##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364612_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1918462 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.940626397603914 31.0 30.0 33.0 26.0 34.0 2 29.934296848204447 31.0 30.0 34.0 26.0 34.0 3 29.951676916196412 31.0 30.0 34.0 26.0 34.0 4 33.72542067552029 35.0 35.0 37.0 28.0 37.0 5 33.24437648491344 35.0 33.0 37.0 27.0 37.0 6 33.296557867708614 35.0 33.0 37.0 27.0 37.0 7 33.09954119497806 35.0 33.0 37.0 26.0 37.0 8 33.02845456412481 35.0 33.0 37.0 26.0 37.0 9 34.32296860714468 38.0 34.0 39.0 26.0 39.0 10-11 34.13998713552836 38.0 34.0 39.0 25.5 39.0 12-13 34.05378683549635 38.0 34.0 39.0 25.0 39.0 14-15 35.02572842203807 39.0 34.0 41.0 25.0 41.0 16-17 34.953551594975565 39.0 34.0 41.0 25.0 41.0 18-19 35.04997779471264 39.0 34.0 41.0 25.0 41.0 20-21 35.09375635274506 39.0 34.0 41.0 25.0 41.0 22-23 35.081142863397865 39.0 34.0 41.0 25.0 41.0 24-25 35.06263871788964 39.0 34.0 41.0 25.0 41.0 26-27 34.93814446155305 39.0 34.0 41.0 24.5 41.0 28-29 34.684782393396375 39.0 34.0 41.0 24.0 41.0 30-31 34.55095983136492 38.5 34.0 41.0 24.0 41.0 32-33 34.30374669917882 38.0 33.0 41.0 22.5 41.0 34-35 34.132453496603006 38.0 33.0 41.0 21.5 41.0 36-37 33.92933193360098 38.0 33.0 40.0 19.0 41.0 38-39 33.77306092067499 38.0 33.0 40.0 18.0 41.0 40-41 33.5459438341755 38.0 33.0 40.0 15.5 41.0 42-43 33.3170756053547 38.0 33.0 40.0 14.0 41.0 44-45 33.046962097763725 38.0 32.0 40.0 9.0 41.0 46-47 32.674151481759864 38.0 31.5 40.0 4.5 41.0 48-49 32.31417849298032 38.0 31.0 40.0 2.0 41.0 50-51 32.096321949561684 38.0 31.0 40.0 2.0 41.0 52-53 31.924802524105246 37.0 30.5 40.0 2.0 41.0 54-55 31.714063922037546 37.0 30.0 40.0 2.0 41.0 56-57 31.501378969195116 37.0 30.0 40.0 2.0 41.0 58-59 31.296956885254957 36.5 30.0 40.0 2.0 41.0 60-61 31.090230611813006 36.0 29.5 40.0 2.0 41.0 62-63 30.81197907490479 36.0 29.0 39.5 2.0 41.0 64-65 30.538050010894146 35.0 29.0 39.0 2.0 41.0 66-67 30.26061840161546 35.0 29.0 39.0 2.0 41.0 68-69 29.94860935478524 35.0 29.0 38.5 2.0 40.0 70-71 29.682328083642002 35.0 28.5 38.0 2.0 40.0 72-73 29.513556953434573 35.0 29.0 37.0 2.0 40.0 74-75 29.23287143555619 35.0 28.5 37.0 2.0 39.0 76-77 28.93857345102483 34.0 28.0 36.0 2.0 39.0 78-79 28.634873403799503 34.0 28.0 36.0 2.0 39.0 80-81 28.374114003821813 34.0 28.0 35.5 2.0 37.0 82-83 28.114326736729733 34.0 27.0 35.0 2.0 37.0 84-85 28.081271872989927 34.0 28.0 35.0 2.0 36.5 86-87 27.941711902555276 34.0 28.0 35.0 2.0 36.0 88-89 27.794316488937493 34.0 27.0 35.0 2.0 36.0 90-91 27.63863448950253 34.0 27.0 35.0 2.0 36.0 92-93 27.507107776958833 34.0 27.0 35.0 2.0 35.5 94-95 27.414943585017582 34.0 27.0 35.0 2.0 35.0 96-97 27.333287550131303 34.0 27.0 35.0 2.0 35.0 98-99 27.240920591598893 34.0 26.0 35.0 2.0 35.0 100-101 27.147402189879184 34.0 26.0 35.0 2.0 35.0 102-103 25.956971782605024 32.0 24.5 34.0 2.0 35.0 104-105 26.888193250635144 33.5 25.0 35.0 2.0 35.0 106-107 27.056743891721595 34.0 26.0 35.0 2.0 35.0 108-109 27.07369444899091 34.0 26.0 35.0 2.0 35.0 110-111 27.00506864352799 34.0 26.0 35.0 2.0 35.0 112-113 26.950550493051203 34.0 25.0 35.0 2.0 35.0 114-115 26.871420439914893 34.0 25.0 35.0 2.0 35.0 116-117 26.752086046009772 34.0 25.0 35.0 2.0 35.0 118-119 26.64947051335914 34.0 25.0 35.0 2.0 35.0 120-121 26.50762720345777 34.0 25.0 35.0 2.0 35.0 122-123 26.32430170626262 34.0 25.0 35.0 2.0 35.0 124-125 25.533615208432586 33.0 21.