##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364613_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2750880 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.758534723433957 30.0 26.0 31.0 2.0 34.0 2 25.434687445471994 30.0 25.0 31.0 2.0 34.0 3 25.86490214040598 31.0 25.0 31.0 2.0 34.0 4 29.015248211481417 35.0 28.0 37.0 2.0 37.0 5 29.32038329552725 35.0 28.0 37.0 2.0 37.0 6 29.41293876868493 35.0 29.0 37.0 2.0 37.0 7 29.120214622229977 35.0 28.0 37.0 2.0 37.0 8 28.9609990257663 35.0 28.0 37.0 2.0 37.0 9 29.786408349328216 35.0 27.0 39.0 2.0 39.0 10-11 29.707092094166228 35.0 27.5 39.0 2.0 39.0 12-13 29.756023708776826 35.0 27.0 39.0 2.0 39.0 14-15 30.349098652067703 36.5 27.0 40.0 2.0 41.0 16-17 30.756917604548363 37.0 28.0 40.0 2.0 41.0 18-19 30.555161802768566 37.0 27.0 40.0 2.0 41.0 20-21 30.4197998458675 37.0 27.0 40.0 2.0 41.0 22-23 30.50153350927703 37.0 27.0 40.0 2.0 41.0 24-25 30.572521520386204 37.0 27.0 40.0 2.0 41.0 26-27 30.34904503286221 37.0 27.0 40.0 2.0 41.0 28-29 30.23599793520619 37.0 27.0 40.0 2.0 41.0 30-31 30.111273301634387 37.0 27.0 40.0 2.0 41.0 32-33 30.003886210957948 36.5 27.0 40.0 2.0 41.0 34-35 30.061595562147385 37.0 27.0 40.0 2.0 41.0 36-37 30.033051968824523 37.0 27.0 40.0 2.0 41.0 38-39 29.745207170069214 36.0 26.0 40.0 2.0 41.0 40-41 29.611832940731695 36.0 25.5 40.0 2.0 41.0 42-43 29.137059050194846 35.5 24.5 40.0 2.0 41.0 44-45 29.04602254551271 35.0 24.0 40.0 2.0 41.0 46-47 29.0287324419822 35.0 24.5 40.0 2.0 41.0 48-49 28.970713735241088 35.0 24.0 40.0 2.0 41.0 50-51 28.76053971819927 35.0 24.0 40.0 2.0 41.0 52-53 28.661283661955448 35.0 23.5 40.0 2.0 41.0 54-55 28.492801030942825 35.0 23.5 39.5 2.0 41.0 56-57 28.277321438957713 35.0 23.0 39.0 2.0 41.0 58-59 28.06693094573373 34.5 22.5 39.0 2.0 41.0 60-61 27.782950910254172 34.0 22.0 39.0 2.0 40.0 62-63 27.597619489036234 34.0 21.5 39.0 2.0 40.0 64-65 27.383369685337055 34.0 21.5 38.0 2.0 40.0 66-67 27.136292022916304 34.0 20.0 38.0 2.0 40.0 68-69 26.895658116675392 33.5 20.0 37.5 2.0 40.0 70-71 26.49098961059734 33.0 18.0 37.0 2.0 40.0 72-73 26.25674911301111 33.0 18.0 36.5 2.0 39.0 74-75 26.21564226720177 33.0 18.0 36.0 2.0 39.0 76-77 25.958994394521028 33.0 18.0 36.0 2.0 39.0 78-79 25.67449052666783 33.0 16.5 35.5 2.0 37.5 80-81 25.288165968708192 32.0 12.5 35.0 2.0 37.0 82-83 25.380168891409298 32.5 15.0 35.0 2.0 37.0 84-85 25.217138152154945 33.0 12.0 35.0 2.0 36.0 86-87 25.07829894433781 33.0 11.0 35.0 2.0 36.0 88-89 24.91328156808003 32.5 8.5 35.0 2.0 36.0 90-91 24.780777969231664 32.0 7.0 35.0 2.0 35.0 92-93 24.65975360611877 32.0 7.0 35.0 2.0 35.0 94-95 24.55583358779736 32.0 4.5 35.0 2.0 35.0 96-97 24.442509124352934 32.0 2.0 35.0 2.0 35.0 98-99 24.35682345285872 32.0 2.0 35.0 2.0 35.0 100-101 24.258222277961963 32.0 2.0 35.0 2.0 35.0 102-103 23.203863127435586 30.0 2.0 34.0 2.0 35.0 104-105 23.996891721921713 32.0 2.0 34.5 2.0 35.0 106-107 24.15205625109056 32.0 2.0 35.0 2.0 35.0 108-109 24.10661951811784 32.0 2.0 35.0 2.0 35.0 110-111 24.03938030739254 32.0 2.0 35.0 2.0 35.0 112-113 23.953023577909615 32.0 2.0 35.0 2.0 35.0 114-115 23.855411177514107 32.0 2.0 35.0 2.0 35.0 116-117 23.7488398258012 32.0 2.0 35.0 2.0 35.0 118-119 23.622648025359158 32.0 2.0 35.0 2.0 35.0 120-121 23.441035777642064 32.0 2.0 35.0 2.0 35.0 122-123 23.235185467922992 32.0 2.0 35.0 2.0 35.0 124-125 22.338386625370788 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7816935848974431 1101 2 2.1269593451372018 1101 3 1.7053998375306136 1101 4 1.7246556313938584 1101 5 1.6226282412657298 1101 6 1.507341786483945 1101 7 1.6757896776217898 1101 8 1.753120840270423 1101 9 2.2327182326317647 1101 10-11 2.2599309984809786 1101 12-13 2.3068209072560713 1101 14-15 2.7338427251769595 1101 16-17 2.256508358861552 1101 18-19 