##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364612_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1918462 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.019157012231673 31.0 26.0 31.0 7.0 34.0 2 25.498643183967157 30.0 25.0 31.0 7.0 34.0 3 26.236441482812793 31.0 26.0 31.0 7.0 34.0 4 29.45618469378075 35.0 28.0 37.0 7.0 37.0 5 29.869362541452475 35.0 30.0 37.0 7.0 37.0 6 29.792720940002983 35.0 30.0 37.0 7.0 37.0 7 29.752389153394752 35.0 30.0 37.0 7.0 37.0 8 29.485362232871957 35.0 29.0 37.0 2.0 37.0 9 30.264414411127248 37.0 28.0 39.0 2.0 39.0 10-11 30.27086489073018 36.0 28.0 39.0 2.0 39.0 12-13 30.263311444271505 37.0 28.0 39.0 2.0 39.0 14-15 30.801339823254253 37.0 27.5 40.0 2.0 41.0 16-17 31.18985286130244 37.0 29.0 40.0 2.0 41.0 18-19 31.002920568663857 37.0 28.0 40.0 2.0 41.0 20-21 30.905742464536697 37.0 27.5 40.0 2.0 41.0 22-23 30.9934116495401 37.0 28.0 40.0 2.0 41.0 24-25 31.096319603932734 37.0 28.5 40.0 2.0 41.0 26-27 30.90729214339403 37.0 28.0 40.0 2.0 41.0 28-29 30.81372291971381 37.0 28.0 40.0 2.0 41.0 30-31 30.676188008936325 37.0 28.0 40.0 2.0 41.0 32-33 30.58004484842546 37.0 28.0 40.0 2.0 41.0 34-35 30.626308470014003 37.0 28.0 40.0 2.0 41.0 36-37 30.55342143863157 37.0 28.0 40.0 2.0 41.0 38-39 30.237862673328948 37.0 27.0 40.0 2.0 41.0 40-41 30.09253740756919 36.0 27.0 40.0 2.0 41.0 42-43 29.570552869955204 35.5 25.5 40.0 2.0 41.0 44-45 29.42273941313406 35.0 25.5 40.0 2.0 41.0 46-47 29.413310766645367 35.0 26.0 40.0 2.0 41.0 48-49 29.32517349835441 35.0 26.0 40.0 2.0 41.0 50-51 29.095184319522616 35.0 25.5 40.0 2.0 41.0 52-53 28.98225062576168 35.0 25.5 39.5 2.0 41.0 54-55 28.8073876365547 35.0 25.0 39.0 2.0 41.0 56-57 28.578771432532935 35.0 24.5 39.0 2.0 40.5 58-59 28.345678725979457 34.0 24.0 39.0 2.0 40.0 60-61 28.054039642171695 34.0 23.0 39.0 2.0 40.0 62-63 27.842781874230504 34.0 23.0 38.5 2.0 40.0 64-65 27.604793579440198 34.0 22.5 38.0 2.0 40.0 66-67 27.336805732925647 33.5 22.0 38.0 2.0 40.0 68-69 27.088020508094505 33.0 22.0 37.0 2.0 40.0 70-71 26.668975199925775 33.0 20.5 37.0 2.0 39.5 72-73 26.429036384353715 33.0 20.0 36.0 2.0 39.0 74-75 26.385607585659763 33.0 20.5 36.0 2.0 39.0 76-77 26.142026790210075 33.0 20.0 36.0 2.0 39.0 78-79 25.84961964323505 32.5 19.5 35.0 2.0 37.5 80-81 25.468147401408004 32.0 18.0 35.0 2.0 37.0 82-83 25.574802888980862 32.5 19.0 35.0 2.0 37.0 84-85 25.404479473661716 33.0 18.0 35.0 2.0 36.0 86-87 25.277748269186464 33.0 18.0 35.0 2.0 36.0 88-89 25.097622991750683 32.5 16.5 35.0 2.0 36.0 90-91 24.958322604252782 32.0 14.5 35.0 2.0 35.0 92-93 24.83648125425471 32.0 11.0 35.0 2.0 35.0 94-95 24.72559425206233 32.0 9.0 35.0 2.0 35.0 96-97 24.61185965632887 32.0 7.0 35.0 2.0 35.0 98-99 24.511792519215913 32.0 7.0 35.0 2.0 35.0 100-101 24.39728204155203 32.0 3.5 35.0 2.0 35.0 102-103 23.271034557890644 30.0 2.0 34.0 2.0 35.0 104-105 24.102733074723396 32.0 2.0 34.5 2.0 35.0 106-107 24.26274875394978 32.0 2.0 35.0 2.0 35.0 108-109 24.225156922576524 32.0 2.0 35.0 2.0 35.0 110-111 24.144525145663557 32.0 2.0 35.0 2.0 35.0 112-113 24.05089285062722 32.0 2.0 35.0 2.0 35.0 114-115 23.940131469896198 32.0 2.0 35.0 2.0 35.0 116-117 23.829089395567905 32.0 2.0 35.0 2.0 35.0 118-119 23.696412542964104 32.0 2.0 35.0 2.0 35.0 120-121 23.49631293192151 32.0 2.0 35.0 2.0 35.0 122-123 23.279695401837515 32.0 2.0 35.0 2.0 35.0 124-125 22.325214677173697 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.8602816858555293 1101 2 2.147551396697505 1101 3 1.6285140432542633 1101 4 1.7556454732918318 1101 5 1.6106585082924418 1101 6 1.4639856752784617 1101 7 1.553864022148293 1101 8 1.8382274158912253 1101 