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6955524681379792 1101 2 0.6335338724433406 1101 3 0.5446903705631279 1101 4 0.6339882649223938 1101 5 0.8684586147610389 1101 6 0.8436777000969613 1101 7 0.8184693668181424 1101 8 0.9687931691868812 1101 9 1.0443935393230817 1101 10-11 1.0286182068907124 1101 12-13 1.0715607293852827 1101 14-15 1.2301624784910672 1101 16-17 1.2650370534685607 1101 18-19 1.2342730544120997 1101 20-21 1.2743330982380172 1101 22-23 1.281274464225632 1101 24-25 1.1238641526381485 1101 26-27 1.1628298374282267 1101 28-29 1.1891434323802343 1101 30-31 1.2547679343631373 1101 32-33 1.2920568593219315 1101 34-35 1.141381377765235 1101 36-37 1.1968239402267002 1101 38-39 1.1024370344061794 1101 40-41 1.3220924907954128 1101 42-43 1.1669535385105405 1101 44-45 1.1867414495417918 1101 46-47 1.2198336689401827 1101 48-49 1.274774963788893 1101 50-51 1.1695323210105073 1101 52-53 1.2350200680392298 1101 54-55 1.2097633652982047 1101 56-57 1.1908177656661927 1101 58-59 1.2106886701728783 1101 60-61 1.0990048358231235 1101 62-63 1.0142463039212863 1101 64-65 0.9503255352554838 1101 66-67 0.9998861252378504 1101 68-69 1.0493304880988994 1101 70-71 1.0301694130783545 1101 72-73 1.060637158572547 1101 74-75 1.006617876695195 1101 76-77 1.0854808016140325 1101 78-79 0.894032207930195 1101 80-81 0.9454046219197068 1101 82-83 0.9709122312280591 1101 84-85 1.1008397788799513 1101 86-87 1.0847358687241275 1101 88-89 1.059805289057497 1101 90-91 1.0200560098895544 1101 92-93 1.0209409275440535 1101 94-95 0.9760671567790311 1101 96-97 0.9728249963012239 1101 98-99 1.0856099787788303 1101 100-101 1.1018871413132487 1101 102-103 1.0229437241665842 1101 104-105 1.0635017972678185 1101 106-107 1.0409549715447284 1101 108-109 0.9456715110083884 1101 110-111 0.9724801905992564 1101 112-113 0.9530952116948654 1101 114-115 1.0009601536269095 1101 116-117 1.046799862992966 1101 118-119 1.0008514388086418 1101 120-121 0.9577409211411592 1101 122-123 1.0262693582918878 1101 124-125 0.8332535112500707 1102 1 0.5356015883447185 1102 2 -0.21282344143192944 1102 3 0.280110125057103 1102 4 0.4199538522746522 1102 5 0.8117276262762587 1102 6 0.8266124661821692 1102 7 0.746940559764596 1102 8 0.7490771542893029 1102 9 1.0397356705889962 1102 10-11 1.0880401181132981 1102 12-13 1.2516473310650582 1102 14-15 1.2809099702251956 1102 16-17 1.4434113361425247 1102 18-19 1.3950046794643498 1102 20-21 1.3501979274099156 1102 22-23 1.34313385882146 1102 24-25 1.4092193002837945 1102 26-27 1.3459251705335262 1102 28-29 1.4998871712165567 1102 30-31 1.3214348385549783 1102 32-33 1.3706038228000565 1102 34-35 1.3751543702024378 1102 36-37 1.3098548089988 1102 38-39 1.2503508507707082 1102 40-41 1.2379325698437924 1102 42-43 1.0972463120445397 1102 44-45 0.8478785300723288 1102 46-47 0.8233195884476459 1102 48-49 1.0319273219388592 1102 50-51 1.0027518797837445 1102 52-53 1.069773089359483 1102 54-55 1.1637518880816096 1102 56-57 1.1834095928998813 1102 58-59 1.2829397912917173 1102 60-61 0.9455866109912847 1102 62-63 1.1687497812898897 1102 64-65 1.0699935700671723 1102 66-67 1.0817976328576577 1102 68-69 0.896609874713878 1102 70-71 0.7195527502120882 1102 72-73 0.7995941380123703 1102 74-75 0.8642127442511587 1102 76-77 0.960896233149775 1102 78-79 0.9236641864199129 1102 80-81 0.8558410031761206 1102 82-83 0.7740744039183625 1102 84-85 0.9227156102180736 1102 86-87 0.8366937887636539 1102 88-89 0.9432322073108281 1102 90-91 0.797084237381668 1102 92-93 0.9542839994096752 1102 94-95 0.7959777047971208 1102 96-97 0.8402526936371402 1102 98-99 0.8008524444801957 1102 100-101 0.6520522213117275 1102 102-103 0.6302540365596982 1102 104-105 0.671605681279523 1102 106-107 0.8313166748852794 1102 108-109 0.9303713266888458 1102 110-111 0.8064899956385858 1102 112-113 0.9764826532636697 1102 114-115 1.0791422784321405 1102 116-117 1.0269209058235091 1102 118-119 0.9882013228963551 1102 120-121 0.9098456317404882 