##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364614_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3061995 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.596329517193855 31.0 30.0 33.0 26.0 34.0 2 29.63476818218188 31.0 30.0 34.0 26.0 34.0 3 29.652905050465463 31.0 30.0 34.0 25.0 34.0 4 33.31230880520706 35.0 33.0 37.0 28.0 37.0 5 32.72759132526343 35.0 33.0 37.0 26.0 37.0 6 32.73529316670994 35.0 33.0 37.0 25.0 37.0 7 32.515330364680544 35.0 33.0 37.0 25.0 37.0 8 32.435838072890384 35.0 33.0 37.0 23.0 37.0 9 33.68410888979244 37.0 33.0 39.0 23.0 39.0 10-11 33.48250062459279 37.0 33.0 39.0 20.5 39.0 12-13 33.34649452399498 37.0 33.0 39.0 18.5 39.0 14-15 34.29564809870689 38.0 33.0 41.0 18.5 41.0 16-17 34.2147420227662 38.0 33.0 41.0 18.5 41.0 18-19 34.27475877654928 38.5 33.0 41.0 18.0 41.0 20-21 34.30255209430453 39.0 33.0 41.0 18.0 41.0 22-23 34.30039745329434 39.0 33.0 41.0 19.0 41.0 24-25 34.30113651393944 39.0 33.0 41.0 19.0 41.0 26-27 34.19659862279331 39.0 33.0 41.0 18.5 41.0 28-29 33.95034250545804 38.0 33.0 41.0 18.0 41.0 30-31 33.874053354104106 38.0 33.0 40.5 17.5 41.0 32-33 33.675238039252186 38.0 33.0 40.0 16.5 41.0 34-35 33.53394192348452 38.0 32.5 40.0 16.0 41.0 36-37 33.35590521865646 38.0 32.0 40.0 15.0 41.0 38-39 33.20643893931897 38.0 32.0 40.0 11.0 41.0 40-41 33.01710437149636 38.0 32.0 40.0 9.0 41.0 42-43 32.821712151718074 38.0 31.5 40.0 8.0 41.0 44-45 32.59162702747719 38.0 31.0 40.0 4.5 41.0 46-47 32.289218467045174 38.0 31.0 40.0 2.0 41.0 48-49 32.00222926556052 38.0 30.0 40.0 2.0 41.0 50-51 31.78703851573892 37.5 30.0 40.0 2.0 41.0 52-53 31.616449732935553 37.0 30.0 40.0 2.0 41.0 54-55 31.405775319685368 37.0 30.0 40.0 2.0 41.0 56-57 31.177744901608264 37.0 29.0 40.0 2.0 41.0 58-59 30.993103679137292 36.0 29.0 40.0 2.0 41.0 60-61 30.77564724958728 36.0 29.0 40.0 2.0 41.0 62-63 30.516215571873893 35.5 28.5 39.5 2.0 41.0 64-65 30.25339378411787 35.0 28.0 39.0 2.0 41.0 66-67 29.979226941912053 35.0 28.0 39.0 2.0 41.0 68-69 29.674560703071037 35.0 28.0 38.5 2.0 40.0 70-71 29.42234409265854 35.0 28.0 38.0 2.0 40.0 72-73 29.267309058310023 35.0 28.0 37.0 2.0 40.0 74-75 28.980434814557178 34.5 28.0 37.0 2.0 39.0 76-77 28.682668162423518 34.0 28.0 36.0 2.0 39.0 78-79 28.375433010178007 34.0 27.0 36.0 2.0 39.0 80-81 28.10191247862913 34.0 26.5 35.5 2.0 37.0 82-83 27.84606784139099 34.0 26.0 35.0 2.0 37.0 84-85 27.82386434334478 34.0 27.0 35.0 2.0 36.5 86-87 27.67406821369728 34.0 27.0 35.0 2.0 36.0 88-89 27.533365012026472 34.0 27.0 35.0 2.0 36.0 90-91 27.385668167322287 34.0 26.0 35.0 2.0 36.0 92-93 27.250284373423206 34.0 26.0 35.0 2.0 35.0 94-95 27.16901219629686 34.0 26.0 35.0 2.0 35.0 96-97 27.086547169410792 34.0 26.0 35.0 2.0 35.0 98-99 26.99799232199922 34.0 26.0 35.0 2.0 35.0 100-101 26.901090465529826 34.0 25.0 35.0 2.0 35.0 102-103 25.733345090374087 31.5 23.5 34.0 2.0 35.0 104-105 26.65281687265982 33.5 25.0 35.0 2.0 35.0 106-107 26.8274907372481 34.0 25.0 35.0 2.0 35.0 108-109 26.83584117544281 34.0 25.0 35.0 2.0 35.0 110-111 26.77905532177551 34.0 25.0 35.0 2.0 35.0 112-113 26.729317487455077 34.0 25.0 35.0 2.0 35.0 114-115 26.644640340692916 34.0 25.0 35.0 2.0 35.0 116-117 26.534747933945027 34.0 25.0 35.0 2.0 35.0 118-119 26.432788917029583 34.0 25.0 35.0 2.0 35.0 120-121 26.29908066473002 34.0 25.0 35.0 2.0 35.0 122-123 26.115136210215887 34.0 24.0 35.0 2.0 35.0 124-125 25.308546715458384 33.0 21.