FastQCFastQC Report
Thu 26 May 2016
SRR1364613_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364613_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2750880
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55760.20269877275635434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46790.170091025417321No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27830.10116762635956494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52150.049.477671
GTATCAA80800.043.733981
ATCAACG98900.035.4335823
TATCAAC102250.034.535572
TCAACGC101350.034.4644
CAACGCA101150.034.333045
AACGCAG102850.034.047086
GTACATG97650.031.1834561
TACATGG98500.030.1675152
ACATGGG101550.028.298493
ACGCAGA123850.027.7932077
CGCAGAG124100.027.6412438
GAGTACT77700.024.2952512-13
GCAGAGT139900.023.9661089
CATGGGG56650.023.4237424
AGGACCG9550.023.0785185
GTACTTT84600.022.13774714-15
GGACCGA7850.021.2419916
GTGGTAT31150.021.2344261
AGTACTT85500.020.6510312-13
Produced by FastQC (version 0.11.5)