9 2.093550860484342 1101 10-11 2.0137720693518943 1101 12-13 2.12268396743373 1101 14-15 2.4801925448895794 1101 16-17 2.0524398959597434 1101 18-19 2.0332029738266506 1101 20-21 2.240895325828795 1101 22-23 2.033226952865867 1101 24-25 2.0332700902635708 1101 26-27 2.2484020720024205 1101 28-29 2.181803463678566 1101 30-31 2.377611116025175 1101 32-33 2.3036544964387353 1101 34-35 2.168218915387513 1101 36-37 2.072048098341508 1101 38-39 2.052764487023911 1101 40-41 2.063226251525098 1101 42-43 2.200482121288907 1101 44-45 2.1103580650010727 1101 46-47 1.8864725971275682 1101 48-49 1.7877246547542285 1101 50-51 1.7530647103443044 1101 52-53 1.7492666963559316 1101 54-55 1.5504238258553578 1101 56-57 1.664845630214355 1101 58-59 1.5362559305303272 1101 60-61 1.7519892915352209 1101 62-63 1.6046377944391352 1101 64-65 1.5666593724477096 1101 66-67 1.4701420470364397 1101 68-69 1.3578751807728828 1101 70-71 1.3232435445662745 1101 72-73 1.3082858397451282 1101 74-75 1.2678994908977401 1101 76-77 1.3448068571938876 1101 78-79 1.4036822793788275 1101 80-81 1.4982021223072337 1101 82-83 1.512755302891943 1101 84-85 1.4919806298151386 1101 86-87 1.4763059395863323 1101 88-89 1.398979753556489 1101 90-91 1.388292347660606 1101 92-93 1.3386653391422527 1101 94-95 1.3026685030775909 1101 96-97 1.3330609456731928 1101 98-99 1.3931655411358008 1101 100-101 1.3882298155479589 1101 102-103 1.2327026191141464 1101 104-105 1.3612828036726015 1101 106-107 1.397768999423608 1101 108-109 1.2721879371869065 1101 110-111 1.3772901402132227 1101 112-113 1.1931466727031115 1101 114-115 1.206216854858873 1101 116-117 1.2072596158034408 1101 118-119 1.150300872133247 1101 120-121 1.2063898656721221 1101 122-123 1.1277499820359616 1101 124-125 1.1879412869028165 1102 1 1.484431345719475 1102 2 1.6470587694790204 1102 3 1.4447389130904682 1102 4 1.4105774460809464 1102 5 1.2354181328126401 1102 6 1.127899425135947 1102 7 1.1245838184466983 1102 8 1.0404064718711616 1102 9 1.7083182758303685 1102 10-11 1.677674655526026 1102 12-13 1.7500000754411786 1102 14-15 1.9255933448743754 1102 16-17 1.6874302049324896 1102 18-19 1.5944728309315046 1102 20-21 1.9294741651857663 1102 22-23 1.9214917839379595 1102 24-25 1.6690983880631407 1102 26-27 1.9672449443468878 1102 28-29 1.6766534427763524 1102 30-31 1.7588185990183725 1102 32-33 1.958861285858415 1102 34-35 1.7876330357842818 1102 36-37 1.7983666006027441 1102 38-39 1.9892704441553697 1102 40-41 2.040552573115182 1102 42-43 2.1575195172528794 1102 44-45 1.773795339352155 1102 46-47 1.5833104244372649 1102 48-49 1.5556041249780748 1102 50-51 1.568900038889133 1102 52-53 1.6609092304645472 1102 54-55 1.458150282359746 1102 56-57 1.5014211822228667 1102 58-59 1.5261511344219407 1102 60-61 1.44275740045477 1102 62-63 1.2279361640663176 1102 64-65 1.1732148298261897 1102 66-67 1.161655272438331 1102 68-69 1.0879931357761095 1102 70-71 1.303458884892347 1102 72-73 1.1062459735193038 1102 74-75 1.14273037297124 1102 76-77 0.9487637891286695 1102 78-79 1.0570921908294366 1102 80-81 1.3090259071284294 1102 82-83 1.2812898675557989 1102 84-85 1.2539122320649767 1102 86-87 1.1877145113949439 1102 88-89 1.0195670108057548 1102 90-91 1.176849334701231 1102 92-93 1.3261232971858021 1102 94-95 1.1262162741413206 1102 96-97 0.924885091532655 1102 98-99 1.1449047004866912 1102 100-101 1.2123343456281503 1102 102-103 1.098491630249324 1102 104-105 1.1993229359266202 1102 106-107 1.1268743845944371 1102 108-109 1.1406320047580678 1102 110-111 1.0723192132949677 1102 112-113 1.0109591608268502 1102 114-115 1.0617580797287083 1102 116-117 1.1660848637797265 1102 118-119 