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6685986630246035 1101 2 0.6569482803684572 1101 3 0.5073404165588897 1101 4 0.7047735095915257 1101 5 0.9425853612054453 1101 6 1.0350059829341163 1101 7 0.8633671348017842 1101 8 1.0483260123574638 1101 9 1.170940539012122 1101 10-11 1.158728954978173 1101 12-13 1.130425820768771 1101 14-15 1.1642034557667742 1101 16-17 1.3775449183676827 1101 18-19 1.2903384001947487 1101 20-21 1.2167397355649143 1101 22-23 1.2132453884767713 1101 24-25 1.0417514293300414 1101 26-27 1.1409026337915336 1101 28-29 1.277154927867258 1101 30-31 1.1658279526841326 1101 32-33 1.2248817587727316 1101 34-35 1.227581793823397 1101 36-37 1.2604855525444236 1101 38-39 1.2372508964333662 1101 40-41 1.3724846909923443 1101 42-43 1.2939885703294536 1101 44-45 1.2119594564385139 1101 46-47 1.25551322691161 1101 48-49 1.2703747290296263 1101 50-51 1.242712753613013 1101 52-53 1.2960578906874538 1101 54-55 1.3207235592340858 1101 56-57 1.2951603689274975 1101 58-59 1.2938863050825447 1101 60-61 1.2582496774401548 1101 62-63 1.2475029597709728 1101 64-65 1.2111478295130915 1101 66-67 1.2187388261300036 1101 68-69 1.239111112418236 1101 70-71 1.2108240995691233 1101 72-73 1.230221243379379 1101 74-75 1.1722697007587364 1101 76-77 1.1628651499187654 1101 78-79 1.124454979187174 1101 80-81 1.14401183104928 1101 82-83 1.1033231247767006 1101 84-85 1.173370676629446 1101 86-87 1.1195483861654516 1101 88-89 1.0476725896954733 1101 90-91 1.0575314373742941 1101 92-93 1.1299091550993055 1101 94-95 1.0475588906768785 1101 96-97 1.0901187995784376 1101 98-99 1.0804726522996226 1101 100-101 1.0872811206240272 1101 102-103 1.1112614513568069 1101 104-105 1.060700912295296 1101 106-107 0.9628605187043568 1101 108-109 0.9501328076575568 1101 110-111 1.049088480082176 1101 112-113 1.0724809686629655 1101 114-115 0.9683661274810582 1101 116-117 1.0019034286896868 1101 118-119 1.0031220264236929 1101 120-121 1.0407921298660305 1101 122-123 1.1296876855786877 1101 124-125 0.9623481384179087 1102 1 0.4672881715398809 1102 2 -0.49071271765626534 1102 3 0.07725368875133753 1102 4 0.4124524851347502 1102 5 0.8381291142407647 1102 6 0.7525326963999888 1102 7 0.6692309258669908 1102 8 0.5952618111310386 1102 9 0.9144347899777756 1102 10-11 0.9868727446821239 1102 12-13 1.0584424967544948 1102 14-15 1.1611334032662555 1102 16-17 1.2442896165655597 1102 18-19 1.2593205877917342 1102 20-21 1.198400184626692 1102 22-23 1.2285708060914615 1102 24-25 1.2524870774926882 1102 26-27 1.164514900156263 1102 28-29 1.2676719856158911 1102 30-31 1.2011618190150415 1102 32-33 1.084475710407176 1102 34-35 0.9903646219676503 1102 36-37 0.9094137368082613 1102 38-39 0.8131828609014491 1102 40-41 0.8170582426711164 1102 42-43 0.6031000366645571 1102 44-45 0.5124942250775888 1102 46-47 0.521819617656682 1102 48-49 0.5870723213200222 1102 50-51 0.4596263437230732 1102 52-53 0.5793223838861543 1102 54-55 0.5703054621886459 1102 56-57 0.4569082487253624 1102 58-59 0.4557396241608096 1102 60-61 0.33876936559585147 1102 62-63 0.4552677168345056 1102 64-65 0.5113961668931957 1102 66-67 0.5676398046356113 1102 68-69 0.3704537456738528 1102 70-71 0.2660588907891679 1102 72-73 0.44431742320032797 1102 74-75 0.48493350128681456 1102 76-77 0.4312442587663341 1102 78-79 0.4963038838742371 1102 80-81 0.3920787048584842 1102 82-83 0.29224222235607655 1102 84-85 0.4609864671385182 1102 86-87 0.4983135743908065 1102 88-89 0.5575372463808144 1102 90-91 0.5623432498314074 1102 92-93 0.6098017146540791 1102 94-95 0.3588644636009626 1102 96-97 0.37317643787622856 1102 98-99 0.4065317013992278 1102 100-101 0.32190644953398717 1102 102-103 0.3385591381014059 1102 104-105 0.32134863136975866 1102 106-107 0.49695027424326454 1102 108-109 0.5138373544805006 1102 110-111 0.39674922602218743 1102 112-113 0.34037302075180875 1102 114-115 0.5181696807702636 1102 116-117 0.49924042028935034 1102 118-119 0.4760213923627603 1102 120-121 0.5278895857204198 1102 122-123 0.41861550431969263 1102 124-125 0.3574182145756133 