FastQCFastQC Report
Thu 26 May 2016
SRR1364613_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364613_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2750880
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55450.046.5199051
ATCAACG64250.039.5905383
TCAACGC64050.038.97014
CAACGCA64850.038.490765
TATCAAC70100.036.6265372
AACGCAG67150.036.5160836
GTACATG92950.033.263691
GGTATCA34300.032.4787981
TACATGG96700.031.4181332
ACGCAGA78050.030.4986467
ACATGGG102100.028.9396043
CGCAGAG78150.027.9431388
CGCATAG2809.458745E-1125.5012428
CATGGGG54400.024.0913094
GCAGAGT91450.021.9272569
GTGTAGC17550.020.366981
GTACCTG26700.019.6346821
TAGCCCT20900.019.382034
TACCTGG25200.019.14792
AGTACAT72100.018.7554382
Produced by FastQC (version 0.11.5)