2.3159076449099842 1101 20-21 2.584366895826321 1101 22-23 2.368076997947952 1101 24-25 2.328553606167816 1101 26-27 2.596753649155211 1101 28-29 2.524796215847722 1101 30-31 2.6805961300108905 1101 32-33 2.563683520881817 1101 34-35 2.4867169148223134 1101 36-37 2.303196378122564 1101 38-39 2.2929021555013733 1101 40-41 2.463014870816238 1101 42-43 2.4700857530230493 1101 44-45 2.3486342471015575 1101 46-47 2.1861514319617825 1101 48-49 2.1599138341610065 1101 50-51 2.185357858130697 1101 52-53 2.010730523658367 1101 54-55 1.9991876797661767 1101 56-57 1.9261577560332164 1101 58-59 1.8982504080260014 1101 60-61 1.9870618496351788 1101 62-63 1.9643783898540974 1101 64-65 1.9747020168579361 1101 66-67 1.799127458226021 1101 68-69 1.6809652057098745 1101 70-71 1.6805194468471427 1101 72-73 1.4816235134839175 1101 74-75 1.559677151398386 1101 76-77 1.6084353716398994 1101 78-79 1.536288099598682 1101 80-81 1.70258864994857 1101 82-83 1.7031387344493396 1101 84-85 1.7233774033659657 1101 86-87 1.720618894099708 1101 88-89 1.6345536425010145 1101 90-91 1.7280763928620893 1101 92-93 1.7263222576225736 1101 94-95 1.6166047379536366 1101 96-97 1.584103256682912 1101 98-99 1.6913465479640486 1101 100-101 1.5801208618475897 1101 102-103 1.4779146726910355 1101 104-105 1.6820904416391933 1101 106-107 1.6611582713527504 1101 108-109 1.6215117157855978 1101 110-111 1.6494629945378456 1101 112-113 1.6316742340243309 1101 114-115 1.7043107569168008 1101 116-117 1.598254856224326 1101 118-119 1.545560202092254 1101 120-121 1.575750199039156 1101 122-123 1.555432705881369 1101 124-125 1.427810632546926 1102 1 1.3096454630367873 1102 2 1.8339158591527607 1102 3 1.4678784109050689 1102 4 1.2575485913529612 1102 5 1.3001530711412173 1102 6 1.2379497188845576 1102 7 1.25044210377553 1102 8 1.1434079275430982 1102 9 1.803582848614397 1102 10-11 1.7605250186950165 1102 12-13 2.0475718275769026 1102 14-15 2.2423674747628617 1102 16-17 2.066757365263342 1102 18-19 1.7846939001277846 1102 20-21 2.1958956153640443 1102 22-23 2.2162938621032353 1102 24-25 1.9333645592566029 1102 26-27 2.216908824263051 1102 28-29 1.7397129980889332 1102 30-31 1.7789319968877209 1102 32-33 2.074503652694645 1102 34-35 2.0437293861205426 1102 36-37 1.9337559789980077 1102 38-39 2.2208218301544207 1102 40-41 2.162937193272537 1102 42-43 2.4444662756346176 1102 44-45 1.9255630933495702 1102 46-47 1.8057745278744122 1102 48-49 1.8123587162960106 1102 50-51 1.8896332025549007 1102 52-53 1.7828179313180428 1102 54-55 1.6581273114648774 1102 56-57 1.732558647422909 1102 58-59 1.7696195686679275 1102 60-61 1.728470630323887 1102 62-63 1.516279879792716 1102 64-65 1.2678940152002518 1102 66-67 1.5168267069983337 1102 68-69 1.4430764571556978 1102 70-71 1.6982904128009686 1102 72-73 1.2253466969671223 1102 74-75 1.3765750294743881 1102 76-77 1.1749677392698956 1102 78-79 1.352260485357526 1102 80-81 1.4819505335726184 1102 82-83 1.5036431038038707 1102 84-85 1.378921819926564 1102 86-87 1.389761117340516 1102 88-89 1.361817548756438 1102 90-91 1.478928432480501 1102 92-93 1.5267165647988428 1102 94-95 1.4089236893225738 1102 96-97 1.1601743951249475 1102 98-99 1.3309208752582897 1102 100-101 1.406466118515393 1102 102-103 1.2485689346363316 1102 104-105 1.3638503425896609 1102 106-107 1.2339468864064997 1102 108-109 1.384701646700325 1102 110-111 1.3329210727978662 1102 112-113 1.2167120069431832 1102 114-115 1.3664356470737538 1102 116-117 1.3444137241855927 1102 118-119 1.4025687173461634 1102 120-121 1.3418364569332724 1102 122-123 1.262525344051035 1102 124-125 1.1917767253320015 1103 1 1.3121213101863702 1103 2 1.6399108319982503 1103 3 1.2497978154041185 1103 4 1.070692537581099 1103 5 1.221666224666894 1103 6 1.2031113148092345 1103 7 1.2069109655663937 1103 8 