1103 1 0.2187227803736711 1103 2 -1.0495528529345535 1103 3 -0.007248995143530834 1103 4 0.2773547649974759 1103 5 0.4450885909272415 1103 6 0.5492922397516651 1103 7 0.4977561403846309 1103 8 0.5107156286065688 1103 9 0.641737565499291 1103 10-11 0.849544285353069 1103 12-13 0.9210611476035524 1103 14-15 0.8310377999478646 1103 16-17 1.0542634992429356 1103 18-19 0.9765985229966248 1103 20-21 0.977967617972638 1103 22-23 0.8751624628777606 1103 24-25 0.7588312569910869 1103 26-27 0.8202549023248196 1103 28-29 0.960286001678611 1103 30-31 0.9527758658259415 1103 32-33 0.9084644396032928 1103 34-35 0.9733540096261137 1103 36-37 0.962620701473405 1103 38-39 0.9369214558133265 1103 40-41 1.0220894010778707 1103 42-43 1.061301948475915 1103 44-45 0.9459848939840185 1103 46-47 0.9651160154061884 1103 48-49 0.9609650372219392 1103 50-51 1.002622679392637 1103 52-53 1.0985828365651606 1103 54-55 1.0444693849352795 1103 56-57 0.9543404074142501 1103 58-59 0.9741118367850525 1103 60-61 0.9140357260458956 1103 62-63 0.9303842693773454 1103 64-65 0.9692647281581195 1103 66-67 0.8363900311398886 1103 68-69 0.7826058357143459 1103 70-71 0.8696854750134264 1103 72-73 0.9019956105305376 1103 74-75 0.8560019938758892 1103 76-77 0.8765352427754607 1103 78-79 0.8571143256246394 1103 80-81 0.9691830908297199 1103 82-83 0.9434154814190556 1103 84-85 0.9403240050778301 1103 86-87 0.9195359133449763 1103 88-89 0.927845199514298 1103 90-91 0.8576317597640291 1103 92-93 0.9491081405295922 1103 94-95 0.85808320467412 1103 96-97 0.8473075527500313 1103 98-99 0.9052849041513475 1103 100-101 0.8545630089238117 1103 102-103 0.8946881366957662 1103 104-105 0.8996715674517368 1103 106-107 0.8791910490151587 1103 108-109 0.8088410145934901 1103 110-111 0.8757179328530427 1103 112-113 0.8822936358018367 1103 114-115 0.7866176236749531 1103 116-117 0.8219307865002428 1103 118-119 0.8371885522999634 1103 120-121 0.7971878361094191 1103 122-123 0.7903709025511176 1103 124-125 0.7043557075352851 1104 1 0.38729352646765136 1104 2 -0.6707611947682395 1104 3 0.16363656179684583 1104 4 0.32826160282557026 1104 5 0.7377615138548848 1104 6 0.6580462049170706 1104 7 0.7971266284107017 1104 8 0.8115044615121718 1104 9 0.8928926136813047 1104 10-11 0.969615436892532 1104 12-13 0.8819929464728773 1104 14-15 0.9158657670573476 1104 16-17 1.0555590532860464 1104 18-19 0.9521016439839656 1104 20-21 0.994698030155547 1104 22-23 0.9805708599137475 1104 24-25 1.0444128126788357 1104 26-27 0.8322214729303425 1104 28-29 1.0262266094868124 1104 30-31 0.9227892578131573 1104 32-33 1.0774546317655975 1104 34-35 0.9816758567660244 1104 36-37 1.0229859338516718 1104 38-39 0.9990337610694837 1104 40-41 1.0125106443640206 1104 42-43 1.0104492557635893 1104 44-45 0.9283897314429552 1104 46-47 0.9895869647657989 1104 48-49 0.9822447106026004 1104 50-51 0.9561027388465462 1104 52-53 0.7836872009186528 1104 54-55 0.916734699431256 1104 56-57 1.0007519148657025 1104 58-59 0.988365145637367 1104 60-61 1.0458621536625898 1104 62-63 1.014017329499282 1104 64-65 1.084065374127924 1104 66-67 1.028773627712777 1104 68-69 0.9444308004128743 1104 70-71 0.9813517045189997 1104 72-73 0.97998422091257 1104 74-75 1.0188540728483275 1104 76-77 0.904694488846868 1104 78-79 0.8436674789847523 1104 80-81 0.7608459716241747 1104 82-83 0.852115887207411 1104 84-85 0.8829210391255344 1104 86-87 0.8240544304031161 1104 88-89 0.835520205385972 1104 90-91 0.9154169559464975 1104 92-93 0.891333873374446 1104 94-95 0.9051028803520218 1104 96-97 0.9211017738803982 1104 98-99 0.922668272143266 1104 100-101 0.9747590942462274 1104 102-103 0.9597284926649046 1104 104-105 0.9451130344386449 1104 106-107 0.9268409652688092 1104 108-109 0.8735583570883456 