FastQCFastQC Report
Thu 26 May 2016
SRR1364614_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364614_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3061995
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119880.3915094570696556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96940.31659098071682024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63500.2073811355015276No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103400.044.816241
GTATCAA160250.044.4910281
ATCAACG201650.035.230693
TCAACGC202650.035.030344
TATCAAC203200.034.979662
CAACGCA205650.034.001775
AACGCAG211650.033.2542956
GTACATG168600.032.466411
TACATGG172950.030.909412
ACGCAGA245200.028.3885087
CGCAGAG245950.028.229318
ACATGGG178500.028.166543
GAGTACT140950.025.60178612-13
GCAGAGT265850.025.109139
CATGGGA114850.024.7137054
AGTACTT143150.024.35556812-13
GTACTTT147050.024.33728814-15
TAGACCG2701.6934791E-924.2656025
CATGGGG67650.023.4851824
CAGAGTA260150.021.42323510-11
Produced by FastQC (version 0.11.5)