##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364611_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3768744 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.104197313481627 30.0 22.0 31.0 2.0 31.0 2 24.013268611505584 30.0 16.0 31.0 2.0 34.0 3 24.58576358595861 30.0 23.0 31.0 2.0 34.0 4 27.653573445158386 35.0 25.0 35.0 2.0 37.0 5 27.848164799731688 35.0 26.0 35.0 2.0 37.0 6 27.863901872878603 35.0 26.0 36.0 2.0 37.0 7 27.58926528307574 35.0 25.0 36.0 2.0 37.0 8 27.362294440800436 35.0 25.0 36.0 2.0 37.0 9 28.26241474613293 35.0 23.0 39.0 2.0 39.0 10-11 28.14329150507437 35.0 22.5 39.0 2.0 39.0 12-13 28.137030798589663 35.0 23.0 39.0 2.0 39.0 14-15 28.598429078759395 36.0 20.5 40.0 2.0 41.0 16-17 28.944203161583808 36.0 24.5 40.0 2.0 41.0 18-19 28.74509412154288 36.0 22.0 40.0 2.0 41.0 20-21 28.625554561413566 36.0 20.5 40.0 2.0 41.0 22-23 28.657548111519382 36.0 22.0 40.0 2.0 41.0 24-25 28.708441592212154 36.0 22.5 40.0 2.0 41.0 26-27 28.483154069366346 36.0 20.5 40.0 2.0 41.0 28-29 28.346816339873442 35.0 20.0 40.0 2.0 41.0 30-31 28.191081697244492 35.0 19.0 40.0 2.0 41.0 32-33 28.083504875894995 35.0 19.0 40.0 2.0 41.0 34-35 28.115660018297874 35.0 19.0 40.0 2.0 41.0 36-37 28.06370981950485 35.0 19.0 40.0 2.0 41.0 38-39 27.772490251394096 35.0 16.5 40.0 2.0 41.0 40-41 27.624068522563483 35.0 16.0 40.0 2.0 41.0 42-43 27.12823423400475 34.0 14.5 39.5 2.0 41.0 44-45 26.95949976968454 34.0 14.0 39.0 2.0 40.5 46-47 26.90329032696304 34.0 13.5 39.0 2.0 40.0 48-49 26.837051415537907 34.0 9.0 39.0 2.0 40.0 50-51 26.61180541846302 33.0 9.0 39.0 2.0 40.0 52-53 26.50581055651432 33.0 8.5 39.0 2.0 40.0 54-55 26.33299356496488 33.0 7.5 39.0 2.0 40.0 56-57 26.11246187058606 33.0 7.0 38.5 2.0 40.0 58-59 25.89584779438455 33.0 3.5 38.0 2.0 40.0 60-61 25.615418691213836 33.0 2.0 38.0 2.0 40.0 62-63 25.435775685480362 32.0 2.0 38.0 2.0 40.0 64-65 25.2200747782285 32.0 2.0 38.0 2.0 40.0 66-67 24.977867560120824 32.0 2.0 37.0 2.0 40.0 68-69 24.754214136062306 32.0 2.0 37.0 2.0 39.5 70-71 24.36080468718491 31.0 2.0 36.0 2.0 39.0 72-73 24.145237113478654 31.0 2.0 36.0 2.0 39.0 74-75 24.12428225424704 31.0 2.0 35.5 2.0 39.0 76-77 23.894593530364496 31.0 2.0 35.0 2.0 38.0 78-79 23.62525751284778 31.0 2.0 35.0 2.0 37.0 80-81 23.250106534166292 31.0 2.0 35.0 2.0 37.0 82-83 23.349182910805297 31.0 2.0 35.0 2.0 36.5 84-85 23.196223728648057 31.0 2.0 35.0 2.0 36.0 86-87 23.068952414915948 31.0 2.0 35.0 2.0 36.0 88-89 22.914421886973486 31.0 2.0 35.0 2.0 35.5 90-91 22.784389175810297 31.0 2.0 35.0 2.0 35.0 92-93 22.662224337869592 31.0 2.0 35.0 2.0 35.0 94-95 22.55941011116701 31.0 2.0 34.0 2.0 35.0 96-97 22.44226100260458 31.0 2.0 34.0 2.0 35.0 98-99 22.35298616727483 31.0 2.0 34.0 2.0 35.0 100-101 22.2469597298198 30.5 2.0 34.0 2.0 35.0 102-103 21.26551697329402 29.0 2.0 33.5 2.0 34.5 104-105 21.999596417267927 30.0 2.0 34.0 2.0 35.0 106-107 22.142536744337107 30.5 2.0 34.0 2.0 35.0 108-109 22.09796858051383 31.0 2.0 34.0 2.0 35.0 110-111 22.02531732057152 31.0 2.0 34.0 2.0 35.0 112-113 21.93103352204342 30.5 2.0 34.0 2.0 35.0 114-115 21.829025399443424 30.0 2.0 34.0 2.0 35.0 116-117 21.715970493087354 30.0 2.0 34.0 2.0 35.0 118-119 21.5839818252447 30.0 2.0 34.0 2.0 35.0 120-121 21.395815290186864 30.0 2.0 34.0 2.0 35.0 122-123 21.17499211939044 30.0 2.0 34.0 2.0 35.0 124-125 20.30409176107478 28.