FastQCFastQC Report
Thu 26 May 2016
SRR1364612_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364612_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1918462
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119210.6213831704771844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106140.5532556808526831No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64050.33386118672144666No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29850.15559338678587326No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29840.1555412616981728No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82650.051.5884551
GTATCAA139250.045.5018271
TCAACGC174950.035.8483544
ATCAACG176750.035.6469573
TATCAAC176750.035.597272
CAACGCA176600.035.146665
AACGCAG180500.034.478166
GTACATG136750.030.0907551
GTTCGAC902.177876E-829.78281216-17
ACGCAGA209250.029.542577
CGCAGAG210400.029.2678748
TACATGG145200.027.7948422
ACATGGG141150.027.145073
ACGTACC557.765478E-427.03894232-33
GTAAGGT7950.026.9449834
GTATAGG9950.026.93221
GAGTACT126750.026.82970212-13
GCAGAGT226000.026.4841069
GTACTTT130900.025.77436414-15
ATGGGAT33850.024.98835
Produced by FastQC (version 0.11.5)