1102 122-123 0.8736404296254499 1102 124-125 0.8011921252431549 1103 1 0.21700449722547432 1103 2 -0.9450445934257665 1103 3 -0.03218037773619287 1103 4 0.19008725624306066 1103 5 0.5456859194760284 1103 6 0.48464415564469476 1103 7 0.4460086583968419 1103 8 0.5368391461983748 1103 9 0.4921720990228238 1103 10-11 0.7722905033955172 1103 12-13 0.6907291553763741 1103 14-15 0.7465945685684829 1103 16-17 0.8803208911415652 1103 18-19 0.9728820152378006 1103 20-21 0.9573779023665736 1103 22-23 0.8048071876598328 1103 24-25 0.7815242511603131 1103 26-27 0.7350231285377689 1103 28-29 0.8266334628751935 1103 30-31 0.7885762852897145 1103 32-33 0.835379636708744 1103 34-35 0.8591446899866781 1103 36-37 0.8783158473343775 1103 38-39 0.9166793520228822 1103 40-41 0.9077668984895197 1103 42-43 0.8676901090008045 1103 44-45 0.8993088903112039 1103 46-47 0.8830884191161061 1103 48-49 0.826205880983359 1103 50-51 0.8951023421224065 1103 52-53 0.8248898780321916 1103 54-55 0.8517185611696512 1103 56-57 0.8517584177891102 1103 58-59 0.914804316431379 1103 60-61 0.9759576859286767 1103 62-63 0.9073040097622425 1103 64-65 0.7505214067075094 1103 66-67 0.7513733408137355 1103 68-69 0.7734789750799003 1103 70-71 0.7327908064632922 1103 72-73 0.6834180640381824 1103 74-75 0.6417996735518692 1103 76-77 0.43939002047399356 1103 78-79 0.5336721328656822 1103 80-81 0.6327621166417323 1103 82-83 0.6645340937661786 1103 84-85 0.6824736048241213 1103 86-87 0.6505357748934912 1103 88-89 0.5681691173474306 1103 90-91 0.5618634225097736 1103 92-93 0.6017384879475287 1103 94-95 0.5353364507035003 1103 96-97 0.4902411351705638 1103 98-99 0.6495309264682518 1103 100-101 0.7048404605719867 1103 102-103 0.6372153638569458 1103 104-105 0.6714164113893304 1103 106-107 0.7391393487438833 1103 108-109 0.7013212881606243 1103 110-111 0.6929165030552724 1103 112-113 0.709580081504857 1103 114-115 0.6801731491699528 1103 116-117 0.7740987135676818 1103 118-119 0.8552380210277342 1103 120-121 0.710894803383713 1103 122-123 0.6459010730467547 1103 124-125 0.6015704242221531 1104 1 0.3723990033622364 1104 2 -0.607323962215947 1104 3 0.17459928328075947 1104 4 0.25655715688596104 1104 5 0.5932078364836215 1104 6 0.5500503508521817 1104 7 0.6632266595657512 1104 8 0.6888496663620245 1104 9 0.7228208424477174 1104 10-11 0.9094529814785091 1104 12-13 0.7969769636756112 1104 14-15 0.9400223885567911 1104 16-17 1.0677639069218117 1104 18-19 0.9087674623447555 1104 20-21 0.920449205697949 1104 22-23 0.9738808288053562 1104 24-25 0.9863459928318505 1104 26-27 0.8718670794436676 1104 28-29 1.0590823231908004 1104 30-31 1.1019252040506657 1104 32-33 0.9463673631844927 1104 34-35 1.0332446315413293 1104 36-37 1.170694573225667 1104 38-39 1.0999961258393611 1104 40-41 0.9979860685655026 1104 42-43 0.993636923729035 1104 44-45 0.9932714852849074 1104 46-47 1.0353473320325293 1104 48-49 1.090609738376692 1104 50-51 1.0380362988728393 1104 52-53 0.7803486740579011 1104 54-55 0.9944947154653825 1104 56-57 0.9802476053986986 1104 58-59 0.8861602486347735 1104 60-61 0.7911096438480243 1104 62-63 0.8170965287569842 1104 64-65 0.7488790922715083 1104 66-67 0.8388426570030987 1104 68-69 0.7541160294340763 1104 70-71 0.7955844943651016 1104 72-73 0.7968604953648928 1104 74-75 0.9236494689931334 1104 76-77 0.7797319257925182 1104 78-79 0.7141630738358771 1104 80-81 0.6391472715219173 1104 82-83 0.6551068582314059 1104 84-85 0.6670907877580774 1104 86-87 0.7199630104282662 1104 88-89 0.7247412973591238 1104 90-91 0.7219423237371245 1104 92-93 0.8034567813432041 1104 94-95 0.6586619913230187 1104 96-97 0.6902820469180782 1104 98-99 0.7679090679060074 1104 100-101 0.7510765798005075 1104 102-103 