1.402382138839915 1103 9 1.8601610219248705 1103 10-11 2.011523485395262 1103 12-13 1.867200503397136 1103 14-15 2.176607721501288 1103 16-17 1.732550147059328 1103 18-19 1.746858685480511 1103 20-21 2.021223300151931 1103 22-23 1.8389056257858343 1103 24-25 1.8116229975476088 1103 26-27 1.9984294474092366 1103 28-29 1.9244251943748054 1103 30-31 2.010247660934631 1103 32-33 2.0858419019119765 1103 34-35 1.921038694819213 1103 36-37 1.7941207092248206 1103 38-39 1.789047292803673 1103 40-41 2.0551369935733206 1103 42-43 2.2398254433155493 1103 44-45 2.0447583725023932 1103 46-47 1.8370326858342594 1103 48-49 1.5948809797026868 1103 50-51 1.6579653614750463 1103 52-53 1.5090508741663342 1103 54-55 1.5117345034576353 1103 56-57 1.502105868530144 1103 58-59 1.5685861340353355 1103 60-61 1.6501223038773745 1103 62-63 1.475577676695437 1103 64-65 1.404175654567446 1103 66-67 1.381867002398021 1103 68-69 1.4331387764758077 1103 70-71 1.4737612604177706 1103 72-73 1.3487889343506012 1103 74-75 1.422080063387405 1103 76-77 1.4637210825274636 1103 78-79 1.4063396268977293 1103 80-81 1.5632649046875748 1103 82-83 1.5102952974686232 1103 84-85 1.5597376776194203 1103 86-87 1.4628206468660885 1103 88-89 1.5099214640482401 1103 90-91 1.4330447164295137 1103 92-93 1.3492396157186128 1103 94-95 1.4302804274668297 1103 96-97 1.4018036598169132 1103 98-99 1.3792027450024484 1103 100-101 1.317374849861178 1103 102-103 1.2247278865151685 1103 104-105 1.2983547294490059 1103 106-107 1.3422886776696856 1103 108-109 1.5019249989320151 1103 110-111 1.479778807381269 1103 112-113 1.5167727747449433 1103 114-115 1.5612372084653927 1103 116-117 1.5217992864846366 1103 118-119 1.6030453096947106 1103 120-121 1.5837599811836824 1103 122-123 1.4830124973028482 1103 124-125 1.310553065147431 1104 1 1.0570408162289162 1104 2 1.492071967257452 1104 3 0.963704889129378 1104 4 1.1463106554462499 1104 5 0.7122421866452164 1104 6 0.7967611185500658 1104 7 0.7105561376708067 1104 8 0.9704891245012597 1104 9 1.2742202371965945 1104 10-11 1.3976487360376062 1104 12-13 1.4341008364066532 1104 14-15 1.3882854418452517 1104 16-17 1.2648589235820111 1104 18-19 1.2501320416921864 1104 20-21 1.5099121986734083 1104 22-23 1.3577926224237586 1104 24-25 1.4460869698697252 1104 26-27 1.5767254906439376 1104 28-29 1.5438507115504123 1104 30-31 1.7522131138384296 1104 32-33 1.6508856683061737 1104 34-35 1.4696403518142667 1104 36-37 1.3575360642131962 1104 38-39 1.554973268859655 1104 40-41 1.5769497166956938 1104 42-43 1.7863528582056603 1104 44-45 1.5849258538459239 1104 46-47 1.3775372886300339 1104 48-49 1.221735161967576 1104 50-51 1.2764969899549818 1104 52-53 1.166870144400903 1104 54-55 1.2625530352574437 1104 56-57 1.2267359515512268 1104 58-59 1.1841016305998693 1104 60-61 1.1983097121084363 1104 62-63 0.9960384302872392 1104 64-65 1.0315069639156391 1104 66-67 0.8956991475538523 1104 68-69 0.8276685054708182 1104 70-71 0.8804717077561826 1104 72-73 0.9025705998093905 1104 74-75 0.8200652729903695 1104 76-77 0.761972397061264 1104 78-79 0.7692490998220727 1104 80-81 0.8489970087811791 1104 82-83 0.935999566055564 1104 84-85 0.9755483196464674 1104 86-87 0.9160991429948702 1104 88-89 0.8275692266708745 1104 90-91 0.8986176372902115 1104 92-93 0.9095282734635255 1104 94-95 0.8737240634213457 1104 96-97 0.8465341025750845 1104 98-99 0.8328537994564442 1104 100-101 0.9243499172654772 1104 102-103 0.8199288886847853 1104 104-105 0.8958443082670655 1104 106-107 0.9613673932158235 1104 108-109 0.9003225155525065 1104 110-111 0.8288900300476207 1104 112-113 0.8682709178071164 1104 114-115 0.8698882886189736 1104 116-117 0.8697591106080935 1104 118-119 0.8828088563745808 1104 120-121 0.8689586475465205 1104 122-123 0.9101335554861407 1104 124-125 0.7415288041675687 