1104 110-111 0.8571850169085522 1104 112-113 0.8729283879943566 1104 114-115 0.9361041451127043 1104 116-117 0.9436075764463396 1104 118-119 0.9206471051424536 1104 120-121 0.9799363879061538 1104 122-123 0.9234098807484941 1104 124-125 0.8871126882550513 1105 1 0.44353938649729585 1105 2 0.2457025576563332 1105 3 0.5005124473420715 1105 4 0.43250401424134566 1105 5 0.511324257240581 1105 6 0.47186171298611157 1105 7 0.5061140028006577 1105 8 0.5898272425623219 1105 9 0.5551944891806997 1105 10-11 0.6560620650630469 1105 12-13 0.6306361315247173 1105 14-15 0.7744666575779675 1105 16-17 0.721294133798267 1105 18-19 0.8137482902738071 1105 20-21 0.9399056153238377 1105 22-23 0.7188206772352359 1105 24-25 0.675987011343949 1105 26-27 0.7550000725138943 1105 28-29 0.9110942195469107 1105 30-31 0.7824955946782737 1105 32-33 0.8410290004850864 1105 34-35 0.83725726083604 1105 36-37 0.8042075295707534 1105 38-39 0.9108597256268922 1105 40-41 0.8188213886824158 1105 42-43 0.7931819406624641 1105 44-45 0.9471089879859633 1105 46-47 0.9296649781443946 1105 48-49 0.9789531556965656 1105 50-51 1.0644600580170973 1105 52-53 0.9658406055708646 1105 54-55 0.9422725614094638 1105 56-57 0.9511452306143724 1105 58-59 0.882546110981913 1105 60-61 1.0131622357936614 1105 62-63 0.983790347734459 1105 64-65 0.9417257673471333 1105 66-67 0.8528560766147741 1105 68-69 0.8197847400309435 1105 70-71 0.9419972948379289 1105 72-73 0.8665176585063215 1105 74-75 0.8888495355893369 1105 76-77 0.894097557103283 1105 78-79 0.9091668225302598 1105 80-81 0.7416670484174723 1105 82-83 0.77149553399925 1105 84-85 0.6921406231531968 1105 86-87 0.7589792945823852 1105 88-89 0.9111575566069483 1105 90-91 0.7783443011375972 1105 92-93 0.6189931638392245 1105 94-95 0.725689931861421 1105 96-97 0.7997616561938514 1105 98-99 0.8774153329499619 1105 100-101 0.8796477309001496 1105 102-103 0.44493171879713955 1105 104-105 0.3714120940156036 1105 106-107 0.7579349088077798 1105 108-109 0.7675454121622067 1105 110-111 0.6236670186194928 1105 112-113 0.5132420083854363 1105 114-115 0.6956585654871503 1105 116-117 0.4174343311254347 1105 118-119 0.3088339317939095 1105 120-121 0.28319283660616534 1105 122-123 0.3176921175651941 1105 124-125 0.1977168417406432 1106 1 0.3244861462694395 1106 2 0.27646732006560626 1106 3 0.4885523499977502 1106 4 0.3765289092865842 1106 5 0.4609713678362368 1106 6 0.528273903904541 1106 7 0.5498858841629897 1106 8 0.6095782506115555 1106 9 0.6792866223911531 1106 10-11 0.8326902170437407 1106 12-13 0.7109220344604239 1106 14-15 0.8434129312780456 1106 16-17 1.0302250494974174 1106 18-19 0.9012202427967964 1106 20-21 0.9541772614085176 1106 22-23 0.7630145562996447 1106 24-25 0.8818762417623134 1106 26-27 0.8259779614671743 1106 28-29 0.9809589601605211 1106 30-31 0.9063213490282038 1106 32-33 0.7993123027367588 1106 34-35 0.7059774090826139 1106 36-37 0.7907746239351354 1106 38-39 0.9090469916573412 1106 40-41 0.8369564191061301 1106 42-43 0.8829380620310872 1106 44-45 0.8398174423561713 1106 46-47 0.702664924196533 1106 48-49 0.8091963649413927 1106 50-51 0.8431347015101167 1106 52-53 0.8836195710637149 1106 54-55 0.8560693760676976 1106 56-57 0.769978872358692 1106 58-59 0.7196207392829308 1106 60-61 0.8379440130708922 1106 62-63 0.8054530498043988 1106 64-65 0.7303590243475462 1106 66-67 0.7332651169057094 1106 68-69 0.7651454536800024 1106 70-71 0.7441666395319508 1106 72-73 0.8079987944354556 1106 74-75 0.8687985665846405 1106 76-77 0.862509751449835 1106 78-79 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-1.80856765091918 2216 108-109 -1.8974143148002511 2216 110-111 -1.7780147219948432 2216 112-113 -1.8475166946862345 2216 114-115 -1.8780619123285511 