1.036102329587738 1102 120-121 1.0415329033341116 1102 122-123 0.9639953740475136 1102 124-125 0.8053661339366016 1103 1 1.348224449167752 1103 2 1.4946637588837604 1103 3 1.2554410899350046 1103 4 1.0483360667706023 1103 5 1.1660326579523037 1103 6 1.0571524592334214 1103 7 1.0907136585545771 1103 8 1.2984526094169127 1103 9 1.8915170814554898 1103 10-11 1.8776590034358733 1103 12-13 1.7998678754797055 1103 14-15 1.9567947029941983 1103 16-17 1.6573695229829681 1103 18-19 1.630902950368995 1103 20-21 1.93566661365427 1103 22-23 1.7973813042615987 1103 24-25 1.6171652345281728 1103 26-27 1.8347661348689428 1103 28-29 1.872012236843009 1103 30-31 1.9923530817769866 1103 32-33 2.075135847599885 1103 34-35 1.7889543129948322 1103 36-37 1.6781236320032455 1103 38-39 1.7289537903314418 1103 40-41 1.813019824126549 1103 42-43 2.1601654428934083 1103 44-45 1.8195476028851907 1103 46-47 1.8011265966584133 1103 48-49 1.514313911146445 1103 50-51 1.4864226742195115 1103 52-53 1.390889966353587 1103 54-55 1.4208918061509266 1103 56-57 1.4209622147792196 1103 58-59 1.48972414540248 1103 60-61 1.596664402959103 1103 62-63 1.3323014116101461 1103 64-65 1.327417054831006 1103 66-67 1.248108268007588 1103 68-69 1.2875045497618558 1103 70-71 1.4178600118428406 1103 72-73 1.2308462432168596 1103 74-75 1.2769858150825826 1103 76-77 1.197952529255815 1103 78-79 1.168667031900526 1103 80-81 1.3312328518404293 1103 82-83 1.3499028515665863 1103 84-85 1.3748612303312058 1103 86-87 1.2804391790890328 1103 88-89 1.157321297070446 1103 90-91 1.2313735111604878 1103 92-93 1.243380328586305 1103 94-95 1.247721282810172 1103 96-97 1.3463884145917966 1103 98-99 1.3475087266858843 1103 100-101 1.151493948787465 1103 102-103 1.120156290237766 1103 104-105 1.2335417762271383 1103 106-107 1.3055018166884444 1103 108-109 1.2978365414562028 1103 110-111 1.279570224660791 1103 112-113 1.3581947609039062 1103 114-115 1.461465506043485 1103 116-117 1.471883120377683 1103 118-119 1.510633970889991 1103 120-121 1.4685948856111324 1103 122-123 1.3589944108419658 1103 124-125 1.3068985939635738 1104 1 1.488499142329644 1104 2 1.8663119178551213 1104 3 1.2441845148327246 1104 4 1.3664249037080651 1104 5 0.9192529969353558 1104 6 0.9896480758319015 1104 7 0.7989602979467456 1104 8 1.1419008852789823 1104 9 1.4692201790592314 1104 10-11 1.516037144873625 1104 12-13 1.5625709730834458 1104 14-15 1.6771716755248782 1104 16-17 1.3288978689794604 1104 18-19 1.2988106067103153 1104 20-21 1.7073498398377964 1104 22-23 1.606358510573692 1104 24-25 1.5897280632832818 1104 26-27 1.6942956497725135 1104 28-29 1.6087860533245966 1104 30-31 1.7336914803743078 1104 32-33 1.7207904355601507 1104 34-35 1.6327415719077507 1104 36-37 1.5020803824415871 1104 38-39 1.505946776889008 1104 40-41 1.7815820684281256 1104 42-43 1.9325091015725455 1104 44-45 1.5666165684024307 1104 46-47 1.373888139615751 1104 48-49 1.3198340747934338 1104 50-51 1.2179597870190406 1104 52-53 1.0659016554243053 1104 54-55 1.1478088137488243 1104 56-57 1.1655034187534987 1104 58-59 1.2191747590786939 1104 60-61 1.177026764151389 1104 62-63 0.9800424986937202 1104 64-65 0.875032484404354 1104 66-67 0.944540179170648 1104 68-69 0.8897459290722018 1104 70-71 0.9070242432864397 1104 72-73 0.8603260795698695 1104 74-75 0.8095223434285828 1104 76-77 0.9649659716871426 1104 78-79 0.9005928063014608 1104 80-81 1.1384742311507736 1104 82-83 1.070405481607498 1104 84-85 1.0754252279933922 1104 86-87 1.0939342112339752 1104 88-89 1.1118449674386497 1104 90-91 1.1221537566309756 1104 92-93 1.1207269095331185 1104 94-95 1.1059182436517858 1104 96-97 1.1805058897525207 1104 