1105 1 1.1392570291090074 1105 2 1.4820546249272901 1105 3 0.93293549247635 1105 4 1.1780600754264263 1105 5 0.835024098349443 1105 6 0.8662667922230227 1105 7 0.8557971559628363 1105 8 0.945309415650911 1105 9 1.361108394414103 1105 10-11 1.1652680824202868 1105 12-13 1.3577718394701606 1105 14-15 1.6308718778978495 1105 16-17 1.124566430619101 1105 18-19 1.2919775383274725 1105 20-21 1.1854422331508445 1105 22-23 1.362055507686339 1105 24-25 1.0872482079394672 1105 26-27 1.4281164459344637 1105 28-29 1.417755275327714 1105 30-31 1.401634071174815 1105 32-33 1.5386683642649288 1105 34-35 1.323247970376677 1105 36-37 0.9218135990021707 1105 38-39 1.255911016010046 1105 40-41 1.414228302380117 1105 42-43 1.641809554074225 1105 44-45 1.2917009762028258 1105 46-47 0.8238778525486303 1105 48-49 0.871399987924562 1105 50-51 1.0599217747230263 1105 52-53 0.8913363416829512 1105 54-55 0.9833136088364398 1105 56-57 1.0219738614660194 1105 58-59 1.0451817374965984 1105 60-61 0.9549880479658164 1105 62-63 0.8819400385509546 1105 64-65 0.6014011145449629 1105 66-67 0.4979276378220483 1105 68-69 0.4523506268655595 1105 70-71 0.8260974572274904 1105 72-73 0.6355546492572408 1105 74-75 0.7458667639893584 1105 76-77 0.7515461922112863 1105 78-79 0.6452292003672682 1105 80-81 0.8929295827719308 1105 82-83 0.8567720564845942 1105 84-85 0.7622774189263488 1105 86-87 0.8600639463929589 1105 88-89 0.8640754185190183 1105 90-91 0.7781584162512303 1105 92-93 0.8277937888569014 1105 94-95 0.6808144937246503 1105 96-97 0.7079519190745742 1105 98-99 0.8201502239128509 1105 100-101 0.8457086751590559 1105 102-103 0.6681045127287213 1105 104-105 0.8156034452535046 1105 106-107 0.8547649079404636 1105 108-109 0.8229629806488568 1105 110-111 0.8618926567527403 1105 112-113 0.8917026347899011 1105 114-115 0.9316143427000192 1105 116-117 0.9106364895345536 1105 118-119 0.9242169900122263 1105 120-121 0.8207399667216038 1105 122-123 0.8210218836503564 1105 124-125 0.8187696479187387 1106 1 0.7563123310356517 1106 2 -1.3342330190397114 1106 3 0.24780632623403775 1106 4 0.7144231180287051 1106 5 0.8405803058124803 1106 6 0.8474053437190392 1106 7 1.0323899811920967 1106 8 0.9996172448875598 1106 9 1.486240073575253 1106 10-11 1.6938590102297724 1106 12-13 1.7227038356797486 1106 14-15 1.8325669911009648 1106 16-17 1.4359502573849028 1106 18-19 1.4629614346136677 1106 20-21 1.7355447978778784 1106 22-23 1.3608730797417934 1106 24-25 1.4804641850110158 1106 26-27 1.5123239964253123 1106 28-29 1.6437763561706404 1106 30-31 1.791472331083689 1106 32-33 1.7940988704354162 1106 34-35 1.6538256538861766 1106 36-37 1.3241348939413555 1106 38-39 1.7407790102801464 1106 40-41 1.6782980463945023 1106 42-43 1.8838884076961513 1106 44-45 1.7120504988589502 1106 46-47 1.5273856713793599 1106 48-49 1.444737725323435 1106 50-51 1.4738812325967636 1106 52-53 1.3498026641424659 1106 54-55 1.2682732637584415 1106 56-57 1.274469593932043 1106 58-59 1.330400820845803 1106 60-61 1.4542119490343062 1106 62-63 1.3550422036463985 1106 64-65 1.3456448422109801 1106 66-67 1.3203376985749067 1106 68-69 1.1829032088619904 1106 70-71 1.2549223244966683 1106 72-73 1.0670205167345372 1106 74-75 1.044925055957318 1106 76-77 0.926636773525793 1106 78-79 0.9519778265645016 1106 80-81 1.0735195541224414 1106 82-83 0.9942488368931279 1106 84-85 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-3.1135938562965926 2216 98-99 -3.0940831946916063 2216 100-101 -3.149852709072828 2216 102-103 -2.8794469878267677 2216 104-105 -2.991149597256989 2216 106-107 -3.149384832172082 2216 108-109 -3.097444167829753 2216 110-111 -2.9484827821870034 2216 112-113 -3.322955702125533 2216 114-115 -3.239729539905891 2216 116-117 -3.033000280844764 2216 118-119 -3.1748063095948567 2216 120-121 -3.3786273855294624 2216 122-123 -3.2147973558769323 2216 124-125 -3.126916605735129 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 322393.0 3 37930.0 4 21336.0 5 17398.0 6 17078.0 7 19050.0 8 20878.0 9 21846.0 10 22814.0 11 23562.0 12 23973.0 13 23913.0 14 23216.0 15 20049.0 16 17412.0 17 15479.0 18 13983.0 19 13159.0 20 12893.0 21 14102.0 22 17640.0 23 24400.0 24 34550.0 25 43869.0 26 49762.0 27 55791.0 28 64000.0 29 71718.0 30 83390.0 31 95143.0 32 114084.0 33 141045.0 34 180992.0 35 242371.0 36 343954.0 37 385088.0 38 100473.0 39 146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.90304667370367 18.841837110078146 13.9240192811036 23.331096935114584 2 13.627991425663764 19.90250208692465 42.57089440616746 23.89861208124413 3 19.354762273642766 25.61138097277789 27.914041211370037 27.119815542209302 4 12.269087115679786 16.82780435157406 37.5634086661882 33.33969986655795 5 13.512065926709695 39.013600555952166 33.66128482995842 13.813048687379725 6 32.04420090778569 37.31178420071106 17.244867885086677 13.399147006416579 7 28.452715956964337 33.09429487844993 20.328905548482513 18.12408361610322 8 25.375701195993976 37.39850367816042 19.334762494782687 17.89103263106292 9 25.58359583536834 17.81856165274122 19.823192177712514 36.77465033417793 10-11 24.36289583997647 28.172325527556097 27.237445016664264 20.22733361580317 12-13 25.10855901818556 25.00240756804679 27.68863454937517 22.20039886439248 14-15 21.984246635203636 26.555034813261084 25.377010138087748 26.083708413447532 16-17 22.137912178726857 29.88860516541758 25.75598713560404 22.21749552025152 18-19 22.166236302982345 28.597639511938084 27.664998741864448 21.571125443215116 20-21 22.858822015060767 27.681846807374868 27.78500474005785 21.67432643750652 22-23 22.555264676060276 27.46005020220738 27.46789884969057 22.51678627204177 24-25 22.44687780013995 27.452346750576712 27.576511135555133 22.524264313728203 26-27 22.480673678564937 27.57254042796457 27.567785589962448 22.37900030350805 28-29 22.275881077719514 27.702873438083703 27.695791610018112 22.32545387417867 30-31 22.41663601206332 27.48604889435019 27.697460997970698 22.399854095615787 32-33 22.295372110300224 27.44255196725371 27.64439394601524 22.617681976430827 34-35 22.647199617041647 27.389949527399544 27.543333084580286 22.41951777097853 36-37 22.58158048875669 27.3984887574167 27.40549637405893 22.61443437976768 38-39 22.373648150046403 27.516481467691335 27.672078830656506 22.43779155160576 40-41 22.637820942402275 27.367369058996292 27.6211510221207 22.373658976480733 42-43 22.19763330452275 27.22149907235757 27.919440436900363 22.66142718621932 44-45 22.582630068047596 27.428138051110608 27.454061638061216 22.53517024278058 46-47 22.587310743371113 27.70368107886039 27.00971659491605 22.699291582852442 48-49 22.59512146463456 27.484159695245808 27.1517631252595 22.768955714860134 50-51 22.52847471634504 27.602171305204177 27.18027039375841 22.68908358469237 52-53 22.76499056776742 27.642968739900702 26.90026121849406 22.691779473837823 54-55 22.783000465499768 27.502370735520582 26.986461548208172 22.728167250771477 56-57 22.545868419749564 27.751304180659968 27.040394647543536 22.66243275204693 58-59 22.797723298047355 27.459636916525966 26.937181393769226 22.805458391657456 60-61 22.75579265662207 27.455865430016495 27.15942033206365 22.628921581297785 62-63 22.657836164060534 27.551663190633036 27.08790891778718 22.70259172751925 64-65 22.66925176619955 27.424095005865794 27.029596150087194 22.87705707784746 66-67 22.548972685938796 27.67967418571451 26.85876922488993 22.912583903456774 68-69 22.600539359425607 27.459250956817943 27.04254766619406 22.897662017562393 70-71 22.58174880309459 27.390862681810606 27.147714542155022 22.87967397293978 72-73 22.807682440681422 