##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364613_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2750880 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.75927412318967 31.0 30.0 33.0 26.0 34.0 2 29.789123480486243 31.0 30.0 34.0 26.0 34.0 3 29.831347786890014 31.0 30.0 34.0 26.0 34.0 4 33.55477156400861 35.0 33.0 37.0 28.0 37.0 5 33.039422657476884 35.0 33.0 37.0 26.0 37.0 6 33.0782011574478 35.0 33.0 37.0 26.0 37.0 7 32.86422235793637 35.0 33.0 37.0 26.0 37.0 8 32.806042430058746 35.0 33.0 37.0 25.0 37.0 9 34.115847292502764 38.0 34.0 39.0 25.0 39.0 10-11 33.92617853196068 38.0 33.0 39.0 25.0 39.0 12-13 33.816523439772 37.5 33.0 39.0 24.0 39.0 14-15 34.83798039172919 39.0 34.0 41.0 24.5 41.0 16-17 34.7529208834991 39.0 33.5 41.0 24.0 41.0 18-19 34.77412573431047 39.0 33.5 41.0 24.0 41.0 20-21 34.78266173006456 39.0 34.0 41.0 24.0 41.0 22-23 34.78246379340429 39.0 34.0 41.0 24.0 41.0 24-25 34.78787787907869 39.0 34.0 41.0 24.0 41.0 26-27 34.702976683824815 39.0 34.0 41.0 23.5 41.0 28-29 34.47011047373931 39.0 33.0 41.0 22.0 41.0 30-31 34.42343668207992 38.5 33.0 41.0 22.5 41.0 32-33 34.27305789420113 38.0 33.0 41.0 21.5 41.0 34-35 34.17037856976677 38.0 33.0 41.0 21.0 41.0 36-37 34.03081668411563 38.0 33.0 41.0 19.0 41.0 38-39 33.910503547955564 38.0 33.0 40.5 18.0 41.0 40-41 33.747329581806554 38.0 33.0 40.0 16.0 41.0 42-43 33.597844871459316 38.0 33.0 40.0 15.5 41.0 44-45 33.42750774297679 38.0 32.5 40.0 14.5 41.0 46-47 33.22015282382365 38.0 32.0 40.0 10.5 41.0 48-49 33.00332039202001 38.0 32.0 40.0 8.5 41.0 50-51 32.81968642761589 38.0 32.0 40.0 7.5 41.0 52-53 32.6609072369569 38.0 31.0 40.0 6.5 41.0 54-55 32.454111775141044 38.0 31.0 40.0 2.0 41.0 56-57 32.241283516547426 37.0 31.0 40.0 2.0 41.0 58-59 32.057656277264 37.0 31.0 40.0 2.0 41.0 60-61 31.844456864712384 37.0 30.5 40.0 2.0 41.0 62-63 31.573709685627872 36.0 30.0 40.0 2.0 41.0 64-65 31.303234783051245 36.0 30.0 39.0 2.0 41.0 66-67 31.030380641830977 35.5 29.5 39.0 2.0 41.0 68-69 30.71821326266504 35.0 29.0 39.0 2.0 40.5 70-71 30.455878846041994 35.0 29.0 38.0 2.0 40.0 72-73 30.296714869423603 35.0 29.0 37.5 2.0 40.0 74-75 30.00001581312162 35.0 29.0 37.0 2.0 39.0 76-77 29.69998200575816 35.0 29.0 36.5 2.0 39.0 78-79 29.387946402605714 34.0 29.0 36.0 2.0 39.0 80-81 29.098308541266796 34.0 29.0 36.0 2.0 37.5 82-83 28.8330341563427 34.0 29.0 35.0 2.0 37.0 84-85 28.8089502268365 34.0 29.0 35.0 2.0 37.0 86-87 28.660127849997092 34.0 29.0 35.0 2.0 36.0 88-89 28.51897538242308 34.0 29.0 35.0 2.0 36.0 90-91 28.365823300180306 34.0 29.0 35.0 2.0 36.0 92-93 28.23062129209562 34.0 29.0 35.0 2.0 35.5 94-95 28.148520655208515 34.0 29.0 35.0 2.0 35.0 96-97 28.06979239370674 34.0 29.0 35.0 2.0 35.0 98-99 27.989134204327343 34.0 29.0 35.0 2.0 35.0 100-101 27.893954480020938 34.0 28.0 35.0 2.0 35.0 102-103 26.702690593555516 32.5 25.5 34.5 2.0 35.0 104-105 27.643570057581574 34.0 27.0 35.0 2.0 35.0 106-107 27.82114668760542 34.0 28.0 35.0 2.0 35.0 108-109 27.8366220991101 34.0 29.0 35.0 2.0 35.0 110-111 27.787164289245624 34.0 29.0 35.0 2.0 35.0 112-113 27.738640180596754 34.0 29.0 35.0 2.0 35.0 114-115 27.663760869249113 34.0 28.0 35.0 2.0 35.0 116-117 27.55019393793986 34.0 28.0 35.0 2.0 35.0 118-119 27.45032444163322 34.0 27.0 35.0 2.0 35.0 120-121 27.319218940848017 34.0 27.0 35.0 2.0 35.0 122-123 27.14268270517071 34.0 27.0 35.0 2.0 35.0 124-125 26.338821213575294 33.0 25.