2216 116-117 -1.9652682286015306 2216 118-119 -2.00561111940063 2216 120-121 -1.9676697913801853 2216 122-123 -1.9614126605918045 2216 124-125 -2.0125930558702763 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 122817.0 3 38524.0 4 17704.0 5 14011.0 6 15193.0 7 18399.0 8 20412.0 9 21751.0 10 23716.0 11 26250.0 12 30067.0 13 33905.0 14 37760.0 15 30641.0 16 19897.0 17 17127.0 18 15417.0 19 14525.0 20 13831.0 21 14316.0 22 17066.0 23 22453.0 24 30886.0 25 41200.0 26 49050.0 27 54113.0 28 61955.0 29 72225.0 30 84302.0 31 99153.0 32 119928.0 33 148406.0 34 190371.0 35 258473.0 36 404667.0 37 617303.0 38 243325.0 39 856.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6863241743752 19.720032142351794 13.8343806175998 23.759263065673203 2 15.556250620983219 20.932461865093682 38.98065277755989 24.530634736363208 3 19.07272872751262 25.91522847032735 29.39263454055281 25.61940826160722 4 12.784412323401764 17.486328089276988 36.432571939203434 33.29668764811781 5 12.999336178531387 39.25386728274874 33.48286739512797 14.263929143591902 6 31.307154471916437 37.51443098876644 17.10023589257564 14.07817864674148 7 27.844978040439035 33.80942863338637 20.435520406323725 17.91007291985087 8 26.4112776147577 35.96050940644906 18.967666505007355 18.66054647378588 9 26.72782555021767 16.112372100314502 19.75375330261237 37.40604904685546 10-11 24.693430516200106 27.68705191019953 26.624038053994138 20.99547951960623 12-13 25.715505873034665 24.834040329396203 27.771790652954074 21.678663144615058 14-15 22.762750061239487 25.78162815383359 26.299893851555485 25.155727933371434 16-17 21.747408072299827 29.169475425659737 26.840212301335104 22.24290420070534 18-19 21.68111615013703 27.19034470888943 29.788655382391088 21.339883758582452 20-21 22.629525781856422 26.253009212874346 29.931152900267975 21.18631210500126 22-23 22.285308520961813 26.060861819883847 29.86120615065827 21.792623508496074 24-25 21.886400859570312 26.323132467557915 30.133638363223973 21.656828309647793 26-27 21.810127057686245 26.5666338449279 29.96325924764737 21.659979849738487 28-29 21.624015366917504 26.40008521927544 30.136074727632433 21.839824686174627 30-31 21.753464652946853 26.289853510538062 30.232560797780533 21.724121038734552 32-33 21.67160210211338 26.33265738497801 29.98676299556709 22.00897751734152 34-35 21.98034288103018 26.299079521684394 29.97222399122141 21.74835360606402 36-37 22.109158624928497 26.100937183570117 29.851353374852035 21.93855081664935 38-39 21.986564315095226 26.339575995388625 29.81786384367055 21.855995845845598 40-41 22.033200576748165 26.289379963063297 29.765626658436737 21.9117928017518 42-43 21.948247079281963 26.317529893034514 29.525351373692367 22.208871653991157 44-45 22.110601095037712 26.456003357288303 29.205664934136077 22.227730613537904 46-47 22.267466885957322 26.78880167629329 28.59959882351174 22.34413261423765 48-49 22.357177941679794 26.795416186587033 28.378944164452424 22.468461707280753 50-51 22.435765859460613 26.941851216279396 28.238870809637483 22.38351211462251 52-53 22.557124197478732 27.06028970370744 28.025135936901176 22.357450161912652 54-55 22.34576188820718 27.134944233244052 27.98403132337436 22.535262555174413 56-57 22.401817669071946 27.26495443171959 27.987077488529756 22.346150410678707 58-59 22.490941363392167 26.80086022348175 28.001058133667755 22.707140279458326 60-61 22.380588472548126 26.934972134180494 28.31572226603897 22.36871712723241 62-63 22.36938662538639 26.982963721364666 28.299703298013224 22.34794635523572 64-65 22.26872993024932 26.833900641007325 28.330384530911807 22.56698489783155 66-67 22.222978809566964 27.103163133839214 28.072923045269505 22.600935011324317 68-69 22.272431266606922 