FastQCFastQC Report
Thu 26 May 2016
SRR1364612_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364612_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1918462
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78550.4094425638871137No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76800.035.9197431
GTACATG127850.034.8123471
TACATGG133750.031.40492
ACATGGG132400.031.1401143
ATCAACG93900.028.8066223
TCAACGC92550.028.7761824
CAACGCA94500.028.2461745
CATGGGA93600.028.0077934
TATCAAC99500.027.5446222
GTACGCT1954.7255526E-827.4985623
AACGCAG94850.027.4864586
GGTATCA50200.027.298421
ACGACGG1358.2277074E-526.4544946
TGCGCGA600.001289971324.78651854-55
TACCTGG11850.024.6365432
ATGGGAT35100.023.9345575
ACGCAGA108150.023.8285717
GTATCGA1501.6858085E-423.8028269
CATGGGG36000.023.6665844
CGCAGAG105200.022.7965558
Produced by FastQC (version 0.11.5)