0.8477413720392946 1104 104-105 0.7674041378650749 1104 106-107 0.7701299974873059 1104 108-109 0.6806813115387662 1104 110-111 0.7115255036397272 1104 112-113 0.7805268962330878 1104 114-115 0.8932298411629418 1104 116-117 0.9016586200551124 1104 118-119 0.7760445669073412 1104 120-121 0.8076014077086668 1104 122-123 0.842461563987726 1104 124-125 0.6298959648416762 1105 1 0.42185140665934995 1105 2 0.19821781139583905 1105 3 0.37035464561270715 1105 4 0.3818714342007823 1105 5 0.4829546733579235 1105 6 0.5098004667486506 1105 7 0.5376145565818433 1105 8 0.5081416237121203 1105 9 0.637085719597458 1105 10-11 0.7953236749351689 1105 12-13 0.865447243297055 1105 14-15 0.7883689698934973 1105 16-17 0.8888384085669685 1105 18-19 0.8253668902119244 1105 20-21 1.0586715234012871 1105 22-23 0.8802720952994108 1105 24-25 0.8194159490738642 1105 26-27 0.8428255715457524 1105 28-29 1.0989681227633952 1105 30-31 0.9543212647012709 1105 32-33 0.9463106021799774 1105 34-35 0.7946436433118791 1105 36-37 0.7978001012047713 1105 38-39 0.9004810819117708 1105 40-41 0.8934864586560707 1105 42-43 0.9177049464786649 1105 44-45 0.9382520938261933 1105 46-47 0.938092099730774 1105 48-49 0.8523043144217439 1105 50-51 0.8091264041407626 1105 52-53 0.8261049409476975 1105 54-55 0.6619159341309349 1105 56-57 0.8670167509307483 1105 58-59 0.7124627553906855 1105 60-61 0.8081820423962078 1105 62-63 0.7756096618061363 1105 64-65 0.8215234540494158 1105 66-67 0.8304552574938207 1105 68-69 0.7413520398817219 1105 70-71 0.8126083859990771 1105 72-73 0.6434527998390607 1105 74-75 0.6812044491493943 1105 76-77 0.7631781780963109 1105 78-79 0.7652260423685711 1105 80-81 0.6496530324246699 1105 82-83 0.7762391385086502 1105 84-85 0.49592142060935984 1105 86-87 0.5145345326860422 1105 88-89 0.5881759034807175 1105 90-91 0.5971656929889448 1105 92-93 0.4794286722083001 1105 94-95 0.5510163292432146 1105 96-97 0.6449698048584054 1105 98-99 0.5126738263325308 1105 100-101 0.4988622222061778 1105 102-103 0.29341996002450443 1105 104-105 0.28197045891526784 1105 106-107 0.6001893590956122 1105 108-109 0.6328688716070623 1105 110-111 0.5283725542158138 1105 112-113 0.29527537261847314 1105 114-115 0.44440056514842396 1105 116-117 0.2981785599020199 1105 118-119 0.22341795707705714 1105 120-121 0.2806271536862148 1105 122-123 0.34142901121242986 1105 124-125 0.284671917171476 1106 1 0.44547311436257075 1106 2 0.4653181115627163 1106 3 0.5217910413399132 1106 4 0.3802128564539231 1106 5 0.44079839813201005 1106 6 0.4570759407491565 1106 7 0.42436324979506423 1106 8 0.49459908477345493 1106 9 0.49035158355772523 1106 10-11 0.6286154939411261 1106 12-13 0.5997918523429462 1106 14-15 0.7316076328239944 1106 16-17 0.785975937496751 1106 18-19 0.6643440658675246 1106 20-21 0.82805306079932 1106 22-23 0.7115345343553798 1106 24-25 0.7742190403559448 1106 26-27 0.7827045669810602 1106 28-29 0.9050311433968474 1106 30-31 0.8418687068755304 1106 32-33 0.7925853758132746 1106 34-35 0.7363386827211187 1106 36-37 0.7913858327803354 1106 38-39 0.8319037363411965 1106 40-41 0.9528880293498148 1106 42-43 0.9280004996678812 1106 44-45 0.8336569954211654 1106 46-47 0.8054869132887603 1106 48-49 0.9049068470506754 1106 50-51 1.033556405220466 1106 52-53 0.998783369970166 1106 54-55 1.110762578542822 1106 56-57 0.8760601677117528 1106 58-59 0.8186893280631509 1106 60-61 0.9048019004116696 1106 62-63 0.9644511316150748 1106 64-65 0.8141034192904506 1106 66-67 0.7119865586315477 1106 68-69 0.753654081943214 1106 70-71 0.8035240091540707 1106 72-73 0.9357039649561152 1106 74-75 0.9258362420074242 1106 76-77 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86-87 -3.1268848528629363 2216 88-89 -3.022479143039952 2216 90-91 -2.914618278674041 2216 92-93 -2.9827685143478817 2216 94-95 -2.8205733318771635 2216 96-97 -2.7811595818232178 2216 98-99 -2.7986119822568902 2216 100-101 -2.9336009806371024 2216 102-103 -2.8620867278047797 2216 104-105 -2.891852229159504 2216 106-107 -3.0157631607343625 2216 108-109 -2.952465754368969 2216 110-111 -2.956796441519561 2216 112-113 -3.0482377298587195 2216 114-115 -3.06515891180031 2216 116-117 -3.106067252439594 2216 118-119 -3.2986267817989727 2216 120-121 -3.1723459827303344 2216 122-123 -3.0775834393716828 2216 124-125 -3.1716579821431026 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57670.0 3 18606.0 4 9153.0 5 7455.0 6 8995.0 7 11020.0 8 12522.0 9 13179.0 10 14879.0 11 16783.0 12 20564.0 13 24749.0 14 28404.0 15 22122.0 16 12821.0 17 10849.0 18 9782.0 19 9199.0 20 8926.0 21 9334.0 22 10628.0 23 13654.0 24 18589.0 25 24311.0 26 28875.0 27 32950.0 28 38473.0 29 44891.0 30 52510.0 31 61811.0 32 75084.0 33 93050.0 34 120102.0 35 166690.0 36 259090.0 37 393897.0 38 156536.0 39 309.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.19490289591118 21.25738790105376 13.847529160434021 22.700180042601044 2 15.658283163248008 21.64092564394289 38.986029580918725 23.714761611890374 3 18.612305065203273 28.111685297910515 29.613096324034565 23.66291331285165 4 12.370327353708403 18.13357924653807 37.66522510650099 31.83086829325254 5 12.008359626632544 40.238576819451986 33.879952382665664 13.873111171249807 6 29.590112877289275 39.10104745745926 17.197067431865744 14.11177223338572 7 26.896284627998885 34.69967088219626 21.440247448216333 16.96379704158852 8 26.501801964281807 35.40904120071182 19.419253547894094 18.66990328711228 9 27.034523236763903 15.9878276543475 20.64722142441256 36.330427684476035 10-11 24.153154898804196 28.240731444045984 26.768126301833068 20.83798735531675 12-13 25.785792052897623 25.67691799622733 27.986210502527925 20.551079448347117 14-15 23.158017926158074 26.31584297461208 26.746455481219446 23.7796836180104 16-17 20.79963868981249 29.589416448583705 27.837129836982765 21.773815024621037 18-19 20.710706573042813 27.043608483275882 31.654345132572686 20.591339811108615 20-21 21.626947407930643 26.100466309223748 32.032185501927536 20.240400780918076 22-23 21.406990562248314 25.98669951342417 31.851701633901147 20.754608290426365 24-25 20.996115638464563 26.517700116030447 31.95398188757453 20.53220235793047 26-27 20.609112924832495 26.514989611470018 32.07949909875724 20.79639836494025 28-29 20.647968169545464 25.90062944317045 32.530253065464905 20.921149321819186 30-31 20.72613374672003 26.224001309382206 32.13845257294645 20.911412370951314 32-33 20.674128487042246 26.165900745834524 32.02642009854107 21.133550668582163 34-35 21.12165891219112 26.255119986739377 31.923332336006656 20.699888765062845 36-37 21.367707815339195 25.738040655568167 31.61859681056348 21.275654718529154 38-39 21.16038785235256 26.277325274099773 31.572321995431757 20.989964878115906 40-41 21.023820122577355 26.115450814245992 31.905870431627005 20.954858631549648 42-43 21.08176507153776 26.05207224277618 31.23849785692969 21.62766482875637 44-45 21.166616800332765 26.31326552206924 31.050523805006303 21.46959387259169 46-47 21.789126338996116 26.557686979905515 30.125311349745616 21.527875331352753 48-49 21.456612929918958 27.05681517573363 29.482018757256807 22.004553137090603 50-51 21.922345264036892 26.963932146387705 29.154368349298014 21.95935424027739 52-53 21.954463985296535 27.066742873644188 29.06803609875528 21.910757042303995 54-55 21.758028045382186 27.241300583488233 29.106753222112296 21.893918149017285 56-57 21.80221754131371 27.40640658955157 