26.89248707370665 28.248016966821428 22.587064692865 70-71 22.123354218409894 26.88208178001597 28.257443268196063 22.737120733378074 72-73 22.399476277435138 27.02817980771256 27.82556994754773 22.74677396730457 74-75 22.2172234982123 27.238415457107003 27.811655699865362 22.732705344815336 76-77 22.38498004313876 27.017277548722973 27.90422012949466 22.693522278643602 78-79 22.3912366164506 27.120907207390804 27.850438018947294 22.637418157211307 80-81 22.22718094804197 27.272115658605607 27.831074554796505 22.669628838555916 82-83 22.43118205403541 27.27543050406402 27.74228345469224 22.551103987208325 84-85 22.359326099047255 27.113909143489224 27.708603514116458 22.818161243347063 86-87 22.20318045579947 26.960196539333587 27.997122779114513 22.83950022575243 88-89 22.401085305672495 26.988242467450547 27.885353811053548 22.725318415823413 90-91 22.30371048940315 27.11010305372804 27.90905928977676 22.677127167092042 92-93 22.19411763264966 27.002566319727674 27.964785161560563 22.8385308860621 94-95 22.161025757765536 26.990302645702407 27.86782151564048 22.980850080891578 96-97 22.31969523968782 26.966120759706 27.72509843831243 22.989085562293752 98-99 22.170309366293388 26.953865306931586 27.65395404068428 23.22187128609075 100-101 22.013379573550587 27.000412478065673 27.81480355364713 23.171404394736612 102-103 22.094852320785346 26.698571828171307 27.906086610625795 23.30048924041755 104-105 22.012541541693228 26.65518500423255 28.037785305213873 23.294488148860346 106-107 22.02570399224785 26.8145920501238 27.8473469875654 23.312356970062957 108-109 22.038627100044415 26.832541870247955 27.608104930368665 23.520726099338958 110-111 21.913214297669718 26.915733102980354 27.760467738472077 23.41058486087785 112-113 21.981253470067603 26.791077435579215 27.850746268656717 23.376922825696465 114-115 21.8315498503259 26.847901982569482 27.631658015418754 23.688890151685868 116-117 21.588042691423194 26.818916130089992 27.858098487994404 23.734942690492407 118-119 21.585125450369834 26.889169177067522 27.68876341964932 23.836941952913328 120-121 21.63411147232136 26.975122002364106 27.70863865906329 23.682127866251243 122-123 21.57028952659143 26.954121602997294 27.52690756651613 23.948681303895146 124-125 21.5954952735023 26.802636041633086 27.542918467200284 24.058950217664325 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 275.0 1 211.0 2 274.0 3 510.0 4 455.5 5 445.5 6 1087.0 7 2341.5 8 3803.5 9 4434.0 10 3700.0 11 2819.0 12 2728.5 13 3011.5 14 3316.5 15 3613.5 16 3904.5 17 4319.0 18 4780.0 19 5098.0 20 5501.0 21 6207.5 22 7264.0 23 8609.0 24 10196.0 25 12685.5 26 16318.5 27 20737.5 28 24873.5 29 29819.5 30 36782.0 31 41859.0 32 47198.5 33 54530.5 34 61273.5 35 66902.0 36 73768.0 37 81438.0 38 87209.0 39 91366.0 40 93715.5 41 97014.0 42 100972.5 43 106215.5 44 113060.5 45 116598.5 46 116630.5 47 116884.5 48 119443.0 49 117485.5 50 113310.5 51 109184.0 52 103492.0 53 99650.0 54 97122.5 55 92051.5 56 82336.5 57 73350.0 58 68001.0 59 62043.5 60 52403.5 61 42355.0 62 33465.0 63 25238.5 64 19216.5 65 15094.0 66 10779.5 67 8013.5 68 5916.0 69 3938.0 70 2855.0 71 2018.0 72 1571.5 73 1432.5 74 1088.0 75 809.5 76 596.0 77 344.0 78 219.5 79 153.5 80 108.5 81 76.5 82 64.0 83 47.5 84 33.0 85 24.5 86 11.0 87 10.0 88 8.0 89 4.5 90 4.5 91 1.5 92 1.0 93 1.