27.424078949523036 26.765802367830688 23.002436241964862 74-75 22.540810311199092 27.63240153125091 26.90613082588571 22.92065733166429 76-77 22.772996519896175 27.393620070497445 26.984444447275322 22.848938962331054 78-79 22.710673109297225 27.453977960379643 26.898401172049173 22.936947758273956 80-81 22.500283940748115 27.52883818726402 27.15119699226662 22.819680879721243 82-83 22.681347411967128 27.603208518967698 26.965785760062932 22.749658309002243 84-85 22.69262114132569 27.360011414640077 27.027199675032186 22.920167769002045 86-87 22.570927918150417 27.257283930480863 27.25549996959985 22.916288181768866 88-89 22.63075482510079 27.37337728294419 27.160182351566302 22.835685540388717 90-91 22.60254263247587 27.57090305879244 27.08577566962559 22.7407786391061 92-93 22.645160949789314 27.489061704563095 27.092245335969796 22.77353200967779 94-95 22.557630817605027 27.40304689474079 27.09555024623762 22.943772041416562 96-97 22.593831445060864 27.47688548444464 27.053239199422467 22.876043871072024 98-99 22.466290498725883 27.38596650891882 26.988369129959956 23.15937386239534 100-101 22.358217147116555 27.449864951379947 27.18062302527107 23.011294876232423 102-103 22.386933717383037 27.273318103148362 27.215244603215087 23.12450357625351 104-105 22.30011171756983 27.235575212639173 27.34501803149602 23.119295038294975 106-107 22.28601368490362 27.334282834687397 27.29763009266556 23.082073387743424 108-109 22.303038730647327 27.378389072698823 27.023706081429115 23.294866115224742 110-111 22.158242486111337 27.46410238213517 27.245304786288393 23.1323503454651 112-113 22.32893190027025 27.411034144205992 27.25091807461154 23.00911588091222 114-115 22.169949410179644 27.433947124949853 27.17150958895885 23.22459387591166 116-117 21.991794185403958 27.3821253420075 27.344660770955002 23.28141970163354 118-119 22.07053709935313 27.41635219393509 27.209503331685696 23.303607375026083 120-121 22.107092799145747 27.485926913635044 27.25211676136648 23.154863525852733 122-123 22.120864744083132 27.436591681177937 27.227829605894033 23.2147139688449 124-125 22.12365662320129 27.33064104535253 27.170632166078807 23.375070165367376 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3248.0 1 2283.5 2 1051.5 3 684.5 4 565.0 5 551.0 6 537.5 7 626.5 8 799.5 9 863.0 10 875.5 11 921.0 12 951.5 13 1037.5 14 1194.5 15 1262.0 16 1372.0 17 1559.5 18 1765.0 19 2022.0 20 2450.5 21 3138.0 22 4026.0 23 5101.5 24 6522.5 25 8853.0 26 12029.5 27 16021.5 28 20366.5 29 25194.5 30 31205.5 31 37043.5 32 42066.5 33 47522.5 34 53707.5 35 60288.0 36 67714.5 37 74630.0 38 79730.5 39 83867.5 40 88065.0 41 92748.5 42 96794.5 43 101294.0 44 105639.5 45 107209.0 46 106675.5 47 106880.5 48 107737.5 49 106377.5 50 103580.5 51 100006.0 52 96231.5 53 92531.5 54 89151.5 55 83387.5 56 75119.0 57 68066.0 58 61802.5 59 54500.0 60 45874.5 61 37063.0 62 29082.0 63 22425.5 64 17016.0 65 13008.0 66 9708.0 67 7241.0 68 5676.5 69 4281.5 70 3066.0 71 2242.0 72 1654.5 73 1269.5 74 967.0 75 708.5 76 516.0 77 340.0 78 242.5 79 174.5 80 121.5 81 89.0 82 60.5 83 46.5 84 43.5 85 34.0 86 21.5 87 15.5 88 15.0 89 13.0 90 10.5 91 9.0 92 6.5 93 5.0 94 2.5 95 1.0 96 0.5 97 0.5 98 1.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1960827080788693 2 0.23305269586459607 3 0.2800921886814401 4 0.26780521142325364 5 0.2980137265166056 6 0.21662159018205085 7 0.20775170127377418 8 0.1895393474088292 9 0.18855784330832315 10-11 0.13392078171348804 12-13 0.15749505612749373 14-15 0.17448961786773687 16-17 0.14809806316524166 18-19 0.17278107369278195 20-21 0.16741915314372127 22-23 0.1884487872971558 24-25 0.17897909032745885 26-27 0.22927208747746175 28-29 0.16045774443087302 30-31 0.1408821904263363 32-33 0.1788518583144303 34-35 0.13977345431280172 36-37 