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2305159920238964 1101 2 1.3846507837652133 1101 3 1.160510903642411 1101 4 1.205051692421815 1101 5 1.2287159347282035 1101 6 1.1439853794301769 1101 7 1.0577204234645485 1101 8 1.2653446826538008 1101 9 1.6396834987181172 1101 10-11 1.7164592517513704 1101 12-13 1.7609788501278985 1101 14-15 2.0415007924255697 1101 16-17 1.666204888124323 1101 18-19 1.738302890337284 1101 20-21 1.9573703282234298 1101 22-23 1.7036302014559617 1101 24-25 1.589096531185234 1101 26-27 1.7527941655252341 1101 28-29 1.7665589951741474 1101 30-31 1.9358298672409227 1101 32-33 1.985581401627119 1101 34-35 1.7274012693112901 1101 36-37 1.6383162121213708 1101 38-39 1.541218390786053 1101 40-41 1.6794115544065846 1101 42-43 1.7198980101151946 1101 44-45 1.5819971215914812 1101 46-47 1.26784273182259 1101 48-49 1.206277946819366 1101 50-51 1.1387021353500337 1101 52-53 1.1045431685338905 1101 54-55 1.096442602060801 1101 56-57 1.0672508099023617 1101 58-59 0.99913545863431 1101 60-61 1.1015761880651525 1101 62-63 0.8294524026637298 1101 64-65 0.8511472774962172 1101 66-67 0.8522995500079809 1101 68-69 0.7336558261985431 1101 70-71 0.7277802573534835 1101 72-73 0.5923222224331823 1101 74-75 0.6355541708257739 1101 76-77 0.7877733793550945 1101 78-79 0.744524336547876 1101 80-81 0.8531553552199256 1101 82-83 0.8975899309442958 1101 84-85 0.9355393873032654 1101 86-87 0.8772245563743155 1101 88-89 0.8933126227888941 1101 90-91 1.0056983570729088 1101 92-93 0.9763277662281062 1101 94-95 0.8767372890945104 1101 96-97 0.8137618483344866 1101 98-99 0.8936046898468213 1101 100-101 0.8897650706105971 1101 102-103 0.7896041287142843 1101 104-105 0.8651251653181582 1101 106-107 0.9196105084864428 1101 108-109 0.8941189635824074 1101 110-111 0.9251025578121741 1101 112-113 0.8556705745613407 1101 114-115 0.8309138805696392 1101 116-117 0.8400410674616872 1101 118-119 0.7762081850833837 1101 120-121 0.76914649798508 1101 122-123 0.7129641864831378 1101 124-125 0.6044687262987338 1102 1 0.18202397235858925 1102 2 0.5773616988223331 1102 3 0.4726121405508046 1102 4 0.4119481379407368 1102 5 0.33071196050413576 1102 6 0.14027829583233142 1102 7 0.005911749307440317 1102 8 -0.04396847373815049 1102 9 0.6069998720284921 1102 10-11 0.6836853100226001 1102 12-13 0.8258740104013107 1102 14-15 1.0672215553548412 1102 16-17 0.7525510025303817 1102 18-19 0.4963161986251805 1102 20-21 0.904533040957471 1102 22-23 0.8533827726716794 1102 24-25 0.4883688111897584 1102 26-27 0.8710887947425938 1102 28-29 0.4654747889464126 1102 30-31 0.4844222117867254 1102 32-33 0.7592692156009697 1102 34-35 0.6613165684796307 1102 36-37 0.5059527282400218 1102 38-39 0.7628262684315494 1102 40-41 0.8184858442785412 1102 42-43 0.9637201262663098 1102 44-45 0.5343825709599841 1102 46-47 0.459497948317793 1102 48-49 0.3314876099669206 1102 50-51 0.42022502808366724 1102 52-53 0.4519077177160007 1102 54-55 0.40813986713661876 1102 56-57 0.37634542564040885 1102 58-59 0.36945796394631714 1102 60-61 0.3110523644615668 1102 62-63 0.16122483485407813 1102 64-65 0.12691174855627452 1102 66-67 0.12411235311141056 1102 68-69 0.12309386736408356 1102 70-71 0.2892747438785186 1102 72-73 -0.04329205933669655 1102 74-75 0.15941563969434824 1102 76-77 -0.09021394682695316 1102 78-79 -0.013336197102582048 1102 80-81 0.11600183161083422 1102 82-83 0.11867761389339293 1102 84-85 0.12772983590359388 1102 86-87 0.191007310749292 1102 88-89 0.15325932513951557 1102 90-91 0.26198580565583995 1102 92-93 0.30542784253842825 1102 94-95 0.25839057246740893 1102 96-97 0.0464995825194805 1102 98-99 0.23631035992824678 1102 100-101 0.2806397278301489 1102 102-103 0.19272571359194046 1102 104-105 0.23179326325625738 1102 106-107 0.16161083351187955 1102 108-109 0.25294704578014304 1102 110-111 0.25377950318181064 1102 112-113 0.07152406164583525 1102 114-115 0.13268804115611843 1102 116-117 0.2160094403488415 1102 118-119 0.21867818454710886 1102 120-121 0.2199269757326121 1102 122-123 0.03230552805190001 1102 124-125 -0.006290032357739506 1103 1 0.4914438774033769 1103 2 0.6985796125106347 1103 3 0.5654828610293414 1103 4 0.39669327633384555 1103 5 0.5925552873445525 1103 6 0.5168149462814 1103 7 0.4226200066207859 1103 8 0.6554662974380108 1103 9 1.2421627714823238 1103 10-11 1.2696749360483182 1103 12-13 1.1025208990169695 1103 14-15 1.3624551937964249 1103 16-17 1.0360595215434572 1103 18-19 0.9456351035847703 1103 20-21 