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06067939366328162 2 0.07612683887465525 3 0.0 4 0.007936002508168694 5 0.03040501372471216 6 2.6126757228538914E-4 7 9.797533960702091E-5 8 0.0 9 8.164611633918408E-4 10-11 0.06276953424156473 12-13 0.010858933473111483 14-15 0.008001319401240041 16-17 0.05316795096007668 18-19 1.79621455946205E-4 20-21 0.011610077743431977 22-23 0.01319401240041215 24-25 0.0 26-27 0.0 28-29 0.054033399793272034 30-31 0.0 32-33 0.004049647370423531 34-35 0.0 36-37 4.898766980351046E-5 38-39 0.0 40-41 0.0 42-43 0.003902684361013 44-45 0.0 46-47 3.265844653567364E-5 48-49 9.797533960702091E-5 50-51 4.408890282315941E-4 52-53 1.4696300941053136E-4 54-55 1.632922326783682E-5 56-57 0.0016819099965871924 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.715228143742887E-4 66-67 0.0 68-69 6.205104841777991E-4 70-71 0.0 72-73 0.002237103587693644 74-75 0.003347490769906548 76-77 5.062059213029414E-4 78-79 8.817780564631882E-4 80-81 0.0017145684431228657 82-83 9.797533960702091E-5 84-85 6.531689307134728E-5 86-87 4.082305816959204E-4 88-89 1.632922326783682E-5 90-91 0.0 92-93 0.007103212121509017 94-95 0.0058948495996890915 96-97 0.005845861929885581 98-99 8.491196099275146E-4 100-101 4.572182514994309E-4 102-103 1.1430456287485772E-4 104-105 3.5924291189241E-4 106-107 0.0044415487288516145 108-109 0.005062059213029413 110-111 8.981072797310251E-4 112-113 0.0031025524208889953 114-115 2.9392601882106273E-4 116-117 0.002237103587693644 118-119 0.0021881159178901337 120-121 0.01221425900434194 122-123 0.0010124118426058828 124-125 0.0018125437827298868 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3061995.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.778927917259075 #Duplication Level Percentage of deduplicated Percentage of total 1 73.63333697463581 34.44488562643736 2 12.036691410855138 11.261270397413679 3 5.0208476584147315 7.046096120895652 4 2.6915644880712333 5.036340046885543 5 1.650151687924453 3.85961634309807 6 1.0837987703951084 3.041936673427608 7 0.7270057944678654 2.3805986158389363 8 0.5361376986753721 2.006395740804832 9 0.3876221723932659 1.6319294695364361 >10 2.0220880691533 16.879289034333716 >50 0.1375261253776624 4.44639452417014 >100 0.06950284481047547 5.726712550835563 >500 0.002882613167158998 0.8778445536460063 >1k 6.327687439077969E-4 0.43874138638943966 >5k 1.4061527642395487E-4 0.5276736734057099 >10k+ 7.030763821197744E-5 0.39427524288130456 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11988 0.3915094570696556 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9694 0.31659098071682024 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6350 0.2073811355015276 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.2658446535673636E-5 0.0 5 0.0 0.0 0.0 3.2658446535673636E-5 0.0 6 0.0 0.0 0.0 3.2658446535673636E-5 0.0 7 0.0 0.0 0.0 3.2658446535673636E-5 0.0 8 0.0 0.0 0.0 3.2658446535673636E-5 0.0 9 0.0 0.0 0.0 3.2658446535673636E-5 0.0 10-11 0.0 0.0 0.0 3.2658446535673636E-5 0.0 12-13 0.0 0.0 0.0 6.531689307134727E-5 0.0 14-15 0.0 0.0 0.0 6.531689307134727E-5 0.0 16-17 0.0 0.0 0.0 6.531689307134727E-5 0.0 18-19 0.0 0.0 0.0 6.531689307134727E-5 0.0 20-21 3.2658446535673636E-5 0.0 0.0 9.79753396070209E-5 0.0 22-23 3.2658446535673636E-5 0.0 0.0 2.2860912574971544E-4 0.0 24-25 3.2658446535673636E-5 0.0 0.0 4.5721825149943094E-4 0.0 26-27 3.2658446535673636E-5 1.6329223267836818E-5 0.0 5.878520376421255E-4 0.0 28-29 3.2658446535673636E-5 3.2658446535673636E-5 0.0 0.0011267164054807406 0.0 30-31 3.2658446535673636E-5 3.2658446535673636E-5 0.0 0.0032985031001030373 0.0 32-33 3.2658446535673636E-5 3.2658446535673636E-5 0.0 0.00744612581013359 0.0 34-35 6.531689307134727E-5 3.2658446535673636E-5 0.0 0.012687806479109208 0.0 36-37 6.531689307134727E-5 3.2658446535673636E-5 0.0 0.02242002354673995 0.0 38-39 6.531689307134727E-5 3.2658446535673636E-5 0.0 0.038455320795755706 0.0 40-41 6.531689307134727E-5 3.2658446535673636E-5 0.0 0.05870355764787336 0.0 42-43 6.531689307134727E-5 3.2658446535673636E-5 0.0 0.0774821644058857 0.0 44-45 6.531689307134727E-5 3.2658446535673636E-5 0.0 