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7730467442517437 1101 2 0.8061945199485656 1101 3 0.669647979047479 1101 4 0.7629803681018288 1101 5 1.0729758082472713 1101 6 1.1492863259364299 1101 7 1.0441844263970097 1101 8 1.2439978466879396 1101 9 1.3531597542459082 1101 10-11 1.3290421789454854 1101 12-13 1.2729325407826337 1101 14-15 1.4686443153432265 1101 16-17 1.5357370976641747 1101 18-19 1.5586957219346331 1101 20-21 1.629422503631588 1101 22-23 1.5357194298745824 1101 24-25 1.3413183526519887 1101 26-27 1.4268660980743917 1101 28-29 1.540480803409018 1101 30-31 1.4750886639481138 1101 32-33 1.5635483099480325 1101 34-35 1.5313892532162825 1101 36-37 1.6499168498478767 1101 38-39 1.7476343738252282 1101 40-41 1.7709177144691282 1101 42-43 1.7676574280702937 1101 44-45 1.6984853444637622 1101 46-47 1.6488708419904512 1101 48-49 1.7410050629774645 1101 50-51 1.7333209802778597 1101 52-53 1.6765058749677806 1101 54-55 1.6230977938964273 1101 56-57 1.5934181979650788 1101 58-59 1.6740024318360938 1101 60-61 1.6215378509750629 1101 62-63 1.5719645361461758 1101 64-65 1.5574824356507158 1101 66-67 1.487292036367588 1101 68-69 1.4964241157427267 1101 70-71 1.5223208053651653 1101 72-73 1.570313814340576 1101 74-75 1.4737183723040168 1101 76-77 1.4681073589369333 1101 78-79 1.4280989715059142 1101 80-81 1.462485718415813 1101 82-83 1.4227475546216937 1101 84-85 1.506320176181724 1101 86-87 1.4881655177934832 1101 88-89 1.5458430927326923 1101 90-91 1.5750474727387136 1101 92-93 1.598398451490457 1101 94-95 1.4821635675696854 1101 96-97 1.4984547413637976 1101 98-99 1.5402448325678648 1101 100-101 1.5713840261651413 1101 102-103 1.584172427254746 1101 104-105 1.450074247745178 1101 106-107 1.5364862453742916 1101 108-109 1.4250082239777235 1101 110-111 1.4133767940088724 1101 112-113 1.4405905525225116 1101 114-115 1.3986353728550185 1101 116-117 1.4596546152538892 1101 118-119 1.512646253378179 1101 120-121 1.5092934652079997 1101 122-123 1.5745313095171838 1101 124-125 1.415477796510686 1102 1 0.6053586740802821 1102 2 -0.39309714359670167 1102 3 0.13747037942090046 1102 4 0.4897150000383661 1102 5 0.8904747771630426 1102 6 0.8621739846792877 1102 7 0.8518360588876277 1102 8 0.6961173222648895 1102 9 1.0031910110864501 1102 10-11 1.1615606177933984 1102 12-13 1.1959416366459408 1102 14-15 1.3738535199656141 1102 16-17 1.427282902404805 1102 18-19 1.4532202468966915 1102 20-21 1.3682872907116703 1102 22-23 1.4086076063508557 1102 24-25 1.3521008231566682 1102 26-27 1.3985960417770187 1102 28-29 1.4772065041518587 1102 30-31 1.341708117144485 1102 32-33 1.2462486917735092 1102 34-35 1.1459885818925102 1102 36-37 1.1827048022495958 1102 38-39 1.0146680079153256 1102 40-41 1.0955562038936364 1102 42-43 1.036362768449635 1102 44-45 0.9704323622199169 1102 46-47 0.9039092179577111 1102 48-49 1.0376168483431911 1102 50-51 0.9833068390263904 1102 52-53 1.035538722056323 1102 54-55 1.0412453378707482 1102 56-57 0.914678009334807 1102 58-59 1.0441162350181585 1102 60-61 0.8920774343831432 1102 62-63 1.1111688768792831 1102 64-65 1.0186329117726238 1102 66-67 1.0413384386053117 1102 68-69 0.8833824283852287 1102 70-71 0.7749865773541948 1102 72-73 0.8454548557934949 1102 74-75 0.9534844035454277 1102 76-77 1.0103614183234093 1102 78-79 0.9396015063544567 1102 80-81 0.7642407120014134 1102 82-83 0.7924636497149464 1102 84-85 0.8808594022018141 1102 86-87 0.8596028427735831 1102 88-89 0.9190493012796885 1102 90-91 0.8630041402043815 1102 92-93 1.0011295186629212 1102 94-95 0.8259957964634879 1102 96-97 0.7562436783939717 1102 98-99 0.8200867006528938 1102 100-101 0.6394120648775754 1102 102-103 0.6306719591325738 1102 104-105 0.6758794722246897 1102 106-107 1.0007281919023612 1102 108-109 1.0870082971382473 1102 110-111 0.9308796897514995 1102 112-113 0.9455917930959359 1102 114-115 1.0288877813380424 1102 116-117 1.0095221315955065 1102 118-119 0.9472047781160811 1102 120-121 0.906786338192564 1102 122-123 0.7987998708154791 1102 124-125 0.645681914372993 1103 1 0.29711970029212864 1103 2 -1.0268888604869169 1103 3 -0.10838274703548478 1103 4 0.21279624494376748 1103 5 0.6240025596260708 1103 6 0.5739870337713242 1103 7 