28.704967757506004 22.08640811162872 58-59 21.49924939795461 26.77165539026094 29.30621435943413 22.42288085235032 60-61 21.601574594649257 27.27843970847481 29.25004508820086 21.869940608675073 62-63 21.930483898039157 26.940043639123424 29.619142834207818 21.510329628629602 64-65 21.64808091117332 26.85877319157997 29.51823855670947 21.974907340537243 66-67 21.5973320288158 27.24427881403927 29.111347816987728 22.047041340157204 68-69 21.91604880611828 26.99601448671009 29.2926365478762 21.79530015929543 70-71 21.980445794600048 26.728024844901803 29.205660576023917 22.08586878447423 72-73 21.852161423168553 27.09675418383897 28.77453805168671 22.276546341305764 74-75 21.6796359833901 27.47756693950189 28.720624862513777 22.122172214594237 76-77 21.760131398290905 27.139757157109777 29.312848655315094 21.78726278928422 78-79 21.782052548438458 27.013205263965844 29.07753932297578 22.127202864619917 80-81 21.65456304464996 27.482441407940016 28.700857053200686 22.16213849420934 82-83 21.987476400317338 27.24247807353614 28.667007565451 22.103037960695517 84-85 21.608570619887505 27.30660148942303 28.674729379434872 22.410098511254596 86-87 21.623164246882844 27.256912001914042 28.8247870097654 22.295136741437712 88-89 21.81857133474627 27.121855945022627 28.652300644995837 22.407272075235266 90-91 21.58270531290169 27.38024000475381 28.79421119626034 22.242843486084166 92-93 21.65890161560847 27.30431745527005 28.757778626322285 22.279002302799196 94-95 21.362409694576225 26.894817476051045 28.99464175221323 22.7481310771595 96-97 21.951446461933678 26.727278224461198 28.722392740374993 22.598882573230135 98-99 21.530912552047386 26.85952782567539 28.658511793933002 22.95104782834422 100-101 21.423563673438963 26.921404902804497 28.8613794552173 22.793651968539237 102-103 21.668792069887324 26.609474945913636 28.770036584040476 22.951696400158564 104-105 21.42021460559686 26.847800789126847 28.744749340548186 22.987235264728103 106-107 21.61513875252396 26.725150603783487 28.606104740946897 23.053605902745648 108-109 21.690644650604657 26.771111946316562 28.13511770749657 23.403125695582208 110-111 21.38287591402199 26.67156299584455 28.5162871577174 23.429273932416063 112-113 21.297304566895313 26.67764112984149 28.866113460589148 23.15894084267405 114-115 21.184178085155068 27.07666020486298 28.42579411640525 23.3133675935767 116-117 21.031846871465994 26.993201938371502 28.63046541818778 23.34448577197472 118-119 20.853873335797175 26.75263538417993 28.57052008146325 23.822971198559642 120-121 20.643840658286553 27.047553112514283 28.580090001418 23.72851622778116 122-123 20.80338065816915 26.982193372437962 28.482211877027357 23.732214092365528 124-125 20.634030354579835 26.908133779497824 28.501523775617038 23.956312090305303 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 145.0 1 122.0 2 305.0 3 662.0 4 585.0 5 529.0 6 1423.0 7 3094.5 8 4808.0 9 5672.0 10 4565.5 11 2814.5 12 2259.0 13 2339.0 14 2488.5 15 2617.0 16 2807.5 17 3093.0 18 3414.0 19 3684.0 20 3942.5 21 4330.5 22 4887.0 23 5775.0 24 6763.5 25 8142.0 26 10273.5 27 12855.5 28 15452.0 29 18908.0 30 23605.5 31 28388.0 32 35868.0 33 43591.0 34 48543.0 35 53812.5 36 63911.0 37 71381.5 38 72898.5 39 76094.0 40 74180.0 41 68540.5 42 63935.0 43 62133.5 44 63340.0 45 64616.0 46 63187.5 47 60328.0 48 60153.5 49 58863.0 50 57496.5 51 54631.0 52 50916.5 53 50336.0 54 51070.0 55 49676.0 56 46323.5 57 42736.5 58 40704.0 59 37993.5 60 32113.0 61 27265.5 62 22484.0 63 15727.0 64 10130.5 65 7358.0 66 5600.0 67 4185.5 68 3359.0 69 2808.5 70 2097.5 71 1522.5 72 1282.0 73 1136.5 74 885.5 75 700.0 76 537.0 77 355.0 78 268.5 79 204.5 80 147.5 81 101.5 82 70.5 83 56.0 84 45.0 85 