633918409E-5 48-49 9.797533960702091E-5 0.0 0.0 0.14662009572190682 9.797533960702091E-5 50-51 9.797533960702091E-5 0.0 0.0 0.17880499478281317 9.797533960702091E-5 52-53 9.797533960702091E-5 0.0 0.0 0.21157774588136166 9.797533960702091E-5 54-55 9.797533960702091E-5 0.0 0.0 0.2442361924170353 9.797533960702091E-5 56-57 9.797533960702091E-5 0.0 0.0 0.28721470805798177 1.1430456287485772E-4 58-59 1.1430456287485772E-4 0.0 0.0 0.33376932359458455 1.3063378614269454E-4 60-61 1.3063378614269454E-4 0.0 0.0 0.3873455051363572 1.3063378614269454E-4 62-63 1.4696300941053136E-4 0.0 0.0 0.4431424610425556 1.3063378614269454E-4 64-65 1.6329223267836819E-4 0.0 0.0 0.5004253762661272 1.3063378614269454E-4 66-67 1.6329223267836819E-4 0.0 0.0 0.5647461867181364 1.3063378614269454E-4 68-69 1.6329223267836819E-4 0.0 0.0 0.6447920391770725 1.3063378614269454E-4 70-71 1.6329223267836819E-4 0.0 0.0 0.7316961654085 1.3063378614269454E-4 72-73 1.6329223267836819E-4 0.0 0.0 0.8252299562866694 1.3063378614269454E-4 74-75 1.6329223267836819E-4 0.0 0.0 0.9170981663915192 1.3063378614269454E-4 76-77 1.6329223267836819E-4 0.0 0.0 1.0228462162740304 1.3063378614269454E-4 78-79 1.9595067921404183E-4 0.0 0.0 1.1464094487417515 1.3063378614269454E-4 80-81 1.9595067921404183E-4 0.0 0.0 1.292588655435427 1.79621455946205E-4 82-83 1.9595067921404183E-4 0.0 0.0 1.451586302394354 1.9595067921404183E-4 84-85 1.9595067921404183E-4 0.0 0.0 1.6097511589666214 1.9595067921404183E-4 86-87 1.9595067921404183E-4 0.0 0.0 1.7782850723139652 1.9595067921404183E-4 88-89 1.9595067921404183E-4 0.0 0.0 1.9682429265887111 1.9595067921404183E-4 90-91 1.9595067921404183E-4 0.0 0.0 2.1888670621604542 1.9595067921404183E-4 92-93 1.9595067921404183E-4 0.0 0.0 2.432629707102722 1.9595067921404183E-4 94-95 1.9595067921404183E-4 1.6329223267836818E-5 0.0 2.6779109698088988 1.9595067921404183E-4 96-97 2.612675722853891E-4 3.2658446535673636E-5 0.0 2.922196149895738 1.9595067921404183E-4 98-99 2.612675722853891E-4 3.2658446535673636E-5 0.0 3.192477453424973 1.9595067921404183E-4 100-101 2.612675722853891E-4 3.2658446535673636E-5 0.0 3.5130201061726094 1.9595067921404183E-4 102-103 2.612675722853891E-4 3.2658446535673636E-5 0.0 3.864327015556851 1.9595067921404183E-4 104-105 2.612675722853891E-4 3.2658446535673636E-5 0.0 4.217969003868393 1.9595067921404183E-4 106-107 2.9392601882106273E-4 3.2658446535673636E-5 0.0 4.5713823830541855 1.9595067921404183E-4 108-109 3.429136886245732E-4 3.2658446535673636E-5 0.0 4.946399324623325 1.9595067921404183E-4 110-111 3.9190135842808365E-4 3.2658446535673636E-5 0.0 5.379564630249233 1.9595067921404183E-4 112-113 3.9190135842808365E-4 3.2658446535673636E-5 0.0 5.857814921317638 1.9595067921404183E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11395 0.0 42.71335 1 TCAACGC 13340 0.0 35.05659 4 ATCAACG 13475 0.0 34.970642 3 CAACGCA 13615 0.0 34.305317 5 GTACATG 16505 0.0 34.289722 1 TATCAAC 14265 0.0 33.743916 2 AACGCAG 13795 0.0 32.96373 6 TACATGG 17100 0.0 32.539185 2 ACATGGG 17375 0.0 29.932661 3 ACGCAGA 15765 0.0 28.008465 7 GGTATCA 7560 0.0 27.344095 1 CGCAGAG 15465 0.0 26.431957 8 CATGGGA 11465 0.0 25.409184 4 CATGGGG 6255 0.0 25.239134 4 CGCAGAT 1085 0.0 24.677734 8 ACGCATA 340 1.8189894E-12 24.503521 7 GCAGAGT 17000 0.0 22.715292 9 CGCATAG 370 5.456968E-12 22.5138 8 GAGTACT 5285 0.0 22.177277 12-13 GTTATCA 2260 0.0 21.878904 1 >>END_MODULE