0.5504130109651015 1103 8 0.6405557460868039 1103 9 0.7172582870375024 1103 10-11 0.8565299371058614 1103 12-13 0.8781607662794073 1103 14-15 0.9047734586806371 1103 16-17 0.9782170457404149 1103 18-19 0.9876363029958313 1103 20-21 0.9424027048701049 1103 22-23 0.826649707741403 1103 24-25 0.7440877892271942 1103 26-27 0.7874260794072185 1103 28-29 0.9330206722177294 1103 30-31 0.9087693827250476 1103 32-33 0.8776835994082077 1103 34-35 0.8954521620563511 1103 36-37 0.9489351239722055 1103 38-39 0.9481275588330362 1103 40-41 0.9987657899262103 1103 42-43 0.962740020134703 1103 44-45 0.914183387015413 1103 46-47 0.8515087134791699 1103 48-49 0.8487725003070636 1103 50-51 0.8752487287874899 1103 52-53 0.8851481096826106 1103 54-55 0.8773061209526176 1103 56-57 0.841469528171352 1103 58-59 0.7917747058675317 1103 60-61 0.7059816245992927 1103 62-63 0.7979117991053144 1103 64-65 0.7543183038160386 1103 66-67 0.6787860675150377 1103 68-69 0.6662097943478571 1103 70-71 0.7698621297152961 1103 72-73 0.6693581409572751 1103 74-75 0.5422715981122934 1103 76-77 0.4856081773250054 1103 78-79 0.577032914845141 1103 80-81 0.6515957199676343 1103 82-83 0.6367273220295715 1103 84-85 0.589117828738889 1103 86-87 0.6723915265629827 1103 88-89 0.6347205474721491 1103 90-91 0.684465958454588 1103 92-93 0.6871364687598636 1103 94-95 0.5463449586141955 1103 96-97 0.507271471141685 1103 98-99 0.585537090560031 1103 100-101 0.6037842083060454 1103 102-103 0.679671406110316 1103 104-105 0.5938337130121099 1103 106-107 0.6098157992083664 1103 108-109 0.5819086796697981 1103 110-111 0.6021298197793179 1103 112-113 0.5499030159215899 1103 114-115 0.5027635035433846 1103 116-117 0.5541962216505674 1103 118-119 0.5497370734993154 1103 120-121 0.584894796377089 1103 122-123 0.6167819390246905 1103 124-125 0.49782077644007217 1104 1 0.23673721050485952 1104 2 -0.760933558348313 1104 3 0.057198768908826025 1104 4 0.15715858397155813 1104 5 0.5869950422117469 1104 6 0.6338555889577577 1104 7 0.6687898137196413 1104 8 0.653737177736474 1104 9 0.6510485018917507 1104 10-11 0.7743648212664098 1104 12-13 0.7382374309986162 1104 14-15 0.737192800531389 1104 16-17 0.9013018697924906 1104 18-19 0.8562662752231489 1104 20-21 0.860199867750076 1104 22-23 0.8382880791643785 1104 24-25 0.7618798693002518 1104 26-27 0.6531724439152882 1104 28-29 0.9334822367742319 1104 30-31 0.8738760274385626 1104 32-33 0.9421071776928613 1104 34-35 0.9061631782122461 1104 36-37 0.8912727354298795 1104 38-39 0.8882015507953511 1104 40-41 0.8802732248163068 1104 42-43 0.8645642052722309 1104 44-45 0.827768844705048 1104 46-47 0.8246788887103662 1104 48-49 0.9276086631647615 1104 50-51 0.8906423522063207 1104 52-53 0.7703569131041945 1104 54-55 0.8928059693250674 1104 56-57 0.9450945118235623 1104 58-59 0.9296138618703012 1104 60-61 0.9125183427393715 1104 62-63 0.97347771825671 1104 64-65 0.9943690519309776 1104 66-67 0.8887646740378194 1104 68-69 0.8231477115734904 1104 70-71 0.8469343015758461 1104 72-73 0.8554582916710665 1104 74-75 0.8708511648858135 1104 76-77 0.8139585894606967 1104 78-79 0.8890262546547731 1104 80-81 0.8220761480199315 1104 82-83 0.8710818141172396 1104 84-85 0.9070268419262035 1104 86-87 0.8186746526148809 1104 88-89 0.8536358136541793 1104 90-91 0.8690145395233557 1104 92-93 0.8358050303426374 1104 94-95 0.7941969586987234 1104 96-97 0.8468233046357838 1104 98-99 0.9151262798294866 1104 100-101 0.9128332037092477 1104 102-103 0.9253268626177658 1104 104-105 0.8511104364405924 1104 106-107 0.8440386808629157 1104 108-109 0.7963535600553584 1104 110-111 0.6773417699723794 1104 112-113 0.7738259034584907 1104 114-115 0.8328589746481967 1104 116-117 0.8460910292491945 1104 118-119 0.7657308107958656 1104 120-121 0.7805449130817621 1104 122-123 0.8360926361630732 1104 124-125 