1.3153775679588868 1103 22-23 1.0828238641571488 1103 24-25 0.8809644090814714 1103 26-27 1.05367177955916 1103 28-29 0.9838314589476731 1103 30-31 1.058548817387674 1103 32-33 1.1936816839076094 1103 34-35 1.0176470505124726 1103 36-37 0.903929265239352 1103 38-39 0.8857957175767446 1103 40-41 1.0160302149799278 1103 42-43 1.09584667898379 1103 44-45 0.9076113878245238 1103 46-47 0.6706128053414524 1103 48-49 0.5024024444416533 1103 50-51 0.5328485972919701 1103 52-53 0.4769286232132366 1103 54-55 0.564585747146964 1103 56-57 0.5833601151262897 1103 58-59 0.539295481317069 1103 60-61 0.6548834340885321 1103 62-63 0.5049498621037536 1103 64-65 0.36156098975190787 1103 66-67 0.2877707673858332 1103 68-69 0.470064112699319 1103 70-71 0.5598175853661864 1103 72-73 0.45476222371032904 1103 74-75 0.6312744716747467 1103 76-77 0.4993282824610894 1103 78-79 0.41856628026769727 1103 80-81 0.5475190216769654 1103 82-83 0.6550989930649429 1103 84-85 0.6849468597540387 1103 86-87 0.6142045811542332 1103 88-89 0.5766960860562769 1103 90-91 0.6243367403711915 1103 92-93 0.5844341051900628 1103 94-95 0.608710012504897 1103 96-97 0.6506469228481215 1103 98-99 0.5433215422116966 1103 100-101 0.4461582447572674 1103 102-103 0.388957362292917 1103 104-105 0.4216540144659646 1103 106-107 0.4894636121710434 1103 108-109 0.5509733171134812 1103 110-111 0.4582980193744355 1103 112-113 0.5381593654760657 1103 114-115 0.5941622487119922 1103 116-117 0.5758651198867568 1103 118-119 0.6392904080473478 1103 120-121 0.6218107841357927 1103 122-123 0.4135458235676772 1103 124-125 0.3895331098899817 1104 1 0.6449388550591273 1104 2 0.9109464092005943 1104 3 0.5851759799997502 1104 4 0.7673753757124615 1104 5 0.36603891284127954 1104 6 0.3953917177447437 1104 7 0.22918801046828818 1104 8 0.5205115812257013 1104 9 0.9703617045892656 1104 10-11 1.015898135706994 1104 12-13 1.0796632797713492 1104 14-15 1.0259658643554666 1104 16-17 0.8686519971283566 1104 18-19 0.9362599373463887 1104 20-21 1.0885152366806672 1104 22-23 1.048427401311855 1104 24-25 1.0281752140063602 1104 26-27 1.233972752906375 1104 28-29 1.24820264270247 1104 30-31 1.297854555240015 1104 32-33 1.1653061717124515 1104 34-35 1.02838971403947 1104 36-37 1.003785246256605 1104 38-39 1.086596032122383 1104 40-41 1.0590750543796261 1104 42-43 1.3311506179332753 1104 44-45 1.2088303154960336 1104 46-47 1.0495705454186286 1104 48-49 0.8027482474178242 1104 50-51 0.8106008625669965 1104 52-53 0.6802091239530981 1104 54-55 0.8086146453940515 1104 56-57 0.8517132396479354 1104 58-59 0.6934374542332904 1104 60-61 0.7368437967062391 1104 62-63 0.6603479639011738 1104 64-65 0.6849759757598406 1104 66-67 0.5066656435705816 1104 68-69 0.39632578738342517 1104 70-71 0.49374748605560725 1104 72-73 0.4514052487911151 1104 74-75 0.45214752580359274 1104 76-77 0.5080385404051597 1104 78-79 0.5253583598840805 1104 80-81 0.6813355364809119 1104 82-83 0.7078202633632174 1104 84-85 0.629864144963193 1104 86-87 0.5685020989143759 1104 88-89 0.6078253200913295 1104 90-91 0.6106848373013349 1104 92-93 0.6189925090989377 1104 94-95 0.5685148382400271 1104 96-97 0.5491893537589974 1104 98-99 0.5651235138034316 1104 100-101 0.5637818745458816 1104 102-103 0.5357403804473257 1104 104-105 0.5863244634968723 1104 106-107 0.6061665301137893 1104 108-109 0.5991632683719388 1104 110-111 0.5488723799967374 1104 112-113 0.5666380585555935 1104 114-115 0.645648333903484 1104 116-117 0.5773709456034339 1104 118-119 0.6057710053366563 1104 120-121 0.5872850398492382 1104 122-123 0.6972672907283801 1104 124-125 0.3981642066996187 1105 1 0.5979777414859875 1105 2 0.8726412586934451 1105 3 0.4789337554163531 1105 4 0.6705020053240958 1105 5 0.6353397438208503 1105 6 0.5037130820533768 1105 7 0.43409504362738005 1105 8 0.5587431378040364 1105 9 0.7680183564303604 1105 10-11 0.774417100159436 1105 12-13 0.8892421035855271 1105 14-15 1.0216928335492454 1105 