98-99 1.1547676396023085 1104 100-101 1.1721310031416436 1104 102-103 0.9549809541769854 1104 104-105 0.9584394968583503 1104 106-107 1.0278600867059815 1104 108-109 0.9957149751207233 1104 110-111 0.9491874075947493 1104 112-113 1.1425840069588453 1104 114-115 1.1347998134660457 1104 116-117 1.0018012968826504 1104 118-119 1.068863076676081 1104 120-121 1.1389835472125327 1104 122-123 1.061723808854822 1104 124-125 0.8101890673709349 1105 1 1.2209223397154716 1105 2 1.6106576398618841 1105 3 1.068848188920537 1105 4 1.2313746442130338 1105 5 0.9899285885727167 1105 6 0.9368621507519066 1105 7 0.965737697569093 1105 8 0.9128281701355512 1105 9 1.0822440028513611 1105 10-11 1.3045177138405037 1105 12-13 1.2697042031464285 1105 14-15 1.5714576967141518 1105 16-17 1.233659348383803 1105 18-19 1.146894300924533 1105 20-21 1.1678588797627114 1105 22-23 1.2552019203044722 1105 24-25 0.9382387087983801 1105 26-27 1.2614571472680183 1105 28-29 1.4384986300706721 1105 30-31 1.4510717677975613 1105 32-33 1.4522855566012929 1105 34-35 1.2353245368222119 1105 36-37 0.8148274115344272 1105 38-39 1.2630543401232543 1105 40-41 1.2277586155047082 1105 42-43 1.5527108179710467 1105 44-45 1.2906058946199117 1105 46-47 0.9207887852439072 1105 48-49 0.9628721453715556 1105 50-51 1.0183598277241472 1105 52-53 0.8112827569496162 1105 54-55 0.823258096321382 1105 56-57 0.840514329982458 1105 58-59 0.8979774637066384 1105 60-61 0.8573232329837168 1105 62-63 0.7005599057786718 1105 64-65 0.5454999146685999 1105 66-67 0.4651227144201364 1105 68-69 0.24163385435572593 1105 70-71 0.5336797537397366 1105 72-73 0.49277833587633424 1105 74-75 0.5280952903649592 1105 76-77 0.5914120770002853 1105 78-79 0.5267106701881161 1105 80-81 0.7090946447598476 1105 82-83 0.6105186644073299 1105 84-85 0.5621064052754221 1105 86-87 0.683283834146426 1105 88-89 0.6977535392548901 1105 90-91 0.46553341086373834 1105 92-93 0.6048005857692544 1105 94-95 0.6115754614580098 1105 96-97 0.6501666805622115 1105 98-99 0.7424690450590532 1105 100-101 0.7261715499468586 1105 102-103 0.686966232498122 1105 104-105 0.6762097617807292 1105 106-107 0.6354135969557504 1105 108-109 0.6814433137871809 1105 110-111 0.7010600684647095 1105 112-113 0.688834727778378 1105 114-115 0.7778494171566415 1105 116-117 0.7426192433102123 1105 118-119 0.7347903049977873 1105 120-121 0.6721596759311126 1105 122-123 0.6718434366292705 1105 124-125 0.5192820219960552 1106 1 0.6683795012528755 1106 2 -2.084395958515767 1106 3 -0.055941772970530934 1106 4 0.6701736574468491 1106 5 0.8356305309998291 1106 6 0.8399106215281478 1106 7 1.0262916372621333 1106 8 1.0679294662028767 1106 9 1.4647535788598276 1106 10-11 1.7286459850198526 1106 12-13 1.6413114184141229 1106 14-15 1.7275870959303354 1106 16-17 1.452772910521496 1106 18-19 1.568877073975191 1106 20-21 1.6117599428620508 1106 22-23 1.3184356126009433 1106 24-25 1.5058530217412454 1106 26-27 1.3756416118861665 1106 28-29 1.5929635209218027 1106 30-31 1.691042759869152 1106 32-33 1.7784806981058416 1106 34-35 1.5999931505052984 1106 36-37 1.3715852013182896 1106 38-39 1.7943814728012732 1106 40-41 1.6129878510801738 1106 42-43 1.7846161138689922 1106 44-45 1.5866930057570343 1106 46-47 1.426167966800861 1106 48-49 1.3903126391005145 1106 50-51 1.3487573941975057 1106 52-53 1.1980053443575045 1106 54-55 1.1303079829080112 1106 56-57 1.2209570578362516 1106 58-59 1.2506669491750735 1106 60-61 1.3679207488639165 1106 62-63 1.221142531927356 1106 64-65 1.0964316203299127 1106 66-67 1.0178735125039822 1106 68-69 0.9989782321629299 1106 70-71 1.0507464101192774 1106 72-73 0.8482921812647852 1106 74-75 