31.0 86 18.5 87 8.5 88 3.5 89 4.5 90 8.0 91 6.0 92 1.0 93 0.5 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.059318349803123546 2 0.07730150505978227 3 0.0 4 0.00886126490907821 5 0.035080184022409616 6 3.6487561390322043E-4 7 0.0 8 0.0 9 7.818763155069008E-4 10-11 0.06325379392450828 12-13 0.009929829206937642 14-15 0.007714512979668089 16-17 0.05191658734965822 18-19 2.606254385023003E-4 20-21 0.010789893153995232 22-23 0.013630710433670305 24-25 0.0 26-27 0.0 28-29 0.05488771734858444 30-31 0.0 32-33 0.0038572564898340443 34-35 0.0 36-37 2.085003508018402E-4 38-39 0.0 40-41 0.0 42-43 0.0036226935951819738 44-45 0.0 46-47 2.6062543850230027E-5 48-49 2.3456289465207026E-4 50-51 4.430632454539105E-4 52-53 1.3031271925115014E-4 54-55 0.0 56-57 0.0016158777187142619 58-59 1.042501754009201E-4 60-61 0.0 62-63 0.0 64-65 6.515635962557507E-4 66-67 2.6062543850230027E-5 68-69 7.297512278064409E-4 70-71 0.0 72-73 0.002006815876467712 74-75 0.004587007717640485 76-77 0.0010425017540092011 78-79 0.0011728144732603514 80-81 0.0014595024556128817 82-83 2.085003508018402E-4 84-85 5.212508770046005E-5 86-87 2.866879823525303E-4 88-89 0.0 90-91 0.0 92-93 0.007532075172716478 94-95 0.005785884734751066 96-97 0.006567761050257967 98-99 9.38251578608281E-4 100-101 4.691257893041405E-4 102-103 1.3031271925115014E-4 104-105 6.515635962557507E-4 106-107 0.005238571313896236 108-109 0.0054731342085483055 110-111 9.38251578608281E-4 112-113 0.003596631051331744 114-115 4.691257893041405E-4 116-117 0.0024759416657718527 118-119 0.002658379472723463 120-121 0.014073773679124216 122-123 9.90376666308741E-4 124-125 0.0019025657010667922 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1918462.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.83725872771908 #Duplication Level Percentage of deduplicated Percentage of total 1 71.33320981296633 25.563866959459443 2 11.736478973472906 8.412064670495669 3 5.207561284477726 5.598741632768441 4 2.9362584631218343 4.209098169374078 5 1.831783001354591 3.2823040676291138 6 1.267844488916275 2.7261642585483186 7 0.9497018692734421 2.3824298122345553 8 0.6802946931462073 1.9503917543499911 9 0.5470172867029215 1.7643240028896734 >10 3.067360619745915 20.21760409680884 >50 0.25023859614009697 6.2825266485765825 >100 0.1778952999417325 12.01792314714706 >500 0.011543686986979114 2.8621797380387908 >1k 0.0023679357922008434 1.1954698655683194 >5k 1.479959870125527E-4 0.33970649906677397 >10k+ 2.959919740251054E-4 1.1952046770444578 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11921 0.6213831704771844 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10614 0.5532556808526831 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6405 0.33386118672144666 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2985 0.15559338678587326 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2984 0.1555412616981728 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.6062543850230027E-5 0.0 12-13 0.0 0.0 0.0 5.212508770046005E-5 0.0 14-15 0.0 0.0 0.0 1.042501754009201E-4 0.0 16-17 2.6062543850230027E-5 0.0 0.0 2.085003508018402E-4 0.0 18-19 5.212508770046005E-5 0.0 0.0 2.606254385023003E-4 0.0 20-21 5.212508770046005E-5 0.0 0.0 2.606254385023003E-4 0.0 22-23 5.212508770046005E-5 0.0 0.0 5.212508770046006E-4 0.0 24-25 5.212508770046005E-5 2.6062543850230027E-5 0.0 0.0012510021048110413 0.0 26-27 5.212508770046005E-5 5.212508770046005E-5 0.0 0.002241378771119782 0.0 28-29 5.212508770046005E-5 5.212508770046005E-5 0.0 0.004117881928336345 0.0 30-31 5.212508770046005E-5 5.212508770046005E-5 0.0 0.0094607034176335 0.0 32-33 5.212508770046005E-5 5.212508770046005E-5 0.0 0.017748592362006647 0.0 34-35 