16-17 0.6522029976320312 1105 18-19 0.7148941603920562 1105 20-21 0.4971495754120774 1105 22-23 0.764293789620833 1105 24-25 0.4209627865101737 1105 26-27 0.7492426032446211 1105 28-29 0.8001174641815751 1105 30-31 0.7914938862560739 1105 32-33 0.9274845876009081 1105 34-35 0.669846233108931 1105 36-37 0.3002485996836626 1105 38-39 0.580695080473685 1105 40-41 0.7577791961071156 1105 42-43 0.8633691411780973 1105 44-45 0.5292407952003018 1105 46-47 0.018610104602565514 1105 48-49 0.14609004360802658 1105 50-51 0.36855336734126 1105 52-53 0.02632328442167875 1105 54-55 0.15372959252567142 1105 56-57 0.23987453938612902 1105 58-59 0.26734911838156705 1105 60-61 0.20536543972851717 1105 62-63 0.1715360208612715 1105 64-65 -0.06778157184484002 1105 66-67 -0.15333623409797426 1105 68-69 -0.3226497441925531 1105 70-71 0.04406237374254829 1105 72-73 0.016215604266694328 1105 74-75 0.06749159299387131 1105 76-77 0.10267428160369718 1105 78-79 0.1114317544774579 1105 80-81 0.19982265175908154 1105 82-83 0.15438435237360437 1105 84-85 0.021938559370873634 1105 86-87 0.13349167829882091 1105 88-89 0.12551384780340769 1105 90-91 -0.033547160662593 1105 92-93 0.10046054334903687 1105 94-95 0.06671365575378374 1105 96-97 0.029533768006494654 1105 98-99 0.14971132550976307 1105 100-101 0.23085633344807732 1105 102-103 0.13666942593317444 1105 104-105 0.08512195471810458 1105 106-107 0.14916723134735577 1105 108-109 0.14684795175786292 1105 110-111 0.1983202920471001 1105 112-113 0.060269781009484547 1105 114-115 0.16749936804501786 1105 116-117 0.20235457162169013 1105 118-119 0.2137009513915018 1105 120-121 0.015673341707152844 1105 122-123 0.008367604503703774 1105 124-125 -0.06635702341355909 1106 1 0.10464904031464073 1106 2 -1.544027625497577 1106 3 -0.16253444282046914 1106 4 0.12201411054316225 1106 5 0.2174546707164673 1106 6 0.31665061192835964 1106 7 0.3750472978640822 1106 8 0.37724320432976555 1106 9 0.8360919191049874 1106 10-11 1.0687562532622472 1106 12-13 1.045223998731121 1106 14-15 1.0232758995476985 1106 16-17 0.6617634843047107 1106 18-19 0.659741865747133 1106 20-21 1.0026278461373046 1106 22-23 0.625078103346322 1106 24-25 0.8378388860326673 1106 26-27 0.7149806199893938 1106 28-29 0.8865457223408342 1106 30-31 0.865204149451575 1106 32-33 1.0122935601972358 1106 34-35 0.7802308996671883 1106 36-37 0.49545258728523933 1106 38-39 0.924403224701468 1106 40-41 0.9661471474595587 1106 42-43 1.1581468713201701 1106 44-45 1.03604662383988 1106 46-47 0.8062109403628526 1106 48-49 0.6197515212676983 1106 50-51 0.687397889316923 1106 52-53 0.5499029600717833 1106 54-55 0.5225598237888178 1106 56-57 0.5163453740130173 1106 58-59 0.4078499832160638 1106 60-61 0.46568456612247644 1106 62-63 0.3892838538471928 1106 64-65 0.4515466168319868 1106 66-67 0.37817996122156927 1106 68-69 0.31024895436704014 1106 70-71 0.43673518048776216 1106 72-73 0.24252825548953538 1106 74-75 0.24485140849584397 1106 76-77 0.10424059297368515 1106 78-79 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2216 18-19 -1.29171919993124 2216 20-21 -1.1974984846991568 2216 22-23 -1.22520580960051 2216 24-25 -1.4154922860121673 2216 26-27 -1.2862793886238286 2216 28-29 -1.162240868544071 2216 30-31 -1.123167964623807 2216 32-33 -1.166958338853501 2216 34-35 -1.4383301786144784 2216 36-37 -1.4263125554491616 2216 38-39 -1.2919505243008693 2216 40-41 -1.3967378736664422 2216 42-43 -0.9732339280513251 2216 44-45 -1.0283134212049418 2216 46-47 -1.3260231221590857 2216 48-49 -1.3762724242982003 2216 50-51 -4.565980647301149 2216 52-53 -4.371042822267736 2216 54-55 -3.329162430353712 2216 56-57 -1.8781378913415452 2216 58-59 -1.6188111028798815 2216 60-61 -1.5574861714251043 2216 62-63 -1.7936678549626528 2216 64-65 -1.7555844438038974 2216 66-67 -1.8552050604300554 2216 68-69 -1.9579230102208456 2216 70-71 -4.12174038575834 2216 72-73 -2.2853722230737006 2216 74-75 -1.8478690946175433 