0.14064590240214042 38-39 0.17047272145640666 40-41 0.18961205141627407 42-43 0.25797199441633223 44-45 0.15800398417960798 46-47 0.1447355028209155 48-49 0.1457897109288664 50-51 0.15067905542953527 52-53 0.17127246553830047 54-55 0.15884008026522423 56-57 0.15733147211074275 58-59 0.13306650962601058 60-61 0.14529895887861338 62-63 0.13579290990519396 64-65 0.1307581573896353 66-67 0.12005249229337521 68-69 0.12219726051299948 70-71 0.12279706857441983 72-73 0.10160385040423427 74-75 0.10178561042284649 76-77 0.12376039667306461 78-79 0.12923137323329262 80-81 0.13915547024952016 82-83 0.14106395044494852 84-85 0.12835892514395394 86-87 0.1520604315709882 88-89 0.14897051125458033 90-91 0.13691982202058978 92-93 0.13688347001686732 94-95 0.15798580817774677 96-97 0.14588059093817252 98-99 0.1410457744430873 100-101 0.1218155644739138 102-103 0.1585129122317222 104-105 0.1209249403827139 106-107 0.12625050892805212 108-109 0.13737422206712033 110-111 0.11396353166986564 112-113 0.10445748269644624 114-115 0.1016947304135404 116-117 0.10725658698307451 118-119 0.0907527772930844 120-121 0.08297344849648113 122-123 0.10960129122317222 124-125 0.10569345082300936 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2750880.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.38705357100284 #Duplication Level Percentage of deduplicated Percentage of total 1 82.1687515695908 53.727725607432575 2 10.127828229325942 13.244576939777005 3 3.300638692708361 6.474571170559392 4 1.4626403700460044 3.825509769252381 5 0.8111379200420648 2.6518959315631156 6 0.47133837593399863 1.8491656582359506 7 0.317481831118221 1.4531441049403035 8 0.22716355479731576 1.188284442152922 9 0.17295077690927335 1.0177867543421897 >10 0.8690595698379241 10.048275892593594 >50 0.05221020154915578 2.3093142945249503 >100 0.018282433714386603 1.8516670109623217 >500 3.4431628433427764E-4 0.1597731279447602 >1k 1.721581421622899E-4 0.198309295718537 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.6352003722445184E-5 0.0 0.0 0.0 0.0 3 3.6352003722445184E-5 0.0 0.0 0.0 0.0 4 3.6352003722445184E-5 0.0 0.0 0.0 0.0 5 7.270400744489037E-5 7.270400744489037E-5 0.0 0.0 0.0 6 7.270400744489037E-5 7.270400744489037E-5 0.0 0.0 0.0 7 1.0905601116733554E-4 7.270400744489037E-5 0.0 0.0 0.0 8 1.0905601116733554E-4 7.270400744489037E-5 0.0 0.0 0.0 9 1.0905601116733554E-4 7.270400744489037E-5 0.0 0.0 0.0 10-11 1.0905601116733554E-4 7.270400744489037E-5 0.0 0.0 0.0 12-13 1.0905601116733554E-4 7.270400744489037E-5 0.0 1.8176001861222592E-5 0.0 14-15 1.0905601116733554E-4 7.270400744489037E-5 0.0 3.6352003722445184E-5 0.0 16-17 1.817600186122259E-4 7.270400744489037E-5 0.0 3.6352003722445184E-5 0.0 18-19 2.5446402605711624E-4 7.270400744489037E-5 0.0 3.6352003722445184E-5 0.0 20-21 2.9081602977956147E-4 7.270400744489037E-5 0.0 9.088000930611295E-5 0.0 22-23 2.9081602977956147E-4 7.270400744489037E-5 0.0 1.0905601116733554E-4 0.0 24-25 3.635200372244518E-4 7.270400744489037E-5 0.0 1.817600186122259E-4 0.0 26-27 3.635200372244518E-4 7.270400744489037E-5 0.0 2.7264002791833886E-4 0.0 28-29 3.816960390856744E-4 7.270400744489037E-5 0.0 6.725120688652359E-4 0.0 30-31 4.7257604839178737E-4 7.270400744489037E-5 0.0 0.0016540161693712559 0.0 32-33 5.271040539754552E-4 7.270400744489037E-5 0.0 0.00407142441691386 0.0 34-35 6.906880707264584E-4 7.270400744489037E-5 0.0 0.00701593671843192 0.0 36-37 7.270400744489036E-4 7.270400744489037E-5 0.0 0.012268801256325249 0.0 38-39 7.633920781713488E-4 7.270400744489037E-5 0.0 0.02219289827255278 0.0 40-41 7.633920781713488E-4 7.270400744489037E-5 0.0 0.03444352352701681 0.0 42-43 7.997440818937941E-4 7.270400744489037E-5 0.0 0.04553088466236259 0.0 44-45 9.088000930611295E-4 7.270400744489037E-5 0.0 0.05967181411039376 0.0 