0.926484789423931 1106 76-77 0.8329387201085439 1106 78-79 0.9144711712067419 1106 80-81 1.1197005521876662 1106 82-83 1.098188798324017 1106 84-85 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2216 104-105 -3.5081957730984215 2216 106-107 -3.5191897212374315 2216 108-109 -3.5499761116787134 2216 110-111 -3.49585068679821 2216 112-113 -3.6289354585787237 2216 114-115 -3.64561738669115 2216 116-117 -3.422877150945144 2216 118-119 -3.4889946366461793 2216 120-121 -3.5808301008485195 2216 122-123 -3.2414445544173667 2216 124-125 -3.167400273805235 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 188667.0 3 26170.0 4 16675.0 5 13827.0 6 13364.0 7 14478.0 8 15142.0 9 16042.0 10 17032.0 11 18057.0 12 18286.0 13 18383.0 14 17928.0 15 15455.0 16 13170.0 17 11449.0 18 10259.0 19 9419.0 20 9653.0 21 10167.0 22 12457.0 23 18103.0 24 27184.0 25 33411.0 26 36773.0 27 39516.0 28 44986.0 29 50714.0 30 58769.0 31 67836.0 32 82209.0 33 102185.0 34 131619.0 35 177034.0 36 243390.0 37 255426.0 38 63189.0 39 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.016069802469794 20.778731583256143 13.780773810176864 22.4244248040972 2 13.203943778576402 23.252958774719175 40.26003349201272 23.283063954691706 3 18.187927184491407 29.424127850729253 28.02476689519566 24.36317806958368 4 11.468462434189439 18.80515368128682 37.86055612113379 31.86582776338995 5 12.285249655693313 41.972881238907775 32.57959852768027 13.162270577718637 6 29.08279591076338 39.510579224909584 17.758068654568408 13.648556209758627 7 27.323915107427236 35.33611278462248 21.00206551653328 16.337906591417003 8 24.748436708232553 38.24007564399262 19.646646432246907 17.364841215527914 9 25.839115503040926 19.36545367351054 20.10183793068501 34.69359289276352 10-11 22.761772205295483 30.806225606177996 26.78564343306304 19.646358755463485 12-13 23.96759903095806 28.22567042025066 26.859005859894165 20.947724688897118 14-15 21.83111392001755 29.034679257996025 24.723628851763735 24.41057797022269 16-17 21.089245087846724 32.044173219424394 25.62653484392697 21.240046848801914 18-19 21.33320797783297 30.369615915970027 27.578915204838722 20.718260901358278 20-21 22.369912655761542 29.146187676124107 27.61101090503798 20.872888763076368 22-23 22.23096676149253 28.97552280692223 27.237861248802055 21.555649182783192 24-25 21.867668264113956 29.220748863160605 27.50659015565382 21.404992717071618 26-27 21.572614625068297 29.079270661935 27.64210498260115 21.706009730395557 28-29 21.608228363727516 28.556485137139397 28.13255937832926 21.70272712080383 30-31 21.642348228615944 28.81667033888482 27.784054776267936 21.7569266562313 32-33 21.483369079096512 28.780023045831538 27.64643139581895 22.090176479253007 34-35 22.073092892637064 28.678576882763952 27.67032883839498 21.578001386204008 36-37 22.277951960402966 28.23959233434014 27.325164008677515 22.157291696579378 38-39 21.891331620890686 28.680715799633766 27.506405990972592 21.921546588502956 40-41 21.85060832106104 28.51426938522194 28.107337623021174 21.527784670695844 42-43 21.6411057978193 28.396676803887257 27.732900138139776 22.229317260153667 44-45 21.736815650845664 28.661518476692798 27.620635407623062 21.981030464838472 46-47 22.254345219944778 28.77964168904892 26.964564684358045 22.00144840664826 48-49 21.710140031480126 28.97565837926687 26.930912111755756 22.383289477497243 50-51 22.105531114969388 28.826703914990276 26.98186755087921 22.085897419161128 52-53 22.24366544327142 28.816624943787712 26.939931067153378 21.99977854578749 54-55 22.02546122522518 28.91268217596733 27.10859339198824 21.953263206819255 56-57 22.075448641489963 29.188173536679873 