2216 76-77 -1.6076553086883507 2216 78-79 -1.604814380139068 2216 80-81 -1.5785700193654968 2216 82-83 -1.662421730207594 2216 84-85 -1.6445617482783632 2216 86-87 -1.7673817907398934 2216 88-89 -1.8191859899229712 2216 90-91 -1.6235205631342744 2216 92-93 -1.516128705825757 2216 94-95 -1.6914836808033549 2216 96-97 -1.7100707189572901 2216 98-99 -1.790153616329743 2216 100-101 -1.809140483165745 2216 102-103 -1.5848657862130082 2216 104-105 -1.5919631705512174 2216 106-107 -1.651053013875881 2216 108-109 -1.7691161588576314 2216 110-111 -1.6543856887067392 2216 112-113 -1.836706602482245 2216 114-115 -1.7892228336282052 2216 116-117 -1.5740035291589862 2216 118-119 -1.6657063618452028 2216 120-121 -1.8740459755897625 2216 122-123 -1.7077391434842752 2216 124-125 -1.5764367896411393 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 570442.0 3 65287.0 4 37184.0 5 29702.0 6 28357.0 7 30532.0 8 33496.0 9 34937.0 10 36371.0 11 37293.0 12 38855.0 13 38601.0 14 36962.0 15 32208.0 16 26747.0 17 23558.0 18 21205.0 19 19759.0 20 19642.0 21 21224.0 22 26460.0 23 36510.0 24 50429.0 25 63114.0 26 71908.0 27 80550.0 28 91195.0 29 102620.0 30 117847.0 31 134180.0 32 159748.0 33 195933.0 34 245525.0 35 320055.0 36 419304.0 37 391918.0 38 79017.0 39 69.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.525483699349856 18.862433461511568 14.374413912270361 23.237668926868217 2 13.606180949926335 19.93461679328645 42.57177670126801 23.887425555519204 3 18.933804744914035 25.41982100126883 28.08167668673519 27.564697567081947 4 11.75629935265652 16.842025999638665 38.09252789106089 33.309146756643926 5 13.65650573610077 38.613860595570756 33.82429696401733 13.905336704311145 6 31.74572909702732 37.19856634026921 17.317945796854946 13.737758765848518 7 28.326938719398452 32.806536625861504 20.441288190523245 18.425236464216795 8 24.999980060864875 36.93581220205947 19.84143336327748 18.222774373798167 9 25.41624484778364 17.676089887831626 19.822022561284257 37.085642703100476 10-11 24.007594411350752 28.024007607696653 27.407091217165036 20.561306763787552 12-13 24.988465594516214 25.13773515304083 27.75154040124848 22.122258851194477 14-15 22.011812346424612 26.581474302471346 25.43956718720594 25.967146163898104 16-17 22.01511422032215 29.833783438136336 25.763905913148044 22.387196428393473 18-19 22.021228438164616 28.525923112235446 27.803121666526227 21.64972678307371 20-21 22.76687979758875 27.501357900602606 27.953747785728268 21.778014516080376 22-23 22.46099272207985 27.378025283099277 27.58397004555828 22.57701194926259 24-25 22.339974892288716 27.37213708624279 27.710871878233583 22.577016143234907 26-27 22.29423105028527 27.45019553158433 27.761428992984747 22.494144425145652 28-29 22.18377894519184 27.566363378800823 27.790626656127692 22.459231019879645 30-31 22.288327196638154 27.387588069536488 27.772238151691987 22.551846582133376 32-33 22.210527281163678 27.236252090236267 27.749060265926033 22.80416036267402 34-35 22.601848379031296 27.24093138788657 27.58274107534202 22.574479157740114 36-37 22.50127081836983 27.269822441496515 27.469087824776135 22.759818915357517 38-39 22.40640093648902 27.335182105276868 27.765004304734255 22.49341265349986 40-41 22.58206706162221 27.181042027566065 27.758560826237638 22.478330084574083 42-43 22.230291731377925 27.111435842347685 27.86487970490071 22.79339272137368 44-45 22.556043377481817 27.366584158185596 27.34149541406195 22.73587705027064 46-47 22.579765667901487 27.594084073147503 26.891257847287264 22.93489241166375 48-49 22.675517018662458 27.49482838502778 26.950435273994945 22.879219322314817 50-51 22.59142997687692 27.62780212213589 26.978410574590338 22.80235732639685 52-53 22.82595463106843 27.601781329426334 26.737816887968368 22.834447151536867 54-55 22.756968852369045 27.506832742360533 26.834355614622567 22.90184279064786 56-57 22.647552961809666 27.686102352855663 