0.7961370252600339 1105 1 0.5242330147571757 1105 2 0.23375563734236948 1105 3 0.47354368904824184 1105 4 0.37430751522610706 1105 5 0.6675574973164728 1105 6 0.4795461398110632 1105 7 0.5202992355283413 1105 8 0.6560866841222364 1105 9 0.6003638137130878 1105 10-11 0.7529825273132076 1105 12-13 0.734863479851299 1105 14-15 0.800853966858547 1105 16-17 0.9521438862246043 1105 18-19 0.9288722392977178 1105 20-21 0.9503889138062291 1105 22-23 0.8140148259053106 1105 24-25 0.9538321879727718 1105 26-27 0.8694013292029155 1105 28-29 0.9617495161085117 1105 30-31 0.8814854023043068 1105 32-33 0.8737780823665418 1105 34-35 0.9039210470256975 1105 36-37 0.9433762419770417 1105 38-39 0.9365657384932717 1105 40-41 0.8319741810677073 1105 42-43 0.8445357542139504 1105 44-45 0.983532716758063 1105 46-47 0.9949801665594791 1105 48-49 0.9666940499785852 1105 50-51 0.8706632364579363 1105 52-53 0.8269262058526223 1105 54-55 0.9316692770461543 1105 56-57 0.8990218467806628 1105 58-59 0.8925701201231604 1105 60-61 0.905234828016642 1105 62-63 0.9261576589015803 1105 64-65 0.9085454928575736 1105 66-67 0.8511305371707181 1105 68-69 0.8243802519420989 1105 70-71 0.9551044535280404 1105 72-73 0.7710609077397557 1105 74-75 0.8413581403707404 1105 76-77 0.8095820111589873 1105 78-79 0.783946958463261 1105 80-81 0.6183965706648884 1105 82-83 0.6030159228095826 1105 84-85 0.5588866539443522 1105 86-87 0.6992307224893999 1105 88-89 0.7430470241079412 1105 90-91 0.7293609907702177 1105 92-93 0.5084481489317483 1105 94-95 0.7063603392743367 1105 96-97 0.7371772772008569 1105 98-99 0.7775091253160653 1105 100-101 0.8031266934868952 1105 102-103 0.4441807008796026 1105 104-105 0.4249378214855284 1105 106-107 0.7460293002465335 1105 108-109 0.7487447613766136 1105 110-111 0.6418332071652699 1105 112-113 0.5234701248335831 1105 114-115 0.6369552823396951 1105 116-117 0.45431961420074884 1105 118-119 0.3885332203806975 1105 120-121 0.4563259483190798 1105 122-123 0.5134353571887686 1105 124-125 0.37891749144248976 1106 1 0.47471235125804156 1106 2 0.3628975807253134 1106 3 0.5307197882011536 1106 4 0.4147792908302037 1106 5 0.4913636394657601 1106 6 0.5794858629693209 1106 7 0.6215572447652562 1106 8 0.6740192936168654 1106 9 0.7563349295376156 1106 10-11 0.9360732680375534 1106 12-13 0.7753837702871564 1106 14-15 0.913810305170486 1106 16-17 1.0055310683459098 1106 18-19 0.9623633034911734 1106 20-21 1.0074387858355678 1106 22-23 1.0058555887699114 1106 24-25 1.0289686826391815 1106 26-27 1.0025696715178114 1106 28-29 1.1723765059638467 1106 30-31 1.024045252946145 1106 32-33 0.9680500942071504 1106 34-35 0.9802491855174154 1106 36-37 1.001852278473038 1106 38-39 1.10331708240669 1106 40-41 1.0866629057005142 1106 42-43 0.9994646452529139 1106 44-45 0.8783090005606908 1106 46-47 0.8437276647107623 1106 48-49 0.9756632329589294 1106 50-51 1.0361943202676542 1106 52-53 1.1163792530157934 1106 54-55 1.108755663889525 1106 56-57 0.8758404472509227 1106 58-59 0.8911102194443359 1106 60-61 1.076664573669408 1106 62-63 1.0888209156001167 1106 64-65 0.9690459234855133 1106 66-67 0.8953270230695907 1106 68-69 0.9785344554907383 1106 70-71 1.0792375518794763 1106 72-73 1.1329917925085873 1106 74-75 1.14760606472192 1106 76-77 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28-29 -4.352660759167758 2216 30-31 -3.9963929555816797 2216 32-33 -3.286254273658539 2216 34-35 -2.89855234823694 2216 36-37 -2.9056912138581907 2216 38-39 -3.044721840042609 2216 40-41 -3.3129122227661227 2216 42-43 -3.5073776742073335 2216 44-45 -3.402361673742057 2216 46-47 -3.1682930370505034 2216 48-49 -3.312868422789709 2216 50-51 -3.5215962987257967 2216 52-53 -3.3244446986572136 2216 54-55 -3.108054342778967 2216 56-57 -3.2393235193281384 2216 58-59 -3.185410541156614 2216 60-61 -3.0058995125503394 2216 62-63 -2.8762643529049683 2216 64-65 -2.9169666583997085 2216 66-67 -2.8807914212092314 2216 68-69 -2.8644437640205 2216 70-71 -3.3705074761674396 2216 72-73 -3.712252490235695 2216 74-75 -3.3454072134328676 2216 76-77 -3.60712430869507 2216 78-79 -3.269924249773119 2216 80-81 -2.938363729568188 2216 82-83 -2.7761162218998905 2216 84-85 -2.7678968185502235 2216 86-87 -2.931381936637692 2216 88-89 -2.7234079040930546 2216 90-91 -2.674382701110062 2216 92-93 -2.8133056913183374 2216 94-95 -2.672161943275757 2216 96-97 -2.630023171582554 2216 98-99 -2.6409370658067246 2216 100-101 -2.888682076135183 2216 102-103 -2.608324713747379 2216 104-105 -2.576726575567271 2216 106-107 -2.7996753538687074 2216 108-109 -2.821406311473357 2216 110-111 -2.6216163286088374 2216 112-113 -2.7785055675287573 2216 114-115 -2.8954388915265525 2216 116-117 -2.8615259214582096 2216 118-119 -2.955386314529804 2216 120-121 -2.9183024135921976 2216 122-123 -2.884451383061851 2216 124-125 -3.109012702439948 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93373.0 3 29628.0 4 13847.0 5 10664.0 6 11169.0 7 13423.0 8 15278.0 9 16736.0 10 18213.0 11 19890.0 12 21951.0 13 23668.0 14 25508.0 15 21520.0 16 15158.0 17 13408.0 18 12046.0 19 11354.0 20 11152.0 21 11981.0 22 14295.0 23 18950.0 24 25939.0 25 34478.0 26 41570.0 27 47690.0 28 56052.0 29 65148.0 30 76425.0 31 90348.0 32 109395.0 33 135503.0 34 175328.0 35 237248.0 36 375725.0 37 596407.0 38 239608.0 39 804.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.24715556926443 20.262786116268106 13.560405233170572 24.929653081296884 2 15.69175001873557 20.545572548132228 39.099754000135334 24.66292343299687 3 19.383506368871053 24.929586168789626 28.799438725062526 26.887468737276798 4 13.251367759165323 17.179505916201414 36.44636010102277 33.12276622361049 5 13.339357400511986 38.33873923667675 34.24493105655108 14.076972306260183 6 32.07505261971667 37.54168681180863 16.76196984953851 13.62129071893619 7 28.330362767682622 32.39837157504928 20.6429290419535 18.628336615314595 8 25.539827255278315 37.33725207933462 18.796348804746117 18.326571860640957 9 25.677886773796605 16.704163962971645 19.629643766597052 37.988305496634695 10-11 24.907126413200796 27.369672340749034 27.000305910844997 20.722895335205173 12-13 25.54538556819169 24.14415229968112 28.001215744914614 22.309246387212575 14-15 22.41784605071003 25.717855248505906 25.686971740226618 26.17732696055745 16-17 22.198904435831007 29.097572569942145 26.232818842743516 22.470704151483332 18-19 22.119489253419367 27.691298223292588 28.521106920525284 21.668105602762758 20-21 22.69085113232664 26.834071760465665 28.707167484653546 21.767909622554154 22-23 22.41753159779939 26.61973715400937 28.597771757071783 22.36495949111946 24-25 22.23850186122259 26.8110204734485 28.691909498051533 22.25856816727738 26-27 22.229213924271505 27.037602512650498 28.54439306694585 22.188790496132146 28-29 22.069543062742607 26.943285878344646 28.715478291547665 22.27169276736508 30-31 22.278384058625615 26.82054957332737 28.7170157762272 22.184050591819815 32-33 22.123013276330905 26.85561444837063 28.644919949395803 22.376452325902658 34-35 22.333889518990286 26.886977985226544 28.585834351189437 22.19329814459373 36-37 22.448552269469218 26.762071479451354 28.465947828590508 22.323428422488924 38-39 22.245863141976386 26.979512010702027 28.559533676496248 22.215091170825335 40-41 22.45612313150701 26.815182777874718 28.435137119758043 22.293556970860234 42-43 22.237042312326675 26.73696333445484 28.424199692413453 22.601794660805037 44-45 22.45775897167452 26.871492031640788 28.246979148490663 22.423769848194034 46-47 22.492596456004872 27.190095818481257 27.746209074664698 22.571098650849176 48-49 22.528072436601523 27.11577718285585 27.728891303474583 