FastQCFastQC Report
Thu 26 May 2016
SRR1364611_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364611_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3768744
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82500.043.5388531
ATCAACG97750.036.683943
TCAACGC100650.035.5682684
CAACGCA101300.035.0469635
AACGCAG101900.034.168926
TATCAAC107100.033.4813732
GTACATG126500.033.103841
GGTATCA52800.032.942951
TACATGG129500.031.2317452
ACATGGG133350.029.1686573
ACGCAGA115950.028.5859857
CGCAGAG116200.027.1370948
GCAGAGT134000.021.9782969
AGTACAT97000.020.6330682
CATGGGG67200.020.4759834
CATGGGA78500.019.880794
CGCATAG4900.019.4275288
AGAGTAC126750.019.36196310-11
GAGTACA115800.019.18731
TACCTGG37250.018.3894122
Produced by FastQC (version 0.11.5)