26.906689597371848 22.75965508796283 58-59 22.753658904358005 27.398675600506696 26.80344644113304 23.04421905400226 60-61 22.84626652981096 27.37604427215684 26.996409198563683 22.78127999946852 62-63 22.768452200728866 27.514999610071918 26.910524723632655 22.80602346556656 64-65 22.73765310238072 27.344022756584152 26.85249297359112 23.065831167444014 66-67 22.662398825967447 27.578354624938285 26.71973439745088 23.039512151643393 68-69 22.665825186307707 27.455900323100295 26.855316077849594 23.022958412742398 70-71 22.69345853859739 27.356057229733903 26.896845802384593 23.05363842928411 72-73 22.902754500911147 27.394561493936344 26.656082563442528 23.04660144170998 74-75 22.684562813552454 27.49412240641471 26.75627224434427 23.06504253568857 76-77 22.900947306224122 27.3451780264064 26.801412138782382 22.952462528587095 78-79 22.860469719573775 27.391142043741123 26.74712752093333 23.001260715751773 80-81 22.621558527175914 27.533868504891196 26.827860293837276 23.01671267409561 82-83 22.73288071639253 27.650585921930222 26.68641883453352 22.93011452714373 84-85 22.79004845953208 27.39823405271166 26.792709097474095 23.01900839028216 86-87 22.728516952226762 27.32577715921409 26.992677603267108 22.953028285292035 88-89 22.845808785035302 27.352198650541194 26.849965054384946 22.95202751003856 90-91 22.740560881365262 27.509808860737294 26.89231430656151 22.857315951335934 92-93 22.711018754107805 27.484740922175614 26.87452395304212 22.92971637067446 94-95 22.631094890685542 27.46322132637504 26.853604354532234 23.05207942840718 96-97 22.822550460200322 27.375843705019502 26.74677283120691 23.05483300357326 98-99 22.632342140199125 27.360642695353725 26.69295316102247 23.314062003424677 100-101 22.5319750748309 27.451373696478456 26.88399652868043 23.132654700010217 102-103 22.561180937811372 27.279951543745778 26.96645563177712 23.192411886665734 104-105 22.487377026380624 27.253003697195282 27.081806008098415 23.17781326832568 106-107 22.452689150077852 27.375592029079748 26.972189295739625 23.199529525102776 108-109 22.43793082008002 27.38020205863644 26.76612650798653 23.41574061329701 110-111 22.359615915152904 27.40896806319434 26.982437353634097 23.24897866801866 112-113 22.554885792448285 27.400170160499655 26.94808670376056 23.096857343291504 114-115 22.386570036863045 27.524141070871718 26.859448154005612 23.229840738259632 116-117 22.25158513930326 27.416816960323136 27.00047508689229 23.33112281348131 118-119 22.307418542638295 27.447239957930076 26.84020068204273 23.405140817388904 120-121 22.314018090943375 27.481794859537583 26.915005497394713 23.28918155212433 122-123 22.34718003501422 27.37165824516356 26.875944438932382 23.405217280889836 124-125 22.407891007491124 27.263640200829357 26.816791952587653 23.511676839091862 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5015.0 1 3442.0 2 1429.5 3 897.5 4 783.5 5 747.5 6 787.0 7 929.5 8 1111.5 9 1249.0 10 1302.0 11 1387.0 12 1534.5 13 1698.5 14 1897.0 15 2083.5 16 2278.0 17 2514.0 18 2821.5 19 3280.5 20 3909.5 21 4853.5 22 6149.0 23 7624.5 24 9654.0 25 12763.0 26 17024.5 27 22512.5 28 28300.0 29 34575.5 30 42154.5 31 49926.5 32 57696.0 33 65521.5 34 74438.0 35 83432.0 36 92108.0 37 100888.5 38 107045.5 39 111842.0 40 117021.0 41 122219.5 42 127202.5 43 133167.5 44 139708.5 45 143535.5 46 143601.5 47 143899.0 48 147378.0 49 146972.5 50 142214.5 51 137048.5 52 131587.5 53 127127.5 54 121831.0 55 113077.5 56 103252.0 57 94702.5 58 86148.0 59 76198.0 60 64299.5 61 52664.0 62 42293.5 63 33371.0 64 25916.0 65 19980.5 66 15239.5 67 11379.5 68 8801.5 69 6911.0 70 5332.0 71 4166.0 72 3292.0 73 2619.5 74 2029.0 75 1540.5 76 1096.5 77 743.0 78 561.0 79 395.0 80 268.5 81 211.5 82 163.0 83 123.5 84 85.0 85 55.0 86 42.0 87 41.5 88 35.0 89 23.5 90 17.5 91 11.5 92 9.5 93 7.0 94 6.0 95 3.0 96 1.5 97 2.0 98 3.0 99 3.