22.627259077068036 50-51 22.517662142824296 27.272885900258082 27.611924157071066 22.597527799846556 52-53 22.717902979579765 27.277871091538348 27.367897008751218 22.636328920130673 54-55 22.661701950540174 27.22987815894483 27.455533304082074 22.65288658643292 56-57 22.47282995931401 27.480329530351632 27.474567620465724 22.572272889868636 58-59 22.6775068341767 27.148694236026287 27.467319548653524 22.706479381143488 60-61 22.647680015122436 27.231922148548826 27.62694119699878 22.49345663932996 62-63 22.580228872215436 27.214873058803 27.63737422206712 22.56752384691444 64-65 22.450774574542915 27.072230007652138 27.71455523989727 22.762440177907674 66-67 22.417081079509103 27.287122666201363 27.56677863083813 22.729017623451405 68-69 22.364781758131144 27.120042706667757 27.721818479576466 22.793357055624636 70-71 22.38200139591694 26.993525708137035 27.700299540510677 22.92417335543535 72-73 22.63556921685458 27.070093042607223 27.38227081107874 22.912066929459456 74-75 22.353477063746592 27.312892477019396 27.499965464567012 22.833664994667004 76-77 22.562385252231977 27.104983676985157 27.512309285369852 22.820321785413018 78-79 22.459653064796658 27.182649640957674 27.490008122915764 22.867689171329904 80-81 22.32511413798782 27.32451977343623 27.521041777466277 22.829324311109673 82-83 22.497451720833656 27.32362345667344 27.399072150107823 22.779852672385086 84-85 22.508740845650006 27.104764369775758 27.507090463481116 22.87940432109312 86-87 22.425200800475487 27.039658434710535 27.67118706297837 22.863953701835605 88-89 22.420029666875628 27.076722190121377 27.590703264174238 22.912544878828754 90-91 22.402594806025707 27.299027947420463 27.535679491653582 22.76269775490025 92-93 22.41722107100011 27.183135201948595 27.526684116770276 22.87295961028102 94-95 22.31821629640078 27.141394424099474 27.564588329322 22.975800950177742 96-97 22.390863040122618 27.14294958625348 27.47806209142486 22.98812528219905 98-99 22.206007099957596 27.10198241532698 27.44418430371722 23.247826180998207 100-101 22.186308272796325 27.129479794631905 27.528426962350576 23.15578497022119 102-103 22.25247521153262 26.969681657770106 27.49340573693616 23.284437393761117 104-105 22.167370838471864 26.872359355730513 27.66041984937479 23.29984995642284 106-107 22.155416733920514 26.92638648350354 27.659435242179285 23.258761540396662 108-109 22.10384135659322 26.954023469039427 27.40527844355023 23.536856730817117 110-111 22.02383213574055 26.997703086537673 27.57154066601948 23.4069241117023 112-113 22.13473694312807 26.931066172461076 27.592888936066878 23.34130794834398 114-115 21.989305924782315 26.94143196204545 27.48047980474534 23.5887823084269 116-117 21.83121887983586 26.905943626448607 27.6072049553062 23.65563253840933 118-119 21.803966945273284 26.899970627068466 27.559916418014623 23.736146009643626 120-121 21.830452991479405 26.982602336497102 27.558919140592835 23.62802553143066 122-123 21.8364666519319 26.967127418876398 27.497379005287065 23.699026923904633 124-125 21.711976594717523 26.895244784123495 27.4470414769059 23.945737144253084 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 135.0 1 108.5 2 137.5 3 237.0 4 215.5 5 206.5 6 439.0 7 935.0 8 1532.0 9 1834.0 10 1669.0 11 1416.5 12 1447.0 13 1609.0 14 1769.0 15 1961.5 16 2180.5 17 2410.0 18 2623.5 19 2812.0 20 3094.5 21 3666.0 22 4409.5 23 5393.5 24 6697.5 25 8674.0 26 11614.5 27 15702.5 28 19898.0 29 24698.0 30 31019.5 31 36549.0 32 41058.5 33 46481.5 34 52599.5 35 59019.0 36 66667.0 37 73999.5 38 79208.0 39 83212.0 40 87481.0 41 92021.0 42 96171.5 43 100939.5 44 105372.0 45 107128.0 46 106973.5 47 107558.5 48 109224.0 49 108235.5 50 105043.5 51 101534.0 52 97656.0 53 94293.5 54 91425.5 55 85408.0 56 76530.0 57 68990.5 58 62882.5 59 55987.5 60 46285.5 61 36318.0 62 28025.0 63 21288.5 64 15868.0 65 11536.0 66 8522.5 67 6490.0 68 4814.5 69 3356.5 70 2336.5 71 1617.5 72 1193.5 73 978.5 74 757.5 75 521.0 76 330.0 77 182.5 78 112.0 79 69.5 80 37.0 81 24.5 82 22.5 83 16.0 84 14.5 85 8.5 86 7.0 87 5.5 88 3.5 89 4.0 90 2.0 91 0.5 92 1.0 93 1.