5.212508770046005E-5 5.212508770046005E-5 0.0 0.028356047709050272 0.0 36-37 5.212508770046005E-5 5.212508770046005E-5 0.0 0.0444366372646422 0.0 38-39 5.212508770046005E-5 5.212508770046005E-5 0.0 0.07362668637689983 0.0 40-41 5.212508770046005E-5 5.212508770046005E-5 0.0 0.11081793645117807 0.0 42-43 5.212508770046005E-5 5.212508770046005E-5 0.0 0.14164992582600022 0.0 44-45 5.212508770046005E-5 5.212508770046005E-5 0.0 0.17745986107621625 0.0 46-47 5.212508770046005E-5 5.212508770046005E-5 0.0 0.2205412460606465 0.0 48-49 5.212508770046005E-5 5.212508770046005E-5 0.0 0.27125895639319414 0.0 50-51 5.212508770046005E-5 7.818763155069008E-5 0.0 0.3287268655829514 0.0 52-53 5.212508770046005E-5 1.042501754009201E-4 0.0 0.383301832405333 0.0 54-55 5.212508770046005E-5 1.042501754009201E-4 0.0 0.43847623773627 0.0 56-57 5.212508770046005E-5 1.042501754009201E-4 0.0 0.5040756606072989 0.0 58-59 5.212508770046005E-5 1.042501754009201E-4 0.0 0.5799176632114684 0.0 60-61 5.212508770046005E-5 1.042501754009201E-4 0.0 0.6668101844081353 0.0 62-63 5.212508770046005E-5 1.042501754009201E-4 0.0 0.7516698271844843 0.0 64-65 5.212508770046005E-5 1.042501754009201E-4 0.0 0.835148155136771 0.0 66-67 5.212508770046005E-5 1.042501754009201E-4 0.0 0.929755189313106 0.0 68-69 5.212508770046005E-5 1.042501754009201E-4 0.0 1.0474275747968944 0.0 70-71 5.212508770046005E-5 1.042501754009201E-4 0.0 1.1828485526426897 0.0 72-73 5.212508770046005E-5 1.042501754009201E-4 0.0 1.3215794735574642 0.0 74-75 5.212508770046005E-5 1.042501754009201E-4 0.0 1.4574956397364138 0.0 76-77 5.212508770046005E-5 1.042501754009201E-4 0.0 1.6077983301206906 0.0 78-79 5.212508770046005E-5 1.042501754009201E-4 0.0 1.7870304441787224 0.0 80-81 5.212508770046005E-5 1.042501754009201E-4 0.0 1.9950616691912586 0.0 82-83 5.212508770046005E-5 1.042501754009201E-4 0.0 2.213205161217684 0.0 84-85 5.212508770046005E-5 1.5637526310138017E-4 0.0 2.431687466314162 0.0 86-87 5.212508770046005E-5 1.5637526310138017E-4 0.0 2.6663024860539326 0.0 88-89 5.212508770046005E-5 1.824378069516102E-4 0.0 2.9230185429786983 0.0 90-91 5.212508770046005E-5 2.3456289465207026E-4 0.0 3.224927050939763 0.0 92-93 5.212508770046005E-5 2.606254385023003E-4 0.0 3.558371236959606 0.0 94-95 5.212508770046005E-5 2.606254385023003E-4 0.0 3.8870720399987073 0.0 96-97 5.212508770046005E-5 3.1275052620276033E-4 0.0 4.227735550665064 0.0 98-99 5.212508770046005E-5 3.1275052620276033E-4 0.0 4.596390233426567 0.0 100-101 5.212508770046005E-5 3.1275052620276033E-4 0.0 5.029627899848942 0.0 102-103 5.212508770046005E-5 3.648756139032204E-4 0.0 5.493150242225283 0.0 104-105 5.212508770046005E-5 4.691257893041405E-4 0.0 5.964048284511239 0.0 106-107 5.212508770046005E-5 4.951883331543705E-4 0.0 6.427857314869932 0.0 108-109 5.212508770046005E-5 5.212508770046006E-4 0.0 6.929743721793812 0.0 110-111 5.212508770046005E-5 5.733759647050606E-4 0.0 7.498871491851285 2.6062543850230027E-5 112-113 5.212508770046005E-5 5.733759647050606E-4 0.0 8.111706147945593 5.212508770046005E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8265 0.0 51.588455 1 GTATCAA 13925 0.0 45.501827 1 TCAACGC 17495 0.0 35.848354 4 ATCAACG 17675 0.0 35.646957 3 TATCAAC 17675 0.0 35.59727 2 CAACGCA 17660 0.0 35.14666 5 AACGCAG 18050 0.0 34.47816 6 GTACATG 13675 0.0 30.090755 1 GTTCGAC 90 2.177876E-8 29.782812 16-17 ACGCAGA 20925 0.0 29.54257 7 CGCAGAG 21040 0.0 29.267874 8 TACATGG 14520 0.0 27.794842 2 ACATGGG 14115 0.0 27.14507 3 ACGTACC 55 7.765478E-4 27.038942 32-33 GTAAGGT 795 0.0 26.944983 4 GTATAGG 995 0.0 26.9322 1 GAGTACT 12675 0.0 26.829702 12-13 GCAGAGT 22600 0.0 26.484106 9 GTACTTT 13090 0.0 25.774364 14-15 ATGGGAT 3385 0.0 24.9883 5 >>END_MODULE