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20099534486821072 2 0.24047799479083748 3 0.2906007943229893 4 0.2775725812100795 5 0.30713150057419664 6 0.22017945501206768 7 0.21298873046298714 8 0.1936188820466447 9 0.19253098645065836 10-11 0.13763205991173716 12-13 0.1615392289845105 14-15 0.17905169467599816 16-17 0.1520798441072145 18-19 0.17817607139142377 20-21 0.17253758811954328 22-23 0.19365868310503445 24-25 0.1844513715975402 26-27 0.23523752210285442 28-29 0.1645641094221311 30-31 0.14470338128564847 32-33 0.18331040792370085 34-35 0.1430848049111322 36-37 0.14384102502053736 38-39 0.17531039518736216 40-41 0.1939107564748362 42-43 0.2583884710662226 44-45 0.1618709044710917 46-47 0.14807320422931353 48-49 0.15003672310987426 50-51 0.15487918521395988 52-53 0.17538999730414165 54-55 0.1635160148845345 56-57 0.1619505065878712 58-59 0.13669010152984654 60-61 0.15008979118772725 62-63 0.13875975656611328 64-65 0.13495212198016104 66-67 0.12268012897665641 68-69 0.1258376796089095 70-71 0.12713784751630783 72-73 0.10556567386906619 74-75 0.10674643860129529 76-77 0.12757565915859503 78-79 0.1329090009828208 80-81 0.14335014530039716 82-83 0.14445130791584676 84-85 0.1322721840485849 86-87 0.15668349986096164 88-89 0.15267685998306066 90-91 0.14089574669969623 92-93 0.14138662641983643 94-95 0.16279959583351905 96-97 0.15003672310987426 98-99 0.14508812485008268 100-101 0.12623569019280695 102-103 0.16384769037111568 104-105 0.12403336496190774 106-107 0.12968511525325147 108-109 0.14015279360975433 110-111 0.11726718503565113 112-113 0.10780780015835516 114-115 0.10608308762813287 116-117 0.11063367530402701 118-119 0.09509799551256333 120-121 0.08819914539167426 122-123 0.11339321535238266 124-125 0.11024893173959283 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3768744.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.31798986911012 #Duplication Level Percentage of deduplicated Percentage of total 1 83.62973389721236 55.46155845351704 2 8.905650265568843 11.812096481796647 3 3.1906303200457025 6.347885677225992 4 1.439190562510148 3.817769005770675 5 0.7666775214711128 2.542225605089786 6 0.4717949954842195 1.8773097438491593 7 0.3122222708749038 1.4494167377754696 8 0.21905840302728508 1.1622010362165547 9 0.1635370933801631 0.9760906171808436 >10 0.832763322941934 9.775248674728369 >50 0.049183770497565255 2.226178816792919 >100 0.01890115296899486 2.0773220513082546 >500 4.5129152739140777E-4 0.16779143733610238 >1k 2.0513248939289252E-4 0.30690566141232456 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.6534038926496467E-5 0.0 10-11 0.0 2.6534038926496467E-5 0.0 5.3068077852992933E-5 0.0 12-13 0.0 2.6534038926496467E-5 0.0 5.3068077852992933E-5 0.0 14-15 0.0 2.6534038926496467E-5 0.0 5.3068077852992933E-5 0.0 16-17 0.0 2.6534038926496467E-5 0.0 5.3068077852992933E-5 0.0 18-19 0.0 2.6534038926496467E-5 0.0 5.3068077852992933E-5 0.0 20-21 0.0 3.98010583897447E-5 0.0 7.96021167794894E-5 0.0 22-23 0.0 5.3068077852992933E-5 0.0 1.1940317516923411E-4 0.0 24-25 0.0 5.3068077852992933E-5 0.0 1.8573827248547528E-4 0.0 26-27 0.0 5.3068077852992933E-5 0.0 2.3880635033846821E-4 0.0 28-29 0.0 7.96021167794894E-5 0.0 5.70481836919674E-4 0.0 30-31 0.0 9.286913624273764E-5 0.0 0.001936984841634242 0.0 32-33 0.0 1.0613615570598587E-4 0.0 0.004683257870526626 0.0 34-35 0.0 1.1940317516923411E-4 0.0 0.008225552067213905 0.0 36-37 0.0 1.3267019463248235E-4 0.0 0.013651763027682434 0.0 38-39 0.0 1.3267019463248235E-4 0.0 0.02350915848887587 0.0 40-41 0.0 1.3267019463248235E-4 0.0 0.03587402062862323 0.0 42-43 0.0 1.3267019463248235E-4 0.0 0.047867406223399626 0.0 44-45 0.0 1.3267019463248235E-4 0.0 0.061081357608794865 1.3267019463248233E-5 46-47 0.0 1.3267019463248235E-4 0.0 0.07742632558751669 2.6534038926496467E-5 48-49 0.0 1.4593721409573057E-4 0.0 0.09627876024479243 2.6534038926496467E-5 50-51 0.0 1.592042335589788E-4 0.0 0.11834181361217425 2.6534038926496467E-5 52-53 2.6534038926496467E-5 