26.61264969512641 22.12372812670375 58-59 21.767319015285278 28.567356395769533 27.07895008870034 22.58637450024485 60-61 21.702899997989718 29.10568927395751 27.062279244542157 22.129131483510616 62-63 21.89109324423097 28.88970972120706 27.31669625143573 21.90250078312624 64-65 21.667429735459624 28.899024855508532 27.03534461561432 22.398200793417526 66-67 21.5657851775893 29.19351096395702 26.735706480136155 22.504997378317523 68-69 21.914009371580043 28.84823735420036 27.040920860965954 22.196832413253638 70-71 21.96240850345001 28.566420935809646 27.14851804560968 22.322652515130667 72-73 21.866865189954012 28.82355642674783 26.665007087135233 22.644571296162926 74-75 21.718123117108817 29.232726328575847 26.695466448167572 22.353684106147767 76-77 21.87781886593891 28.886146004727344 27.264723876420916 21.971311252912827 78-79 21.905580523894866 28.749248905137303 27.10279154096214 22.242379030005687 80-81 21.752381638573603 29.21268679217503 26.870871632859185 22.164059936392178 82-83 22.035898723787735 29.084826894490977 26.794166065796226 22.08510831592506 84-85 21.720222357894727 29.087256706326947 26.921859163414574 22.270661772363752 86-87 21.787359569043936 29.020970256887995 27.073396421301744 22.11827375276632 88-89 21.97984781165949 28.921058498992497 26.913202100957275 22.185891588390742 90-91 21.7857602486421 29.092623932741745 27.008920238899826 22.11269557971633 92-93 21.840322700223147 29.02419833992747 27.054702616457256 22.080776343392134 94-95 21.55278659759358 28.649654286430483 27.391507561831553 22.406051554144383 96-97 22.10655533126494 28.441854369368002 27.10161061083745 22.349979688529604 98-99 21.649861597377466 28.68937689711319 26.963384067473335 22.69737743803601 100-101 21.41780682476627 28.80295144779633 27.310079502210716 22.469162225226682 102-103 21.598389218143595 28.651669990257705 27.208525591999148 22.54141519959955 104-105 21.193899663861906 29.06507465428984 27.227890353053315 22.51313532879494 106-107 21.385190741903706 28.94828197341928 27.109118348881996 22.557408935795017 108-109 21.483106563858357 29.07837206309361 26.593153346565067 22.845368026482966 110-111 21.29619069715125 29.085971400542466 26.949741776018328 22.668096126287956 112-113 21.09338120226057 29.17272400134547 27.412893765896833 22.321001030497133 114-115 21.07029895734038 29.552173296985373 26.97703341066886 22.400494335005387 116-117 20.916022446623508 29.535331720260626 27.113556700062418 22.435089133053445 118-119 20.811457877277583 29.33901116763666 27.09684758610289 22.75268336898287 120-121 20.62176998496997 29.426873464157122 27.27659560035721 22.674760950515694 122-123 20.805569495648733 29.290427944767483 27.261315810831938 22.64268674875185 124-125 20.672734290127458 29.27417378712553 27.301956950788263 22.75113497195875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8281.0 1 6282.5 2 3401.5 3 2047.0 4 1394.5 5 1137.5 6 1051.5 7 1086.5 8 1132.5 9 1126.5 10 1099.0 11 1057.0 12 1072.5 13 1128.0 14 1209.5 15 1332.5 16 1498.5 17 1640.5 18 1826.0 19 2139.0 20 2574.5 21 3137.5 22 3773.0 23 4709.5 24 5888.5 25 7476.5 26 9798.0 27 12679.5 28 15432.0 29 18799.0 30 23327.5 31 28365.5 32 35821.5 33 43317.5 34 49163.5 35 55295.5 36 64392.0 37 71642.0 38 73667.0 39 75461.5 40 73545.5 41 69045.5 42 65194.5 43 63731.5 44 64085.5 45 65015.0 46 63470.0 47 60159.5 48 59436.5 49 58251.5 50 57223.0 51 54774.0 52 51689.0 53 51212.5 54 51398.0 55 49879.5 56 47136.0 57 44288.0 58 41782.5 59 37875.0 60 32280.5 61 27393.0 62 22614.0 63 16492.5 64 11144.0 65 8384.0 66 6404.5 67 4808.5 68 3846.0 69 3193.0 70 2500.5 71 1846.0 72 1506.0 73 1288.0 74 1000.0 75 778.5 76 593.0 77 391.0 78 280.5 79 231.5 80 188.0 81 142.0 82 104.5 83 75.5 84 59.0 85 48.5 86 35.0 87 23.0 88 17.5 89 14.5 90 17.5 91 16.0 92 6.5 93 4.5 94 4.5 95 4.0 96 2.0 97 1.0 98 1.0 99 1.