46-47 9.996801023672425E-4 7.270400744489037E-5 0.0 0.07655731983946955 0.0 48-49 0.0010905601116733554 7.270400744489037E-5 0.0 0.09627828185889606 0.0 50-51 0.0010905601116733554 7.270400744489037E-5 0.0 0.11961626824870586 0.0 52-53 0.0010905601116733554 7.270400744489037E-5 0.0 0.14126388646542198 1.8176001861222592E-5 54-55 0.0010905601116733554 7.270400744489037E-5 0.0 0.1659105449892398 3.6352003722445184E-5 56-57 0.0010905601116733554 7.270400744489037E-5 0.0 0.19491944395975108 3.6352003722445184E-5 58-59 0.0010905601116733554 7.270400744489037E-5 0.0 0.22827240737509452 3.6352003722445184E-5 60-61 0.0010905601116733554 7.270400744489037E-5 0.0 0.2660603152445763 3.6352003722445184E-5 62-63 0.0010905601116733554 7.270400744489037E-5 0.0 0.30586575932065374 7.270400744489037E-5 64-65 0.0010905601116733554 7.270400744489037E-5 0.0 0.34756150759029836 7.270400744489037E-5 66-67 0.0011269121153958006 7.270400744489037E-5 0.0 0.39670941662304426 7.270400744489037E-5 68-69 0.0011269121153958006 7.270400744489037E-5 0.0 0.45550878264409933 7.270400744489037E-5 70-71 0.0011814401209794684 7.270400744489037E-5 0.0 0.5198336532309661 7.270400744489037E-5 72-73 0.0012177921247019134 7.270400744489037E-5 0.0 0.587521084162159 7.270400744489037E-5 74-75 0.001235968126563136 7.270400744489037E-5 0.0 0.6577349793520619 1.0905601116733554E-4 76-77 0.001235968126563136 7.270400744489037E-5 0.0 0.7396178677368697 1.0905601116733554E-4 78-79 0.0012541441284243588 7.270400744489037E-5 0.0 0.8334969173500844 1.0905601116733554E-4 80-81 0.0013632001395916943 7.270400744489037E-5 0.0 0.9421712324783342 1.0905601116733554E-4 82-83 0.001417728145175362 7.270400744489037E-5 0.0 1.059588204501832 1.0905601116733554E-4 84-85 0.0015086081544814752 7.270400744489037E-5 0.0 1.1773505205606933 1.0905601116733554E-4 86-87 0.0015449601582039202 7.270400744489037E-5 0.0 1.3026195253882396 1.0905601116733554E-4 88-89 0.0015631361600651427 7.270400744489037E-5 0.0 1.4463371721049265 1.0905601116733554E-4 90-91 0.0016358401675100331 7.270400744489037E-5 0.0 1.6139562612691212 1.0905601116733554E-4 92-93 0.0016358401675100331 7.270400744489037E-5 0.0 1.7992060722387018 1.0905601116733554E-4 94-95 0.0016358401675100331 7.270400744489037E-5 0.0 1.9837106671319722 1.0905601116733554E-4 96-97 0.0016358401675100331 7.270400744489037E-5 0.0 2.174304222648752 1.0905601116733554E-4 98-99 0.001690368173093701 7.270400744489037E-5 0.0 2.385182196242657 1.0905601116733554E-4 100-101 0.001781248182399814 7.270400744489037E-5 0.0 2.633102861629733 1.0905601116733554E-4 102-103 0.0018539521898447043 7.270400744489037E-5 0.0 2.905343017507125 1.0905601116733554E-4 104-105 0.002217472227069156 7.270400744489037E-5 0.0 3.1809275577269815 1.0905601116733554E-4 106-107 0.0022901762345140463 7.270400744489037E-5 0.0 3.4572936660268714 1.0905601116733554E-4 108-109 0.0022901762345140463 7.270400744489037E-5 0.0 3.7504907520502533 1.0905601116733554E-4 110-111 0.0023265282382364917 7.270400744489037E-5 0.0 4.082929826092014 1.0905601116733554E-4 112-113 0.0023265282382364917 7.270400744489037E-5 0.0 4.4538111440702615 1.0905601116733554E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5545 0.0 46.519905 1 ATCAACG 6425 0.0 39.590538 3 TCAACGC 6405 0.0 38.9701 4 CAACGCA 6485 0.0 38.49076 5 TATCAAC 7010 0.0 36.626537 2 AACGCAG 6715 0.0 36.516083 6 GTACATG 9295 0.0 33.26369 1 GGTATCA 3430 0.0 32.478798 1 TACATGG 9670 0.0 31.418133 2 ACGCAGA 7805 0.0 30.498646 7 ACATGGG 10210 0.0 28.939604 3 CGCAGAG 7815 0.0 27.943138 8 CGCATAG 280 9.458745E-11 25.501242 8 CATGGGG 5440 0.0 24.091309 4 GCAGAGT 9145 0.0 21.927256 9 GTGTAGC 1755 0.0 20.36698 1 GTACCTG 2670 0.0 19.634682 1 TAGCCCT 2090 0.0 19.38203 4 TACCTGG 2520 0.0 19.1479 2 AGTACAT 7210 0.0 18.755438 2 >>END_MODULE