FastQCFastQC Report
Thu 26 May 2016
SRR1364611_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364611_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3768744
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73280.1944414372533661No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59980.15915116548112582No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39410.10457064740932258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71200.048.701631
GTATCAA115200.046.2882841
ATCAACG145800.036.115863
TCAACGC148900.035.36634
TATCAAC151500.034.8605772
CAACGCA150900.034.3416255
AACGCAG154550.033.7904936
GTACATG135800.031.7204281
TACATGG141150.029.106622
ACGCAGA181950.028.308857
CGCAGAG185050.027.7702568
ACATGGG145950.027.1507823
GCAGAGT209700.023.7685419
GAGTACT119000.022.44709812-13
GGACCGA8500.021.7173426
AGTACTT126650.021.63203412-13
CATGGGG73050.021.5042724
GTACTTT124750.021.34084914-15
AGAGTAC197850.021.1261810-11
CATGGGA84450.020.2924214
Produced by FastQC (version 0.11.5)