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22804725868951276 2 0.2694867034113785 3 0.32484354654926706 4 0.31092614813324426 5 0.34657970812035893 6 0.25296305061033264 7 0.24264228324564155 8 0.22059337114834696 9 0.22121887220075248 10-11 0.15731351467998844 12-13 0.18665993905534745 14-15 0.20417396852270203 16-17 0.17540092011204808 18-19 0.20365271764569742 20-21 0.19789289545479657 22-23 0.22160981035850594 24-25 0.21128904299381485 26-27 0.2592441236782381 28-29 0.18879706765106632 30-31 0.165757778887463 32-33 0.2093343522050476 34-35 0.16468921458960356 36-37 0.165080152747357 38-39 0.2005773374713703 40-41 0.22200074851625937 42-43 0.28900754875520074 44-45 0.18501799879278297 46-47 0.16961503537729702 48-49 0.1712569756398615 50-51 0.17654767204145821 52-53 0.2004209622082689 54-55 0.18710300230080137 56-57 0.18608656309064242 58-59 0.1576262652061912 60-61 0.17229947739387072 62-63 0.15960701853880868 64-65 0.15382113380405762 66-67 0.1411286749489956 68-69 0.14472530600032735 70-71 0.14678424696449552 72-73 0.12061745293886457 74-75 0.1229891444292355 76-77 0.14584599538588724 78-79 0.1524658815238457 80-81 0.16322971213399068 82-83 0.16601840432596526 84-85 0.15124094196288485 86-87 0.18056130379439364 88-89 0.17383716748103428 90-91 0.1615095842398755 92-93 0.16283877397623722 94-95 0.18671206414304792 96-97 0.17138728835911266 98-99 0.16766034458852977 100-101 0.14553324485968447 102-103 0.18845825458101334 104-105 0.14237967705380664 106-107 0.14923412608641712 108-109 0.1624999609061842 110-111 0.13482153933723995 112-113 0.12491777267415252 114-115 0.12262426881533227 116-117 0.12770646486612713 118-119 0.11055731101267578 120-121 0.10245185987525424 122-123 0.13164190898751188 124-125 0.12747190197147507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1918462.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.18266767906974 #Duplication Level Percentage of deduplicated Percentage of total 1 77.82266500486159 36.718809408239544 2 9.703984386991117 9.15719740988566 3 4.202056310653464 5.947926794229005 4 2.228739878546845 4.206315721302642 5 1.4358358025397524 3.3873281756471765 6 0.914656069320695 2.5893548015641517 7 0.6736565963676953 2.2249440721361124 8 0.4744065060040725 1.7907011614063002 9 0.37385447870766203 1.5875506466275922 >10 1.9146419291791505 16.175124255977217 >50 0.15427452824994214 5.06608363763633 >100 0.09404952263118019 8.288255107463964 >500 0.006102138054850556 1.8805940279513438 >1k 9.571981262510678E-4 0.5363689919418142 >5k 1.1964976578138347E-4 0.4434457879910484 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7855 0.4094425638871137 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.212508770046005E-5 0.0 16-17 0.0 0.0 0.0 1.5637526310138017E-4 2.6062543850230027E-5 18-19 0.0 5.212508770046005E-5 0.0 2.085003508018402E-4 5.212508770046005E-5 20-21 0.0 5.212508770046005E-5 0.0 2.606254385023003E-4 5.212508770046005E-5 22-23 0.0 5.212508770046005E-5 0.0 4.430632454539105E-4 5.212508770046005E-5 24-25 0.0 5.212508770046005E-5 0.0 0.001016439210158971 5.212508770046005E-5 26-27 0.0 5.212508770046005E-5 0.0 0.0019546907887672523 5.212508770046005E-5 28-29 0.0 5.212508770046005E-5 0.0 0.003753006314433124 5.212508770046005E-5 30-31 0.0 5.212508770046005E-5 0.0 0.00883520236522798 5.212508770046005E-5 32-33 0.0 5.212508770046005E-5 0.0 0.016601840432596528 5.212508770046005E-5 34-35 0.0 5.212508770046005E-5 0.0 