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.059908102134589664 2 0.07626650380969 3 0.0 4 0.007742976792880824 5 0.029081602977956148 6 3.635200372244518E-4 7 3.6352003722445184E-5 8 0.0 9 5.452800558366777E-4 10-11 0.0622709823765486 12-13 0.011014657127900888 14-15 0.008033792822660386 16-17 0.051947013319374166 18-19 1.817600186122259E-4 20-21 0.011868929215378353 22-23 0.013522945384749607 24-25 0.0 26-27 0.0 28-29 0.05207424533240272 30-31 1.8176001861222592E-5 32-33 0.004362240446693422 34-35 0.0 36-37 1.9993602047344852E-4 38-39 0.0 40-41 0.0 42-43 0.003980544407607748 44-45 0.0 46-47 5.452800558366777E-5 48-49 1.9993602047344852E-4 50-51 3.8169603908567437E-4 52-53 1.9993602047344852E-4 54-55 3.6352003722445184E-5 56-57 0.0020357122084569304 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.452800558366777E-4 66-67 0.0 68-69 7.815680800325713E-4 70-71 0.0 72-73 0.0015813121619263654 74-75 0.002980864305240505 76-77 3.4534403536322925E-4 78-79 7.452160763101262E-4 80-81 0.0015631361600651427 82-83 1.4540801488978073E-4 84-85 7.270400744489037E-5 86-87 5.452800558366777E-4 88-89 1.8176001861222592E-5 90-91 0.0 92-93 0.0065433606700401326 94-95 0.0047802884895015415 96-97 0.005889024603036119 98-99 6.725120688652359E-4 100-101 5.271040539754551E-4 102-103 1.4540801488978073E-4 104-105 4.544000465305648E-4 106-107 0.00392601640202408 108-109 0.003889664398301634 110-111 5.271040539754551E-4 112-113 0.002471936253126272 114-115 3.9987204094689703E-4 116-117 0.0018539521898447043 118-119 0.0017448961786773686 120-121 0.011250945152096783 122-123 5.816320595591229E-4 124-125 0.0017448961786773686 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2750880.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.38738715468835 #Duplication Level Percentage of deduplicated Percentage of total 1 76.98679237787029 43.41084067608582 2 12.520880506984366 14.120394733298363 3 4.316237918569745 7.301441356984152 4 1.9992420340426884 4.509281383579668 5 1.0766725706935592 3.035537654126564 6 0.6795929392286353 2.299228210312659 7 0.4564766426692856 1.8017667624085738 8 0.31958548586235425 1.4416472416271802 9 0.2426060715801153 1.2311930235839397 >10 1.2751913211871913 13.028918235751414 >50 0.08718693426741758 3.3764096218173987 >100 0.038237901659691466 3.478697582965521 >500 8.432419999372762E-4 0.3007241601811657 >1k 3.891886153499649E-4 0.45997409856344923 >5k 6.486476922499415E-5 0.2039452587142011 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5576 0.20269877275635434 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4679 0.170091025417321 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2783 0.10116762635956494 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.6352003722445184E-5 0.0 0.0 0.0 0.0 8 3.6352003722445184E-5 0.0 0.0 0.0 0.0 9 3.6352003722445184E-5 0.0 0.0 0.0 0.0 10-11 3.6352003722445184E-5 0.0 0.0 0.0 0.0 12-13 3.6352003722445184E-5 0.0 0.0 0.0 0.0 14-15 3.6352003722445184E-5 0.0 0.0 0.0 0.0 16-17 3.6352003722445184E-5 0.0 0.0 0.0 0.0 18-19 9.088000930611295E-5 0.0 0.0 0.0 0.0 20-21 1.4540801488978073E-4 0.0 0.0 5.4528005583667775E-5 0.0 22-23 1.4540801488978073E-4 1.8176001861222592E-5 0.0 1.0905601116733555E-4 0.0 24-25 2.5446402605711624E-4 3.6352003722445184E-5 0.0 2.1811202233467107E-4 0.0 26-27 2.5446402605711624E-4 3.6352003722445184E-5 0.0 3.08992031640784E-4 0.0 28-29 3.816960390856744E-4 3.6352003722445184E-5 0.0 7.270400744489036E-4 0.0 30-31 4.3622404466934215E-4 3.6352003722445184E-5 0.0 0.0017812481823998138 