0.09892243455655544 0.0 46-47 6.531689307134727E-5 3.2658446535673636E-5 0.0 0.12339994023504283 0.0 48-49 6.531689307134727E-5 3.2658446535673636E-5 0.0 0.1509146814413479 0.0 50-51 6.531689307134727E-5 3.2658446535673636E-5 0.0 0.1840956631215923 0.0 52-53 6.531689307134727E-5 3.2658446535673636E-5 0.0 0.21745626625778292 0.0 54-55 6.531689307134727E-5 3.2658446535673636E-5 0.0 0.2508985155103127 0.0 56-57 6.531689307134727E-5 3.2658446535673636E-5 0.0 0.2943505786260265 0.0 58-59 8.164611633918409E-5 3.2658446535673636E-5 0.0 0.3417869722190925 0.0 60-61 9.797533960702091E-5 3.2658446535673636E-5 0.0 0.39528150764452585 0.0 62-63 9.797533960702091E-5 3.2658446535673636E-5 0.0 0.45204188772352666 0.0 64-65 9.797533960702091E-5 3.2658446535673636E-5 0.0 0.5107127869248643 3.2658446535673636E-5 66-67 1.3063378614269454E-4 3.2658446535673636E-5 0.0 0.5755071448516409 3.2658446535673636E-5 68-69 1.6329223267836819E-4 3.2658446535673636E-5 0.0 0.6570389566279501 3.2658446535673636E-5 70-71 1.6329223267836819E-4 3.2658446535673636E-5 0.0 0.7467517092614455 3.2658446535673636E-5 72-73 1.6329223267836819E-4 3.2658446535673636E-5 0.0 0.8411999366426137 3.2658446535673636E-5 74-75 1.6329223267836819E-4 3.2658446535673636E-5 0.0 0.9343091677158193 3.2658446535673636E-5 76-77 1.6329223267836819E-4 3.2658446535673636E-5 0.0 1.0421636873998814 3.2658446535673636E-5 78-79 1.6329223267836819E-4 3.2658446535673636E-5 0.0 1.1689437768513664 3.2658446535673636E-5 80-81 1.6329223267836819E-4 3.2658446535673636E-5 0.0 1.3171804656767891 3.2658446535673636E-5 82-83 1.6329223267836819E-4 3.2658446535673636E-5 0.0 1.4774681212738754 3.2658446535673636E-5 84-85 1.6329223267836819E-4 3.2658446535673636E-5 0.0 1.637118937163516 3.2658446535673636E-5 86-87 1.6329223267836819E-4 3.2658446535673636E-5 0.0 1.808722744485213 3.2658446535673636E-5 88-89 1.6329223267836819E-4 3.2658446535673636E-5 0.0 2.0015218836085626 3.2658446535673636E-5 90-91 1.79621455946205E-4 3.2658446535673636E-5 0.0 2.2244647688843386 3.2658446535673636E-5 92-93 2.1227990248187865E-4 3.2658446535673636E-5 0.0 2.471493258480174 3.2658446535673636E-5 94-95 2.2860912574971547E-4 3.2658446535673636E-5 0.0 2.719958719723579 3.2658446535673636E-5 96-97 2.612675722853891E-4 3.2658446535673636E-5 0.0 2.968097596501627 4.898766980351045E-5 98-99 2.612675722853891E-4 3.2658446535673636E-5 0.0 3.2431143747785347 6.531689307134727E-5 100-101 2.775967955532259E-4 3.2658446535673636E-5 0.0 3.569127317320897 6.531689307134727E-5 102-103 3.2658446535673637E-4 3.2658446535673636E-5 0.0 3.9246471663082403 6.531689307134727E-5 104-105 3.5924291189241E-4 3.2658446535673636E-5 0.0 4.282044875971384 6.531689307134727E-5 106-107 3.5924291189241E-4 3.2658446535673636E-5 0.0 4.640095754565243 6.531689307134727E-5 108-109 3.9190135842808365E-4 3.2658446535673636E-5 0.0 5.021448434762304 6.531689307134727E-5 110-111 4.408890282315941E-4 3.2658446535673636E-5 0.0 5.460753528336918 6.531689307134727E-5 112-113 4.5721825149943094E-4 3.2658446535673636E-5 0.0 5.947299064825383 6.531689307134727E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10340 0.0 44.81624 1 GTATCAA 16025 0.0 44.491028 1 ATCAACG 20165 0.0 35.23069 3 TCAACGC 20265 0.0 35.03034 4 TATCAAC 20320 0.0 34.97966 2 CAACGCA 20565 0.0 34.00177 5 AACGCAG 21165 0.0 33.254295 6 GTACATG 16860 0.0 32.46641 1 TACATGG 17295 0.0 30.90941 2 ACGCAGA 24520 0.0 28.388508 7 CGCAGAG 24595 0.0 28.22931 8 ACATGGG 17850 0.0 28.16654 3 GAGTACT 14095 0.0 25.601786 12-13 GCAGAGT 26585 0.0 25.10913 9 CATGGGA 11485 0.0 24.713705 4 AGTACTT 14315 0.0 24.355568 12-13 GTACTTT 14705 0.0 24.337288 14-15 TAGACCG 270 1.6934791E-9 24.265602 5 CATGGGG 6765 0.0 23.485182 4 CAGAGTA 26015 0.0 21.423235 10-11 >>END_MODULE