0.02634923183258256 5.212508770046005E-5 36-37 0.0 5.212508770046005E-5 0.0 0.04120488182721367 5.212508770046005E-5 38-39 0.0 5.212508770046005E-5 0.0 0.06880511576460727 5.212508770046005E-5 40-41 0.0 5.212508770046005E-5 0.0 0.10417198776936942 5.212508770046005E-5 42-43 0.0 1.3031271925115014E-4 0.0 0.13367478740782981 5.212508770046005E-5 44-45 0.0 1.5637526310138017E-4 0.0 0.1678427823954814 5.212508770046005E-5 46-47 0.0 1.5637526310138017E-4 0.0 0.20938647729274806 5.212508770046005E-5 48-49 0.0 1.5637526310138017E-4 0.0 0.2588792480643349 5.212508770046005E-5 50-51 0.0 2.085003508018402E-4 0.0 0.314757342079228 5.212508770046005E-5 52-53 0.0 2.085003508018402E-4 0.0 0.368680745305354 5.212508770046005E-5 54-55 0.0 2.085003508018402E-4 0.0 0.42221321037372644 5.212508770046005E-5 56-57 0.0 2.085003508018402E-4 0.0 0.4854148792105343 5.212508770046005E-5 58-59 0.0 2.085003508018402E-4 0.0 0.5587288150612313 5.212508770046005E-5 60-61 0.0 2.085003508018402E-4 0.0 0.6443963966969375 5.212508770046005E-5 62-63 0.0 2.085003508018402E-4 0.0 0.7270407232460169 5.212508770046005E-5 64-65 0.0 2.085003508018402E-4 0.0 0.808746798216488 5.212508770046005E-5 66-67 0.0 2.085003508018402E-4 0.0 0.9003566398500465 5.212508770046005E-5 68-69 0.0 2.085003508018402E-4 0.0 1.0147451448087061 5.212508770046005E-5 70-71 0.0 2.085003508018402E-4 0.0 1.1445626757267018 5.212508770046005E-5 72-73 0.0 2.085003508018402E-4 0.0 1.280739467344154 5.212508770046005E-5 74-75 0.0 2.085003508018402E-4 0.0 1.4140493791380804 5.212508770046005E-5 76-77 0.0 2.085003508018402E-4 0.0 1.5610942515410782 5.212508770046005E-5 78-79 0.0 2.085003508018402E-4 0.0 1.736677609460078 5.212508770046005E-5 80-81 0.0 2.085003508018402E-4 0.0 1.9413988914036349 5.212508770046005E-5 82-83 0.0 2.085003508018402E-4 0.0 2.1554245015017237 5.212508770046005E-5 84-85 0.0 2.085003508018402E-4 0.0 2.3695543617752137 5.212508770046005E-5 86-87 0.0 2.085003508018402E-4 0.0 2.599347810902692 5.212508770046005E-5 88-89 0.0 2.606254385023003E-4 0.0 2.8507992339697115 5.212508770046005E-5 90-91 0.0 3.3881307005299036E-4 0.0 3.1468697321083243 5.212508770046005E-5 92-93 0.0 3.648756139032204E-4 0.0 3.473146718569354 5.212508770046005E-5 94-95 0.0 4.691257893041405E-4 0.0 3.793689945383333 5.212508770046005E-5 96-97 0.0 4.691257893041405E-4 0.0 4.127499007017079 5.212508770046005E-5 98-99 0.0 4.691257893041405E-4 0.0 4.488048238641161 5.212508770046005E-5 100-101 0.0 6.255010524055207E-4 0.0 4.909818385769434 5.212508770046005E-5 102-103 0.0 6.255010524055207E-4 0.0 5.360804644553815 5.212508770046005E-5 104-105 0.0 6.255010524055207E-4 0.0 5.819870291931766 5.212508770046005E-5 106-107 0.0 6.515635962557507E-4 0.0 6.272237865540209 5.212508770046005E-5 108-109 0.0 6.776261401059807E-4 0.0 6.7629434411523395 5.212508770046005E-5 110-111 0.0 8.86126490907821E-4 0.0 7.3156518085841675 5.212508770046005E-5 112-113 0.0 8.86126490907821E-4 0.0 7.911050622842673 5.212508770046005E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7680 0.0 35.919743 1 GTACATG 12785 0.0 34.812347 1 TACATGG 13375 0.0 31.4049 2 ACATGGG 13240 0.0 31.140114 3 ATCAACG 9390 0.0 28.806622 3 TCAACGC 9255 0.0 28.776182 4 CAACGCA 9450 0.0 28.246174 5 CATGGGA 9360 0.0 28.007793 4 TATCAAC 9950 0.0 27.544622 2 GTACGCT 195 4.7255526E-8 27.498562 3 AACGCAG 9485 0.0 27.486458 6 GGTATCA 5020 0.0 27.29842 1 ACGACGG 135 8.2277074E-5 26.454494 6 TGCGCGA 60 0.0012899713 24.786518 54-55 TACCTGG 1185 0.0 24.636543 2 ATGGGAT 3510 0.0 23.934557 5 ACGCAGA 10815 0.0 23.828571 7 GTATCGA 150 1.6858085E-4 23.802826 9 CATGGGG 3600 0.0 23.666584 4 CGCAGAG 10520 0.0 22.796555 8 >>END_MODULE