0.0 32-33 4.3622404466934215E-4 3.6352003722445184E-5 0.0 0.004471296457860758 0.0 34-35 4.9075205025301E-4 3.6352003722445184E-5 0.0 0.00757939277612982 0.0 36-37 5.816320595591229E-4 3.6352003722445184E-5 0.0 0.013413889373582271 0.0 38-39 6.543360670040133E-4 3.6352003722445184E-5 0.0 0.024083202466119932 0.0 40-41 6.543360670040133E-4 3.6352003722445184E-5 0.0 0.037024515791310414 0.0 42-43 7.08864072587681E-4 3.6352003722445184E-5 0.0 0.048439044960158203 0.0 44-45 7.633920781713488E-4 3.6352003722445184E-5 0.0 0.06321613447333217 0.0 46-47 8.542720874774618E-4 3.6352003722445184E-5 0.0 0.08091956028616297 0.0 48-49 9.088000930611295E-4 3.6352003722445184E-5 0.0 0.10134938637817717 0.0 50-51 9.088000930611295E-4 3.6352003722445184E-5 0.0 0.1251963008201012 0.0 52-53 9.088000930611295E-4 3.6352003722445184E-5 0.0 0.14718926307218055 0.0 54-55 9.088000930611295E-4 3.6352003722445184E-5 0.0 0.1716905135811086 0.0 56-57 9.088000930611295E-4 3.6352003722445184E-5 0.0 0.20102658058512185 0.0 58-59 9.088000930611295E-4 3.6352003722445184E-5 0.0 0.23479759204327344 0.0 60-61 9.088000930611295E-4 3.6352003722445184E-5 0.0 0.27305807596114695 0.0 62-63 9.088000930611295E-4 3.6352003722445184E-5 0.0 0.31380867213400804 0.0 64-65 9.088000930611295E-4 3.6352003722445184E-5 0.0 0.3567949165357994 0.0 66-67 9.451520967835747E-4 3.6352003722445184E-5 0.0 0.4077422497528064 0.0 68-69 9.8150410050602E-4 3.6352003722445184E-5 0.0 0.467650351887396 0.0 70-71 0.001017856104228465 3.6352003722445184E-5 0.0 0.5343381027162217 0.0 72-73 0.0010542081079509102 3.6352003722445184E-5 0.0 0.6028798057348921 0.0 74-75 0.0010542081079509102 3.6352003722445184E-5 0.0 0.6734572209620194 0.0 76-77 0.0010542081079509102 3.6352003722445184E-5 0.0 0.7564851974640843 0.0 78-79 0.0010542081079509102 3.6352003722445184E-5 0.0 0.8521273192578375 0.0 80-81 0.0013268481358692493 3.6352003722445184E-5 0.0 0.9626737625777932 0.0 82-83 0.001417728145175362 3.6352003722445184E-5 0.0 1.0816356947594952 0.0 84-85 0.0014359041470365847 3.6352003722445184E-5 0.0 1.201488251032397 0.0 86-87 0.0014904321526202525 3.6352003722445184E-5 0.0 1.3277569359623103 0.0 88-89 0.0015267841563426977 3.6352003722445184E-5 0.0 1.473819286919095 0.0 90-91 0.0016176641656488106 3.6352003722445184E-5 0.0 1.6445828244052811 0.0 92-93 0.0017085441749549236 3.6352003722445184E-5 0.0 1.8328134996801024 0.0 94-95 0.0017085441749549236 3.6352003722445184E-5 0.0 2.0207715349270052 0.0 96-97 0.0017085441749549236 3.6352003722445184E-5 0.0 2.214800354795556 0.0 98-99 0.0017448961786773686 3.6352003722445184E-5 0.0 2.429349880765428 0.0 100-101 0.0018357761879834815 3.6352003722445184E-5 0.0 2.6799969464316873 0.0 102-103 0.0019266561972895947 3.6352003722445184E-5 0.0 2.9564539347408827 0.0 104-105 0.0022538242307916013 3.6352003722445184E-5 0.0 3.2360735473739313 0.0 106-107 0.002435584249403827 3.6352003722445184E-5 0.0 3.5173108241726285 0.0 108-109 0.0024537602512650497 3.6352003722445184E-5 0.0 3.8174511429069966 0.0 110-111 0.0025082882568487177 3.6352003722445184E-5 0.0 4.1556156575350425 0.0 112-113 0.0025446402605711627 3.6352003722445184E-5 0.0 4.533912784272669 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5215 0.0 49.47767 1 GTATCAA 8080 0.0 43.73398 1 ATCAACG 9890 0.0 35.433582 3 TATCAAC 10225 0.0 34.53557 2 TCAACGC 10135 0.0 34.464 4 CAACGCA 10115 0.0 34.33304 5 AACGCAG 10285 0.0 34.04708 6 GTACATG 9765 0.0 31.183456 1 TACATGG 9850 0.0 30.167515 2 ACATGGG 10155 0.0 28.29849 3 ACGCAGA 12385 0.0 27.793207 7 CGCAGAG 12410 0.0 27.641243 8 GAGTACT 7770 0.0 24.29525 12-13 GCAGAGT 13990 0.0 23.966108 9 CATGGGG 5665 0.0 23.423742 4 AGGACCG 955 0.0 23.078518 5 GTACTTT 8460 0.0 22.137747 14-15 GGACCGA 785 0.0 21.241991 6 GTGGTAT 3115 0.0 21.234426 1 AGTACTT 8550 0.0 20.65103 12-13 >>END_MODULE