1.592042335589788E-4 0.0 0.13907816503323123 2.6534038926496467E-5 54-55 2.6534038926496467E-5 1.592042335589788E-4 0.0 0.16298533410600455 2.6534038926496467E-5 56-57 2.6534038926496467E-5 1.592042335589788E-4 0.0 0.19233198115870964 2.6534038926496467E-5 58-59 2.6534038926496467E-5 1.990052919487235E-4 0.0 0.22406669171479943 2.6534038926496467E-5 60-61 2.6534038926496467E-5 2.1227231141197173E-4 0.0 0.2598478432071799 2.6534038926496467E-5 62-63 2.6534038926496467E-5 2.2553933087522E-4 0.0 0.2980170582029451 2.6534038926496467E-5 64-65 2.6534038926496467E-5 2.5207336980171644E-4 0.0 0.3389325462276026 5.3068077852992933E-5 66-67 2.6534038926496467E-5 2.653403892649647E-4 0.0 0.3859906642637441 6.633509731624116E-5 68-69 2.6534038926496467E-5 2.653403892649647E-4 0.0 0.442800041605373 7.96021167794894E-5 70-71 2.6534038926496467E-5 2.653403892649647E-4 1.3267019463248233E-5 0.5072777561967594 7.96021167794894E-5 72-73 2.6534038926496467E-5 2.653403892649647E-4 2.6534038926496467E-5 0.5749262884398623 7.96021167794894E-5 74-75 2.6534038926496467E-5 2.653403892649647E-4 2.6534038926496467E-5 0.6447904129333274 7.96021167794894E-5 76-77 2.6534038926496467E-5 2.786074087282129E-4 2.6534038926496467E-5 0.7226014820852783 7.96021167794894E-5 78-79 2.6534038926496467E-5 2.9187442819146115E-4 2.6534038926496467E-5 0.8140775812843748 7.96021167794894E-5 80-81 2.6534038926496467E-5 2.9187442819146115E-4 2.6534038926496467E-5 0.9202668050682137 7.96021167794894E-5 82-83 2.6534038926496467E-5 2.9187442819146115E-4 2.6534038926496467E-5 1.0355041361259878 7.96021167794894E-5 84-85 2.6534038926496467E-5 2.9187442819146115E-4 2.6534038926496467E-5 1.153195865784463 7.96021167794894E-5 86-87 2.6534038926496467E-5 2.9187442819146115E-4 2.6534038926496467E-5 1.2781844561477245 7.96021167794894E-5 88-89 2.6534038926496467E-5 2.9187442819146115E-4 2.6534038926496467E-5 1.4211631249031509 7.96021167794894E-5 90-91 2.6534038926496467E-5 2.9187442819146115E-4 2.6534038926496467E-5 1.5842147941064715 7.96021167794894E-5 92-93 2.6534038926496467E-5 2.9187442819146115E-4 2.6534038926496467E-5 1.761992854913998 7.96021167794894E-5 94-95 2.6534038926496467E-5 2.9187442819146115E-4 2.6534038926496467E-5 1.9463513573752955 7.96021167794894E-5 96-97 2.6534038926496467E-5 2.9187442819146115E-4 2.6534038926496467E-5 2.134159284897037 7.96021167794894E-5 98-99 3.98010583897447E-5 2.9187442819146115E-4 2.6534038926496467E-5 2.3471347483405616 7.96021167794894E-5 100-101 5.3068077852992933E-5 2.9187442819146115E-4 2.6534038926496467E-5 2.597231332242254 7.96021167794894E-5 102-103 5.3068077852992933E-5 2.9187442819146115E-4 2.6534038926496467E-5 2.8671355762025756 7.96021167794894E-5 104-105 5.3068077852992933E-5 2.9187442819146115E-4 2.6534038926496467E-5 3.144005005381103 7.96021167794894E-5 106-107 5.3068077852992933E-5 2.9187442819146115E-4 2.6534038926496467E-5 3.4189772507763863 7.96021167794894E-5 108-109 5.3068077852992933E-5 2.9187442819146115E-4 2.6534038926496467E-5 3.7172861834075226 7.96021167794894E-5 110-111 5.3068077852992933E-5 2.9187442819146115E-4 2.6534038926496467E-5 4.054666488357925 7.96021167794894E-5 112-113 5.3068077852992933E-5 3.051414476547094E-4 2.6534038926496467E-5 4.420265743706657 7.96021167794894E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8250 0.0 43.538853 1 ATCAACG 9775 0.0 36.68394 3 TCAACGC 10065 0.0 35.568268 4 CAACGCA 10130 0.0 35.046963 5 AACGCAG 10190 0.0 34.16892 6 TATCAAC 10710 0.0 33.481373 2 GTACATG 12650 0.0 33.10384 1 GGTATCA 5280 0.0 32.94295 1 TACATGG 12950 0.0 31.231745 2 ACATGGG 13335 0.0 29.168657 3 ACGCAGA 11595 0.0 28.585985 7 CGCAGAG 11620 0.0 27.137094 8 GCAGAGT 13400 0.0 21.978296 9 AGTACAT 9700 0.0 20.633068 2 CATGGGG 6720 0.0 20.475983 4 CATGGGA 7850 0.0 19.88079 4 CGCATAG 490 0.0 19.427528 8 AGAGTAC 12675 0.0 19.361963 10-11 GAGTACA 11580 0.0 19.1873 1 TACCTGG 3725 0.0 18.389412 2 >>END_MODULE